Patent Application
20240207414
The present application relates to a compound having the formula (I). The compound having the formula (I) is capable of releasing a molecular cargo in the presence of a reductant and is thus suitable for treating, ameliorating, preventing or diagnosing a disorder selected from a neoplastic disorder; atherosclerosis; an autoimmune disorder; an inflammatory disease; a chronic inflammatory autoimmune disease; ischaemia; and reperfusion injury.
In particular, the molecular cargo can be a therapeutic or diagnostic agent. In particular, the reductant can be a biological disulfide reductase or its effector disulfide protein which may be catalytically active. The invention further relates to a pharmaceutical or diagnostic composition comprising the same.
Dithiol/disulfide-exchange redox reactions are critical in a great number of biological pathways. Often, these are coordinated through conserved, specialised networks of oxidoreductases that perform redox reactions upon or using disulfides and/or thiols. The thioredoxin reductase-thioredoxin (TrxR-Trx) system, and the glutathione reductase-glutathione-glutaredoxin (GR-GSH-Grx) system, are two of the central redox systems; other key reductive enzymes/proteins include but are not limited to peroxiredoxins, glutathione peroxidases, glutathione-S-transferases, methionine sulfoxide reductase, and protein disulfide isomerases (PDIs) (Lee, S. et al. Antioxid Redox Signal 2013, 18, 1165-1207. https://doi.org/10.1089/ars.2011.4322). Such redox systems drive reactions vital to cellular metabolism, and can also regulate protein activity, protein-protein interactions, and protein localisation by reversible dithiol/disulfide-type reactions (Jones, D. P. et al. Antioxidants & Redox Signaling 2015, 23, 734-746. https://doi.org/10.1089/ars.2015.6247).
To better investigate and understand biological processes, it is of great interest to develop specific methods to image or quantify the activity of biological species, or otherwise to respond to their activity. Therefore it was an object of the present invention to provide compounds which have high selectivity for an oxidoreductase or its redox effector protein and which can be reduced by the disulfide reductive activity of that enzyme and thus release a molecular cargo. This has been a longstanding goal of research in the field of cellular dithiol/disulfide-exchange redox reactions. In this field, a significant challenge for chemical probe development is to ensure that a probe is specific for being reduced by the targeted species, and is not significantly reduced by any other reducing species in the cell. Therefore, to selectively probe one of the species TrxR, GR, Trx, or Grx, a compound must not be activated by any of the others or by GSH. This is difficult because these reductants can perform similar chemical reactions, and because the most active of these reductants have the lowest cellular concentrations (TrxR and GR have nM cellular concentrations; Trx and Grx have μM concentrations) while the least active reductant GSH is the most concentrated (mM).
These reducing species all act upon disulfides, so most reduction-activated chemical probes in the prior art have used disulfides as reduction-sensing motifs. Probes based on linear disulfides (such as E1) have been extensively reported, but it has been shown since decades that these disulfides are nonspecifically reduced by thiols including by GSH (Lim, C. S. et al. J. Am. Chem. Soc. 2011, 133, 11132-11135. https://doi.org/10.1021/ja205081s; Butora, G. et al. Angewandte Chemie International Edition 2014, 53, 14046-14050. https://doi.org/10.1002/anie.201407130). Therefore, linear disulfides are not suitable as selective probes in a cellular context.
Recently, there has been limited development of probes using cyclic disulfides as reduction-sensing motifs. For example, Fang and coworkers have developed several so-called “TRFS” probes and prodrugs (such as E2; Zhang, L. et al. J. Am. Chem. Soc. 2014, 136, 226-233. https://doi.org/10.1021/ja408792k) based on a cyclic five-membered disulfide motif; most of these release a cargo after reduction of the disulfide. However, it has been shown that this type of disulfide too is nonspecifically reduced by monothiols including by GSH, and therefore that five-membered cyclic disulfides are not suitable as the basis for oxidoreductase-selective probes in a cellular context (Felber, J. et al. ChemRxiv 2020. https://doi.org/10.26434/chemrxiv.13483155.v1).
As far as we are aware, only three concepts have been disclosed of probe or prodrug designs based on cyclic six-membered disulfides that are intended to release a general molecular cargo after reduction inside cells.
Firstly, in 2014, Butora disclosed a six-membered cyclic disulfide E3 capable of releasing a phosphate molecular cargo (R′O)(RO)P(O)OH after reduction. E3 was applied to cells, and the report stated that release was effected by intracellular GSH (Butora, G. et al. Angewandte Chemie International Edition 2014, 53, 14046-14050. https://doi.org/10.1002/anie.201407130; WO 2014/088923). The approach has since been used by e.g. Hayashi in 2017 (Hayashi, J. et al. Bioorganic & Medicinal Chemistry Letters 2017, 27, 3135-3138. https://doi.org/10.1016/j.bmcl.2017.05.031).
Secondly, in 2017, Kong et al. reported a six-membered cyclic disulfide E4 intended to release an aniline molecular cargo after reduction. E4 was applied to cells, and the report stated that release was effected by intracellular H2Se (Kong, F. et al. Anal. Chem. 2017, 89, 688-693. https://doi.org/10.1021/acs.analchem.6b03136). A similar concept was used by Li et al. in 2019 in a six-membered cyclic disulfide probe intended to release a different aniline cargo (molecule TRFS7 in their report) but the report stated that “the 1,2-dithianes cannot be reduced by either TrxR or GSH”; a related six-membered cyclic disulfide probe (molecule TRFS8 in their report) featuring a urea linkage was not intended to release a molecular cargo and indeed did not release it (Li, X. et al. Nature Communications 2019, 10, 2745. https://doi.org/10.1038/s41467-019-10807-8).
Thirdly, in 2017, Ziv disclosed prodrugs where a 4-amino-1,2-dithiane is linked to a phenolic molecular cargo as the carbamate, so as to release the phenol following reduction which was ascribed to occur through the “ambient reductive environment” of the cell although no cellular reductive species were demonstrated. An example of this is shown as molecule E5 (WO 2017/017669; U.S. Pat. No. 9,687,556). Somewhat relatedly, Kohn has worked on six-membered cyclic disulfide derivatives, although not intended to release a cargo. In 2004, Kohn published the molecule E6 (Lee, S. H. et al. J. Am. Chem. Soc. 2004, 126, 4281-4292. https://doi.org/10.1021/ja030577r). In this molecule three of the atoms in the piperazine-like ring that is annelated to the cyclic disulfide are sp2-configured, which rigidifies the system and prevents it from adopting a decalin-like geometry that would stabilise the disulfide. Notably, E6 was reported to be transthiolated by “an intracellular thiol or albumin” (i.e. by a monothiol) then to subsequently undergo a cyclisation that results in the attached mitomycin moiety becoming reactive. In 2005, Lee et al. published a related mitomycin derivative containing a six-membered cyclic disulfide that likewise did not release a molecular cargo after activation (Lee, S. H. et al. Org. Biomol. Chem. 2005, 3, 471-482. https://doi.org/10.1039/B414806A).
Therefore the relevant prior art in disulfides capable of releasing a molecular cargo after reduction contains (i) linear and cyclic five-membered disulfide designs that are unstable to monothiols such as GSH and therefore in a cellular context cannot be selective for a particular reductant; and (ii) monocyclic six-membered disulfide designs such as E3, E4, and E5, for which the literature is contradictory about what the relevant cellular reductant is (suggesting it is respectively GSH, H2Se, or an undefined ambient reductive environment, or alternatively stating that GSH cannot reduce the cyclic six-membered disulfide). The example of the geometrically strained six-membered disulfide E6, or the strained cyclic five-membered disulfides such as E2 (discussed in Felber, J. et al. ChemRxiv 2020. https://doi.org/10.26434/chemrxiv.13483155.v1) shows that destabilisation of the disulfide enhances its reactivity to reductants, which makes the disulfides sensitive to monothiols including GSH (and therefore in a cellular context they cannot be selective for a particular reductant).
Therefore there remain numerous unsolved challenges, including to: (i) design cyclic disulfide-based probe or prodrug systems that release a molecular cargo and that feature optimised disulfide ring geometries that stabilise the disulfide; and to (ii) design disulfide-based probe or prodrug systems that release a molecular cargo and that are selective for a particular cellular reductant, i.e. are shown to resist reduction by other major cellular reductants (e.g. GSH, TrxR, Trx, Grx, GR etc. as appropriate).
Under pathological conditions, homeostasis in these redox systems and in their target proteins is significantly dysregulated. This has been particularly shown in diseases such as cancer, inflammatory diseases such as autoimmune disorders, under acute stress such as reperfusion injury, and under other chronic conditions such as cardiovascular disease (Mohammadi, F. et al. Cancer Chemotherapy and Pharmacology 2019. https://doi.org/10.1007/s00280-019-03912-4, Whayne, T. F. et al. Can. J. Physiol. Pharmacol. 2015, 93, 903-911. https://doi.org/10.1139/cjpp-2015-0105). Such dysregulation has also been studied in the context of disease-associated biomarkers, such as hypoxia. Hypoxia is a pathology-associated feature, prevalent in cancer and inflammation, that is tightly correlated with significant biochemical changes including oxidoreductase dysregulation, many of which rely on the hypoxia-dependent transcription factor HIF-1. Oxidoreductase dysregulation may be reflected in a combination of changes to their expression level, enzymatic activity, redox poise (the ratio of oxidised to reduced form of the oxidoreductase), localisation, and/or other parameters. For example, under pathological conditions, the TrxR-Trx and GR-GSH-Grx systems act as repair systems, counteracting acute cellular damage by oxidants, and re-normalising redox imbalances e.g. from oxygen depletion (hypoxia) or from the switching of metabolic pathways due to cellular stress (e.g. the Warburg Effect in cancer; Arnér, E. S. J. et al. Seminars in Cancer Biology 2006, 16, 420-426. https://doi.org/10.1016/j.semcancer.2006.10.009, Karlenius, T. C. et al. Cancers 2010, 2, 209-232. https://doi.org/10.3390/cancers2020209); therefore cells affected by pathologies that require such repair and renormalisation are often found to upregulate expression levels and activity of these oxidoreductase systems, and shift their redox poise (Jiang, J. et al. Nanoscale 2014, 6, 12104-12110. https://doi.org/10.1039/C4NR01263A). This dysregulation has been particularly studied in the context of cancer, where for the example of Trx it has been shown that cycling hypoxia, which is a hallmark of most tumors, upregulates Trx expression levels (Karlenius, T. C. et al. Biochemical and Biophysical Research Communications 2012, 419, 350-355. https://doi.org/10.1016/j.bbrc.2012.02.027); that Trx overexpression contributes to hallmarks of cancer including increased proliferation and angiogenesis, and evasion of apoptosis (Powis, G. et al. Current Opinion in Pharmacology 2007, 7, 392-397. https://doi.org/10.1016/j.coph.2007.04.003); and that Trx overexpression in tumors contributes to chemoresistance and is associated to poor patient survival (Biaglow, J. E. et al. Cancer Biology & Therapy 2005, 4, 13-20. https://doi.org/10.4161/cbt.4.1.1434). Dysregulated function is also particularly studied in the context of inflammatory diseases, since many central players in inflammation (NFκB, TNF-α, Keap1, Nrf2) are regulated by these oxidoreductases, and processes from inflammasome activation to immune cell chemotaxis have also been shown to depend on these oxidoreductases (Bertini, R. et al. J Exp Med 1999, 189, 1783-1789. https://doi.org/10.1084/jem.189.11.1783, Yoshihara, E. et al. Front Immunol 2014, 4, 514-514. https://doi.org/10.3389/fimmu.2013.00514).
The disease-correlated nature of these dysregulated states of oxidoreductases—particularly of Trx, TrxR, and PDIs—makes them promising targets for diagnostic or therapeutic applications using diagnostic or therapeutic drugs that target these oxidoreductases. For example, the TrxR-inhibiting drug auranofin is FDA-approved for use in the inflammatory disease, rheumatoid arthritis (Lee, S. et al. Antioxid Redox Signal 2013, 18, 1165-1207. https://doi.org/10.1089/ars.2011.4322; Arner, E. S. J. et al. Seminars in Cancer Biology 2006, 16, 420-426. https://doi.org/10.1016/j.semcancer.2006.10.009). In this context, a probe or prodrug system to release a molecular cargo, that is selectively triggered by one of these oxidoreductases, would be very valuable. Similarly, substantial efforts have been made to use the disease-correlated feature, hypoxia, both as a diagnostic marker and for therapeutic exploitation, for example by using diagnostic and therapeutic drugs that target hypoxia, which has been extensively reviewed (Airley, R. et al. The Pharmaceutical Journal 2000, 264, 666-673; Phillips, R. M. Cancer Chemotherapy and Pharmacology 2016, 77, 441-457. https://doi.org/10.1007/s00280-015-2920-7).
Therefore it was a further object of the present invention to provide compounds which may have high selectivity for a oxidoreductase in a pathologically dysregulated state, and can be reduced by the disulfide reductive activity of that oxidoreductase or its effector proteins and thus release a molecular cargo after reduction. It was a further object of the present invention to provide compounds which may have high selectivity for pathologically dysregulated redox states and/or for cells in hypoxia and/or cells with an activated hypoxic response pathway.
It was a further object of the present invention to provide compounds which are also useful, e.g. in a diagnostic or therapeutic capacity, in that they are efficiently activated upon delivery into cells so releasing a molecular cargo, in which case the delivery into the cell is intended to be the process that gives selectivity for the targeted cells or tissues.
In conclusion therefore, the objects of the present invention were to provide compounds which are capable of releasing a molecular cargo after reductive activation, and which
Note that it is possible, and in many cases it is desirable, that one or more of these objectives should be fulfilled by the same compound; for example, a single compound of the invention may fulfil objectives A and E simultaneously; while another compound of the invention may fulfil objectives A, B, C and D simultaneously, etc.
The present invention relates to the following items:
A-L-B (I)
Unless stated otherwise the following definitions apply.
The term “alkyl” refers to a saturated straight or branched hydrocarbon chain. In any of the below definitions, C1-4-alkyl is not particularly limited, and refers to a saturated straight or branched hydrocarbon chain, which has 1 to 4 carbon atoms, preferably 1 to 3 carbon atoms, more preferably 1 or 2 carbon atoms, even more preferably 1 carbon atom. Preferably C1-4-alkyl can be methyl, ethyl, n-propyl, or i-propyl, more preferably it can be methyl or ethyl, most preferably it can be methyl.
In any of the below definitions, C2-4-alkylene is not particularly limited, and refers to a saturated straight or branched hydrocarbon chain, which has 2 to 4 carbon atoms, preferably 2 or 3 carbon atoms, more preferably 2 carbon atoms. Preferably C2-4-alkylene can be ethylene, or n-propylene, more preferably it can be ethylene.
An aromatic ring is any not particularly limited and refers to any aromatic carbocyclic ring, which has 5 to 7 carbon atoms, preferably 5 or 6 carbon atoms, more preferably the aromatic ring is phenyl. The aromatic ring can optionally be annulated to one or more carbocyclic or heterocyclic rings. Examples of annulated rings include but are not limited to naphthyl, quinolyl, isoquinolyl, quinoxalyl, benzopyranonyl, benzo[1,3]dioxolyl, benzothiazolyl, 2,3-dihydro-1H-benzo[e]indole, xanthenyl or indolyl, preferably naphthyl, quinolyl or xanthenyl.
The heteroaromatic ring is any not particularly limited and refers to any aromatic heterocyclic ring, which has 5 to 7 ring atoms, preferably 5 or 6 ring atoms. The heteroaromatic ring can optionally be annulated to one more carbocyclic or heterocyclic rings. Examples of the heteroaromatic ring include thiophenyl, pyridinyl, pyrazolyl, pyrimidinyl, purinyl, pyrrolo, furanyl, oxazolyl, thiazolyl, imidazolyl, benzofuranyl, benzothiophenyl, benzothiazolyl, benzoxazolyl or naphthyridinyl, preferably pyrrolo, pyridinyl, thiophenyl or furanyl.
An aliphatic moiety is a saturated or unsaturated, linear, branched or cyclic moiety containing between 1 to 50 carbon atoms and optionally 0 to 19 heteroatoms, preferably 0 to 15 heteroatoms, wherein the heteroatoms are typically chosen from O, N, S, Se, Si, Hal, B or P, preferably chosen from O, N, Hal, S, Si or P, more preferably O, N, Hal, S, Si, even more preferably chosen from O, N or Hal.
A carbocyclic ring is a cyclic structure containing from 4 to 50 carbon atoms. Examples of the carbocyclic ring include cyclobutane, cyclopentane, cyclohexane, benzene, cycloheptane, naphthalene or anthracene, preferably cyclohexane, cyclopentane, benzene or naphthalene, more preferably benzene or naphthalene.
A heterocyclic ring is a cyclic structure containing at least one heteroatom selected from N, O, Si, S, B, P, and Se, and containing at least one carbon atom. Examples of the heterocyclic ring include thiophene, pyridine, pyrazole, pyrimidine, pyrrole, furan, oxazole, thiazole, imidazole, quinoline, isoquinoline, benzofuran, benzothiophene, benzothiazole, benzoxazole, naphthyridine, piperidine, piperazine, pyrrolidine, tetrahydrothiophene, tetrahydrofuran or pyran, preferably pyridine, furan, thioazole, quinoline, isoquinoline, benzothiazole, piperidine, piperazine or pyran, more preferably pyridine, quinoline, piperidine or piperazine.
Halogen refers to —F, —Cl, —Br or —I, preferably —F or —Cl.
If a moiety is referred to as being “optionally substituted” by a substituent it can in each instance include one or more of the indicated substituents.
Disulfide reductase (also referred to as disulfide oxidoreductase) refers to an enzyme capable of reducing disulfides to the corresponding thiols. Examples thereof include thioredoxin reductase 1 (TrxR1), TrxR2, thioredoxin glutathione reductase (TrxR3/TGR), endoplasmic reticulum resident protein 18 (ERp18), ERp44, ERp57, ERp72, ERdj5, methionine-R-sulfoxide reductase (MsrB1), protein disulfide isomerase A 1 (PDIA1), PDIA2, PDIA3, PDIA4, PDIA5, PDIA6, EndoPDI, PDIp, disulfide bond formation protein A (DsbA), DsbB, DsbC, or glutathione disulfide reductase (GR). A disulfide effector protein (or redox effector protein or simply effector protein) is a redox active protein, that bears a reducing thioredoxin-like domain and that is itself reductively enabled by an upstream disulfide oxidoreductase enzyme. Examples thereof include thioredoxin 1 (Trx1), Trx2, thioredoxin-related protein of 14 kDa (TRP14), thioredoxin-related transmembrane protein 1 (TMX1), TMX2, TMX3, TMX4, thioredoxin-like protein 1 (TXNL1), human macrothioredoxin (hMTHr), anterior gradient protein 2 (AGR2), AGR3, glutaredoxin 1 (Grx1), Grx2, Grx3, Grx4, Grx5, GrxA or Grx6.
The term “cancer”, as used herein, refers to a group of proliferative diseases characterized by uncontrolled division of abnormal cells in a subject. Non-limiting examples of cancers include acoustic neuroma, adenocarcinoma, angiosarcoma, basal cell carcinoma, bile duct carcinoma, bladder carcinoma, breast cancer, bronchogenic carcinoma, cervical cancer, chondrosarcoma, chordoma, choriocarcinoma, craniopharyngioma, cystadenocarcinoma, embryonal carcinoma, endotheliosarcoma, ependymoma, epithelial carcinoma, Ewing's tumor, fibrosarcoma, hemangioblastoma, leiomyosarcoma, liposarcoma, Merkel cell carcinoma, melanoma, mesothelioma, myelodysplastic syndrome, myxosarcoma, oligodendroglioma, osteogenic sarcoma, ovarian cancer, pancreatic cancer, papillary adenocarcinomas, papillary carcinoma, pinealoma, prostate cancer, renal cell carcinoma, retinoblastoma, rhabdomyosarcoma, sebaceous gland carcinoma, seminoma, squamous cell carcinoma, sweat gland carcinoma, synovioma, testicular tumor, Wilms' tumor, adrenocortical carcinoma, urothelial carcinoma, gallbladder cancer, parathyroid cancer, Kaposi sarcoma, colon carcinoma, gastrointestinal stromal tumor, anal cancer, rectal cancer, small intestine cancer, brain tumor, glioma, glioblastoma, astrocytoma, neuroblastoma, medullary carcinoma, medulloblastoma, meningioma, leukemias including acute myeloid leukemia, multiple myeloma, acute lymphoblastic leukemia, liver cancer including hepatoma and hepatocellular carcinoma, lung carcinoma, non-small-cell lung cancer, small cell lung carcinoma, lymphangioendotheliosarcoma, lymphangiosarcoma, primary CNS lymphoma, non-Hodgkin lymphoma, and classical Hodgkin's lymphoma.
The term “cargo”, as used herein, represents a therapeutic, diagnostic or theranostic agent to be delivered to a site of enzymatic activity or disease (e.g. a tumor, a site of inflammatory or autoimmune disease). Cargos used in the compounds of the invention may be small molecules. Examples include agents for treating, ameliorating, preventing or diagnosing a neoplastic disorder, an inflammatory disorder (e.g. a non-steroidal anti-inflammatory drug (NSAID), a disease-modifying anti-rheumatic drug (DMARD), or mesalamine), atherosclerosis; an autoimmune disorder; a chronic inflammatory autoimmune disorder; ischaemia; and reperfusion injury as well as kinase inhibitors. Examples also include diagnostically acceptable dyes, therapeutically acceptable DNA-alkylating agents, therapeutically acceptable DNA-intercalating agents or therapeutically acceptable tubulin-inhibiting agents.
Non-limiting examples of antineoplastic agents include 10-hydroxycamptothecin, 10-hydroxybelotecan, 10-hydroxygimatecan, 10-hydroxy-CKD-602, 10-hydroxy-BNP-1350, 10-hydroxy-sinotecan, topotecan, 7-ethyl-10-hydroxy-camptothecin (SN-38), 10-hydroxy-20-acetoxy-camptothecin, pyrrolobenzodiazepine, methotrexate, duocarmycin, CC-1065, doxorubicin, epirubicin, daunorubicin, pirarubicin, carminomycin, doxorubicin-N,O-acetal, 4-(bis(2-chloroethyl)amino)phenol, 4-(bis(2-bromoethyl)amino)phenol, 4-(bis(2-mesylethyl)-amino)phenol, 4-((2-chloroethyl-2′-mesylethyl)amino)phenol, (S)-(1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indol-3-yl)(5,6,7-trimethoxy-1H-indol-2-yl)methanone, (S)-(5-(3-(azetidin-1-yl)propoxy)-1H-indol-2-yl)(1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo-[e]indol-3-yl)methanone, 5-hydroxy-seco-cyclopropabenzaindoles, 5-hydroxy-seco-(2-methyl-cyclopropa)benzaindoles, 5-hydroxy-seco-cyclopropamethoxybenzaindoles, 5-amino-seco-cyclopropabenzaindoles, etoposide, teniposide, GL331, NPF, TOP53, NK611, tubulysin A, tubulysin B, tubulysin C, tubulysin G, tubulysin I, monomethyl auristatin E, monomethyl auristatin F, dolastatin 10, dolastatin 15, symplostastin 1, symplostastin 3, narciclasine, pancratistatin, 2-epi-narciclasine, narciprimine, calicheamicin α1, calicheamicin β1, calicheamicin γ1, calicheamicin δ1, calicheamicin ε, calicheamicin θ, or calicheamicin T.
Non-limiting examples of NSAIDs include diclofenac, aceclofenac, mefenamic acid, clonixin, piroxicam, meloxicam, tenoxicam, or lornoxicam.
Non-limiting examples of DMARDs include methotrexate.
Non-limiting examples of kinase inhibitors include baricitinib, filgotinib, tofacitinib, upadacitinib, ruxolitinib, peficitinib, decemotinib, solcitinib, itacitinib, fostamatinib, or SHRO302. Particularly of interest are inhibitors of the Janus Kinase (JAK) family of kinases, such as of JAK1 or JAK3.
Cargos used in the compounds of the invention may be diagnostic agents (e.g., a fluorescent agent or a radiotracer agent), that may be used to detect and/or determine the stage of a targeted pathology such as a tumor, or to measure or localise redox activity. Non-limiting examples of fluorescent diagnostic agents include leuco-methylene blue, leuco-methyl methylene blue, leuco-dimethyl methylene blue, leuco toluidine blue, leuco-Azure A, leuco-Azure B, leuco-Azure C, leuco-Thionin, leuco-methylene violet, leuco-new methylene blue, leuco-Nile blue A, leuco-brilliant cresyl blue, firefly luciferin (D-Luciferin), umbelliferone, 4-trifluoromethylumbelliferone, 6,8-difluoro-4-methylumbelliferone, 7-hydroxycoumarin-3-carboxylic acid, 6,8-difluoro-7-hydroxy-5-methylcoumarin (DiFMU), 7-amino-4-methylcoumarin, 7-amino-4-chloromethylcoumarin, 3-O-methylfluorescein, 3-O-ethyl-5-carboxyfluorescein, 2,7-difluoro-3-O-methylfluorescein, 3-N-acetyl-rhodamine, 3-N-acetyl-dimethylsilarhodamine, 2,7-dibromo-3-N-acetyl-dimethylcarborhodamine, 3-N-acetyl-6-carboxyrhodamine, 2,7-difluoro-3-N-acetylrhodol, 3-O—(N,N-dimethyl-2-aminoethyl)-6-carboxyfluorescein, 2,7-dichloro-3-O—(N,N-dimethyl-2-aminoethyl)fluorescein, blackberry quencher (BBQ), black hole quencher 3 (BHQ3), 2-(2-hydroxyphenyl)quinazolin-4-one, 6-chloro-2-(5-chloro-2-hydroxyphenyl)quinazolin-4-one, or 6-bromo-2-(5-bromo-2-hydroxyphenyl)quinazolin-4-one.
Radiotracer agents are agents useful for imaging which incorporate a radioactive isotope, non-limiting examples of which are radioactive isotopes of carbon, cobalt, fluorine, gallium, iodine, or technetium, preferably carbon, fluorine or iodine. Compounds of the present invention which have been labelled with a radioactive isotope can be employed as diagnostic applications.
The term “pharmaceutically acceptable salt” refers to a salt of a compound of the present invention. Suitable pharmaceutically acceptable salts include acid addition salts which may, for example, be formed by mixing a solution of compounds of the present invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulfuric acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, citric acid, tartaric acid, carbonic acid or phosphoric acid. Furthermore, where the compound carries an acidic moiety, suitable pharmaceutically acceptable salts thereof may include alkali metal salts (e.g., sodium or potassium salts); alkaline earth metal salts (e.g., calcium or magnesium salts); and salts formed with suitable organic ligands (e.g., ammonium, quaternary ammonium and amine cations formed using counteranions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, alkyl sulfonate and aryl sulfonate). Illustrative examples of pharmaceutically acceptable salts include, but are not limited to, acetate, adipate, ascorbate, aspartate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, butyrate, calcium edetate, camphorate, camphorsulfonate, camsylate, carbonate, chloride, citrate, clavulanate, cyclopentanepropionate, digluconate, dihydrochloride, dodecylsulfate, edetate, edisylate, estolate, esylate, ethanesulfonate, formate, fumarate, gluceptate, glucoheptonate, gluconate, glutamate, glycerophosphate, hemisulfate, heptanoate, hexanoate, hydrabamine, hydrobromide, hydrochloride, hydroiodide, 2-hydroxy-ethanesulfonate, hydroxynaphthoate, iodide, isothionate, lactate, lactobionate, laurate, lauryl sulfate, malate, maleate, malonate, mandelate, mesylate, methanesulfonate, methylsulfate, mucate, 2-naphthalenesulfonate, napsylate, nicotinate, nitrate, N-methylglucamine ammonium salt, oleate, oxalate, pamoate (embonate), palmitate, pantothenate, pectinate, persulfate, 3-phenylpropionate, phosphate/diphosphate, picrate, pivalate, polygalacturonate, propionate, salicylate, stearate, sulfate, succinate, tannate, tartrate, teoclate, tosylate, triethiodide, undecanoate, valerate, and the like (see, for example, S. M. Berge et al.; J. Pharm. Sci. 1977, 66, 1-19).
