Patent Application
20170315090
This present disclosure relates to digital microfluidic devices, and to electrochemical detection on via digital microfluidic devices.
Digital microfluidics is an emerging technology in which discrete liquid droplets are manipulated on the surface of an array of electrodes. Digital microfluidics has numerous complementary differences relative to conventional enclosed-microchannel-based fluidics, including reconfigurability (a generic device format can be used for any application) and control over all reagents. Digital microfluidics is typically implemented in a “two-plate” format, in which droplets are sandwiched between a bottom plate (bearing an array of electrodes coated with an insulator), and a top plate (bearing a ground electrode not coated with an insulator).
Devices and systems are provided in which one or more electrochemical sensors are integrated within a digital microfluidic device. According to one example embodiment, a two-electrode electrochemical sensor is integrated into a top or bottom plate of a digital microfluidic device, where the counter electrode is provided within a defined spatial region, and where the working electrode is formed such that it is spatially distributed within the spatial region associated with the counter electrode. The working electrode may be provided as one or more elongate segments that are spatially distributed within, and/or surround a perimeter of, the counter electrode. The area of the working electrode may be selected to be smaller than that of the counter electrode in order to improve the performance of the electrochemical sensor.
Accordingly, in a first aspect, there is provided a digital microfluidic device comprising:
a first plate comprising:
a second plate comprising:
wherein one of said first insulating substrate and said second insulating substrate has one or more digital microfluidic secondary electrodes provided thereon, and wherein said one or more digital microfluidic secondary electrodes are provided such that liquid droplets are transportable under application of voltages between said array of digital microfluidic actuation electrodes and said one or more digital microfluidic secondary electrodes; and
wherein one of said first insulating substrate and said second insulating substrate comprises an electrochemical working electrode and an electrochemical counter/pseudo-reference electrode for forming an electrochemical cell;
wherein said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode are exposed to the gap between said first plate and said second plate such that a droplet positioned between said first plate and said second plate at a location corresponding to said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode is in electrical communication with said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode; and
In another aspect, there is provided a digital microfluidic device comprising:
In another aspect, there is provided a digital microfluidic device comprising:
a first plate comprising:
a second plate comprising:
wherein one of said first insulating substrate and said second insulating substrate has one or more digital microfluidic secondary electrodes provided thereon, and wherein said one or more digital microfluidic secondary electrodes are provided such that liquid droplets are transportable under application of voltages between said array of digital microfluidic actuation electrodes and said one or more digital microfluidic secondary electrodes; and
wherein one of said first insulating substrate and said second insulating substrate comprises an electrochemical working electrode and an electrochemical counter/pseudo-reference electrode for forming an electrochemical cell, said electrochemical working electrode comprising a plurality of elongate segments;
wherein said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode are exposed to the gap between said first plate and said second plate such that a droplet positioned between said first plate and said second plate at a location corresponding to said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode is in electrical communication with said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode.
In another aspect, there is provided a digital microfluidic device comprising:
an insulating substrate;
an array of digital microfluidic actuation electrodes provided on said insulating substrate;
one or more digital microfluidic secondary electrodes provided on said insulating substrate;
a dielectric layer formed over said array of digital microfluidic actuation electrodes and said one or more digital microfluidic secondary electrodes; and
a hydrophobic layer provided on said dielectric layer, said hydrophobic layer providing a hydrophobic working surface, wherein said one or more digital microfluidic secondary electrodes are provided such that liquid droplets are transportable on said hydrophobic layer under application of voltages between said array of digital microfluidic actuation electrodes and said one or more digital microfluidic secondary electrodes;
wherein said insulating substrate further comprises an electrochemical working electrode and an electrochemical counter/pseudo-reference electrode for forming an electrochemical cell, said electrochemical working electrode comprising a plurality of elongate segments;
wherein said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode are exposed such that a droplet positioned on said hydrophobic working surface at a location corresponding to said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode is in electrical communication with said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode.
