Claims
- 1. A process for the preparation of a dispersion polymer, which comprises the steps of first preparing a latex of a non-film-forming seed polymer having an incompletely polymerized monomer content of 5 to 50% by weight by carrying out the polymerization so that only 30% to 90% of the monomer present is polymerized completely, subsequently separating off the monomers not bonded to the polymer and then polymerizing a monomer mixture which contains at least one compound of the formula I containing acetal groups ##STR5## wherein n is 1-6; R.sub.1 is H or CH.sub.3 ; and
- R.sub.2 and R.sub.3 are identical or different, where
- R.sub.2 and R.sub.3 are (CH.sub.2).sub.m --CH.sub.3, and m is 0-7, or ##STR6## in which X, Y and Z are (CH.sub.2).sub.p --CH.sub.3 and P is 1--3, where X, Y and Z are identical or different, or R.sub.2 and R.sub.3 are an aryl radical,
- and, in addition, up to 30% by weight, based on the mixture, of monomrs selected from acrylic acid, methacrylic acid, crotonic acid and mixtures thereof, and a further up to 30% by weight, again based on the total mixture, of carbocyclic aromatic vinylidene monomers in the presence of the seed polymer thus obtained.
- 2. A process for the preparation of a dispersion polymer as recited in claim 1, wherein said non-film-forming seed polymer is polystyrene and said carbocyclic aromatic vinylidene monomer is styrene.
- 3. A process for the preparation of a dispersion polymer as recited in claim 1, wherein said non-film-forming seed polymer is comprised of polymerized carbocyclic aromatic monovinylidene monomers.
- 4. A process for the preparation of a dispersion polymer as recited in claim 3, wherein said carbocyclic aromatic monovinylidene monomers are selected from the group consisting of styrene, vinyltoluene, vinylnaphthalene and mixture thereof.
- 5. A process for the preparation of a dispersion polymer as recited in claim 4, wherein said non-film-forming seed polymer has a particle diameter in the range of from about 0.05 to 0.5 .mu.m.
- 6. A process for the preparation of a dispersion polymer as recited in claim 4, wherein said non-film-forming seed polymer, has an incompletely polymerized monomer content of about 20 to 30% by weight.
- 7. A process for the preparation of a dispersion polymer as recited in claim 6, wherein said non-film-forming seed polymer is formed by carrying out the polymerization so that only about 50 to 70% of the monomer present is polymerized completely.
- 8. A process for the preparation of a dispersion polymer as recited in claim 4, wherein said compound of the formula I is selected from the group consisting of an acryl- or methacrylamidoalkylaldehyde di-alkyl acetal having alkyl groups of from 2 to 8 carbon atoms.
- 9. A process for the preparation of a dispersion polymer as recited in claim 7, wherein said compound of the formula I is selected from the group consisting of an acryl- or methacrylamidoacetaldehyde di-n-pentyl acetal.
- 10. A process for the preparation of a dispersion polymer as recited in claim 9, wherein said monomer mixture containing at least one compound of the formula I is added dropwise to the latex of non-film-forming seed polymer which is heated to a temperature of from about 50.degree. C. to 120.degree. C.
- 11. A process for the diagnostic detection of antigens, antibodies or haptens in a sample which comprises:
- (a) incubating said sample with a biologically active dispersion polymer comprising biologically active agents, selected from antigens, antibodies or haptens which will bind with the antigen, antibody or hapten to be detected, bonded to a dispersion polymer which contains latex particles having an incompletely polymerized monomer content of 5 to 50% by weight, based on the latex particles, having on the surface of said latex particles a copolymer of a monomer mixture which contains at least one of the compounds of the formula I containing acetal groups ##STR7## wherein n is 1-6;
- R.sub.1 is H or CH.sub.3 ; and
- R.sub.2 and R.sub.3 are identical or different, where
- R.sub.2 and R.sub.3 are (CH.sub.2).sub.m --CH.sub.3, and m is 0-7, or ##STR8## in which X, Y and Z are (CH.sub.2).sub.p --CH.sub.3 and P is 1-3, where X, Y and Z are identical or different, or R.sub.2 and R.sub.3 are an aryl radical; and
- (b) subjecting said biologically active dispersion polymer incubated with said sample to a means for detecting said antigen, antibody or hapten to be detected.
- 12. A process for the diagnostic detection of antigens, antibodies or haptens as recited in claim 11 wherein said means for detecting said antigen, antibody or hapten to be detected is selected from a nephelometric, turbidometric or particle counting method.
- 13. A process for the preparation of a biologically active dispersion polymer comprising the steps of:
- (1) preparing a latex of non-film-forming seed polymer having an incompletely polymerized monomer content of from about 5 to 50% by weight;
- (2) polymerizing in the presence of the seed polymer a monomer mixture which contains at least one compound of the formula I containing acetal groups ##STR9## wherein n is 1-6; R.sub.1 is H or CH.sub.3 ; and
- R.sub.2 and R.sub.3 are identical or different, where R.sub.2 and R.sub.3 are (CH.sub.2).sub.m --CH.sub.3, and m is 0-7, or ##STR10## in which X, Y and Z are (CH.sub.2).sub.p --CH.sub.3 and P is 1-3, where X, Y and Z are identical or different, or R.sub.2 and R.sub.3 are an aryl radical,
- and, in addition, up to 30% by weight, based on the mixture, of monomers selected from acrylic acid, methacrylic acid, crotonic acid and mixtures thereof, and a further up to 30% by weight, again based on the total mixture, of carbocyclic aromatic vinylidene monomers;
- (3) adjusting the pH of the resulting polymer latex to a pH of less than about 5; and
- (4) incubating the resultant polymer latex with the biologically active material to be bound thereto in the presence of a reducing agent.
- 14. A process for the preparation of a biologically active dispersion polymer as recited in claim 13, wherein said reducing agent comprises a solution of sodium cyanoborohydride in a neutral buffer.
- 15. A process for the preparation of a biologically active dispersion polymer as recited in claim 14, wherein said biologically active material is selected from the group consisting of antibodies, antigens and hapten.
- 16. A process for the preparation of a biologically active dispersion polymer as recited in claim 15, wherein said non-film-forming seed polymer is formed of polymerized monomers selected from the group consisting of styrene, vinyltoluene and vinylnaphthalene, and said compound of the formula I is selected from the group consisting of an acryl- or methacrylamidoacetaldehyde di-n-pentyl acetal.
Priority Claims (1)
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3613111 |
Apr 1986 |
DEX |
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Parent Case Info
This is a division of application Ser. No. 07/039,107, filed Apr. 16, 1987, now U.S. Pat. No. 4,962,046.
US Referenced Citations (9)
Divisions (1)
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Number |
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39107 |
Apr 1987 |
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