DIAGNOSIS OF PARAMYXOVIRUS INFECTIONS

Information

  • Research Project
  • 3488391
  • ApplicationId
    3488391
  • Core Project Number
    R43AI022782
  • Full Project Number
    1R43AI022782-01
  • Serial Number
    22782
  • FOA Number
  • Sub Project Id
  • Project Start Date
    9/30/1985 - 38 years ago
  • Project End Date
    3/31/1986 - 38 years ago
  • Program Officer Name
  • Budget Start Date
    9/30/1985 - 38 years ago
  • Budget End Date
    3/31/1986 - 38 years ago
  • Fiscal Year
    1985
  • Support Year
    1
  • Suffix
  • Award Notice Date
    -
Organizations

DIAGNOSIS OF PARAMYXOVIRUS INFECTIONS

The paramyxoviruses, respiratory syncytial virus (RSV) and parainfluenza virus are the chief respiratory pathogens of human infancy. A specific, quick, easy and economical diagnostic test would greatly aid the clinician in diagnosis and treatment of paramyxovirus infections. The aim of this proposal is to develop a direct non-radioactive DNA:RNA hybridization test to diagnose paramyxovirus infections. Phase I development will be performed using RSV detection in human Hep-2 cell cultures as a model system. Detection of viral RNA will be based on in situ hybridization of non-radioactive biotinylated RSV specific DNA probes. Visualization will involve detection of biotin, based on enzymatic and flurorescent methods. This model will allow for the optimization of sample treatment and hybridization/detection conditions in order to attain an easy, reliable, sensitive and economical diagnostic assay for RSV infection. After feasibility is established, in Phase II, this protocol will be applied to detect other paramyxovirus infections using the appropriate biotinylated DNA probes. The major emphasis of Phase II will be directed towards establishment of this procedure for differential paramyxovirus detection in the clinical laboratory.

IC Name
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
  • Activity
    R43
  • Administering IC
    AI
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    856
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    SSS
  • Study Section Name
  • Organization Name
    ENZO BIOCHEM, INC.
  • Organization Department
  • Organization DUNS
  • Organization City
    NEW YORK
  • Organization State
    NY
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    10013
  • Organization District
    UNITED STATES