In another aspect, there is provided a digital microfluidic device comprising:
a first plate comprising:
a second plate comprising:
wherein one of said first insulating substrate and said second insulating substrate has one or more digital microfluidic secondary electrodes provided thereon, and wherein said one or more digital microfluidic secondary electrodes are provided such that liquid droplets are transportable under application of voltages between said array of digital microfluidic actuation electrodes and said one or more digital microfluidic secondary electrodes; and
wherein one of said first insulating substrate and said second insulating substrate comprises an electrochemical working electrode and an electrochemical counter/pseudo-reference electrode for forming an electrochemical cell, said electrochemical working electrode consists of one or more elongate segments;
wherein said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode are exposed to the gap between said first plate and said second plate such that a droplet positioned between said first plate and said second plate at a location corresponding to said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode is in electrical communication with said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode.
In another aspect, there is provided a digital microfluidic device comprising:
an insulating substrate;
an array of digital microfluidic actuation electrodes provided on said insulating substrate;
one or more digital microfluidic secondary electrodes provided on said insulating substrate;
a dielectric layer formed over said array of digital microfluidic actuation electrodes and said one or more digital microfluidic secondary electrodes; and
a hydrophobic layer provided on said dielectric layer, said hydrophobic layer providing a hydrophobic working surface, wherein said one or more digital microfluidic secondary electrodes are provided such that liquid droplets are transportable on said hydrophobic layer under application of voltages between said array of digital microfluidic actuation electrodes and said one or more digital microfluidic secondary electrodes;
wherein said insulating substrate further comprises an electrochemical working electrode and an electrochemical counter/pseudo-reference electrode for forming an electrochemical cell, said electrochemical working electrode consists of one or more elongate segments;
wherein said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode are exposed such that a droplet positioned on said hydrophobic working surface at a location corresponding to said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode is in electrical communication with said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode.
In another aspect, there is provided a digital microfluidic device comprising:
a first plate comprising:
a second plate comprising:
wherein one of said first insulating substrate and said second insulating substrate has one or more digital microfluidic secondary electrodes provided thereon, and wherein said one or more digital microfluidic secondary electrodes are provided such that liquid droplets are transportable under application of voltages between said array of digital microfluidic actuation electrodes and said one or more digital microfluidic secondary electrodes; and
wherein one of said first insulating substrate and said second insulating substrate comprises an electrochemical working electrode and an electrochemical counter/pseudo-reference electrode for forming an electrochemical cell;
wherein said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode are exposed to the gap between said first plate and said second plate such that a droplet positioned between said first plate and said second plate at a location corresponding to said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode is in electrical communication with said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode; and
wherein at least a portion of said electrochemical working electrode forms an elongate segment that surrounds at least a portion of a perimeter of said electrochemical counter/pseudo-reference electrode.
In another aspect, there is provided a digital microfluidic device comprising:
an insulating substrate;
an array of digital microfluidic actuation electrodes provided on said insulating substrate;
one or more digital microfluidic secondary electrodes provided on said insulating substrate;
a dielectric layer formed over said array of digital microfluidic actuation electrodes and said one or more digital microfluidic secondary electrodes; and
a hydrophobic layer provided on said dielectric layer, said hydrophobic layer providing a hydrophobic working surface, wherein said one or more digital microfluidic secondary electrodes are provided such that liquid droplets are transportable on said hydrophobic layer under application of voltages between said array of digital microfluidic actuation electrodes and said one or more digital microfluidic secondary electrodes;
wherein said insulating substrate further comprises an electrochemical working electrode and an electrochemical counter/pseudo-reference electrode for forming an electrochemical cell;
wherein said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode are exposed such that a droplet positioned on said hydrophobic working surface at a location corresponding to said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode is in electrical communication with said electrochemical working electrode and said electrochemical counter/pseudo-reference electrode,
wherein at least a portion of said electrochemical working electrode forms an elongate segment that surrounds at least a portion of a perimeter of said electrochemical counter/pseudo-reference electrode.
A further understanding of the functional and advantageous aspects of the disclosure can be realized by reference to the following detailed description and drawings.
Embodiments will now be described, by way of example only, with reference to the drawings, in which:
Various embodiments and aspects of the disclosure will be described with reference to details discussed below. The following description and drawings are illustrative of the disclosure and are not to be construed as limiting the disclosure. Numerous specific details are described to provide a thorough understanding of various embodiments of the present disclosure. However, in certain instances, well-known or conventional details are not described in order to provide a concise discussion of embodiments of the present disclosure.
