This application is based on and claims priority under 35 USC 119 from Japanese Patent Application No. 2016-064332 filed Mar. 28, 2016 and Japanese Patent Application No. 2016-064462 filed Mar. 28, 2016.
The present invention relates to a digital holographic apparatus.
According to an aspect of the invention, there is provided a digital holographic apparatus that includes a first hologram generating unit that generates a first hologram by causing first object light in a first observation direction to interfere with first reference light, the first object light being generated by irradiating an observation object with light having a first wavelength, the first reference light being derived from the light having the first wavelength; a second hologram generating unit that generates a second hologram by causing second object light in a second observation direction that differs from the first observation direction to interfere with second reference light, the second object light being generated by irradiating the observation object with light having a second wavelength, the second reference light being derived from the light having the second wavelength; a first image capturing unit that captures the first hologram; and a second image capturing unit that captures the second hologram.
Exemplary embodiments of the present invention will be described in detail based on the following figures, wherein:
An exemplary embodiment of the present invention is described in detail below with reference to the drawings.
First, an overall structure of a digital holographic apparatus is described.
The hologram generating unit 12 functions as a first hologram generating unit that causes object light that is obtained in a first observation direction by irradiating an observation object with light having a first wavelength and reference light that is derived from the light having the first wavelength to interfere with each other to generate a first hologram, which is an interference fringe. The first image capturing unit 141 is a digital image capturing element, such as a CCD, and captures the first hologram generated by the first hologram generating unit.
The hologram generating unit 12 also functions as a second hologram generating unit that causes object light that is obtained in a second observation direction by irradiating the observation object with light having a second wavelength and reference light that is derived from the light having the second wavelength to interfere with each other to generate a second hologram, which is an interference fringe. Here, the second wavelength differs from the first wavelength. The second observation direction is a direction that intersects the first observation direction. The second image capturing unit 142 is a digital image capturing element, such as a CCD, and captures the second hologram generated by the second hologram generating unit.
Image information about the first hologram and image information about the second hologram are output from the image information processing unit 16. The image information about the first hologram and the image information about the second hologram are associated with each other, and are stored in a storage device (described later), such as a memory, of the image information processing unit 16. The first wavelength and the second wavelength may be any wavelengths as long as they are separable by a wavelength filter or the like. For example, the first wavelength may be in the range of 500 nm to 560 nm of green light, and the second wavelength may be in the range of 650 nm to 780 nm of red light. The first observation direction and the second observation direction may be desired directions in which an observation object is to be observed. The structure of the hologram generating unit 12 is described below.
The image information processing unit 16 generates a first reconstructed image by processing the image information obtained by capturing the first hologram by the first image capturing unit 141, and generates a second reconstructed image by processing the image information obtained by capturing the second hologram by the second image capturing unit 142. As described above, in the digital holographic technology, the digital image capturing elements image the holograms, and the pieces of image information about the captured holograms are reconstructed in a calculator to obtain reconstructed images having three-dimensional structures. As a reconstruction method, a well-known convolution method or Fresnel's method may be used. For example, methods described in “Digital Holography and Wavefront Sensing, Springer (2015)” by U. Schnars, C. Falldorf, J. Watson, and W. Juptner are used.
The image information processing unit 16 is formed as a computer including a central processing unit (CPU), read-only memory (ROM), random access memory (RAM), and a memory. ROM stores a reconstructing operation program for obtaining the reconstructed images having three-dimensional structures by processing the pieces of image information obtained by capturing the holograms. The CPU reads out the reconstructing operation program stored in ROM, and uses RAM as a work area to execute the reconstructing operation program. The display 18 is, for example, a display disposed as a peripheral unit of the computer. The display 18 displays, for example, the generated reconstructed images. As a peripheral unit, an input device, such as a keyboard, may be provided.
In the exemplary embodiment, the second hologram when the observation object is observed from the second observation direction by irradiating the observation object with the light having the second wavelength is captured at the same time that the first hologram when the observation object is observed from the first observation direction by irradiating the observation object with the light having the first wavelength is captured. Therefore, compared to the case in which the observation object is observed from one direction by irradiating the observation object with light having a single wavelength, it is possible to observe the observation object from desired directions, and to enlarge an observation region including observation regions that are observed at the same time. In other words, blind spots where the object to be observed is not observable are reduced.
The phrase “at same time” in the specification not only includes the case in which the image capturing time of the first hologram and the image capturing time of the second hologram exactly overlap, but also the case in which the image capturing time of the first hologram and the image capturing time of the second hologram partly overlap.
Next, a hologram generating unit 12 is described. Here, the description of the hologram generating unit 12 also includes the description of the first image capturing unit 141 and the second image capturing unit 142.
The first unit 1001 includes a first light source 221 that projects light having a first wavelength (λ1); a dividing optical element 241 having a reflecting surface 251 that divides, transmits, or reflects incident light in accordance with an incidence direction; a lens 261; a dividing optical element 281 having a reflecting surface 291 that divides, combines, transmits, or reflects incident light in accordance with an incidence direction; a wavelength filter 301 that only transmits the light having the first wavelength (λ1); a lens 321; the first image capturing unit 141; a reflecting mirror 341; a reflecting mirror 361; and a lens 381. The optical components are disposed on the same substrate.
The second unit 1002 includes a second light source 222 that projects light having a second wavelength (λ2); a dividing optical element 242 having a reflecting surface (not shown) that divides, transmits, or reflects incident light in accordance with an incidence direction; a lens 262; a dividing optical element 282 having a reflecting surface (not shown) that divides, combines, transmits, or reflects incident light in accordance with an incidence direction; a wavelength filter 302 that only transmits the light having the second wavelength (λ2); a lens 322; the second image capturing unit 142; and an optical system (not shown) that forms an optical path for reference light. The optical components are disposed on the same substrate. The optical system that forms the optical path for reference light includes a pair of reflecting mirrors and lenses.
By disposing the first image capturing unit 141 in the first unit 1001 and the second image capturing unit 142 in the second unit 1002, the size of the entire digital holographic apparatus 10 is reduced. As described below, the first image capturing unit 141 is disposed on an optical axis of the object light having the first wavelength, and the second image capturing unit 142 is disposed on an optical axis of the object light having the second wavelength.
As the dividing optical element 241, the dividing optical element 242, the dividing optical element 281, and the dividing optical element 282, beam splitters or the like may be used. As the wavelength filter 301 and the wavelength filter 302, bandpass filters or the like may be used.
In the first unit 1001, the first light source 221, the dividing optical element 241, the lens 261, the dividing optical element 281, the wavelength filter 301, and the lens 321 are disposed in that order along the first observation direction with their optical axes coinciding with each other. The first light source 221 and the dividing optical element 241 are disposed upstream in the first observation direction from the position of the observation object S. The lens 261, the dividing optical element 281, the wavelength filter 301, the lens 321, and the first image capturing unit 141 are disposed downstream in the first observation direction from the position of the observation object S.
The observation object S is held by a holding member (not shown). The lens 261 is disposed such that its focal position is disposed within the observation object S. As described below, the focal position of the lens 262 of the second unit 1002 differs from the focal position of the lens 261. The first image capturing unit 141 is disposed such that its image capturing surface is at the focal position of the lens 321.
The reflecting mirror 341 is disposed at a reference light reflection side of the dividing optical element 241. The reflecting mirror 361 and the lens 381 are disposed at a reference light incident side of the dividing optical element 281. A reflecting surface of the reflecting mirror 341 is inclined at an angle of 45 degrees with respect to an optical axis of the reference light reflected by the dividing optical element 241 such that incident light is reflected in the direction of the reflecting mirror 361. A reflecting surface of the reflecting mirror 361 is inclined at an angle of 45 degrees with respect to the optical axis of the reference light reflected by the reflecting mirror 341.
In the second unit 1002, the second light source 222, the dividing optical element 242, the lens 262, the dividing optical element 282, the wavelength filter 302, and the lens 322 are disposed in that order along the second observation direction with their optical axes coinciding with each other. The second light source 222 and the dividing optical element 242 are disposed upstream in the second observation direction from the position of the observation object S. The lens 262, the dividing optical element 282, the wavelength filter 302, the lens 322, and the second image capturing unit 142 are disposed downstream in the second observation direction from the position of the observation object S.
The lens 262 is disposed such that its focal position is disposed within the observation object S. As described below, the focal position of the lens 261 of the first unit 1001 differs from the focal position of the lens 262. The second image capturing unit 142 is disposed such that its image capturing surface is at the focal position of the lens 322. Although not shown, the optical system that forms the optical path for the reference light is disposed such that the reference light reflected by the dividing optical element 242 is incident upon the dividing optical element 282.
In the exemplary embodiment, the direction of the optical axis of the object light having the first wavelength is defined as the “first observation direction”, and the direction of the optical axis of the object light having the second wavelength is defined as the “second observation direction”. Therefore, the optical axis of the object light having the first wavelength and the optical axis of the object light having the second wavelength intersect each other. In the illustrated exemplary embodiment, the first observation direction and the second observation direction are orthogonal to each other, and the optical axis of the object light having the first wavelength and the optical axis of the object light having the second wavelength are also orthogonal to each other.
Next, an operation of the hologram generating unit 12 is described.
First, the operation of the first unit 1001 is described. As shown in
The light (object light) having the first wavelength and transmitted through the dividing optical element 241 illuminates the observation object S while the object light remains parallel light. That is, the light having the first wavelength and illuminating the observation object S is a “plane wave”. The light that illuminates the observation object S is scattered when it is transmitted through the observation object S.
