Claims
- 1. A method to diagnose the presence of Alzheimer's Disease in a subject by detecting a deficiency of a specific neurotrophic activity in said subject as compared to normal controls, wherein said deficiency is detected by the method which comprises:
- extracting the proteins from the hippocampal tissue of said subject;
- assaying said extract for neurotrophic activity with respect to the neuronal system normally associated with said hippocampal tissue; and
- comparing said neurotrophic activity to activity exhibited in the same assay by similar extracts from hippocampel tissue of controls.
- 2. The method of claim 1 wherein the extract is prepared by sonicating said hippocampal tissue in buffer and recovering the extract.
- 3. The method of claim 2 wherein said hippocampal extract is further purified to isolate a protein of 2kd-5kd.
- 4. The method of claim 1 wherein said neurotrophic activity assay comprises treating medial septal nucleus tissue with said extract.
CROSS REFERENCE TO RELATED APPLICATIONS
The present application is a division of application Ser. No. 444,293, filed on Nov. 24, 1982, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
3989819 |
Brockman |
Nov 1976 |
|
Divisions (1)
|
Number |
Date |
Country |
Parent |
444293 |
Nov 1982 |
|