When the compounds of the present invention are provided in crystalline form, the structure can contain solvent molecules. The solvents are typically pharmaceutically acceptable solvents and include, among others, water (hydrates) or organic solvents. Examples of possible solvates include hydrates, ethanolates and iso-propanolates.
The term “pharmaceutically acceptable ester” refers to an ester of a compound of the present invention. Suitable pharmaceutically acceptable esters include acetyl, butyryl, and acetoxymethyl esters.
The term “protecting group”, as used herein (represented in schemes as moieties -PG), represents a group intended to protect a hydroxy or an amino group from participating in one or more undesirable reactions during chemical synthesis. The term “O-protecting group”, as used herein, represents a group intended to protect a hydroxy or carbonyl group from participating in one or more undesirable reactions during chemical synthesis. The term “N-protecting group”, as used herein, represents a group intended to protect a nitrogen containing (e.g., an amino or hydrazine) group from participating in one or more undesirable reactions during chemical synthesis. Exemplary O- and N-protecting groups include, but are not limited to: alkanoyl, aryloyl, or carbamyl groups such as formyl, acetyl, propionyl, pivaloyl, tert-butylacetyl, 2-chloroacetyl, 2-bromoacetyl, trifluoroacetyl, trichloroacetyl, phthalyl, o-nitrophenoxyacetyl, α-chlorobutyryl, benzoyl, 4-chlorobenzoyl, 4-bromobenzoyl, triiso-propylsilyloxymethyl, 4,4′-dimethoxytrityl, isobutyryl, phenoxyacetyl, 4-isopropylpehenoxyacetyl, dimethylformamidino, and 4-nitrobenzoyl. Other O-protecting groups include, but are not limited to: substituted alkyl, aryl, and aryl-alkyl ethers (e.g., trityl; methylthiomethyl; methoxymethyl; benzyloxymethyl; siloxymethyl; 2,2,2,-trichloroethoxymethyl; tetrahydropyranyl; tetrahydrofuranyl; ethoxyethyl; 1-[2-(trimethylsilyl)ethoxy]ethyl; 2-trimethylsilylethyl; tert-butyl ether; p-chlorophenyl, p-methoxyphenyl, p-nitrophenyl, benzyl, p-methoxybenzyl, and nitrobenzyl); silyl ethers (e.g., trimethylsilyl; triethylsilyl; triisopropylsilyl; dimethylisopropylsilyl; tert-butyldimethylsilyl; tert-butyldiphenylsilyl; tribenzylsilyl; triphenylsilyl; and diphenymethylsilyl); carbonates (e.g., methyl, methoxymethyl, 9-fluorenylmethyl; ethyl; 2,2,2-trichloroethyl; 2-(trimethylsilyl)ethyl; vinyl, allyl, nitrophenyl; benzyl; methoxybenzyl; 3,4-dimethoxybenzyl; and nitrobenzyl). Other N-protecting groups include, but are not limited to, chiral auxiliaries such as protected or unprotected, L- or D-amino acids such as alanine, leucine, phenylalanine, and the like; sulfonyl-containing groups such as benzenesulfonyl, p-toluenesulfonyl, and the like; carbamate forming groups such as benzyloxycarbonyl, p-chlorobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, 2-nitrobenzyloxycarbonyl, p-bromobenzyloxycarbonyl, 3,4-dimethoxybenzyloxycarbonyl, 3,5-dimethoxybenzyl oxycarbonyl, 2,4-dimethoxybenzyloxycarbonyl, 4-methoxybenzyloxycarbonyl, 2-nitro-4,5-dimethoxybenzyloxycarbonyl, 3,4,5-trimethoxybenzyloxycarbonyl, 1-(p-biphenylyl)-Imethylethoxycarbonyl, a,a-dimethyl-3,5-dimethoxybenzyloxycarbonyl, benzhydryloxy carbonyl, tbutyloxycarbonyl, diisopropylmethoxycarbonyl, isopropyloxycarbonyl, ethoxycarbonyl, methoxycarbonyl, allyloxycarbonyl, 2,2,2,-trichloroethoxycarbonyl, phenoxycarbonyl, 4-nitrophenoxy carbonyl, fluorenyl-9-methoxycarbonyl, cyclopentyloxycarbonyl, adamantyloxycarbonyl, cyclohexyloxycarbonyl, phenylthiocarbonyl, and the like, aryl-alkyl groups such as benzyl, triphenylmethyl, benzyloxymethyl, and the like and silyl groups such as trimethylsilyl, and the like. Useful N-protecting groups are formyl, acetyl, benzoyl, pivaloyl, tert-butylacetyl, alanyl, phenylsulfonyl, benzyl, tert-butyloxycarbonyl (Boc), and benzyloxycarbonyl (Cbz).
The term “proteinogenic amino acids” herein comprises the 20 principal naturally-occurring L-amino acids (glycine, alanine, valine, leucine, isoleucine, phenylalanine, proline, methionine, serine, threonine, cysteine, tyrosine, tryptophan, asparagic acid, glutamic acid, asparagine, arginine, histidine, lysine, asparagine and glutamine) which are to be attached, as the monopeptide, via their carboxyl terminus to the specified amine nitrogen of the compound, creating amides. Preferably the proteinogenic amino acid is selected from leucine, serine and lysine.
The term “self-immolative spacer”, also called “traceless linker” or “self-immolative linker”, as used herein, represents a multivalent (e.g., divalent) group covalently linking a disulfide-containing group which will be further defined below (as the moiety A), to a cargo B, in such a way that reductive cleavage of the disulfide bond in the moiety A is followed by a sequence of reactions which result in the cleavage of a bond between the self-immolative linker group and the cargo B, thereby releasing the cargo. Illustrative examples thereof include:
The stereochemistry at the ring atoms bridging the two rings of the bicyclic disulfide structure of the compounds of formula (I) of the invention is not particularly limited; any absolute configuration of the two bridging ring tertiary carbon atoms is within the scope of the compounds of formula (I). Either carbon can be separately either R, or S, or a mixture of R and S in any proportions (including racemic and undefined proportions); therefore the disulfide ring may be considered to be cis-fused to the carbamate-containing ring with any given stereochemistry, or trans-fused to the carbamate-containing ring with any given stereochemistry, or present as a mixture in any proportions of cis-fused and trans-fused with any given stereochemistries (including racemic and undefined proportions). Preferably, the disulfide ring is either cis-fused or trans-fused.
The term “vascular disrupting agent” refers to a compound that reduces tumor blood flow upon administration. Such agents are typically microtubule-depolymerising agents, and are well represented in the literature (Gill, Pharmacology & Therapeutics 2019, 202, 18-31); as known to those skilled in the art, these include combretastatin A-4 (CA4), 3′-aminocombretastatin A-4, BNC105, ABT-751, ZD6126, combretastatin A-1, and prodrugs of the same, including but not limited to combretastatin A-4 phosphate (CA4P), 3′-aminocombretastatin A-4 3′-serinamide (ombrabulin), combretastatin A-1 bisphosphate (CA1P), and BNC105 phosphate (BNC105P).
The term “cytotoxic chemotherapeutic agent” refers to compounds that have antineoplastic and/or antitumoral efficacy and which are typically useful in the treatment of cancer, optionally as part of combination therapies. Such agents include paclitaxel, docetaxel, nab-paclitaxel, epothilone, mertansine, irinotecan, etoposide, teniposide, mitoxantrone, doxorubicin, daunorubicin, epirubicin, cisplatin, carboplatin, oxaliplatin, melphalan, chlorambucil, busulfan, methotrexate, permetrexed, cyclophosphamide, 5-fluorouracil, capecitabine, gemcitabine, cytarabine, fludarabine, mercaptopurine, vinolrebine, vinblastine, vincristine, dolastatin, monomethyl auristatin E, monomethyl auristatin F, bleomycin, dacarbazine, pyrrolobenzodiazepine, temozolomide, carmustine, mitomycin and procarbazine.
In the context of the invention the term “immunomodulator” refers to therapeutically active compounds that interact with the immune system, which may for example be useful in the treatment of cancer or autoimmune diseases, optionally as part of combination therapies. Such agents include anti-PD-1 antibody such as pembrolizumab and nivolumab, anti-PD-L1 antibody such as avelumab, anti-CTLA4 antibody such as ipilimumab, cytokine agonists such as proleukin and interferon alfa-2a/interferon alfa-2b, mesalamine, baricitinib, filgotinib, tofacitinib, upadacitinib, ruxolitinib, peficitinib, decemotinib, solcitinib, itacitinib, fostamatinib, and SHR0302.
The present invention provides a compound having the formula (I)
A-L-B (1)
In one embodiment, W is selected from —OH, —N(Rh)(Ri), or a heterocyclic group selected from azetidinyl, pyrrolidinyl, piperidinyl or morpholino, wherein the heterocyclic group is attached to the —C1-4-alkyl or —(C2-4-alkylene)- group via the N atom. Preferably, W is selected from —OH, —N(CH3)2, -azetidinyl or -morpholino. More preferably, W is selected from —OH and —N(CH3)2. In another embodiment, W is —C(O)-heterocyclic group, wherein the heterocyclic group is attached to the C(O) moiety via the N atom and wherein the heterocyclic group is preferably piperazinyl or N′—(C1-4-alkyl)piperazinyl (e.g., N′-(methyl)piperazinyl).
L is a bond or a self-immolative spacer. In one embodiment, L is a bond. In another embodiment, L is a self-immolative spacer. A self-immolative spacer is any linking group which is capable of covalently linking A and B and which can release the cargo H-B when the disulfide bond in A is cleaved and the resulting dithiol species cyclizes via nucleophilic attack at the carbonyl group of A. The self-immolative spacer is not particularly limited and can be chosen from any known self-immolative spacer. Examples are to be found in e.g. Blencowe, C. A. et al. Polym. Chem. 2011, 2, 773-790. https://doi.org/10.1039/COPY00324G. Preferred examples thereof include, but are not limited to, the moieties shown in the Definitions section, and, in particular, para-hydroxybenzylic 1,4-elimination self-immolative spacers and para-hydroxybenzylic 1,6-elimination self-immolative spacers:
In these formulae (R)q— can be attached at any available position on the benzene ring. and can be attached at either of the carbon atoms which are indicated by the dashed lines.
More preferably the self-immolative spacer is a para-hydroxybenzylic 1,6-elimination spacer:
In these formulae
If L is a bond then the cargo B, which is a therapeutic, diagnostic or theranostic agent, can contain an —OH group that is attached to a 5- or 6-membered aromatic or heteroaromatic ring, preferably an —OH group that is attached to a 6-membered aromatic ring, more preferably an —OH group that is attached to phenyl ring. The 5- or 6-membered aromatic or heteroaromatic ring or the phenyl ring can be part of a larger structure as defined below. In these embodiments L is suitable for binding to A via the O atom, giving a carbamate.
If L is
then the cargo, which may be a therapeutic, diagnostic or theranostic agent (such as an aniline or amine) that contains an —NH2 or —NH— moiety; or an —OH group that is attached to a 5- or 6-membered aromatic or heteroaromatic ring that can be part of a larger structure as defined below; preferably, an —OH group that is attached to a 5- or 6-membered aromatic ring that can be part of a larger structure as defined below; more preferably, an —OH group that is attached to a phenyl ring that can be part of a larger structure as defined below.
In these embodiments the —NH2 or —NH— moiety or the OH group is suitable for binding to L via the respective N or O atom.
If L is
then the cargo, which is a therapeutic, diagnostic or theranostic agent, can contain an —NH2 or —NH— moiety; or an —OH group that is attached to a 5- or 6-membered aromatic or heteroaromatic ring; preferably an —NH2 or —NH— moiety which is attached to an aromatic ring that can be part of a larger structure as defined below, or an —OH group that is attached to a 5- or 6-membered aromatic ring that can be part of a larger structure as defined below; more preferably an —OH group that is attached to a phenyl ring that can be part of a larger structure as defined below.
In these embodiments the —NH2 or —NH— moiety or the OH group is suitable for binding to L via the respective N or O atom.
Each group R is independently selected from -halogen —O(Rr), —N(Rs)(Rt), —NO2, —CN, and a heterocyclic group selected from azetidinyl, pyrrolidinyl, piperidinyl or morpholino, wherein the heterocyclic group is attached to the aromatic ring via the N atom. Preferably, group R is a halogen (such as F or Cl) in ortho position relative to the bond to A; also preferably, group R is —O(Rr) in ortho position relative to the bond to B.
Rr is selected from —H and —C1-4-alkyl, preferably —C1-4-alkyl, more preferably Rr is methyl.
Rs is selected from —H, —C(O)—C1-4-alkyl and —C1-4-alkyl, preferably —H or —C1-4-alkyl, more preferably —H or —CH3.
Rt is selected from —H, —C(O)—C1-4-alkyl and —C1-4-alkyl, preferably —H or —C1-4-alkyl, more preferably —H or —CH3.
Most preferred examples of self-immolative spacers that link groups A and B are
If A2 and A3 are bound together to form a carbocyclic or heterocyclic ring, the carbocyclic or heterocyclic ring can contain from 3 to 50 carbon atoms, preferably from 5 to 50 carbon atoms, more preferably 6 to 50 carbon atoms. The hydrocarbon moiety can optionally contain 1 or more heteroatoms, preferably 1 to 20 heteroatoms, more preferably 1 to 15 heteroatoms, wherein the heteroatoms can be selected from O, N, S, Se, Si, Hal, B or P, preferably selected from O, N, Hal, S, Si or P, more preferably selected from O, N, Hal, S, Si, even more preferably selected from O, N or Hal.
The nature of the therapeutic agent, diagnostic agent or theranostic agent is not particularly limited, as long as it contains an —OH, —NH2 or —NH— moiety which after removal of the H is suitable for binding to the self-immolative spacer L, or, if L is a bond, to A, via the O or N atom. As can be seen from the schemes below, the O or N atom partakes in the reaction when the moiety A of a compound having the formula (I) is cleaved by a disulfide reductase or its effector disulfide protein. The rest of the therapeutic agent, diagnostic agent or theranostic agent is not involved in this reaction, so that a wide variety of therapeutic agents, diagnostic agents and theranostic agents are suitable for use in the present invention.
Examples of suitable therapeutic agents, diagnostic agents and theranostic agents include, but are not limited to diagnostically acceptable dyes, therapeutically acceptable DNA-alkylating agents, therapeutically acceptable DNA-intercalating agents or therapeutically acceptable tubulin-inhibiting agents. In one embodiment, diagnostically acceptable dyes are preferred, which includes compounds or structurally and functionally related derivatives selected from, but not limited to the following classes of dyes: xanthenes including e.g. alkyl-fluoresceins, alkyl-carbofluoresceins, alkyl-silarhodamines, phenylquinazolinones, coumarins, firefly-luciferins, black hole quencher dyes, or any compound derived by substitution of the same. How acceptable compounds may be derived by substitution will be clarified in the subsequent examples. In another embodiment, therapeutically acceptable agents are preferred, which includes theranostic agents that include structurally and functionally related derivatives of the leuco-methylene blue class; DNA-alkylating agents that include compounds or structurally and functionally related derivatives selected from, but not limited to the following classes: nitrogen mustards or seco-cyclopropabenzaindoles; DNA-intercalating agents that include compounds or structurally and functionally related derivatives selected from, but not limited to the following classes of compounds: anthracyclins or camptothecins; antiproliferative agents that include compounds or structurally and functionally related derivatives selected from, but not limited to the following classes: monomethyl auristatins, the Amaryllidaceae alkaloids; or any compounds derived by substitution of the same.
Examples of therapeutic agents, diagnostic agents and theranostic agents include, but are not limited to, agents for treating, ameliorating, preventing or diagnosing a neoplastic disorder, an inflammatory disorder (e.g. a non-steroidal anti-inflammatory drug (NSAID), a disease-modifying anti-rheumatic drug (DMARD), or mesalamine), atherosclerosis; an autoimmune disorder; a chronic inflammatory autoimmune disorder; ischaemia; and reperfusion injury as well as kinase inhibitors.
Preferred are agents for which the conjugation of the active agent H-B into the compound A-L-B results in a substantial reduction of diagnostic signal or therapeutic potency; particularly preferred are therapeutically acceptable agents for which the conjugation of the active agent H-B into the compound A-L-B totally or near-totally prevents the compound from exhibiting the diagnostic signal or therapeutic potency that is associated with the free agent H-B. This preferred total or near-total prevention of the activity of agent H-B when conjugated as A-L-B is possible in several ways, non-limiting examples of which are given below in the section Utility, Diseases, and Examples.
How suitable diagnostic, therapeutic or theranostic agents may be derived by substitution of specific agents will be understood by reference to the following non-limiting examples: A1-OH or A2-NH-A3 can be a diagnostic agent of the xanthene class:
When A1 is selected such that A1-OH is a diagnostic agent of the phenylquinazolinone class:
One or more substituent L6 and one or more substituent L7 are independently selected from —H, —C1-4-alkyl, -halogen, —O—C1-4-alkyl, —CF3, —NO2.
When A1 is selected such that A1-OH is a diagnostic agent of the umbelliferone class:
When A1, A2 or A3 are selected such that A1-OH or A2-NH-A3 is a therapeutic agent of the seco-cyclopropabenzaindole (CBI) class of DNA-alkylating anti-cancer drugs:
One or more substituent L11 is independently selected from —H, —F, —C1-4-alkyl, —CF3, —OMe, —O—C1-4-alkyl.
When A2 and A3 are selected such that A2-NH-A3 is a diagnostic or theranostic agent of the leuco-methylene blue class:
When A1 is selected such that A1-OH is a therapeutic agent of the camptothecin class of DNA-intercalating anti-cancer drugs:
One or more substituent L18 are independently selected from —H, —F, —CF3, —CH2—CF3, —C1-4-alkyl, —CH2—N(—C1-4-alkyl)2.
When A1 or A2 and A3 are selected such that A1-OH or A2-NH-A3 is a therapeutic agent of the nitrogen mustard class of DNA-alkylating agents:
When A1 is selected such that A4-OH is a diagnostic agent of the firefly luciferin class:
One or more substituent L23 is selected from —H, —C1-4-alkyl, -halogen, —O—C1-4-alkyl, —CF3, —NO2.
When A1 is selected such that A1-OH is a therapeutic agent of the Amaryllidaceae class of anti-proliferative alkaloids:
When A1 is selected such that A1-OH is a diagnostic agent of the Black hole quencher class:
One or more substituent L25 is independently selected from —H, —C1-4-alkyl, -halogen, —O—C1-4-alkyl, —CF3, —NO2.
When A2 and A3 are selected such that A2-NH-A3 is a therapeutic agent of the anthracycline class:
If J8 is selected from H, —C1-4-alkyl, —OH, —O-(tetrahydro-2H-pyran-2-yl), —NH2, —O—C1-4-alkyl, —CF3, then J9 is selected from —CH2—, —C(—C1-4-alkyl)2-.
If J8 is selected from —CH2—, —C(—C1-4-alkyl)2-, —O—, —S—, then J9 is selected from —CH2—, —C(—C1-4-alkyl)2-.
One or more substituent L29 is independently selected from —O—C1-4-alkyl, —OH, -halogen, —NH2, —O—C1-4-alkyl, —CF3.
When A2 and A3 are selected such that A2-NH-A3 is a therapeutic agent of the auristatin class:
Illustrative embodiments of the compound having the formula (I) include
Illustrative embodiments of the compound having formula (I) also include
It is understood that all combinations of the above definitions and preferred definitions are envisaged by the present inventors.
In some embodiments, general manufacturing of the compound of the invention can begin from a diol precursor similar to diol A1, which itself can be prepared from commercially available starting materials using literature known procedures.
PG1 represents any protecting group for the heterocyclic nitrogen atom, which intermediately masks the nitrogen's nucleophilic reactivity and can be cleaved without affecting other functional groups in the molecule. Preferably PG1 can be selected from any of the following N-protecting groups: acetamide (Ac), trifluoroacetamide, tert-butyl carbamate (Boc), benzyl carbamate (Cbz), trityl (Trt), dimethoxy-trityl (DMT), toluenesulfonyl (Ts), o-nitro benzenesulfonyl (Ns), 9-fluorenmethyl carbamate (Fmoc), p-methoxy benzyl carbamate (Moc), benzoyl (Bz), 3,4-dimethoxybenzyl (DMPM), p-methoxybenzyl (PMB), p-methoxyphenyl (PMP), and trichloroethyl carbamate (Troc). More preferably PG1 can be selected from one of the following N-protecting groups with preferred compatibility: acetamide (Ac), trifluoroacetamide, tert-butyl carbamate (Boc), trityl (Trt), dimethoxy-trityl (DMT), 9-fluorenmethyl carbamate (Fmoc), and trichloroethyl carbamate (Troc). Most preferably PG1 can be selected from of following N-protecting groups with preferred compatibility and accessibility: acetamide (Ac), and tert-butyl carbamate (Boc).
Oxo-sulfur substitution can be generally achieved by any substitution reaction using any sulfur nucleophile and any activation method for alcohols. Preferably, oxo-sulfur substitution can be achieved by using (1) one of the following nucleophiles: thioacetic acid (HSAc) or its sodium/potassium salt (Na/KSAc), thiobenzoic acid (HSBz) or its sodium/potassium salt (Na/KSBz), p-nitro-thiobenzoic acid or its sodium/potassium salt, tert-butyl mercaptan (HS-tBu), benzyl mercaptan (HS-Bn), p-methoxybenzyl mercaptan (HS-PMB), methanethiol (HS-Me) or any alkyl thiol (HS-alkyl); and (2) one of the following activation reagents: p-toluenesulfonyl chloride (TsCl), methanesulfonyl chloride (MsCl), trifluoromethanesulfonic anhydride (Tf2O), dialkyl azodicarboxylate activated with a trialkyl- or triarylphosphine (Mitsunobu conditions: e.g. PPh3/DIAD), tetrahalogenmethane activated with a trialkyl- or triarylphosphine (PPh3/CBr4). More preferably oxo-sulfur substitution can be achieved by using mesylation/tosylation followed by potassium thioacetate in two separate steps or using Thio-Mitsunobu conditions (PPh3/DIAD followed by thioacetic acid, thiobenzoic acid, or p-nitro-thiobenzoic acid in one step). Most preferably oxo-sulfur substitution can be achieved by using Thio-Mitsunobu conditions of PPh3/DIAD followed by thioacetic acid in one step.
Depending on the selected conditions for oxo-sulfur substitution PG2 can be selected from the following sulfur protecting groups: acetate (Ac), benzoate (Bz), p-nitro benzoate, tert-butyl, methyl, alkyl or p-methoxy benzyl. More preferably, PG2 can be selected from the following sulfur protecting groups: acetate (Ac), benzoate (Bz), p-nitro benzoate. Most preferably, PG2 is acetate (Ac).
Deprotection of A2 can generally be achieved applying any appropriate deprotection conditions, which depending on PG1 and/or PG2 may be selected from the following list of general deprotection conditions: (1) acidic deprotection: solutions of HCl in organic solvents (HCl/MeOH, HCl/EtOH, HCl/dioxane, HCl/Et2O), or carboxylic acids (trifluoroacetic acid (TFA), trichloroacetic acid); (2) basic deprotection: piperidine, NaOH, KOH, Na2CO3, K2CO3, or Cs2CO3; (3) oxidative deprotection: 1,2-dichloro-4,5-dinitro-quinone (DDQ), cerium(IV) ammonium nitrate (CAN), tetrapropylammonium perruthenate, potassium perruthenate; (4) reductive deprotection: hydrogenolysis (H2 in presence of Pd/C), diisobutylaluminium hydride (DIBAL-H), Na/NH3, Li/naphthalene, Zn/HCl, LiAlH4, NaBH4, NaCNBH3. Preferred conditions are: (1) acidic deprotection: solutions of HCl in organic solvents (HCl/MeOH, HCl/EtOH, HCl/dioxane, HCl/Et2O) or carboxylic acids (trifluoroacetic acid (TFA), trichloroacetic acid); (2) basic deprotection: piperidine, KOH, or K2CO3. More preferably, acidic deprotection conditions are HCl/dioxane and basic deprotection conditions are KOH.
Deprotection conditions depending on PG1 and/or PG2 can be selected, so that PG1 and PG2 are cleaved consecutively giving the free dithiol A3 with a free amine, or so that only PG2 is cleaved giving the free dithiol A3 while leaving the N-protecting group PG1 intact.
Oxidative ring closure (formation of the disulfide) can be accomplished by any 2-electron oxidation conditions suitable for dithiol/disulfide oxidation to give disulfide A4. Preferably these are selected from the following reaction conditions: I2/MeOH, I2/EtOH, I2/DCM, O2/MeOH, O2/EtOH, O2/DCM, DMSO. More preferably these are selected from the following conditions: I2/MeOH or I2/DCM.
Deprotection of A4 can generally be achieved applying any appropriate deprotection conditions to give A5, which depending on PGi may be selected from the List of General Deprotection Conditions outlined above.
A5 is obtained as a free amine or any acceptable ammonium salt of the same which may be selected from, but not limited to the following: acetate, benzoate, bromide, chloride, formate, iodide, nitrate, sulfate, or trifluoroacetate salt.
In other embodiments, general manufacturing of the invention can begin from a diol precursor similar to diol A32, which itself can be prepared from commercially available starting materials using literature known procedures. The four steps of oxo-sulfur substitution to yield A33, deprotection to yield A34, oxidative ring closure to yield A35, and deprotection to yield A36, can proceed by the reagents and conditions outlined above.
Reductive amination of A36 can generally be achieved by any appropriate conditions for reductive amination. Depending on the nature of X3 and Y, 6-membered piperazine or 7-membered 1,4-diazepane ring structures can be prepared by applying the respective optionally substituted dialdehyde, diketone, or ketoaldehyde precursors, which may be selected, but are not limited to the following: glyoxal, malondialdehyde, diacetyl and pentane-2,4-dione, or technically acceptable formulations which release them in situ. For reduction of the intermediate cyclic diimine structure any reducing agent may be used. Preferably, a reducing agent may be selected from but is not limited to: LiAlH4, LiBH4, NaBH4, NaCNBH3, NaBH(OAc)3, KBH4, LiBHEt3 or Ti(BH4)4(OEt2). More preferably the cyclic diimine structure may be reduced using NaBH4 or NaCNBH3, most preferably using NaCNBH3.
Monofunctionalisation of A37 to achieve A38 may be achieved by any N-alkylation, N-acylation or N-sulfonylation reaction with appropriate reagents, optionally with additional use of protecting groups, as necessary, and as known to those skilled in the art. Depending on the nature of Ra, A37 may be treated with commercially available reagents, including but not limited to the following: For acylation: acetyl chloride, pivaloyl chloride, iPrC(O)—Cl, nBuC(O)Cl, methyl chloroformate, dimethyl carbamoylchloride; for sulfonylation: methanesulfonyl chloride; for alkylation: 3-(dimethylamino)propyl chloride, 3-(piperidin-1-yl)propyl chloride, 4-(azetidin-1-yl) butyl chloride, 1-(4-chlorobutyl)azetidine, 1-(3-chloropropyl)pyrrolidine, 2-(2-chloroethoxy)ethan-1-ol, methyl iodide, ethyl iodide. Depending on the nature of Rb, A37 may be coupled with a proteinogenic amino acid to prepare a monopeptide thereof. Peptide coupling may be achieved by any appropriate coupling conditions for coupling of secondary amines with carboxylic acids. Preferably, peptide coupling may be achieved by using one of the following coupling reagents: DCC, DIC, EDCl, HATU, HBTU, PyBOP or 1-hydroxybenzotriazole. Preferably, peptide coupling may be achieved by using DCC or EDCl, most preferably by using EDCl.
