DNA microarray for fingerprinting and characterization of microorganisms in microbial communities

Abstract

An array of nucleic acid probes is described for identifying and/or characterizing a microorganism. Methods are also described for detecting the presence of a microorganism in a sample, as well as determining its pathotype, using the array. Methods of assessing related infection and disease in a subject using the array are also described. Methods that characterize complex microbial communities using the array are also described.

Description

TECHNICAL FIELD

The present invention relates to a DNA array plate and uses thereof, and more particularly to an array for detecting or pathotyping a microorganism and uses thereof. The present invention also relates to the use of a DNA array plate to characterize complex microbial mixtures and microbial communities. The present invention also demonstrates the use of a DNA array plate to determine the presence of antibiotic resistance genes in complex microbial mixtures and communities.

BACKGROUND OF THE INVENTION

Single species microbial products and complex microbial mixtures containing live microorganisms (consortia) are sold commercially and used by the general public and commercial biotechnology users. From a regulatory viewpoint, there are no easy methods to characterize these consortia in terms of the taxonomy and function of the microorganisms present. The presence or absence of microbial pathogens in these products is also difficult to assess. The presence of molecular and physiological mechanisms for antibiotic or metal resistance, which are of concern in terms of spreading these traits within the bacterial pathogens to which humans may be exposed, is also difficult to assess in these microbial products. The same problems are also found when analyzing microbial populations from biotechnology processes, water, air, soil or food samples. With reference to commercial microbial products characterization and quality control, the stability of microorganisms and their reproducibility between different batches or lots from the same supplier over a period of months to years is also difficult to assess with current methods.

Presently, basic microbiology methods relying on culturing methods followed by microscopic, morphological and biochemical tests are the principal approach to characterize the microorganism(s). Once the microorganisms are grown, methods such as fatty acid analysis by gas chromatography and mass spectrometry can be applied to identify the microbial species present. Additional microbiology methods can be used to study phenotypic traits of cultured microorganisms such antibiotic resistance, metal resistance, or catabolic properties. Newer molecular methods such as the Polymerase Chain Reaction (PCR) method can be used to detect certain specific genes of interest, for instance those genes coding for bacterial toxins, antibiotic resistance genes or catabolic properties (e.g. lipases, proteases, cellulases) that are suspected of being present in a given microbial sample under study. Electrophoresis methods like such as Denaturing Gradient Gel Electrophoresis (DGGE) can be used to provide genetic fingerprints and estimates of the microbial diversity present in a given consortium.

The methods are generally complex, time-consuming and applicable only to suspected pathogens or genes of specific concern in a given sample. They are also notoriously inadequate for characterizing microbial diversity since perhaps as many as 95-99% of microorganisms in complex microbial communities cannot be cultured with existing media and methods used in laboratories. Culture methods show inherent variability in their ability to grow bacteria that have been stored for weeks or months prior to use. Also, viable but nonculturable (VBNC) microorganisms cannot be grown and detected. Electrophoretic methods such as DGGE are labor-intensive and require skilled research personnel to achieve reproducible results. They also only provide a rough genetic fingerprint of microbial diversity in a consortia, and another overly labour-intensive DNA sequencing step is required to learn more about the taxonomic composition and potential presence of pathogens in complex consortia. While PCR methods can be applied to detecting specific microorganisms or genes of interest in consortia, independent PCR tests for many different microorganisms and genes can quickly become overly complicated and cost prohibitive.

It would be highly desirable to be provided with a DNA array capable of characterizing and even discriminating between multiple microorganism species in a sample, all in one assay.

SUMMARY OF THE INVENTION

One aim of the present invention is to provide a DNA array capable of characterizing and even discriminating between multiple microorganism species in a sample, all in one assay.

Another aim of the present invention is to provide a method for characterizing numerous microorganism in a same assay.

According to one aspect of the invention, a DNA microarray is provided including immobilized probes capable of providing a genetic fingerprint of a single species or of a consortia of microorganisms of interest in diverse water, soil, food, environmental and clinical samples, and recognizing specifically and simultaneously the presence therein of a plurality of gene classes such as:

• • a) Taxonomically significant genes such as 16S genes, heat shock proteins, RNA polymerase, DNA gyrase. Through a judicious selection of probes, information can be obtained on the presence or absence of diverse and similar microorganisms of environmental or human health relevance in a parallel, simultaneous fashion.
  • b) Functionally significant genes such as lipases, cellulases or proteases. As an example, a drain-cleaning microbial consortium lacking the stable presence of known lipase genes may be suspected of poor or inconsistent efficacy.
  • c) Genes of clinical interest to humans, wild animals, pets, livestock, insects, plants, biocontrol agents, such as antibiotic resistance genes,
  • d) Genes coding for known virulence factors, growth factors and toxins, to protect against inadvertent or deliberate contamination of a microbial product or process by pathogenic agents.
  • e) Any other gene of interest such as genes coding for specific proteins or macromolecules, cell components, waste products and antimicrobial agents.

According to yet another aspect of the invention, a method for providing a genetic fingerprint of a single species or of consortia of microorganisms in diverse water, soil, food, environmental and clinical samples, and recognizing specifically and simultaneously the presence therein of a plurality of gene classes, is provided comprising, a) extracting the total DNA from a microbial sample, b) labeling the sample with a detectable label and c) applying the labeled sample to a DNA microarray, wherein specific hybridization will occur with the relevant probes or oligonucleotides printed on the DNA microarray, d) reading the microarray with means appropriate to detect the detectable label (whether radioactive, non-radioactive, fluorescent, calorimetric, immunological, enzymatic, spectrophotometric or simply by visual detection with the unaided eye or through a microscope), to provide information simultaneously on all the types of probes mentioned above as to whether the sample contains or not sequences complementary to the probes printed on the DNA microarray.

According to a further aspect of the invention, there is also provided a microarray comprising thereon cpn60 probes and other useful probes such as 16S, antibiotic resistance, virulence genes, functional genes, for characterization of commercial microbial consortia. While the use of 16S is universal for species identification, its closely conserved nature leads to difficulties when closely related species are considered, such as Bacillus megaterium and Bacillus licheniformis (8% distance between the 16S gene sequences). Under those circumstances, the use of a more rapidly evolving gene such as cpn60 gives more differentiation.

According to yet a further aspect of the invention, the use of the microarrays according to the invention in the characterization of microbial communities in food microbiology, soil microbiology, water quality analysis, bio-terrorism detection, microbial air quality and similar applications, is also provided.

In accordance with the present invention, thereis also provided an array which comprises:

• • a) a substrate; and
  • b) a plurality of nucleic acid probes specifically and simultaneously recognizing the presence of a plurality of different genes, each of said probes being bound to said substrate at a discrete location; said plurality of probes comprising a first probe for detecting a first gene of a species of a microorganism and at least another probe for detecting at least one other gene of said species or of a different species of a microorganism.

Preferably, the array comprises at least two different probes specific for a single gene. The array may have a subarray containing said at least two probes at adjacent discrete locations on said substrate.

In one embodiment of the invention, the first probe as described above is specific for a virulence gene or a fragment thereof or a sequence substantially identical thereto, and the at least one other probe is specific for an antibiotic resistance gene. Alternatively, the first probe can be specific for a variant of a virulence gene or a fragment thereof or a sequence substantially identical thereto, and the at least one other probe is specific for an antibiotic resistance gene, the first probe allowing detection of different types and/or species of microorganism.

The microorganism can be a bacterium, and more particularly one of the family Enterobacteriaceae, such as E. coli.

In a further embodiment, the virulence gene encodes a polypeptide of a class of proteins selected from the group consisting of toxins, adhesion factors, secretory system proteins, capsule antigens, somatic antigens, flagellar antigens, invasins, autotransporter proteins, and aerobactin system proteins. In another embodiment of the invention, the different genes can be selected from the group consisting of Tem, Shv, oxa-1, oxa-7, pse-4, ctx-m, ant(3″)-Ia (aadA1), ant(2″)-Ia (aadB)b, aac(3)-IIa (aacC2), aac(3)-IV, aph(3′)-Ia (aphA1), aph(3′)-IIa (aphA2), tet(A), tet(B), tet(C), tet(D), tet(E), tet(Y), catI, catII, catIII, floR, dhfrI, dhfrV, dhfrVII, dhfrIX, dhfrXIII, dhfrXV, suII, suIII, integron classe 1 3′-CS, vat, vatC, vatD, vatE, vga, vgb, and vgbB.

Preferably, in one further embodiment of the invention, the plurality of nucleic acid probes are sequences selected from the group consisting of SEQ ID NO:1 to SEQ ID NO:64, or a fragment thereof, or a sequence having at least 50% identity, preferably at least 70% identity, more preferably having 80% identity and most preferably having 90% identity with said sequences.

The plurality of different genes can also be selected from the group consisting of 16S gene, genes encoding heat shock proteins, gene encoding RNA polymerase, gene encoding DNA gyrase, gene encoding a lipase, gene encoding a cellulose, gene encoding a protease, genes of clinical interest, gene encoding virulence factor, gene encoding growth factor, and gene encoding a toxin.

In a still further embodiment of the invention, the first probe is specific for a 16S gene or a fragment thereof or a sequence substantially identical thereto, and the at least one other probe is specific for cpn60 gene.

Also, in accordance with the present invention, there is provided a method of detecting the presence of a microorganism in a sample. The method comprises the steps of:

• • a) contacting an array as described above with a sample nucleic acid of said sample; and
  • b) detecting association of said sample nucleic acid to a probe on said array;
  • wherein association of said sample nucleic acid with said probe is indicative that said sample comprises a microorganism from which the nucleic acid sequence of said probe is derived.

The method may also comprise optionally a step of extraction of the sample nucleic acid from said sample prior to contacting said sample nucleic acid with said array.

The sample can be an environmental sample (such as from water, air or soil), a biological sample (such as blood, urine, amniotic fluid, feces, tissues, cells, cell cultures and biological secretions, excretions or discharge) or a food sample. Alternatively, the biological sample can be a tissue, body fluid, secretion or excretion from a subject.

In accordance with a further embodiment of the invention, there is also provided a method for determining a pathotype of a species of a microorganism in a sample, said method comprising the steps of:

• • a) contacting the array as defined previously with a sample nucleic acid of said sample; and
  • b) detecting association of said sample nucleic acid to a probe on said array;
  • wherein association of said sample nucleic acid with said probe is indicative that said sample having a pathotype from which the nucleic acid sequence of said probe is derived.

Still in accordance with the present invention, there is also provided a method for diagnosing an infection by a microorganism in a subject, said method comprising the steps of:

• • a) contacting the array as defined previously with a sample nucleic acid of said sample; and
  • b) detecting association of said sample nucleic acid to a probe on said array;
  • wherein association of said sample nucleic acid with said probe is indicative that said sample has been infected by a microorganism from which the nucleic acid sequence of said probe is derived.

According to the present invention, there is also provided a kit comprising the array as described above together with instructions for use thereof, such as uses for

• • (a) detecting the presence of a microorganism in a sample;
  • (b) determining the pathotype of a microorganism in a sample;
  • (c) diagnosing an infection by a microorganism in a subject; or
  • (d) diagnosing a condition related to infection by a microorganism, in a subject.

(e) characterizing a microbial complex sample or microbial community on a one-time basis

• • (f) following the evolution over time of a microbial complex sample or microbial community. This may include comparison between different batches of commercial products based on complex microbial samples, comparison between similar products from different suppliers and monitoring the bacterial composition of commercial products over storage time.

