DEVELOPMENT OF CELL LINES EXPRESSING OXYGEN GENERATORS

Information

• Research Project
• 2308739

• ApplicationId

  2308739

• Core Project Number

  N44ES022002

• Full Project Number

  N44ES022002-002

• Serial Number

  22002

• FOA Number

• Sub Project Id

• Project Start Date

  6/30/1992 - 32 years ago

• Project End Date

  6/29/1995 - 29 years ago

• Program Officer Name

• Budget Start Date

  6/29/1993 - 31 years ago

• Budget End Date

  6/29/1994 - 30 years ago

• Fiscal Year

  1993

• Support Year

• Suffix

• Award Notice Date

  -

Organizations

• GENTEST CORPORATION

The object of the proposed research is to develop mammalian cells in vitro, which overproduce oxygen radicals and therefore can be used as model systems for studying oxygen radical effects and methods of protecting against oxygen radical damages. The cell lines will be developed by constructing vectors containing cDNAs which code for oxygen radical producing enzymes and then transfecting the vectors into the human lymphoblastoid cell line AHH-1. The cDNAs will be under the control of regulatable promoters so that the levels of oxygen radical production can be regulated. These cell lines will then be used to study the effects of oxygen radicals on various cellular processes, including DNA damage and to identify agents which protect against oxygen radical damage.

IC Name

• Activity

  N44

• Administering IC

  ES

• Application Type

  5

• Direct Cost Amount

• Indirect Cost Amount

• Total Cost

• Sub Project Total Cost

• ARRA Funded

• CFDA Code

• Ed Inst. Type

• Funding ICs

• Funding Mechanism

• Study Section

• Study Section Name

• Organization Name

  GENTEST CORPORATION

• Organization Department

• Organization DUNS

• Organization City

  WOBURN

• Organization State

  MA

• Organization Country

  UNITED STATES

• Organization Zip Code

  01801

• Organization District

  UNITED STATES