If PG1 is a sulfonate protecting group such as methylsulfonate (Ms), trifluoromethylsulfonate (Tf), (3-methoxyphenyl)sulfonate, (3,6-dimethoxyphenyl)sulfonate, (4-toluene)sulfonate (p-Ts), (2-nitrophenyl)sulfonate (o-Ns), (4-nitrophenyl)sulfonate (p-Ns), (2,6-dinitrophenyl)sulfonate or (2,4-dinitrophenyl)sulfonate, then ring closure of A35 may be achieved by any appropriate conditions that are used for bis-alkylation. Particularly, ring closure may be achieved by using the respective optionally substituted bis-electrophiles such as 1,2-dichloroethane, 1,2-diiodoethane, 1,2-dibromoethane, 1,2-dimesylethane, 1,2-ditosylethane or any combination of these leaving groups or an activated ethene bis-electrophile such as vinyl-thianthrenium, vinyl-dibenzothiophenium, vinyl-diphenylsulfinium or any substituted vinyl-sulfinium analog, with any counterion, preferably selected from chloride, perchlorate, nitrate, sulfate, acetate, hexafluorophosphate, hexafluoroantimonate, tetrafluoroborate, trifluoromethylsulfonate or (4-toluene)sulfonate, similar to compounds reported in Aggarwal et al., Angew. Chem. Int. Ed., 2008, 120, 3844.
Deprotection of A39 can generally be achieved applying any appropriate deprotection conditions to give A37, which depending on PG1 may be selected from the List of General Deprotection Conditions outlined above. In particular, if PG1 is a sulfonate protecting group as listed above, deprotection might be achieved by nucleophilic aromatic substitution using mild nucleophilic reagents selected from: phenol, thiophenol, alkylthiolates or alkoxides, preferably thiophenol or organic alkoxides, more preferably by using organic sodium methoxide solutions.
B) General Manufacturing: Compounds with No Self-Immolative Linker
The phenolic structure A6 represents a phenolic substructure of a motif which may be a therapeutic, diagnostic or theranostic cargo. Transformation of the phenolic alcohol function of A6 into an activated carbonate derivative may be achieved by any appropriate method for the transformation of alcohols into activated carbonate derivatives.
Methods for the transformation of alcohols into activated carbonate derivatives include using one of the following reagents: phosgene, diphosgene (DP), triphosgene (TP), 1,1′-carbodiimidazole (CDI), N,N′-disuccinimidyl carbonate (DSC), di-2-pyridyl carbonate (DPC), dimethyl carbonate (DMC), bis(p-nitrophenyl) carbonate (DNPC), bis (p-chlorophenyl) carbonate (DCPC), phenyl chloroformate, p-nitrophenyl chloroformate, p-chlorophenyl chloroformate, or ethylene carbonate with chlorine. Preferably, the reagent is DP, TP, CDI, or p-chlorophenyl chloroformate. More preferably, the reagent is TP.
LG1 depends on the selected method for the transformation of the phenolic alcohol function of A6 into an activated carbonate derivative and preferably may be one of the following: chloro, N-imidazolyl, N′-methyl-N-imidazoliumyl, succinimidyl, 2-pyridyl, methoxy, p-nitrophenyloxy, p-chlorophenyloxy, or phenyloxy. More preferably, LG1 may be one of the following: chloro, N-imidazolyl, or p-chlorophenyloxy. Most preferably, LG1 is chloro.
The activated carbonate derivative A7 is coupled with the free amine A5 or any acceptable ammonium salt of the same by applying any appropriate Reagent Suitable for Carbamate Coupling, which may be selected from the following: a trialkylamine (such as NMe3, NEt3, diisopropylethylamine (DIPEA), isopropyldiethylamine, triisopropylamine), pyridine, or 4-(N,N-dimethylamino)pyridine (DMAP); preferably, NEt3 or DIPEA.
A9 is used as a precursor to introduce an appropriate self-immolative spacer for the release of phenolic cargos. The attachment point at the aromatic system can either be in para (1,4) or ortho (1,2) position relative to the phenolic alcohol. A9 can be prepared from e.g. commercially available 2-hydroxybenzyl alcohol or 4-hydroxybenzyl alcohol using literature known methods.
PG3 represents any protecting group for the benzylic alcohol, which intermediately masks the oxygen's nucleophilic reactivity and can be cleaved without affecting other functional groups in the molecule. Preferably PG3 may be one of the following O-protecting groups: methoxymethyl ether (MOM), tetrahydropyranyl ether (THP), tert-butyl ether (tBu), allyl ether, benzyl ether (Bn), tert-butyldimethylsilyl ether (TBS), tert-butyldiphenylsilyl ether (TBDPS), benzoate (Bz), acetate (Ac), pivalate (Piv). More preferably, PG3 can be selected from any of the following O-protecting groups: MOM, THP, tBu, TBS.
Transformation of the phenolic alcohol function of A9 into an activated carbonate derivative A10 may be achieved by any appropriate method for the transformation of alcohols into activated carbonate derivatives, as outlined above. LG4 depends on the selected method, as outlined above.
The activated carbonate A10 is coupled with the free amine A5 or any acceptable ammonium salt of the same by applying any reagent suitable for carbamate coupling, as outlined above, yielding A11.
Deprotection of A11 can generally be achieved by applying any appropriate deprotection conditions to give A12, which depending on PG3 may be selected from the General Deprotection Conditions as outlined above or from organic hydrogen fluoride solutions or any other hydrogen fluoride or fluoride source (HF/pyridine, alkali fluorides, alkaline earth fluorides, tetraalkylammonium fluorides). Preferred conditions are: organic hydrogen fluoride solutions or any other hydrogen fluoride or fluoride source (HF/pyridine, alkali fluoride, alkaline earth fluoride, tetraalkylammonium fluoride such as TBAF). More preferred are HF/pyridine or TBAF.
Activation of the benzylic alcohol A12 for nucleophilic substitution may be achieved by any activation method for alcohols. Preferably, this may be achieved using an activation reagent selected from the following list of Reagents for the Activation of Benzylic Alcohols: para-toluenesulfonyl chloride (TsCl), methanesulfonyl chloride (MsCl), trifluoromethanesulfonic anhydride (Tf2O), tetrachloromethane or tetrabromomethane or iodine activated with a trialkylphosphine or a triarylphosphine (e.g. PPh3/CBr4), thionyl chloride (SOCl2), phosphoryl chloride (POCl3), hydrochloric acid (HCl), or hydrobromic acid (HBr). Preferred are p-toluenesulfonyl chloride (TsCl), methanesulfonyl chloride (MsCl), thionyl chloride (SOCl2), phosphoryl chloride (POCl3), hydrochloric acid (HCl) or hydrobromic acid (HBr). Most preferred are methanesulfonyl chloride (MsCl) or hydrobromic acid (HBr).
Depending on the selected conditions for the activation of the benzylic alcohol, LG2 in A13 is any acceptable leaving group suitable for nucleophilic substitution, such as —Cl, —Br, —I, para-toluenesulfonate (OTs), methylsulfonate (OMs), or trifluoromethylsulfonate (OTf). Preferably, LG2 is —Br, —Cl, or OMs. More preferably, LG2 is —Br.
A1 is selected, so that A6 is a therapeutic, diagnostic or theranostic cargo of which the —OH group in A1-OH is a phenolic or aromatic hydroxyl group. A2 and A3 are selected, so that A14 is a therapeutic, diagnostic or theranostic cargo of which the —NH— group in A2-NH-A3 is an amine or aniline group.
The reaction of A13 with alcohol A6 yielding benzylic ethers A15a,b, or the reaction of A13 with amine A14 yielding benzylic amines A15c,d, can be achieved by any appropriate method for nucleophilic substitution, which includes the use of appropriate reagents for nucleophilic substitution, which may be selected from the following: (1) alkali or alkaline earth hydrides (e.g. NaH, CaH2), amides (e.g. NaNH2, LDA, LHMDS, KHMDS), alkoxides (e.g. KOtBu, NaOMe), hydroxides (e.g. NaOH, KOH), or carbonates (e.g. K2CO3, Cs2CO3); (2) trialkylamines (e.g. NMe3, NEt3, DIPEA, isopropyldiethylamine, triisopropylamine) or 4-dimethylaminopyridine (DMAP). Preferred are hydrides (NaH, CaH2), hydroxides (NaOH, KOH), or carbonates (K2CO3, Cs2CO3). More preferred are NaH, KOH or K2CO3.
Alternatively, preparation of benzylic ethers A15a,b can be achieved from reaction of A6 with A12 using any appropriate conditions effecting in situ activation of the benzylic alcohol, including using dialkyl azodicarboxylate activated with a trialkylphosphine or triarylphosphine (Mitsunobu conditions: e.g. PPh3/DIAD).
A12 is also used to introduce a benzyloxycarbonyl self-immolative spacer for the release of amine/aniline cargos. Transformation of the benzylic alcohol function of A12 into the activated carbonate A16 may be achieved by any of the methods for the transformation of alcohols into activated carbonate derivatives outlined above. LG1 depends on the selected method, as outlined above.
The activated carbonate A16 is coupled with aromatic alcohol A6 to yield A17a,b, or is coupled with amine A14 or any acceptable ammonium salt of the same to yield A17c,d, by applying any Reagent Suitable for Carbamate Coupling as outlined above.
Pharmaceutical or Diagnostic Compositions
The compounds of formula (I) can be administered to a patient in the form of a pharmaceutical or diagnostic composition which can optionally comprise one or more pharmaceutical carrier(s) or excipients.
The compounds of formula (I) can be administered by various well known routes, including oral, rectal and parenteral administration, e.g. intravenous, intramuscular, intradermal, subcutaneous, topical, and similar administration routes. Parenteral, oral and topical administration are preferred, particularly preferred is intravenous administration.
Particular preferred pharmaceutical or diagnostic forms for the administration of a compound of formula (I) are forms suitable for injectable use and include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. In all cases the final solution or dispersion form must be sterile and fluid. Typically, such a solution or dispersion will include a solvent or dispersion medium, containing, for example, water buffered aqueous solutions, e.g. biocompatible buffers, ethanol, polyol, such as glycerol, propylene glycol, polyethylene glycol, suitable mixtures thereof, surfactants or vegetable oils. A compound of the invention can also be formulated into liposomes, in particular for parenteral administration. Liposomes provide the advantage of increased half life in the circulation, if compared to the free drug and a prolonged more even release of the enclosed drug.
Sterilization of infusion or injection solutions can be accomplished by any number of art recognized techniques including but not limited to addition of preservatives like anti-bacterial or anti-fungal agents, e.g. parabene, chlorobutanol, phenol, sorbic acid or thimersal. Further, isotonic agents, such as sugars or salts, in particular sodium chloride may be incorporated in infusion or injection solutions.
Production of sterile injectable solutions containing one or several of the compounds of the invention is accomplished by incorporating the respective compound in the required amount in the appropriate solvent with various ingredients enumerated above as required followed by sterilization. To obtain a sterile powder the above solutions are vacuum dried or freeze dried as necessary. Preferred diluents of the present invention are water, physiologically acceptable buffers, physiologically acceptable buffer salt solutions or salt solutions. Preferred carriers are cocoa butter and vitebesole. Excipients which can be used with the various pharmaceutical or diagnostic forms of a compound of the invention can be chosen from the following non limiting list:
In one embodiment the formulation is for oral administration and the formulation comprises one or more or all of the following ingredients: pregelatinized starch, talc, povidone K 30, croscarmellose sodium, sodium stearyl fumarate, gelatin, titanium dioxide, sorbitol, monosodium citrate, xanthan gum, titanium dioxide, flavoring, sodium benzoate and saccharin sodium.
Other suitable excipients can be found in the Handbook of Pharmaceutical Excipients, published by the American Pharmaceutical Association, which is herein incorporated by reference.
The pharmaceutical or diagnostic compositions comprising a compound of the invention can be produced in a manner known per se to the skilled person as described, for example, in Remington's Pharmaceutical Sciences, 15th Ed., Mack Publishing Co., New Jersey (1991).
It is to be understood that depending on the severity of the disorder and the particular type which is treatable with one of the compounds of the invention, as well as on the respective patient to be treated, e.g. the general health status of the patient, etc., different doses of the respective compound are required to elicit a therapeutic or prophylactic effect.
The cargo is, as defined above, any therapeutic, diagnostic or theranostic agents. Depending on the therapeutic, diagnostic or theranostic agent, the mode of administration, amongst other factors, the appropriate dose spans a wide range. The determination of the appropriate dose lies within the discretion of the attending physician.
If desired, the pharmaceutical or diagnostic composition can comprise a second pharmaceutically active agent. The second pharmaceutically active agent is not limited and can be selected from a vascular disrupting agent, a cytotoxic chemotherapeutic agent and an immunomodulator. Examples thereof include combretastatin A-4 (CA4), 3′-aminocombretastatin A-4, BNC105, ABT-751, ZD6126, combretastatin A-1, or prodrugs of the same (which includes but is not limited to combretastatin A-4 phosphate (CA4P), 3′-aminocombretastatin A-4 3-serinamide (ombrabulin), combretastatin A-1 bisphosphate (CA1P), and BNC105 phosphate (BNC105P)), and pharmaceutically acceptable salts, solvates or esters of the same.
The mechanism by which reductive cleavage of the disulfide leads to release of the cargo agent, is shown in the following schemes depicting the release of H—O-A1 (phenol-type) or H—N(A2)(A3) (amine-type) cargos from compounds of the invention. Without wishing to be bound by theory, it is assumed that the reduction of the disulfide bond of the compounds of the invention could, in cells, occur by a 2-step transthiolation/thiol-resolution process performed by a reducing enzyme or protein with a dithiol or selenothiol active site, for example, a disulfide reductase or its effector disulfide protein such as thioredoxin, thioredoxin reductase, or glutathione reductase.
When L is a bond, the phenolic cargo (A1)-OH is directly linked to A as a carbamate, giving compounds of type A8. Upon reduction of the disulfide bond in A8, the reduced thiol/thiolate species A22 is formed. Depending on the redox microenvironment and local pH A22 can intramolecularly cyclise via an addition/elimination mechanism, which irreversibly releases the free phenolic cargo (i.e. therapeutic, diagnostic or theranostic agent) H—O-A1 (A6) as well as the byproduct carbamothioate species A23.
General Mechanism: When L is a benzylic (1,4)- or (1,6)-self-immolative linker which is linked to A as a carbamate, and is connected to B as a benzylic ether or benzylamine, then B can be selected from phenol-type (A1)-OH or amine-type HN(A2)(A3) cargos, giving compounds A15(a-d) as defined below. Upon reduction of the disulfide bond in A15(a-d), the corresponding reduced thiol/thiolate species A24(a-d) is formed. Depending on the redox microenvironment and local pH A24(a-d) can intramolecularly cyclise via an addition/elimination mechanism, which irreversibly releases the corresponding intermediate phenolic species A25(a-d) as well as carbamothioate byproduct A23. The nature of compounds A15(a-d) and how the corresponding intermediate A25(a-d) releases the cargo B are shown below:
When L is the (1,6)-self-immolative linker
and B is the phenolic cargo
In compounds of type A15a, L is a benzylic (1,6)-self-immolative linker and B is a phenolic (A1)-OH cargo which is connected to the linker as a benzylic ether. Reduction of 15a gives 24a which after cyclisation gives intermediate A25a. A25a can perform an intramolecular 1,6-elimination releasing the quinone-methide species A26 and the free phenolic cargo A6.
When L is the (1,4)-self-immolative linker
and B is the phenolic cargo
In compounds of type A15b, L is a benzylic (1,4)-self-immolative linker and B is a phenolic (A1)-OH cargo, which is connected to the linker as a benzylic ether. Reduction of 15b gives 24b which after cyclisation gives intermediate A25b. A25b can perform an intramolecular 1,4-elimination releasing the quinone-methide species A27 and the free phenolic cargo A6.
When L is the (1,6)-self-immolative linker
and B is the amine-type cargo
In compounds of type A15c, L is a benzylic (1,6)-self-immolative linker and B is an amine-type HN(A2)(A3) cargo, which is connected to the linker as a benzylamine. Reduction of 15c gives 24c which after cyclisation gives intermediate A25c. A25c can follow an intramolecular 1,6-elimination releasing the quinone-methide species A26 and the free amine-type cargo (i.e., therapeutic, diagnostic or theranostic agent) A14.
When L is the (1,4)-self-immolative linker
and B is the amine-type cargo
In compounds of type A15d, L is a benzylic (1,4)-self-immolative linker and B is an amine-type HN(A2)(A3) cargo, which is connected to the linker as a benzylamine. Reduction of 15d gives 24d which after cyclisation gives intermediate A25d. A25d can perform an intramolecular 1,6-elimination releasing the quinone-methide species A27 and the free amine-type cargo A14.
General Mechanism: When L is a benzylic (1,4)- or (1,6)-self-immolative linker which is linked to A as a carbamate, and is connected to B as a benzylic carbonate or carbamate, then B can be selected from phenol-type (A1)-OH or amine-type HN(A2)(A3) cargos, giving compounds A21(a-d) as defined below. Upon reduction of the disulfide bond in A21(a-d), the corresponding reduced thiol/thiolate species A28(a-d) is formed. Depending on the redox microenvironment and local pH A28(a-d) can intramolecularly cyclise via an addition/elimination mechanism, which irreversibly releases the corresponding intermediate phenolic species A29(a-d) as well as carbamothioate byproduct A23. The nature of compounds A21(a-d) and how the corresponding intermediate A29(a-d) releases the cargo B are shown below:
When L is the (1,6)-self-immolative linker
and B is the phenolic cargo
In compounds of type A21a, L is a benzylic (1,6)-self-immolative linker and B is a phenolic (A1)-OH cargo which is connected to the linker as a benzylic carbonate. Reduction of 21a gives 28a which after cyclisation gives intermediate A29a. A29a can perform an intramolecular 1,6-elimination releasing the quinone-methide species A26 as well as the carbonic acid species A30 which further eliminates carbon dioxide to give the free phenolic cargo A6.
When L is the (1,4)-self-immolative linker
and B is the phenolic cargo
In compounds of type A21b, L is a benzylic (1,4)-self-immolative linker and B is a phenolic (A1)-OH cargo which is connected to the linker as a benzylic carbonate. Reduction of 21b gives 28b which after cyclisation gives intermediate A29b. A29b can perform an intramolecular 1,4-elimination releasing the quinone-methide species A27 as well as the carbonic acid species A30 which further eliminates carbon dioxide to give the free phenolic cargo A6.
When L is the (1,6)-self-immolative linker
and B is the amine-type cargo
In compounds of type A21c, L is a benzylic (1,6)-self-immolative linker and B is an amine-type cargo HN(A2)(A3) which is connected to the linker as a benzylic carbamate. Reduction of 21c gives 28c which after cyclisation gives intermediate A29c. A29c can perform an intramolecular 1,6-elimination releasing the quinone-methide species A26 as well as the carbamic acid species A31 which further eliminates carbon dioxide to give the free phenolic cargo A14.
When L is the (1,4)-self-immolative linker
and B is the amine-type cargo
In compounds of type A21d, L is a benzylic (1,4)-self-immolative linker and B is an amine-type cargo HN(A2)(A3) which is connected to the linker as a benzylic carbamate. Reduction of 21d gives 28d which after cyclisation gives intermediate A29d. A29d can perform an intramolecular 1,4-elimination releasing the quinone-methide species A27 as well as the carbamic acid species A31 which further eliminates carbon dioxide to give the free phenolic cargo A14. Without wishing to be bound by theory, it is assumed that the specific bicyclic structure common to the presently claimed compounds can ensure that the disulfide bond is stabilized against nonspecific reduction.
It is furthermore assumed that in some of the claimed compounds this stabilised disulfide is reductively cleaved selectively by certain reductants, such as one or more oxidoreductases or redox effector proteins, and therefore that cargo release can be used as a quantification method for the activity of those reductants, which is a goal of probe development in diagnostics and in research.
It is furthermore assumed that in some of the claimed compounds this stabilised disulfide is reductively cleaved selectively in cells with dysregulated redox properties (including cells located in hypoxic environments, and cells with upregulated oxidoreductases) such as tumor cells and cells located in inflammatory microenvironments. Therefore, the effect of the agent that is released after reductive cleavage can be localized to pathological cells and environments which ensures that healthy cells and tissues are not exposed to similar levels of the agent. This pathology-specificity allows the presently claimed compounds which include a therapeutic or theranostic agent to be used as therapeutics for pathological cells and environments; and allows the presently claimed compounds which include a diagnostic or theranostic agent to be used as diagnostics for pathological cells and environments. Such selectivity is a longstanding goal of prodrug and diagnostic development, and offers a significant advantage compared to prior art disulfide-reduction-triggered compound designs which are activated by typical cellular conditions without specificity for pathological cells (see e.g. Hyman, L. M. et al. Coordination Chemistry Reviews 2012, 256, 2333-2356. https://doi.org/10.1016/j.ccr.2012.03.009).
The compounds having the formula (I) are particularly useful as therapeutics or diagnostics.
The compounds having the formula (I) can be used in the diagnosis, treatment, amelioration or prevention of a disorder selected from a neoplastic disorder; atherosclerosis; an autoimmune disorder; an inflammatory disease; a chronic inflammatory autoimmune disease; ischaemia; and reperfusion injury.
Preferably, the disorder is a neoplastic disorder, in particular cancer. The cancer is not particularly limited, preferably the cancer is selected from acoustic neuroma, adenocarcinoma, angiosarcoma, basal cell carcinoma, bile duct carcinoma, bladder carcinoma, breast cancer, bronchogenic carcinoma, cervical cancer, chondrosarcoma, chordoma, choriocarcinoma, craniopharyngioma, cystadenocarcinoma, embryonal carcinoma, endotheliosarcoma, ependymoma, epithelial carcinoma, Ewing's tumor, fibrosarcoma, hemangioblastoma, leiomyosarcoma, liposarcoma, Merkel cell carcinoma, melanoma, mesothelioma, myelodysplastic syndrome, myxosarcoma, oligodendroglioma, osteogenic sarcoma, ovarian cancer, pancreatic cancer, papillary adenocarcinomas, papillary carcinoma, pinealoma, prostate cancer, renal cell carcinoma, retinoblastoma, rhabdomyosarcoma, sebaceous gland carcinoma, seminoma, squamous cell carcinoma, sweat gland carcinoma, synovioma, testicular tumor, Wilms' tumor, adrenocortical carcinoma, urothelial carcinoma, gallbladder cancer, parathyroid cancer, Kaposi sarcoma, colon carcinoma, gastrointestinal stromal tumor, anal cancer, rectal cancer, small intestine cancer, brain tumor, glioma, glioblastoma, astrocytoma, neuroblastoma, medullary carcinoma, medulloblastoma, meningioma, leukemias including acute myeloid leukemia, multiple myeloma, acute lymphoblastic leukemia, liver cancer including hepatoma and hepatocellular carcinoma, lung carcinoma, non-small-cell lung cancer, small cell lung carcinoma, lymphangioendotheliosarcoma, lymphangiosarcoma, primary CNS lymphoma, non-Hodgkin lymphoma, and classical Hodgkin's lymphoma.
The therapeutic, diagnostic or theranostic compounds having the formula (I) can be used in combination with one or more other therapeutics, diagnostics or theranostics. The type of the other therapeutics, diagnostics or theranostics is not particularly limited and will depend on the disorder to be treated and/or diagnosed. Preferably, other therapeutics can enhance the selectivity and/or the turnover of the compound of formula (I) in the targeted tissue type; for example, the other therapeutic may be an agent that can stimulate transient hypoxia in tumors and thereby enhance the tumor-specific release of the cargo of the compound of formula (I) (which may be a therapeutic, diagnostic, or theranostic). Preferably in the case of compounds of formula (I) which are therapeutics and theranostics releasing a cargo used to treat a disorder, the other medicaments will be further therapeutics which are used to treat the same disorder; for example, further therapeutics that can treat a neoplastic disorder such as cancer may be applied in combination with compounds of formula (I) that are therapeutic proagents releasing a cargo that can treat a neoplastic disorder such as cancer.
The one or more other medicament, diagnostic or theranostic can be administered prior to, after, or simultaneously with the compound having formula (I). In another preferred embodiment, the other medicament can be an agent that can stimulate transient hypoxia in tumors which can be administered prior to, after or simultaneously to administration of compound of formula (I).
Agents which are capable of stimulating hypoxia in diseased tissues include but are not limited to vascular disrupting agents (including colchicine domain tubulin inhibitors such as combretastatin A-4 (CA4), 3′-aminocombretastatin A-4, BNC105, ABT-751, ZD6126, combretastatin A-1, or prodrugs of the same (which includes but is not limited to combretastatin A-4 phosphate (CA4P), 3-aminocombretastatin A-4 3′-serinamide (ombrabulin), combretastatin A-1 bisphosphate (CA1P), and BNC105 phosphate (BNC105P), and pharmaceutically acceptable salts of the same), vascular-acting drugs such as vasodilator or antihypertensive drugs (including hydralazine), and inhibitors of prolyl hydroxylase or stabilizers of hypoxia-inducible factor 1 such as daprodustat, desidustat and molidustat.
For in vivo usage, a diagnostic compound of the invention is administered to a subject or patient by any acceptable route, preferably i.v., i.p. or per os, and the subject or patient is imaged thereafter according to the imaging modality of the cargo, preferably by fluorescence imaging, photoacoustic imaging, luminescence imaging, or positron emission tomography. For usage in vitro, a diagnostic compound of the invention is administered to a test sample which, for instance, has been obtained from a subject, and the test sample is imaged thereafter according to the imaging modality of the cargo, preferably by fluorescence imaging, absorbance, or luminescence imaging; the sample may preferably be cells, a tissue section, a small animal model or a biopsy sample.
The compounds of the present invention can be used to predict the suitability of a compound of the invention for treating a patient who is suffering from a disorder selected from a neoplastic disorder; atherosclerosis; an autoimmune disorder; an inflammatory disease; a chronic inflammatory autoimmune disease; ischaemia; and reperfusion injury.
In a first step, a sample of the effected tissue or body fluid is obtained from the patient. The sample is then contacted with a compound (I*) of the present invention, wherein A is A*, L is L* and A1 is selected such that A1-OH is a diagnostic or theranostic agent or A2 and A3 are independently selected such that A2-NH-A3 is a diagnostic or theranostic agent. Subsequently, the sample is imaged according to the imaging modality of the diagnostic or theranostic agent, for instance, by fluorescence imaging, photoacoustic imaging, luminescence imaging, or positron emission tomography, to determine whether the compound (I*) of the present invention has been reductively cleaved to release A1-OH or A2-NH-A3. If compound of the present invention has been reductively cleaved then it can be assumed that a compound (I**) of the present invention which has the same moieties A* and L* as the compound (I*) but in which A1-OH is a therapeutic or theranostic agent or A2 and A3 are independently selected such that A2-NH-A3 is a therapeutic or theranostic agent will be effective for treating the patient. The theranostic agent which is used in the compounds (I*) and (II*) can be the same or different.
