DNA encoding the 15 kD outer membrane protein of Haemophilus influenzae

Abstract

Murine monoclonal antibodies directed against a novel outer membrane protein (OMP) of Haemophilus influenzae have been isolated and characterized. The gene encoding of the outer membrane protein has also been isolated and characterized. Portions of the DNA sequence of the 15 kD OMP gene are useful as probes to diagnose the presence of Haemophilus influenzae in samples. These DNA's also make available polypeptide sequences of immunoreactive epitopes encoded within the gene, thus permitting the production of polypeptides which are useful as standards or reagents in diagnostic tests and/or as components of vaccines. Monoclonal antibodies directed against epitopes of the 15 kD OMP are also useful for diagnostic tests and as therapeutic agents for passive immunization.

Description

BACKGROUND OF THE INVENTION

1. Field of the Invention

This invention relates to the 15 kD outer membrane protein of Haemophilus influenzae type b and nontypable Haemophilus influenzae.

For the sake of simplicity, Haemophilus influenzae is hereinafter referred to as H. influenzae.

2. Discussion of the Prior Art

Haemophilus influenzae type b is a major cause of meningitis and other invasive bacterial diseases in children under the age of five. Efficacious vaccines have been produced. The vaccines contain the type b capsular polysaccharide conjugated to a carrier protein. Nontypable H. influenzae cause surface mucosal infections in children and adults. Such organisms also cause invasive disease in children in the developing world and immunocompromised patients. The vaccines which have been developed to prevent disease due to type b organisms are not effective against nontypable H. influenzae.

SUMMARY OF THE INVENTION

Outer membrane proteins elicit antibodies which are protective in animal models and therefore should be considered as components in the next generation of vaccines designed to prevent both serotype b and nontypable Haemophilus disease.

The object of the invention is to clone the gene for this H. influenzae protein, and to determine the DNA sequence thereof.

Accordingly, the present invention relates to a recombinant polynucleotide comprising a nucleotide sequence for the 15 kD protein of Haemophilus influenzae type b and nontypable Haemophilus influenzae, said protein having the amino acid sequence as follows:

10       20        30         40       50        60
GATCCCACCTTGTTTATTCCAATAATGGAACTTTATTTTATTAAAGGTATCTAAGTAGCA
70         80        90      100        110      120
CCCTATATAGGGATTAATTAACGAGGTTTAATAATGAACTTTAACTAAAATTTTACCAGC
130      140       150        160       170      180
ATTTGCTGCTGTAGTCTGTATTATCTGCTTGTGCAAAGGATGCACCTGAAATGACAAAAT
MetThrLysS
190       200       210       220       230      240
CATCTGCGCAAATAGCTGAAATGCAAACACTTCCAACAATCACTGATAAAACAGTTGTAT
erSerAlaGlnIleAlaGluMetGlnThrLeuProThrIleThrAspLysThrValValT
250       260       270       280       290      300
ATTCCTGCAATAAACAAACGGTGACTGCTGTGTATCAATTTGAAAACCAAGAACCAGTTG
yrSerCysAsnLysGlnThrValThrAlaValTyrGlnPheGluAsnGlnGluPrnValA
310       320        330       340       350      360
CTGCAATGGTAAGTGTGGGCGATGGCATTATTGCCAAAGATTTTACTCGTGATAAATCAC
laAlaMetValSerValGlyAspGlyIleIleAlaLysAspPheThrArgAspLysSerG
370       380      390        400      410       420
AAAATGACTTTACAAGTTTCGTTTCTGGGGATTATGTTTGGAATGTAGATAGTGGCTTAA
lnAsnAspPheThrSerPheValSerGlyAspTyrValTrpAsnValAspSerGlyLeuT
430       440       450       460      470       480
CGTTAGATAAATTTGATTCTGTTGTGCCTGTCAATTTAATTCAAAAAGGTAAATCTAGCG
hrLeuAspLysPheAspSerValValProValAsnLeuIleGlnLysGlyLysSerSerA
490        500       510       520       530      540
ATAATATCATCGTCAAAAATTGTGATGTAAACGTAAAAGCAACTAAAAAAGCAAATTTAT
spAsnIleIleValLysAsnCysAspValAsnValLysAlaThrLysLysAlaAsnLeu*
550        560       570       580       590      600
AATTAATCCCAAATGAGCAGCATAATTGCTGGTTATTTATCTTCCTCGAGGGGAGATTTT
oc
610        620       630       640       650      660
TTCTTGA (SEQ ID. NOS: 1 and 2)