The object light having the first wavelength and transmitted through the observation object S is collimated by the lens 261, and is incident upon the dividing optical element 281. The object light having the first wavelength and incident upon the dividing optical element 281 is transmitted through the reflecting surface 291 and exits therefrom in the first observation direction. The wavelength filter 301 removes light having wavelengths other than the first wavelength, and the lens 321 gathers the light to irradiate the image capturing surface of the first image capturing unit 141 with the gathered light.
The light (reference light) having the first wavelength and reflected by the dividing optical element 241 illuminates the reflecting mirror 341, is reflected by the reflecting mirror 341 in the direction of the reflecting mirror 361, is reflected by the reflecting mirror 361 in the direction of the lens 381, is gathered by the lens 381, and is incident upon the dividing optical element 281.
The reference light having the first wavelength and incident upon the dividing optical element 281 is reflected by the reflecting surface 291 in the first observation direction. The wavelength filter 301 removes light having wavelengths other than the first wavelength, and the lens 321 collimates the light to irradiate the image capturing surface of the first image capturing unit 141 with the collimated light.
The object light having the first wavelength and illuminating the image capturing surface of the first image capturing unit 141 and the reference light having the first wavelength and illuminating the image capturing surface of the first image capturing unit 141 interfere with each other to generate an interference fringe at the image capturing surface of the first image capturing unit 141. The first image capturing unit 141 captures, as the first hologram, the interference fringe generated at the image capturing surface.
Next, the operation of the second unit 1002 is described. As shown in
The light (object light) having the second wavelength and transmitted through the dividing optical element 242 illuminates the observation object S while the object light remains parallel light. That is, the light having the second wavelength and illuminating the observation object S is a “plane wave”. The light that illuminates the observation object S is scattered when it is transmitted through the observation object S.
The object light having the second wavelength and transmitted through the observation object S is collimated by the lens 262, and is incident upon the dividing optical element 282. The object light having the second wavelength and incident upon the dividing optical element 282 is transmitted through the reflecting surface and exits therefrom in the second observation direction. The wavelength filter 302 removes light having wavelengths other than the second wavelength, and the lens 322 gathers the light to irradiate the image capturing surface of the second image capturing unit 142 with the gathered light.
The light (reference light) having the second wavelength and reflected by the dividing optical element 242 is transmitted through the optical system (not shown), and is incident upon the dividing optical element 282. The reference light having the second wavelength and incident upon the dividing optical element 282 is reflected by the reflecting surface (not shown) in the second observation direction. The wavelength filter 302 removes light having wavelengths other than the second wavelength, and the lens 322 collimates the light to irradiate the image capturing surface of the second image capturing unit 142 with the collimated light.
The object light having the second wavelength and illuminating the image capturing surface of the second image capturing unit 142 and the reference light having the second wavelength and illuminating the image capturing surface of the second image capturing unit 142 interfere with each other to generate an interference fringe at the image capturing surface of the second image capturing unit 142. The second image capturing unit 142 captures, as the second hologram, the interference fringe generated at the image capturing surface.
In the exemplary embodiment, the operation of the first unit 1001 as the first hologram generating unit and the operation of the second unit 1002 as the second hologram generating unit are performed at the same time. Since the wavelength of the light having the first wavelength and the wavelength of the light having the second wavelength differ from each other, it is possible to irradiate the observation object with the light having the first wavelength and the light having the second wavelength to generate the object light having the first wavelength and the object light having the second wavelength. The generated object light having the first wavelength and the generated object light having the second wavelength are separated by, for example, the wavelength filters. Therefore, a region including observation regions that are observed at the same time is enlarged.
In the exemplary embodiment, since the object light illuminates the observation object as a plane wave, a wide range of the observation object is uniformly irradiated. Therefore, the brightness of reconstructed images also becomes uniform. For example, when the inside of a plane of the observation object or a wide range in a depth direction of the observation object is to be photographed, a method of irradiating the observation object with the object light as a plane wave is a suitable method.
In the exemplary embodiment, the optical axis of the object light and the optical axis of the reference light having the same wavelength are coaxial (in-line relationship). However, the optical axis of the reference light may be inclined such that the optical axis of the object light and the optical axis of the reference light intersect each other (off-axis relationship). The off-axis relationship makes it easier for first-order diffracted light and zeroth order diffracted light to be separated from each other, and allows noise that is generated at the reconstructed images by the zeroth order diffracted light to be reduced.
For example, in order to obliquely irradiate the observation object with the reference light having the first wavelength, the dividing optical element 281 only needs to be inclined. Alternatively, a deflecting element that changes the optical path of the reference light only needs to be inserted in front of or in back of the wavelength filter 301. The deflecting element may be, for example, an optical element such as a wedge type prism.
Enlargement of a Region that is Observed
Next, the enlargement of a region that is observed is described.
Therefore, as shown in
Here, the term “focal depth” refers to the “distance from where the focal position is located on an image capturing element in the direction of the optical axis” at which blurring of an optical image formed when the object light is focused is permitted. In addition, the term “depth of field” refers to the “distance from a focal position in the observation object in the direction of the optical axis (that is, a reconstruction distance from a hologram” at which blurring of an optical image formed when the object light is focused is permitted. That is, the range that is indicated by a thick solid double-headed arrow and that extends between a location in front of and a location in back of the focal position 1 in the direction of the optical axis of the object light having the first wavelength corresponds to a depth of field (λ1) based on the object light having the first wavelength.
In the exemplary embodiment, since the optical axis of the object light having the first wavelength and the optical axis of the object light having the second wavelength intersect each other, the directions of the depths of field of the two object light beams differ from each other. Therefore, in order to express the enlargement of the depths of field, the “depth of field” regarding the object light having the second wavelength is defined as a “measured visual field” instead of the “depth of field”. Here, the term “measured visual field” refers to the “length of the observation region in the observation object in the direction of the optical axis of the object light having the first wavelength” at which blurring of an optical image formed when the object light is focused is permitted. The range that is indicated by a thick dotted double-headed arrow with respect to the focal position 2 corresponds to the measured visual field (λ2) based on the object light having the second wavelength.
As shown in
Since the focal position 1 and the focal position 2 in the observation object S and their directions differ from each other, the first observation region (λ1) and the second observation region (λ2) differ in position and orientation. Therefore, compared to the case in which the observation object is irradiated with light having a single wavelength, a region including observation regions that are observed at the same time is enlarged to a region in which the first observation region (λ1) and the second observation region (λ2) are connected to each other. The image information processing unit 16 connects the first observation region (λ1) and the second observation region (λ2) to each other (see
The image information processing unit 16 reads out the image information about the first hologram and the image information about the second hologram that are associated with each other from the storage device, processes the image information about the first hologram to generate the first reconstructed image, and processes the image information about the second hologram to generate the second reconstructed image. By combining the first reconstructed image and the second reconstructed image, the first observation region (λ1) and the second observation region (λ2) are connected to each other. An image in which the observation regions are connected to each other, that is, a composite image of the first reconstructed image and the second reconstructed image is displayed on the display 18.
By combining the depth of field (λ1) and the measured visual field (λ2), the depth of field in the direction of the optical axis of the object light having the first wavelength is increased. When the wavelength is λ, the numerical aperture of the lenses 261 and 262, which are objective lenses, is NA, the diameter of pixels of the image capturing units (that is, the pixel pitch) is p, the number of pixels of the image capturing units is N, and the magnification is M, the depth of field is a range from the focal position and is expressed by the following Formula (1). In Formula (1) below, “N” of the numerator represents the number of pixels:
Formula (1) above indicates the range that allows the resolving power that is determined by the NA of the objective lenses to be maintained. Therefore, since the required resolution depends upon use, formulas for the depth of field are not limited to this formula. The measured visual field is expressed by pN/M.
In associating the image information about the first hologram and the image information about the second hologram with each other and in storing them, the three-dimensional coordinates of the focal position 1 where the object light having the first wavelength is focused and the three-dimensional coordinates of the focal position 2 where the object light having the second wavelength is focused may be stored together. For example, when the holding member that holds the observation object S includes a position sensor or the like, the three-dimensional coordinates of the focal positions of the lenses 281 and 282, which are objective lenses, are determined. The three-dimensional coordinates of the focal position 1 and the three-dimensional coordinates of the focal position 2 are used when combining the first reconstructed image and the second reconstructed image.
In the example shown in
The state of overlap of the two observation regions is not limited to that mentioned above. As shown in
In the first unit 1001, when the numerical aperture is NA1, the diameter of pixel of the image capturing unit is p1, the number of pixels of the image capturing unit is N1, and the magnification is M1, the depth of field (λ1) is a range from the focal position that is expressed by the following Formula (2):
In the second unit 1002, when the diameter of pixel of the image capturing unit is p2, the number of pixels of the image capturing unit is N2, and the magnification is M2, the measured visual field (λ2) is expressed by p2N2/M2.
The first observation direction and the second observation direction may intersect each other at an angle other than 90 degrees. That is, the optical axis of the object light having the first wavelength and the optical axis of the object light having the second wavelength may intersect each other at an angle other than 90 degrees.
The hologram generating unit 12 according to the second exemplary embodiment includes a first unit 100A1 and a second unit 100A2. Except that, in the first unit 100A1, a first light source 22A1 that projects “light having a first wavelength and being s-polarized light” is disposed in place of the first light source 221 that projects light having the first wavelength, and a polarizing filter 30S that transmits only s-polarized light is inserted between a wavelength filter 301 and a lens 321, the first unit 100A1 has the same structural features as the first unit 1001 shown in
Except that, in the second unit 100A2, a second light source 22A2 that projects “light having a second wavelength and being p-polarized light” is disposed in place of the second light source 222 that projects light having the second wavelength, and a polarizing filter 30P that transmits only p-polarized light is inserted between a wavelength filter 302 and a lens 322, the second unit 100A2 has the same structural features as the second unit 1002 shown in
In the second exemplary embodiment, as in the first exemplary embodiment, a first observation direction and a second observation region intersect each other, and an optical axis of object light having the first wavelength and an optical axis of object light having the second wavelength also intersect each other. In the second exemplary embodiment, as in the first exemplary embodiment, the first unit 100A1 and the second unit 100A2 are disposed such that a focal position of a lens 262 differs from a focal position of a lens 261.
