Patent Application
20210275082
The invention concerns a device for the testing of sniffing of an animal and methods for using the device.
A field of application of the invention is the testing of dogs, or more generally pets (which are nonhuman animals).
The worldwide pet food market is continually growing as the pet population becomes larger and larger over time, especially in developed countries. Facing an increasing public demand for high quality pet foods, the pet food industry is looking for supplying foods which have a high degree of palatability. Olfaction and taste combine to produce flavor, which is critical in determining the consumption of a food. In particular, olfaction is considered to be an integral role in the sensory experience of eating and food choice for dogs.
Dogs' ability to distinguish between different flavored foods requires olfaction. It would be useful to rapidly identify whether animals can quickly and spontaneously discriminate between two odorant substances.
Moreover, identifying the volatile organic compounds that influence olfactory attractivity is a continuous challenge for the pet food industry. Such technology would permit foods, or palatability enhancers added to foods, to be engineered so that appealing volatile organic compounds remain in the final product. In this context, it would be useful to know the concentration of an odorant substance at which an animal shows an interest in an odorant, if interested at all.
However, despite the well-recognized interest of measuring sniffing pattern of pets, such as dogs, devices currently on the market are not fully adequate for testing pets. For example, masks carried by a living being, such as a human, and attached by straps to the head of the living being are not suitable for pets. Strapping an equipment to the pet (as it can be done with humans) may incite fear in an animal, preventing accurate measurement. Further, while a human can be required to remain still, a pet could require physical restraining, and a failure in restraint may lead to an animal pulling on the equipment, getting tangled, injuring itself, or destroying the equipment.
There is thus a need for a device enabling to test sniffing of the pet without physical constraints exerted on the pet, and even more advantageously leaving the pet the possibility to freely quit the device.
There is also a need for an automatic device, in particular able to deliver an odor only when a pet is present, and more advantageously when a pet is breathing normally in the mask, avoiding inaccurate measures.
To satisfy these needs, a device defined in claim 1 is proposed.
The device enables to study pets olfaction through sniffing behaviors in various periods of the life of the pet. The inventors developed a device for automatically delivering an odorant when the pet is detected or when a specific air flow derived data is measured. In order to generally test an pet's (and in particular a dog's) olfaction, this new device enables the analysis of sniffing behaviors of pets in response to odorants delivered mainly ortho-nasally, thanks to relevant sniffing parameters.
Optional features of said device are recited in the dependent claims.
The invention will be more clearly understood from the following description, given solely by way of non-limiting example in reference to the appended drawings, in which:
In
The receptacle 2 is attached to a casing 10, for example intended to be placed on a surface, such as the ground. This casing 10 makes the receptacle easily accessible to pet walking on the same surface. The receptacle 2 can be made in a rigid material or in a flexible material. The device 1, the receptacle 2 and the casing 10 are not intended to be carried by the pet but form a unit mobile relative to the pet. The front opening 21 is for example attached in a front wall 101 of the casing 10, and is opened to the outside and free to be accessed by the pet, so that front opening 21 receives air flow produced by the pet, when the pet places its nose in the cavity 25 of the receptacle 2 through the front opening 21. The front opening 21 is turned for example with an inclined angle ANG comprised between 0° and 90° from bottom of the casing 10 to top of the front wall 101 of the casing 10 for comfortable access to the head of the pet leaning downwards. The casing 10 encloses or carries the other parts of the device 1, which are described below.
In an embodiment, the casing 10 may be opened by moving or raising the front wall 101 or moving other walls of the casing 10. This enables to place said other parts of the device 1 in the casing 10.
An air flow sensor 3 is provided to measure air flow in the receptacle 2, caused by the pet, for example by placing its nose in the receptacle 2 or by breathing and/or sniffing in the receptacle 2. The air flow sensor 3 measures the sniffing pattern of the pet. The air flow sensor 3 is situated in the casing 10. The air flow sensor 3 is connected to an air exit port 22 of the receptacle 2, situated at a distance from the front opening 21 and thus measures air flow in the air exit port 22. For example, in
In an embodiment shown on
An odor line 4 is provided to introduce a selected odorant air stream 41 into the cavity 25 of the receptacle 2 when the odor line is activated. The odor line 4 is situated in the casing 10. The odor line 4 is able to send the odorant air stream 41 carrying a selected odor to the nose of the pet when the latter is inserted in the cavity 25. In an embodiment shown on
The device 1 further comprises a command unit configured to command sending a selected odorant air stream 41 in odor line 4. In the embodiment shown in
In an embodiment shown on
The presence sensor 5 may be an infrared beam sensor 50 for example situated in front of the front opening 21 to detect the presence of the pet in front of the front opening 21, i.e. when the pet inserts its nose in the receptacle 2 through the front opening and breaks the infrared beam 53 emitted by the sensor 50. Of course, the infrared beam sensor 50 could be an infrared beam sensor 50′ situated behind the front opening 21 and behind the wall 101 inside the casing 10, as shown by reference 50′ in
In an embodiment shown on
Of course, the above applies also to the infrared beam sensor 50′ having the emitter 51′ and the detector 52′ situated so as to emit the infrared beam 53′ in the receptacle 2 as shown on
Of the course, the infrared beam sensor could be situated anywhere else in the fixed casing, for example the emitter and detector can be attached directly to the receptacle.
