Patent Application
20220138452
This application is based on and derives the benefit of Indian Application 202021047692, filed Nov. 2, 2020, the contents of which are incorporated herein by reference.
The embodiments herein generally relate to a micro-biological colony counters and more particularly, to a device and a method for lighting, conditioning and capturing image(s) of organic sample(s) such as but not limited to micro-organisms.
Microbiology is the study of microscopic organisms such as bacteria, algae, viruses, archaea, fungi and protozoa. Microbiology includes fundamental research on the biochemistry, physiology, cell biology, ecology, evolution and clinical aspects of the micro-organisms. Further, microbiology research includes the formation of colonies of micro-organisms such as bacteria on a growth medium such as agar which is disposed on a petri dish. The microbial colonies are manually counted by a lab technician using microscope devices, wherein the count of the individual colonies is used to determine the effectiveness of various chemicals. Such colony counting is performed in laboratory work, bio-medical facilities and also in pharmaceutical industry. For example, the number of organisms in a blood agar may be counted in a research laboratory or a physician may make a culture of an infections organism during an examination. Further, in quality control of food and beverage industries, the number of micro-organisms present in a product must be regularly checked. Also, in pharma industry the number of micro-organisms in clean room setting must be checked regularly and in compliance with the regulatory norms.
Colony counting within a culture plate involves many number of culture plate transport which includes moving culture plates from incubators to microscope plate and to storage back again. Manual counting of the bacteria colonies is difficult especially for a novice and hence, requires trained laboratory technician. Manual counting of the bacteria colonies is time consuming and involves relatively high labor costs. Additionally, the manual counting of colonies by the lab worker may also results in inaccurate counts of the bacteria colonies. For example, in some instances up to one thousand colonies can be counted and such colonies may be as small as 0.1 millimeters and spaced as close as 0.2 millimeters. As a result, such counting is extremely time consuming, inaccurate, laborious, and exceedingly costly both in time and required skilled labor. Typically, 80% of the petri-dish in a single batch does not have any colony growth. In an analog system, the microbiologist needs to analyze all the petri-dishes with or without colony growth which is time consuming and laborious resulting in fatigue to the lab technician.
Therefore, there exists a need for a device and a method for lighting, conditioning and capturing image(s) of organic sample(s), which obviates the aforementioned drawbacks.
The principal object of embodiments herein is to provide a device for lighting, conditioning and capturing image(s) of organic sample(s).
Another object of embodiments herein is to provide a method of capturing image(s) of organic sample(s).
Another object of embodiments herein is to provide a device for automatic capturing of images of organic sample(s) cultivated on petri dish of different sizes.
Another object of embodiments herein is to provide a device which captures accurate image(s) of organic sample(s) such as but not limited to micro-organisms, where the device is configured for use in colony counting of micro-organisms.
Another object of embodiments herein is to provide a device for capturing images of organic sample(s) related to identification of microbial colonies present in the organic samples(s).
Another object of embodiments herein is to provide a device which has a fixed focus on organic sample(s) to facilitate capturing of accurate image(s) of organic sample(s) thereby enabling repeatability in quality of images captured by the device.
Another object of embodiments herein is to provide a color calibrated imaging device which provides diffused illumination by using polychromatic LED lights, light reflectors and light diffusers for optimal color reproduction of micro-biological colonies contained in organic sample(s).
Another object of embodiments herein is to provide a device for standardizing the imaging process by eliminating entrance of any ambient light by providing a closed photo compartment at all times thereby enhancing the quality of image captured by the device resulting in reliable colony counting.
Another object of embodiments herein is to provide a device in which the micro-organism colonies are illuminated in a calibrated light environment in the dome shaped light reflector which directs the reflection of light from the petri dish towards a digital camera.
Another object of embodiments herein is to provide a device with light diffusers which eliminates image noise so that only focused array of light is selected up by the a digital camera.
Another object of embodiments herein is to provide a device which is configured for automated entry and exit of the organic sample(s) for point accuracy.
Another object of embodiments herein is to provide a device which can capture images of organic sample in different lighting and color conditions thereby improving detection of microbiological colonies by increasing the contrast from the background medium.
Another object of embodiments herein is to provide a device which is adapted to sense the presence of the petri-dish inside a photo compartment and automatically triggers the scan acquisition control.
These and other objects of embodiments herein will be better appreciated and understood when considered in conjunction with following description and accompanying drawings. It should be understood, however, that the following descriptions, while indicating embodiments and numerous specific details thereof, are given by way of illustration and not of limitation. Many changes and modifications may be made within the scope of the embodiments herein without departing from the spirit thereof, and the embodiments herein include all such modifications.
The embodiments are illustrated in the accompanying drawings, throughout which like reference letters indicate corresponding parts in various figures. The embodiments herein will be better understood from the following description with reference to the drawings, in which:
The embodiments herein and the various features and advantageous details thereof are explained more fully with reference to the non-limiting embodiments that are illustrated in the accompanying drawings and detailed in the following description. Descriptions of well-known components and processing techniques are omitted so as to not unnecessarily obscure the embodiments herein. The examples used herein are intended merely to facilitate an understanding of ways in which the embodiments herein may be practiced and to further enable those of skill in the art to practice the embodiments herein. Accordingly, the examples should not be construed as limiting the scope of the embodiments herein.