The extent to which the compound (I*) of the present invention is reductively cleaved to release A1-OH or A2-NH-A3 is not particularly limited. It should be sufficient to provide a therapeutically effective amount of A1-OH or A2-NH-A3 to the tissue or body fluid. For instance, 0.1-20%, preferably 0.5-5% of the compound (1*) of the present invention can be reductively cleaved.
In the following, examples of preferred compounds having the formula (I) are described, to illustrate some of the ways in which conjugation of a cargo H-B as the compound A-L-B masks its diagnostic and/or therapeutic activity, until the cargo is released following reduction. The further example compounds of the invention P1-P22 are described in greater detail below.
The examples 1-15 include the following therapeutic designs:
It is illustrated by 1, 4, 9, 11 and 14 that a broad range of bioactive phenolic cargos with a range of different bioactivity mechanisms and disease indication scopes can therefore be used, including with extensive structural substitutions, within the scope of the invention to deliver cargo-release-based activation of proagent bioactivity, i.e. functional prodrugs dependent upon triggering of the key disulfide of a compound having the formula (I).
It is illustrated by 13 and 15 that a broad range of bioactive amine cargos with different bioactivity mechanisms and disease indication scopes can therefore be used in conjunction with a range of spacers, each with optional extensive structural substitutions, while remaining within the scope of the invention to deliver cargo-release-based activation of proagent bioactivity, i.e. functional prodrugs dependent upon triggering of the key disulfide of a compound having the formula (I).
The examples 1-15 include the following diagnostic designs:
It is illustrated by 2, 3, 5, 7, 8, 10 and 12 that a broad range of diagnostically active phenol or amine cargos, enabling a range of structures and diagnostic detection methods, can therefore be used, including with extensive structural substitutions, within the scope of the invention, to deliver cargo-release-based activation of proagent diagnostic activity, i.e. functional diagnostic probes dependent upon triggering of the key disulfide of a compound having the formula (I).
The examples 1-15 also include the theranostic design 6 that releases a cargo subunit that is leuco-methylene blue, a reduced form of the theranostic compound methylene blue which after release in biological media is spontaneously converted to methylene blue (similar principles are known for a range of related prodrugs). Methylene blue can be useful therapeutically including as a photosensitizer in photodynamic therapy (e.g. of the autoimmune disorder psoriasis, or of cancers) and/or as a redox-active selective toxin (e.g. against malaria parasite); as well as diagnostically (e.g. as a fluorescent agent or a photoacoustic dye); therefore being capable of theranostic activity (Schirmer, R. H. et al. Neurobiology of Aging 2011, 32, 2325.e7-2325.e16. https://doi.org/10.1016/j.neurobiolaging.2010.12.012). Note that a range of functionally and structurally related compounds (e.g. phenoxazines) can also be used as cargo subunits in theranostics following the same design principles while remaining within the scope of a compound having the formula (I).
Various modifications and variations of the invention will be apparent to those skilled in the art without departing from the scope of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the described modes for carrying out the invention which are obvious to those skilled in the relevant fields are intended to be covered by the present invention.
The invention will be more fully understood by reference to the following examples. They should not, however, be construed as limiting the scope of the invention.
Unless stated otherwise, (1) all reactions and characterisations were performed with solvents and reagents, used as obtained, under closed air atmosphere without special precautions; (2) DCM, Et2O, THF and DMF used for synthesis were distilled, then dried on activated molecular sieves; (3) isohexane and EtOAc used for chromatography was distilled from commercial crude iso-hexane fraction; (4) “column” and “chromatography” refer to FCC, performed on Merck silica gel Si-60 (40-63 μm); (5) procedures and yields are unoptimised; (6) yields refer to isolated chromatographically and spectroscopically pure materials, corrected for residual solvent content.
NMR: Standard NMR characterisation was carried out by 1H- and 13C-NMR spectra. Avance Ill Bruker BioSpin 400 MHz, 500 MHz and 600 MHz spectrometers were used (1H: 400 MHz, 500 MHz or 600 MHz, 13C: 101 MHz, 126 MHz and 151 MHz respectively) typically at 298 K, although with some carbamates spectroscopy was simplified by measuring at 373 K to induce a dynamic equilibrium between their rotameric species. Chemical shifts (6) are reported in ppm calibrated to residual non-perdeuterated solvent as an internal reference. Peak descriptions singlet (s), doublet (d), triplet (t), quartet (q), pentuplet (p), and multiplet (m) are used. Coupling constants J are given in Hz.
Mass spectra: Unit mass measurements were performed on an AGILENT 1200 SL coupled LC-MS system with ESI mode ionisation, with binary eluent mixtures of H2O:MeCN, with the water containing formic acid. HRMS was carried out by the Zentrale Analytik of the Ludwig-Maximilian-University (LMU), Munich (electron impact (EI) at 70 eV with a Thermo Finnigan MAT 95 or a Jeol GCmate 11 spectrometer; electrospray ionization (ESI) with a Thermo Finnigan LTQ FT Ultra Fourier Transform Ion Cyclotron resonance mass spectrometer) in positive or negative mode as stated.
To a solution of the amine (1.0 eq) in dioxane:H2O (1:1; 0.3 M) was added NEt3 (1.3 eq per amine function). A solution of Boc2O (1.2 eq per amine function) in dioxane was added to the reaction mixture and the resulting biphasic solution was stirred at 25° C. for 15 h. The reaction mixture was then acidified to pH<4 using aq. HCl (2 M), EtOAc was added, and the organic phase was separated and washed with a sat. aq. NaCl. The combined aq. layers were extracted with EtOAc (3×), then the combined organic layers were dried over Na2SO4, filtered and concentrated to afford the desired N-Boc-protected amine as a colourless solid. Further purification was achieved by FCC or by recrystallization from EtOH or MeOH.
Step 1: To a solution of the primary or secondary alcohol in anhydrous pyridine (0.3 M) at 0° C. was added methane sulfonyl chloride (1.1 eq per alcohol function) and the mixture was stirred at 0° C. for 30 min, was then allowed to warm to r.t. and was further stirred for 2-15 h. The resulting mixture was poured into ice water (water:pyridine, 5:1) and precipitation of a colourless solid was observed. The solid was filtered off, washed with cold water and cold diethylether and then dried to obtain the crude material as a colourless solid.
Step 2: The material obtained in step 1 was dissolved in acetone (0.1 M) or anhydrous DMF (0.05 M), solid KSAc (1.1 eq per alcohol function) was added and the resulting mixture was stirred at reflux for 4-15 h, before being cooled to r.t. and concentrated under reduced pressure. The residue was taken into EtOAc and washed with aq. NaCl (3×). The combined aq. layers were extracted with EtOAc (3×), then the combined organic layers were dried over Na2SO4, filtered and concentrated. Further purification was achieved by FCC (isohexane/EtOAc) to afford the desired bis(ethanethioate) derivatives.
Similarly to Raines, Lukesh et al. (Lukesh, J. C. et al. J. Am. Chem. Soc. 2012, 134, 4057-4059. https://doi.org/10.1021/ja211931f) an oven-dried round-bottom flask was charged with a bis(ethanethioate) derivative (1 eq) and KOH (0.17 M in MeOH). The solution was stirred under air for 16 h. Reaction progress was monitored by LC-MS. In case of incomplete oxidation to the disulfide, a methanolic solution of I2 was added dropwise until a permanently coloured solution was observed. The solvent was removed under reduced pressure, the resulting oil was taken into aq. Na2S2O3 (which immediately removed any remaining colouration) and the mixture was extracted with EtOAc (4×). The combined organic layers were dried over Na2SO4, filtered and concentrated under reduced pressure to afford the desired product as a colourless solid. No further purification was required.
A N-Boc protected primary or secondary amine was dissolved in anhydrous DCM (0.1 M) and a solution of HCl (10 eq per amine function) in dioxane (4 M) was added dropwise. The mixture was stirred at r.t. for 2-15 h and was then added dropwise to cold Et2O (Et2O:DCM/dioxane, 10:1) and precipitation of a colourless solid was observed. The solid was filtered off, washed with Et2O and dried under reduced pressure to obtain the desired primary or secondary amine hydrochloride salt as a colourless solid. No further purification was required.
Step 1: Similarly to Felber et al. (Felber, J. et al. ChemRxiv 2020. https://doi.org/10.26434/chemrxiv.13483155.v1) under inert gas atmosphere the phenolic compound (1 eq) was dissolved in anhydrous DCM (0.02 M). The reaction mixture was cooled to 0° C. and a solution of triphosgene (BTC) (1.05 eq, 0.5 M in dry DCM) was added dropwise, followed by the addition of DIPEA (1.0 eq) dissolved in anhydrous DCM (1 M). The mixture was stirred at 0° C. for 0.5 h, was warmed to r.t. and further stirred for 0.5 h. All volatiles were removed under reduced pressure employing an external liquid nitrogen trap to afford the corresponding phenolic chloroformate as a solid.
Step 2: The secondary amine hydrochloride (0.5-1.1 eq) was suspended in anhydrous DCM (0.05 M), and DIPEA (1.1 eq) was added and a clear solution was observed, which was added dropwise to a solution of the phenolic chloroformate in anhydrous DCM (0.02 M) was added dropwise at 0° C. and the mixture was stirred for 0.5 h, was allowed to warm to r.t. and was further stirred for 0.5 h. A sat. aq. solution of NaHCO3 was added and the mixture was diluted with DCM. The organic layer was separated and washed with sat. aq. NaCl. The combined aq. layers were extracted with DCM (2×) and the combined organic layers were dried over Na2SO4, filtered and concentrated under reduced pressure, to afford the crude product as a coloured solid. Purification using FCC gave the desired product as a colourless solid.
Step 1: To a solution of commercial dimethyl pyridine-2,3-dicarboxylate X1 (8.00 g, 41.0 mmol) in MeOH (35 mL) was added 96% H2SO4 (0.22 mL, 4.1 mmol) and 10% Pd/C (250 mg, 0.235 mmol). The mixture was stirred under H2-atmosphere (40 bar) for 15 h, filtered over Kieselgur and concentrated to afford compound X2 (8.09 g, 40.2 mmol 98%) as a colourless oil. The material was used without further purification.
Step 2: Prepared from the material obtained in step 1 (3.00 g, 14.9 mmol) according to general protocol A. Compound X3 (4.43 g, 14.7 mmol, 99%, 97% over 2 steps) was obtained as a colourless solid by FCC (isohexane/EtOAc).
Rf=0.35 (isohexane:EtOAc, 2:1). HRMS (ESI+): C14H23NNaO6: calc. 324.14176. found 324.14165. 1H-NMR (400 MHz, tetrachlorethane-d2, 373 K): δ (ppm)=5.32 (s, 1H), 4.05-3.87 (m, 1H), 3.68 (s, 3H), 3.66 (s, 3H), 2.85-2.71 (m, 1H), 2.59 (ddd, J=12.7, 5.0, 3.6 Hz, 1H), 2.11-1.92 (m, 1H), 1.74-1.66 (m, 1H), 1.61 (td, J=13.3, 3.9 Hz, 1H), 1.45 (s, 9H), 1.44-1.36 (m, 1H). 13C-NMR (101 MHz, tetrachlorethane-d2, 373 K): δ (ppm)=171.7, 170.3, 155.0, 80.5, 55.9, 51.9, 51.7, 43.5, 41.3, 28.5, 24.3, 22.6.
Step 1: X2 was prepared from dimethyl pyridine-2,3-dicarboxylate (4.11 g, 21.1 mmol) as outlined above.
Step 2: The material obtained in step 1 was mixed with anhydrous THF (20 mL). The solution was added dropwise to a suspension of LiAlH4 (2.40 g, 63.3 mmol, 3.0 eq.) in anhydrous THE (125 mL) at 0° C. under N2-atmosphere. The mixture was allowed to warm to r.t. and was further stirred at r.t. for 15 h, was then cooled to 0° C. and EtOAc (5 mL), MeOH (5 mL), water (10 mL) and 2 M aq. NaOH (10 mL) were added carefully in the specified order and filtered. The solids were washed with THF (2×30 mL) and discarded. The filtrate was evaporated under reduced pressure. The residue as taken up in THF (30 mL) and MeOH (10 mL). After drying over Na2SO4 all volatiles were removed under reduced pressure and the residue containing X4 was directly used further.
Step 3: To residue obtained in step 2 were added EtOH (45 mL), K2CO3 (7.29 g, 52.8 mmol, 2.5 eq.) and PMB-CI (4.29 mL, 31.7 mmol, 1.5 eq.). The mixture was stirred at r.t. for 15 h, then the solids were filtered off, washed with EtOH and discarded. The filtrate was evaporated under reduced pressure, 2 M aq. HCl (60 mL) was added and the mixture was washed with DCM (3×30 mL). The organic washings were discarded, the aqueous solution was basified with 2 M aq. NaOH (70 mL) and extracted with DCM (2×60 mL). The extracts were dried over Na2SO4 and evaporated under reduced pressure. The residue was purified by FCC to afford compound X5 (2.36 g, 8.89 mmol, 42% over 3 steps) as a faint orange oil.
Rf=0.20 (EtOAc). HRMS (ESI+): C15H24NO3: calc. 266.17507. found 266.17468. 1H-NMR (400 MHz, CDCl3): δ (ppm)=7.24 (d, J=8.5 Hz, 2H), 6.86 (d, J=8.6 Hz, 2H), 3.86 (d, J=13.1 Hz, 1H), 3.81 (t, J=9.5 Hz, 1H), 3.79 (s, 3H), 3.71 (d, J=13.1 Hz, 1H), 3.67-3.52 (m, 3H), 2.98-2.90 (m, 1H), 2.74 (ddd, J=13.6, 10.6, 3.3 Hz, 1H), 2.49 (dd, J=13.5, 3.9 Hz, 1H), 2.17 (dd, J=10.5, 4.4 Hz, 1H), 1.74-1.60 (m, 2H), 1.54-1.37 (m, 2H). 3C-NMR (101 MHz, CDCl3): δ (ppm)=159.0, 130.8, 130.1, 114.0, 64.9, 60.8, 57.5, 57.3, 55.4, 46.4, 36.4, 25.0, 20.9.
Step 1: A mixture of compound X3 (5.33 g, 17.7 mmol, 1.0 eq.), anhydrous MeOH (50 mL) and NaOMe (1.15 g, 21.2 mmol, 1.2 eq.) was stirred under N2-atmosphere at 55° C. for 15 h, before AcOH (1.52 mL, 26.5 mmol, 1.5 eq.) was added and all volatiles were removed under reduced pressure. The residue was suspended in DCM and filtered. The filtrate was diluted with isohexane and directly purified by FCC (isohexane/EtOAc) to afford a cis/trans mixture of 1-(tert-butyl)dimethyl piperidine-1,2,3-tricarboxylate X6 (4.08 g, 13.5 mmol, 76%; 62% trans) as a yellow oil.
Step 2: To a suspension of LiAlH4 (1.54 g, 40.6 mmol, 3.0 eq.) in anhydrous THF (80 mL) at 0° C. under N2-atmosphere was dropwise added a solution of the material obtained in step 1 (4.08 g, 13.5 mmol, 1.0 eq.) in anhydrous THF (5.5 mL). The mixture was stirred for 30 min, was then allowed to warm to r.t. and further stirred for 2 h. The mixture was cooled to 0° C., then EtOAc (5 mL), MeOH (5 mL), water (10 mL) and 2 M aq. NaOH (10 mL) were added carefully in the specified order and filtered. The solids were washed with a small amount of THF and discarded. The filtrate was evaporated under reduced pressure, Et2O (50 mL) and Na2SO4 (30 g) were added and the mixture was filtered. The filtrate was evaporated under reduced pressure and remaining yellowish oil (3.16 g, 12.9 mmol, 95%) was further used in the next step. An analytically pure sample of compound X7 was obtained by FCC (isohexane/EtOAc).
Rf=0.30 (EtOAc). HRMS (EI) for C11H20NO3 (M-CH2O): calc. m/z 214.1438. found m/z 214.1435. 1H-NMR (400 MHz, CDCl3) δ (ppm)=4.41 (s, 1H), 3.86 (dd, J=11.1, 7.3 Hz, 1H), 3.69-3.52 (m, 3H), 3.08 (s, 2H), 2.79 (m, 1H), 2.73 (s, 1H), 1.93 (dq, J=7.9, 3.7 Hz, 1H), 1.90-1.80 (m, 1H), 1.69 (d, J=5.2 Hz, 1H), 1.63-1.53 (m, 1H), 1.45 (s, 9H), 1.43-1.38 (m, 2H). 13C-NMR (101 MHz, CDCl3) δ (ppm)=171.3, 80.1, 68.1, 64.6, 60.5, 59.6, 40.7, 28.5, 25.7, 25.3, 22.7, 21.2, 14.3.
PPh3 (6.28 g, 23.9 mmol) was dissolved in anhydrous THE (0.25 M) and DIAD (4.7 mL, 23.9 mmol) was added dropwise at 0° C. Compound X7 (2.35 g, 9.58 mmol) was dissolved in anhydrous THF (0.1 M) and pre-mixed with HSAc (1.7 mL, 23.9 mmol) before being added to the reaction mixture at 0° C. similar to Raines, Lukesh et al. (Lukesh, J. C. et al. J. Am. Chem. Soc. 2012, 134, 4057-4059. https://doi.org/10.1021/ja211931f). All volatiles were removed and the residue was suspended in a mixture of Et2O/isohexane (1/2, 150 mL) and filtered. The solids were washed with additional Et2O/isohexane (1/2, 60 mL) and discarded. The filtrate was evaporated under reduced pressure to give compound X8 (4.93 g) as crude material. An analytically pure sample was obtained separately by FCC.
Rf=0.50 (isohexane:EtOAc, 3:1). HRMS (ESI+): C16H27NNaO4S2: calc. 384.12737. found 384.12765. 1H-NMR (400 MHz, CDCl3): δ (ppm)=4.26 (s, 1H), 4.15-3.80 (m, 1H), 3.33-3.13 (m, 1H), 3.06 (s, 1H), 2.99 (dd, J=13.7, 6.4 Hz, 1H), 2.88 (dd, J=13.7, 8.4 Hz, 1H), 2.73 (s, 1H), 2.33 (s, 3H), 2.32 (s, 3H), 1.89-1.74 (m, 2H), 1.70-1.52 (m, 2H), 1.45 (s, 9H), 1.44-1.39 (m, 1H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=195.7, 195.3, 155.4, 80.0, 52.6, 37.7, 36.3, 31.4, 30.8, 30.7, 29.8, 28.5, 23.1, 19.9.
Prepared from the material containing compound X8 obtained in the previous step (4.93 g) according to a 2-step sequence, first by general protocol C with purification by FCC, followed by general protocol D, giving compound X9 (908 mg, 4.29 mmol, 45% over 3 steps) with stereochemical purity (pure trans, racemic).
1H-NMR (400 MHz, DMSO-d6): 9.34 (d, J=20.9 Hz, 2H), 3.29-3.16 (m, 2H), 3.11 (dd, J=13.0, 3.1 Hz, 1H), 3.00 (dd, J=12.9, 11.3 Hz, 1H), 2.97-2.84 (m, 2H), 2.82 (dd, J=13.8, 10.9 Hz, 1H), 2.01 (tdd, J=10.9, 7.4, 3.3 Hz, 1H), 1.82-1.69 (m, 2H), 1.64 (d, J=13.1 Hz, 1H), 1.34 (dt, J=21.0, 12.4 Hz, 1H). 13C-NMR (101 MHz, DMSO-d6): δ9.4, 43.9, 40.2, 38.6, 34.2, 28.8, 21.9.
Prepared from compound X3 (1.11 g, 4.18 mmol) according to general protocol B using KSAc (3.0 eq) in DMF (0.05 M), giving X10 (922 mg) after FCC (isohexane/EtOAc) as a colourless oil, which was used for for the next steps without further purification. An analytically pure sample was obtained separately by FCC (isohexane/EtOAc).
Rf=0.36 (isohexane:EtOAc, 4:1). HRMS (ESI): C19H28NO3S2: calc. 382.15051. found 382.15002. 1H-NMR (400 MHz, CDCl3): δ (ppm)=7.24 (d, J=8.4 Hz, 2H), 6.84 (d, J=8.6 Hz, 2H), 3.80 (s, 3H), 3.67 (s, 2H), 3.25 (dd, J=13.6, 8.0 Hz, 1H), 2.99 (dd, J=13.6, 5.4 Hz, 1H), 2.91 (dt, J=8.5, 4.5 Hz, 1H), 2.88-2.77 (m, 2H), 2.60 (td, J=12.3, 11.3, 3.2 Hz, 1H), 2.44 (d, J=13.3 Hz, 1H), 2.33 (s, 3H), 2.33 (s, 3H), 2.13-2.00 (m, 1H), 1.75-1.65 (m, 1H), 1.63-1.53 (m, 1H), 1.48-1.40 (m, 1H), 1.40-1.30 (m, 1H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=196.4, 195.8, 158.7, 131.8, 129.8, 113.7, 61.0, 57.8, 55.4, 45.3, 37.6, 32.0, 30.8, 30.7, 26.0, 25.5, 22.3.
Prepared according to general protocol C from the 922 mg of material containing X10 obtained in the previous step. Compound X11 (629 mg, 2.13 mmol, 51% over 3 steps) was obtained by FCC (EtOAc/isohexane) as a yellowish oil.
Rf=0.47 (isohexane:EtOAc, 4:1). HRMS (ESI): C15H22NOS2: calc. 296.11373. found 296.11341. 1H-NMR (400 MHz, DMSO-d6): δ (ppm)=7.22 (d, J=8.2 Hz, 2H), 6.87 (d, J=8.5 Hz, 2H), 3.73 (s, 3H), 3.66 (d, J=12.5 Hz, 1H), 3.52 (d, J=12.8 Hz, 1H), 3.40-3.31 (m, 1H), 3.25 (d, J=13.0 Hz, 1H), 2.94 (d, J=9.6 Hz, 1H), 2.84 (d, J=12.9 Hz, 1H), 2.70 (dd, J=12.3, 7.1 Hz, 1H), 2.49-2.32 (m, 2H), 2.25-2.14 (m, 1H), 2.09 (d, J=10.9 Hz, 1H), 1.69-1.56 (m, 1H), 1.56-1.48 (m, 1H), 1.34 (d, J=11.8 Hz, 1H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=158.2, 131.1, 129.5, 113.6, 58.2, 56.5, 55.0, 45.0, 41.8, 36.8, 25.0, 22.6.
1-chloroethyl chloroformate (362 μL, 3.34 mmol, 2.0 eq.) was added dropwise to a solution of compound X11 (494 mg, 1.67 mmol, 1.0 eq.) in anhydrous THF (5 mL) at 0° C. The mixture was warmed to r.t. and stirred for 3 days, before Et2O (30 mL) was added and the mixture was washed with 2 M aq. HCl (25 mL). The aq. washings were extracted with Et2O (2×30 mL), dried over MgSO4, filtered and concentrated under reduced pressure. The residue was purified by FCC (DCM/isohexane), collecting a fraction with Rf=0.5 (DCM). To the resulting colourless oil was added anhydrous MeOH (50 mL), the mixture was stirred at 80° C. for 1 h and evaporated under reduced pressure. The residue was washed with Et2O to obtain compound X12 (207 mg, 0.977 mmol, 58%) as an off-white solid.
1H-NMR (400 MHz, DMSO-d6, 373 K): δ (ppm)=9.55 (s, 2H), 3.66 (dt, J=6.7, 3.2 Hz, 1H), 3.51 (dd, J=13.9, 7.6 Hz, 1H), 3.29 (s, 1H), 3.14 (d, J=12.9 Hz, 2H), 3.04-2.85 (m, 1H), 2.35 (s, 1H), 1.91 (s, 1H), 1.82 (ddq, J=12.6, 8.4, 4.3 Hz, 1H), 1.76-1.65 (m, 1H), 1.61 (td, J=9.2, 4.5 Hz, 1H).
To a solution of commercial cis-1,4-dibenzyloxy-2-butene (15.8 g, 58.9 mmol) acetone:H2O (3:1, 260 mL) were added 4-methylmorpholine N-oxide (19.9 g, 147 mmol) and K2[OsO2(OH)4] (141 mg, 0.383 mmol). The reaction mixture was stirred at r.t. for 15 h, before being poured into sat. aq. sodium bisulfite solution. The aq. layer was extracted with EtOAc (3×200 mL) and the combined organic layers were washed with sat. aq. NaCl, dried and concentrated under reduced pressure to give cis-X25 as a colourless solid (16.5 g, 54.6 mmol, 93%) without further purification.
Rf=0.33 (hexanes:EtOAc, 1:1). HRMS (EI+): m/z calc. for C18H22O4: [M]+ 302.1518. found: 302.1523. 1H-NMR (400 MHz, CDCl3): δ (ppm)=7.41-7.27 (m, 9H), 4.55 (s, 4H), 3.84 (dq, J=4.2, 1.7 Hz, 2H), 3.76-3.56 (m, 4H), 2.68 (s, 2H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=137.89, 128.61, 127.99, 127.92, 73.66, 71.54, 71.19.
Step 1: m-CPBA (77%, 33.1 g, 148 mmol) was added in small portions to a solution of X24 (33.0 g, 123 mmol) in DCM (120 mL) at 0° C. The reaction mixture was allowed to warm to r.t. and further stirred for 15 h, before being poured into sat. aq. NaHCO3 (200 ml) and the aq. layer was extracted with DCM (3×150 mL). The combined organic layers were washed with sat. aq. NaCl and dried over MgSO4, filtered and concentrated under reduced pressure and purified by FCC (hexanes/EtOAc) to give cis-1,4-dibenzyloxy-2,3-epoxybutane as a colourless oil (28.1 g, 99 mmol, 80%).
Step 2: To a solution of combined fractions of cis-1,4-dibenzyloxy-2,3-epoxybutane (37.2 g, 131 mmol) in THF:water (6:1, 280 mL) H2SO4 (34.9 mL, 654 mmol, 5.0 eq.) was added at 0° C. The reaction mixture was allowed to warm to r. t. and was further stirred for 15 h, before being diluted with water (300 mL) and EtOAc (200 mL) and the aq. layer was extracted with EtOAc (3×150 mL). The combined organic layers were washed with sat. aq. NaCl and dried over MgSO4, filtered concentrated under reduced pressure and purified by FCC (isohexane/EtOAc 3:1) to give trans-X25 as a colourless oil (25.7 g, 85.0 mmol, 65%).
Rf=0.38 (isohexane:EtOAc, 1:1). HRMS (EI): for C18H23O4: calc. 303.1591. found: 303.1595. 1H-NMR (400 MHz, CDCl3): δ (ppm)=7.39-7.25 (m, 10H), 4.61-4.49 (m, 4H), 3.92-3.84 (m, 2H), 3.67-3.53 (m, 4H), 2.74 (s, 2H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=137.9, 128.6, 128.0, 127.9, 73.8, 72.1, 70.7.
Step 1: cis-X25 (16.5 g, 54.6 mmol) was dissolved in pyridine (600 mL, 0.1 M) and methane sulfonyl chloride (10.0 mL, 130.0 mmol) was added at 0° C. The reaction was stirred for 1 h, was then allowed to warm to r.t. and was further stirred for 15 h, before being poured into cold water (5.0 L). The solid precipitate was filtered off, was washed with H2O and dried under reduced pressure to give (cis)-1,4-bis(benzyloxy)butane-2,3-diyl dimethanesulfonate (22.1 g, 48.2 mmol, 88%) as a colourless solid.