In an embodiment shown on
In an additional or alternative embodiment not shown, the presence sensor 5 can comprise a camera or an RFID system, in which the RFID emitter part is carried by the pet, for example by a collar of the pet and the RFID receiver part is attached to the casing.
In the embodiment shown on
The command unit can be configured to send the selected odorant air stream 41 in the odor line 4 towards the cavity 25 of the receptacle 2 at or shortly, typically in less than 0.5 second, such as 0.25 second, after the instant of detection of the presence of the pet by the presence sensor 5.
Alternatively, the command unit can be configured to send the selected odorant air stream 41 in the odor line 4 towards the cavity 25 of the receptacle 2 after a first prescribed duration T1 after the instant of detection of the presence of the pet by the presence sensor 5. The first prescribed duration T1 may be for example higher than or equal to 0.5 second and lower than or equal to 5 seconds. For example, the first prescribed duration T1 may be equal to 1.5 seconds or 2 seconds. The first prescribed duration T1 serves to balance the air pressure when the odor is presented in the receptacle 2 through the odor line 4. Indeed, when the pet places its nose in the receptacle 2, large variations of pressures occur. It is presumed according to this embodiment that, after the first prescribed duration T1, the nose of the pet is well placed in the receptacle 2 which is sealed, and that the pressure is stable until an inspiration or expiration occurs.
The command unit can be configured to command sending of the selected odorant air stream 41 in the odor line 4 towards the receptacle 2 as a reaction to both the facts occurring that the presence sensor 5 has detected the presence of the pet and that at least one condition is fulfilled (different possible conditions will be detailed hereinafter). If the presence of the pet is detected by the presence sensor 5 but if the conditions are not met, no selected odorant air stream 41 is sent in the odor line 4 towards the receptacle 2.
According to an embodiment of the invention, the air flow sensor 3 comprises an air flow pressure sensor 32 to measure a pressure of the air flow in the air exit port 22.
The device 1 may comprise a speaker configured to emit a sound indicating that the pet has been present in the receptacle 2 for a predetermined period of time. For instance, the speaker is configured to emit said sound until the presence sensor 5 detects that a pet leaves the receptacle 2. This sound may be emitted as soon as the presence sensor 5 detects the presence of the pet in the receptacle 2. Alternatively, the speaker may be configured to emit said sound whenever the presence sensor 5 detects that the pet leaves the receptacle 2.
The receptacle 2 may comprise a seal arranged to prevent the air flow produced by the pet to escape from the receptacle 2 while the pet is present in the receptacle 2. For instance, the seal has an annular shape extending around the front opening 21. The seal is preferably made of rubber. The seal is preferably removable so as to be replaced by another seal having a different inner diameter. This way a seal may be chosen so as to adapt to a specific animal.
In an embodiment shown on
The device 1 enables to correlate the measurement of air flow caused by the pet in the receptacle 2 by the means 3 to the odor present in the odorant air stream introduced in the receptacle 2. The device 1 enables an automatic measurement of air flow caused by the pet in the receptacle 2 by the means 3. The air flow measured by the air flow sensor 3 quantifies the sniffing of the pet to smell the odor presented in the receptacle through the odor line 4.
A method for testing sniffing of a pet using device 1 comprises the following steps.
A pet puts its nose in the receptacle 2 via the front opening 21. As a consequence, an air flow produced by the pet enters the receptacle 2 and reaches exit port 22.
The presence sensor 5 detects the presence of the pet in the receptacle 2.
Besides, the air flow sensor 3 measures the air flow that reached exit port 22 of the receptacle 2.
The command unit checks if one or many predefined conditions are met before any odor stream is sent in the odor line 4 towards the receptacle 2.
Different conditions may be checked by the command unit.