As used herein, the terms “comprises” and “comprising” are to be construed as being inclusive and open ended, and not exclusive. Specifically, when used in the specification and claims, the terms “comprises” and “comprising” and variations thereof mean the specified features, steps or components are included. These terms are not to be interpreted to exclude the presence of other features, steps or components.
As used herein, the term “exemplary” means “serving as an example, instance, or illustration,” and should not be construed as preferred or advantageous over other configurations disclosed herein.
As used herein, the terms “about” and “approximately” are meant to cover variations that may exist in the upper and lower limits of the ranges of values, such as variations in properties, parameters, and dimensions. Unless otherwise specified, the terms “about” and “approximately” mean plus or minus 25 percent or less.
It is to be understood that unless otherwise specified, any specified range or group is as a shorthand way of referring to each and every member of a range or group individually, as well as each and every possible sub-range or sub-group encompassed therein and similarly with respect to any sub-ranges or sub-groups therein. Unless otherwise specified, the present disclosure relates to and explicitly incorporates each and every specific member and combination of sub-ranges or sub-groups.
As used herein, the term “on the order of”, when used in conjunction with a quantity or parameter, refers to a range spanning approximately one tenth to ten times the stated quantity or parameter.
Unless defined otherwise, all technical and scientific terms used herein are intended to have the same meaning as commonly understood to one of ordinary skill in the art.
As used herein, the term “two-electrode electrochemical sensor” refers to an electrochemical sensor including a working electrode and a second electrode, where the second electrode is referred to herein as a “counter electrode” or a “pseudo reference” electrode.
Digital microfluidics (DMF) is an emerging technology in which discrete liquid droplets are manipulated on the surface of an array of electrodes. The technology has numerous complementary advantages relative to conventional enclosed-microchannel-based fluidics, including reconfigurability (a generic device format can be used for any application) and capability to integrate a wide range of sample processing operations. Digital microfluidics is typically implemented in a “two-plate” format, in which droplets are sandwiched between a bottom plate (bearing an array of electrodes coated with an insulator), and a top plate (bearing a counter-electrode not coated with an insulator). Droplets are made to move by applying potentials between bottom plate and top-plate electrodes. The top plate electrodes are often formed from transparent indium tin oxide (ITO) to enable visualization of droplet movement. A conventional two-plate digital microfluidic device is illustrated in
Spaced above actuation electrodes 14 and dielectric layer 16, on the other side of gap 30, is a second plate, where the spacing may be achieved using a spacer (not shown). Continuous secondary electrode 22 (or a plurality of secondary electrodes) is provided on an insulating substrate 24 (secondary electrode 22 is often referred to in the digital microfluidic literature as a reference electrode, but the term “secondary” is employed herein in order to avoid confusion with the reference electrode of an electrochemical sensor), and a hydrophobic layer 20 (for example Teflon AFTM, 50 nm) is coated on secondary electrode 22. Alternatively, another dielectric layer can be deposited between layers 20, 22.
Liquid droplets 42 are provided within gap 30 between two hydrophobic layers 18 and 20. Electrodes 14, voltage source 26, and the continuous secondary electrode 22 together form an electric field, digitally manipulated by controller 28. For droplet manipulation, secondary electrode 22 is biased to a potential different from the actuating potential. A commonly used reference potential for secondary electrode 22 is ground. The upper hydrophobic layer 20, secondary electrode 22, and substrate layer 24 may be substantially transparent to allow optical detection, such as optical imaging or optical analysis of digital microfluidic assays.
While some aspects of the present disclosure involve adaptations of the two-plate design of
Referring now to
In the example embodiment shown in
As described below, the sensor electrodes may be formed from the same planar electrode material that defines the secondary electrode of the digital microfluidic device. For example, the sensor electrodes may be etched on the top plate out of a continuous plate of ITO (using photolithography and wet etching), and separated from the much larger secondary electrode used to enable DMF droplet manipulation. In such an embodiment, the entire top plate may be coated with Teflon-AF, except for the sensor-electrodes, which are exposed, for example, by a lift-off process.
Various example embodiments provided herein overcome drawbacks associated with known implementations of electrochemical sensors in digital microfluidic devices. Prior implementations typically either (a) require complex multistep fabrication processes and are thus likely expensive to manufacture, or (b) simply use external electrodes with no integration, and are thus not suitable for mass production. Moreover, as demonstrated in the examples below, ratio of electrode areas according to various embodiments disclosed herein allow for low detection limits that are likely unmatched by any of the previously reported methods, except for methods relying on fragile nanostructured metal electrodes.