INDUSTRIAL APPLICABILITY

The method proposed is generally applicable to any sample requiring microbiological analysis, such as:

• • i. single microbial species, clinical samples, commercial microbial consortia and communities of microorganisms from air, water and soil;
  • ii. food, food samples, food ingredients, livestock and pet food and the raw ingredients for making such foods;
  • iii. cosmetics, medications, pharmaceutical products and the raw ingredients to make such products;
  • iv. wastewater samples, potable water, raw water, surface water, groundwater, water treatment facilities, sewage samples;
  • v. bioreactor samples;
  • vi. human and veterinary clinical samples such as fecal or urine samples, animal tissue samples, rumen or stomach samples;
  • vii. plants, seeds, roots, plant surfaces, plant transplants, horticultural samples, nutrient recycling samples, plant rhizosphere, plant rhizoplane;
  • viii. environmental surfaces;
  • ix. samples from the manufacture or production of biological products, microorganisms, insects, protozoa;
  • x. goods produced in controlled atmosphere such as medical devices or electronics components; and
  • xi. any other samples where microorganisms can be detected and sampled.

An initial application of the invention resides in assisting biotechnology companies to meet notification requirements for consortia products under the Canadian Environmental Protection Act within Environment Canada. Commercial application may also be found within contract research or quality control laboratories. This invention could also be used for detection and/or identification of biological warfare agents camouflaged as commercial products. The invention can also be applied to any type of single microbial species or complex microbial consortium or mixture, within detection limits and given the design of suitable probes for each particular consortium. Therefore, companies that specialize in the detection and identification of microorganisms may also be interested. Also, companies involved and microbiological aspects of environmental, air quality and food monitoring, whether in consulting, R&D, quality control or research are expected to show interest worldwide. Basic research laboratories throughout the world will also be interested in the present invention.

For the purpose of the present invention the following terms are defined below.

The term “probe” is used herein interchangeably with amplicon and oligonucleotide of at least 18 or more nucleotides in length and preferably of at least 70 nucleotides in length.

The term “array” used herein is interchangeably used with the expression “array plate” or “DNA chip”.

The term “specific for” when used to set a probe in relation to a gene is intended to mean that said probe recognizes only the gene for which the probe is specific. Of course, a skilled person will appreciate that probes with silent substitutions, deletions or additions may as well be used in accordance with the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1A illustrates a number of bacteria and antibiotic resistant bacteria present in commercial consortia, grown in LB media alone or in LB media containing Ampicillin, Chloramphenicol, Kanamycin, Streptomycin or Tetracycline at 25 or 50 μg/mL concentration;

FIG. 1B illustrates the direct detection of antibiotic resistance genes in genomic DNA extracted from a commercial consortium, the sequences of the probes detecting antibiotic resistance are found in Table 1;

FIG. 2 illustrates the detection results obtained on Biozyme 5000 commercial product, wherein solid yellow box represents an expected signal, the dashed red box represents samples known to cross react and dashed orange box represents a possible signal, the content being reported in Table 2;

FIGS. 3A to 3C illustrate the content (3A) printed on the microarray plates and the discriminating power of cpn60 probes between B38 B. megaterium (500 ng of DNA) (3B) and B16 B. licheniformis (500 ng of DNA) (3C);

FIGS. 4A and 4B illustrate the Key for the amplicon microarray used to illustrate the superior discriminating power of cpn60 genes between closely related species (4A) and the detection results (4B) of Amphibacillus xylanus using a combination of cpn60 and 16S probes;

FIG. 5 illustrates the detection results of Bacillus amyloliquefaciens using a combination of cpn60 and 16S probes as set out in Table 3, using the key illustrate in FIG. 4A;

FIG. 6 illustrates the detection results of Halobacillus halophilus using a combination of cpn60 and 16S probes as set out in Table 3, using the key illustrate in FIG. 4A; and

FIG. 7 illustrates the detection results of Virgibacillus pantothenticus using a combination of cpn60 and 16S probes as set out in Table 3, using the key illustrate in FIG. 4A.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

One of the concerns associated with the increasing occurrence of highly antibiotic resistant pathogenic bacteria in hospitals has been to find ways to slow the circulation of the resistance genes. In this context, increased use of microbial biotechnology products, particularly in consumer household environments, may be a concern if these products are found to contain medically significant antibiotic resistance genes. As shown in FIG. 1, commercially available microbial consortia in Canada have been found to contain high counts of antibiotic resistant bacteria.

To address these and other antibiotic resistance genes that may be present in commercial consortia, the inventors have developed antibiotic resistance gene probes for the consortium analysis microarray. The current design uses both oligonucleotides (18 to 70 bases) and amplicons as probes, to obtain the best trade-offs in sensitivity versus specicifity.

Presently, prokaryotic taxonomy is based, in part, upon sequence differences in the gene encoding 16S ribosomal RNA. This ordering makes sense for the most part and allows the discrimination of general taxonomic groups. However, within a narrow taxonomic group such as within a particular genus, 16S becomes less reliable as a taxonomic discriminator. Other genes, such as cpn60, a gene that encodes a 60 kDa chaperonin found in all bacteria, can also be used to delineate taxonomic lines due to its greater sequence diversity than 16S. A DNA microarray was printed with a combination of amplicon probes containing the sequences of 16S and cpn60 from a number of Bacillus and Bacillus-like species. The array was then hybridized with fluorescently-labelled amplicons of 16S and cpn60 amplified from different species that were represented on the microarray. The aim of the current work was to: 1) ascertain the validity of using such a dual taxonomic factor approach for discriminating between closely-related Bacillus species, and 2) determine whether the level of target discrimination required was achievable using DNA microarrays. The results confirm the complementarity that exists through the concomitant use of both taxonomic factors, and the parallel processing inherent in DNA microarrays, makes it a powerful tool to rapidly identify bacterial isolates at the species level.

The temperature at which a hybridization is carried out appears to be a major factor in achieving specificity. The 16S and cpn60 amplicons are of similar length (520-550 bp), but the 16S amplicons have a significantly higher GC content (57%) and melting temperature than the cpn60 amplicons (44%). This makes simultaneous hybridizations of the two amplicons on the same array less than optimal. However, by hybridizing at 55° C., a temperature between the optimum for each type of amplicon, signal discrimination for the cpn60 and 16S probes was obtained.

Due to the relatively small differences in sequence amongst the 16S probes printed on the array, some cross hybridization is expected. However, cross hybridizing signal should be proportional to the sequence similarity between the probe and target. The role of the 16S probes was to discriminate between different genera of bacteria, such as Halobacillus and Bacillus, while the cpn60, due to its greater variation, could discriminate at the species level.

The concept of a dual backbone microarray assay for the taxonomic discrimination of closely related bacteria was proven to work with amplicon hybridizations. Further work will examine whether this is valid with genomic DNA hybridizations.

The following tables 1 to 3 give a summary of the current status of probe development:

