Claims
- 1. A biosensor for determining the concentration of a substrate in a sample solution, said biosensor comprising:an electrically insulating base plate; an electrode system having a working electrode, a counter electrode and a third electrode to be utilized as an interfering substance detecting electrode, said working electrode, said counter electrode and said third electrode being formed on said base plate; a reaction layer comprising an oxidoreductase and an electron mediator, said reaction layer being formed on said electrode system so as to cover said working electrode and said counter electrode, said reaction layer not covering said third electrode; and a cover member forming a sample solution supply pathway to introduce a sample solution from a sample solution supply port into said reaction layer on said base plate, wherein said third electrode being located closer to said sample solution supply port than said reaction layer and said entire working electrode being located closer to said sample solution supply port than said counter electrode.
- 2. The biosensor in accordance with claim 1, herein said working electrode is located closer to said sample solution supply port than said counter electrode so that the entire exposed area of said working electrode can be filled with a sample solution prior to reaching said counter electrode.
- 3. The biosensor in accordance with claim 1, wherein said working electrode, counter electrode and third electrode are electrically connected to three independent leads.
- 4. The biosensor in accordance with claim 1, wherein said biosensor is disposed with a layer essentially composed of lecithin on an exposed surface of the sample solution supply pathway of said cover member.
- 5. The biosensor in accordance with claim 1, wherein said biosensor further contains a hydrophilic polymer in said reaction layer.
Priority Claims (2)
Number |
Date |
Country |
Kind |
10-089740 |
Apr 1998 |
JP |
|
10-172766 |
Jun 1998 |
JP |
|
Parent Case Info
This application is a Division of No. 09/424,715 filed on Nov. 29, 1999 now U.S. Pat. No. 6,340,428 claims priority under 35 U.S.C. 371 from PCT/JP99/01706, filed on Mar. 31, 1999.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
5264103 |
Yoshioka et al. |
Nov 1993 |
A |
5582697 |
Ikeda et al. |
Dec 1996 |
A |
6340428 |
Ikeda et al. |
Jan 2002 |
B1 |
Foreign Referenced Citations (8)
Number |
Date |
Country |
2-310457 |
Dec 1990 |
JP |
3-54447 |
Mar 1991 |
JP |
5-340915 |
Dec 1993 |
JP |
6-109693 |
Apr 1994 |
JP |
8-320304 |
Dec 1996 |
JP |
9-101280 |
Apr 1997 |
JP |
9-201337 |
Aug 1997 |
JP |
10-19832 |
Jan 1998 |
JP |