Except that the “light having the first wavelength and being s-polarized light” is used in place of the light having the first wavelength, and the “light having the second wavelength and being p-polarized light” is used in place of the light having the second wavelength, the operation of the hologram generating unit according to the second exemplary embodiment is substantially the same as the operation of the hologram generating unit according to the first exemplary embodiment. Therefore, only differences are described.
In the first unit 100A1, object light that has the first wavelength and is s-polarized light, and that is incident upon a dividing optical element 281 is transmitted through a reflecting surface 291, and exits therefrom in the first observation direction. Then, the wavelength filter 301 removes light having wavelengths other than the first wavelength, and the polarizing filter 30S removes light other than the s-polarized light. Then, the lens 321 gathers the light, and an image capturing surface of a first image capturing unit 141 is irradiated with the gathered light.
On the other hand, reference light that has the first wavelength and that is s-polarized light, and that is incident upon the dividing optical element 281 is reflected by the reflecting surface 291 in the first observation direction. Then, the wavelength filter 301 removes light having wavelengths other than the first wavelength, and the polarizing filter 30S removes light other than the s-polarized light. Then, the lens 321 collimates the light, and the image capturing surface of the first image capturing unit 141 is irradiated with the collimated light.
The object light that has the first wavelength and that is s-polarized light, and that illuminates the image capturing surface of the first image capturing unit 141 and the reference light that has the first wavelength and that is s-polarized light, and that illuminates the image capturing surface of the first image capturing unit 141 interfere with each other to generate an interference fringe at the image capturing surface of the first image capturing unit 141. The first image capturing unit 141 captures, as a first hologram, the interference fringe generated at the image capturing surface.
Next, in the second unit 100A2, object light that has the second wavelength and that is p-polarized light, and that is incident upon a dividing optical element 282 is transmitted through a reflecting surface (not shown) and exits therefrom in the second observation direction. Then, the wavelength filter 302 removes light having wavelengths other than the second wavelength, and the polarizing filter 30P removes light other than the p-polarized light. The lens 322 gathers the light, and an image capturing surface of a second image capturing unit 142 is irradiated with the gathered light.
On the other hand, reference light that has the second wavelength and that is p-polarized light, and that is incident upon the dividing optical element 282 is reflected by a reflecting surface (not shown) in the second observation direction. The wavelength filter 302 removes light having wavelengths other than the second wavelength, and the polarizing filter 30P removes light other than the p-polarized light. The lens 322 collimates the light, and the image capturing surface of the second image capturing unit 142 is irradiated with the collimated light.
The object light that has the second wavelength and that is p-polarized light, and that is incident upon the second image capturing unit 142 and the reference light that has the second wavelength and that is p-polarized light, and that is incident upon the second image capturing unit 142 interfere with each other to generate an interference fringe at the image capturing surface of the second image capturing unit 142. The second image capturing unit 142 captures, as a second hologram, the interference fringe generated at the image capturing surface.
In the second exemplary embodiment, as in the first exemplary embodiment, the operation of the first unit 100A1 as a first hologram generating unit and the operation of the second unit 100A2 as a second hologram generating unit are performed at the same time. As a result, a region including observation regions that are observable at the same time is enlarged. In the second exemplary embodiment, as in the first exemplary embodiment, since the object light illuminates the observation object as a plane wave, a wide range of the observation object is uniformly irradiated. Therefore, the brightness of reconstructed images also becomes uniform.
In particular, in the second exemplary embodiment, the light having the first wavelength and the light having the second wavelength not only have different wavelengths but also have different polarization directions. Therefore, the “object light that has the first wavelength and that is s-polarized light” derived from the “light that has the first wavelength and that is s-polarized light” and the “object light that has the second wavelength and that is p-polarized light” derived from the “light that has the second wavelength and that is p-polarized light” are separated from each other by, for example, the wavelength filters and the polarizing filters. Therefore, by allowing the light to be transmitted through the polarizing filters in addition to through the wavelength filters, noise that is not capable of being completely removed by the wavelength filters is removed.
The hologram generating unit 12 according to the third exemplary embodiment includes a first unit 100B1 and a second unit 100B2. In the first unit 100B1, a dividing optical element 241 and a reflecting mirror 341 are not included, and a lens 231 is added. In addition, a light source 221, the lens 231, a dividing optical element 281, and a lens 381, are disposed in that order with their optical axes coinciding with each other such that an optical axis of an optical system thereof is orthogonal to an optical axis of object light. Further, a reflecting mirror 361 is disposed such that a reflecting surface is orthogonal to the optical axis of the optical system to reflect incident light in the direction of the lens 381.
In the second unit 100B2, a dividing optical element 242 and a reflecting mirror 342 are not included, and a lens 232 is added. In addition, a light source 222, the lens 232, a dividing optical element 282, and a lens 382, are disposed in that order with their optical axes coinciding with each other such that an optical axis of an optical system thereof is orthogonal to an optical axis of object light. Further, a reflecting mirror 362 is disposed such that a reflecting surface is orthogonal to the optical axis of the optical system to reflect incident light in the direction of the lens 382.
In the third exemplary embodiment, as in the first exemplary embodiment, a first observation direction and a second observation region intersect each other, and an optical axis of object light having a first wavelength and an optical axis of object light having a second wavelength also intersect each other. In the third exemplary embodiment, as in the first exemplary embodiment, the first unit 100B1 and the second unit 100B2 are disposed such that a focal position of a lens 262 differs from a focal position of a lens 261.
In the third exemplary embodiment, the optical components of the first unit 100B are disposed together in one place in the first observation direction with respect to the position of an observation object S, and the optical components of the second unit 100B2 are disposed together in one place in the second observation direction with respect to the position of the observation object S. This reduces the size of the entire digital holographic apparatus 10.
Next, the operation of the hologram generating unit 12 is described.
First, the operation of the first unit 100B1 is described. As shown in
The light (object light) having the first wavelength and reflected by the dividing optical element 281 is collimated by the lens 261 and illuminates the observation object S. That is, the light having the first wavelength and illuminating the observation object S is a “plane wave”. The light that illuminates the observation object S is scattered when it is reflected by the observation object S.
The object light having the first wavelength and reflected by the observation object S is collimated by the lens 261, and is incident upon the dividing optical element 281. The object light having the first wavelength and incident upon the dividing optical element 281 is transmitted through the reflecting surface 291 and exits therefrom in the first observation direction. The wavelength filter 301 removes light having wavelengths other than the first wavelength, and the lens 321 gathers the light to irradiate an image capturing surface of a first image capturing unit 141 with the gathered light.
The light (reference light) having the first wavelength and transmitted through the dividing optical element 281 illuminates the reflecting mirror 361, is collimated by the lens 381, illuminates the reflecting mirror 361, is reflected by the reflecting mirror 361 in the direction of the lens 381, is gathered by the lens 381, and is incident upon the dividing optical element 281.
The reference light having the first wavelength and incident upon the dividing optical element 281 is reflected by the reflecting surface 291 in the first observation direction. The wavelength filter 301 removes light having wavelengths other than the first wavelength, and the lens 321 collimates the light to irradiate the image capturing surface of the first image capturing unit 141 with the collimated light.
The object light having the first wavelength and illuminating the image capturing surface of the first image capturing unit 141 and the reference light having the first wavelength and illuminating the image capturing surface of the first image capturing unit 141 interfere with each other to generate an interference fringe at the image capturing surface of the first image capturing unit 141. The first image capturing unit 141 captures, as the first hologram, the interference fringe generated at the image capturing surface.
In the third exemplary embodiment, as in the first exemplary embodiment, the first observation direction and the second observation region intersect each other, and the optical axis of the object light having the first wavelength and the optical axis of the object light having the second wavelength also intersect each other. In the third exemplary embodiment, as in the first exemplary embodiment, the first unit 100B1 and the second unit 100B2 are disposed such that the focal position of the lens 262 differs from the focal position of the lens 261.
Next, the operation of the second unit 100B2 is described. As shown in
The light (object light) having the second wavelength and reflected by the dividing optical element 282 is collimated by the lens 262 and illuminates the observation object S. That is, the light having the second wavelength and illuminating the observation object S is a “plane wave”. The light that illuminates the observation object S is scattered when it is reflected by the observation object S.
The object light having the second wavelength and reflected by the observation object S is collimated by the lens 262, and is incident upon the dividing optical element 282. The object light having the second wavelength and incident upon the dividing optical element 282 is transmitted through the reflecting surface 292 and exits therefrom in the second observation direction. A wavelength filter 302 removes light having wavelengths other than the second wavelength, and a lens 322 gathers the light to irradiate an image capturing surface of a second image capturing unit 142 with the gathered light.
The light (reference light) having the second wavelength and transmitted through the dividing optical element 282 is collimated by the lens 382, illuminates the reflecting mirror 362, is reflected by the reflecting mirror 362 in the direction of the lens 382, is gathered by the lens 382, and is incident upon the dividing optical element 282.
The reference light having the second wavelength and incident upon the dividing optical element 282 is reflected by the reflecting surface 292 in the second observation direction. The wavelength filter 302 removes light having wavelengths other than the second wavelength, and the lens 322 collimates the light to irradiate the image capturing surface of the second image capturing unit 142 with the collimated light.
