Claims
- 1. A purification device comprising:
a substrate comprising ion-exchange material and size exclusion resin, wherein the ion-exchange material at least partially contacts the size-exclusion resin.
- 2. The device of claim 1, wherein the ion-exchange material comprises cationic-exchange particles.
- 3. The device of claim 1, wherein the ion-exchange material comprises anionic-exchange particles.
- 4. The device of claim 1, wherein the device further comprises a polymeric material.
- 5. The device of claim 4, wherein the polymeric material comprises polystyrene, a co-polymer of polystyrene, a petroleum based polymer, a petroleum based co-polymer, a petroleum-based homopolymer, or a combination thereof.
- 6. The device of claim 5, wherein the polymeric material forms a support with a protrusion extending therefrom, wherein the substrate is affixed to at least a distal end of the protrusion.
- 7. The device of claim 6, wherein the ion-exchange material comprises a cation-exchange material and an anionic-exchange material.
- 8. The device of claim 7, wherein the substrate is at least one of sulfonic acid-treated and heparin-treated.
- 9. The device of claim 1, wherein the ion-exchange material is ion-exchange particles, and wherein said ion-exchange particles are micro-encapsulated by the size-exclusion resin.
- 10. The device of claim 1, wherein the ion-exchange material is ion-exchange particles, and wherein said ion-exchange particles are encapsulated in the size-exclusion resin.
- 11. The device of claim 10, wherein the size-exclusion resin comprises a neutrally-charged cross-linked product of two or more reactive monomeric units.
- 12. The device of claim 10, wherein the size-exclusion resin comprises the reaction product of an acrylamide.
- 13. The device of claim 10, wherein the size-exclusion resin comprises at least one of a poly((meth)acrylamide material, a poly(N-methyl (meth)acrylamide) material, a poly(N,N-dimethylacrylamide) material, a poly(N-ethyl (meth)acrylamide) material, a poly(N-n-propyl (meth)acrylamide) material, a poly(N-iso-propyl (meth)acrylamide) material, a poly(N-ethyl-N-methyl (meth)acrylamide) material, a poly(N,N-diethyl (meth)acrylamide) material, a poly(N-vinylformamide) material, a poly(N-vinylacetamide) material, a poly(N-methyl-N-vinylacetamide) material, a poly(vinyl alcohol) material, a poly(2-hydroxyethyl (meth)acrylate) material, a poly(3-hydroxypropyl (meth)acrylate) material, a poly(vinylpyrrolidone) material, a poly(ethylene oxide) material, a poly(vinyl methyl ether) material, a poly(N-(meth)acrylylcinamide) material, a poly(vinyloxazolidone) material, a poly(vinylmethyloxazolidone) material, a poly(2-methyl-2-oxazoline) material, a poly(2-ethyl-2-oxazoline)material, a polymer of poly(ethylene glycol) acrylate, a polymer of poly(ethyleneglycol) methacrylate, a water-soluble polysaccharide material, hydroxymethylcellulose, and hydroxyethylcellulose.
- 14. The device of claim 10, further comprising a support, and wherein the substrate is disposed in or on the support.
- 15. The device of claim 14, wherein the support comprises a sample well.
- 16. The device of claim 14, wherein the support is a portion of a pathway of a microfluidic device.
- 17. The device of claim 10, wherein the substrate is coated on the support.
- 18. The device of claim 17, wherein the support comprises a surface with a protrusion extending therefrom, the protrusion having a terminal end, and the substrate is supported by the terminal end of the protrusion.
- 19. The device of claim 17, wherein the support comprises at least one of polystyrene, a co-polymer of polystyrene, a petroleum-based polymer, a petroleum-based co-polymer, a petroleum-based homopolymer, and combinations thereof.
- 20. The device of claim 9, wherein said size-exclusion resin comprises a reaction product of an acrylamide.
- 21. A method of manufacturing a device, comprising:
providing ion-exchange particles; providing a support including at least one protrusion extending therefrom; and contacting the protrusion with the ion-exchange particles such that the ion-exchange particles are affixed to the protrusion. contacting the ion-exchange particles with a size-exclusion resin to at least one of encapsulate and micro-encapsulate the ion-exchange particles.
- 22. The method of claim 21, wherein the particles include size-exclusion ion-exchange particles.
- 23. The method of claim 21, wherein the at least one protrusion has a glass transition temperature and a melting temperature, and the method further comprises softening the protrusion by heating the protrusion to a temperature of from the glass transition temperature to the melting temperature.
- 24. The method of claim 21, further comprising softening the protrusion by chemically treating the protrusion.
- 25. The method of claim 21, further comprising softening the ion-exchange particles by at least one of heating the ion-exchange particles and chemically-treating the ion-exchange particle.
- 26. The method of claim 21, providing ion-exchange particles comprises providing ion-exchange particles dispersed in a monomer solution that is capable of polymerization.
- 27. The method of claim 21, further comprising treating the substrate with at least one of sulfonic acid and heparin.
- 28. A method of manufacturing a purification device, comprising:
providing a mold; disposing ion-exchange particles in the mold; disposing reactive monomer solution to the mold; and reacting the monomer solution to form a size-exclusion resin that embeds the ion-exchange particles.
- 29. The method of claim 28, further comprising:
providing a support having at least one protrusion extending therefrom; disposing the protrusion into the mold; and reacting the reactive monomer solution to form a size-exclusion resin attached to at least a portion of the protrusion.
- 30. The method of claim 28, wherein the mold is a well.
- 31. A method comprising:
providing a purification device comprising a substrate comprising ion-exchange material and size exclusion resin, wherein the ion-exchange material at least partially contacts the size-exclusion resin; providing a sample solution; and contacting the purification device with the sample solution for a period of time sufficient to remove impurities from the sample solution and form a purified sample solution.
- 32. The method of claim 31, wherein contacting comprises positioning the purification device and the sample solution in a container.
- 33. The method of claim 32, wherein the container is a sample well, a test tube, a receiving well, a column, or a portion of a pathway of a microfluidic device.
- 34. The method of claim 31, wherein the period of time is from one minute to ten minutes.
- 35. The method of claim 31, wherein the period of time is less than five minutes.
- 36. The method of claim 31, wherein the period of time is less than two minutes.
- 37. A kit for purification of a sample solution, wherein the kit comprises:
ion-exchange particles; a reactive monomer composition capable of forming a size-exclusion resin; and a receptacle capable of receiving the ion-exchange particles and the reactive monomer composition.
- 38. The kit of claim 37, further comprising a support.
- 39. The kit of claim 38, wherein the support includes a plurality of protrusions.
- 40. The kit of claim 37, further comprising heparin.
- 41. The kit of claim 37, further comprising sulfonic acid.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] Cross-reference is made to concurrently filed U.S. patent application Ser. No. ______, to Lau et al., entitled “Size-Exclusion Ion-Exchange Particles,” Attorney Docket No. 4885, which claims priority from U.S. Provisional Patent Application No. 60/398,852 filed Jul. 26, 2002. Cross-reference is also made to concurrently filed U.S. patent application Ser. No. ______ to Ramstad et al., entitled “Petal-Array Support for use with Microplates,” Attorney Docket No. 4329 I1 which is a continuation-in-part of U.S. patent application Ser. No. 10/038,974 to Ramstad, filed Jan. 4, 2002. Both of the above-identified concurrently filed and cross-referenced applications and all other patents and patent applications mentioned herein are incorporated herein in their entireties by reference.
Provisional Applications (1)
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Number |
Date |
Country |
|
60398852 |
Jul 2002 |
US |