Step 2: A mixture of the material obtained in step 1 and sodium azide (7.21 g, 111 mmol, 2.3 equiv.) in DMSO (140 mL, 0.3 M) was stirred at 80° C. for 96 h, cooled to r.t., and the colourless suspension was diluted with water/brine (1:1, 300 mL). The aq. layer was extracted with EtOAc (3×150 mL) and the combined organic layers were dried over Na2SO4, filtered and concentrated under reduced pressure. The yellow crude oil was purified by FCC (hexane/EtOAc 9:1) giving cis-X26 as a colourless liquid (15.8 g, 44.8 mmol, 93%, 81% over 2 steps).
Rf=0.45 (isohexane:EtOAc, 9:1). LRMS (ESI+): for C18H20N6O2: calc. 325.2. found: 325.1. 1H-NMR (400 MHz, CDCl3): δ (ppm)=7.44-7.27 (m, 10H), 4.58 (s, 4H), 3.80-3.71 (m, 2H), 3.67 (m, 4H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=137.6, 128.6, 128.0, 127.8, 73.7, 69.8, 61.1.
Step 1: To a solution of trans-X25 (6.6 g, 22 mmol, 1.0 eq.) and pyridine (8.8 mL, 0.11 mol, 5.0 eq.) in dry DCM (45 mL), methane sulfonyl chloride (3.7 mL, 48 mmol, 2.2 eq.) was added dropwise at 0° C. The reaction mixture was stirred for 1 h, was then allowed to warm to r.t. and further stirred for 15 h, before being diluted with DCM (50 mL), poured into sat. aq. NH4Cl (200 mL) and extracted with EtOAc (3×100 mL). The combined organic layers were washed with sat. aq. NaCl, dried over MgSO4 and concentrated under reduced pressure to yield (trans)-1,4-bis(benzyloxy)butane-2,3-diyl dimethanesulfonate as a colourless solid.
Step 2: A mixture of yield (trans)-1,4-bis(benzyloxy)butane-2,3-diyl dimethanesulfonate (2.3 g, 5.0 mmol), and sodium azide (0.82 g, 13 mmol) in DMSO (15 mL) was stirred at 80° C. for 36 h, then cooled to r.t. and poured into water/NaHCO3 (1:1, 300 mL) and extracted with EtOAc (3×100 mL). The combined organic layers were washed with sat. aq. NaCl, dried over MgSO4, concentrated under reduced pressure and purified by FCC (isohexane/EtOAc) to give the trans-X26 as a colourless oil (1.5 g, 4.2 mmol, 81% over 2 steps).
Rf=0.46 (isohexane:EtOAc, 9:1). 1H-NMR (400 MHz, CDCl3): δ (ppm)=7.39-7.28 (m, 10H), 4.55 (s, 4H), 3.79-3.71 (m, 2H), 3.69-3.64 (m, 4H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=137.5, 128.7, 128.1, 127.9, 73.7, 69.8, 61.1.
Step 1: To a solution of cis-X26 (15.7 g, 44.6 mmol) in MeOH (400 mL, 0.1 M) was added Pd/C (10%, 634 mg). The mixture was stirred at r.t. under H2-atmosphere at 1 bar for 15 h. The catalyst was removed by filtration over Kieselgur and solvent was removed under reduced pressure to give (cis)-1,4-bis(benzyloxy)butane-2,3-diamine as a colourless oil (12.6 g, 41.9 mmol, 94%).
Step 2: The material obtained in step 1 was charged with concentrated HCl (186 mL, 2.2 mol, 50 equiv.) and was heated to 80° C. for 8 h, was then further stirred at r.t. for 15 h. The reaction mixture was concentrated under reduced pressure and the residue was dried by azeotropic evaporation using toluene (2×200 mL), was then washed with Et2O (300 mL) and dried under reduced pressure to give the 1,4-dihydroxybutane-2,3-diamine dihydrochloride as a brown solid without further purification.
Step 3: Following general protocol A, the material obtained in step 2 was reacted with Boc2O (23.4 g, 107 mmol) and NEt3 (50 mL, 357 mmol) to give cis-X27 as a colourless solid (11.1 mg, 34.7 mmol, 78% over 3 steps).
Rf=0.32 (hexanes/EtOAc 1:1). HRMS (EI+): m/z calc. for C18H22O4: [M]+ 320.1947. found: 302.1946. 1H-NMR (400 MHz, DMSO-d6): δ (ppm)=6.42 (d, J=7.3 Hz, 2H), 4.49 (t, J=5.6 Hz, 2H), 3.51 (q, J=5.3, 4.8 Hz, 2H), 3.41 (t, J=4.6, 4.2 Hz, 4H), 1.38 (s, 18H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=155.54, 77.71, 60.80, 52.92, 28.23.
Step 1: To a solution of trans-X26 (0.20 g, 0.57 mmol, 1.0 eq.) in MeOH (6.5 mL) was added Pd/C (10%, 39 mg). The mixture was stirred at r.t. under H2-atmosphere at 5 bar for 20 h. The catalyst was removed by filtration over Kieselgur and the solvent was evaporated to give (trans)-1,4-bis(benzyloxy)butane-2,3-diamine as a colourless oil.
Step 2: The material obtained in step 1 was treated according general protocol A. The crude product was purified by FCC (isohexanes/EtOAc) to give di-tert-butyl (trans)-1,4-bis(benzyloxy)butane-2,3-diyl)dicarbamate as a colourless solid.
Step 3: A solution of the material obtained in step 2 in MeOH (2 mL) was added Pd/C (10%, 30 mg). The mixture was stirred at r.t. under H2-atmosphere at 40 bar for 43 h. The catalyst was removed by filtration over Kieselgur, the solvent was evaporated under reduced pressure and the crude was purified by FCC (isohexane/EtOAc) to give trans-X27 as a colourless solid (59 mg, 0.18 mmol, 59% over 3 steps).
Rf=0.38 (EtOAc). HRMS (E1): for C14H29N2O6: calc. 321.2020. found: 321.2029. 1H-NMR (400 MHz, CDCl3): δ (ppm)=4.90 (d, J=8.3 Hz, 2H), 3.86 (s, 2H), 3.80-3.71 (m, 4H), 3.50 (s, 2H), 1.44 (s, 18H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=157.3, 80.4, 62.9, 52.6, 28.4.
Step 1: Compound cis-X27 (500 mg, 1.56 mmol) was treated with MsCl (0.278 mL, 0.359 mmol, 2.3 equiv.) in pyridine (15 mL, 0.1 M) at 0° C. to give (cis)-S,S′-(2,3-bis((tert-butoxycarbonyl)amino)butane-1,4-diyl) dimesylate (680 mg, 1.43 mmol, 92%) as a colourless solid after precipitation of the reaction mixture from cold water and filtration of the solids.
Step 2: The material obtained in step 1 (600 mg, 1.26 mmol) was treated according to general protocol B, to yield (cis)-S,S′-(2,3-bis((tert-butoxycarbonyl)amino)butane-1,4-diyl) diethanethioate, which was isolated through FCC (isohexane/EtOAc) (27 mg, 0.06 mmol, 5%).
Step 3: The thioacetylated intermediate obtained above (600 mg, 1.26 mmol) was treated according to general protocol C, to yield cis-X28, which was isolated after FCC (isohexane/EtOAc) (18 mg, 0.05 mmol, 82%).
Rf=0.45 (isohexanes:EtOAc, 6:1). HRMS (ESI)+: for C14H26N2O4S2: calc. 350.1407. found: 351.1411. 1H-NMR (400 MHz, CDCl3): δ (ppm)=5.55-5.20 (br-s, 2H), 4.42-3.72 (br-m, 2H), 3.54-3.08 (br-m, 2H), 2.88 (br-dd, J=13.4, 7.9 Hz, 2H), 1.45 (s, 18H). 13C-NMR (101 MHz, CDCl3): b (ppm)=156.0, 80.2, 52.7, 40.8, 28.3.
Step 1: Compound trans-X27 (320 mg, 1.00 mmol) was treated with MsCl (0.18 mL, 2.3 mmol, 2.3 equiv.) in pyridine (10 mL, 0.1 M) at 0° C. to give (trans)-S,S′-(2,3-bis((tert-butoxycarbonyl)amino)butane-1,4-diyl) dimesylate (312 mg, 0.66 mmol, 66%) as a colourless solid after precipitation of the reaction mixture from cold water and filtration of the solids.
Step 2: The dimesyl species (0.28 g, 0.59 mmol) was treated according to general protocol B. to give (trans)-S,S′-(2,3-bis((tert-butoxycarbonyl)amino)butane-1,4-diyl) diethanethioate (0.23 g, 0.53 mmol, 90%) as a colourless solid after purification by FCC (isohexanes/EtOAc).
Step 3: The material obtained in step 2 (0.23 g, 0.52 mmol) was treated according to general protocol C, to yield trans-X28, which was precipitated from the methanolic solution and isolated by filtration (0.18 g, 0.51 mmol, 57% over 3 steps).
Rf=0.29 (isohexane:EtOAc, 9:1). HRMS (EI): for C14H26N2O4S2: calc. 350.1334. found: 350.1331. 1H-NMR (400 MHz, CDCl3): δ (ppm)=5.04 (s, 2H), 3.68 (s, 2H), 3.12 (s, 2H), 2.85 (s, 2H), 1.43 (s, 18H). 11C-NMR (101 MHz, CDCl3): δ (ppm)=156.0, 80.2, 55.4, 40.5, 28.5.
cis-X31 (1.19 g, 2.2 mmol) was deprotected in THE (44 mL, 0.05 M) using NaOMe (1.0 mL, 5.4 M in MeOH, 5.5 mmol). After quantitative deprotection, the dihydrochloride cis-X29 was precipitated from the reaction mixture using a solution of HCl (5.5 mL, 4 M in dioxane, 22 mmol). After filtration, the cis-X29 was isolated as a yellow solid (0.54 g, 2.15 mmol, 99%).
HRMS (ESI): C6H13N2O2S2: calc. 177.05147. found 177.05147.
Step 1: Deprotection of trans-X28 (170 mg, 0.49 mmol) was achieved according to general protocol D to give (trans)-1,2-dithiane-4,5-diamine dihydrochloride (106 mg, 0.48 mmol, 98%) as a colourless solid.
Step 2: (trans)-1,2-dithian-4,5-diamine dihydrochloride (50.1 mg, 0.22 mmol) was dissolved in MeOH (0.01 M) and KOH (0.5 mL, 1 M solution in MeOH) was. To the mixture was added a 0.88 M solution of glyoxal (280 μL, 9:1 MeOH/H2O) and the mixture was stirred at r.t. for 1 h, before a solution of NaCNBH3 (672 μL, 1 M in solution in MeOH) was added and the mixture was further stirred at r.t. for 1 h. The mixture was filtered over Kieselgur and washed with MeOH (20 mL), before being concentrated under reduced pressure. The residue was taken into MeOH (0.5 mL) and then added dropwise to a solution of HCl in Et2O (0.01 M, 12 mL), the solid precipitated was filtered off and dried under reduced pressure to give trans-X29 as a colourless powder (50.3, 0.20 mmol, 90%).
trans-X31 (1.90 g, 3.4 mmol) was deprotected in THE (40 mL, 0.085 M) using NaOMe (1.6 mL, 5.4 M in MeOH, 8.6 mmol) in THF (9.0 mL, 1 M). After quantitative deprotection, the dihydrochloride trans-X29 was precipitated from the reaction mixture using a solution of HCl (8.6 mL, 4 M in dioxane, 34 mmol). After filtration, the trans-X29 was isolated as a yellow solid (0.83 g, 3.3 mmol, 97%).
HRMS (ESI): C6H13N2O2S2: calc. 177.05147. found 177.05160. 1H-NMR (400 MHz, D2O): δ (ppm)=3.96-3.83 (m, 1H), 3.86-3.75 (m, 1H), 3.55 (t, J=10.8 Hz, 1H), 3.39-3.24 (m, 2H). 13C-NMR (101 MHz, D2O): δ (ppm)=56.6, 40.9, 35.6.
Closely following the procedure from Okumura et al., J. Am. Chem. Soc., 2021, 143, 11, 4112, trans-2-butene-1,4-diol (0.187 mL, 2.27 mmol) was dissolved in MeCN (12 mL, 0.2 M) and solid 2-nitrobenzenesulfonamide (1.01 mg, 4.99 mmol), sodium hypochlorite pentahydrate (822 mg, 4.99 mmol) and iodine (58.0 mg, 0.23 mmol) were added and the resulting mixture was stirred at r.t. for 3 h, was then heated to 70° C. and further stirred for 12 h. To the reaction mixture was added 0.5 M aq. Na2S2O3 (40 mL, 1 M aq. solution) and EtOAc (50 mL) and the organic layer was washed with aqueous NaCl (2×20 mL). The aqueous phase was extracted with EtOAc (4×30 mL), the combined organic layers were dried over Na2SO4 and concentrated under reduced pressure, yielding cis-X38 in good purity without further purification (562 mg, 1.14 mmol, 50%).
HRMS (ESI): C16H17N4O10S2: calc. 489.03916. found 489.04007. 1H-NMR (400 MHz, DMSO-d6): δ (ppm)=8.08-8.02 (m, 2H), 8.00-7.93 (m, 2H), 7.85-7.78 (m, 4H), 7.63-7.51 (m, 2H), 4.40 (s, 2H), 3.55 (p, J=5.3 Hz, 2H), 3.41 (h, J=5.6 Hz, 4H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=147.2, 133.8, 133.7, 132.7, 130.0, 124.5, 60.3, 56.5.
Closely following the procedure from Okumura et al., J. Am. Chem. Soc., 2021, 143, 11, 4112, cis-2-butene-1,4-diol (0.200 mL, 2.50 mmol) was dissolved in MeCN (15.0 mL, 0.2 M) and solid 2-nitrobenzenesulfonamide (1.10 mg, 5.30 mmol), sodium hypochlorite pentahydrate (880 mg, 5.30 mmol) and iodine (62.0 mg, 0.24 mmol) were added and the resulting mixture was stirred at r.t. for 12 h, was then heated to 70° C. and further stirred for 12 h. To the reaction mixture was added 0.5 M aqueous Na2S2O3 (40 mL, 1 M aq. solution) and EtOAc (50 mL) and the organic layer was washed with aqueous NaCl (2×20 mL). The aqueous phase was extracted with EtOAc (4×30 mL), the combined organic layers were dried over MgSO4 and concentrated under reduced pressure, yielding trans-X38 in good purity without further purification (1.1 g, 2.2 mmol, 91%).
1H-NMR (400 MHz, DMSO-d6): δ (ppm)=8.05-8.01 (m, 2H), 7.99-7.94 (m, 2H), 7.88-7.78 (m, 4H), 7.64 (d, J=8.5 Hz, 2H), 4.56 (t, J=5.0 Hz, 2H), 3.57 (q, J=6.4 Hz, 2H), 3.33 (dt, J=10.5, 5.2 Hz, 2H), 3.24 (dt, J=10.9, 5.6 Hz, 2H).
Step 1: N,N′-(syn)-1,4-dihydrobutane-2,3-diyl)bis(2-nitrobenzenesulfonamide) cis-X38 (722 mg, 1.47 mmol) was dissolved in pyridine (10 mL, 0.15 M) and reacted with MsCl (356 μL, 4.6 mmol). After precipitation from water and washings with diethylether, the targeted dimesylate (syn)-2,3-bis((2-nitrophenyl)sulfonamido)butane-1,4-diyl dimethanesulfonate was received as a colourless solid and in excellent purity (694 mg, 1.07 mmol, 73%).
Step 2: The material obtained in step 1 (694 mg, 1.07 mmol) was suspended in acetone (21 mL, 0.05 M) and KSAc (366 mg, 3.21 mmol, 3.0 eq.) was added in one portion. The mixture was stirred at r.t. for 3 h, before being poured into water (50 mL). The solid was collected and azeotropically dried using toluene to yield the bis-thioacetate (syn)-2-((2-nitrophenyl)sulfonamido)-3-(((2-nitrophenyl)sulfonyl)methyl)butane-1,4-diyl) diethanethioate as a colourless solid (579 mg, 0.96 mmol, 90%).
Step 3: The material obtained in step 2 (572 mg, 0.95 mmol) was suspended in MeOH (35 mL, 0.03 M) and a solution of HCl (4.0 M in dioxane, 11.9 mL, 47.7 mmol) was added in one portion. The mixture was heated to 70° C. and was stirred for 15 h, before being cooled to r.t. A solution of iodine (242 mg, 0.95 mmol) in MeOH (3.2 mL, 0.3 M) was slowly added to the reaction mixture, swiftly causing the formation of orange precipitates, which was followed by the addition of water (50 mL). The solid precipitates were filtered, washed with water and diethylether, and were dried on high vacuum overnight, giving the cis-X39 as an orange solid (484 mg, 0.93 mmol, 98%).
Rf=0.25 (DCM). HRMS (ESI): C16H15N4O8S4—: calc. 518.97782. found 518.97835. 1H-NMR (400 MHz, DMSO-d6): δ (ppm)=8.15 (d, J=6.6 Hz, 2H, N—H), 8.06 (d, J=5.4 Hz, 2H), 8.00-7.92 (m, 2H), 7.89-7.79 (m, 4H), 3.76 (br-s, 2H), 3.46 (br-s, 4H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=147.4, 134.4, 132.9, 129.8, 124.5, 54.9, 53.6.
Step 1: N,N′-(anti)-1,4-dihydrobutane-2,3-diyl)bis(2-nitrobenzenesulfonamide) trans-X38 (12.0 g, 25.0 mmol) was dissolved in pyridine (160 mL, 0.25 M,) and reacted with MsCl (6.6 mL, 86 mmol, 3.5 equiv.). After precipitation from water and washings with diethylether, the targeted dimesylate (trans)-2,3-bis((2-nitrophenyl)sulfonamido)butane-1,4-diyl dimethane-sulfonate was received as a colourless solid and in excellent purity (12.0 g, 19.0 mmol, 76%).
Step 2: The material obtained in step 1 (500 mg, 0.77 mmol) was suspended in acetone (20 mL, 0.05 M,) and KSAc (0.27 g, 2.3 mmol, 3.0 equiv.) was added in one portion. The mixture was stirred at r.t. for 1 h, during which the heterogeneous mixture significantly thickened due to the formation of insoluble KOMs. The reaction mixture was concentrated to ca. 20% of its volume and the remaining mixture was partitioned between saturated aqueous NaCl and EtOAc. The aqueous layer was extracted with EtOAc (3×100 mL), and the combined organic layers were washed with saturated aqueous NaCl, dried over MgSO4 and concentrated. The orange crude solid was directly used for further transformations (460 mg, 0.75 mmol, 97%).
Step 3: The material obtained in step 2 (2.90 g, 4.8 mmol) was suspended in MeOH (0.1 M, 50 mL) and charged with HCl (4 M in dioxane, 24 mL) at r.t. The reaction mixture was stirred for 16 h, upon which the mixture turned homogeneous. Next, a solution of iodine (1.2 g, 4.8 mmol) in MeOH (17 mL, 0.3 M,) was slowly added to the reaction mixture, swiftly causing the formation of orange precipitates, which was followed by the addition of water (200 mL). The solid precipitates were filtered, washed with water and diethylether, and were concentrated under reduced pressure giving trans-X39 as an orange solid (2.4 g, 4.5 mmol, 94%).
Rf=0.26 (DCM). HRMS (ESI): C16H15N4O8S4: calc. 518.97782. found 518.9791. 1H-NMR (400 MHz, DMSO-d6): δ (ppm)=8.14 (br s, 2H), 8.00 (dt, J=7.8, 3.4 Hz, 2H), 7.94-7.88 (m, 2H), 7.86-7.78 (m, 4H), 3.60-3.48 (m, 2H), 2.95 (br s, 4H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=147.5, 134.5, 133.4, 130.2, 124.9, 56.9, 40.7.
N,N′-(syn)-1,2-dithiane-4,5-diyl)bis(2-nitrobenzenesulfonamide) cis-X39 (484 mg, 0.93 mmol) was dissolved in anhydrous DCM (18 mL, 0.05 M), DIPEA (576 μL, 3.25 mmol) was added and the mixture was cooled to 0° C. A solution of vinyl-thianthrenium tetrafluoroborate (371 mg, 1.17 mmol) in DCM (0.3 M) was added dropwise and the resulting mixture was stirred for 15 min, was then allowed to warm to r.t. and was further stirred at r.t. for 3 h. The mixture was concentrated and purified using FCC (hexanes/DCM 1:1→DCM) to yield cis-X40 as a colourless solid (291 mg, 0.53 mmol, 57%).
Rf=0.56 (DCM). HRMS (ESI): C19H19N4O10S4: calc. 590.99895. found 590.99972. 1H-NMR (400 MHz, DMSO-d6): δ (ppm)=8.05 (d, J=7.7 Hz, 2H), 7.93 (d, J=7.0 Hz, 2H), 7.87 (t, J=7.3 Hz, 2H), 7.76 (t, J=7.6 Hz, 2H), 4.18 (d, J=7.0 Hz, 2H, N—H), 3.74-3.58 (m, 4H), 3.57-3.46 (m, 2H), 3.07 (d, J=13.9 Hz, 2H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=147.4, 135.3, 132.7, 130.3, 124.5, 54.1, 42.3, 36.8.
N,N′-(trans)-1,2-dithiane-4,5-diyl)bis(2-nitrobenzenesulfonamide) trans-X39 (240 mg, 0.47 mmol) was dissolved in anhydrous DCM (9.5 mL, 0.05 M), DIPEA (290 μL, 1.60 mmol) was added and the mixture was cooled to 0° C. A solution of vinyl-thianthrenium tetrafluoroborate (200 mg, 0.60 mmol) in DCM (0.3 M) was added dropwise and the resulting mixture was stirred for 15 min, was then allowed to warm to r.t. and was further stirred at r.t. for 3 h. The mixture was concentrated and purified using FCC (hexanes/DCM 1:1→DCM) to yield trans-X40 as a colourless solid (230 mg, 0.42 mmol, 90%).
Rf=0.54 (DCM). HRMS (ESI): C18H22N50S4: calc. 564.0351. found 564.0344. 1H-NMR (400 MHz, DMSO-d6): δ (ppm)=8.10-8.04 (m, 2H), 7.96 (dd, J=7.8, 1.5 Hz, 2H), 7.89-7.80 (m, 4H), 4.27-4.15 (m, 2H), 3.98-3.86 (m, 2H), 3.74-3.59 (m, 2H), 3.56-3.42 (m, 2H), 3.00 (d, J=12.9 Hz, 2H). 93C-NMR (101 MHz, DMSO-d6): δ (ppm)=147.1, 134.7, 133.0, 129.9, 124.5, 60.8, 43.2, 38.5.
Compounds of the invention: Fluorogenic Probes
6-chloro-2-(5-chloro-2-hydroxyphenyl)quinazolin-4(3H)-one (PQ-OH) (70.0 mg, 0.228 mmol) (Felber, J. et al. ChemRxiv 2020. https://doi.org/10.26434/chemrxiv.13483155.v1) was coupled to compound X9 (57.9 mg, 0.273 mmol) according to general protocol E. Purification by FCC (isohexane/EtOAc) gave compound P1 (75.0 mg, 0.148 mmol, 65%) as a colourless solid.
Rf=0.44 (isohexane:EtOAc, 2:1). HRMS (ESI+): for C22H20C12N3O3S2: calc. 508.03176. found 508.03187. Individual rotamers were observed by NMR spectroscopy at 298 K (ratio ca. 1:1) and time-averaged spectra were obtained at 373 K. 1H-NMR (400 MHz, tetrachlorethane-d2, 373 K): δ (ppm)=8.23 (t, J=1.5 Hz, 1H), 7.95 (d, J=2.6 Hz, 1H), 7.76-7.67 (m, 1H), 7.49 (dd, J=8.7 Hz, J=2.6 Hz, 1H), 7.15 (d, J=8.7 Hz, 1H), 3.77 (ddd, J=13.6 Hz, J=6.8 Hz, J=3.3 Hz, 1H), 3.72 (ddd, J=11.0 Hz, J=10.5 Hz, J=3.2 Hz, 1H), 3.38 (ddd, J=14.0 Hz, J=10.2 Hz, J=5.5 Hz, 1H), 3.11 (dd, J=12.9 Hz, J=10.3 Hz, 1H), 3.03 (dd, J=13.0 Hz, J=3.3 Hz, 1H), 2.87 (dd, J=13.6 Hz, J=10.7 Hz, 1H), 2.75 (dd, J=13.5 Hz, J=3.0 Hz, 1H), 2.07 (ddd, J=13.5 Hz, J=10.7 Hz, 1H), 1.86 (ddd, J=11.3 Hz, J=7.6 Hz, 1H), 1.70 (ddd, J=12.4 Hz, J=3.4 Hz, 1H), 1.66-1.55 (m, 1H), 1.39-1.22 (m, 1H). 13C-NMR (101 MHz, tetrachlorethane-d2, 373 K): δ (ppm)=160.4, 153.3, 149.0, 147.1, 135.3, 133.2, 132.3, 131.9, 130.3, 129.5, 128.0, 125.8, 125.3, 121.9, 63.1, 40.7, 40.2, 39.5, 36.2, 26.9, 23.1.
PQ-OH (51.6 mg, 0.168 mmol) was coupled to compound X12 (29.6 mg, 0.140 mmol) according to general protocol E. Purification by FCC (isohexane/EtOAc) gave compound P2 (28.4 mg, 0.056 mmol, 40%) as a colourless solid.
Rf=0.40 (isohexane:EtOAc, 2:1). HRMS (ESI+): C22H20C12N3O3S2: calc. 508.03176. found 508.03186. Individual rotamers were observed by NMR spectroscopy at 298 K (ratio ca. 1:1) and time-averaged spectra were obtained at 373 K. 1H-NMR (400 MHz, tetrachlorethane-d2, 373 K): δ (ppm)=9.72 (s, 1H), 8.23 (t, J=1.5 Hz, 1H), 7.99 (d, J=2.6 Hz, 1H), 7.73 (m, 2H), 7.50 (dd, J=8.7 Hz, J=2.6 Hz, 1H), 7.17 (d, J=8.7 Hz, 1H), 4.48 (d, J=11.7 Hz, 1H), 4.07 (d, J=12.5 Hz, 1H), 3.53-3.33 (m, 2H), 3.18-2.88 (m, 1H), 2.77 (dd, J=14.0 Hz, J=3.0 Hz, 1H), 2.43 (d, J=12.7 Hz, 1H), 2.28 (dd, J=12.7 Hz, J=4.0 Hz, 1H), 2.25-2.17 (m, 1H), 1.81 (d, J=13.6 Hz, 1H), 1.70-1.54 (m, 1H), 1.47 (d, J=13.1 Hz, 1H). 13C-NMR (101 MHz, tetrachlorethane-d2, 373 K): δ (ppm)=160.2, 154.9, 149.1, 147.7, 147.5, 135.3, 133.5, 132.4, 132.3, 130.5, 129.9, 128.0, 126.1, 125.4, 122.4, 54.6, 42.2, 41.3, 36.0, 29.7, 25.3, 23.1.
3′-hydroxy-6′-methoxy-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (MF-OH) (38.1 mg, 0.11 mmol) was coupled to compound X9 (21.2 mg, 0.10 mmol) according to general protocol E. Purification by FCC (isohexane/EtOAc) gave compound P3 (27.1 mg, 0.05 mmol, 50%) as a colourless solid.