TABLE 1
70-mer oligonucleotide probes for commonly
encountered antibiotic resistance genes in bacteria
		Length				BLAST result,
		of			G + C	(mm =	Accession
Gene	Oligo probe (5′to 3′)	sequence	Position	Tm	content	mismatches)	number	oligo name
Gram-
negative
tem	AAA GTT CTG CTA TGT GGC GCG	70	8674-	80.4	57.1	tem(X)	AF307748	70-tem8674
	GTA TTA TCC CGT GTT GAC GCC		8605
	GGG CAA GAG CAA CTC GGT CGC
	CGC ATA C (SEQ ID NO:1)
shv	CTC AAG CGG CTG CGG GCT GGC	70	86-17	83.7	64.3	shv(X)	AF148850	70-shv86
	GTG TAC CGC CAG CGG CAG GGT
	GGC TAA CAG GGA GAT AAT ACA
	CAG GCG A (SEQ ID NO:2)
oxa-1	AAA CAA CCT TCA GTT CCT TCA	70	256-187	74.3	44.3	oxa-1	AJ238349	70-
	AAT AAT GGA GAT GCG ACA GTA							oxa(1)256
	GAG ATA TCT GTT GAT GCA CTG
	GCG CTG C (SEQ ID NO:3)
oxa-7	GTA GCG CAG GCT AAT TTA CTG	70	295-226	75.2	45.7	oxa-13, oxa-19,	X75562	70
	CTA CTT TTA CAA AGC ACG AAA					oxa-14, pse-2,	oxa(7)295
	ACA CCA TTG ACG GCT TCG GCA					oxa-10, oxa-17,
	GAG AAC T (SEQ ID NO:4)					oxa-16, oxa-7
pse-4	CGC TGA TTG CCA TTG TAA TCC	70	348-79	72.3	41.4	pse-4, pse-5,	J05162	70-
	CAA TAT TCT CCA TTT TGA GTA					carb-6, pse-1		pse(4)348
	TCA AGA ACG GAA ACA CCT ATA
	CGA GCA G (SEQ ID NO:5)
ctx-m	ATA CAG CGG CAC ACT TCC TAA	70	143-74	80.3	55.7	ctx-m-1, ctx-m-3,	X92506	70-ctx143
	CAA CAG CGT GAC GGT TGC CGT					ctx-m-28, ctx-m-,
	CGC CAT CAG CGT GAA CTG ACG					27, ctx-m-22,
	CAG TGA (SEQ ID NO:6)					ctx-m-27, ctx-
						m-15
ant-	ATG ATG TCG TCG TGC ACA ACA	70	290-221	79.2	55.7	aadA1, aadA2	X12870	70-	70-
(3“)-la	ATG GTG ACT TCT ACA GCG CGG							aadA(1)290
(andA1)	AGA ATC TCG CTC TCT CCA GGG
	GAA GCC G (SEQ ID NO:7)
ant-	CCC GAG TGA GGT GCA TGC GAG	70	1778-	79.1	55.7	aadB	M86913	70-
(2“)-la	CCT GTA GGA CTC TAT GTG CTT		1709					aadB1778
(aadB)b	TGT AGG CCA GTC CAC TGG TGG
	TAC TTC A (SEQ ID NO:8)
aac(3)-	CAC CGG TTT GGA CTC CGA GTT	70	200-131	77.7	52.3	aacC2	S68058	70-
IIa	TTC GAA TTG CCT CCG TTA TTG							aacC(2)200
(aacC2)	CCT TCC GCG TAT GCA TCG CGA
	TAT CTC C (SEQ ID NO:9)
aac(3)-	TCG ATC AGT CCA AGT GGC CCA	70	380-311	82.7	62.9	aac(3)-IV	X01385	70-
IV	TCT TCG AGG GGC CGG ACG CTA							aac3(IV)380
	CGG AAG GAG CTG TGG ACC AGC
	AGC ACA C (SEQ ID NO:10)
aph(3′)-	GGC GCA TCG GGC TTC CCA TAC	70	1310-	79.1	54.3	aphA1, aphA7,	V00359	70
Ia	AAT CGA TAG ATT GTC CCT GAT		1241			strA,		aphA(1)1310
(aphA1)	TGC CCG ACA TTA TCG CGA GCC					Tn903
	CAT T (SEQ ID NO:11)
aph(3′)-	AGT CAT AGC CGA ATA GCC TCT	70	220-151	78.9	52.9	Tn5, aphA2,	V00618	70-
Ila	CCA CCC AAG CGG CCG GAG AAC					aph(3′)		aphA(2)220
(aphA2)	CTG CGT GCA ATC CAT CTT GTT
	CAA TCA T (SEQ ID NO:12)
tet(A)	GAT GCC GAC AGC GTC GAG CGC	70	1390-	79.5	57.1	tetA	X00006	70-tetA1390
	GAC AGT GCT CAG AAT TAC GAT		1321
	CAG GGG TAT GTT GGG TTT CAC
	GTC TGG C (SEQ ID NO:13)
tet(B)	CAA AGT GGT TAG CGA TAT CTT	70	190-121	71.8	40	tetB,Tn10	V00611	70-tetB190
	CCG AAG CAA TAA ATT CAC GTA
	ATA ACG TTG GCA AGA CTG GCA
	TGA TAA G (SEQ ID NO:14)
tet(C)	GAC TGG CGA TGC TGT CGG AAT	70	130-61	80.8	58.6	pBR322, RP1,	J01749	70-tetC130
	GGA CGA TAT CCC GCA AGA GGC					tetC...
	CCG GCA GTA CCG GCA TAA CCA
	AGC CTA T (SEQ ID NO:15)
tet(D)	CAA ACG CGG CAC CCG CCA GGG	70	1770-	83.5	64.3	tetA	X65876	70-tetD1770
	ATA ACA GCA GCA CCG GTC TGC		1701
	GCC CCA GCT TAT CTG ACC ATC
	TGC CCA G (SEQ ID NO:16)
tet(E)	GTT GAG GCT GCA ACA GCT CCA	70	370-301	78	51.4	tetE	L06940	70-tetE370
	GTC GCA CCG GTA ATA CCA GCA
	ATT AAG CGT CCC AAA TAC AAC
	ACC CAC A (SEQ ID NO:17)
tet(Y)	TTA ATA AAG CCG GAA CCA CCG	70	1770-	76.5	47.1.	tetY	AF070999	70-tetY1770
	GCA TGA TTA ATC CCA AAC CAA		1701
	TCG CAT CAA GCG CGA CAA
	CAA TGA GTG C (SEQ ID NO:18)
catI	TTT ACG GTC TTT AAA AAG GCC	70	550-481	73.1	41.1	cam, Tn9, R100,	M62822	70-cat550
	GTA ATA TCC AGC TGA ACG GTC					cat,...
	TGG TTA TAG GTA CAT TGA GCA
	ACT GAC T (SEQ ID NO:19)
catII	AGC GGT AAT ATC GAG TTT GGT	70	300-231	75.6	45.7	catII	X53796	70-cat(2)
	GGT CAG GCT GAA TCC GCA TTT						300
	AAT CTG CTG ACG ATA AAG GGC
	AAA GTG T (SEQ ID NO:20)
catIII	TTT GCT TGT TAA GCT AAA ACC	70	370-301	74.4	41.4	catIII	X07848	70-cat(3)
	ACA TGG TAA ACG ATG CCG ATA						370
	AAA CTC AAA ATG CTC ACG GCG
	AAC CCA A (SEQ ID NO:21)
floR	GAC AAA GGC CGG TGC AGT TGA	70	384-315	82.3	60	floR, pp-flo	AF252855	70-floR384
	AGA CCA AGC TGC TCC CAG AGA
	CGC AAT GAC GAA AGC CGT TGC
	GCC CGC A (SEQ ID NO:22)
dhfr1	GGT TAA AGC ATC TTT AAT TGA	70	490-421	69.2	32.9	dhfrl, Tn9	X00926	70-
	TGG AAA GAT CAA TAC GTT CTC							dhfr(1)490
	ATT GTC AGA TGT AAA ACT TGA
	ACG TGT T (SEQ ID NO:23)
dhfrV	GTA CAT GGC CTC TTC GAT CGA	70	1560-	76.6	51.4	dhfrV,dhfrlb,	X12868	70-
	CGG GAA TAC TAT TAC GTT GTC		1491			dhfrXVI		dhfr(5)1560
	ATT ATG GGC CGT CCA GGC TGA
	GCG AGT A (SEQ ID NO:24)
dhfrVII	GAA CAC CCA TAG AGT CAA ATG	70	753-684	64.2	72.4	dhfrVII, dhfrXVII	X58425	70-
	TTT TCC TTC CAA CAA GGA GCC							dhfr(7)753
	ACT GAT TAT ATG TGA GCG CTT
	TAA AGA G (SEQ ID NO:25)
dhfrIX	AGC TTT GAA GTG TTT TAA ATC	70	830-761	72.5	40	dhfrlX	X57730	70-
	TTG TGG TTC ATG CCA CGG AAT							dhfr(9)830
	CTG ATT TTC AAA TCC GAT ACC
	TCC TGT C (SEQ ID NO:26)
dhfrXIII	TGG CGC GAG AGC ACC ACT GTG	70	929-860	82.1	58.6	dhfrXIII	Z50802	70-
	TGG CGG TTT GGT AAG GGC TTG							dhfr(1 3)929
	CCT ATG GAC TCA AAT GTC TTG
	CGG CCC A (SEQ ID NO:27)
dhfrXV	CTT CAG ATG ATT TAG CGC TTC	70	620-551	71.2	38.6	dhfrXV	Z83311	70-
	ATC GAT AGA TGG AAA TAC CAA							dhfr(15)620
	TAC ATT CTC ATC ACT GGA AGT
	GAA GCT T (SEQ ID NO:28)
suII	AGC GCC GGC GGG GTC TAG CCG	70	960-891	82.5	62.9	Tn2l, Integron	X12869	70-sul
	GCG GCT CTC ATC GAA GAA GGA					class		(1)960
	GTC CTC GGT GAG ATT CAG AAT					1, sulI
	GCC GAA C (SEQ ID NO:29)
suIII	TAC GCG CCT GCG CAA TGG CTG	70	420-351	82.8	61.4	RSF1010, sulII	M36657	70-sul(2)420
	CGT CTG GCG CCA GAT ACC GGC
	CTC CAT CGG AGA AAC TGT CCG
	AGG TTA T (SEQ ID NO:30)
integron	TTG GAT GCC CGA GGC ATA GAC	70	1200-	78.3	51.4	integrase, Int1	M33633	70
classe 1	TGT ACC CCA AAA AAC AGT CAT		1131					int(1)1200
3″-CS	AAC AAG CCA TGA AAA CCG CCA
	CTG CGC C (SEQ ID NO:31)
Gram
postive
vat	TTT ACC GAT AAA AGG GAA TCG	70	2822-	68.3	31.4	AF117258	70-vat2822
	GAA TCT TCA ATT TAT AAA ACC		2753
	TAC TAT AAC GAA CGA AAA CAT
	TTT GGT G (SEQ ID NO:32)
vatC	GAA CAT GTT TAT TAC CTT CTA	70	1376-	71	37.1	AF015628	70-
	TAG GGT ATA TTT CTT CTG GAT		1307				vatC1376
	TGG GGC CTT GCT GAT TTT GCC
	ATT TCA T (SEQ ID NO:33)
vatD	TTG ATC TAA TTT TGG CAT ATG	70	3022-	69.2	32.9	AF368302	70-
	TTT CTC CCA TCC ATT ACC AAA		2953				vatD3022
	TAA ATT AAA TGG ATA TGT TGA
	GCC ATC C (SEQ ID NO:34)
vatE	CGT TCT TGA TAA AGT CTA GCT	70	70-1	71.7	41.4	AY043213	70-vatE70
	CTA TGA GGA TGA GGT TAG GAT
	AGA CTG CAT TTG CGT CAG GTA
	TAG TCA T (SEQ ID NO:35)
vga	GAG CTT CAA TTG AGG AAT AAG	70	1133-	69.1	31.4	M90056	70-vga1133
	TTC ACA ATG TGA AAA TTG TTT		1064
	TAC AAT ACC TTC TTC AGG CAC
	AAT TTT T (SEQ ID NO:36)
vgb	CCA TAT GGT ATA ACC GTT TCA	70	3720-	68.8	32.9	AF117258	70-Vgb3720
	GAT AAG GGG AAA GTT TGG ATT		3651
	ACA CAA CAT AAA GCA AAT ATG
	ATA AGT T (SEQ ID NO:37)
vgbB	CTG ATG AAG TTA TAC CGT ATG	70	468-399	68.8	34.3	AF015628	70-vgbB468
	GAC CTG AAT CGG GAA TAG ACA
	AGT TAA ACT CCT CTA AAT AAA
	AAT TCA T (SEQ ID NO:38)

The present invention will be more readily understood by referring to the following examples which are given to illustrate the invention rather than to limit its scope.

EXAMPLE I

Taxonomic Identification of Microorganisms Present in a Commercial Consortium

The following experiment was conducted to establish the concept of the invention and obtain preliminary results. A DNA microarray slide (Corning Ultragaps, Corning, N.Y.) was printed with DNA sequences using conventional technique in the art for attaching on the slide a number of sequences of genes as detailed in Table 2 below.

TABLE 2
Interpretation key (probes for Biozyme 5000 in grey)
+ cont GFP	A. oryzae 18S	P. denitrificans nos Z	+ cont GFP
− cont GFP	A. oryzae pepO	P. fluorescens 16S	− cont GFP
A. globiformis 23S		R. eutropha 16S	A. Hydrophila aly
A. oxydans recA		S. cerevisiae 18S	A. salmonicida bhem1
	B. megaterium cpn 60	S. typhi dlt	A. globiformis 16S
C. jejuni gtpase	B. megaterium merR2	S. scabies 16S	A. globiformis est
C. albicans MNT1		S. elongatus 16S	A. oxydans 16S
E. coli stx2A	B. cepacia pvdA		A. niger calnexin
+ cont A. thaliana	N. winogradskyi 16S	P. denitrificans nir S	C. jejuni gtpase
	N. europa amo A		C. albicans MNT1
S. elongatus 16S	Nitrosomonas nir K		C. parvum lax
Buffer	P. denitrificans nir S	P. denitrificans nos Z	E. faecalis 16S
Buffer		R. eutropha gyrB	E. coli gus A
Buffer		S. scabies 16S	E. coli sltlle
Buffer	P. aeruginasa 16S	S. elongatus 16S	K. pneumoniae cpn 60
Buffer	P. aeruginosa toxA	+ cont A. thaliana	N. hambergensis nor B

Table 3 lists the oligonucleotides probes immobilized on the microarray prototype used to analyze a commercial consortium; the layout of the microarray found in Table 2.