A first condition may be the expiration of a period of time of duration T1 from the detection of the presence of the pet in the receptacle by the presence sensor 5. In other words, the command unit waits for a predetermined time. Once said period of time has expired, the command unit commands the sending of a selected odor stream 41 in the odor line 4 towards the receptacle 2. The prescribed duration serves to balance the air pressure when the odor is presented in the receptacle 2 through the odor line 4. Indeed, when the pet places its nose in the receptacle 2, large variations of pressures occur. It is presumed according to this embodiment that, after the prescribed duration, the nose of the pet is well placed in the receptacle 2 which is sealed, and that the pressure is stable until an inspiration or expiration occurs. The prescribed duration may be for example higher than or equal to 0.5 second and lower than or equal to 5 seconds. For example, the first prescribed duration T1 may be equal to 1.5 seconds or 2 seconds.
A second condition may have to be fulfilled by data acquired by the air flow sensor 3, more precisely pressure data measured by said sensor 3. This second condition is met whenever a pressure of the air flow measured by the air flow sensor 3 drops below a first predetermined threshold. In other words, to trigger the sending of odor stream 41 in the odor line, the command unit must detect that a pressure of the air flow measured by the air flow sensor 3 has dropped below the first predetermined threshold.
This second condition is advantageous in that it allows ensuring that the odor stream 41 is sent into the receptacle 2 after the end of the large variations of pressure caused by the entrance of the nose of the pet in the receptacle 2. Indeed, pressure dropping below the first threshold means that the pressure has become relatively stable in the receptacle 2.
The second condition offers a good alternative to the first condition. But the first condition and the second condition may also be combined. In this case, the first condition has to be fulfilled, then the second condition has to be fulfilled to allow the odor stream 41 to be sent.
A third condition may be met whenever a pressure of the air flow measured by the air flow sensor 3 rises above a second predetermined threshold. In other words, to trigger the sending of odor stream 41 in the odor line, the command unit must detect that a pressure of the air flow measured by the air flow sensor 3 has risen above said second predetermined threshold. The second threshold is preferably greater than the first threshold used in the second condition. Thus, crossing said second threshold is indicative of an inspiration or an expiration of the pet.
The second condition and the third condition may be advantageously combined. In this case, the command unit commands the sending of odor stream 41 if the second condition and the third condition are met sequentially, in that order. In other words, the data acquired by the air flow sensor 3 must first fall below the first threshold, then rise above the first threshold, then rise above the second threshold greater than the first threshold. When this sequence of events occurs, the odor stream 41 sent in the odor line.
The second condition or the third condition may have to be a predetermined number of times (one time or many times) before the command unit commands the sending of the selected odor stream 41.
It is also possible to combine the first condition, the second condition and the third condition. For example, the first condition has to be fulfilled, then the second condition has to be fulfilled, then the third condition has to be fulfilled to allow the odor stream 41 to be sent.
A fourth condition which may be checked by the command device is a condition related to a frequency of inspiration and/or expiration of the animal. If this frequency crosses a predetermined frequency threshold, for instance falls below said frequency threshold or rises above said frequency threshold, the fourth condition is met.
If all conditions checked by the command unit are met, the command unit commands the sending of a selected odorant air stream 41 in the odor line 4 towards the receptacle 2.
Subsequently, when the pet inspires, the pet breaths the odor stream. This causes a variation of pressure in the receptacle 2, which is acquired by the air flow sensor 3. The data produced by the air flow data at this stage is processed by the calculator unit so as to determine a sniffing response of the pet to the selected odorant air stream 41.
According to an embodiment, to the device 1 further determines a minimal concentration threshold of an odorant, above which a pet shows interest, in which at least a mean instantaneous sniffing frequency Fsniff of the pet is determined by the calculator unit 6 of the device 1 for different concentrations of the odorant in the selected odorant air stream 41, wherein the minimal concentration threshold (DILTH1 in example 1) of the odorant is determined by a concentration of odorant above which the mean instantaneous sniffing frequency Fsniff increases above a first determined value FTH1 (determined according to experimental conditions) or decreases below a second determined value FTH2 (determined according to experimental conditions). According to another embodiment, there is provided a method to determine a minimal concentration threshold of an odorant, above which a pet shows interest, in which at least a mean volume of sniffing MAUC of the pet is determined by the calculator unit 6 of the device 1 for different concentrations of the odorant in the selected odorant air stream 41, wherein the minimal concentration threshold DILTH1 of the odorant is determined by a concentration of odorant above which the mean volume of sniffing MAUC increases above a first determined value FTH1 (determined according to experimental conditions) or decreases below a second determined value FTH2 (determined according to experimental conditions).