Rf=0.58 (isohexane:EtOAc, 1:1). HRMS (ESI+) C29H26NO6S2: calc. 548.11961. found 548.11973. 1H-NMR (500 MHz, CDCl3): δ (ppm)=8.02 (d, J=7.1 Hz, 1H), 7.67 (tt, J=7.5, 1.4 Hz, 1H), 7.62 (t, J=7.3 Hz, 1H), 7.15 (d, J=7.4 Hz, 1H), 7.09 (dd, J=3.7, 1.7 Hz, 1H), 6.84-6.77 (m, 2H), 6.76 (d, J=2.5 Hz, 1H), 6.70 (d, J=8.8 Hz, 1H), 6.62 (dd, J=8.8, 2.5 Hz, 1H), 3.94-3.84 (m, 1H), 3.84 (s, 3H), 3.80 (dd, J=10.1, 3.4 Hz, 1H), 3.35 (d, J=15.2 Hz, 1H), 3.20-3.06 (m, 2H), 2.94 (dd, J=13.5, 10.8 Hz, 1H), 2.81 (dd, J=13.5, 2.7 Hz, 1H), 2.23-2.09 (m, 1H), 1.96 (dt, J=19.0, 8.5 Hz, 1H), 1.84-1.72 (m, 1H), 1.72-1.60 (m, 1H), 1.48-1.36 (m, 1H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=169.5, 161.5, 153.4, 153.2, 152.6, 152.4, 152.0, 135.2, 130.0, 129.2, 129.1, 126.6, 125.2, 124.2, 117.6, 116.4, 112.0, 111.0, 110.4, 101.0, 82.7, 63.1, 55.7, 41.5, 41.1, 40.7, 37.0, 34.3, 29.2, 27.8, 27.2, 26.0, 24.0, 23.1.
MF-OH (25.1 mg, 0.07 mmol) was coupled to compound X12 (14.0 mg, 0.07 mmol) according to general protocol E. Purification by FCC (isohexane/EtOAc) gave compound P4 (11.0 mg, 0.02 mmol, 30%) as a colourless solid.
Rf=0.66 (isohexane:EtOAc, 1:1). HRMS (ESI+) C29H26NO6S2: calc. 548.11961. found 548.11996. 1H-NMR (500 MHz, CDCl3): δ (ppm)=8.02 (d, J=7.4 Hz, 1H), 7.67 (t, J=7.3 Hz, 1H), 7.62 (t, J=7.3 Hz, 1H), 7.16 (d, J=7.5H, 1H), 7.12-7.01 (m, 1H), 6.82-6.77 (m, 2H), 6.76 (d, J=2.4 Hz, 1H), 6.70 (d, J=8.8 Hz, 1H), 6.62 (dd, J=8.8, 2.4 Hz, 1H), 4.56 (t, J=10.9 Hz, 1H), 4.14 (t, J=12.5 Hz, 1H), 3.84 (s, 3H), 3.62-3.42 (m, 2H), 2.97 (dt, J=44.8, 12.0 Hz, 1H), 2.83 (t, J=15.1 Hz, 1H), 2.51 (ddt, J=10.9, 7.0, 4.1 Hz, 1H), 2.39-2.21 (m, 2H), 1.86 (d, J=13.4 Hz, 1H), 1.74-1.59 (m, 1H), 1.52 (d, J=11.9 Hz, 1H), 1.36-1.21 (m, 1H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=169.5, 161.5, 153.2, 152.7, 152.4, 152.0, 135.2, 130.0, 129.2, 129.0, 126.6, 125.2, 124.2, 117.6, 116.3, 112.0, 111.0, 110.4, 101.0, 82.7, 55.7, 54.1, 53.3, 42.3, 42.2, 40.7, 40.2, 36.2, 35.9, 30.1, 29.4, 25.5, 25.0, 23.6.
PQ-OH (67.9 mg, 0.221 mmol) was coupled to compound trans-X29 (50 mg, 0.201 mmol) according to general protocol E. Purification by FCC (DCM/MeOH) gave compound P5 (50.4 mg, 0.099 mmol, 49%) as a colourless solid. Further purification was achieved by preparative HPLC (H2O/MeCN, 0.1% FA) to yield P5 as a colourless solid.
Rf=0.29 (DCM:MeOH, 9:1). HPLC RT=5.80 min (LRMS: m/z 508.9). HRMS (ESI): C21H19C12N4O3S2: [M+H] calc. 509.02701. found 509.02701. 1H-NMR (400 MHz, MeOD-d4): δ (ppm)=8.22 (d, J=2.4 Hz, 1H), 7.91-7.85 (m, 2H), 7.77 (d, J=8.7 Hz, 1H), 7.65 (dd, J=8.8, 2.6 Hz, 1H), 7.41 (d, J=8.8 Hz, 1H), 3.89 (d, J=15.1 Hz, 2H), 3.61-3.44 (m, 1H), 3.14 (td, J=9.8, 4.3 Hz, 1H), 3.09-2.95 (m, 4H), 2.96-2.84 (m, 2H). 13C-NMR (101 MHz, MeOD-d4): δ (ppm)=154.3, 136.3, 134.2, 133.0, 132.4, 131.0, 130.3, 126.5, 126.2, 65.0, 57.1, 44.9, 40.7, 38.2, 38.1.
P5 (9.4 mg 0.018 mmol) was dissolved in MeOH (5 mM) and a solution of formaldehyde (0.88 M, 10:1 diluted from 37% aq. solution: 209 μL, 0.184 mmol) was added. After 5 min of stirring at r.t., a solution of NaCNBH3 (0.1 M solution in MeOH: 184 μL, 0.018 mmol) was added. The mixture was stirred at r.t. for 10 min, before being purified by preparative HPLC (H2O/MeCN, 0.1% FA) to yield P6 as a colourless solid (5.0 mg, 53%).
Rf=0.40 (DCM:MeOH, 9:1). HRMS (ESI+): C22H21C12N4O3S2: calc. 523.04266. found 523.04279. 1H-NMR (400 MHz, MeOD-d4): δ (ppm)=8.40 (s, 1H), 8.10 (d, J=2.4 Hz, 1H), 7.76 (dd, J=8.7, 2.5 Hz, 1H), 7.70 (d, J=2.6 Hz, 1H), 7.65 (d, J=8.7 Hz, 1H), 7.53 (dd, J=8.8, 2.6 Hz, 1H), 7.29 (d, J=8.8 Hz, 1H), 3.74 (dd, J=12.6, 5.1 Hz, 1H), 3.08 (d, J=10.7 Hz, 1H), 3.07-3.02 (m, 2H), 2.94-2.84 (m, 1H), 2.82 (d, J=13.2 Hz, 1H), 2.75 (t, J=10.6 Hz, 1H), 2.74-2.65 (m, 1H), 2.47-2.40 (m, 1H), 2.05 (s, 3H).
A solution of pyridine (49 μL, 1 M in anhydrous DCM) was added to a solution P5 (6.0 mg 11.8 μmol) in anhydrous DCM (0.01 M), followed by addition of a solution AcCl (29 μL, 1 M in anhydrous DCM). The mixture was stirred at r.t. for 30 min, was then diluted with DCM (5 mL), before being poured into aq. NH4Cl solution (10 mL). The organic layer was washed with sat. aq. NH4Cl (2×5 mL), the aq. layer was extracted with DCM (2×5 mL). The combined organic layers were dried over Na2SO4, filtered and concentrated under reduced pressure to yield P7 as a colourless solid (6.2 mg, 11.2 μmol, 95%).
Rf=0.33 (EtOAc). HRMS (ESI+): C23H21C12N4O4S2: calc. 551.03758. found 551.03821. 1H-NMR (400 MHz, CDCl3): 10.16 (s, 1H), 8.24 (d, J=2.3 Hz, 1H), 7.97 (s, 1H), 7.76 (dd, J=8.7, 2.2 Hz, 1H), 7.72 (d, J=8.7 Hz, 1H), 7.53 (dd, J=8.7, 2.5 Hz, 1H), 7.26-7.03 (m, 1H), 4.73-4.32 (m, 1H), 4.23-3.87 (m, 2H), 3.76 (s, 2H), 3.69-3.42 (m, 3H), 3.28 (d, J=12.4 Hz, 1H), 3.14 (d, J=13.7 Hz, 1H), 2.10 (s, 3H).
A solution of pyridine (49 μL, 1 M in anhydrous DCM) was added to a solution P5 (6.0 mg 11.8 μmol) in anhydrous DCM (0.01 M), followed by addition of a solution methyl chloroformate (29 μL, 1 M in anhydrous DCM). The mixture was stirred at r.t. for 30 min, was then diluted with DCM (5 mL), before being poured into aq. NH4Cl solution (10 mL). The organic layer was washed with sat. aq. NH4Cl (2×5 mL), the aq. layer was extracted with DCM (2×5 mL). The combined organic layers were dried over Na2SO4, filtered and concentrated under reduced pressure to yield P8 as a colourless solid (6.1 mg, 10.7 μmol, 91%).
Rf=0.52 (isohexane:EtOAc, 1:2). HRMS (ESI+): C23H21Cl2N4O5S2: calc. 567.03249. found 567.03323. 1H-NMR (400 MHz, CDCl3): δ (ppm)=9.99 (s, 1H), 8.26 (d, J=2.1 Hz, 1H), 7.97 (s, 1H), 7.75 (dd, J=8.7, 2.3 Hz, 1H), 7.72 (d, J=8.7 Hz, 1H), 7.53 (dd, J=8.7, 2.6 Hz, 1H), 7.17 (d, J=8.7 Hz, 1H), 4.11-4.01 (m, 1H), 4.02-3.84 (m, 2H), 3.77 (d, J=18.4 Hz, 2H), 3.71 (s, 3H), 3.62-3.47 (m, 2H), 3.34 (s, 1H), 3.23 (dd, J=13.4, 2.5 Hz, 1H), 3.16 (d, J=12.2 Hz, 1H).
A solution of pyridine (49 μL, 1 M in anhydrous DCM) was added to a solution P5 (5.0 mg 9.8 μmol) in anhydrous DCM (0.01 M), followed by addition of a solution methane sulfonyl chloride (29 μL, 1 M in anhydrous DCM). The mixture was stirred at r.t. for 30 min, was then diluted with DCM (5 mL), before being poured into aq. NH4Cl solution (10 mL). The organic layer was washed with sat. aq. NH4Cl (2×5 mL), the aq. layer was extracted with DCM (2×5 mL). The combined organic layers were dried over Na2SO4, filtered and concentrated under reduced pressure to yield P9 as a colourless solid (3.5 mg, 10.7 μmol, 61%).
Rf=0.49 (isohexane:EtOAc, 1:1). HRMS (ESI+): C22H21C12N4O5S2: calc. 587.00456. found 587.00505.
MF-OH (69.6 mg, 0.19 mmol) was coupled to compound trans-X29 (150 mg, 0.28 mmol) according to general protocol E. Purification by FCC (DCM/MeOH) and subsequently by preparative HPLC (MeCN/H2O/0.1% formic acid) gave compound P12 (31.0 mg, 0.06 mmol, 31%) as a colourless solid.
Rf=0.52 (DCM:MeOH, 9:1). HRMS (ESI): C28H25N2O6S2: calc. 549.11485. found 549.11466. 1H-NMR (400 MHz, DMSO-de): δ (ppm)=8.04 (d, J=7.6 Hz, 1H), 7.81 (t, J=7.5 Hz, 1H), 7.75 (td, J=7.5, 0.8 Hz, 1H), 7.32 (d, J=7.6 Hz, 1H), 7.27 (t, J=2.5 Hz, 1H), 6.96-6.92 (m, 2H), 6.83 (d, J=8.7 Hz, 1H), 6.75 (dd, J=8.8, 2.5 Hz, 1H), 6.71 (dd, J=8.8, 0.9 Hz, 1H), 3.83 (s, 3H), 3.81-3.70 (m, 2H), 3.58-3.46 (m, 1H), 3.24 (dd, J=13.0, 10.7 Hz, 1H), 3.18-3.13 (m, 1H), 3.09-2.96 (m, 3H), 2.93 (dq, J=8.2, 3.0 Hz, 1H), 2.83 (dd, J=13.1, 10.6 Hz, 1H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=168.5, 161.2, 152.8, 152.5, 152.4, 151.6, 151.0, 135.9, 130.4, 129.1, 128.9, 125.7, 124.9, 124.0, 118.3, 115.9, 112.3, 110.6, 110.2, 100.8, 81.7, 63.9, 55.8, 55.7, 43.8, 36.7.
MF-OH (136.6 mg, 0.37 mmol) was coupled to compound cis-X29 (92.3 mg, 0.37 mmol) according to general protocol E. Purification by FCC (DCM/MeOH) and subsequently by preparative HPLC (MeCN/H2O/0.1% formic acid) gave compound P13 (29.2 mg, 0.05 mmol, 14%) as a colourless solid.
Rf=0.55 (DCM:MeOH, 9:1). HRMS (ESI): C28H25N2O6S2: calc. 549.11485. found 549.11458. 1H-NMR (400 MHz, CDCl3): δ (ppm)=8.03 (d, J=7.4 Hz, 1H), 7.67 (td, J=7.4, 1.2 Hz, 1H), 7.62 (t, J=7.2 Hz, 1H), 7.16 (d, J=7.5 Hz, 1H), 7.11-7.04 (m, 1H), 6.83-6.75 (m, 3H), 6.70 (d, J=8.8 Hz, 1H), 6.62 (dd, J=8.8, 2.5 Hz, 1H), 4.38 (ddt, J=11.5, 7.9, 3.5 Hz, 1H), 4.08-3.92 (m, 1H), 3.64 (dtd, J=32.4, 12.8, 12.2, 3.4 Hz, 1H), 3.50 (dt, J=14.0, 2.3 Hz, 1H), 3.39-3.29 (m, 1H), 3.27-2.93 (m, 4H), 2.64-2.52 (m, 1H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=169.5, 161.6, 153.2, 152.9, 152.4, 152.0, 135.3, 130.0, 129.2, 126.6, 125.2, 124.2, 117.5, 116.5, 112.0, 111.0, 110.4, 101.0, 82.7, 55.7, 52.9, 52.6, 52.4, 51.9, 45.3, 45.1, 42.2, 42.0, 40.9, 40.1, 29.8, 29.0.
P13 (4.0 mg 0.007 mmol) was dissolved in MeOH (5 mM) and a solution of formaldehyde (0.88 M, 10:1 diluted from 37% aq. solution: 166 μL, 0.146 mmol) was added. After 5 min of stirring at r.t., a solution of NaCNBH3 (0.5 M solution in MeOH: 29 μL, 0.015 mmol) was added. The mixture was stirred at r.t. for 10 min, before being purified by preparative HPLC (H2O/MeCN, 0.1% FA) to yield P14 as a colourless solid (3.3 mg, 0.006 mmol, 86%).
Rf=0.67 (DCM:MeOH, 9:1). HRMS (ESI): C29H27N2O6S2: calc. 563.13050. found 563.13047. 1H-NMR (400 MHz, CDCl3): δ (ppm)=8.02 (d, J=7.5 Hz, 1H), 7.67 (tt, J=7.5, 1.3 Hz, 1H), 7.62 (t, J=7.4 Hz, 1H), 7.16 (d, J=7.6 Hz, 1H), 7.14-7.04 (m, 1H), 6.83-6.75 (m, 4H), 6.70 (d, J=8.8 Hz, 1H), 6.63 (dd, J=8.8, 2.5 Hz, 1H), 4.46-4.29 (m, 1H), 4.02 (dd, J=23.5, 13.0 Hz, 1H), 3.97-3.86 (m, 1H), 3.84 (s, 3H), 3.52-3.41 (m, 1H), 3.33 (q, J=14.1, 13.0 Hz, 2H), 2.95 (d, J=7.7 Hz, 1H), 2.59-2.47 (m, 2H), 2.47-2.33 (m, 1H), 2.29 (s, 3H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=169.5, 161.6, 153.2, 152.8, 152.5, 152.4, 152.0, 135.2, 130.0, 129.2, 126.7, 125.2, 124.2, 117.5, 116.3, 112.0, 111.1, 110.4, 101.0, 82.7, 61.3, 56.1, 55.9, 55.8, 54.7, 53.8, 53.6, 41.8, 40.9, 40.2, 39.1, 38.9, 30.5, 29.8.
P13 (4.0 mg 0.007 mmol) was dissolved in anhydrous DCM (0.05 M) and DIPEA (0.5 M in anhydrous DCM: 29 μL, 0.146 mmol) was added. The mixture was cooled to 0° C. and a solution of AcCl (0.5 M in anhydrous DCM: 291 μL, 0.291 mmol) was added in a dropwise manner. The mixture was allowed to warm to r.t. and was further stirred for 1 h, before being concentrated under reduced pressure. Purification was achieved using FCC (EtOAc) to give P15 as a colourless solid (4.1 mg, 0.007 mmol, 99%).
Rf=0.29 (EtOAc). HRMS (ESI): C30H27N2O7S2: calc. 591.12542. found 591.12542. 1H-NMR (400 MHz, CDCl3): δ (ppm)=8.03 (d, J=7.4 Hz, 1H), 7.67 (t, J=7.4 Hz, 1H), 7.62 (t, J=7.3 Hz, 1H), 7.16 (d, J=7.4 Hz, 1H), 7.11 (s, 1H), 6.85-6.74 (m, 3H), 6.70 (d, J=8.8 Hz, 1H), 6.63 (dd, J=8.8, 2.3 Hz, 1H), 4.71 (s, 1H), 4.40 (s, 1H), 4.22 (s, 1H), 3.93-3.80 (m, 5H), 3.81-3.66 (m, 2H), 3.50 (dd, J=13.8, 9.1 Hz, 1H), 3.22-3.12 (m, 1H), 2.98 (d, J=10.7 Hz, 1H), 2.16 (s, 3H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=170.4, 169.4, 161.6, 153.4, 153.1, 152.4, 152.1, 152.1, 135.3, 130.0, 129.2, 126.6, 125.3, 124.2, 117.5, 116.9, 112.1, 111.0, 110.4, 101.0, 82.6, 55.2, 51.6, 47.0, 42.0, 37.0, 30.3, 22.0.
4-(4-methylpiperazin-1-yl)-4-oxobutanoic acid hydrochloride (20.7 mg, 0.088 mmol) was dissolved in anhydrous 1,2-dichloroethane (0.1 M) and DIPEA (31 μL, 0.175 mmol) and trimethylacetyl chloride (11 μL, 0.098 mmol) were added sequentially. The resulting mixture was stirred at r.t. for 2 h, before P13 (4.0 mg 0.007 mmol) in 1,2-dichloroethane (0.04 M) was added in one portion. The mixture was heated to 70° C. for 15 h. The mixture was concentrated under reduced pressure and purified using preparative HPLC (H2O/MeCN/0.1% formic acid) to yield P16 as a colourless solid (3.4 mg, 0.005 mmol, 71%).
Rf=0.40 (DCM:MeOH, 9:1). HRMS (ESI): C37H39N4O8S2: calc. 731.22038. found 731.21939. 1H-NMR (400 MHz, CDCl3): δ (ppm)=8.02 (d, J=7.5 Hz, 1H), 7.71-7.65 (m, 1H), 7.62 (t, J=7.4 Hz, 1H), 7.16 (d, J=7.5 Hz, 1H), 7.11 (dd, J=4.3, 2.0 Hz, 1H), 6.87-6.75 (m, 3H), 6.70 (dd, J=8.8, 1.1 Hz, 1H), 6.63 (dd, J=8.8, 2.5 Hz, 1H), 4.73 (s, 1H), 4.39 (s, 1H), 4.27 (s, 1H), 3.97 (s, 1H), 3.90 (d, J=10.6 Hz, 1H), 3.84 (s, 3H), 3.77 (s, 2H), 3.63 (s, 2H), 3.59-3.50 (m, 2H), 3.52-3.41 (m, 1H), 3.17 (d, J=13.3 Hz, 1H), 2.99 (d, J=11.1 Hz, 1H), 2.80-2.60 (m, 4H), 2.50-2.36 (m, 4H), 2.31 (s, 3H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=172.2, 170.1, 169.5, 161.6, 153.4, 153.2, 152.4, 152.2, 152.0, 135.3, 130.0, 129.2, 126.6, 125.3, 124.2, 123.2, 117.5, 116.8, 112.1, 111.1, 110.5, 101.0, 82.6, 55.8, 55.0, 54.7, 51.8, 46.0, 45.2, 41.8, 39.0, 35.8, 28.2.
Cysteamine hydrochloride X16 (1.00 g, 8.8 mmol) was dissolved in MeOH (30 mL) and the free base cysteamine was generated by addition of KOH (1.16 g, 17.6 mmol). Addition of methanolic I2 until a slight colour persisted permanently, followed by evaporation of all volatiles gave 2-(2-aminoethyldisulfanyl)ethanamine (cystamine) that was N-Boc protected according to general protocol A gave X17 (0.70 g, 2.0 mmol, 45%) as colourless crystalline needles after recrystallization from MeOH, that was directly used for the further steps.
Step 1: X17 (0.78 g, 2.21 mmol) was dissolved in anhydrous DMF (0.02 M) and solid NaH (2.2 eq) was added at 0° C., followed by addition of Mel (2.3 eq) in one portion. The mixture was stirred for 0.5 h, then allowed to warm to r.t. and stirred for a further 1 h. The mixture was diluted with EtOAc and washed with sat. aq. NaCl (2×). The combined aq. layers were extracted with EtOAc (3×50 mL) and the combined organic layers were dried over Na2SO4. filtered and concentrated and purified by FCC to afford di-tert-butyl (disulfanediylbis(ethane-2,1-diyl))bis(methylcarbamate) as a colourless solid.
Step 2: Deprotection of the material obtained in step 1 was achieved according to general protocol C and the resulting diamine dihydrochloride X18 (0.46 mg, 1.82 mmol, 82%) was obtained as a colourless solid.
1H-NMR (400 MHz, DMSO-d6): δ (ppm)=9.27 (s, 4H), 3.19 (d, J=7.4 Hz, 4H), 3.09 (dd, J=8.1, 5.7 Hz, 4H), 2.56 (s, 6H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=47.0, 32.4, 32.3.
PQ-OH (333 mg, 1.08 mmol) was coupled to X18 (715 mg, 2.82 mmol) according to general protocol E. Purification by FCC (DCM/MeOH) gave X19 as a colourless powder (245 mg, 0.477 mmol, 44%).
Rf=0.22 (DCM:MeOH, 9:1). HRMS (ESI+) for C21H23C12N4O3S2: calc. 513.05831. found 513.05886. 1H-NMR (400 MHz, tetrachlorethane-d2, 373 K): δ (ppm)=8.20 (t, J=1.5 Hz, 1H), 7.87 (d, J=2.6 Hz, 1H), 7.71 (d, J=1.5 Hz, 2H), 7.48 (dd, J=8.7, 2.6 Hz, 1H), 7.24 (d, J=8.7 Hz, 1H), 3.62 (s, 2H), 3.23 (d, J=6.2 Hz, 2H), 3.18 (d, J=6.1 Hz, 2H), 3.03 (s, 3H), 2.88 (s, 2H), 2.66 (s, 3H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=160.8, 153.1, 150.6, 147.9, 147.2, 134.9, 131.5, 131.4, 130.1, 129.6, 129.4, 128.5, 125.2, 125.0, 122.4, 47.7, 47.0, 35.1, 34.6, 32.4, 32.3.
Step 1: 5-hydroxy-1H-indole-2-carboxylic acid X20 (1.27 g, 7.15 mmol) was dissolved in MeOH (14 mL) and cooled to 0 C. SOCl2 (0.78 mL, 1.28 g, 10.7 mmol) was added dropwise and the resulting mixture was heated to 75° C. for 2 h, then cooled to 25° C. and diluted with EtOAc (100 mL). The organic layer was washed with sat. aq. NaHCO3 and sat. aq. NaCl and the combined aq. layers were extracted with EtOAc (3×50 mL). The combined organic layers were dried over Na2SO4, filtered, concentrated under reduced pressure and purified by FCC (isohexane/EtOAc) to give methyl 5-hydroxy-1H-indole-2-carboxylate X20a as a colourless powder (1.32 g, 6.9 mmol, 97%).
Step 2: PPh3 (125 mg, 0.52 mmol) was dissolved in THF (0.25 M) and cooled to 0° C. DIAD (94 μL, 0.48 mmol) was added dropwise and the mixture was stirred at r.t. for 30 min. A solution of 3-(azetidin-1-yl)propan-1-ol (0.5 M) in THF and a solution of methyl 5-hydroxy-1H-indole-2-carboxylate X20a (0.5 M) in THF were added subsequently. The mixture was allowed to warm to r.t. for 1 h, was then further stirred for 15 h, before being concentrated under reduced pressure and purified using FCC to give methyl 5-(3-(azetidin-1-yl)propoxy)-1H-indole-2-carboxylate (76 mg, 0.27 mmol, 62%) as a colourless oil.
Step 3: Methyl 5-(3-(azetidin-1-yl)propoxy)-1H-indole-2-carboxylate (222 mg, 0.77 mmol) was dissolved in MeOH:H2O (3:1, 40 mL) and solid KOH (61 mg, 0.92 mmol) was added. The resulting solution was heated to 75° C. for 15h, was cooled to r.t. and a solution of HCl (0.74 mL, 1.25 M in MeOH) was added. The resulting heterogenous mixture was concentrated under reduced pressure to obtain compound X21 (303 mg, 99%. 68% w/w calc. purity) contaminated with KCl. An analytically pure sample was separately obtained from RP chromatography (H2O/MeCN) to give X21 as a colourless powder.
HRMS (ESI−) for C13H13NO2Cl: calc. 275.13902. found 275.13910. 1H-NMR (400 MHz, DMSO-d6): δ (ppm)=11.14 (d, J=2.2 Hz, 1H), 7.25 (d, J=8.8 Hz, 1H), 7.08 (d, J=2.4 Hz, 1H), 6.76 (d, J=2.1 Hz, 2H), 6.74 (d, J=2.4 Hz, 1H), 4.02 (t, J=6.8 Hz, 2H), 3.63 (t, J=7.5 Hz, 4H), 2.91 (t, J=6.5 Hz, 2H), 2.20 (p, J=7.5 Hz, 2H), 1.86 (p, J=6.7 Hz, 2H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=165.1, 152.6, 134.1, 131.7, 127.6, 114.3, 112.9, 104.3, 102.6, 65.3, 53.2, 53.0, 25.4, 16.2.
Step 1: To a solution of Boc2O (6.4 g, 28.3 mmol) in DCM (1 M) was added dropwise at 0° C. a solution of 3-amino-1-propanol X30 (2.0 mL, 26.6 mmol) in anhydrous DCM (3 M). The reaction mixture was allowed to warm to r.t. and further stirred for 15 h, before a solution of BzCl (3.7 mL, 32 mmol) in DCM (3 M) was added and the resulting mixture was further stirred for 4 h, before being poured into a mixture of sat. aq. NH4Cl/H2O (1:1, 100 mL). The organic layer was separated and the aq. layer was extracted with DCM (3×50 mL), the combined organic layer were dried over Na2SO4, filtered, concentrated under reduced and purified by FCC (isohexane/EtOAc) to yield 3-((tert-butoxycarbonyl)amino)propyl benzoate as a colourless oil (6.2 g, 22.2 mmol, 83%).
Step 2: To the material obtained in step 1 (1.87 g, 6.69 mmol) in anhydrous DMF (0.02 M) at 0° C. was added iodomethane (0.5 mL, 8.03 mmol), directly followed by addition of solid NaH (60%, 0.295 g, 7.36 mmol), the mixture was then allowed to warm to r.t. and was further stirred for 2 h, before being poured into a mixture of sat. aq. NaCl/H2O (1:1, 100 mL). The organic layer was separated and the aq. layer was extracted with DCM (3×50 mL), the combined organic layer were dried over Na2SO4, filtered, concentrated under reduced pressure and purified by FCC (isohexane/EtOAc) to yield X32 as a colourless oil (1.28 g, 4.36 mmol, 65%).