TABLE 2
Sequences of the probes for Biozyme 5000
			Gen Bank			GC
Probe	Organism	Gene	Accession no.	Start	Sequence	%	Tm
A.	A. oryzae	pepO	ASNPEPA	721-770	TTTCCAGAAGGCTTGTAGACGTCGTGGCCGTCTGCTCGGACTTGG	64	84
					GGAG (SEQ ID NO:39)
B.	A. globiformis	23S	ARG23RRNAD	11-60	CACCCACAAGGGGTGTCAGGCAGGTCTCGGGCGGTTAGTATCCCC	62	83
					TGTTC (SEQ ID NO:40)
C.	A. oxydans	recA	AF214789	1-50	TCCCAAAGCAACATCCAGGGCAATGGATCCGGTGGGGATGACCTC	58	82
					GATCG (SEQ ID NO:41)
D.	B. subtilis	16S-23S	BSUB0005	144980-	GMCACGTTTCGMGGAATGATCCTTCAAAACTAAACAAGACAGGGA	42	74
				145029	ACG (SEQ ID NO:42)
E.	A. salmonicida	bhem1	AS17BHEM1	1490-	AGTCTCGTCACAGGTCACGGCGCTCAGGCCATGCTCGGCGCCGGC	70	80
				1539	GCTCA (SEQ ID NO:43)
F.	s. typhi	dlt	STDLT	661-710	GAAGGCGGCATTGTTGATATGGTAACGGCCACGGACATACACGGA	56	74
					AGGCG (SEQ ID NO:44)
G.	P. denitrificans	nosZ	AF016059	1046-	TTCTCCGGGTGCAGCGGGCCGGTGGGCAGGAAGCGGTCCTTGGAG	66	86
				1095	AACTT (SEQ ID NO:45)
H.	P. fluorescens	16S	PSEIAM12	48-97	CCGTCCGCCTCTCTCAAGAGAAGCAAGCTTCTCTCTACCGCTCGA	58	81
					CTTGC (SEQ ID NO:46)
I.	R. eutropha	16S	AFARGSSA	146-195	CGCTTTCACCCTCAGGTCGTATGCGGTATTAGCTAATCTTTCGAC	46	70
					TAGTT (SEQ ID NO:47)
J.	A. globiformis	16S	AGRDNA16	66-115	GGGCAGGTTACTCACGTGTTACTCACCCGTTCGCCACTAATCCCC	60	82
					GGTGC (SEQ ID NO:48)
K.	S. scabies	16S	AB026210	67-116	CGTGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCA	54	74
					TCGTT (SEQ ID NO:49)
L.	A. globiformis	esterase	E04386	51-100	AGGCCGCGAGCTGGGCTGAATATTCCCGGTCTTCGCTCAGGAAAC	62	84
					GGCCA (SEQ ID NO:50)
M.	N. winogradskyi	16S	NITRGDW	46-95	ACGCGTTACTCACCCGTCTGCCACTGACGTATTGCTACGCCCGTT	58	82
					CGACT (SEQ ID NO:51)
N.	P. polymyxa	16S	AJ223989	66-115	TTACTCACCCGTCCGCCGCTAGGCTTATATAGAAGCAAGCTTCTA	48	71
					CGATA (SEQ ID NO:52)
O.	P. polymyxa	16S	AJ223989	66-115	TTACTCACCCGTCCGCCGCTAGGCTTATATAGAAGCAAGCTTCTA	48	71
					CGATA (SEQ ID NO:53)
P.	S. elongatus	16S	AF410931	206-255	TGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTG	60	84
					CCTCC (SEQ ID NO:54)
Q.	S. elongatus	16S	AF410931	206-255	TGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTG	60	84
					CCTCC (SEQ ID NO:55)
R.	Eubacterial	16S	ECRRNBZ	325-374	TTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCG	56	81
					TGTCT (SEQ ID NO:56)
S.	P. aeruginosa	16S	AB037563	52-101	TCACCCGTCCGCCGCTGAATCCAGGAGCAAGCTCCCTTCATCCGC	64	54
					TCGAC (SEQ ID NO:57)
T.	P. aeruginosa	toxA	AF227421	121-170	GAAGGTGCCGTGGTAGCCGACGAACACATAGCCGCGCTCCTCCAG	64	84
					TTGGC (SEQ ID NO:58)
U.	R.eutropha	gyrB	A6014982	51-100	CTGTGGATGGTGACCTGGATCTCGGTGCAGTAGCCGGCCAGCGCT	64	84
					TCGTC (SEQ ID NO:59)
V.	P. denitrificans	nosZ	AF016059	860-909	TCTTCCAGGTTCCATTTGACCAGCTGGCTGTCGATGAACAGCGTG	52	73
					GTGTA (SEQ ID NO:60)
W.	N. hamburgensis	norB	NHNORB	821-870	CCAGTTGAAGTAGGTCTTCTTGTACGGGCAGCCGGAGACGCACAT	60	82
					GCGCC (SEQ ID NO:61)
X.	C. albicans	MNT	CAMNT1PRT	121-170	CCAGCAGCAACATTACCGGTCTGTTTTTCATGAGCGGCGGGTGAT	50	80
					TGTGT (SEQ ID NO:62)
Y.	Negative control	GFP.	AEVGFP	595-644	GGCCTAGAGGGTCCTGTTCGCAGGTGATAAAAGGATGAGGGAAAT	52	73
	1				GTCGT (SEQ ID NO:63)
Z.	Negative control	GFP.	AEVGFP	371-420	ACACACCTAACTAGTAAACGTTTAATTTCAATCTTTTGCACATCA	30	64
	2				TAGTT (SEQ ID NO:64)

The DNA microarray slide was then hybridized overnight at 42° C. for 16 hours with 500 ng of Biozyme 5000 (Mirus B (6 Sep. 2002)) DNA. The DNA had previously been labeled with Cy3 16% in DIG hyb buffer: The hybridization volume was 6 μl on a cover slip of dimension 11 mm×11 mm. After hybridization, the cover slip is removed in 1×SC at room temperature followed by three washes. The first wash is made in 1xSSC, 0.2% SDS at 37° C. for 10 minutes. The second wash is made in 0.1xSSC, 0.2% SDS at room temperature for 5 minutes. Finally the third wash is made in 0.1xSSC, at room temperature for 5 minutes.

As a result, the interpretation key for the triplicate probes (see Table 2) identifies which spots represent which genes. Probes for any of the three bacterial species claimed to be present in the Biozyme 5000 consortia (B. subtilis, B. licheniformis, and P. polymyxa)are highlighted in grey. The strong signals were obtained from the expected microorganism Bacillus subtilis (see FIG. 2).

EXAMPLE II

Discriminating Power of cpn60 Probes Between Two Bacilleaceae Species

A microarray plate as in example I above with the same array layout and probe sequences is being used herein to illustration the superior specificity of cpn60 probes compared to 16S probes. The left panel (FIG. 3A) shows fluorescent labelled DNA from B. megaterium applied to array. The right panel (FIG. 3B) shows fluorescently labelled DNA from B licheniformis applied to array. The results obtained are illustrated in FIGS. 3A and 3B. As can be seen in FIGS. 3A and 3B, the cpn60 probe specific for B. licheniformis gives a signal when hybridized with B. licheniformis genomic DNA, but not at all with B. megaterium genomic DNA and vice versa (upper panels). This is not the case with the 16S probes (lower panels) that seem to light up much more easily and cross react with other 16S probes for different species. This results demonstrates the extra resolving power of cpn60 probes

EXAMPLE III

Microarray Using 16S and cpn60 Amplicons

A microarray plate was printed with the following sequences found in Table 3 using the key found in FIG. 4A.