Nucleotide Sequence Coding for a Common Outer Membrane Protein from H. influenzae and Monoclonal Antibodies

The 15 kD outer membrane protein described herein is conserved among type b and nontypable H. influenzae. Epitopes on the native protein are recognized by the murine monoclonal antibodies 6B11, 1A6, and 5E6. The epitopes are present on the surface of intact H. influenzae cells. The gene for the 15 kD protein has been cloned and the DNA sequence thereof has been determined. The gene, when expressed in an appropriate host/vector system, produces a recombinant protein which is reactive with the monoclonal antibodies. Since the protein is antigenically highly conserved, it should receive serious consideration for inclusion in a vaccine to prevent H. influenzae disease. Moreover monoclonal antibodies and DNA probes may be used as a diagnostic tool to detect the presence of H. influenzae.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a Western blot analysis of H. influenzae with monoclonal antibodies;

FIG. 2 is a partial restriction map of plasmid pRSM1255 which contains the gene for the 15 kD outer membrane protein from H. influenzae type b strain 1613;

FIG. 3 is a Western blot analysis of the expression of the recombinant 15 kD protein;

FIG. 4 is a dot blot immunoassay of extracts of E. coli strains LE392/pGD103 and LE392/pRSM1311; and

FIG. 5 is the amino acid sequence for the polynucleotide of the present invention. ( SEQ ID NOs 1 and 2 are set forth in this Figure. )

DESCRIPTION OF THE PREFERRED EMBODIMENT GENERATION OF MONOCLONAL ANTIBODIES

Monoclonal antibodies (Mabs) were obtained from two independent fusion experiments. Mab 1A6 was generated from Balb/c mice immunized with sarcosyl-insoluble proteins of nontypable H. influenzae MTL6 as described by Hamel et al [see Journal of General Microbiology (1992), 138, 161-168] and monoclonal antibodies 6B11 and 5E6, were produced from mice immunized with outer membranes extracted from nontypable H. influenzae 12085 by the lithium chloride method described by Hamel et al [see J. Med. Microbiology, (1987) 23 163-170]. Isotype analysis revealed that the 1A6, 6B11 and 5E6 hybridomas secreted immunoglobulin IG2a, IG1 and IG3 respectively.

Referring to FIG. 1 , Western Immunoblotting analysis of outer membrane preparations was performed. Outer membrane preparations were fractionated on 16% SDS-PAGE, transferred to nitrocellulose, and probed with Mab 1A6 (lane 1 ), 6B11 (lane 2 ), 5E6 (lane 3 ) and porin-specific Mab P2-18 (lane 4 ). The analysis indicated that the monoclonal antibodies were directed against a protein with an apparent mass of 15 kD. Antibody accessibility radioimmunoassay [see Proulx et al, Infection and Immunity (1991)59, 963-970] indicated that monoclonal antibodies bound to surface-exposed epitopes on both type b and nontypable H. influenzae isolates.

CONSERVATION OF 15 KD-EPITOPES

A total of 193 H. influenzae isolates were tested by dot blot immunoassay for their reactivity with monoclonal antibodies 1A6, 6B11 and 5E6.

TABLE 1
Characteristics of                                                                         H. influenzae                                                                     strains tested for
monoclonal antibody reactivities
                                 Number Reactive/
Strains                          Total Number                                                                        a
H. influenzae                                                                     serotype b:
Division: Clonal group A1                                                                        b 27/27
Clonal group A2                  59/59
Clonal group B1                  5/5
Division: Clonal group J         1/1
H. influenzae                                                                     serotype a:
Division 1                       2/2
Division 2                       2/2
H. influenzae                                                                     serotype d: 2/2
Division 1
H. influenzae                                                                     nontypable 95/95
Other gram-negative species                                                                        c 0/19
a                                                                                Reactivity of MAb 6B11. and 5E6 was tested individually by blot immunoassay.
b                                                                                The chromosomal genotypes of                                                                                     H. influenzae                                                                                 expressing serotype a, b and d capsule were previously characterized by Dr. James Musser (see J. Musser et al “Global Genetic STructure and Molecular Epidermiology of Encopsulated                                                                                     Haemophilus Influenzae                                                                                . Reviews of Infectious Diseases 12, 75-111).
c                                                                                Nineteen isolates representing 19 other gram-negative species were tested. These are listed in Table 2.