The device 1 enables to determine a threshold of concentration of one particular odorant substance 42, above which the pet has (positive or negative) interest to the odor, as shown in example 1. In other words, the device according to the invention enables to measure an odor threshold concentration at which dogs notice and investigate the odorant. This may be measured by the volume of sniffing (see AUC below) and/or frequency of sniffing (see Fsniff below) of the pet measured by the air flow sensor 3. The threshold of concentration may be determined, by the fact that there is a sudden important variation or raise or decrease in frequency and/or volume of sniffing, which indicates that the pet accelerates or slows down breathing in presence of the odor.
According to another embodiment, the device 1 discriminates at least two different odorants by a pet, in which at least a mean instantaneous sniffing frequency Fsniff of a sniffing response of the pet is determined by the calculator unit 6 of the device 1 for each of the respective different odorants in the selected odorant air stream 41, wherein the respective mean instantaneous sniffing frequencies Fsniff associated having been determined for the respective different odorants are compared one to another to indicate non-discrimination of the pet between the different odorants when the respective mean instantaneous sniffing frequencies Fsniff are in a same determined range and to indicate discrimination of the pet between the different odorants when the respective mean instantaneous sniffing frequencies Fsniff are not in the same determined range. According to another embodiment, there is a method to test discrimination of at least two different odorants by a pet, in which the mean volume of sniffing MAUC of the pet is determined by the calculator unit 6 of the device 1 for each of the respective different odorants in the selected odorant air stream 41, wherein the respective mean volume of sniffing MAUC having been determined for the respective different odorants are compared one to another to indicate non-discrimination of the pet between the different odorants when the respective mean volume of sniffing MAUC are in the same determined range and to indicate discrimination of the pet between the different odorants when the respective mean volume of sniffing MAUC are not in the same determined range. The invention enables the determination of whether two distinct odorant substances 42 can be olfactively discriminated by the pet or not, as shown in example 2 below. This enables the evaluation of whether two odorant products 42 are perceived by the pet as having similar odors. This may be useful to analyse whether modifications of a process for manufacturing a product have notable effects on the odor of a pet food.
In an embodiment shown on
In experiments with lickometers, odorants are provided via the liquid substance and therefore, the result is a measure of retro-nasal and taste effects. With the device 1 according to the invention, mainly the ortho-nasal effect of odorants is measured.
In an embodiment, the calculator unit 6 may be connected to a computer, which is configured to emit a first continuous when the presence of the pet is detected by the presence sensor 5. After the second prescribed duration T2, the computer is configured to stop emitting of the first continuous tone and to emit a second tone (for example a chirp) different from the first continuous tone. When the presence of the pet is not detected anymore by the presence sensor 5 before the second prescribed duration T2, the computer is configured to stop emitting any tone.
In an embodiment shown on
In an embodiment shown on
In an embodiment, the calculator unit 6 (what concerns the computer unit 6 may be done also by the computer connected to the calculator unit 6 in the disclosure) comprises processing means or a processor or a calculator to calculate the parameters indicated below.
In an embodiment, the calculator unit 6 calculates or determines or identifies from the differential air pressure value ΔP of air flowing through the air exit port 22, having measured by the differential air pressure sensor 32, as sniffing response at least one of:
In an embodiment, the calculator unit 6 calculates as a sniffing response a mean instantaneous sniffing frequency Fsniff (also called sniffing frequency Fsniff or the sniff frequency Fsniff below) in the receptacle 2 from the differential air pressure values ΔP of air flowing through the air exit port 22, having been measured by the differential air pressure sensor 32. The mean instantaneous sniffing frequency Fsniff is a mean of frequency of peaks of the differential air pressure value ΔP.
Embodiments below are described to calculate the following trial parameters as a sniffing response, which are used in the examples described below.
In an embodiment, in case of a positive peak PE of the differential air pressure value ΔP situated between two consecutive zero values of ΔP, the calculator unit 6 identifies this peak as being an inspiration peak PEinsp (corresponding to a single episode of inspiration). In an embodiment, in case of a negative peak PE of the differential air pressure value ΔP situated between two consecutive zero values of ΔP, the calculator unit 6 identifies this peak as being an expiration peak PEexp (corresponding to a single episode of expiration). If the peak flow rate (also measured by the air pressure sensor 32) is less than a first positive predetermined value PE1 (for example 10 L/min) or greater than a second negative predetermined value PE2 (for example −10 L/min for expiration), this flow rate and the corresponding peak of ΔP is rejected by the calculator unit 6 (for example as data in which there was an insufficient seal between the muzzle of the pet and mask 2). The rejections may be made a posteriori, i.e. after having stored all the peaks PE by the calculator unit 6 in a memory.