Rf=0.47 (isohexane:EtOAc, 3:1). HRMS (ESI+) for C16H24NNaO4: calc 316.15193. found 316.15250. 1H-NMR (400 MHz, CDCl3): δ (ppm)=8.04 (d, J=7.1 Hz, 2H), 7.55 (t, J=7.4 Hz, 1H), 7.43 (t, J=7.6 Hz, 2H), 4.34 (t, J=6.3 Hz, 2H), 3.39 (t, J=6.9 Hz, 2H), 2.88 (s, 3H), 1.99 (p, J=6.5 Hz, 2H), 1.43 (s, 9H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=166.6, 155.8, 133.1, 130.3, 129.7, 128.5, 79.6, 62.8, 46.2, 33.9, 28.5, 27.5.
Step 1: To a solution of compound X31 (1.06 g, 3.61 mmol) in MeOH (11 mL) at 0° C. was added a freshly prepared solution of KOH in MeOH (1 M, 5.4 mL, 5.4 mmol), the reaction mixture was allowed to warm to r.t. and was further stirred for 3 h, before being concentrated under reduced pressure. The residue was taken into a mixture of sat. aq. NH4Cl/H2O (1:1, 50 mL) and extracted with EtOAc (3×50 mL) at pH<5 to yield intermediate tert-butyl (3-hydroxypropyl)(methyl)carbamate as a colourless oil (0.68 g, 3.59 mmol, 99%).
Step 2: PPh3 (911 mg, 3.47 mmol) was dissolved in THF (0.25 M) and cooled to 0° C. DIAD (701 μL, 3.57 mmol) was added dropwise and the mixture was stirred at r.t. for 30 min. A solution of the material obtained in step 1 containing tert-butyl (3-hydroxypropyl) (methyl)carbamate (632 mg, 3.31 mmol) in THF and a solution of the 5-methyl-1H-indole-2-carboxylate X20a (0.5 M) in THF were added subsequently. The mixture was allowed to warm to r.t. for 1 h, was then further stirred for 15 h, before being poured into a mixture of sat. aq. NaCl/H2O (1:1, 50 mL). The organic layer was separated and the aq. layer was extracted with EtOAc (3×50 mL), the combined organic layers were dried over Na2SO4, filtered, concentrated under reduced pressure and purified by FCC (isohexane/EtOAc) to yield X32 as a colourless solid (485 mg, 1.34 mmol, 40%).
Rf=0.56 (isohexane:EtOAc, 2:1). HRMS (ESI+) for C19H27N2NaO5: calc 385.17339. found 385.17382. 1H-NMR (400 MHz, CDCl3): δ (ppm)=8.94 (s, 1H), 7.31 (d, J=9.0 Hz, 1H), 7.14-7.10 (m, 1H), 7.05 (s, 1H), 6.99 (dd, J=8.9, 2.3 Hz, 1H), 4.00 (t, J=6.1 Hz, 2H), 3.93 (s, 3H), 3.43 (t, J=6.8 Hz, 2H), 2.88 (s, 3H), 2.02 (s, 2H), 1.43 (s, 9H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=162.5, 156.5, 156.0, 154.0, 132.4, 127.9, 127.6, 117.5, 112.9, 108.4, 103.5, 79.5, 65.6, 52.1, 46.2, 34.6, 29.7, 28.1.
To a solution of compound X32 (403 mg, 1.11 mmol) in MeOH/H2O (2:1, 60 mL) at r.t. was added solid KOH (780 mg, 11.1 mmol), the reaction mixture was heated to 80° C. and stirred for 2 h, before being cooled to r.t. and concentrated under reduced pressure. The residue was taken into a mixture of sat. aq. NH4Cl/H2O (1:1, 50 mL) and extracted with EtOAc (3×) at pH<5 to yield X33 as a colourless oil (384 mg, 1.1 mmol, 99%).
Rf=0.28 (DCM:MeOH, 9:1). 1H-NMR (400 MHz, CDCl3) δ (ppm)=40% 8.92 (s, 1H), 7.20 (s, 1H), 7.01 (d, J=9.9 Hz, 2H), 6.39 (s, 1H), 3.98 (s, 2H), 3.42 (s, 2H), 2.88 (s, 3H), 2.02 (s, 2H), 1.43 (s, 9H).
Compounds of the Invention: Therapeutic Proagents
Step 1: Following Tietze et al (Tietze, L. F. et al. ChemMedChem 2008, 3, 1946-1955. https://doi.org/10.1002/cmdc.200800250), to commercial (S)-3-(Boc)-5-(benzyloxy)-1-(chloromethyl)-1,2-dihydro-3H-benzo[e]indole X22 (591 mg, 1.39 mmol) were added dry THF (0.05 M), Pd/C (297 mg, 10% on charcoal), and aq. NH4HCO2 (2.8 mL of a 4 M aq. solution). The mixture was stirred for 80 min, filtered over Kieselgur and washed with EtOAc (20 mL) and the filtrates concentrated under reduced pressure to obtain (S)-3-(Boc)-1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indole as a colourless solid (460 mg, 1.38 mmol, 99%).
Step 2: (S)-3-(Boc)-1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indole (269 mg, 0.68 mmol) was coupled to X12 (144 mg, 0.68 mmol) according to general protocol E. Purification by FCC (isohexane/EtOAc) gave X23 as a colourless, crystalline solid (245 mg, 0.46 mmol, 67%).
Rf=0.50 (isohexane:EtOAc, 3:1). HRMS (ESI+) for C26H35N3O3ClS2: calc. 552.17520. found 552.17543. 1H-NMR (400 MHz, CDCl3): δ (ppm)=8.05 (s, 1H), 7.82-7.72 (m, 1H), 7.70 (d, J=8.4 Hz, 1H), 7.50 (t, J=7.6 Hz, 1H), 7.42-7.32 (m, 1H), 4.67 (dd, J=76.6, 12.0 Hz, 1H), 4.31 (dd, J=34.5, 12.9 Hz, 1H), 4.14 (t, J=10.4 Hz, 2H), 4.02 (t, J=10.0 Hz, 1H), 3.93 (d, J=11.4 Hz, 1H), 3.72-3.60 (m, 1H), 3.59-3.42 (m, 2H), 3.25-3.07 (m, 1H), 2.95 (dd, J=23.6, 12.1 Hz, 1H), 2.89-2.79 (m, 1H), 2.74-2.49 (m, 1H), 2.47-2.26 (m, 2H), 1.88 (d, J=16.1 Hz, 1H), 1.72 (s, 1H), 1.25 (s, 9H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=151.4, 147.3, 140.1, 129.2, 126.5, 123.3, 123.2, 121.5, 121.3, 108.3, 62.1, 53.1, 52.2, 51.9, 45.2, 41.3, 41.1, 40.0, 39.6, 39.1, 36.0, 35.3, 34.8, 30.9, 29.2, 28.7, 28.3, 27.4, 24.5, 24.0, 22.5, 21.7, 20.2, 19.8, 16.5, 13.1.
Step 1: Compound X23 (160 mg, 0.30 mmol) was treated according to general procedure D to give (S)-1-(chloromethyl)-2,3-dihydro-1H-benzo[e]indole-5-yl (cis)-hexahydro-[1,2]dithiino [4,5-b]pyridine a colourless solid.
Step 2: The material obtained in step 1 was dissolved in DMF (0.03 M). Similarly to Tercel et al. (Tercel, M. et al. Angew. Chem., Int. Ed. 2011, 50, 2606-2609. https://doi.org/10.1002/anie.201004456), X21 (133 mg, 68% w/w, 0.33 mmol), EDCl (228 mg, 0.33 mmol) and p-TSA (52 mg, 0.30 mmol) were added. The solution was stirred at r.t. for 15 h, concentrated under reduced pressure, further dried by azeotroping with n-heptane, and purified by FCC (DCM/MeOH) to afford P10 (137 mg, 0.20 mmol, 66%) as a colourless solid. Purification for in vivo application was achieved by preparative HPLC (H2O/MeCN, 0.1% FA) to afford the compound P10 as a colourless solid.
Rf=0.48 (DCM:MeOH, 5:1). HRMS (ESI+) for C36H40N4O4ClS2: calc 691.21740. found 691.21717. 1H-NMR (400 MHz, CDCl3): δ (ppm)=9.85-9.53 (m, 1H), 8.35 (s, 1H), 7.82 (t, J=9.3 Hz, 1H), 7.76 (dd, J=8.2, 4.3 Hz, 1H), 7.60-7.50 (m, 1H), 7.44 (t, J=7.3 Hz, 1H), 7.35 (dd, J=8.8, 2.9 Hz, 1H), 7.09 (d, J=2.4 Hz, 1H), 7.00 (d, J=2.0 Hz, 1H), 6.93 (d, J=9.2 Hz, 1H), 4.78 (d, J=10.8 Hz, 1H), 4.72-4.61 (m, 1H), 4.58 (d, J=12.3 Hz, 1H), 4.39 (d, J=24.8 Hz, 2H), 4.15 (d, J=9.8 Hz, 1H), 4.08 (t, J=5.6 Hz, 2H), 3.96 (d, J=11.4 Hz, 1H), 3.90-3.78 (m, 2H), 3.64 (t, J=5.4 Hz, 1H), 3.50 (t, J=11.0 Hz, 2H), 3.33 (t, J=7.5 Hz, 2H), 3.20 (d, J=7.1 Hz, 1H), 3.02-2.89 (m, 1H), 2.86-2.78 (m, 2H), 2.76-2.68 (m, 1H), 2.54 (dd, J=13.1, 3.8 Hz, 1H), 2.45-2.35 (m, 2H), 2.23 (d, J=6.9 Hz, 4H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=153.1, 141.3, 131.6, 130.5, 128.8, 128.2, 127.8, 125.9, 125.3, 122.8, 122.6, 116.7, 113.1, 111.3, 106.2, 103.6, 64.6, 63.3, 55.1, 54.3, 53.0, 52.9, 45.8, 43.2, 42.3, 42.1, 40.7, 33.8, 31.9, 29.7, 29.4, 24.8, 23.5, 22.7.
Step 1: (S)-3-(Boc)-1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indole (115.1 mg, 0.34 mmol, 97%) was prepared from X22 (148.0 mg, 0.35 mmol) similar to the procedure described above.
Step 2: The material obtained in step 1 was coupled to X9 (88.9 mg, 0.42 mmol) according to general protocol E. Purification by FCC (isohexane/EtOAc) gave X34 as a colourless, crystalline solid (162 mg, 0.30 mmol, 86%).
Rf=0.44 (isohexane:EtOAc, 3:1). HRMS (ESI+) for C26H35ClN3O3S2: calc. 552.17520. found 552.17594. 1H-NMR (500 MHz, CDCl3): δ (ppm)=8.06 (s, 1H), 7.78 (d, J=8.4 Hz, 1H), 7.70 (d, J=8.4 Hz, 1H), 7.51 (t, J=7.3 Hz, 1H), 7.38 (t, J=7.6 Hz, 1H), 4.26 (d, J=10.8 Hz, 1H), 4.13 (t, J=10.2 Hz, 1H), 4.01 (td, J=11.1, 10.1, 5.0 Hz, 2H), 3.96-3.82 (m, 2H), 3.46 (t, J=10.7 Hz, 1H), 3.21 (d, J=5.9 Hz, 2H), 3.03-2.91 (m, 1H), 2.83 (dd, J=13.5, 2.6 Hz, 1H), 2.28-2.14 (m, 1H), 2.12-1.99 (m, 1H), 1.93-1.78 (m, 1H), 1.77-1.67 (m, 1H), 1.58 (s, 9H). 13C-NMR (126 MHz, CDCl3): δ (ppm)=154.0, 153.2, 152.5, 148.4, 141.5, 131.4, 130.3, 128.1, 127.7, 124.5, 122.6, 122.5, 120.4, 109.4, 81.9, 63.8, 54.7, 47.0, 43.4, 41.2, 40.8, 39.1, 37.2, 28.5, 26.9, 23.3.
Step 1: Compound X34 (22.0 mg, 0.04 mmol) was treated according to general procedure D to give (S)-1-(chloromethyl)-2,3-dihydro-1H-benzo[e]indole-5-yl (trans)-hexahydro-[1,2]dithiino [4,5-b]pyridine a colourless solid.
Step 2: The material obtained in step 1 was dissolved in DMF (0.03 M). Similarly to Tercel et al. (Tercel, M. et al. Angew. Chem., Int. Ed. 2011, 50, 2606-2609. https://doi.org/10.1002/anie.201004456), X21 (23.2 mg, 0.05 mmol), EDCl (31 mg, 0.16 mmol) and p-TSA (7 mg, 0.04 mmol) were added. The solution was stirred at r.t. for 15 h, before being poured into a mixture of sat. aq. NaCl/H2O (1:1, 20 mL). The organic layer was separated and the aq. layer was extracted with EtOAc (3×15 mL), the combined organic layers were dried over Na2SO4, filtered, concentrated under reduced pressure and purified by FCC (isohexane/EtOAc) to yield X34 (12.6 mg, 0.02 mmol, 50%) as a colourless solid.
Rf=0.59 (isohexane:EtOAc, 1:1). HRMS (ESI+) for C39H46N4NaO6S2Cl: calc 787.23613. found 787.23632. 1H-NMR (400 MHz, CDCl3): δ (ppm)=9.47 (s, 1H), 8.40 (s, 1H), 7.85 (d, J=8.4 Hz, 1H), 7.76 (dd, J=8.2, 2.2 Hz, 1H), 7.55 (t, J=7.6 Hz, 1H), 7.49-7.43 (m, 1H), 7.36-7.27 (m, 1H), 7.11 (s, 1H), 7.05-7.00 (m, 1H), 6.97 (ddd, J=8.9, 3.8, 2.4 Hz, 1H), 4.82 (d, J=10.7 Hz, 1H), 4.67 (t, J=9.6 Hz, 1H), 4.15 (dd, J=11.4, 8.2 Hz, 1H), 4.08-3.97 (m, 4H), 3.97-3.88 (m, 2H), 3.54-3.48 (m, 1H), 3.45 (t, J=7.0 Hz, 3H), 3.22 (d, J=6.8 Hz, 2H), 3.03-2.92 (m, 1H), 2.90 (s, 3H), 2.83 (dd, J=13.4, 2.3 Hz, 1H), 2.27-2.14 (m, 1H), 2.06 (dt, J=13.6, 3.6 Hz, 3H), 1.85 (d, J=6.3 Hz, 1H), 1.78-1.68 (m, 1H), 1.64 (s, 3H), 1.45 (s, 9H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=160.6, 156.0, 154.0, 148.2, 147.0, 141.5, 131.5, 130.4, 129.9, 128.4, 127.9, 127.2, 125.4, 125.0, 123.5, 122.9, 122.7, 122.5, 122.1, 117.4, 112.9, 111.4, 106.2, 103.4, 79.5, 65.6, 63.2, 55.2, 46.2, 46.0, 43.5, 41.1, 40.7, 39.8, 37.1, 34.7, 29.8, 28.6, 28.2, 27.7, 27.3, 23.3.
X35 (5.1 mg, 0.007 mmol) was treated according to general procedure D and the residue was purified by preparative HPLC (H2O/MeCN, 0.1% FA) to afford the compound P11 as a colourless solid.
Rf=0.15 (DCM:MeOH, 5:1). 1H-NMR (600 MHz, DMSO-d6): δ (ppm)=11.65 (s, 1H), 8.49 (s, 2H), 8.21 (s, 1H), 8.04 (d, J=8.3 Hz, 1H), 7.86-7.79 (m, 1H), 7.63 (t, J=7.7 Hz, 1H), 7.57-7.51 (m, 1H), 7.42 (d, J=8.9 Hz, 1H), 7.18 (d, J=1.9 Hz, 1H), 7.17-7.10 (m, 1H), 6.95 (dd, J=8.9, 2.3 Hz, 1H), 4.88 (t, J=10.0 Hz, 1H), 4.61 (d, J=10.8 Hz, 1H), 4.42 (s, 1H), 4.13-4.06 (m, 3H), 4.00 (dd, J=11.2, 6.8 Hz, 1H), 3.30 (t, J=11.3 Hz, 1H), 3.08 (dt, J=14.5, 7.6 Hz, 3H), 3.01 (d, J=11.1 Hz, 1H), 2.95 (d, J=12.5 Hz, 1H), 2.63-2.59 (m, 3H), 2.50 (dd, J=3.9, 2.0 Hz, 2H), 2.38 (s, 1H), 2.21 (q, J=11.1 Hz, 1H), 2.08 (p, J=6.3 Hz, 2H), 1.94 (s, 1H), 1.81 (s, 1H), 1.69 (t, J=10.8 Hz, 1H), 1.45 (p, J=12.3, 10.8 Hz, 1H). 13C-NMR (151 MHz, DMSO-d6): δ (ppm)=160.2, 153.2, 152.8, 147.2, 141.5, 139.3, 131.8, 130.8, 129.5, 127.7, 127.5, 125.2, 124.3, 123.5, 122.3, 122.2, 115.9, 113.3, 110.6, 109.5, 105.5, 103.3, 67.8, 65.0, 62.5, 54.9, 47.7, 46.0, 41.2, 33.7, 32.7, 26.3, 25.6, 21.9.
Step 1: (S)-3-(Boc)-1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indole (90.6 mg, 0.27 mmol, 99%) was prepared from X22 (114.2 mg, 0.27 mmol) similar to the procedure described above.
Step 2: The material obtained in step 1 was coupled to cis-X29 (134.0 mg, 63 w %, 0.34 mmol) according to general protocol E. Purification by FCC (EtOAc) gave X36 as a colourless solid (97.1 mg, 0.18 mmol, 67%).
Rf=0.35 (EtOAc). HRMS (ESI) for C25H31ClN3O4S2: calc. 536.14390. found 536.14355. 1H-NMR (600 MHz, CDCl3) δ (ppm)=8.05 (s, 2H), 7.80-7.72 (m, 2H), 7.70 (d, J=8.4 Hz, 2H), 7.50 (t, J=7.3 Hz, 2H), 7.37 (q, J=7.3 Hz, 2H), 4.56 (d, J=11.8 Hz, 1H), 4.37 (d, J=11.6 Hz, 1H), 4.30-4.09 (m, 5H), 4.06-3.89 (m, 5H), 3.77 (td, J=12.6, 7.6 Hz, 1H), 3.66 (t, J=12.3 Hz, 1H), 3.58-3.44 (m, 4H), 3.42 (s, 1H), 3.40-3.33 (m, 1H), 3.31-3.19 (m, 3H), 3.13-2.98 (m, 6H), 1.93 (s, 2H), 1.58 (s, 18H). 13C-NMR (151 MHz, CDCl3) δ (ppm)=153.6, 153.4, 152.5, 148.3, 141.2, 130.3, 127.7, 124.5, 124.4, 122.5, 120.6, 120.4, 109.4, 81.5, 53.2, 53.1, 52.9, 52.6, 52.0, 46.4, 45.6, 45.4, 42.4, 42.2, 41.1, 40.3, 30.0, 29.8, 29.2, 29.1, 28.5.
X36 (78.0 mg, 0.145 mmol) was dissolved in MeOH (5 mM) and a solution of formaldehyde (4.96 mL, 0.88 M in MeOH, 4.36 mmol) was added at r.t., followed by addition of a solution of NaCNBH3 (91.7 mg, 0.5 M solution in MeOH, 1.45 mmol). The reaction mixture was concentrated under reduced pressure, the residue was taken into DCM (10 mL) and washed with aq. NaCl (20 mL). The aq. layer was extracted with DCM (4×10 mL), the combined organic layers were dried over Na2SO4 and concentrated under reduced pressure. Purification was achieved by FCC (isohexane/EtOAc) to give X37 (39.8 mg, 0.072 mmol, 49%) as a colourless solid. A fraction of unreacted starting material X36 (32.7 mg, 0.061 mmol, 42%) was recovered.
Rf=0.47 (isohexane:EtOAc, 1:1). HRMS (ESI) for C26H33ClN3O4S2: calc. 550.15955. found 550.15910. 1H-NMR (800 MHz, CDCl3): δ (ppm)=8.05 (s, 2H), 7.78-7.75 (m, 1H), 7.75-7.72 (m, 1H), 7.70 (d, J=8.2 Hz, 2H), 7.51 (t, J=7.4 Hz, 2H), 7.37 (q, J=7.2, 6.5 Hz, 2H), 4.57 (d, J=10.2 Hz, 1H), 4.38 (d, J=10.8 Hz, 1H), 4.30-4.20 (m, 3H), 4.19-4.07 (m, 2H), 4.09-3.96 (m, 4H), 3.96-3.89 (m, 3H), 3.57 (s, 1H), 3.55-3.45 (m, 2H), 3.45-3.26 (m, 5H), 3.10-2.93 (m, 2H), 2.69 (d, J=7.8 Hz, 1H), 2.63 (s, 1H), 2.60-2.48 (m, 3H), 2.45 (t, J=11.4 Hz, 1H), 2.32 (s, 6H), 1.57 (s, 18H). 13C-NMR (201 MHz, CDCl3): δ (ppm)=153.3, 153.2, 152.5, 148.3, 141.3, 130.3, 127.7, 124.5, 124.4, 124.1, 122.5, 120.5, 109.4, 81.5, 61.5, 61.3, 56.3, 56.0, 54.8, 53.7, 53.1, 46.4, 42.2, 41.9, 40.9, 40.2, 39.2, 38.9, 30.7, 29.8, 28.6.
Step 1: Compound X37 (28.5 mg, 0.052 mmol) was dissolved in anhydrous DCM (0.03 M) and treated dropwise with BF3·OEt2 (33 μL, 0.26 mmol). The mixture was stirred at r.t. for 30 min, was then concentrated under reduced pressure to give (S)-1-(chloromethyl)-2,3-dihydro-1H-benzo[e]indole-5-yl (cis)-hexahydro-[1,2]dithiino [4,5-b]pyrazine dihydrochloride as a colourless solid that was used without further purification.
Step 2: 3,4,5-trimethoxy-1H-indole-2-carboxylic acid (251 mg, 1.00 mmol) was dissolved in anhydrous DCM (0.066 M, 15.0 mL) and oxalyl chloride (2 M solution in DCM: 1.50 mL, 3.00 mmol) and N,N-dimethylformamide (15.5 μL, 0.20 mmol) were added subsequently. The mixture was stirred at r.t. for 1.5 h, was then concentrated under reduced pressure and co-evaporated with anhydrous toluene to give 3,4,5-trimethoxy-1H-indole-2-carbonyl chloride (268 mg, 1.00 mmol, 99%) as a brown solid that was used without further purification. The material obtained in step 1 was dissolved in anhydrous DCM (0.01 M, 5.0 mL) and treated with DIPEA (26.4 μL, 0.16 mmol) to give a clear solution. The resulting clear solution was then added dropwise to a solution of 3,4,5-trimethoxy-1H-indole-2-carbonyl chloride (27.9 mg, 0.11 mmol) in anhydrous DCM (3.0 mL) and the resulting mixture was further stirred at r.t. for 2 h. The mixture was poured into aqueous NaCl (1 M, 10 mL) and extracted with EtOAc (3×15 mL). The combined organic layers were dried over Na2SO4, filtered, concentrated under reduced pressure and purified by preparative HPLC (H2O/MeCN/0.1% formic acid) to yield P17 (15.5 mg, 0.022 mmol, 44%) as a colourless solid.
Rf=0.36 (DCM:MeOH, 20:1). HRMS (ESI) for C33H36ClN4O6S2: calc. 683.17593. found 683.17527. 1H-NMR (600 MHz, CDCl3): δ (ppm)=9.37 (s, 2H), 8.38 (s, 2H), 7.90-7.81 (m, 2H), 7.78 (d, J=8.3 Hz, 2H), 7.55 (t, J=7.5 Hz, 2H), 7.45 (t, J=7.5 Hz, 2H), 7.01 (d, J=2.1 Hz, 2H), 6.88 (s, 2H), 4.81 (d, J=10.6 Hz, 2H), 4.75-4.65 (m, 2H), 4.62 (dd, J=11.0, 3.3 Hz, 1H), 4.40 (d, J=11.6 Hz, 1H), 4.32-4.25 (m, 1H), 4.19 (t, J=8.4 Hz, 2H), 4.09 (s, 6H), 4.04 (d, J=11.7 Hz, 2H), 4.03-3.98 (m, 2H), 3.95 (s, 6H), 3.92 (s, 6H), 3.61 (t, J=13.0 Hz, 1H), 3.51 (t, J=11.0 Hz, 2H), 3.43-3.27 (m, 5H), 3.02 (dd, J=19.7, 11.7 Hz, 2H), 2.72 (dt, J=13.0, 3.1 Hz, 1H), 2.66 (s, 1H), 2.55 (dd, J=23.5, 13.2 Hz, 3H), 2.47 (t, J=11.7 Hz, 1H), 2.33 (s, 6H). 13C-NMR (151 MHz, CDCl3): δ (ppm)=160.4, 153.3, 153.3, 153.2, 153.1, 150.3, 148.1, 148.0, 148.0, 141.5, 141.5, 140.7, 139.0, 129.9, 129.9, 129.6, 129.6, 128.0, 127.9, 125.7, 125.4, 125.3, 125.2, 125.1, 123.7, 122.9, 122.8, 122.6, 122.6, 122.2, 122.2, 122.1, 122.1, 111.4, 111.3, 106.7, 97.6, 61.6, 61.4, 61.3, 61.3, 56.4, 56.2, 56.2, 56.0, 55.2, 55.1, 54.8, 54.7, 53.7, 53.6, 46.0, 43.5, 42.0, 41.9, 40.9, 40.9, 40.2, 39.1, 38.8.
4-(4-methylpiperazin-1-yl)-4-oxobutanoic acid hydrochloride (68.0 mg, 0.29 μmol) was dissolved in anhydrous 1,2-dichloroethane (0.15 M) and DIPEA (98 μL, 0.56 μmol) was added followed by trimethylacetyl chloride (35 μL, 0.29 mmol). The resulting mixture was stirred at r.t. for 2 h, before X35 (22.0 mg, 0.041 mmol) in 1,2-dichloroethane (0.1 M) was added in one portion. The mixture was heated to 70° C. for 15 h, was then concentrated under reduced pressure and taken into DCM (50 mL). The organic layer was washed with aqueous NaCl (1 M, 4×20 mL), was then dried over Na2SO4 and concentrated under reduced pressure. Purification was achieved using FCC (DCM/MeOH) to yield X38 as a colourless solid (26.0 mg, 0.036 mmol, 88%).
Rf=0.53 (DCM:MeOH, 9:1). HRMS (ESI) for C34H45ClN5O6S2: calc. 718.24943. found 718.24928. 1H-NMR (400 MHz, CDCl3): δ (ppm)=8.06 (s, 1H), 7.77 (d, J=8.5 Hz, 1H), 7.71 (d, J=8.4 Hz, 1H), 7.51 (t, J=7.2 Hz, 1H), 7.39 (t, J=7.4 Hz, 1H), 4.75 (s, 1H), 4.44 (s, 2H), 4.35-4.21 (m, 1H), 4.20-4.09 (m, 1H), 4.01 (dd, J=13.6, 8.1 Hz, 3H), 3.93 (d, J=11.9 Hz, 1H), 3.89-3.62 (m, 6H), 3.57-3.40 (m, 2H), 3.20 (d, J=14.1 Hz, 1H), 3.01 (d, J=11.0 Hz, 1H), 2.81-2.54 (m, 8H), 2.46 (s, 3H), 1.57 (s, 9H). 13C-NMR (151 MHz, CDCl3): δ (ppm)=172.1, 170.4, 154.0, 152.5, 148.0, 141.2, 130.4, 127.8, 124.6, 123.9, 122.5, 120.6, 109.4, 81.5, 54.6, 54.4, 53.1, 51.9, 46.4, 45.4, 44.6, 42.2, 42.0, 41.0, 36.0, 28.5, 28.0.
