Claims
- 1. A labeled probe, comprising:
- a first moiety selected from the group consisting of a nucleic acid, peptide nucleic acid, protein, steroid, carbohydrate, pharmaceutical drug, non-pharmaceutical drug, and a non-drug hapten, wherein said first moiety corresponds to a target component of a sample, said first moiety bearing bound to it a 1,2-dioxetane moiety which, upon exposure to a suitable trigger, decomposes to release light.
- 2. The labeled probe of claim 1, wherein said probe is of the formula ##STR13## wherein either X or Y is a linking group bonded to said first moiety, and the remaining group X or Y is a carbon-containing moiety of 1-20 carbon atoms, selected from the group consisting of alkyl, heteroalkyl, heteroaryl, heteroaralkyl, aryloxy, hydrogen, aryl, substituted aryl, hydroxyaryl, acyloxyaryl, silyloxyaryl, aminoaryl and sulfonamidoaryl.
- 3. The labeled probe of claim 2, wherein said linking group comprises an alkyl group of 1-11 carbon atoms.
- 4. A method of detecting the presence of a compound in a sample, comprising, hybridizing any said compound in a sample with a labeled probe of claim 1,
- exposing said hybridized compound to conditions which induce the dioxetane moiety on said labeled probe with which said compound has been hybridized to decompose, and
- detecting any emission of light in said sample, wherein said light emission is indicative of said compound being present in said sample.
- 5. The method of claim 4, wherein the amount of light is quantified, and is directly related to the amount of said compound present in said sample.
Parent Case Info
This application is a Continuation of application Ser. No. 08/767,479, filed on Dec. 16, 1996 now U.S. Pat. No. 5,800,999.
Foreign Referenced Citations (1)
Number |
Date |
Country |
0401001 |
Dec 1990 |
EPX |
Continuations (1)
|
Number |
Date |
Country |
Parent |
767479 |
Dec 1996 |
|