In an embodiment, the calculator unit 6 calculates an instantaneous inspiration frequency Finst-insp of each inspiration peak PEinsp by identifying the time Tinsp elapsed between successive inspiration peaks and calculating the reciprocal of this time Tinsp as being the instantaneous inspiration frequency Finst-insp, i.e. Finst-insp=1/Tinsp. In an embodiment, the calculator unit 6 calculates an instantaneous expiration frequency Fint-exp of each expiration peak PEexp by identifying the time Texp elapsed between successive expiration peaks and calculating the reciprocal of this time Texp as being the instantaneous expiration frequency Fint-exp, i.e. Fint-exp=1/Texp. In an embodiment, instantaneous inspiration frequencies Finst-insp and/or instantaneous expiration frequencies Fint-exp higher than a third predetermined frequency value (for example 25 Hz) were rejected by the calculator unit 6 (for example as being noise). The rejections may be made a posteriori, i.e. after having stored all the frequencies by the calculator unit 6 in a memory.
In an embodiment, the calculator unit 6 calculates the sniffing frequency Fsniff as being the overall mean of the instantaneous inspiration frequencies Finst-insp and instantaneous expiration frequencies Fint-exp.
In an embodiment, the calculator unit 6 calculates an AUC (area under the curve: called also volume of sniffing) of the differential air pressure value ΔP as the area under a curve between two successive zero crossings for each peak and/or a total area TAUC under the curve of the differential air pressure value ΔP (being the sum of the absolute values of the areas AUC). In an embodiment, the calculator unit 6 determines the sniffing peak maximum Pmax as the maximum of the heights of the peaks. In an embodiment, the calculator unit 6 determines time Tr to reach each peak as the time elapsing from the preceding zero crossing to this peak. In an embodiment, the calculator unit 6 calculates the sniffing frequency Fsniff, the total area TAUC, the sniffing peak maximum Pmax and the times Tr to reach the peaks. In an embodiment, the calculator unit 6 calculates the minimum, the maximum, and the mean of each of: the instantaneous expiration frequencies Fint-exp of the expiration peaks, the instantaneous inspiration frequencies Fint-insp of the expiration peaks, the areas AUC under the curve of the differential air pressure value ΔP, the height HPE of the peaks, the times Tr to reach the peaks.
In an embodiment, the calculator unit 6 calculates a baseline sniffing frequency Fb. In an embodiment, the calculator unit 6 calculates a baseline sniffing frequency Fb by averaging the sniffing frequency Fsniff for each dog across all blank trials for each odorant. In an embodiment, the calculator unit 6 calculates a measure MPBsniff of proportion of baseline sniffing frequency by dividing the sniffing frequency Fsniff for each odorant trial by the baseline sniffing frequency Fb. In an embodiment, the calculator unit 6 calculates a log proportion LOGMPBsniff of baseline sniffing frequency by log transforming this measure MPBsniff of proportion of baseline sniffing frequency. A value of zero of the log proportion LOGMPBsniff of baseline sniffing frequency indicates that sniffing during the odorant trial was identical to sniffing during blank trials. In an embodiment, to evaluate whether significantly more sniffing was observed at a specific dilution, the log proportion LOGMPBsniff of baseline sniffing frequency at each dilution is tested for being significantly greater than zero on a one-sided one-sample t-test. These calculations may be carried out a posteriori by a person using the calculator unit 6.
In an embodiment shown on
For example, the air flow sensor 33 and/or differential pressure sensor 32 and/or temperature sensor 7 is (are) connected to an analog-to-digital converter (for example of 16 bits) of the calculator unit 6, which is connected to a microcontroller of the calculator unit 6, which may be connected to a computer. Of course, further sensors or measurements could be provided.
In an embodiment shown on
In an embodiment shown on
In an embodiment, the second distributing module 94 may be connected to the air pump 99 by at least a fourth air line 81, in order to send air by the pump 99 to the fourth air line 81 and then to the second distributing module 94 and then to the odor line 4, in order to vent the odor line 4 by bypassing the container 8 (valves 95, 96 and 97 set in a closed position not letting air passing through the first air line 91 and the second air line 93), to enable to clean the odor line 4 from odors (blank or cleaning position mentioned below).
