Developing a CRISPR-based forward-genetic screening method in embryonic zebrafish

Information

  • Research Project
  • 9784572
  • ApplicationId
    9784572
  • Core Project Number
    R15HD098969
  • Full Project Number
    1R15HD098969-01
  • Serial Number
    098969
  • FOA Number
    PAR-18-834
  • Sub Project Id
  • Project Start Date
    8/1/2019 - 5 years ago
  • Project End Date
    7/31/2022 - 2 years ago
  • Program Officer Name
    TOYAMA, REIKO
  • Budget Start Date
    8/1/2019 - 5 years ago
  • Budget End Date
    7/31/2022 - 2 years ago
  • Fiscal Year
    2019
  • Support Year
    01
  • Suffix
  • Award Notice Date
    7/29/2019 - 5 years ago
Organizations

Developing a CRISPR-based forward-genetic screening method in embryonic zebrafish

Abstract One of the best methods for identifying novel genes and mutations affecting embryonic development is forward genetic screening. However, these screens require a large number of animals to be screened to identify a single novel mutant due to the inefficiency of chemical mutagenesis. This project will use a CRISPR library generated from mRNA to limit the mutations to genes expressed in the tissue and time point of interest. We will test this method using both F0 and F2 cardiovascular screens for heart phenotypes in zebrafish, potentially providing several novel mutants for further study. In addition to the novel mutants identified in the screen, this method will be able to be adapted to any tissue in any species, reducing the resources needed to conduct forward genetic screens and increase the speed of gene discovery in a number of species and developmental processes.

IC Name
EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
  • Activity
    R15
  • Administering IC
    HD
  • Application Type
    1
  • Direct Cost Amount
    298000
  • Indirect Cost Amount
    149000
  • Total Cost
    447000
  • Sub Project Total Cost
  • ARRA Funded
    False
  • CFDA Code
    865
  • Ed Inst. Type
    SCHOOLS OF ARTS AND SCIENCES
  • Funding ICs
    NICHD:447000\
  • Funding Mechanism
    Non-SBIR/STTR RPGs
  • Study Section
    ZRG1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    BRIGHAM YOUNG UNIVERSITY
  • Organization Department
    PHYSIOLOGY
  • Organization DUNS
    009094012
  • Organization City
    PROVO
  • Organization State
    UT
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    846021001
  • Organization District
    UNITED STATES