TABLE 2
Non-                                                                        H. influenzae                                                                     isolates tested
Alcaligenes odorans
Citrobacter freundii
Flavobacterium odoratum
Edwardsiella tarda
Enterobacter cloaca
Enterobacter aerogenes
Klebsiella pneumoniae
Moraxella catharrhalis
Neisseria lactamica
Neisseria perflava
Neisseria subflava
Pseudomonas aeruginaosa
Proteus vulgaris
Providencia rettgeri
Serratia marcescens
Salmonella thyphimurium
Shigella flexneri
Shigella sonnei
Xanthomonas maltophilia

MOLECULAR CLONING OF THE GENE FOR THE 15 KD OUTER MEMBRANE PROTEIN

A lambda EMBL3 genomic library of DNA from H. influenzae strain 1613 was immunologically screened with murine monoclonal antibody 6B11 as described by Munson and Grass [see Infection and Immunity (1988) 561 2235-2242]. An immunologically reactive clone was isolated by plaque purification. A liquid lysate was prepared and DNA was purified from a Promega lambda DNA kit according to the manufacturer's directions. The Haemophilus insert was identified as a SalI fragment of approximately 16 kb. DNA from the lambda clone was partially digested with Sau3A and fragments of approximately 3-6 kb were isolated by preparative agarose gel electrophoresis. The 3-6 kb fragments were ligated into the low copy number vector pGD103 [see Deich et al, Journal of Bacteriology (1988) 489- 498] which had been digested sequentially with BamHI and alkaline phosphatase. The ligation mixture was transformed into E. coli strain LE392 and the cells were plated on LB agar containing 35 μg/ml of kanamycin. Immunologically reactive colonies were identified by screening with murine Mab 6B11. Strain LE392/pRSM1255 was saved for further study. As shown in FIG. 2 plasmid pRSM1255 has an insert of approximately 3.8 kb. Strain LE392/pRSM1255 produces the full size protein as determined by Western blot (see FIG. 3 ). Membrane preparations were fractionated by SDS-PAGE, transferred to nitrocellulose and probed with murine monoclonal antibody 6B11. Lane 1 is the total membrane preparation of H. influenzae strain 1613; lane 2 is the total membrane preparation of E. coli strain LE392/pGD103 and lane 3 is the total membrane preparation of E. coli strain LE392/pRSM1255. The full size 15 Kd protein is produced by E. coli strain LE392/pRSM1255.

In order to further subclone the gene for sequencing, pRSM1255 was partially digested with Sau3A, fragments of approximately 0.5 to 1.5 kb were isolated, ligated into BamHI-treated pGD103 and transformed into E. coli strain LE392. An immunologically positive clone, designated LE392/pRSM1311 was saved for further analysis. Western blot analysis employing Mab 6B11 indicated that the full size protein was produced by this strain (data not shown). The Haemophilus insert in pRSM1311 is approximately 0.6 kb in size. Extracts of E. coli strain LE392/pRSM1311 react with all three murine Mabs (FIG. 4 ). 5 μg of cell extracts were applied to the nitrocellulose and probed with Mabs 3B11, 1A6, and 5E6.

SEQUENCE ANALYSIS

The insert was cloned into M13mp18 and M13mp19 as a SalI to EcoRI fragment and sequenced in both directions. A 369 codon open reading frame ( SEQ ID NO: 3 ) was identified encoding a protein composed of 123 amino acids ( SEQ ID NO: 2 ) and having a molecular weight of 13,460. The open reading frame is notable for a lysine at position 3 and a cysteine at position 26 suggesting that the protein is a lipoprotein with a 25 amino acid leader peptide. ( The sequence of the protein without the leader peptide is set forth in SEQ ID NO: 4 . )

DNA and protein sequence analysis were done using Gen Bank (a trademark of NIH) and EMBL (from European Molecular Biology Organization) data bases. The nucleotide ( SEQ ID NO: 1 ) and derived amino acid sequence ( SEQ ID NO: 2 ) of the 15 kD outer membrane protein of H. influenzae type b, strain 1613 is set out in Table 3.