TABLE 3
SEQUENCES USED FOR AMPLICON ARRAY
		GenBank
Organism	Gene	Accession no.	Sequence
subtilis	16S	ATCC 9799	TGTTAGGGAAGAACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCT	+TL,64
			AACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAACGGC
			TCGCAGGCGCTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGCGG
			AACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACA
			CCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAOCGTGGGGAGCGAACAGGAT
			TAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCT
			GCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGG
			GGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCACGTCTTGACAT
			CCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCA
			GCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCA
			GTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCC
			CCTTATGACCTGGGCTACACACGTGCTACAATGGGCAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCC
			AATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGT
			AATCGCGGATCAGATGCCGGGTGATACGTTCCCGGGCCTTGTACACCGCCCGTCACACCACGAGAGTTTG
			TAACACCCGAAGTCGGTGAGGTAACCTTTTTGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGTTC
			(SEQ ID NO: 65)
Bacillus	16S	DSM 13	AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACCGACG
licheniformis			GGAGCTTGCTCCCTTAGGTCAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGG
			ATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGATTGAACCGCATGGTTCAATCATAAAAGGTGG
			CTTTTAGCTACCACTTACAGATGGACCCGCCGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGG
			CGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTAC
			GGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGA
			AGGTTTTCGGATCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGA
			CGGTACCTAACCAGAAAGCCACGCCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGT
			TGTCCCGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTC
			AACCGGGGAGGGTCATTGGAAACTCGGGAACTTGAGTGCAGAAGAGGACAGTGGAATTCCACGTGTAGC
			CGTGAAATGCGTAGAGATGTCGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTG
			AGGCGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCT
			AAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGG
			TCGCAAGACTGAAACTCAAAGGAATTGACGCGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA
			TAGAGATAGGGCTTCCCCTTCGGGCGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTG
			AGATGTTGCGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTC
			TAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACC
			TGGGCTACACACGTGCTACAATGGGCAGAACAAAGGGCAGCGAAGCCGCGAGGCTAAGCCAATCCCACA
			AATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGG
			ATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTCTACACACCGCCCGTCACACCACGAGAGTTTGTA
			ACACCCGAAGTCGGTGAGGTAACCTTTTGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAG
			TCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCT (SEQ ID NO:66)
Bacillus	16S	NCDO	GAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGAA
pumilus		1766	GGGAGCTTGCTCCCGGATGTTAGCGGCGGACGGGTGAGTAACNCGTGGGTAACCTNCCTGTNAGACTGG
			GATAACTCCGGGAAACCGGAGCTAATACCGGATAGTTCCTTGAACCGCATNGTACAAGGATGAAAGACC
			GTNTCGGCTATCACTTACAGATNGACCCGCGGCCCATTAGCTAGTTGGTGGGGTAATGGCTCACCAAGG
			CGACGATGCGTAGCCGACCTGAGAGGGTNATCGGCCACACTGGGACTGAGACACGGCCNNGACTCCTAC
			GGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGA
			AGGTTTTCGGATCGTNAAGCTCTGTTGTTAGGGAAGAACAAGTGCGAGAGTAACTNCTCGCACCTTGAC
			GGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTNATACGTAGGTGGCAAGCGTT
			GTCCGGAATTATTGGGCGTNAAGGGCTCGCAGGCGGTTTCTTAAGTCTNATGTGAAAGCCCNCNGCTCA
			ACCGGGGAGGGTCATTGGAAACTGGGNAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCG
			GTNAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTNGTCTGTAACTNACGCTGA
			GGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTN
			AGTGTTAGGGGGTTTCCGCCCCTTAGTGCTNCANCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGT
			CGCAAGACTNAAACTCAAAGGAATTGACGGGGGCCNGCACAAGCGGTGGAGCATGTNGTTTAATTCGAA
			GNAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGNTNTCTTCGGGGA
			CAGAGTGACAGGTGGNGCATNGTNGTCGTCAGCTCGTGTCGTGAGATGTTGGOTTAAGTCCCGCAACGA
			GCGCAACCCTTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGG
			AGGAAGGTNGGGATGACGTCAAATCATCATGCCCCTTATGACCTNGGCTACACACGTGCTACAATGGAC
			AGACNAAGGGCTGCGAGACCGCAAGGTTTAGCCAATCCCATAAATCTGTTCTCAGTTCGGATCGCAGTC
			TGCNACTNGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCGCATOCCGCGGTGAATACGTTC
			CCGGGCCTNGTACACACCGCCCGTCACACCACGAGAGTTTGNAACACCC (SEQ ID NO:67)
B. amylolique-	16S	ATCC	GAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGAT
faciens		23350	GGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGG
			GATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGTTTGAACCGCATGGTTCAACATAAAGGTGGC
			TTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCG
			ACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGG
			GAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAG
			GTTTTCGGATCGTAAAGCTNTGTTGTTAGGGAAGAACAAGTGCCGTTCAAATAGGGCGGNACCTNGACG
			GTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTNATACGTAGGTGGCNAGCGTTG
			TCCGGAATTNTTGGGCGTNAAGGGCTCGCAGGCGGTTTCTTNAGTCTGATGTGAAAGCCCCCGGCTCAA
			CCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGG
			TGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTTGTAACTGACGCGAGGA
			GCGAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTG
			TTAGGGGGTTTCCGCCCCTTAGTGCTGCAGTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAA
			GACTNAAACTCAAAGGAATTGACGGGGCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGC
			GAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCTTCGGGGGCAGAGTGA
			ACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAAC
			CCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGG
			TGGGGATGACGTCAAATCATCATGCCCCTTATGACCTNGGCTACACACGTGCTACNATGGGCAGAACNA
			AGGGCAGCGAAACCGCGAGGTCAAGCCAATCCCACAAATCTATTCTCAGTTCGGATCGCAGTCTGCAAC
			TCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCC
			TTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCC (SEQ ID NO:68)
Bacillus	16S	IAM	GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGGATTAAGAGCTTGCTCTTATGAAG
cereus		12605	TTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGG
			GGCTAATACCGGATAACATTTTGAACCGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATG
			GATGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGGCTCACCAAGGCAACGATGCGTAGCCGAC
			CTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGA
			ATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAA
			ACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAG
			CACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCG
			TAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGG
			AAACTGGGAGACTTGAGTGCAGAAGAGGAAGTGGATTCCATGTGTAGCGGTGAAATGCGTAGAGATATG
			GAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGC
			AAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCC
			CTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAG
			GAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC
			AGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTGACAGGTGGTGC
			ATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTA
			GTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAGGTGGGGATGACGT
			CAAATCATCAGTGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTGCAAG
			ACCGCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTGTAGCTGCAACTCGCCTACATGA
			AGCTGGATCGCTAGTAATCGCGGATCAGATGCCGCGGTGATACGTTCCCGGCCTTGTACACACCGCCCG
			TCACACCACGAGAGTTTGTAACACCCGAGTCGGTGGGGTAACCTTTTTGGAGCCAGCCGCCTAAGGTGG
			GACAGATGATTGGGGTGAAGTCGTAACAA (SEQ ID NO:69)
Bacillus	16S	NUB3621	GCGGCGTGCCTAATACATGCAGTCGAGCGGACTCGCGGCGAGCTTGCTTTGCCTTGGTCAGCGGCGGAC
stearothermo-			GGGTGAGTAACACGTGGGTAACCTGCCCGCAAGACCGGGATAACTCCGGGAACCGGGGCTAATACCGGA
philus			TAACACCGAGACCGCATGGTCTTCGGTTGAAAGGCGGCTTCGGCTGCCACTTACTGATGGGCCCGCGGC
			GCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGGCCTGAGAGGGTGACCG
			CACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGAATCTTCCCAATGGACGAA
			AGTCTGACGGAGCGACGCCGCGTGAGCGAAGAAGGCCTTCGGGACGTAAAGCTCTGTTGTTAGGGAAGA
			AGAAGTGCCGTTCGAACAGGGCGGTCCGGTGAACGTACCTACCGAGAAAGCCCCGGCTAACTACGTGCC
			AGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGG
			TCCCTTAAGTCTGATGTGAAAGCCCACGGCTTAACCGTGGAGGGTCATTGGAAACTGGGGGACTTGAGT
			GCAGGAGAGGAGACGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCG
			AAGGCGGCTCTCTGGTCCGTCTCTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACC
			CTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGGTATTCCCTTTAGTGCTGTATCTAA
			CGCGTTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGC
			ACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCCTG
			ACAACCCTGGAGACAGGGCGTTCCCCCCTTGCGGGGACAGGGTGACAGGTGGTGCATGGTTGTCGTCAG
			CTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAAGCGCAACCCTCGCCCCTAGTTGCCAGCATTCATTT
			GGGCACTCTAGGGGGACTGCCGGCTAAAACTCAGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCC
			TTATGACCTGGGCTACACACGTGCTACAATGGGCGGTACAAAGGGCTGCGAACCCGCGAGGGGGAGCGA
			ATCCCAAAAAGCCGCTCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGT
			AATCGCGATCAGCATGCCCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAG
			CTTGCAACACCC (SEQ ID NO:70)
Bacillus	16S	IAM	GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACTGATTAGAAGCTTGCTTCTATGACG
megaterium		13418	TTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGA
			GGCTAATACCGGATAGGATCTTCTCCTTCATGGGAGATGATTGAAAGATGGTTTCGGCTATCACTTACA
			GATGGCCCGGGTGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCACGATGCGTAGCCGACCTGA
			GAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCT
			TCCGCAATGGACGAAACTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTC
			TGTTGTTAGGGAAGAACAAGTACAAGAGTAACTGCTTGTACCTTGACGGTACCTAACCAGAAAGCCACG
			GCTAACTACGTGCCAGCAGCCGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTT
			GAGTGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAG
			TGGCGAAGGCGGCTTTTTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAG
			ATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGC
			AGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGG
			GCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACAT
			CCTCTGACAACTCTAGAGATAGAGCGTTCCCCTTCGGGGGACAGAGTGACAGGTGGTGCATGGTTGTCG
			TCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCA
			TTTAGTTGGTGCACTTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATC
			ATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAGGGCTGCAAGACCGCGAGGTC
			AAGCCAATCCCATAAAACCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATC
			GCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACAC
			CACGAGAGTTTGTAACACCCGAAGTCGGTGGAGTAACCGTAAGGACGTAGCCGCCTAAGGTGOGACAGA
			TGATTGGGGTGAAGTCGTAACAA (SEQ ID NO:71)
Bacillus	16S	IAM	GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGTGCGGACCTTTTAAAAGCTTGCTTTTAAAAGG
coagulans		12463	TTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACNGGGATAACGCCGGGAAACCG
			GGGCTAATACCNGATAGTTTTTTCCTCCGCATGGAGGAAAAAGGAAAGGCGGCTTCGGCTGCCACTTAC
			AGATGGGCCCGCGGCGCATTAGCTAGTTGGCGGGGTAACGGCCCACCAAGGCAACGATGCGTAGCCGAC
			CTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGA
			ATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGAAGAAGGCCTTCGGGTCGTAAA
			ACTCTGTTGCCGGGGAAGAACAAGTGCCGTTCGAACAGGGCGGCGCCTTGACGGTACCCGGCCAGAAAG
			CCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGC
			GTAAAGCGCGCGCAGGCGGCTTCTTAAGTCTGATGTGAAATCTTGCGGCTCAACCAAGCGGTCATTGGA
			AACTGGGAGGCTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGT
			GGAGGAACACCAGTGGCGAAGGCGGCTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAG
			CAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGC
			CCTTTAGTGCTGCACTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAG
			GAATTGACGGGGGCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA
			GGTCTTGACATCCTCTGACCTCCCTGGAGACAGGGCCTTCTTCGGGGGACAGAGTGACAGGTGGTGCAT
			GGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGACCTTAGT
			TGCCAGCATTGAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTC
			AAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGGGCTGCGAGAC
			CGCGAGGTTAAGCCAATCCCAGAAAACCATTCCCAGTTCGGATTGCAGGCTGCAACCCGCCTGCATGAA
			GCCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGC
			CCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTANGGAGCCAGCCGCCGAAG
			GTGGGACAGATGATTGGGGTGAAGTCGTAACAA (SEQ ID NO:72)
Alicycloba-	16S	DSM 446	CCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACTTTTCGGAGGTCAGC
cillus			GGCGGACGGGTGAGGAACACGTGGGTAATCTGCCTTTCAGACCGGAATAACGCCCGGAAACGGGCGCTA
acidocaldarius			ATGCCGGATACGCCCGCGAGGAGGCATCTTCTTGCGGGGAAAGGCCCGATTGGGCCGCTGAGAGAGGAG
			CCCGCGGCGCATTAGCTGGTTGGCGGGGTAACGGCCCACCAAGGCGACGATGCGTAGCCGACCTGAGAG
			GGTGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCC
			GCAATGGGCGCAAGCCTGACGGAAGCAACGCCGCGTGAGCGAAGAAGGCCTTCGGGTTGTAAAGCTCTG
			TTGCTCGGGGAGAGCGGCATGGGGAGTGGAAAGCCCCATGCGAGACG
			GTACCGAGTGAGGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTAGGGGGCGAGCGTTG
			TCCGGAATCACTGGGCGTAAAGGGTGCGTAGGCGGTCGAGCAAGTCTGGAGTGAAAGTCCATGGCTCAA
			CCATGGGATGGCTCTGGAAACTGCTTGACTTCAGTGCTGGAGAGGCAAGGGGAATTCCACGTGTAGCGG
			TGAAATGCGTAGAGATGTGGAGGAATACCTGTGGCGAAGGCGCCTTGCTGGACAGTGACTGACGCTGAG
			GCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAG
			GTGTTGGGGGGACACACCCCAGTGCCGAAGGAAACCCAATAAGCACTCCGCCTGGGGAGTACGGTCGCA
			AGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGCATGTGGTTTAATTCGAAGCAA
			CGCGAAGAACCTTACCAGGGCTTGACATCCCTCTGACCGGTGCAGAGATGCACCTTCCCTTCGGGGCAG
			AGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTCAGTCCCGCAACGAGC
			GCAACCCTTGACCTGTGTTACCAGCGCGTTGAGGCGGGGACTCACAGGTGACTGCCGGCGTAAGTCGGA
			GGAAGGCGGGGATGACGTCAAATCATCATGCCCCTGATGTCCTGGGCTACACACGTGCTACAATGGGCG
			GTACAAAGGGAGGCGAAGCCGCGAGGCGGAGCGAAACCCAAAAAGCCGCTCGTAGTTCGGATTGCAGGC
			TGCAACTCGCCTGCATGAAGCCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCC
			CGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTCGOCAACACCCGAAGTCGGTGAGGTAACCCCG
			GGAAGGCGGGGATGACGTCAAATCATCATGCCCCTGATGTCCTGGGTCGTAACAAGGTAGCCGTACCGG
			AAGGTGCGGCTG (SEQ ID NO:73)
Bacillus	16S	NCIMB	AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGGATG
lentus		8773	GGAGCTTGCTCCCAGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTACCTGTAAGACTGGG
			ATAACTTCGGGAAACCGGAGCTAATACCGGATAACTTCTTTCTTCTCCTGGAGAAAGGTTGAAAGACGG
			CTTCGGCTGTCACTTACAGATGGGCCCGCGGCGCATTAGCTACTTGGTGAGGTAACGGCTCACCAAGGC
			AACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACG
			GGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAA
			GGTTTTCGGATCGTAAAACTCTGTTATCAGGGAAGAACAAGTATCGGAGTAACTGCCGGTACCTTGACG
			GTACCTGACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG
			TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAA
			CCGTGGAAGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGAAGAGCGGAATTCCACGTGTAGCGG
			TGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGGCTCTTTGGTCTGTAACTGACGCTGAG
			GCGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAA
			GTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCC
			GCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAG
			CAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACCACCCTAGAGATAGGGACTTCCCCTTCGGG
			GGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAA
			CGAGCGCAACCCTTAACCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAAC
			CGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATG
			GATGGTACAAAGGGTTGCAAGACCGCGAGGTTTAGCTAATCCCATAAAACCATTCTCAGTTCGGATTGC
			AGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACG
			TTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAAC
			CCTTACGGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCGTATCG
			GAAGGTGCGGTGGATCA (SEQ ID NO:74)
Halobacillus	16S	NCIMB	GAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGCGGGAAG
halophilus		2269	CAAGCGGATCCTTCGGGGGTGAAGCTTGTGGAACGAGCGGCGGACGGGTGAGTAACACGTGGGCAACCT
			GCCTGTAAGACCGGAATAACCCCGGGAAACCOGGGCTAATGCCGGATAACACCTACCTTCACCTGAAGG
			AAGGTTAAAAGATGGCTTCTCGCTATCACTTACAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGT
			AATAGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAOGGTGATCGGCCACACTGGGACTGAGACAC
			GGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGGAACG
			CCGCGTGAACGATGAAGGTCTTCGGATCGTAAAGTTCTGTTGTTAGGGAAGAACAAGTACCGTACGAAC
			ACAGCGGTACCTTGACGGTACCTAACGAGGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATAC
			GTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTCTTTAAGTCTGATGT
			GAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGGACAGAAGAGGAGAGTGG
			AATTCCACGTGTAGCGGTGAAATGCGTAGATATGTGGAGGAACACCAGAGGCGAAGGCGACTCTCTGGT
			CTGTTTCTGACGCTGAGGTGCGAAAGCGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCG
			TAAACGATGAGTGCTAGGTGTTAGGGGGCTTCCACCCCTTAGTGCTGAAGTTAACGCATTAAGCACTCC
			GCCTGGGGAGTACGGCCGCAAGGNTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCA
			TGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTTGGAACCACCCTAGAGAT
			GGTGTTCCTTCGGGGACCAAGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGG
			GTTAAGTCCCGCAACGAGCGCAACCCCTAATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGAC
			TGCCGGTGACAAACCGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACA
			CACGTGCTACAATGGATGGTACAAAGGGCAGCGAAGCCGCGAGGTGTAGCAAATCCCATAAAACCATTC
			TCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGGTAGTAATCGCGGATCAGCATG
			CCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTGGCAACACCC
			(SEQ ID NO:75)
Bacillus	16S	IAM12468	GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAGCGGAATGACGAGAGCTTGCTCTCGATTTTA
psychrophilus			GCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCCTACAGATGGGGATAACTCCGGGAAACCGGGGC
			TAATACCGAATAATCAGTTTGTCCGCATGGACAAACTCTGAAAGACGGTTTCGGCTGTCACTGTAGGAT
			GGGCCCGCGGCGCATTAGCTAGTTGGTGGGGTAATGGCCTACCAAGGCAACGATGCGTAGCCGACCTGG
			AGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATC
			TTCCACAATGGACGAAAGTCTGATGGAGCAATGCCGCGTGAGCGAAGAAGGTTTTCGGATCGTAAAGCT
			CTGTTGTAAGGGAAGAACACGTACGGGAGTAACTGCCCGTGCCATGACGGTACCTTATTAGAAAGCCAC
			CGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTA
			AAGCGCGCGCAGGCGGTTCTTTAAGTCTGATGTGAAAGCCCACGGCTCACCGTGGAGGGTCATTGGAAA
			CTGGAGAACTTGAGTACAGAAGAGGAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGG
			AGGAACACCAGTGGCGAAGGCGGCTTTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCA
			AACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCC
			CTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGG
			AATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA
			GGTCTTGACATCCCACTGACCGGTGTAGAGATACGCCTTTCCCTTCGGGGACAGTGGTGACAGGTGGTG
			CATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTT
			AGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGATAAACCGGAGGAAGGTGGGGATGAC
			GTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGATACAGAGGGTTGCCA
			ACCCGCGAGGGGGAGCCAATCCCATAAAATCGTTCCCAGTTCGGATTGGAGGCTGCAACTCGCCTCCAT
			GAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCCCGGGTCTTGTACACAC
			CGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTACATCTACGGGAGCCAGCCGCCG
			AAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAA (SEQ ID NO:76)
Paenibacillus	16S	ATCC	TTATTGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGOCGTGCCTAATACATGCAAGTCGAGCGG
macerans		8244	ACCTGATGGAGTGCTTGCACTCCTGATGNNCGGCGGACGGGTGAGTAACACGTAGGCAACCTGCCCGTA
			AGACCGGGATAACTACCGGAAACGGTAGCTAATACCGGATAATCAAGTCTTCCGCATAGGAGACTTGGG
			AAAGGCGGAGCAATCTNTCACTTACGGANNNNNTNCGGCGCATTAGCTAGTTNGTGGGGTAACGGCTTA
			CCAAGGCGACGATGCGTAGCNGACCTGAGAGGGTGAACGGCCACACTGGGACTGAGACACGGCCCAGAC
			TCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAG
			TGATGAAGGTTTTCGGATCGTAAAGCTGNNTTGCCAGGGAAGAACGTCTTCTAGAGTAACTGCNANGAG
			AGTGACGGTACCTGAGAAGAAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCA
			AGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTGTTTAAGTCTGGTGTATAATCCCGG
			GGCTCAACTCCGGGTCGCACTGGAAACTGGACGGCTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTG
			TAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTNGGCTGTAACTGAC
			GCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAG
			TGCTAGGTGTTAGGGGTTTCGATACCCTTGGTGCCGAAGTAAACACATTAAGCACTCCGCCTGGGGAGT
			ACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCAGTGGAGTATGTGGTTTAAT
			TCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGACCGCTGTAGAGATATGGCTTTCTT
			CGGGACAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCG
			CAACGAGCGCAACCCTTGACTTTAGTTGCCAGCAAGTAAAGTTGGGCACTCTAGAGTGACTGCCGGTGA
			CAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTTGNCACACACGTACTAC
			AATGGCCGGTACAACGGGAAGCGAAGTAGTGATATGGAGCGAATCCTAGAAAGCCNGTCNCAGTTCGGA
			TTGCAGGCTGCAACTCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGCG
			AATACGTTCCCGGGTNTTGTACACACCGCCCGTCACACCACGAGAGTTTACAACACCCGAAGTCGGTGA