The embodiments herein achieve a device and a method for lighting, conditioning and capturing images(s) of organic sample(s). Further, embodiments herein achieve the device which captures accurate image(s) of the organic sample(s). Referring now to the drawings
The image capture device (702) is adapted to capture image(s) of the organic sample(s) based on input from an artificial intelligence (AI) based controller system (not shown). The image capture device (702) is disposed above the stationary light reflector (704). The image capture device (702) is mounted on the holder (708). The image capture device (702) is adapted to be moved to one of a plurality of positions in relation to the organic sample(s). For the purpose of this description and ease of understanding, the image capture device (702) is considered to be a camera. Examples of the image capture device (702) includes but not limited to digital camera, multispectral camera, charge coupled device (CCD) type camera, scanner, a thermal camera, an ultraviolet (UV) camera, near-infrared (NIR) camera and so on. However, it is also within the scope of the invention to use any other type of cameras for capturing the images of organic sample(s) without otherwise deterring the intended function of the image capture device (702) as can be deduced from the description and corresponding drawings. The image(s) captured by the image capture device (702) is transferred to the AI based controller system (not shown). The AI based controller system provides an output on type of micro-organism present in the organic sample(s) and number of colonies present in each type of micro-organism based on the image(s) captured by the image capture device (702). It is also within the scope of the invention to configure the image capture device (702) to capture and transfer videos of the organic sample(s) to the AI based controller system for determining the type of micro-organism present in the organic sample(s) and number of colonies present in each type of micro-organism. The AI based controller system provides the output type of micro-organism present in the organic sample(s) and number of colonies present in each type of micro-organism to a display screen or a user interface unit.
In an embodiment, the stationary light reflector (704) is adapted to reflect the illumination of the lights (706) to facilitate uniform distribution of illumination to at least one of a photo compartment (700C), as shown in
In an embodiment, the plurality of lights (706) is adapted to focus an illumination onto the stationary light reflector (704). The plurality of lights (706) is provided within the stationary light reflector (704). The plurality of lights (706) comprises at least one red light, at least one green light and at least one blue light. Each light (706) is a LED light. Each light (706) is near to and facing an inner wall (704W), as shown in
The holder (708) is adapted to hold the image capture device (702). In an embodiment, the holder (708) is adapted to facilitate a change in focus of the image capture device (702). The holder (708) is mounted on the stationary light reflector (704).
In an embodiment, the first light diffuser (710) is adapted to diffuse the illumination of the lights (706) thereby reducing the reflection and glare of the illumination. The first light diffuser (710) defines at least one aperture (710R), as shown in
In an embodiment, the second light diffuser (712) is adapted to diffuse the illumination of the lights (706) thereby reducing the reflection and glare of the illumination. The second light diffuser (712) defines at least one aperture (712R), as shown in
The conditioning of light is achieved by using the stationary light reflector (704) and the light diffusers (710, 712) thereby providing optimal lighting condition in the photo compartment (700C).
At step 74, the method (70) includes reflecting, by the stationary light reflector (704), the illumination of the lights (706) to facilitate uniform distribution of illumination to at least one of a photo compartment (700C) and the organic sample(s).
At step 76, the method (70) includes diffusing, by a first light diffuser (710) and a second light diffuser (712), the illumination of the lights (706) to reduce the reflection and glare of the illumination emitted by the plurality of lights (706).
At step 78, the method (70) includes, capturing, by an image capture device (702), image(s) of the organic sample(s).
The method (70) includes activating, by an artificial intelligence (AI) based controller system, the image capture device (702) to capture image(s) of the organic sample(s).
The method (70) includes controlling, by the AI based controller system, the illumination level of the lights (706) by altering the intensity of illumination from the lights (706).
Further, the method (70) comprises, transferring the captured image(s) of organic sample from the image capture device (702) to the AI based controller system.
Further, the method (70) comprises, providing, by the AI based controller system, an output on type of micro-organism present in the organic sample(s) and number of colonies present in each type of micro-organism based on the image(s) captured by the image capture device (702).
The method (70) includes positioning the organic sample(s) below at least one aperture (710R) of the first light diffuser (710).
Further, the method (70) comprises, moving the image capture device (702) to one of a plurality of positions in relation to the organic sample(s) by changing a focus of the image capture device (702) through a holder (708), where the holder (708) is adapted to hold the image capture device (702).
The technical advantages of the device (700) are as follows. The device is adapted for automatic capturing of images of organic sample(s) of different sizes. The device is adapted to capture accurate image(s) of organic sample(s). The device which has a fixed focus on organic sample(s) to facilitate capturing of accurate image(s) of organic sample(s) thereby enabling repeatability in quality of images captured by the device. The device is a color calibrated imaging device which provides diffused illumination by using polychromatic LED lights, light reflectors and light diffusers for optimal color reproduction of micro-biological colonies contained in organic sample(s). The device is adapted for standardizing the imaging process by eliminating entrance of any ambient light by providing a closed photo compartment at all times thereby enhancing the quality of image captured by the device resulting in reliable colony counting. The device is adapted to capture images of organic sample in different lighting and color conditions thereby improving detection of microbiological colonies by increasing the contrast from the background medium. The device is configured for automated entry and exit of the organic sample(s) for point accuracy. The device is adapted to sense the presence of the petri-dish inside the photo compartment and automatically triggers the scan acquisition control. The micro-organism colonies are illuminated in a calibrated light environment in the dome shaped light reflector which directs the reflection of light from the petri dish towards a digital camera. The device is adapted to eliminate image noise so that only focused array of light is selected up by the digital camera.
The foregoing description of the specific embodiments will so fully reveal the general nature of the embodiments herein that others can, by applying current knowledge, readily modify and/or adapt for various applications such specific embodiments without departing from the generic concept, and, therefore, such adaptations and modifications should and are intended to be comprehended within the meaning and range of equivalents of the disclosed embodiments. It is to be understood that the phraseology or terminology employed herein is for the purpose of description and not of limitation. Therefore, while the embodiments herein have been described in terms of embodiments, those skilled in the art will recognize that the embodiments herein can be practiced with modifications within the spirit and scope of the embodiments as described herein.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202021047692 | Nov 2020 | IN | national |