The object light having the second wavelength and illuminating the image capturing surface of the second image capturing unit 142 and the reference light having the second wavelength and illuminating the image capturing surface of the second image capturing unit 142 interfere with each other to generate an interference fringe at the image capturing surface of the second image capturing unit 142. The second image capturing unit 142 captures, as the second hologram, the interference fringe generated at the image capturing surface.
In the third exemplary embodiment, as in the first exemplary embodiment, the operation of the first unit 100B1 as the first hologram generating unit and the operation of the second unit 100B2 as the second hologram generating unit are performed at the same time. As a result, a region including observation regions that are observable at the same time is enlarged. In the third exemplary embodiment, as in the first exemplary embodiment, since the object light illuminates the observation object as a plane wave, a wide range of the observation object is uniformly irradiated. Therefore, the brightness of reconstructed images also becomes uniform.
In particular, in the third exemplary embodiment, since the reflection light reflected by the observation object is defined as the object light, a bright image (for example, having a high SN ratio) is obtained compared to the case in which the transmission light is defined as the object light. This is because, since the object light is generated at the surface of the observation object, the SN ratio of the object light is not reduced by absorption and scattering of the light in the observation object unlike the case in which the transmission light is defined as the object light.
The hologram generating unit 12 according to the fourth exemplary embodiment includes a first unit 100C1 and a second unit 100C2. Except that, in the first unit 100C1, a first light source 22C1 that projects “light having a first wavelength and being s-polarized light” is disposed in place of the first light source 221 that projects light having the first wavelength, and a polarizing filter 30S that transmits only s-polarized light is inserted between a wavelength filter 301 and a lens 321, the first unit 100C1 has the same structural features as the first unit 100B1 shown in
Except that, in the second unit 100C2, a second light source 22C2 that projects “light having a second wavelength and being p-polarized light” is used in place of the second light source 222 that projects light having the second wavelength, and a polarizing filter 30P that transmits only p-polarized light is inserted between a wavelength filter 302 and a lens 322, the second unit 100C2 has the same structural features as the second unit 100B2 shown in
In the fourth exemplary embodiment, as in the first exemplary embodiment, a first observation direction and a second observation region intersect each other, and an optical axis of object light having the first wavelength and an optical axis of object light having the second wavelength also intersect each other. In the fourth exemplary embodiment, as in the first exemplary embodiment, the first unit 100C1 and the second unit 100C2 are disposed such that a focal position of a lens 262 differs from a focal position of a lens 261.
Except that the “light having the first wavelength and being s-polarized light” is used in place of the light having the first wavelength, and the “light having the second wavelength and being p-polarized light” is used in place of the light having the second wavelength, the operation of the hologram generating unit according to the fourth exemplary embodiment is substantially the same as the operation of the hologram generating unit according to the third exemplary embodiment. Therefore, only differences are described.
In the first unit 100C1, object light that has the first wavelength and is s-polarized light, and that is incident upon a dividing optical element 281 is transmitted through a reflecting surface 291, and exits therefrom in the first observation direction. Then, the wavelength filter 301 removes light having wavelengths other than the first wavelength, and the polarizing filter 30S removes light other than the s-polarized light. Then, the lens 321 gathers the light, and an image capturing surface of a first image capturing unit 141 is irradiated with the gathered light.
On the other hand, reference light that has the first wavelength and that is s-polarized light, and that is incident upon the dividing optical element 281 is reflected by the reflecting surface 291 in the first observation direction. Then, the wavelength filter 301 removes light having wavelengths other than the first wavelength, and the polarizing filter 30S removes light other than the s-polarized light. Then, the lens 321 collimates the light, and the image capturing surface of the first image capturing unit 141 is irradiated with the collimated light.
The object light that has the first wavelength and that is s-polarized light, and that is incident upon the image capturing surface of the first image capturing unit 141 and the reference light that has the first wavelength and that is s-polarized light, and that is incident upon the image capturing surface of the first image capturing unit 141 interfere with each other to generate an interference fringe at the image capturing surface of the first image capturing unit 141. The first image capturing unit 141 captures, as a first hologram, the interference fringe generated at the image capturing surface.
Next, in the second unit 100C2, object light that has the second wavelength and that is p-polarized light, and that is incident upon a dividing optical element 282 is transmitted through a reflecting surface 292 and exits therefrom in the second observation direction. Then, the wavelength filter 302 removes light having wavelengths other than the second wavelength, and the polarizing filter 30P removes light other than the p-polarized light. The lens 322 gathers the light, and an image capturing surface of a second image capturing unit 142 is irradiated with the gathered light.
On the other hand, reference light that has the second wavelength and that is p-polarized light, and that is incident upon the dividing optical element 282 is reflected by a reflecting surface (not shown) in the second observation direction. The wavelength filter 302 removes light having wavelengths other than the second wavelength, and the polarizing filter 30P removes light other than the p-polarized light. The lens 322 collimates the light, and the image capturing surface of the second image capturing unit 142 is irradiated with the collimated light.
The object light that has the second wavelength and that is p-polarized light, and that is incident upon the second image capturing unit 142 and the reference light that has the second wavelength and that is p-polarized light, and that is incident upon the second image capturing unit 142 interfere with each other to generate an interference fringe at the image capturing surface of the second image capturing unit 142. The second image capturing unit 142 captures, as a second hologram, the interference fringe generated at the image capturing surface.
In the fourth exemplary embodiment, as in the first exemplary embodiment, the operation of the first unit 100C1 as a first hologram generating unit and the operation of the second unit 100C2 as a second hologram generating unit are performed at the same time. As a result, a region including observation regions that are observable at the same time is enlarged. In the fourth exemplary embodiment, as in the first exemplary embodiment, since the object light illuminates the observation object as a plane wave, a wide range of the observation object is uniformly irradiated. Therefore, the brightness of reconstructed images also becomes uniform.
In particular, in the fourth exemplary embodiment, as in the third exemplary embodiment, since the reflection light reflected by the observation object is defined as the object light, a bright image (for example, having a high SN ratio) is obtained compared to the case in which the transmission light is defined as the object light.
In particular, in the fourth exemplary embodiment, as in the second exemplary embodiment, the light having the first wavelength and the light having the second wavelength not only have different wavelengths but also have different polarization directions. Therefore, by allowing the light to be transmitted through the polarizing filters in addition to through the wavelength filters, noise that is not capable of being completely removed by the wavelength filters is removed.
The first unit 201 includes a first light source 221 that projects light having a first wavelength (λ1); a lens 241; a first dividing optical element 261 having a reflecting surface 271 that divides, combines, transmits, or reflects incident light in accordance with an incidence direction; a lens 281; a wavelength filter 301 that transmits only light having a second wavelength (λ2); a lens 321; a second image capturing unit 142; and a lens 361. The optical components are disposed on the same substrate. By disposing the second image capturing unit 142 (on an optical axis of the object light described below) in the first unit 201, the size of the entire digital holographic apparatus 10 is reduced.
The second unit 202 includes a second light source 222 that projects light having a second wavelength (λ2); a lens 242; a second dividing optical element 262 having a reflecting surface 272 that divides, combines, transmits, or reflects incident light in accordance with an incidence direction; a lens 282; a wavelength filter 302 that transmits only light having the first wavelength (λ1); a lens 322; a first image capturing unit 141; and a lens 362. The optical components are disposed on the same substrate. By disposing the first image capturing unit 141 (on an optical axis of the object light described below) in the second unit 202, the size of the entire digital holographic apparatus 10 is reduced.
As the first dividing optical element 261 and the second dividing optical element 262, a beam splitter or the like may be used. As the wavelength filter 301 and the wavelength filter 302, a bandpass filter or the like may be used.
In the first unit 201, other optical components are disposed in all directions with respect to the first dividing optical element 261. The lens 281 is disposed in a first direction with respect to the first dividing optical element 261. The wavelength filter 301, the lens 321, and the second image capturing unit 142 are disposed in a second direction with respect to the first dividing optical element 261. The lens 361 is disposed in a third direction with respect to the first dividing optical element 261. The first light source 221 and the lens 241 are disposed in a fourth direction with respect to the first dividing optical element 261. The fourth direction is a counter direction with respect to the third direction.
The lens 281, the first dividing optical element 261, the wavelength filter 301, and the lens 321 are disposed in that order along the second direction with their optical axes coinciding with each other. As described below, the optical axes of these optical systems are equivalent to an optical axis of object light having the second wavelength. The second image capturing unit 142 is disposed in the second direction with respect to the lens 321 such that its image surface intersects the optical axes of the optical systems (the optical axis of the object light having the second wavelength). The second image capturing unit 142 is disposed at a focal position of the lens 321.
The first light source 221, the lens 241, the first dividing optical element 261, and the lens 361 are disposed in that order along the third direction with their optical axes coinciding with each other. The reflecting mirror 381 is disposed so as to intersect the optical axes of these optical systems. In the illustrated example, the reflecting mirror 381 includes a reflecting surface that is inclined at an angle of 45 degrees with respect to the optical axes of the optical systems such that incident light is reflected in the direction of the reflecting mirror 382.
In the second unit 202, other optical components are disposed in all directions with respect to the second dividing optical element 262. The lens 282 is disposed in the second direction with respect to the second dividing optical element 262. The wavelength filter 302, the lens 322, and the first image capturing unit 141 are disposed in the first direction with respect to the second dividing optical element 262. The lens 362 is disposed in the third direction with respect to the second dividing optical element 262. The second light source 222 and the lens 242 are disposed in the fourth direction with respect to the second dividing optical element 262.