Referring to
In contrast, various example embodiments of the present disclosure provide electrode configurations in which the working electrode is spatially distributed within a spatial region associated with the counter electrode. For example,
Another set of example electrode configurations are shown in
Referring now to
Examples of electrode configurations that have been fabricated and experimentally tested are shown in
Although the preceding examples show a counter electrode having a circular profile, it will be understood that the counter electrode can take on a wide variety of other shapes, such as square, elliptical, polygonal, and the like.
It is also noted that although the examples provided herein involve electrochemical sensors based on a two-electrode configuration, the embodiments provided herein may be adapted or modified to include one or more additional electrodes, such as an electrochemical reference electrode.
In a two-electrode electrochemical cell, the counter electrode also acts as the reference electrode, simplifying manufacturing and setup process. However, the lack of the third reference electrode removes the ability for it to maintain the accurate potential difference between the counter and working electrode. Therefore, a two-electrode cell with a conventional ratio of the area between the counter electrode and the reference electrode, namely ACE/AWE<2 (e.g. as shown in
However, by increasing ACE/AWE, according to various embodiments of the present disclosure, one can obtain a larger surface area that can absorb larger amounts current. In some embodiments, the area ratio of the areas of the counter electrode and reference electrode are provide such that 5<ACE/AWE<15. Within this ratio range, there is sufficient surface area of the counter electrode that normal currents generated by the electrochemical cell are small enough such that they do not charge the counter electrode to a sufficient degree to bias the recording signal.
It is noted that an area ratio beyond this range may result in performance degradation for the following reasons: (i) at larger ACE/AWE ratios, too much of the droplet is not exposed to the working electrode, reducing efficiency of the electrochemical cell; and (ii) larger ACE/AWE ratios necessarily require more space on the system, reducing ability to form multiplexed arrays of electrodes and reducing throughput. Notwithstanding these potential problems, embodiments may be realized in which the area ratio exceeds 15, such as an area ratio that exceeds 20, 50, or 100.
In some embodiments, the working electrode is provided as one or more elongate segments, as noted above. The width of the elongate segments may be selected, and/or optimized, based on analyte of interest and the desired concentration range. In the some example implementations, the width of at least a portion of one or more segments of the working electrode may be between approximately 1 and 10 microns, between approximately 10 and 100 microns, or between approximately 100 and 500 microns. A width in the range of 50-200 microns may be suitable for a wide range of analytes.
It is noted that at a width of approximately 1 micron, the working electrode is the most sensitive, as normal linear diffusion is replaced by radial diffusion, allowing the electrode to come into contact with a higher amount of analyte at a lower concentration. On the other hand, at 500 microns, the electrode is most suitable for analytes with low diffusivity or large sizes, these analytes need more time and space to diffuse onto the electrodes.
Although many of the examples provided herein employ ITO for forming the electrochemical electrodes, it will be understood that ITO is but one example of a suitable conductive material for forming one or both of the electrochemical electrodes. Examples of other conductive materials that may be employed for forming one or both of the electrochemical electrodes include, but are not limited to, gold, silver, carbon and platinum. For example, electrochemical electrodes may be formed as gold vs. gold, silver vs. silver, carbon vs. carbon, platinum vs. platinum, and combinations thereamong, such as gold vs. silver, gold vs. platinum, gold vs. carbon, and the like, as will be apparent to one skilled in the art of electrochemical detection.
In one example implementation, such electrodes may be formed on an underlying ITO layer. Example methods of fabricating such electrodes on ITO (with the exception of carbon) are provided in Shamsi, M. H.; Choi, K.; Ng, A. H. C.; Wheeler, A. R. “A digital microfluidic electrochemical immunoassay” Lab on a Chip 2014, 14, 547-554.
Example System for Performing Electrochemical Measurements with a Digital Microfluidic Device
Referring now to
Although only one of each component is illustrated in
In one embodiment, control and processing unit 225 may be, or include, a general purpose computer or any other hardware equivalents. Control and processing unit 225 may also be implemented as one or more physical devices that are coupled to processor 230 through one of more communications channels or interfaces. For example, control and processing unit 225 can be implemented using application specific integrated circuits (ASICs). Alternatively, control and processing unit 225 can be implemented as a combination of hardware and software, where the software is loaded into the processor from the memory or over a network connection.