TABLE3
MW for gene product = i3460.    Number of amino acids = 123
10       20        30         40       50        60
GATCCCACCTTGTTTATTCCAATAATGGAACTTTATTTTATTAAAGGTATCTAAGTAGCA
70       80        90        100       110      120
CCCTATATAGGGATTAATTAACGAGGTTTAATAATGAACTTTAACTAAAATTTTACCAGC
130       140       150       160       170      180
ATTTGCTGCTGTAGTCTGTATTATCTGCTTGTGCAAAGGATGCACCTGAAATGACAAAAT
Met	ThrLys	S
190       200       210       220       230      240
CATCTGCGCAAATAGCTGAAATGCAAACACTTCCAACAATCACTGATAAAACAGTTGTAT
erSerAlaGlnIleAlaGluMetGlnThrLeuProThrIleThrAspLysThrValValT
250       260       270       280       290      300
ATTCCTGCAATAAACAAACGGTGACTGCTGTGTATCAATTTGAAAACCAAGAACCAGTTG
yrSerCysAsnLysGlnThrValThrAlaValTyrGlnPheGluAsnGlnGluProValA
310       320       330       340       350      360
CTGCAATGGTAAGTGTGGGCGATGGCATTATTGCCAAAGATTTTACTCGTGATAAATCAC
laAlaMetValSerValGlyAspGlyIleI1eAlaLysAspPheThrArgAspLysSerG
370       380       390       400       410      420
AAAATGACTTTACAAGTTTCGTTTCTGGGGATTATGTTTGGAATGTAGATAGTGGCTTAA
lnAsnAspPheThrSerPheValSerGlyAspTyrValTrpAsnValAspSerGlyLeuT
430       440       450       460       470      480
CGTTAGATAAATTTGATTCTGTTGTGCCTGTCAATTTAATTCAAAAAGGTAAATCTAGCG
hrLeuAspLysPheAspSerValValProValAsnLeuIleGlnLysGlyLysSerSerA
490       500       510       520       530      540
ATAATATCATCGTCAAAAATTGTGATGTAAACGTAAAAGCAACTAAAAAAGCAAATTTAT
spAsnIleIleValLysAsnCysAspValAsnValLysAlaThrLysLysAlaAsnLeu*
550       560       570       580       590      600
AATTAATCCCAAATGACCAGCATAATTGCTGGTTATTTATCTTCCTCGAGGGGAGATTTT
oc
610       620       630       640       650      660
TTCTTGA

The sequence was found to have no significant homology to any known proteins including two previously described Haemophilus outer membrane lipoproteins of similar size.

The research described herein was supported in part by United States Public Health Service grant R01AI17572 from the National Institutes of Health.

The sequence for the polynucleotide claimed in this application is as follows:

SEQUENCE DESCRIPTION: SEQ ID NO: 1:
GATCCCACCTTGTTTATTCCAATAATGGAACTTTATTTTATTAAAGGTATCTAAGTAGCA. 60
CCCTATATAGGGATTAATTAACGAGGTTTAATAATGAACTTTAACTAAAATTTTACCAGC 120
ATTTGCTGCTGTAGTCTGTATTATCTGCTTGTGCAAAGGATGCACCTGAAATGACAAAAT 180
CATCTGCGCAAATAGCTGAAATGCAAACACTTCCAACAATCACTGATAAAACAGTTGTAT 240
ATTCCTGCAATAAACAAACGGTGACTGCTGTGTATCAATTTGAAAACCAAGAACCAGTTG 300
CTGCAATGGTAAGTGTGGGCGATGGCATTATTGCCAAAGATTTTACTCGTGATAAATCAC 360
AAAATGACTTTACAAGTTTCGTTTCTGGGGATTATGTTTGGAATGTAGATAGTGGCTTAA 420
CGTTAGATAAATTTGATTCTGTTGTGCCTGTCAATTTAATTCAAAAAGGTAAATCTAGCG 480
ATAATATCATCGTCAAAAATTGTGATGTAAACGTAAAAGCAACTAAAAAAGCAAATTTAT 540
AATTAATCCCAAATGACCAGCATAATTGCTGGTTATTTATCTTCCTCGAGGGGAGATTTT 600
TTCTTGA                          607