The lens 282, the second dividing optical element 262, the wavelength filter 302, and the lens 322 are disposed in that order along the first direction with their optical axes coinciding with each other. As described below, the optical axes of these optical systems are equivalent to an optical axis of object light having the first wavelength. The first image capturing unit 141 is disposed in the first direction with respect to the lens 322 such that its image surface intersects the optical axes of the optical systems (the optical axis of the object light having the first wavelength). The first image capturing unit 141 is disposed at a focal position of the lens 322.
The second light source 222, the lens 242, the second dividing optical element 262, and the lens 362 are disposed in that order along the third direction with their optical axes coinciding with each other. The reflecting mirror 382 is disposed so as to intersect the optical axes of these optical systems. In the illustrated example, the reflecting mirror 382 includes a reflecting surface that is inclined at an angle of 45 degrees with respect to the optical axes of the optical systems such that incident light is reflected in the direction of the reflecting mirror 381.
In the fifth exemplary embodiment, the optical axis of the object light having the first wavelength and the optical axis of the object light having the second wavelength are coaxial. Therefore, when these axes need not be distinguished, these axes are generally called “the optical axis of the object light”. That is, the lens 281, the first dividing optical element 261, the wavelength filter 301, and the lens 321 of the first unit 201, and the lens 282, the second dividing optical element 262, the wavelength filter 302, and the lens 322 of the second unit 202 are each disposed along the optical axis of the object light.
In the fifth exemplary embodiment, the first unit 201 and the reflecting mirror 381, and the second unit 202 and the reflecting mirror 382 are symmetrically disposed with respect to a plane including the position of an observation object S and being perpendicular to the optical axis of the object light. In the description below, this plane is referred to as a “reference plane including the position of the observation object”. The observation object S is held by a holding member (not shown). The first unit 201 and the second unit 202 are disposed such that a focal position of the lens 281 and a focal position of the lens 282 are disposed within the observation object S. As described below, the focal position of the lens 282 differs from the focal position of the lens 281.
Although the lens 241, the lens 281, the lens 362, and the lens 322 form a 4f optical system with respect to light having the first wavelength, the lens 282 and the lens 322 may form an infinity correction optical system with respect to the light having the first wavelength. Similarly, although the lens 242, the lens 282, the lens 361, and the lens 321 form a 4f optical system with respect to the light having the second wavelength, the lens 281 and the lens 321 may form an infinity correction optical system with respect to the light having the second wavelength. Although, in each 4f optical system, the focal lengths of the lenses are equal to each other, the focal lengths of the lenses in each infinity correction optical system may differ from each other.
Next, an operation of the hologram generating unit 12 is described.
The operation of the hologram generating unit 12 as the first hologram generating unit is described. As shown in
The light having the first wavelength and incident upon the first dividing optical element 261 is such that a part thereof is reflected by the reflecting surface 271 in the first direction. In addition, the light having the first wavelength and incident upon the first dividing optical element 261 is such that a remaining part thereof is transmitted through the reflecting surface 271 and exits therefrom in the third direction. This causes the light having the first wavelength and projected from the first light source 221 to be divided into object light and reference light. The reference light is indicated by dotted lines.
The light (object light) having the first wavelength and reflected by the first dividing optical element 261 in the first direction is collimated by the lens 281, and illuminates the observation object S. The light having the first wavelength and illuminating the observation object S is a “plane wave”. The light that illuminates the observation object S is scattered when it is transmitted through the observation object S.
The object light having the first wavelength and transmitted through the observation object S is collimated by the lens 282, and is incident upon the second dividing optical element 262. The object light having the first wavelength and incident upon the second dividing optical element 262 is transmitted through the reflecting surface 272 and exits therefrom in the first direction. The wavelength filter 302 removes light having wavelengths other than the first wavelength, and the lens 322 gathers the light to irradiate an image capturing surface of the first image capturing unit 141 with the gathered light.
The light (reference light) having the first wavelength and exiting from the first dividing optical element 261 in the third direction is collimated by the lens 361, and illuminates the reflecting mirror 381. The collimated reference light having the first wavelength is reflected by the reflecting mirror 381 in the direction of the reflecting mirror 382, is reflected by the reflecting mirror 382 in the direction of the lens 362, is gathered by the lens 362, and is incident upon the second dividing optical element 262.
The reference light having the first wavelength and incident upon the dividing optical element 262 is reflected by the reflecting surface 272 in the first direction. The wavelength filter 302 removes light having wavelengths other than the first wavelength, and the lens 322 collimates the light to irradiate the image capturing surface of the first image capturing unit 141 with the collimated light.
The object light having the first wavelength and illuminating the image capturing surface of the first image capturing unit 141 and the reference light having the first wavelength and illuminating the image capturing surface of the first image capturing unit 141 interfere with each other to generate an interference fringe at the image capturing surface of the first image capturing unit 141. The first image capturing unit 141 captures, as the first hologram, the interference fringe generated at the image capturing surface.
Next, the operation of the hologram generating unit as the second hologram generating unit is described. As shown in
The light having the second wavelength and incident upon the second dividing optical element 262 is such that a part thereof is reflected by the reflecting surface 272 in the second direction. In addition, the light having the second wavelength and incident upon the second dividing optical element 262 is such that a remaining part thereof is transmitted through the reflecting surface 272, and exits therefrom in the third direction. This causes the light having the second wavelength and projected from the second light source 222 to be divided into object light and reference light. The reference light is indicated by dotted lines.
The light (object light) having the second wavelength and reflected by the second dividing optical element 262 in the second direction is collimated by the lens 282, and illuminates the observation object S. That is, the light having the second wavelength and illuminating the observation object S is a “plane wave”. The light that illuminates the observation object S is scattered when it is transmitted through the observation object S.
The object light having the second wavelength and transmitted through the observation object S is collimated by the lens 281, and is incident upon the dividing optical element 261. The object light having the second wavelength and incident upon the first dividing optical element 261 is transmitted through the reflecting surface 271 and exits therefrom in the second direction. The wavelength filter 301 removes light having wavelengths other than the second wavelength, and the lens 321 gathers the light to irradiate an image capturing surface of the second image capturing unit 142 with the gathered light.
The light (reference light) having the second wavelength and exiting from the second dividing optical element 262 in the third direction is collimated by the lens 362, and illuminates the reflecting mirror 382. The collimated reference light having the second wavelength is reflected by the reflecting mirror 382 in the direction of the reflecting mirror 381, and is reflected by the reflecting mirror 381 in the direction of the lens 361. The lens 361 gathers the light, and the gathered light is incident upon the first dividing optical element 261.
The reference light having the second wavelength and incident upon the first dividing optical element 261 is reflected by the reflecting surface 271 in the second direction. The wavelength filter 301 removes light having wavelengths other than the second wavelength, and the lens 321 collimates the light to irradiate the image capturing surface of the second image capturing unit 142 with the collimated light.
The object light having the second wavelength and illuminating the image capturing surface of the second image capturing unit 142 and the reference light having the second wavelength and illuminating the image capturing surface of the second image capturing unit 142 interfere with each other to generate an interference fringe at the image capturing surface of the second image capturing unit 142. The second image capturing unit 141 captures, as the second hologram, the interference fringe generated at the image capturing surface.
In the fifth exemplary embodiment, the optical axis of the object light having the first wavelength and the optical axis of the object light having the second wavelength are coaxial. By disposing the optical components symmetrically with respect to the reference plane including the position of the observation object, the optical components and optical paths are made common, so that the operation of the hologram generating unit 12 as the first hologram generating unit and the operation of the hologram generating unit 12 as the second hologram generating unit are performed at the same time. This enlarges a region including observation regions that are observed at the same time.
Since the wavelength of the light having the first wavelength and the wavelength of the light having the second wavelength differ from each other, it is possible to irradiate the observation object with the light having the first wavelength and the light having the second wavelength to generate the object light having the first wavelength and the object light having the second wavelength. The generated object light having the first wavelength and the generated object light having the second wavelength are separated by, for example, the wavelength filter.
In the fifth exemplary embodiment, since the object light illuminates the observation object as a plane wave, a wide range of the observation object is uniformly irradiated. Therefore, the brightness of reconstructed images also becomes uniform. For example, when the inside of a plane of the observation object or a wide range in a depth direction of the observation object is to be photographed, a method of irradiating the observation object with the object light as a plane wave is a suitable method.
In the fifth exemplary embodiment, the optical axis of the object light and the optical axis of the reference light having the same wavelength are coaxial with respect to each other (in-line relationship). However, the optical axis of the reference light may be inclined such that the optical axis of the object light and the optical axis of the reference light intersect each other (off-axis relationship). The off-axis relationship makes it easier for first-order diffracted light and zeroth order diffracted light to be separated, and allows noise that is generated at the reconstructed images by the zeroth order diffracted light to be reduced.
For example, in order to obliquely irradiate the observation object with the reference light having the first wavelength, the second dividing optical element 262 only needs to be inclined. Alternatively, a deflecting element that changes the optical path of the reference light only needs to be inserted in front of or in back of the wavelength filter 302. The deflecting element may be, for example, an optical element such as a wedge type prism.
Enlargement of Region that is Observed
Next, the enlargement of a region that is observed is described.
Therefore, as shown in
Here, the term “focal depth” refers to the “distance from where a focal position is located on an image capturing element in the direction of the optical axis” at which blurring of an optical image formed when the object light is focused is permitted. In addition, the term “depth of field” refers to the “distance from where a focal position in the observation object in the direction of the optical axis (that is, the distance of reconstruction from a hologram” at which blurring of an optical image formed when the object light is focused is permitted.
That is, the range that is indicated by a thick solid double-headed arrow and that extends between a location in front of and a location in back of the focal position 1 in the direction of the optical axis corresponds to a depth of field (λ1) based on the object light having the first wavelength. In addition, the range that is indicated by a thick dotted-line double-headed arrow and that extends between a location in front of and a location in back of the focal position 2 corresponds to a depth of field (λ2) based on the object light having the second wavelength.
