Claims
- 1. A DNA fragment which codes for a peptide defined by the amino acid sequence: ##STR3## said DNA fragment consisting essentially of the nucleotide sequence: ##STR4##
- 2. A recombinant DNA obtained by means of ligating a cloning vector with the DNA fragment according to claim 1, in which said DNA fragment is positioned under control of regulation sequences comprising a promotor and site of ribosomal recognition.
- 3. The recombinant DNA according to claim 2 wherein said regulation sequences further comprise secretion sequences capable of inducing the expression and secretion of the peptide encoded by said DNA fragment.
- 4. The recombinant DNA according to claim 2, in which the cloning vector is a plasmid or bacteriophage.
- 5. The recombinant DNA according to claim 4, in which the cloning vector is the pEX34A plasmid.
- 6. A host microorganism transformed with a molecule of recombinant DNA according to claim 2 wherein said microorganism is selected from the group consisting of Escherichia coli, Bacillus, yeasts and mammalian cells.
- 7. A transformed microorganism as deposited with the American Type Culture Collection as ATCC 67742.
Priority Claims (1)
Number |
Date |
Country |
Kind |
21553 A/88 |
Jul 1988 |
ITX |
|
Parent Case Info
This is a divisional of application Ser. No. 07/384,411, filed Jul. 24, 1989, now U.S. Pat. No. 5,130,415.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4223002 |
Newman |
Sep 1980 |
|
Non-Patent Literature Citations (1)
Entry |
Morinaga et al. (1983) Proc. Natl. Acad. Sci. 80, 4604-4603. |
Divisions (1)
|
Number |
Date |
Country |
Parent |
384411 |
Jul 1989 |
|