Development of Ultra-sensitive Immunoassay Techniques and Immunosensors Based on Enzyme Amplification and the Avidin-Biotin Interaction

Information

  • NSF Award
  • 9123718
Owner
  • Award Id
    9123718
  • Award Effective Date
    5/1/1992 - 32 years ago
  • Award Expiration Date
    10/31/1995 - 29 years ago
  • Award Amount
    $ 110,000.00
  • Award Instrument
    Continuing grant

Development of Ultra-sensitive Immunoassay Techniques and Immunosensors Based on Enzyme Amplification and the Avidin-Biotin Interaction

This Research in Undergraduate Institutions (RUI) project is in the general area of analytical and surface chemistry and in the subfield of bioanalysis. It is jointly supported by the Division of Chemistry and the Experimental Program to Stimulate Competitive Research (EPSCoR) Office. During the tenure of this three-year continuing grant, Professor Asouzu and his students will develop and study enzyme immunoassay methods and immunosensors. The incorporation of avidin, streptavidin, biotin, and their enzyme and antibody conjugates into immunoassays and immunosensor design will be evaluated. Enzymes and antibodies will be used in both their solubilized and immobilized forms. Enzyme immunoassay techniques will be combined with flow injection analysis and amperometric detection to develop a multi-antigen immunoassay method. %%% This project includes a well-conceived, multi-step paradigm for introducing undergraduate students, including freshmen, to applications of enzymes and enzyme kinetic methods for bioanalysis. The research is expected not only to lead to new developments in this rapidly expanding field, but also to contribute strongly to the research and training infrastructure of Troy State University and enable Professor Asouzu, a minority investigator, to initiate a competitive research program.

  • Program Officer
    David J. Curran
  • Min Amd Letter Date
    4/24/1992 - 32 years ago
  • Max Amd Letter Date
    3/18/1994 - 30 years ago
  • ARRA Amount

Institutions

  • Name
    Troy University
  • City
    Troy
  • State
    AL
  • Country
    United States
  • Address
    University Avenue
  • Postal Code
    360820001
  • Phone Number
    3346703102

Investigators

  • First Name
    Moore
  • Last Name
    Asouzu
  • Start Date
    3/18/1994 12:00:00 AM

FOA Information

  • Name
    Health
  • Code
    203000
  • Name
    Trace Contaminants
  • Code
    316000
  • Name
    Chemistry
  • Code
    12

Program Element

  • Text
    ANALYTICAL SEPARATIONS & MEAS.
  • Code
    1974
  • Text
    EXP PROG TO STIM COMP RES
  • Code
    9150

Program Reference

  • Text
    BIOTECHNOLOGY INSTRUMENTATION
  • Code
    9126
  • Text
    UNDERGRADUATE EDUCATION
  • Code
    9178
  • Text
    RES IN UNDERGRAD INST-RESEARCH
  • Code
    9229
  • Text
    SCIENCE, MATH, ENG & TECH EDUCATION