As shown in
Since the focal position 1 and the focal position 2 are displaced from each other in the direction of the optical axis of the object light, the depth of field (λ1) and the depth of field (λ2) are displaced from each other in the direction of the optical axis, and the first observation region (λ1) and the second observation region (λ2) are also displaced from each other in the direction of the optical axis. Therefore, a depth of field is increased by combining the depth of field (λ1) and the depth of field (λ2) with each other. Consequently, compared to the case in which the observation object is irradiated with light having a single wavelength, a region including observation regions that are observed at the same time is enlarged to a region in which the first observation region (λ1) and the second observation region (λ2) are connected to each other.
When the wavelength is X, the numerical aperture of the lenses 281 and 282, which are objective lenses, is NA, the diameter of pixels of the image capturing units (that is, the pixel pitch) is p, the number of pixels of the image capturing units is N, and the magnification is M, the depth of field is a range from the focal position and is expressed by the following Formula (1). In Formula (1) below, “N” of the numerator represents the number of pixels:
Formula (1) above indicates the range that allows the resolving power that is determined by the NA of the objective lenses to be maintained. Therefore, since the required resolution depends upon use, formulas for the depth of field are not limited to this formula. The image information processing unit 16 connects the first observation region (λ1) and the second observation region (λ2) to each other (see
The image information processing unit 16 reads out image information about the first hologram and image information about the second hologram that are associated with each other from the storage device, processes the image information about the first hologram to generate a first reconstructed image, and processes the image information about the second hologram to generate a second reconstructed image. By combining the first reconstructed image and the second reconstructed image, the first observation region (λ1) and the second observation region (λ2) are connected to each other. An image in which the observation regions are connected to each other, that is, a composite image of the first reconstructed image and the second reconstructed image is displayed on the display 18.
In associating the image information about the first hologram and the image information about the second hologram with each other and in storing them, the three-dimensional coordinates of the focal position 1 where the object light having the first wavelength is focused and the three-dimensional coordinates of the focal position 2 where the object light having the second wavelength is focused may be stored together. For example, when the holding member that holds the observation object S includes a position sensor or the like, the three-dimensional coordinates of the focal positions of the lenses 281 and 282, which are objective lenses, are determined. The three-dimensional coordinates of the focal position 1 and the three-dimensional coordinates of the focal position 2 are used when combining the first reconstructed image and the second reconstructed image.
In the example shown in
Here, a mode of enlargement of a region that is observed is described.
(1) Direction of Displacement of Region that is Observed
Although
As shown in
Therefore, as shown in
(2) State of Overlap of Region that is Observed
The state of overlap of the two observation regions is not limited to that mentioned above. For example, as shown in
As shown in
(3) Magnification of Observation Regions
In the example shown in
As shown in
The hologram generating unit 12 according to the sixth exemplary embodiment includes a lens 40 and a pair of masks 421 and 422, in addition to a first unit 20A1, a second unit 20A2, and a pair of reflecting mirrors 381 and 382. The first unit 20A1 has the same structure as the first unit 201 shown in
The second unit 20A2 has the same structure as the second unit 202 shown in
In the sixth exemplary embodiment, the first unit 20A1 and the reflecting mirror 381 and the second unit 20A2 and the reflecting mirror 382 are symmetrically disposed with respect to a reference plane including the position of an observation object S. The lens 40 is disposed in the reference plane. The masks 421 and 422 are symmetrically disposed with respect to the reference plane including the position of the observation object S.
In the sixth exemplary embodiment, as in the fifth exemplary embodiment, multiple optical components are disposed along an optical axis of object light such that an optical axis of object light having a first wavelength is coaxial with an optical axis of object light having a second wavelength. Further, in the sixth exemplary embodiment, as in the fifth exemplary embodiment, the first unit 20A1 and the second unit 20A2 are disposed such that a focal position of a lens 282 differs from a focal position of a lens 281.
Next, an operation of the hologram generating unit 12 is described.
First, the operation of the hologram generating unit as the first hologram generating unit is described. As shown in
The light (object light) having the first wavelength and reflected in the first direction by the first dividing optical element 261 is gathered by a lens 281 and illuminates an observation object S. That is, the light having the first wavelength and illuminating the observation object S is “convergent light”. The light that illuminates the observation object S is scattered when it is transmitted through the observation object S.
The object light having the first wavelength and transmitted through the observation object S is collimated by a lens 282, and is incident upon a second dividing optical element 262. The object light having the first wavelength and incident upon a second dividing optical element 262 is transmitted through a reflecting surface 272 and exits therefrom in the first direction. A wavelength filter 302 removes light having wavelengths other than the first wavelength, and a lens 322 gathers the light to irradiate an image capturing surface of a first image capturing unit 141 with the gathered light.
The light (reference light) having the first wavelength and exiting from the first dividing optical element 261 in the third direction is gathered by a lens 361 and illuminates the reflecting mirror 381. The gathered reference light having the first wavelength is reflected by the reflecting mirror 381 in the direction of the reflecting mirror 382, is formed into a predetermined shape (such as a rectangular shape) by the mask 421, and is collimated by the lens 40. The reference light collimated by the lens 40 is reflected by the reflecting mirror 382 in the direction of a lens 362, is gathered by the lens 362, and is incident upon the second dividing optical element 262.
The reference light having the first wavelength and incident upon the second dividing optical element 262 is reflected by the reflecting surface 272 in the first direction. The wavelength filter 302 removes light having wavelengths other than the first wavelength, and the lens 322 collimates the light to irradiate the image capturing surface of the first image capturing unit 141 with the collimated light. The first image capturing unit 141 captures, as the first hologram, the interference fringe generated at the image capturing surface.
Next, the operation of the hologram generating unit as the second hologram generating unit is described. As shown in
The light (object light) having the second wavelength and reflected in the second direction by the second dividing optical element 262 is gathered by the lens 282 and illuminates the observation object S. That is, the light having the second wavelength and illuminating the observation object S is “convergent light”. The light that illuminates the observation object S is scattered when it is transmitted through the observation object S.
The object light having the second wavelength and transmitted through the observation object S is collimated by the lens 281, and is incident upon the first dividing optical element 261. The object light having the second wavelength and incident upon the first dividing optical element 261 is transmitted through the reflecting surface 271 and exits therefrom in the second direction. The wavelength filter 301 removes light having wavelengths other than the second wavelength, and the lens 321 gathers the light to irradiate an image capturing surface of a second image capturing unit 142 with the gathered light.
The light (reference light) having the second wavelength and exiting from the second dividing optical element 262 in the third direction is gathered by the lens 362 and illuminates the reflecting mirror 382. The gathered reference light having the second wavelength is reflected by the reflecting mirror 382 in the direction of the reflecting mirror 381, is formed into a predetermined shape (such as a rectangular shape) by the mask 422, and is collimated by the lens 40. The reference light collimated by the lens 40 is reflected by the reflecting mirror 381 in the direction of the lens 361, is gathered by the lens 361, and is incident upon the first dividing optical element 261.
The reference light having the second wavelength and incident upon the first dividing optical element 261 is reflected by the reflecting surface 271 in the second direction. The wavelength filter 301 removes light having wavelengths other than the second wavelength, and the lens 321 collimates the light to irradiate the image capturing surface of the second image capturing unit 142 with the collimated light. The object light having the second wavelength and illuminating the image capturing surface of the second image capturing unit 142 and the reference light having the second wavelength and illuminating the image capturing surface of the second image capturing unit 142 interfere with each other to generate an interference fringe at the image capturing surface of the second image capturing unit 142. The second image capturing unit 142 captures, as the second hologram, the interference fringe generated at the image capturing surface.
In the sixth exemplary embodiment, as in the fifth exemplary embodiment, the optical axis of the object light having the first wavelength and the optical axis of the object light having the second wavelength are coaxial. By disposing the optical components symmetrically with respect to the reference plane including the position of the observation object, the optical components and optical paths are made common, so that the operation of the hologram generating unit 12 as the first hologram generating unit and the operation of the hologram generating unit 12 as the second hologram generating unit are performed at the same time. This enlarges a region including observation regions that are observed at the same time compared to the case in which the observation object is irradiated with light having a single wavelength and is observed.
In the sixth exemplary embodiment, the observation object is irradiated with object light that is convergent light. Therefore, compared to the case in which the observation object is irradiated with object light that is a plane wave, a region that is observed is narrow. However, since the light is concentrated in a narrow region, a bright image (for example, having a high SN ratio) is obtained. The method of irradiating the observation object with object light that is convergent light is suitable to, for example, the case in which the observation object is one that considerably absorbs or scatters light.
The hologram generating unit 12 according to the seventh exemplary embodiment includes a first unit 20B1 and a second unit 20B2. Except that, in the first unit 20B1, a wavelength filter 30B1 that transmits only light having a first wavelength (λ1) is disposed in place of the wavelength filter 301 that transmits only light having the second wavelength (λ2), a first image capturing unit 141 is disposed in place of the second image capturing unit 142, and a reflecting mirror 381 is included in the first unit 20B1, the first unit 20B1 has the same structure as the first unit 201 shown in
A first light source 221, a lens 241, a first dividing optical element 261, and a lens 361 are disposed in that order in a third direction with their optical axes coinciding with each other. The reflecting mirror 381 is disposed such that a reflecting surface is orthogonal to optical axes of these optical systems so that incident light is reflected in the direction of the lens 361.
