DEVELOPMENT OF CELL LINES

Information

• Research Project
• 2307779

• ApplicationId

  2307779

• Core Project Number

  N43ES001001

• Full Project Number

  N43ES001001-000

• Serial Number

  1001

• FOA Number

• Sub Project Id

• Project Start Date

  6/8/1990 - 34 years ago

• Project End Date

  -

• Program Officer Name

• Budget Start Date

  6/8/1990 - 34 years ago

• Budget End Date

  12/7/1990 - 33 years ago

• Fiscal Year

  1990

• Support Year

• Suffix

• Award Notice Date

  -

Organizations

• GENTEST CORPORATION

We propose to develop human cell lines expressing rat cytochrome P450 cDNAs. Two rat cytochrome P450 cDNAs, encoding P450IIB1 and P450IID1, will be transfected into the AHH-1 TK+/- line of human lymphoblastoid cells. Vectors containing the Epstein Barr virus origin of replication will be used in this transfection. Recombinant cell populations will be isolated and specific cytochrome P450 catalyzed reactions will be measured in recombinant and control cell populations. The recombinant cell lines will also be used in gene mutation assays. Promutagens known to be activated by the specific P450 forms will be tested. The eventual goal of this program is to develop a panel of cell lines expressing rat cytochrome P450s. This panel can be used to analyze rat foreign compound metabolism with the ability to relate metabolite formation with the induction of genotoxicity in the same cell line. Additional applications include analysis of strain or sex specific cytochrome P450s and also comparison to human P450s in the same cell system.

IC Name

• Activity

  N43

• Administering IC

  ES

• Application Type

  1

• Direct Cost Amount

• Indirect Cost Amount

• Total Cost

• Sub Project Total Cost

• ARRA Funded

• CFDA Code

  114

• Ed Inst. Type

• Funding ICs

• Funding Mechanism

• Study Section

• Study Section Name

• Organization Name

  GENTEST CORPORATION

• Organization Department

• Organization DUNS

• Organization City

  WOBURN

• Organization State

  MA

• Organization Country

  UNITED STATES

• Organization Zip Code

  01801

• Organization District

  UNITED STATES