The amino acid sequence for the outer membrane protein of haemophilus influenzae claimed in this application is as follows:

SEQUENCE DESCRIPTION: SEQ ID NO: 2:
Met Thr Lys Ser Ser Ala Gln Ile Ala Glu Met Gln Thr Leu Pro Thr
1               5                   10                  15
Ile Thr Asp Lys Thr Val Val Tyr Ser Cys Asn Lys Gln Thr Val Thr
20                  25                  30
Ala Val Tyr Gln Phe Glu Asn Gln Glu Pro Val Ala Ala Met Val Ser
35                  40                  45
Val Gly Asp Gly Ile Ile Ala Lys Asp Phe Thr Arg Asp Lys Ser Gln
50                  55                  60
Asn Asp Phe Thr Ser Phe Val Ser Gly Asp Tyr Val Trp Asn Val Asp
65                  70                  75                  80
Ser Gly Leu Thr Leu Asp Lys Phe Asp Ser Val Val Pro Val Asn Leu
85                  90                  95
Ile Gln Lys Gly Lys Ser Ser Asp Asn Ile Ile Val Lys Asn Cys Asp
100                 105                 110
Val Asn Val Lys Ala Thr Lys Lys Ala Asn Leu
115                 120

2 607 base pairs nucleic acid single linear DNA (genomic) NO NO misc_feature 171..539 /note=“Nucleotides 171 through 539 encode the outer membrane protein of Haemophilus Influenzae of Sequence ID No. 2.” 1GATCCCACCT TGTTTATTCC AATAATGGAA CTTTATTTTA TTAAAGGTAT CTAAGTAGCA 60CCCTATATAG GGATTAATTA ACGAGGTTTA ATAATGAACT TTAACTAAAA TTTTACCAGC 120ATTTGCTGCT GTAGTCTGTA TTATCTGCTT GTGCAAAGGA TGCACCTGAA ATGACAAAAT 180CATCTGCGCA AATAGCTGAA ATGCAAACAC TTCCAACAAT CACTGATAAA ACAGTTGTAT 240ATTCCTGCAA TAAACAAACG GTGACTGCTG TGTATCAATT TGAAAACCAA GAACCAGTTG 300CTGCAATGGT AAGTGTGGGC GATGGCATTA TTGCCAAAGA TTTTACTCGT GATAAATCAC 360AAAATGACTT TACAAGTTTC GTTTCTGGGG ATTATGTTTG GAATGTAGAT AGTGGCTTAA 420CGTTAGATAA ATTTGATTCT GTTGTGCCTG TCAATTTAAT TCAAAAAGGT AAATCTAGCG 480ATAATATCAT CGTCAAAAAT TGTGATGTAA ACGTAAAAGC AACTAAAAAA GCAAATTTAT 540AATTAATCCC AAATGACCAG CATAATTGCT GGTTATTTAT CTTCCTCGAG GGGAGATTTT 600TTCTTGA 607 123 amino acids amino acid linear protein NO misc_feature 1..123 /note=“Nucleotides 171 through 539 encode the outer membrane protein of Haemophilus Influenzae of Sequence ID No. 2.” 2Met Thr Lys Ser Ser Ala Gln Ile Ala Glu Met Gln Thr Leu Pro Thr 1 5 10 15Ile Thr Asp Lys Thr Val Val Tyr Ser Cys Asn Lys Gln Thr Val Thr 20 25 30Ala Val Tyr Gln Phe Glu Asn Gln Glu Pro Val Ala Ala Met Val Ser 35 40 45Val Gly Asp Gly Ile Ile Ala Lys Asp Phe Thr Arg Asp Lys Ser Gln 50 55 60Asn Asp Phe Thr Ser Phe Val Ser Gly Asp Tyr Val Trp Asn Val Asp 65 70 75 80Ser Gly Leu Thr Leu Asp Lys Phe Asp Ser Val Val Pro Val Asn Leu 85 90 95Ile Gln Lys Gly Lys Ser Ser Asp Asn Ile Ile Val Lys Asn Cys Asp 100 105 110Val Asn Val Lys Ala Thr Lys Lys Ala Asn Leu 115 120