In an embodiment, at least one rotameter 83 (as air flow rate regulator) is provided in an air line 830 connected between the air pump 99 and the first distributing module 92, to enable to set different flow rates in the air lines 830, 98, 91, 93 and then into the odor line 4 in the odor sending position. The air line 98 may be connected to the air connection module 87 and to the air line 91 through the first distributing module 92, as described below. Another rotameter 82 (as air flow rate regulator) may be provided in an air line 820 connected between the air pump 99 and the second distributing module 94, to enable to set different flow rate in the air line 81 and then into the odor line 4 in the cleaning position. The rotameter 83 and/or 82 may be connected to the air pump 99 through a filter 85 situated in an air line 850 situated upstream to the air line 830 and/or 820. The filter 85 can be a carbon and particulate filter or a cleaned charcoal filter, in order to retain dirt or other undesirable particulates from air sent to the air lines.
In an embodiment, a three-way valve 86 is provided, having a first access 861 connected to the rotameter 83, a second access 862 connected to the air line 98 and a third access 863 connected to the fourth air line 81. The three-way valve 86 may be set in a first position (odor sending position) for sending the selected odorant air stream 41 on the odor line 4, in which the first access 861 is connected to the second access 862, and a second position (blank position), in which the first access 861 is connected to the third access 863 for sending a flow of air into the fourth air line 81.
The air sending unit 9 is connected by control wires 90 to the calculator unit 6, which controls the air sending unit 9 and the selectors 88, which are formed for example by the first valves 95.
For example, in the embodiment shown, at least two containers 8 containing different odorant substances 42 producing different odors are provided and are each connected by different air lines 91 and 93 to the air sending unit 9. Any one of the containers 8 can be selected on the air lines 91 and/or on the air lines 93 by selectors 88 (formed for example by the valves 95) provided in the air sending unit 9 to receive the air stream from the air sending unit 9, so that the air stream mixed with the selected odor produced by the selected container 8 is sent to the odor line 4.
In an embodiment, the calculator unit 6 is configured to control the valves 95 and the three-way valve 86. In an embodiment, the rotameter(s) 83 and/or 82 may be controlled manually by actuating mechanic control members of it in order to vary the air flow rate in the air lines. In another embodiment, the valves 95, 97 and the three-way valve 86 may be controlled manually by actuating mechanic control members of them.
The odor may be a food odor. The odorant substance 42 may be a piece of food, or a solution of an odorant substance (such as an aqueous solution or oil or a solid substance or a powder) or at least one volatile organic compound or a food odorant substance or any physical support (such as any object or material . . . ), or a pet food, or mixtures of them. The concentration of the odorant substance 42 in the container 8 may have been determined beforehand. The volume of air contained in each container 8 may be determined beforehand. Different odors or different odorant substances 42 or different concentrations of a same odorant substance 42 may be provided in the container(s) and may be tested. For example, the air flow rate of the air stream sent by the air sending unit 9 in the air line 81 can be 1 liter per minute, the air flow rate of the air stream sent by the air sending unit 9 in each air line 91 and 93 may be 0.5 liter per minute. Of course, other values of the air flow rates are possible.
The calculator unit 6 may be connected to a computer to control the trials operated by the device 1. For example, the computer may be configured to control the air sending unit 9 and the selectors 88 to control whether or not an odor is presented by the odor line 4 in the receptacle 2 and/or which one of several odors is presented by the odor line 4 in the receptacle 2 and/or at which concentration or dilution the odor is presented by the odor line 4 in the receptacle 2. The calculator unit 6 may have means to analyze the measurements supplied by the measurements means 3 and by the presence sensors 5 to the calculator unit 6. The calculator unit 6 and/or the computer may comprise a memory to store the instructions sent to the air sending means 9 and/or the selection means 93 and/or the detections of presence by the presence sensor 5 and/or the detections of absence by the presence sensor 5, and/or an identification of the odorant substance 42 which odor is sent in the selected odorant air stream 41 and the measurements 3 supplied by the air flow sensor.
In an embodiment, prior to the start of testing, the air flow sensor 3 was calibrated by passing a known flow rate through the receptacle measured by a rotameter 82 or 83. In an embodiment, the differential pressure readings (ΔP) from the differential pressure sensor 32 were transformed to flow rates in the calculator unit 6 (for example in a proportional manner). Temperature readings from the temperature sensor 7 were transformed to temperature readings following the manufacturer's recommendations.
Device
A testing device as above described was used. The device comprised in particular a receptacle (“mask”), a pneumotachometer as air flow sensor, an odor line, a calculator unit and infrared beam sensors as presence sensor.
Dog Panel
A cohort of eight dogs of diverse breeds, aged 1-6 years, housed at a facility were involved in the study. Dogs were pair-housed in pens and received twice daily play time, walks, and social enrichment.
Dog Training Phase
No odor was provided during the training phase.
