DEVELOPMENT OF AN ANTIGEN CAPTURE ASSAY FOR HTLV-I

Information

  • Research Project
  • 3488960
  • ApplicationId
    3488960
  • Core Project Number
    R43AI027612
  • Full Project Number
    1R43AI027612-01
  • Serial Number
    27612
  • FOA Number
  • Sub Project Id
  • Project Start Date
    3/1/1989 - 35 years ago
  • Project End Date
    8/31/1989 - 35 years ago
  • Program Officer Name
  • Budget Start Date
    3/1/1989 - 35 years ago
  • Budget End Date
    8/31/1989 - 35 years ago
  • Fiscal Year
    1989
  • Support Year
    1
  • Suffix
  • Award Notice Date
    1/30/1989 - 36 years ago

DEVELOPMENT OF AN ANTIGEN CAPTURE ASSAY FOR HTLV-I

Phase I funding will support the development of a prototype Elisa antigen capture kit to measure HTLV-1 is linked to the development of adult T- cell leukemia and to tropical spastic paraparesis, a neurological disease with interest to researchers on multiple sclerosis. Tracking the progress of HTLV-I infection in tissue cultures is more difficult than tracking HIV infection: HTLV-I seldom kills the cells it infects and the reverse transcriptase (RT) it produces is not very active in current RT assays. Therefore a commercial kit which measures the core proteins produced during infection will be valuable in studies of HTLV-I in tissue culture. The prototype assay will use p19 and p24 antibodies available in-house as capture antibodies. Hyperimmune rabbit sera specific for purified p19 and p24 will be developed and used as the detector antibodies. The prototype assay will be more sensitive than RT assays in tracking HTLV-1 infection but its sensitivity will be limited by the current lack of very high affinity monoclonal antibodies for p19 and p24. Production of high affinity monoclonal antibodies will begin Phase I.

IC Name
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
  • Activity
    R43
  • Administering IC
    AI
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    856
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    SSS
  • Study Section Name
  • Organization Name
    BIOTECH RESEARCH LABORATORIES, INC.
  • Organization Department
  • Organization DUNS
  • Organization City
    ROCKVILLE
  • Organization State
    MD
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    20850
  • Organization District
    UNITED STATES