SEQUENCE LISTING
(1) GENERAL INFORMATION:
(iii) NUMBER OF SEQUENCES: 4
(2) INFORMATION FOR SEQ ID NO:1:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 607 base                                                               pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: linear
(ix) FEATURE:
(A) NAME/KEY: CDS
(B) LOCATION: 171..539
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:
GATCCCACCT TGTTTATTCC AATAATGGAA CTTTATTTTA TTAAAGGTAT C                                                               TAAGTAGCA     60
CCCTATATAG GGATTAATTA ACGAGGTTTA ATAATGAACT TTAACTAAAA T                                                               TTTACCAGC    120
ATTTGCTGCT GTAGTCTGTA TTATCTGCTT GTGCAAAGGA TGCACCTGAA A                                                               TG ACA       176
Met Thr
1
AAA TCA TCT GCG CAA ATA GCT GAA ATG CAA A                                                               CA CTT CCA ACA ATC ACT      224
Lys Ser Ser Ala Gln Ile Ala Glu Met Gln T                                                               hr Leu Pro Thr Ile Thr
5                                                                                10                  15
GAT AAA ACA GTT GTA TAT TCC TGC AAT AAA C                                                               AA ACG GTG ACT GCT GTG      272
Asp Lys Thr Val Val Tyr Ser Cys Asn Lys G                                                               ln Thr Val Thr Ala Val
20                                                                                25                  30
TAT CAA TTT GAA AAC CAA GAA CCA GTT GCT G                                                               CA ATG GTA AGT GTG GGC      320
Tyr Gln Phe Glu Asn Gln Glu Pro Val Ala A                                                               la Met Val Ser Val Gly
35                                                                                40                  45                  50
GAT GGC ATT ATT GCC AAA GAT TTT ACT CGT G                                                               AT AAA TCA CAA AAT GAC      368
Asp Gly Ile Ile Ala Lys Asp Phe Thr Arg A                                                               sp Lys Ser Gln Asn Asp
55                                                                                60                  65
TTT ACA AGT TTC GTT TCT GGG GAT TAT GTT T                                                               GG AAT GTA GAT AGT GGC      416
Phe Thr Ser Phe Val Ser Gly Asp Tyr Val T                                                               rp Asn Val Asp Ser Gly
70                                                                                75                  80
TTA ACG TTA GAT AAA TTT GAT TCT GTT GTG C                                                               CT GTC AAT TTA ATT CAA      464
Leu Thr Leu Asp Lys Phe Asp Ser Val Val P                                                               ro Val Asn Leu Ile Gln
85                                                                                90                  95
AAA GGT AAA TCT AGC GAT AAT ATC ATC GTC A                                                               AA AAT TGT GAT GTA AAC      512
Lys Gly Lys Ser Ser Asp Asn Ile Ile Val L                                                               ys Asn Cys Asp Val Asn
100                                                                               105                 110
GTA AAA GCA ACT AAA AAA GCA AAT TTA TAATTAAT                                                               CC CAAATGACCA            559
Val Lys Ala Thr Lys Lys Ala Asn Leu
115                                                                               120
GCATAATTGC TGGTTATTTA TCTTCCTCGA GGGGAGATTT TTTCTTGA                                                                               607
(2) INFORMATION FOR SEQ ID NO:2:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 123 amino                                                               acids
(B) TYPE: amino acid
(D) TOPOLOGY: linear
(ii) MOLECULE TYPE: protein
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:
Met Thr Lys Ser Ser Ala Gln Ile Ala Glu M                                                               et Gln Thr Leu Pro Thr
1               5                                                                                10                  15
Ile Thr Asp Lys Thr Val Val Tyr Ser Cys A                                                               sn Lys Gln Thr Val Thr