Control and processing unit 225 may be programmed with a set of instructions which when executed in the processor causes the system to perform one or more methods described in the disclosure. Control and processing unit 225 may include many more or less components than those shown.
While some embodiments have been described in the context of fully functioning computers and computer systems, those skilled in the art will appreciate that various embodiments are capable of being distributed as a program product in a variety of forms and are capable of being applied regardless of the particular type of machine or computer readable media used to actually effect the distribution.
A computer readable medium can be used to store software and data which when executed by a data processing system causes the system to perform various methods. The executable software and data can be stored in various places including for example ROM, volatile RAM, non-volatile memory and/or cache. Portions of this software and/or data can be stored in any one of these storage devices. In general, a machine readable medium includes any mechanism that provides (i.e., stores and/or transmits) information in a form accessible by a machine (e.g., a computer, network device, personal digital assistant, manufacturing tool, any device with a set of one or more processors, etc.).
Examples of computer-readable media include but are not limited to recordable and non-recordable type media such as volatile and non-volatile memory devices, read only memory (ROM), random access memory (RAM), flash memory devices, floppy and other removable disks, magnetic disk storage media, optical storage media (e.g., compact discs (CDs), digital versatile disks (DVDs), etc.), among others. The instructions can be embodied in digital and analog communication links for electrical, optical, acoustical or other forms of propagated signals, such as carrier waves, infrared signals, digital signals, and the like.
Some aspects of the present disclosure can be embodied, at least in part, in software. That is, the techniques can be carried out in a computer system or other data processing system in response to its processor, such as a microprocessor, executing sequences of instructions contained in a memory, such as ROM, volatile RAM, non-volatile memory, cache, magnetic and optical disks, or a remote storage device. Further, the instructions can be downloaded into a computing device over a data network in a form of compiled and linked version. Alternatively, the logic to perform the processes as discussed above could be implemented in additional computer and/or machine readable media, such as discrete hardware components as large-scale integrated circuits (LSI's), application-specific integrated circuits (ASIC's), or firmware such as electrically erasable programmable read-only memory (EEPROM's) and field-programmable gate arrays (FPGAs).
Embodiments of the present disclosure may be employed for a variety of assays that employ electrochemical detection (particularly those that require sensitive analysis of trace amounts of analyte). One example application, which is illustrated in the examples provided below, involves the sensitive measurement of dopamine concentration using a two-electrode electrochemical sensor that is configured according to the example embodiments disclosed herein. It will be understood that the example application involving dopamine measurement is provided merely as an illustrative example application, and that many other applications may be realized by selecting different analytes (and optionally different electrode compositions).
Although the preceding example embodiments, and the examples provided herebelow, relate to two-plate designs with one or more electrochemical sensors integrated into the top plate of a two-plate device, it will be understood that various other alternative embodiments may be realized by additionally, or alternatively, incorporating one or more electrochemical sensors into the bottom plate of a two-plate device, or into a single-plate device. For example, the bottom plate of a two-plate device, or a single plate of an open digital microfluidic device, may be adapted to include one or more electrochemical sensors incorporating a working electrode and a counter electrode based on the embodiments described herein.
In a single-plate device, the electrochemical electrodes may be formed on the bottom plate, in addition to both the actuation electrodes and the secondary digital microfluidic electrodes. For example, electrochemical electrodes may be provided within a region of a single plate that would otherwise have been occupied by a portion of an actuation electrode.
In another example implementation, in a two-plate device, electrochemical electrodes can be formed on the bottom plate, coplanar to the actuation electrodes, or in a different plane on the bottom plate, provided that the electrochemical electrodes are in electrical communication with droplets translated on the working surface of the device (e.g. exposed electrodes). For example,
The following examples are presented to enable those skilled in the art to understand and to practice embodiments of the present disclosure. They should not be considered as a limitation on the scope of the disclosure, but merely as being illustrative and representative thereof.
A digital microfluidic device with an integrated electrochemical sensor was formed as described below. The electrodes were characterized by cyclic voltammetry of 2 mM ferro/ferricyanide.