Except that, in the second unit 20B2, a wavelength filter 30B2 that transmits only light having a second wavelength (λ2) is disposed in place of the wavelength filter 302 that transmits only light having the first wavelength (λ1), a second image capturing unit 142 is disposed in place of the first image capturing unit 141, and a reflecting mirror 382 is included in the second unit 20B2, the second unit 20B2 has the same structure as the second unit 202 shown in
A second light source 222, a lens 242, a second dividing optical element 262, and a lens 362 are disposed in that order in the third direction with their optical axes coinciding with each other. The reflecting mirror 382 is disposed such that a reflecting surface is orthogonal to optical axes of these optical systems so that incident light is reflected in the direction of the lens 362.
In the seventh exemplary embodiment, the first unit 20B1 and the second unit 20B2 are symmetrically disposed with respect to a reference plane including the position of an observation object S. In the seventh exemplary embodiment, as in the fifth exemplary embodiment, the optical components are disposed along an optical axis of object light such that an optical axis of object light having the first wavelength is coaxial with an optical axis of object light having the second wavelength. Further, in the seventh exemplary embodiment, the first unit 20B1 and the second unit 20B2 are disposed such that a focal position of a lens 282 differs from a focal position of a lens 281.
Next, the operation of the hologram generating unit 12 is described.
First, the operation of the hologram generating unit as the first hologram generating unit is described. As shown in
The light having the first wavelength and incident upon the first dividing optical element 261 is such that a part thereof is reflected by a reflecting surface 271 in a first direction. In addition, the light having the first wavelength and incident upon the first dividing optical element 261 is such that a remaining part thereof is transmitted through the reflecting surface 271 and exits therefrom in the third direction. This causes the light having the first wavelength and projected from the first light source 221 to be divided into object light and reference light. The reference light is indicated by dotted lines.
The light (object light) having the first wavelength and reflected by the first dividing optical element 261 in the first direction is collimated by the lens 281 and illuminates an observation object S. The light having the first wavelength and illuminating the observation object S is a “plane wave”. The light that illuminates the observation object S is scattered when it is reflected by the observation object S.
As indicated by the alternate long and short dash lines, the object light having the first wavelength and reflected by the observation object S is collimated by the lens 281, and is incident upon the first dividing optical element 261. The object light having the first wavelength and incident upon the first dividing optical element 261 is transmitted through the reflecting surface 271 and exits therefrom in a second direction. The wavelength filter 30B1 removes light having wavelengths other than the first wavelength, and a lens 321 gathers the light to irradiate an image capturing surface of the first image capturing unit 141 with the gathered light.
The light (reference light) having the first wavelength and exiting from the first dividing optical element 261 in the third direction is collimated by the lens 361, and illuminates the reflecting mirror 381. The collimated reference light having the first wavelength is reflected by the reflecting mirror 381 in the direction of the lens 361, is gathered by the lens 361, and is incident upon the first dividing optical element 261.
The reference light having the first wavelength and incident upon the first dividing optical element 261 is reflected by the reflecting surface 271 in the second direction. The wavelength filter 30B1 removes light having wavelengths other than the first wavelength, and the lens 321 collimates the light to irradiate the image capturing surface of the first image capturing unit 141 with the collimated light.
The object light having the first wavelength and illuminating the image capturing surface of the first image capturing unit 141 and the reference light having the first wavelength and illuminating the image capturing surface of the first image capturing unit 141 interfere with each other to generate an interference fringe at the image capturing surface of the first image capturing unit 141. The first image capturing unit 141 captures, as the first hologram, the interference fringe generated at the image capturing surface.
Next, the operation of the hologram generating unit as the second hologram generating unit is described. As shown in
The light having the second wavelength and incident upon the second dividing optical element 262 is such that a part thereof is reflected by a reflecting surface 272 in the second direction. In addition, the light having the second wavelength and incident upon the second dividing optical element 262 is such that a remaining part thereof is transmitted through the reflecting surface 272 and exits therefrom in the third direction. This causes the light having the second wavelength and projected from the second light source 222 to be divided into object light and reference light. The reference light is indicated by dotted lines.
The light (object light) having the second wavelength and reflected by the second dividing optical element 262 in the second direction is collimated by the lens 282 and illuminates the observation object S. That is, the light having the second wavelength and illuminating the observation object S is a “plane wave”. The light that illuminates the observation object S is scattered when it is reflected by the observation object S.
As indicated by the alternate long and short dash lines, the object light having the second wavelength and reflected by the observation object S is collimated by the lens 282, and is incident upon the second dividing optical element 262. The object light having the second wavelength and incident upon the second dividing optical element 262 is transmitted through the reflecting surface 272 and exits therefrom in the first direction. A wavelength filter 30B2 removes light having wavelengths other than the second wavelength, and a lens 322 gathers the light to irradiate an image capturing surface of a second image capturing unit 142 with the gathered light.
The light (reference light) having the second wavelength and exiting from the second dividing optical element 262 in the third direction is collimated by the lens 362, and illuminates the reflecting mirror 382. The collimated reference light having the second wavelength is reflected by the reflecting mirror 382 in the direction of the lens 362, is gathered by the lens 362, and is incident upon the second dividing optical element 262.
The reference light having the second wavelength and incident upon the second dividing optical element 262 is reflected by the reflecting surface 272 in the first direction. The wavelength filter 30B2 removes light having wavelengths other than the second wavelength, and the lens 322 collimates the light to irradiate the image capturing surface of the second image capturing unit 142 with the collimated light.
The object light having the second wavelength and illuminating the image capturing surface of the second image capturing unit 142 and the reference light having the second wavelength and illuminating the image capturing surface of the second image capturing unit 142 interfere with each other to generate an interference fringe at the image capturing surface of the second image capturing unit 142. The second image capturing unit 142 captures, as the second hologram, the interference fringe generated at the image capturing surface.
In the seventh exemplary embodiment, the optical axis of the object light having the first wavelength and the optical axis of the object light having the second wavelength are coaxial. By disposing the optical components symmetrically with respect to the reference plane including the position of the observation object, the operation of the hologram generating unit 12 as the first hologram generating unit and the operation of the hologram generating unit 12 as the second hologram generating unit are performed at the same time. This enlarges a region including observation regions that are observed at the same time compared to the case in which the observation object is irradiated with light having a single wavelength and is observed.
In the seventh exemplary embodiment, since the reflection light reflected by the observation object is defined as the object light, a bright image (for example, having a high SN ratio) is obtained compared to the case in which the transmission light is defined as the object light. This is because, since the object light is generated at the surface of the observation object, the SN ratio of the object light is not reduced by absorption and scattering of the light in the observation object unlike the case in which the transmission light is defined as the object light. Due to the same reason, when the reflection light reflected by the observation object is the object light, the object light need not be “convergent light”.
The hologram generating unit 12 according to the eighth exemplary embodiment includes a first unit 20C1, a second unit 20C2, and a pair of reflecting mirrors 381 and 382. Except that, in the first unit 20C1, a first light source 22C1 that projects “light having a first wavelength and being s-polarized light” is disposed in place of the first light source 221 that projects light having the first wavelength, and a polarizing filter 30P that transmits only p-polarized light is inserted between a wavelength filter 301 and a lens 321, the first unit 20C1 has the same structural features as the first unit 201 shown in
Except that, in the second unit 20C2, a second light source 22C2 that projects “light having a second wavelength and being p-polarized light” is disposed in place of the second light source 222 that projects light having the second wavelength, and a polarizing filter 30S that transmits only s-polarized light is inserted between a wavelength filter 302 and a lens 322, the second unit 20C2 has the same structural features as the second unit 202 shown in
In the eighth exemplary embodiment, the first unit 20C1 and the reflecting mirror 381 and the second unit 20C2 and the reflecting mirror 382 are symmetrically disposed with respect to a reference plane including the position of an observation object S. In the eighth exemplary embodiment, as in the fifth exemplary embodiment, the optical components are disposed along an optical axis of object light such that an optical axis of object light having the first wavelength is coaxial with an optical axis of object light having the second wavelength. Further, in the eighth exemplary embodiment, as in the fifth exemplary embodiment, the first unit 20C1 and the second unit 20C2 are disposed such that a focal position of a lens 282 differs from a focal length of a lens 281.
Except that the “light having the first wavelength and being s-polarized light” is used in place of the light having the first wavelength, and the “light having the second wavelength and being p-polarized light” is used in place of the light having the second wavelength, the operation of the hologram generating unit according to the eighth exemplary embodiment is substantially the same as the operation of the hologram generating unit according to the fifth exemplary embodiment. Therefore, only differences are described.
Object light that has the first wavelength and is s-polarized light, and that exits from a second dividing optical element 262 in a first direction is such that the wavelength filter 302 removes light having wavelengths other than the first wavelength, and the polarizing filter 30S removes light other than the s-polarized light. Then, the lens 322 gathers the light, and an image capturing surface of a first image capturing unit 141 is irradiated with the gathered light.
Reference light that has the first wavelength and is s-polarized light and that exits from the second dividing optical element 262 in the first direction is such that the wavelength filter 302 removes light having wavelengths other than the first wavelength, and the polarizing filter 30S removes light other than the s-polarized light. Then, the lens 322 collimates the light, and the image capturing surface of the first image capturing unit 141 is irradiated with the collimated light.
The object light that has the first wavelength and is s-polarized light, and that illuminates the image capturing surface of the first image capturing unit 14, and the reference light that has the first wavelength and that is s-polarized light, and that illuminates the image capturing surface of the first image capturing unit 141 interfere with each other to generate an interference fringe at the image capturing surface of the first image capturing unit 141. The first image capturing unit 141 captures, as a first hologram, the interference fringe generated at the image capturing surface.