20                                                                                25                  30
Ala Val Tyr Gln Phe Glu Asn Gln Glu Pro V                                                               al Ala Ala Met Val Ser
35                                                                                40                  45
Val Gly Asp Gly Ile Ile Ala Lys Asp Phe T                                                               hr Arg Asp Lys Ser Gln
50                                                                                55                  60
Asn Asp Phe Thr Ser Phe Val Ser Gly Asp T                                                               yr Val Trp Asn Val Asp
65                                                                                70                  75                  80
Ser Gly Leu Thr Leu Asp Lys Phe Asp Ser V                                                               al Val Pro Val Asn Leu
85                                                                                90                  95
Ile Gln Lys Gly Lys Ser Ser Asp Asn Ile I                                                               le Val Lys Asn Cys Asp
100                                                                               105                 110
Val Asn Val Lys Ala Thr Lys Lys Ala Asn L                                                               eu
115                                                                               120
(2) INFORMATION FOR SEQ ID NO:3:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 369 base                                                               pairs
(B) TYPE: nucleic acid
(C) STRANDEDNESS: single
(D) TOPOLOGY: linear
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:
ATGACAAAAT CATCTGCGCA AATAGCTGAA ATGCAAACAC TTCCAACAAT C                                                               ACTGATAAA     60
ACAGTTGTAT ATTCCTGCAA TAAACAAACG GTGACTGCTG TGTATCAATT T                                                               GAAAACCAA    120
GAACCAGTTG CTGCAATGGT AAGTGTGGGC GATGGCATTA TTGCCAAAGA T                                                               TTTACTCGT    180
GATAAATCAC AAAATGACTT TACAAGTTTC GTTTCTGGGG ATTATGTTTG G                                                               AATGTAGAT    240
AGTGGCTTAA CGTTAGATAA ATTTGATTCT GTTGTGCCTG TCAATTTAAT T                                                               CAAAAAGGT    300
AAATCTAGCG ATAATATCAT CGTCAAAAAT TGTGATGTAA ACGTAAAAGC A                                                               ACTAAAAAA    360
GCAAATTTA                                                                                                                          369
(2) INFORMATION FOR SEQ ID NO:4:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 98 amino                                                               acids
(B) TYPE: amino acid
(C) STRANDEDNESS:
(D) TOPOLOGY: linear
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:
Cys Asn Lys Gln Thr Val Thr Ala Val Tyr G                                                               ln Phe Glu Asn Gln Glu
1               5                                                                                 10                  15
Pro Val Ala Ala Met Val Ser Val Gly Asp G                                                               ly Ile Ile Ala Lys Asp
20                                                                                25                  30
Phe Thr Arg Asp Lys Ser Gln Asn Asp Phe T                                                               hr Ser Phe Val Ser Gly
35                                                                                40                  45
Asp Tyr Val Trp Asn Val Asp Ser Gly Leu T                                                               hr Leu Asp Lys Phe Asp
50                                                                                55                  60
Ser Val Val Pro Val Asn Leu Ile Gln Lys G                                                               ly Lys Ser Ser Asp Asn
65                                                                                70                  75                  80
Ile Ile Val Lys Asn Cys Asp Val Asn Val L                                                               ys Ala Thr Lys Lys Ala
85                                                                                90                  95
Asn Leu