An on-chip serial dilution was then conducted to determine the sensitivity of the system to dopamine, with a limit of detection of 20.3 nM, well below the level expected from physiological dopamine spikes. Results from on-chip analysis of dopamine, using two-electrode electrochemical cells formed as described below, are shown in
The patterns were generated by using transparent photomasks printed at 20,000 DPI. The bottom-plates of DMF devices bearing an array of electrodes were formed by standard photolithography and wet etching. Briefly, chromium- (200 nm thick) and photoresist-coated glass substrates (2″×3″×1.1 mm) were exposed to UV light through a photomask using a Suss MicroTec mask aligner (29.8 mW/cm2, 10 seconds). The exposed substrates were developed in MF-321 (3 min) and post-baked on a hot plate (125° C., 1 min). The developed substrates were etched in CR-4 (3 min) and the remaining photoresist was stripped in AZ300T (5 min).
After forming electrodes, the substrates were primed for parylene coating by immersing in silane solution (2-propanol, DI water, A-174, and acetic acid 50:50:1:2 v/v/v/v, 10 min) and curing on a hot-plate (80° C., 10 min). After rinsing and drying, devices were coated with ˜7 μm of Parylene C (vapor deposition) and ˜200 nm of Teflon-AF (spin-coating, 1% w/w in Fluorinert FC-40, 2000 rpm, 60 s), and post-baked on a hot-plate (165° C., 10 min). The polymer coatings were removed from contact pads by gentle scraping with a scalpel.
Top-plates of DMF devices were formed from indium-tin oxide (ITO) coated glass substrates in three stages.
In the first stage, the substrates were sonicated in acetone for 5 min and rinsed in 2-propanol for 1 min. After drying and dehydrating, substrates were spin-coated (3000 RPM, 45 s) with Shipley S1811 photoresist (Marlborough, Mass.) and then post-baked on a hot plate (95° C., 2 min). Subsequently, the substrates were exposed (29.8 mW cm−2, 10 s) through a mask. The substrates were developed for 3 min by immersing in MF-321 (MicroChem, Newton, Mass.), post-baked on a hot plate (125° C., 1 min), and then etched for 10 min by immersing in ITO etchant comprising 4:2:1 (v/v/v) hydrochloric acid, deionized (DI) water, and nitric acid. After rinsing, the remaining photoresist was stripped for 5 min by immersing in AZ300T (Capitol Scientific Inc., Texas). When complete, the ITO on the device was separated into seven isolated regions, including six electroanalysis electrodes (four 1.6 mm diameter circles and two 1.2×1.2 mm squares) and one large, irregularly shaped DMF driving electrode. Each electroanalysis electrode was connected to a contact pad on the edge of the substrate.
In the second stage, a spin-coat/lift-off process was performed, as described here in detail. ITO-glass slides were immersed in RCA solution (6:1:1 DI water: 28% aqueous ammonium hydroxide: 30% hydrogen peroxide) for 15 min at 80° C. After rinsing, drying, and dehydrating, substrates were spin-coated with Shipley S1811 photoresist (3000 RPM, 60 s) and then post-baked on a hot plate (2 min, 95° C.). The substrates were exposed (10 s, 29.8 mW cm−2) through a mask bearing features of the two electrochemical electrodes and then developed in MF-321.
After rinsing and drying, the substrates were flood exposed (10 s, 29.8 mW cm−2), and then spin-coated with Teflon-AF and post-baked using the same parameters used for bottom-plate substrates (as above). The substrates were then immersed in acetone with gentle agitation until the Teflon-AF over the patterned sites was lifted off (5-10 s). After rinsing and drying, the Teflon-AF was reflowed by baking on a hot plate at 165° C. and 230° C. for 5 min at each temperature.
The specific embodiments described above have been shown by way of example, and it should be understood that these embodiments may be susceptible to various modifications and alternative forms. It should be further understood that the claims are not intended to be limited to the particular forms disclosed, but rather to cover all modifications, equivalents, and alternatives falling within the spirit and scope of this disclosure.
This application claims priority to U.S. Provisional Application No. 62/066,818, titled “DIGITAL MICROFLUIDIC DEVICES WITH INTEGRATED ELECTROCHEMICAL SENSORS” and filed on Oct. 21, 2014, the entire contents of which is incorporated herein by reference.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CA2015/051066 | 10/21/2015 | WO | 00 |
| Number | Date | Country | |
|---|---|---|---|
| 62066818 | Oct 2014 | US |