Step 1: Compound X38 (14.5 mg, 0.020 mmol) was dissolved in anhydrous DCM (0.03 M) and treated dropwise with BF3—OEt2 (13 μL, 0.10 mmol). The mixture was stirred at r.t. for 30 min, was then concentrated under reduced pressure to give (S)-1-(chloromethyl)-2,3-dihydro-1H-benzo[e]indole-5-yl (trans)-4-(4-(4-methylpiperazin-1-yl)-4-oxobutanoyl)hexahydro-[1,2]dithiino[4,5-b]pyrazine-1(2H)-carboxylate hydrochloride as a colourless solid that was used without further purification.
Step 2: 3,4,5-trimethoxy-1H-indole-2-carboxylic acid (251 mg, 1.00 mmol) was dissolved in anhydrous DCM (0.066 M, 15.0 mL) and oxalyl chloride (2 M solution in DCM: 1.50 mL, 3.00 mmol) and N,N-dimethylformamide (15.5 μL, 0.20 mmol) were added subsequently. The mixture was stirred at r.t. for 1.5 h, was then concentrated under reduced pressure and co-evaporated with anhydrous toluene to give 3,4,5-trimethoxy-1H-indole-2-carbonyl chloride (268 mg, 1.00 mmol, 99%) as a brown solid that was used without further purification. The material obtained in step 1 was dissolved in anhydrous DCM (0.01 M, 3.0 mL) and treated with DIPEA (10.3 μL, 0.06 mmol) to give a clear solution. The resulting clear solution was then added dropwise to a solution of 3,4,5-trimethoxy-1H-indole-2-carbonyl chloride (10.9 mg, 0.04 mmol) in anhydrous DCM (1.5 mL) and the resulting mixture was further stirred at r.t. for 2 h. The mixture was poured into aqueous NaCl (1 M, 5 mL) and extracted with EtOAc (3×8 mL). The combined organic layers were dried over Na2SO4 concentrated under reduced pressure and purified by preparative HPLC (H2O/MeCN/0.1% formic acid) to yield P18 (10.9 mg, 0.013 mmol, 64%) as a colourless solid.
Rf=0.36 (DCM:MeOH, 20:1). HRMS (ESI) for C41H48ClN6O8S2: calc. 851.26581. found 851.26421. 1H-NMR (400 MHz, CDCl3): δ (ppm)=9.37 (s, 1H), 8.40 (d, J=3.8 Hz, 1H), 7.89-7.82 (m, 1H), 7.78 (d, J=8.3 Hz, 1H), 7.57 (t, J=7.6 Hz, 1H), 7.51-7.43 (m, 1H), 7.01 (d, J=2.2 Hz, 1H), 6.88 (s, 1H), 4.81 (d, J=10.7 Hz, 1H), 4.68 (t, J=9.6 Hz, 1H), 4.47 (s, 1H), 4.19 (t, J=9.4 Hz, 1H), 4.09 (s, 3H), 4.07-3.96 (m, 3H), 3.95 (s, 3H), 3.92 (s, 3H), 3.90-3.82 (m, 2H), 3.64 (d, J=4.7 Hz, 2H), 3.60-3.46 (m, 4H), 3.21 (d, J=14.1 Hz, 1H), 3.04 (d, J=11.2 Hz, 1H), 2.74 (d, J=8.9 Hz, 4H), 2.45 (dd, J=144.7, 4.7 Hz, 2H), 2.39 (t, J=4.9 Hz, 2H), 2.32 (s, 3H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=172.2, 170.2, 160.5, 154.0, 150.4, 147.9, 141.5, 140.8, 139.0, 130.0, 129.6, 128.0, 125.8, 125.6, 125.1, 123.7, 122.9, 122.7, 122.3, 111.4, 106.8, 97.7, 61.6, 61.3, 56.4, 55.2, 55.1, 54.8, 52.0, 46.1, 46.0, 45.3, 43.5, 42.3, 41.8, 28.3.
SN-38 (166 mg, 0.424 mmol, 1.0 equiv.) was suspended in THF (0.05 M, 8 mL), DIPEA (147 μL, 0.848 mmol, 2.0 equiv.) and 4-nitrophenylchloroformate (94.0 mg, 0.466 mmol, 1.1 equiv.) were added. The mixture was stirred until a homogeneous solution had formed and the solvent was removed in vacuo. The yellow residue was redissolved in DMF (0.05 M, 8 mL) and directly subjected to solid cis-X29 (65 wt %, 195 mg, 0.509 mmol) premixed with additional DIPEA (239 μL, 1.41 mmol, 3.3 equiv.). The reaction mixture was stirred for 48 h and was then diluted with DCM (20 mL). The organic phase was washed with a 1:1 mixture of water and brine (four times), dried over MgSO4 and concentrated in vacuo. The crude oil was purified by FCC (DCM/MeOH), followed by preparative HPLC (MeCN/H2O/0.1% formic acid), giving P19 as a yellow solid (96.0 mg, 0.161 mmol, 38%).
Rf=0.50 (DCM:MeOH 9:1). HRMS (ESI): C29H31N4O6S2: calc. 595.1680. found 595.1683. 1H-NMR (400 MHz, CDCl3): δ (ppm)=8.23 (dd, J=9.2, 2.7 Hz, 1H), 7.83 (dd, J=8.2, 2.6 Hz, 1H), 7.64 (s, 1H), 7.57 (dd, J=9.2, 2.6 Hz, 1H), 5.75 (d, J=16.3 Hz, 1H), 5.31 (d, J=16.3 Hz, 1H), 5.26 (s, 2H), 4.55-4.36 (m, 1H), 4.17-3.98 (m, 1H), 3.70 (dt, J=38.9, 12.6 Hz, 1H), 3.54 (ddd, J=14.1, 9.7, 2.6 Hz, 1H), 3.46-3.32 (m, 1H), 3.31-2.98 (m, 6H), 2.66 (ddd, J=26.5, 13.3, 4.0 Hz, 1H), 1.89 (ddt, J=17.1, 14.1, 7.1 Hz, 2H), 1.41 (td, J=7.7, 2.7 Hz, 3H), 1.04 (t, J=7.4 Hz, 3H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=174.0, 157.7, 153.3, 150.2, 147.0, 145.2, 132.1, 127.3, 125.6, 118.5, 114.6, 112.9, 98.0, 72.8, 66.4, 52.6, 52.5, 52.0, 49.4, 45.3, 31.6, 29.0, 23.2, 14.1, 14.0, 7.8.
4-(4-methylpiperazin-1-yl)-4-oxobutanoic acid hydrochloride (83.6 mg, 0.35 mmol) was dissolved in anhydrous 1,2-dichloroethane (0.05 M) and DIPEA (120 μL, 0.70 mmol) and trimethylacetyl chloride (43 μL, 0.35 mmol) were added sequentially. The resulting mixture was stirred at r.t. for 2 h, before P19 (30.0 mg, 0.05 mmol) in 1,2-dichloroethane (0.1 M) was added in one portion. The mixture was heated to 70° C. for 15 h, was then concentrated under reduced pressure and taken into DCM (50 mL). The organic layer was washed with aqueous NaCl (1 M, 4×20 mL), was then dried over Na2SO4 and concentrated under reduced pressure. Purification was achieved using FCC (DCM/MeOH) to yield P20 as a colourless solid (31.7 mg, 0.041 mmol, 81%).
RF=0.52 (DCM:MeOH 9:1). HRMS (ESI): C38H45N6O8S2: calc. 777.2735. found 777.2736. 1H-NMR (400 MHz, CDCl3): δ (ppm)=8.22 (d, J=9.2 Hz, 1H), 7.85 (d, J=2.5 Hz, 1H), 7.64 (s, 1H), 7.58 (dd, J=9.2, 2.5 Hz, 1H), 5.73 (d, J=16.3 Hz, 1H), 5.30 (d, J=16.3 Hz, 1H), 5.25 (s, 2H), 4.76 (br s, 1H), 4.47 (m, 1H), 4.36 (m, 1H), 4.09-3.89 (m, 2H), 3.80 (m, 2H), 3.74-3.58 (m, 4H), 3.50 (dd, J=14.1, 9.4 Hz, 1H), 3.27-3.10 (m, 3H), 3.02 (d, J=12.3 Hz, 1H), 2.81-2.63 (m, 4H), 2.54 (d, J=27.5 Hz, 4H), 2.40 (s, 3H), 1.89 (ddt, J=17.0, 14.2, 7.1 Hz, 2H), 1.41 (t, J=7.6 Hz, 3H), 1.02 (t, J=7.4 Hz, 3H). 13C-NMR (101 MHz, CDCl3): δ (ppm)=173.9, 172.0, 170.2, 153.6, 150.2, 149.7, 145.3, 132.1, 127.5, 127.3, 125.5, 118.6, 114.7, 98.1, 72.8, 66.3, 54.7, 54.4, 49.4, 45.6, 44.7, 41.2, 31.6, 28.3, 27.9, 23.2, 14.1, 7.9.
Step 1: Triphosgene (17.8 mg, 0.06 mmol) was dissolved in anhydrous DCM (1.0 mL), cooled to 0° C. and pyridine (48 μL, 0.6 mmol) was added dropwise. The mixture was stirred at 0° C. for 45 min to observe a yellow precipitate and X12 (21.2 mg, 0.1 mmol) was added in one portion. The mixture was stirred at r.t. for 15 h, was then diluted with DCM (3.0 mL) and 1 M aqueous HCl (6.0 mL) was added. The mixture was stirred vigorously for 30 min, then the layers were separated, the aqueous layer was extracted with DCM (2×4 mL), the combined organic layers were dried over MgSO4 and concentrated under reduced pressure to give (cis)-hexahydro-[1,2]dithiino[4,5-b]pyridine-1(2H)-carbamoyl chloride as a brown solid that was used without further purification.
Step 2: An oven-dried flask was charged with topotecan hydrochloride hydrate (35.3 mg, 0.077 mmol) and 4-dimethylaminopyridine (2.8 mg, 0.023 mmol), the flask was filled with nitrogen gas atmosphere, the solids were suspended in anhydrous DMF (1.0 mL) and triethylamine (32 μL, 0.23 mmoL) was added. The resulting solution was cooled to 0° C. and a solution of the material obtained in step 1 in DCM (0.5 mL) was added dropwise, the resulting mixture was warmed to r.t. and was further stirred for 15 h before being concentrated under reduced pressure. Purification was achieved using reverse phase column chromatography (MeCN/H2O/0.1% formic acid) using a C18 column to yield P21 as a colourless solid (32.0 mg, 0.051 mmol, 67%).
Rf=0.46 (DCM:MeOH, 9:1). HRMS (ESI) for C31H35N4O6S2: calc. 623.19925. found 623.19928. 1H-NMR (400 MHz, DMSO-d6): δ (ppm)=8.94 (s, 1H), 8.14 (s, 1H), 8.10 (d, J=9.2 Hz, 1H), 7.65 (dd, J=13.2, 9.2 Hz, 1H), 7.33 (s, 1H), 6.53 (s, 1H), 5.43 (s, 2H), 5.31 (s, 2H), 4.69-4.30 (m, 1H), 4.23-3.87 (m, 1H), 3.82-3.69 (m, 3H), 3.69-3.59 (m, 1H), 3.53-3.17 (m, 5H), 3.13-2.94 (m, 2H), 2.85-2.75 (m, 1H), 2.41-2.24 (m, 2H), 2.21 (s, 3H), 2.19 (s, 3H), 1.94-1.80 (m, 3H), 1.74-1.61 (m, 1H), 1.61-1.49 (m, 1H), 0.89 (t, J=7.3 Hz, 3H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=172.5, 163.2, 156.8, 152.7, 152.0, 150.0, 148.1, 146.3, 145.5, 129.9, 129.6, 129.4, 128.2, 126.8, 125.7, 119.0, 96.6, 72.4, 65.3, 53.9, 53.3, 53.0, 50.5, 45.1, 41.3, 35.7, 35.3, 30.3, 29.3, 28.4, 25.2, 24.7, 22.6, 7.8.
Step 1: Triphosgene (22.3 mg, 0.075 mmol) was dissolved in anhydrous DCM (1.0 mL), cooled to 0° C. and pyridine (60 μL, 0.750 mmol) was added dropwise. The mixture was stirred at 0° C. for 45 min to observe a yellow precipitate and X9 (26.5 mg, 0.125 mmol) was added in one portion. The mixture was stirred at r.t. for 15 h, was then diluted with DCM (3.0 mL) and 1 M aqueous HCl (6.0 mL) was added. The mixture was stirred vigorously for 30 min, then the layers were separated, the aqueous layer was extracted with DCM (2×4 mL), the combined organic layers were dried over MgSO4 and concentrated under reduced pressure to give (trans)-hexahydro-[1,2]dithiino[4,5-b]pyridine-1(2H)-carbamoyl chloride as a brown solid that was used without further purification.
Step 2: An oven-dried flask was charged with topotecan hydrochloride hydrate (44.8 mg, 0.098 mmol) and 4-dimethylaminopyridine (3.6 mg, 0.023 mmol), the flask was filled with nitrogen gas atmosphere, the solids were suspended in anhydrous DMF (1.0 mL) and triethylamine (41 μL, 0.29 mmoL) was added. The resulting solution was cooled to 0° C. and a solution of the material obtained in step 1 in DCM (0.5 mL) was added dropwise, the resulting mixture was warmed to r.t. and was further stirred for 15 h before being concentrated under reduced pressure. Purification was achieved using reverse phase column chromatography (MeCN/H2O/0.1% formic acid) using a C18 column to yield P22 as a colourless solid (32.0 mg, 0.051 mmol, 53%).
Rf=0.49 (DCM:MeOH, 9:1). HRMS (ESI) for C31H36N4O6S2: calc. 623.19925. found 623.19918. 1H-NMR (400 MHz, DMSO-de): δ (ppm)=8.94 (s, 1H), 8.14 (s, 1H), 8.10 (d, J=9.2 Hz, 1H), 7.64 (d, J=9.2 Hz, 1H), 7.33 (s, 1H), 6.52 (s, 1H), 5.42 (s, 2H), 5.30 (s, 2H), 3.91-3.68 (m, 3H), 3.50 (s, 1H), 3.33-3.21 (m, 1H), 3.09 (d, J=9.6 Hz, 1H), 3.02 (d, J=11.5 Hz, 1H), 2.95-2.88 (m, 1H), 2.21 (s, 6H), 2.00-1.76 (m, 4H), 1.69 (t, J=10.6 Hz, 1H), 1.39 (ddd, J=22.3, 12.7, 9.5 Hz, 1H), 0.89 (t, J=7.3 Hz, 3H). 13C-NMR (101 MHz, DMSO-d6): δ (ppm)=173.0, 163.6, 157.3, 153.5, 152.4, 150.4, 148.6, 146.7, 145.9, 130.3, 130.0, 129.9, 128.6, 127.2, 126.1, 119.5, 97.0, 72.8, 65.7, 63.0, 53.7, 51.0, 45.6, 36.4, 30.7, 26.7, 23.4, 8.3.
This assay (see
Assay Conditions: A black 96-well plate with black bottom was charged with solutions of fluorophore-releasing reduction-triggered probes that are compounds of the invention (P1, P2, P3, P4 and P5) or that are not compounds of the invention and reveal the performance of prior art systems (X19) (80 μL; 25 μM in aq. TE buffer (pH 7.4) and 1.25% vol DMSO). Solutions of challenge species chosen from the monothiol glutathione (GSH, 0.1-50 mM) and the dithiol reductant dithiothreitol (DTT, 0.1-50 mM) or phosphine reductant tris-(2-carboxyethyl) phosphine (TCEP); (20 μL; 1 mM in aq. TE buffer (pH 7.4) were added and the reaction mixtures were incubated at 37° C. for 6 h while measuring fluorescence intensity over time [F(t)] using a BMG Labtech FLUOstar Omega platereader (λexc=355 nm, Δem=530 nm for PQ-OH-releasing probes X19, P1 and P2; Δexc=485 nm, Δem=530 nm for MF-OH-releasing probes P3 and P4.
Data Representation: Fmax is the maximum possible fluorescence increase corresponding to complete reduction-triggered cargo release, i.e. the fluorescence intensity increase from time zero to signal plateau under TCEP incubation PQ-OH-releasing probes. F(t)/Fmax(t) thus gives the timecourse measurements of fluorescence intensity, as a fraction of the maximum possible fluorescence signal. Fsat is the endpoint fluorescence intensity increase for each condition, ie. Fsat=(F(6 h)). Fsat/Fmax thus gives the assay endpoint fluorescence intensity increase, as a fraction of the maximum possible fluorescence signal. In the GSH timecourse measurements shown in
Experimental Results and Discussion: In general,
Successful activation of all compounds of the invention by dithiol reductant DTT was also shown. DTT chemically mimics the active site of intracellular redox effector proteins (e.g. thioredoxin, to which it has similar reduction potential; Cheng, Z. et al. Biochemistry 2007, 46, 7875-7885. https://doi.org/10.1021/bi700442r; Houk, J. et al. J. Am. Chem. Soc. 1987, 109, 6825-6836. https://doi.org/10.1021/ja00256a040) which is relevant to the present invention.
TCEP is a non-physiological chemical reductant that rapidly and quantitatively chemically reduces all disulfides. The kinetics of signal generation during incubation with excess TCEP are therefore indicative of the speed of cargo release expected from probes after they have been reduced, and the fluorescence intensity established with excess TCEP is the maximum obtainable fluorescence from the assay. It is seen that compounds of the invention rapidly and quantitatively release their cargo upon reductive triggering and can therefore be useful as proagents for reduction-triggered release. Non-reducing control experiments (blank) shows that there is no assay interference by fluorescence increase independent of reduction, i.e. there is no non-reduction-based cargo release occurs with the compounds of the invention.
Taken together, this data shows that compounds of the invention P1, P2, P3 and P4 may be activatable specifically by vicinal dithiol type systems (such as DTT) and can rapidly release their cargo after such activation, while these compounds of the invention will be resistant to monothiols under physiological cellular conditions as well as resistant to other spontaneous degradation processes such as hydrolysis. Hence the compounds of the invention can offer the novel capacity to be specifically activated by altered states of metabolic activity and/or by specific cellular reductase enzymes, in that they can resist nonenzymatic activation by physiological cellular thiol levels. In comparison, prior art disulfide-based proagents can offer no stability against non-enzymatic reduction by monothiol species as are present in all cells at high concentrations. Compound of the invention P5 has an unusually strong and fast response to both monothiol (GSH) and dithiol reductants (DTT) and may therefore serve as a platform for intracellular activation and release of cargos.
This assay (see
Assay conditions: A black 96-well plate with black bottom was charged with solutions of fluorophore-releasing reduction-triggered probes that are compounds of the invention (P1, P2) or that are not compounds of the invention and reveal the performance of prior art systems (X19) (50 μL; 40 μM in aq. TE buffer (pH 7.4) and 4 μL DMSO). Separately, master mixes (30 μL in aq. TE buffer (pH 7.4)) containing mixtures of the proteins human recombinant thioredoxin 1 (Trx1, 0.166-16.66 μM) or thioredoxin 2 (Trx2, 3.3 μM), or human recombinant glutaredoxin 1 (“Grx1”, 3.3 μM) or glutaredoxin 2 (Grx2, 3.3 μM) and/or oxidoreductases human recombinant thioredoxin reductase 1 (TrxR1, 0.07 μM), the thioredoxin reductase Secys498Cys mutant (TrxR1-U498C, 0.07 μM), thioredoxin reductase 2 (TrxR2, 0.07 μM) or human recombinant glutathione reductase (GR, 0.07 μM), and/or GSH (3.3 μM) were prepared. Note that TrxR1 (U498C) has decreased reducing power compared to TrxR1 due to the mutation of the key vicinal selenothiol/thiol into a vicinal dithiol, but it still reduces Trx1. The appropriate mastermix was added into the probe solution and the assay was started by addition of 8-NADPH (20 μL: 1 mM in aq. TE buffer (pH 7.4), which serves as the electron donor that reduces GR and TrxR. These mixtures are intended to mimic the biological TrxR/Trx and GR/Grx redox systems, that maintain cellular redox homeostasis and maintain glutathione redox buffer status, respectively. The reaction mixtures were incubated at 37° C. for 6 h. Timecourse measurements of fluorescence intensity were conducted. Note that in the summary table, “Trx1” indicates thioredoxin 1 that is applied in its oxidised form, which should not be capable of reduction unless enzymatically reduced by either TrxR1 or TrxR1 (U498C). Similarly Grx1 is applied in an oxidised form that can only be reduced by in situ recovered GSH, as in the GR/GSH/Grx mixture.
Data Representation: (F(t)-FNADPH(t)) is the fluorescence intensity of the test solution at time t, from which is subtracted a background fluorescence level also at time t from a solution with NADPH (which itself is fluorescent, and is present in 10-fold higher concentration than the probe) and with the same protein mixture but without the probe. Fmax(t) is the maximum possible fluorescence intensity value at time t, determined by incubation with TCEP (100 μM)) similarly as for the chemical reductant assay. (F(t)−FNADPH(t))/Fmax(t) thus gives the timecourse of fluorescence signal, as a fraction of the maximum possible fluorescence signal.
The timecourse plots show fluorescence timecourses with the NADPH/TrxR1/Trx1 system, where legend entries are as follows: “TrxR1” indicates that no Trx1 is added (NADPH/TrxR1 only), a concentration of “Trx1” indicates that the given concentration of Trx1 is included as well as NADPH/TrxR1, and “TCEP” indicates the 100 μM TCEP control. In the enzyme system summary table, enzymatic processing of the various probes is summarised as “no release” (−) indicating less than 5% cargo release in the first 2 h, or “release” (+) indicating more than 30% cargo release in the first 2 h.
Experimental Results and Discussion: In general,
In contrast, probe X19 based on a prior art disulfide trigger releases cargo when challenged by various components of the cascades. It is rapidly degraded when exposed to GR/GSH and TrxR and TrxR1(U498C), with enhanced reaction kinetics when exposed to GR/GSH/Grx and TrxR/Trx. This indicates that the prior art triggers are cleavable by GSH, Grx, Trx, and TrxR, offering no specificity for any enzyme/protein and no stability against enzymatic and non-enzymatic reductions that occur with high turnover in all cells.
Taken together, this shows that compounds of the invention can have the novel feature of performing as selective diagnostics for activity of specific oxidoreductases or its redox effector protein within their system context (e.g. thioredoxin reductase in the TrxR+Trx system), whereas prior art compounds do not allow this; and more generally, compounds of the invention may be useful for selective cargo release triggered by activity of specific enzymes or triggered by unusual redox metabolic conditions.
Assay Conditions: Cells were cultured under standard conditions (Gao, L. et al. Cell Chemical Biology 2021, 28, 1-14. https://doi.org/10.1016/j.chembiol.2020.11.007), left to adhere for 24 h, then compounds were added to 100 μM with 1% vol DMSO. Fluorescence timecourse measurements were acquired as in the chemoreductant assay, with all conditions in triplicates.
Data Representation: F(t)-F(0) is the fluorescence intensity signal increase as compared to time zero signal (fluorescence intensity immediately following compound addition, before probe activation can occur), in arbitrary units although with the same detection settings for all probes releasing the same cargo. This corresponds to reduction-triggered cargo release. “ctrf” indicates cells not treated with probes, as a negative control.
Experimental Results and Discussion:
Assay Conditions: Cells were cultured under standard conditions (Gao, L. et al. Cell Chemical Biology 2021, 28, 1-14. https://doi.org/10.1016/j.chembiol.2020.11.007), left to adhere for 24 h, then P10 was added from DMSO stock solution and adjusted to 1% vol DMSO. Cells were incubated under standard conditions for 48 h, then 0.20 volume equivalents of a solution of resazurin in PBS (0.15 mg/mL) were added and incubated for 3 hours, then fluorescence (ex 544 nm, em 590 nm) was detected on a fluorescence platereader. Cell viability (%) is calculated relative to a control experiment with no P10 added, from the fluorescence values.
Experimental Results and Discussion:
Assay Conditions and Data Representation: Immunocompetent wildtype Balb/c mice were inoculated with 4T1 Balb/c mouse breast cancer cell line orthotopically in the mammary fat pad (2×105 cells) and tumor growth was monitored by caliper measurement. The day when all tumors had reached ca. 150 mm3 was defined as Day 0; on this day the mice were randomised by tumor size into groups of minimum size 5 animals (mean tumor size ca. 200 mm3), and treated. Treatments were chosen from 0.9% NaCl (“untreated”), the vascular disrupting agent combretastatin A-4 phosphate (CA4P) at 30 mg/kg administered in 100 μL NaCl (“VDA”), compound of the invention P10 at 4 mg/kg administered in 30 μL DMSO (“P10”), or a combination regime involving treatment with VDA and then 6 hours later with P10 (“VDA+P10”). All treatments were administered intraperitoneally (i.p.). CA4P was re-applied to groups “VDA” and “VDA+SS-CBI” once at day 7; no P10 treatment was re-applied to either “P10” or “VDA+P10” groups. Tumor volumes were measured as assay readout.
Experimental Results and Discussion:
Assay Conditions: A549 cells were cultured under standard conditions (Gao, L. et al. Cell Chemical Biology 2021, 28, 1-14. https://doi.org/10.1016/j.chembiol.2020.11.007), left to adhere for 24 h, then compounds were added to 10 μM or 40 μM with 1% vol DMSO. Fluorescence timecourse measurements were acquired as in the chemoreductant assay, with all conditions in triplicates.
Experimental Results and Discussion: F(t) is the raw fluorescence intensity signal increase in arbitrary units with the same detection settings the probes releasing the same cargo.
Assay Conditions: TrxR1 knockout and reference mouse embryonic fibroblasts (MEF) were a gift by Marcus Conrad (LMU Munich) and initially isolated from conditional TrxR1 knockout mouse embryos, were immortalised by lentiviral transduction. In vitro deletion of TrxR1 was achieved by Tat-Cre induced recombination and verified by PCR and Immunoblotting for TrxR1 as described in Conrad et al. Cancer Res. 2010, 70, 22, 9505. MEF knockout (ko) and control (fl/fl) cells were cultured under standard conditions, left to adhere for 24 h, then P17 or P18 were added from DMSO stock solution and adjusted to 1% vol DMSO. Cells were incubated under standard conditions for 48 h, then 0.20 volume equivalents of a solution of resazurin in PBS (0.15 mg/mL) were added and incubated for 3 h, then fluorescence (ex 544 nm, em 590 nm) was detected on a fluorescence platereader. Cell viability (%) is calculated relative to a control experiment with no P17 or P18 added, from the fluorescence values.
Experimental Results and Discussion:
Assay Conditions: Cells (A549 cells for P19 and P20; HeLa cells for P21 and P22) were cultured under standard conditions (Gao, L. et al. Cell Chemical Biology 2021, 28, 1-14. https://doi.org/10.1016/j.chembiol.2020.11.007), left to adhere for 24 h, then P19, P20, P21 or P22 were added from DMSO stock solution and adjusted to 1% vol DMSO. Cells were incubated under standard conditions for 48 h, then 0.20 volume equivalents of a solution of resazurin in PBS (0.15 mg/mL) were added and incubated for 3 hours, then fluorescence (ex 544 nm, em 590 nm) was detected on a fluorescence platereader. Cell viability (%) was calculated relative to a control experiment (no compound) from the fluorescence values.
Experimental Results and Discussion:
| Number | Date | Country | Kind |
|---|---|---|---|
| 21163944.8 | Mar 2021 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2022/057483 | 3/22/2022 | WO |
