Claims
- 1. A method to diagnose the presence of ALS in a subject by detecting a deficiency of specific neurotrophic activity in said subject as compared to normal controls, wherein said deficiency is detected by the method which comprises
- extracting the proteins from skeletal muscle tissue of said subject;
- assaying said extract for neurotrophic activity with respect to the neuronal system normally associated with said skeletal muscle tissue; and
- comparing said neurotrophic activity to activity exhibited in the same assay by similar extracts from skeletal muscle tissue of controls.
- 2. The method of claim 1 wherein said extract is prepared by homogenizing skeletal muscle in buffer and recovering the extract.
- 3. The method of claim 1 wherein said neurotrophic activity assay comprises treating embryonic ventral cord cells with said extract.
CROSS REFERENCE TO RELATED APPLICATIONS
The present application is a divisional of application Ser. No. 444,293, filed on Nov. 24, 1982, now abandoned.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
3989819 |
Brockman |
Nov 1976 |
|
Divisions (1)
|
Number |
Date |
Country |
Parent |
444293 |
Nov 1982 |
|