Dogs were encouraged to place their nose within the mask. A tone is emitted during the duration the dog keeps its nose in the mask. Once the dog reached a previously determined duration, another sound (such a “chirp”) indicated that he will be rewarded (for example with treats). Across successful trials, the duration is incremented. If the dog exits the mask prior to the predetermined duration T2, the tone would simply stop, and no treat was given. The required duration was then decreased to the previous validated duration. Once «nose in» time exceeds T2=10 seconds, dogs are qualified to start the tests.
Prior to the start of the study, dogs were given a refresher training session to insure dogs' ability to maintain their nose in the mask for T2=10 s.
Dog Testing Phase
In this testing phase, two odorants products were tested: odorant OD1 (pure molecule) and odorant OD2 (food product) as odorant substance 42 (called below odorant) in different volume dilutions DIL.
When the dog was detected by the infrared beams, an odorant was released immediately (0.25 second from the instant of detection).
Once daily, dogs were given one brief testing session of 10 trials. In a single daily session, 5 dilution steps were presented with intervening blank trials (no odorant) providing 10 total trials. The most diluted odorant was presented first and concentration was increased 10-fold across odor trials. Thus, dogs were presented dilutions ranging from 10−12 to 10−3 Mol/L for OD1 over two consecutive days. For OD2, dogs were presented dilutions ranging from 10−12 to 10−1 volume/volume dilution and undiluted condition over three consecutive days (only 3 dilutions the last day).
Data Treatment & Results
Pressure traces of sensor 32 were calibrated such that a value of zero indicated no air flow. The calculation of the trial parameters described above was made by the device according to the invention for odorant OD1 and odorant OD2 in the trials.
This example 1 shows that through device 1 according to the invention it is possible to determine engage attention thresholds of a given odorant.
In this example 2, the device, the dog panel and the dog training phase are the same as in example 1 above.
Dog Testing Phase
When the dog was detected by the infrared beams, an odorant was released immediately (0.25 second from the instant of detection).
The dogs' spontaneous discrimination was compared between two commercial dog foods (X and Y) and a mix of both as odorant substance 42, by using the device of the invention.
In the trials, the comparisons were as follows for the odorant substance 42 (called below odorant):
The first product X in each comparison served as the habituating odor stimulus and the second product served as the dishabituating odor stimulus.
The mix XY was tested to evaluate dogs' ability to spontaneously discriminate between a food and the same food slightly adulterated with the odor of a different product.
Control condition was conducted to insure dishabituation was due to the odorant change and not an unintentional change in the experiment. In this control condition the same odorant (Product X), served as the habituating and dishabituating odorants although they were treated as separate odorants and placed in separate jars 8.
Dogs were given one odor comparison per day which was comprised of several trials (see Table 1 below). Table 1 shows the trial layout and the trial order for each daily session. Dogs were first presented with two blank trials, followed by the habituating stimulus for four trials, and the dishabituating stimulus for two trials. The order of the odor comparisons tested was randomized across dogs. All dogs completed each comparison once, which was then replicated.
Data Treatment & Results
Pressure traces of sensor 32 were calibrated such that a value of zero indicated no air flow. The calculation of the trial parameters leading to the sniffing frequency Fsniff described above was made by the device 1 according to the invention for the above-mentioned odorant substances 42 in the trials. Blank trials served as ‘warm up’ trials to get the dog working on the task before presenting them with the odor and thus the data coming from these trials were not used.
The sniffing frequency Fsniff was calculated for each trial. To identify whether significant dishabituation occurred when the odorant was changed, analyses were focused on the differences in sniffing frequency Fsniff between trials TR6 and TR7 (change of habituation stimulus to dishabituation stimulus). The sniffing frequencies Fsniff for each trial were log transformed to calculate a log value LOGFsniff of the sniffing frequencies Fsniff to normalize the data. A linear mixed-effect model which included random intercepts for participants was fit. Fixed effects included the odor comparison, the trial type (habituation or dishabituation) and their interaction. LSMeans with a post-hoc correction were used to compare the log values LOGFsniff of the sniffing frequencies Fsniff between the habituation and dishabituation trial for each comparison.
No difference was observed on the control trial (product X vs product X: t=1.41, df=110, p=0.16) of
Study 1
Dog Panel
A cohort of four dogs of diverse breeds, aged 1-6 years, housed at a facility were involved in the study. Dogs were pair-housed in pens and received twice daily play time, walks, and social enrichment.
Device and Dog Training Phase
The device and dog training phase are the same as in Example 1 above.
Dog Testing Phase
2 odorants A and B were tested using the device according to the invention compared to blank odor using the device according to the invention.