Object light that has the second wavelength and is p-polarized light, and that exits from a first dividing optical element 261 in a second direction is such that the wavelength filter 301 removes light having wavelengths other than the second wavelength, and the polarizing filter 30P removes light other than the p-polarized light. Then, the lens 321 gathers the light, and an image capturing surface of a second image capturing unit 142 is irradiated with the gathered light.
Reference light that has the second wavelength and is p-polarized light and that exits from the first dividing optical element 261 in the second direction is such that the wavelength filter 301 removes light having wavelengths other than the second wavelength, and the polarizing filter 30S removes light other than the p-polarized light. Then, the lens 321 collimates the light, and the image capturing surface of the second image capturing unit 142 is irradiated with the collimated light.
The object light that has the second wavelength and is p-polarized light, and that illuminates the image capturing surface of the second image capturing unit 142, and the reference light that has the second wavelength and that is p-polarized light, and that illuminates the image capturing surface of the second image capturing unit 142 interfere with each other to generate an interference fringe at the image capturing surface of the second image capturing unit 142. The second image capturing unit 142 captures, as a second hologram, the interference fringe generated at the image capturing surface.
In the eighth exemplary embodiment, the optical axis of the object light having the first wavelength and being s-polarized light and the optical axis of the object light having the second wavelength and being p-polarized light are coaxial. By disposing the optical components symmetrically with respect to the reference plane including the position of the observation object, the optical components and optical paths are made common, so that the operation of the hologram generating unit 12 as a first hologram generating unit and the operation of the hologram generating unit 12 as a second hologram generating unit are performed at the same time. This enlarges a region including observation regions that are observed at the same time compared to the case in which the observation object is irradiated with light having a single wavelength and is observed.
Since the wavelength of the light having the first wavelength and the wavelength of the light having the second wavelength differ from each other, and the polarization direction of the s-polarized light and the polarization direction of the p-polarized light differ from each other, it is possible to irradiate the observation object with the light having the first wavelength and being s-polarized light and the light having the second wavelength and being p-polarized light to generate the “object light having the first wavelength and being s-polarized light” and the “object light having the second wavelength and being p-polarized light”. The generated “object light having the first wavelength and being s-polarized light” and the generated “object light having the second wavelength and being p-polarized light” are separated by, for example, the wavelength filters and the polarizing filters. Therefore, by allowing the light to be transmitted through the polarizing filters in addition to through the wavelength filters, noise that is not capable of being completely removed by the wavelength filters is removed.
The hologram generating unit 12 according to the ninth exemplary embodiment includes a first unit 20D1 and a second unit 20D2. Except that, in the first unit 20D1, a first light source 22D1 that projects “light having a first wavelength and being s-polarized light” is disposed in place of the first light source 221 that projects the light having the first wavelength, and a polarizing filter 30S that transmits only s-polarized light is inserted between a wavelength filter 301 and a lens 321, the first unit 20D1 has the same structure as the first unit 20B1 shown in
Except that, in the second unit 20D2, a second light source 22D2 that projects “light having a second wavelength and being p-polarized light” is disposed in place of the second light source 222 that projects the light having the second wavelength, and a polarizing filter 30P that transmits only p-polarized light is inserted between a wavelength filter 302 and a lens 322, the second unit 20D2 has the same structure as the second unit 20B2 shown in
In the ninth exemplary embodiment, the first unit 20D1 and the second unit 20D2 are symmetrically disposed with respect to a reference plane including the position of an observation object S. In the ninth exemplary embodiment, as in the seventh exemplary embodiment, the optical components are disposed along an optical axis of object light such that an optical axis of object light having the first wavelength and being s-polarized light is coaxial with an optical axis of object light having the second wavelength and being p-polarized light. Further, in the ninth exemplary embodiment, as in the fifth exemplary embodiment, the first unit 20D1 and the second unit 20D2 are disposed such that a focal position of a lens 282 differs from a focal length of a lens 281.
Except that the “light having the first wavelength and being s-polarized light” is used in place of the light having the first wavelength, and the “light having the second wavelength and being p-polarized light” is used in place of the light having the second wavelength, the operation of the hologram generating unit according to the ninth exemplary embodiment is substantially the same as the operation of the hologram generating unit according to the seventh exemplary embodiment. Therefore, only differences are described.
Object light that has the first wavelength and is s-polarized light, and that exits from a first dividing optical element 261 in a second direction is such that the wavelength filter 301 removes light having wavelengths other than the first wavelength, and the polarizing filter 30S removes light other than the s-polarized light. Then, the lens 321 gathers the light, and an image capturing surface of a first image capturing unit 141 is irradiated with the gathered light.
Reference light that has the first wavelength and is s-polarized light and that exits from the first dividing optical element 261 in the second direction is such that the wavelength filter 301 removes light having wavelengths other than the first wavelength, and the polarizing filter 30S removes light other than the s-polarized light. Then, the lens 321 collimates the light, and the image capturing surface of the first image capturing unit 141 is irradiated with the collimated light.
The object light that has the first wavelength and is s-polarized light, and that illuminates the image capturing surface of the first image capturing unit 141 and the reference light that has the first wavelength and that is s-polarized light, and that illuminates the image capturing surface of the first image capturing unit 141 interfere with each other to generate an interference fringe at the image capturing surface of the first image capturing unit 141. The first image capturing unit 141 captures, as a first hologram, the interference fringe generated at the image capturing surface.
Object light that has the second wavelength and is p-polarized light, and that exits from a second dividing optical element 262 in a first direction is such that the wavelength filter 302 removes light having wavelengths other than the second wavelength, and the polarizing filter 30P removes light other than the p-polarized light. Then, the lens 322 gathers the light, and an image capturing surface of a second image capturing unit 142 is irradiated with the gathered light.
Reference light that has the second wavelength and is p-polarized light and that exits from the second dividing optical element 262 in the first direction is such that the wavelength filter 302 removes light having wavelengths other than the second wavelength, and the polarizing filter 30S removes light other than the p-polarized light. Then, the lens 322 collimates the light, and the image capturing surface of the second image capturing unit 142 is irradiated with the collimated light.
The object light that has the second wavelength and is p-polarized light, and that illuminates the image capturing surface of the second image capturing unit 142, and the reference light that has the second wavelength and that is p-polarized light, and that illuminates the image capturing surface of the second image capturing unit 142 interfere with each other to generate an interference fringe at the image capturing surface of the second image capturing unit 142. The second image capturing unit 142 captures, as a second hologram, the interference fringe generated at the image capturing surface.
In the ninth exemplary embodiment, the optical axis of the object light having the first wavelength and being s-polarized light and the optical axis of the object light having the second wavelength and being p-polarized light are coaxial. By disposing the optical components symmetrically with respect to the reference plane including the position of the observation object, the operation of the hologram generating unit 12 as a first hologram generating unit and the operation of the hologram generating unit 12 as a second hologram generating unit are performed at the same time. This enlarges a region including observation regions that are observed at the same time compared to the case in which the observation object is irradiated with light having a single wavelength and is observed.
As in the eighth exemplary embodiment, it is possible to irradiate the observation object with two types of light beams having different wavelengths and polarization directions to generate two types of object light beams and separate the generated two types of light beams by, for example, the wavelength filters and the polarizing filters. Therefore, by allowing the light to be transmitted through the polarizing filters in addition to through the wavelength filters, noise that is not capable of being completely removed by the wavelength filters is removed.
The structures of the digital holographic apparatuses according to the above-described exemplary embodiments are merely exemplifications. Accordingly, it goes without saying that these structures may be modified within a scope that does not depart from the gist of the present invention.
The digital holographic apparatus according to each of the above-described exemplary embodiments is also applicable to a digital holographic microscope. Even in the digital holographic microscope, as in an ordinary microscope, a region that is observed is reduced in size as the NA of objective lenses increases. When information about depth direction is important such as in measuring fluids, the region that is observed is limited in size.
For example, in measuring fluids, in order to observe the direction of flow, very small tracer particles are mixed with a fluid. As the diameters of the particles are reduced, an objective lens having a high NA is used for observing the positions of the particles. In this case, since an objective lens having a high NA is used, an area that is observed in a depth direction is reduced in size. As a result, it may not be possible to observe a desired region. In order to enlarge the region that is observed, the numbers of pixels of the image capturing units may be increased. However, this causes the image processing time to be increased.
According to the structures of the exemplary embodiments, it is possible to enlarge the region that is observed in a digital holographic microscope without increasing the number of pixels of the image capturing units.
Hitherto, reflection light generated by irradiating an observation object with light from one direction is recorded to measure the shape of the observation objection. However, in this method, in order to measure the surrounding of the observation object, the front and back sides of the observation object need to be reversed, which is troublesome.
According to the digital holographic apparatuses according to the exemplary embodiments, it is possible to observe an observation object from different observation directions, and to obtain at the same time a three-dimensional shape when a subject in the region that is observed is observed from one direction and a three-dimensional shape when the subject in the region that is observed is observed from a different direction.
Therefore, in the medical or biotechnological fields, it becomes easy to know the structure of an observation object whose structure is difficult to know because, for example, cells or microorganisms are very small. Even in other industrial fields, it becomes easy to know and examine the shape of very small structural bodies, such as toner particles, micro-electro mechanical systems (MEMS), or those in biomimetics.
The foregoing description of the exemplary embodiments of the present invention has been provided for the purposes of illustration and description. It is not intended to be exhaustive or to limit the invention to the precise forms disclosed. Obviously, many modifications and variations will be apparent to practitioners skilled in the art. The embodiments were chosen and described in order to best explain the principles of the invention and its practical applications, thereby enabling others skilled in the art to understand the invention for various embodiments and with the various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the following claims and their equivalents.
Number | Date | Country | Kind |
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2016-064332 | Mar 2016 | JP | national |
2016-064462 | Mar 2016 | JP | national |