Claims

1. The recombinant polynucleotide having the sequence        10        20        30GATCCCACCTTCTTTATTCCAATAATGGAA         40       50        60CTTTATTTTATTAAAGGTATCTAAGTAGCA,1        70         80       90CCCTATATAGGGATTAATTAACGAGGTFFA        100       110      120ATAATGAACTTTAACTAAAATTTTACCAGC,1       130       140       l50ATTTGCTGCTGTAGTCTGTATTATCTGCTT       160       170       180GTGCAAAGGATGCACCTGAaATGACAAAAT,1       190        200      210CATCTGCGCAAATAGCTGAAATGCAAACACerSerAlaGlnIleAlaGluMetGlnThrL        220       230      240TTCCAACAATCACTGATAAAACAGTTGTATeuProThrIleThrAspLysThrValValT,1       250        260      270ATTCCTGCAATAAACAAACGGTGACTGCTGyrSerCysAsnLysGlnThrValThrAlaV        280       290      300TGTATCAATTTGAAAACCAAGAACCAGTTGalTyrGlnPheGluAsnGlnGluPrnValA,1       310       32O       330CTGCAATGGTAAGTGTGGGCGATGGCATTAlaAlaMetValSerValGlyAspGlyIleI        340      350       360TTGCCAAAGATTTTACTCGTGATAAATCACleAlaLysAspPheThrArgAspLysSerG,1       370       380       390AAAATGACTTTACAAGTTTCGTTTCTGGGGInAsnAspPheThrSerPheValSerGlyA        400       410      420ATTATGTTTGGAATGTAGATAGTGGCTTAAspTyrValTrpAsnValAspSerGlyLeuT,1       430       440       450CGTTAGATAAATTTGATTCTGTTGTGCCTGhrLeuAspLysPheAspSerValValProV        460       470      480TCAATTTAATTCAAAAAGGTAAATCTAGCGalAsnLeuIleGlnLysGlyLysSerSerA,1       490       500       510ATAATATCATCGTCAAAAATTGTGATGTAAspAsnIleIleValLysAsnCysAspValA        520       530      540ACGTAAAAGCAACTAAAAAAGCAAATTATTsnValLysAlaThrLysLysAlaAsnLeu*,1       550       560       570AATTAATCCCAAATGACCAGCATAATTGCToc        580       590      600GGTTATTTATCTTCCTCGAGGGGAGATTTT      610     620    630    640    650    660TTCTTGA (SEQ. ID. NO: 1) encoding a polypeptide comprising immunoreactive epitopes of the 15 kD outer membrane protein of Haemophilus influenzae.

2. A vector comprising a recombinant polynucleotide, wherein the recombinant polynucleotide is the recombinant polynucleotide of claim 1.

3. A host cell transformed with the vector of claim 2.

4. A recombinant expression system comprising a polynucleotide encoding a polypeptide comprising one or more immunoreactive epitopes of the 15 kD outer membrane protein of claim 1, wherein the polynucleotide is operably linked to a control sequence compatible with a desired host.

5. A cell transformed with a recombinant expression system, wherein the expression system is the recombinant expression system of claim 4.

6. A recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2.

7. The recombinant polynucleotide of claim 6, comprising the nucleotide sequence of SEQ ID NO:3.

8. A recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:4.

9. A recombinant vector comprising a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2.

10. The recombinant vector of claim 9, wherein the recombinant polynucleotide comprises the nucleotide sequence of SEQ ID NO:3.

11. A recombinant vector comprising a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:4.

12. A host cell transformed with a vector comprising a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2.

13. The host cell of claim 12, wherein the recombinant polynucleotide comprises the nucleotide sequence of SEQ ID NO:3.

14. A host cell transformed with a vector comprising a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:4.

15. A recombinant expression system comprising a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2 operably linked to a control sequence compatible with a desired host.

16. The recombinant expression system of claim 15, wherein the recombinant polynucleotide comprises the nucleotide sequence of SEQ ID NO:3.

17. A recombinant expression system comprising a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:4 operably linked to a control sequence compatible with a desired host.

18. A cell transformed with a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2.

19. The cell of claim 18, wherein the recombinant polynucleotide comprises the nucleotide sequence of SEQ ID NO:3.

20. A cell transformed with a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:4.

21. A method of making a recombinant vector capable of expressing a polypeptide comprising the amino acid sequence of SEQ ID NO:2, comprisinginserting a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2 into a plasmid.

22. The method of claim 21, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:3.

23. A method of making a recombinant vector capable of expressing a polypeptide comprising the amino acid sequence of SEQ ID NO:4, comprisinginserting a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:4 into a plasmid.

24. A method of making a polypeptide comprising the amino acid sequence of SEQ ID NO:2, comprisingculturing a host cell transformed with a recombinant vector comprising a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:2, whereby the host cell expresses the polypeptide, and isolating the polypeptide.

25. The method of claim 24, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:3.

26. A method of making a polypeptide comprising the amino acid sequence of SEQ ID NO:4, comprisingculturing a host cell transformed with a recombinant vector comprising a recombinant polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:4, whereby the host cell expresses the polypeptide, and isolating the polypeptide.