Dogs received one testing session daily. During a session, dogs received six blocks of five trials (30 trials total). A block of trials followed the pattern of four blank trials (no odorant) followed by one odorant trial (odorant A, odorant B, or blank). The order of odors presented was randomly shuffled. All dogs completed 5 testing sessions.
For this study, an odorant was sent T1=2 seconds after infrared beam sensors 50 detected the presence of the dog's nose (infrared beam not detected anymore by the detector, meaning that a dog is present). The time before the infrared beam was detected again by the detector 52 (meaning that the dog is no more present) was measured. If this time was lower than T2=10 sec, the trial measure was not taken into account and the trial was performed again until the 10 sec time threshold T2 was reached. IR beam sensors 50 thus detected the number of failed trials (detection of dog's presence during less than T2=10 sec).
The sniffing pattern was analyzed thanks to the pneumotachometer 3.
Results
We hypothesized that more preferred odorants would lead to less trials being terminated prematurely (failed trials).
Over the Odor B required 3.5 more exposures before reaching the 10 sec time threshold T2. This shows that the dogs are more likely to voluntarily withdraw from sniffing odorant B than odorant A, suggesting that odorant B is disliked by the dogs.
Study 2
Dog Panel
A cohort of four dogs of diverse breeds, aged 1-6 years, housed at a facility were involved in the study. Dogs were pair-housed in pens and received twice daily play time, walks, and social enrichment.
Device and Dog Training Phase
The device and dog training phase are the same as in Example 1 above.
Dog Testing Phase
2 odorants A and B were tested using the device according to the invention compared to blank odor using the device according to the invention.
Dogs performed 5 sessions of 18 trials each. In each session, dogs were presented with 2 baseline trials followed by one of the randomly selected 3 odorants (blank, Odor A, Odor B).
For this study, an odorant was sent when the dog was detected by the presence sensor and when the first inspiration was detected by a pneumotachometer 3 in the mask 2 (detected ΔP>THR1), at least T3=1.5 sec after infrared beam sensors 5 detected the presence of the dog's nose (infrared beam not detected anymore by the detector 52 meaning that a dog is present). This insured odor presentation was timed with inspiration. If an inspiration was not detected during a trial, the trial was rejected as indicative of an insufficient seal between the dog and the mask 2. The trial was repeated until a sufficient seal was obtained to allow for the recording of an inspiration.
The time before the infrared beam was detected again by the detector 52 (meaning that the dog is no more present) was measured. IR beam sensors thus enable to measure the time in the mask 2.
During baseline trials, dogs were required to keep their nose in the device for 10 sec to receive a treat. However, during odor trials (A or B or blank), the trial ran until the dog removed his nose, at which point, the computer presented a ‘chirp’ tone and the dog was rewarded (even before reaching the 10 sec threshold T2).
The sniffing pattern was analyzed thanks to a pneumotachometer 3.
Data Treatment & Results
We hypothesized that dogs would maintain their nose in the device longer for more desirable odorants and at the opposite, for a shorter time if the odor is perceived negatively.
The device 1 according to the invention allows a rapid probing of large numbers of dogs to evaluate whether dogs spontaneously discriminate between food odors. This is useful when questions arise as to whether changes in production procedures have a noticeable effect on the aroma of the food. Although this could be evaluated using dogs trained in an operant discrimination test, dogs explicitly rewarded and trained to discriminate aromas may show discrimination between very subtle differences that may not represent untrained (or spontaneous) discrimination. This procedure allows rapid probing of untrained dogs' odor perception. The device according to the invention enables to test sniffing of the pet without physical constraints exerted on the pet (contrary to masks carried by an living being and attached by straps to the head of the living being: such masks attached by straps to the head of the pet are not borne by the pet because of fear and being invasive, would stress the pet or injure it, thus would bias the response because of physical activity and accelerated breathing due to stress, would not lead to a correct measured response representative of sniffing and would prevent accurate measurement), then enabling to test sniffing with the pet breathing in a natural way without having its head distorted by the mask, and with leaving the pet the possibility to freely quit the mask, which can be also an interesting parameter of the sniffing response to be measured. Removal of the pet's head from the receptacle 2 between trials enables a flushing of the cavity 25 of the receptacle 2 before sampling the next odor, and thus causes less pollution.
Of course, the aspects, embodiments, features, possibilities and examples of the invention mentioned above may be combined one with another or may be selected independently one from another.
| Number | Date | Country | Kind |
|---|---|---|---|
| 18305837.9 | Jun 2018 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2019/067458 | 6/28/2019 | WO | 00 |