			GGTAACCGCAAGGGGCCAGCCGCCGAAGGTGGGGTAGATGATTGGGG (SEQ ID NO:77)
Bacillus	16S	ATCC	AACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATTGTTGAGTTTACTCAACAATTAGCGGCGG
psychrosaccha-		23296	ACGGGTGAGTAACACGTGGGCAACCTGCCTATAGACTGGATAACTTCGGGAACCGGAGCTAATACCGAT
rolyticus			ATGTTCTTCTCTCGCATGAGAGAAGATGGAAAGACGGTCTCGGCTGTCACTTATAGATGGGCCCGCGGC
			GCATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCG
			GCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGA
			CGAAAGTCTGACGGAGCAACGCCGCGTGAACGATGAAGGCTTTCOGGTCGTAAAGTTCTGTTGTTAGGG
			AAGAACAAGTACCAGAGTAACTGCTGGTACCTTGAGGTACCTAACCAGAAAGCCACGGCTAACTACGTG
			CCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGT
			GGTTCCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGA
			GTGTAGAAGAGGAAAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTG
			GCGAAGGCGACTTTCTGGTCTATAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGAT
			ACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAG
			CTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGCC
			CGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCT
			CTGACACTCCTAGAGATAGGACGTTCCCCTTCGGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCA
			GCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTC
			AGTTGGGCACTCTAAGGTGACTGCCGGTGATAAACCGGAGGAAGGTGGGGATGACGTCTCATCATGCCC
			CTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGAGCTGCAAACCCGCGAGGGTAAGCG
			ATCTCATAAAGCCATTCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGT
			AATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAG
			AGTTTGTAACACCCGAGTCGGTGAGGTAACCGCAAGGAGCCAGCCCGCCTAAGGTGGGACAGATGATTG
			GGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCA (SEQ ID NO:78)
Bacillus	16S	ATCC	AACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGGATTAAGAGCTTGCTCTTATGAAGTTAG
mycoides		6462	CGGCGGACGGGTGAGTAACACGTGGGTAACCTACCCATAAGACTGGGATAACTCCGGGAAACCGGGGCT
			AATACCGGATAATATTTTGAACTGCATAGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATG
			TGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTG
			AGAGGGTGATCGGCCACACTGGGACTGAGAACGGCCCAGAGTCCTACGGGAGGCAGCAGTAGGGAATCT
			TCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTC
			TGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGAGCGCGCGCAGGTGGTTTCTTAA
			GTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGA
			GGAAAGTGGAATTCCATGTGTAGCGGTGAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGAC
			TTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGT
			CCACGCCGTAAACGATGAGTGCTGAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATT
			AAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAGGAATTGACGGGGGCCCGCACAAGCG
			GTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACTC
			TAGAGATAGAGCTTCTCCTTCGGAGCAGAGTGAAGGTGGTGCATGGTTGTCGTCCTCGTGTCGTGAGAT
			GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATTAGTTGCCATCATTAAGTTGGGCACTCTAAGGT
			GACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCT
			ACACACGTGCTACAATGGACGGTACAAAGAGCGCAAGACCGCGAGGTGGAGCTAATCTCATAAAACCGT
			CTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCAT
			GCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGA
			AGTCGGTGGGGTAACCTTTATGGAGCCAGCCGCCTAAGGTGGGACAGATGATTGGGGTGAATGCGTAAC
			AAGGTAGCCGTATCGGAAGGTGCGGCTGGATCA (SEQ ID NO:79)
Bacillus	16S	DSM 485	GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACCAAAGGGAGCTTGCTCCCAGAGGTT
alcalophilus			AGCGGCGGACGGGTGAGTAACACGTGGNCAACCTGCCCTGTAGACTGGGATAACATCGAGAAATCGGTG
			CTAATACCGGATAATCAAAGGAATCACATGGTTCTTTTGTAAAAGATGGCTCCGGCTATCACTANGGGA
			TGGCCCGCGCGCATTAGCTAGTTGGTAAGGTAATGGCTTACCAAGGCGACGATGCGTAGCCGACCTGAG
			AGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTT
			CCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCT
			GTTGTTAGGGAAGAACAAGTGCCGNTCGAATAGGTCGGCACCTTGACGGTACCTAACCAGAAAGCCACG
			GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAA
			AAGCGCGCGCAGGCGGTCTTTTAAGTCTGATGTGAAATATCGGGGCTCAACCCCGAGGGGTCATTGGAA
			ACTGGGAGACTTGAGTACAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGATATGTG
			GAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGC
			AAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAGGTGTTAGGGGTTTCGATGC
			CCTTAGTGCCGAAGTTAACACATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAG
			GAATTGACGGGGGCCCGCACAAGCAGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC
			AGGTCTTGACATCCTTTGACCACTCTAGAGATAGAGCTTTCCCCTTCGGGGGACAAAGTGACAGGTGGT
			GCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCT
			TAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGA
			CGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACATGGATGGTACAAAGGGAGCGAC
			CGCGAGGTCGAGCCAATCCCATAAAGCCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAA
			GCCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGC
			CCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACGTTTTGGAGCCAGCCGCCTAAGG
			TGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTG (SEQ ID NO:80)
Aneuriniba-	16S	ATCC	GAGAGTTTGATCCTGGCTCAGGNCGANCGCTGGCGGTGTGCCTAATACATGCAAGNCGAGCGGACCAAG
cillus		12856	GAAGAGCTTGCTCTTCGGCGGTTAGCGGCGGACGGGTGAGTAACACGTAGGCAGCCTGCCTGTACGACT
aneurinilyti-			GGGATAACTCCGTGAAACCGGAGCTAATACCAGATACGTTTTTCAGACCGCATGGTCTGAAAGAGAAAG
cus			ACCTCTGGTCACGTACAGATGGGCCTGCGGCGCATTAGCTAGTTGGTGGGGTAACGGTCTACCAAGGCG
			ACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGG
			GAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAACGATGAAG
			GTTTTCGGATCGTAAAGTTCTGTTGTTAGGGAAGAACCGCCGGGATGACCTCCCGGTCTGACGGTACCT
			AACGAGAAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGA
			ATTGGGCGTAAAGCGCGCGCAGGCGGCTTCTTAAGTCAGGTGTGAAAGCCCACGGNTCAACCGTGGAGG
			GCCACTTGAAACTGGGAGGCTTGAGTGCAGGAGAGGAGAGCGGAATTCCACGTGTAGCGGTGAAATGCG
			TAGAGATGTGGAGGAACAACCGTGGCGAAGGCGGCTCTCTGGCCTGTAACTGACGCTGGGGCGCGAAAG
			CGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGAAAACGTTGAGTGTTAGGTGTTGGGG
			ACTCCAATCCTCAGTGCCGCAGCTAACGCAATAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAA
			ACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGA
			ACCTTGCCAGGGCTTGACATCCCGCTGTCCCTCCTAGAGATAGGAGNTCTCTTCGGAGCAGCGGTGACA
			GGTGGTGCATGGTTGTCGNCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCT
			TGTCCTTAGTTGNCAGCATTCAGTTGGGCACTCTAGGGAGACTGCCGTCGACAAGACGGAGGAAGGTGG
			GGATGACGTCAAATCATCATGCCCCTTATGTCCTGGGCTACACACGTGCTACAATGGATGGAACAACGG
			GCAGCCAACTCGCGAGAGTGCGCCAATCCCTTAAAACCATTCTCAGTTCGGATTGCAGGCTGCAACCTC
			GCCTGCATGAAGCCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTT
			GTACACACCGCCCGTCACACCACGAGAGTTTGCAACACCC (SEQ ID NO:81)
Amphibacillus	16S	DSM 6626	ATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGCGTCNNATTAAAACAGA
xylanus			TCTCTTCGGAGTGACGTTTAATGGATCGAGCGGCGGATGGGTGAGTAACACGTGGCCAACCTGCCTATAA
			GACTGGGATAACTTACGGAAACGTGAGCTAATACCGGATAAAACCTTTTGTCTCCTGACAAGAGGATAAA
			AGATGGCGCAAGCTATCACTTATAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGATAAAAGCTCACC
			AAGCCACGATGCGTAGCCGACCTGAGAGGGTGATTGGCCACACTGGGACTGAGATACGGCCCGATCCTAC
			GGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAACGAAGAA
			GGTCTTCGGATCGTAAAGTTCTGTTGTTAGGGAAGAACACGTACCATTCGAATAGGGTGGTACCTTGACG
			GTACCTAACGAGAAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGT
			CCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTCTTTAAGTCTGATGTGAAATCTTGCGGCTCAACC
			GCAAGCGGTCATTGGAAACTGGAGAACTTGAGGACAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGA
			ATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCG
			AAAGCGTGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAGGTGTTA
			GGGGGTTTCCCCCCCTTAGTGCTGGCGTTAACGCATTAAGCACTCCNCCTGGGGAGTACGGCCGCAAGGC
			TGAAACTCAAAAGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGA
			AGAACCTTACCAGGTCTTGACATCCCGCTGACCGCTATGGAGACATAGCTTTCCCTTCGGGGACAGCGGT
			GACAGGTGGTGCATGGTTGTCGTTGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC
			CTTGAACTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGTTGACTGCCGGTGACAAACCGGAGGAAGGTT
			GGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGNTTGGTAGTTCG
			GATTGTCGGTTGAACTCGCCTACATGAAGCCGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAA
			TACGTTCCCGGGTCTTGTACACACCGTCCGTCACACCACGAGAGTTAGCAACACCCGAAGTCGGTGAGGT
			AACGCTTTTAGNGAGCCAGCCGCCGAAGGTGGGGCCAATGATTGGGGTGAAGTCGTAACAAGGTAGCCGT
			ATCGGAAGGTGCGGNTGGATCACCTCCTT (SEQ ID NO:82)
Bacillus	16S	IAM	GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGCGGGAACNAAGCAGATCTCCTTCGGGGGT
pantothenticus		11061	GACGCTTGTCCAACGGACGGGTGAGTAACACGTGGGCAACCTACCTGTAAGACTGGGATAACTCCGGGAA
			ACCGGGGCTAATACCGGATGATACATATCGTCCATACGAGATGTTGAAAAGGCGGCATATGCTGTCACTT
			ACAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGATAAAAGCTCACCAAGGCGACGATGCGTAGCCGA
			CCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGA
			ATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAA
			CTCTGTTGTTAGGGAAGAACAAGTGCCATTCGAATAGGTTGGCACCTTGACGGTACCTAACCAGAAAGCC
			CCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTA
			AAGCGCGCGCAGGCGGTCCTTTAAGTCTGATGTGAAAGCCCACGGCTTAACCGTGGAGGGCCATTGGAAA
			CTGGGGGACTTGAGTACAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGA
			GGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGTGCGAAAGCGTGGGTAGCGAA
			CAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAGGTGTTAGGGGGTTTCCGCCCCTT
			AGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATT
			GACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCT
			TGACATCCTCTGACGCCCCTAGAGATAGGGAGTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGG
			TGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCT
			ACACACGTGCTACAATGGATGGAACAAAGGGCAGCGAAGCCGCGAGGCCAAGCAAATCCCATAAAACCAT
			TCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGTAATCGCGGATCAGCAT
			GCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTGGTAACACCCGAA
			GTCGGTGAGGTAACCTTTTGGAGCCAGCCGCCGAAGGTGGGACTAATGATTGGGGTGAAGTCGTAACAA
			(SEQ ID NO:83)
Paenibacillus	16S	ATCC	TGCCTAATACATGCAAGTCGAGCGGACTCAACTGTTTCCTTCGGGAAACCGTTAGGTTAGCGGCGGACGG
popilliae		14706	GTGAGTAATACGTAGGTAACCTGCCCTTAAGACYGGGATAACTCACGGAAACGTGGGCTAAWACCGGATA
			GGCGATTTGCTCGCATGAGGGAATCGGGAAAGGCGGAGCAATCTGCCACTTATGGATGGACCTACGGCGC
			ATTAGCTAGTTGGTGRGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCC
			ACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGCA
			AGTCTGACGGAGCAACGCCGCGTGAGTGATGAACGTTTTCGGATCGTAAAGCTCTGTTGCCAGGGAAGAA
			CGCTATGGAGAGTAACTGTTCCATAGGTGACGATACCTGAGAAGAAAGCCCCGGCTAACTACGTGCCAGC
			AGCCGCGGTAATACGTAGCGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTCA
			TGTAAGTCTGGTGTTTAAACCCGGGGCTCAACTCCGGGTCGCATCGGAAACTGTGTGACTTGAGTGCAGA
			AGAGGAAAGTGGAATTCCACGTGTAGCGGTGATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGA
			CTTTCTGGGCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAACAGGATTAGATACCCTGGTAGTC
			CACGCCGTAAACGATGAATGCTAGGTGTTAGGGGTTTCGATACCCTTGGTGCCGAAGTTAACACATTAAG
			CATTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCAGTG
			GAGTATGTGGTTTAATTCGAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGACCGCGCTAGA
			GATAGGGCTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTOTCGTGAGATGT
			TGGGTTAAGTCCCGCAACGAGCGCAACCCTTAACTTTAGTTGCCAGCATTGAGTTGGGCACTCTAGAGTG
			ACTGCCGGTGAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACAC
			ACGTACTACAATGGCTGGTACAACGGGAAGCGAAGCCGCGAGGTGGAGCGAATCCTAAAAAGCCAGTCTC
			AGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCG
			CGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTTACAACACCCGAAGTCG
			GTGGGGTAACCGCAAGGAGCCAGCCGCCGAAGGTGGGGTAGATGATTGGGGTGAAGTCGTAACAA
			(SEQ ID NO:84)
B. cereus	cpn60		GCAACTGTATTAGCGCAAGCTATGATTCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCGAACCCAATGG
			GGCTTCGTAAAGGTATCGAAAAAGCTGTTACTGCTGCAATTGAAGAATTAAAAACGATTTCTAAACCAAT
			CGAAGGCAAATCTTCTATCGCACAAGTAGCTGCTATTTCTGCAGCTGACGAAGAGTAGGTCAATTAATCG
			CTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATTACTTTAGAAGAGTCTAAAGGATTCACAACAGA
			ATTAGACGTAGTAGAAGGTATGCAATTTGATCGTGGATATGCATCTCCTTACATGATTACTGATTCTGAC
			AAAATGGAAGCAGTTCTTGATAACCCATACATCTTAATTACTGACAAAAAGATTTCTAACATTCAAGAAA
			TCTTACCAGTATTAGAGCAAGTGGTACAACAAGGTAAACCACTTCTTATCATTGCTGAAGATGTAAAAGG
			CGAAGCTTTAGCTACATTAGTAGTGAACAAACTTCGTGGTACATTCAACGTAGTAGCTGTT
			(SEQ ID NO:85)
.thuringien-	Cpn60		GCAACTGTATTAGCGCAAGCTATGATTCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCGAACCCAATGG
sis var.			GTCTTCGTAAAGGTATCGAAAAAGCTGTTACTGCTGCAATTGAAGAATTAAAAACGATTTCTAAACCAAT
kurstaki			CGAAGGTAAATCTTCTATCGCACAAGTAGCTGCTATTTCTGCTGCTGACGAAGAAGTAGGTCAATTAATC
HD1 (=B51 B.			CTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATTACTTTAGAAGAATCTAAAGGCTTCACAACAGA
anthracis)			ATTAGACGTAGTAGAAGGTATGCAATTTGATCGTGGATATGCATCTCCTTACATGATTACTGATTCTGAC
			AAAATGGAAGCAGTTCTTGATAACCCATACATCTTAATCACTGACATAAGATTTCTAACATTCAAGAAAT
			CTTACCAGTATTAGAGCAAGTGGTACAACAAGGTAAACCACTTCTTATCATTGCTGAAGATGTAGAGGCG
			AGCGTTAGCTACATTAGTAGTGAACAAACTTCGTGGTACATTCAATGTAGTAGCTGTT
			(SEQ ID NO:86)
B. subtilis	cpn60		GCAACAGTTCTTGCGCAAGCAATGATCCGTGAAGGCCTTAAAAACGTAACAGCAGGCGCTAACCCTGTAG
168			GCGTGCGTAAAGGGATGGAACAAGCTGTAGCGGTTGCGATCGACTTAGAAATTTCTAAGCCAATCGAAGG
			CAAAGAGTCTATCGCTCAGGTTGCTGCGATCTCTGCTGCTGATGAGGAAGTCGGAAGCCTTATCGCTGAA
			GCAATGGAGCGCGTAGGAAACGACGGCGTTATCACAATCGAAGAGTCTAAAGGCTTCACAACTGAGCTTG
			ACTGAGCTTGAAGTTGTTGAAGGTATGCAATTCGACCGCGGATATGCGTCTCCTTACATGGTAACTGACT
			CTGATAAGATGGAAGCGGTTCTTGACAATCCTTACATCTTAATCACAGACAAAAAAATCACAAACATTCA
			AGAAATCCTTCCTGTGCTTGAGCAGGTTGTTCAGCAAGGCAAACCATTGCTTCTGATCGCTGAGGATGTT
			GAAGGCGAAGCACTTGCTACACTTGTTGTGAACAAACTTCGCGGCACATTCAACGCAGTGGCTGTT
			(SEQ ID NO:87)
B. subtilis	cpn60		GCGACAGTTCTTGCGCAAGCAATGATCCGTGAAGGCCTTAAAAACGTAACAGCAGGCGCTAATCCTGTAG
W235R			GCGTTCGTAAAGGTATGGAAAAAGCTGTAGCGGTTGCGATCGAAAACTTAAAAGAAATTTCTAAGCCAAT
			CGAAGGCAAGGAGTCTATCGCTCAGGTTGCTGCGATCTCTGCTGCTGAGGAGGAAGTCGGAAGCCTTATC
			GCTGAAGCAATGGAGCGCGTAGGCAACGACGGCGTTATCACAATCGAAGAGTCTAAAGGCTTCACAACTG
			AGCTTGAAGTTGTTGAAGGTATGCAATTCGACCGCGGATATGCGTCTCCTTACATGGTAACTGACTCTGA
			TAAGATGGAAGCGGTTCTTGACAATCCTTACATCTTAATCACAGACAAAAAAATCACAAACATTCAAGAA
			ATCCTTCCTGTACTTGAGCAGGTTGTTCAGCAAGGCAAACCATTGCTTCTAATCGCTGAGGATGTTGAAG
			GCGAAGCACTTGCAACACTTGTTGTGAACAAACTTCGCGGTACATTCAACGCAGTTGCTGTT
			(SEQ ID NO:88)
B. licheni-	cpn60	ATCC14580	GCGACAGTTCTAGCTCAGGCGATGATTCGCGAAGGTCTTAAAAACGTAACTGCCGGCGCTAACCCTGTAG
formis			GCGTGCGTATCGAGCAGGCTGTGGCTGTAGCTGTTGAAAGCCTGAAAGAAATCTCTAAACCAATTGAAGG
			CAAAGAATCAATCGCACAAGTTGCTTCAATCTCCGCTGCAGACGAAGAAGTCGGAAGCCTGATCGCTGAA
			GCAATGGAGCGCGTCGGCAACGACGGTGTTATCACGATCGAAGAATCCAAAGGATTCACAACAGAGCTTG
			AAGTGGTTGAAGGTATGCAGTTCGACCGCGATATGCGTCTCCTTACATGGTGACGGATTCCGATAAGATG
			GAAGCGGTTCTTGAGAATCCGTACATCTTAGTAACAGACAAAAAAATCACAAACATTCAAGAAATCCTGC
			CGGTGCTTGAGCAAGTCGTGCAACAAGGCAAACCGTTGCTTCTGATTGCTGAAGACGTTGAAGGTGAAGC
			TCTTGCAACATTGGTTGTCCAAGCTTCGCGGAACATTCAACGCAGTGGCTGTT (SEQ ID NO:89)
B. pumilus	cpn60		GCGACTGTACTTGCGCAGGCTATGATCCGCGAAGGCCTTAAAAACGTAACTGCGGGGGCTAACCCTGTCG
B205-L M&G			GCGTGCGTAAAGGTATGGAACA AGCCGTGACTGTAGCAATCGAAAACTTAAAAGAAATTTCTAAGCCGA
			TCGAAGGCGAGTCTATCGCTCAGGTTGCTGCGATCTCTGCTGCTGATGAGGAAGTCGGAAGCCTTATCGC
			TGAAGCAATGGAGCGCGTAGTAAACGACGGCGTCATCACAATCGAAGAGTCTAAAGGTTTCACA
			ACTGAGCTTGAAGTTGTTGAAGGTATGCAATTCGACCGCGATATGCGTCTCCTTACATGGTGACTGACTC
			TGATAAGATGGAAGCGGTTCTTGACAATCCTTACATCTTAATCACAGACAAAAAAATCACAAACATTCAA
			GAAATCCTTCCTGTGCTTGAGCAAGTTGTACAGCAAGGCAAACCATTGCTTCTGATCGCTGAAGATGTTG
			AAGGGGAAGCTCTTGCTACACTCGTTGTCAACAAACTTCGCGGCACATTCAACGCTGTTGCCGTT
			(SEQ ID NO:90)
B. pumilus	cpn60	ATCC7061	GCAACAGTTCTAGCTCAAGCGATGATCCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCAAACCCTGTTG
			GCGTTCGTAAAGGGTATCGAAGAAGCCGTGACTGTAGCAATCGAAAACTTAAAAGAAATTTCTAAGCCGA
			TCGAAGGCGTTCGTAAAGGGATCGAAGAAGTTGGAAGCCTGATCGCTGAAGCAATGGAGCGTGTAGGTAA
			CGACGGCGTGATCACAATCGAAGAATCTAAAGGGTTCACAACTGAGCTTGAAGTGGTTGAAGGATGCAGT
			TTGACCGAGGATATGCTTCACCATACATGGTGACGCTGATAAGATGGAAGCGGTTCTTGAAAATCCTTAC
			ATCTTAATCACTGATAAAAAAATCACAAACATTCAAGAAATCCTTCCTGTACTTGAGCAAGTTGTACAAC
			AAGGAAAACCATTATTGCTCATTGCTGAAGATGTAGAAGGCGAAGCACTTGCAACACTTGTTGTGAACAA
			ACTTCGTGGAACATTCAACGCAGTGGCAGTA (SEQ ID NO: 91)
B. amylolique-	cpn60		GCGACTGTGCTTGCACAGGCTATGATCCGCGAAGGCCTTAAACGTAACTGCGGGAGCTAATCCTGTCGGC
faciens H			GTGCGTAAAGGTATGGAACAAGCCGTAACCGTGGCAATCGAAAACTTAAAAGAAATTTCTAAGCCGATCG
			AAGGCAAAGAGTCTATCGCTCAGGTTGCTGCAATCTCTGCTGCTGATGAGGAAGTCGGAAGCCTTATCGC
			TGAAGCAATGGAGCGCGTAGGAAACGACGGCGTTATCACAATCGAAGAGTCTAAAGGCTTCACAACTGAG
			CTTGAAGTGGTTGAAGGTATGCAATTCGACCGCGGATATGCGTCTCCTTACATGGTTGACTGACTCTGAT
			AAGATGGAAGCGGTTCTTGATAATCCTTACATCTTAATCACAGACAAAAAAATCACAAACATTCAAGAAA
			TCCTTCCTGTGCTTGAGCAAGTTGTACAGCAAGGCAAACCATTGCTTCTGATCGCTGAAGATGTTGAAGG
			TGAAGCTCTTGCTACACTCGTTGTCAACAAACTTCGCGGCACATTCAACGCTGTTGCCGTT
			(SEQ ID NO:92)
B. amylolique-	cpn60		GCAACTGTATTAGCACAAGCTATGATTCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCGAACCCAATGG
faciens N			GTCTTCGTAAAGGTATCGAAAAAGCTGTAGTTGCTGCAGTAGAAGAATTAAAAACGATTTCTAAACCAAT
			CGAAGGTAAATCTTCAATCGCACAAGTAGCTGCTATTTCTGCGGCTGACGAAGAAGTAGGTCTTTAATC
			GCTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATTACTTTAGAAGAATCTAAAGGATTCACAACAG
			AATTAGATGTAGTAGAAGGTATGCAATTTGATCGTGGATATGCATCTCCTTACATGATTACTGATTCTGA
			CAAAATGGAAGCAGTTCTTGATAACCCATACATCTTAATCACTGACAAAAAGATTTCTAACATTCAAGAA
			ATCTTACCAGTATTAGAGCAAGTGGTACAACAAGGTAAACCGCTTCTTATCATTGCTGAAGATGTAGAAG
			GCGAAGCATTAGCTACATTAGTAGTGAACAAACTTCGTGGTACATTCAATGTAGTAGCTGTT
			(SEQ ID NO:93)
B. globigii	cpn60		GCTACAGTTCTTGTTCAGGCTATGATTCGTGAAGGTCTTAAAAACGTAACGGCAGGCGCTAACCCTGTAG
SB512			GCGTTCGTAAAGGTATGGAACAAGCTGTAACAGTTGCGATTCAAACCTTCAAGAAATCTCTAAACCGATC
			GAAGGAAAAGAGTCTATCGCTCAGGTTGCTGCGATTTCTGCTGCTGATGAAAAAGTCGGAAGCCTGATTG
			CTGAAGCGATGGAGCGCGTTGGAAACGACGGCGTTATCACGATCGAAGAATCTAAAGGTTTCACAACTGA
			GCTTGAAGTTGTTGAAGGTATGCAGTTCGACCGCGGATATGCATCTCCTTACATGGTAACTGATTCTGAT
			AAGATGGAAGCGGTTCTTGAAAATCCTTACATCTTAATCACAGACAAAAAAATTACAAATATTCAAGAAA
			TCCTTCCTGTGCTTGAGCAGGTTGTTCAGCAAGGCAAACCATTGCTTCTGATTGCTGAGGATGTTGAAGG
			TGAAGCTCTTGCAACACTTGTTGTGAACAAACTTCGCGGCACATTCAACGCAGTTGCCGTT
			(SEQ ID NO:94)
G. stearother-	cpn60		GCAACAGTTTTAGCGCAAGCAATGATCCGCGAAGGATTGAAAAACGTTACAGCTGGCGCTAACCCAATGG
mophilus			GCATCCGTAAAGGTATTGAAAAAGCGGTCGCTGTGGCAGTAGAAGAATTAAAAGCAATCTCCAAACCAAT
BGSC strain			TCAAGGTAAAGAATCGATTGCTCAAGTTGCAGCGATCTCTGCGGCTGACGAAGAAGTTGGTCAATTAATC
9A2			GCAGAAGCAATGGAACGCGTTGGCAACGATGGTGTTATCACATTAGAAGAATCGAAAGGCTTCGCAACGG
			AATTAGATGTTGTCGAAGGTATGCAATTTGACCGTGGTTATGTATCTCCATACATGATCACAGATACAGA
			AAAAATGGAAGCAGTGCTTGAAAATCCATACATCTTAATTACAGATAAAAAAGTTTCTAGCATCCAAGAA
			ATCTTGCCTATCTTAGAACAAGTAGTTCAACAAGACCGCTATTAATTATCGCAGAAGATGTCGAAGGCGA
			AGCGCTCGCAACATTAGTCGTCAACAAACTTCGTGGTACATTCAATGCGGTAGCGGTA
			(SEQ ID NO:95)
B. megaterium	cpn60		GCAACAGTTTTAGCGCAAGCAATGATCAGAGAAGGTCTTAAAAACGTAACGGCTGGTGCTAACCCAATGG
899			GTATCCGTAAAGGTATGGAAAAGGCAGTAGCTGTAGCGGTTGAAGAACTAAAAGCAATCTCTAAACCAAT
			TCAAGGTAAAGATTCAATTGCTCAAGTAGCGGCTATCTCAGCAGCTGACGAAGAAGTAGGTCAATTAATT
			GCTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATCACACTTGAAGAATCAAAAGGTTTCACAACTG
			AATTAGAAGTGGTAGAAGGTATGCAGTTTGACCGTGGATATGCATCTCCTTACATGGTAACTGATTCAGA
			TAAAATGGAAGCTGTATTAGATGATCCATACATCTTAATCACAGACAAAAAAATCGGTAACATTCAAGAA
			ATCTTACCGGTATTAGAGCAAGTTGTTCAACAAGGCAAGCCTCTATTGATCATCGCTGAAGACGTAGAAG
			GCGAAGCTTTAGCAACATTAGTTGTGAACAAACTTCGTGGTACATTCACAGCTGTAGCTGTT
			(SEQ ID NO:96)
B. megaterium	cpn60	ATC19213	GCAACTGTATTAGCGCAAGCTATGATTCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCTAACCCAATGG
bgsc			GTCTTCGTAAAGGTATCGAAAAAGCTGTTACTGCTGCAATTGAAGAATTAAAAACGATTTCTAAACCAAT
			CGAAGGCAAATCTTCTATCGCACAAGTAGCTGCTATTTCTGCAGCTGACGAAGAAGTAGGTCAATTAATC
			GCTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATTACTTTAGAAGAGTCTAAAGGATTCACAACAG
			AATTAGACGTAGTAGAAGGTATGCAATTTGATCGTGGATATGCATCTCCTTACATGATTACTGATTCTGA
			CAAAATGGAAGCAGTTCTTGATAACCCATATATCTTAATTACTGACAAAAAGATTTCTAACATTCAAGAA
			ATCTTACCAGTATTAGAGCAAGTGGTACAACAAGGTAAACCACTTCTTATCATTGCTGAAGATGTAGAAG
			GGCGAAGCTTTAGCTACCATTAGTAGTGAACAAACTTCGTGGTCATTCAATGTAGTAGCTGTT
			(SEQ ID NO:97)
B. coagulans	cpn60		GCGACCGTTCTGGCCCAGGCAATGATCCGTGAAGGCCTGAAAAACGTAACAGCAGGCGCAAACCCGGTTG
CECT12			GCATCCGCAAAGGGATTGAAAAAGCGGTTGCGGCTGCTGTTGAAGAATTAAAAGCCATTTCGAAACCAAT
			CGAAGGCAAAGCTTCCATCGCCCAAGTTGCTGCAATTTCCTCTGCTGACGAAGAAGTTGGCGAATTGATC
			GCTGAAGCAATGGAACGCGTGGGCAACGACGGCGTCATTACCATTGAAGAATCAAAAGGCTTCTCAACGG
			AATTGGACGTTGTGGAAGGGATGCAGTTTGACCGTGGCTATGCATCGCCTTACATGGTAACGGATTCCGA
			CAAAATGGAAGCTGTTCTGGATAACCCTTATATCTTAATTACAGACAAGAAGATTTCCAATATCCAGGAA
			ATCCTCCCTGTTCTCGAACAAGTTGTCCAACAAGGCAAACCGCTGTTGCTGATTGCGGAAGATGTTGAAG
			GGGAAGCTCTTGCAACACTCGTTGTCAACAAACTGCGTGGCACATTCAATGCAGTTGCGGTG
			(SEQ ID NO:98)
A. acidocal-	cpn60		GCGACGGTGCTGGCGCAGGCGATGATCCGCGAGGGTCTGAAGAACGTCGCCGCTGGTGCGAACCCGATGG
darius			TGCTCCGCCGCGGCATTGAGAAGGCCGTGACGGCTGCGGTCGAGGAGCTGAAGAAGATCGCGAAGCCGGT
CECT4328			CCAGGGCCGCAAGAACATCGCGGAGGTTGCCGCCATCTCGGCTGGTTCGAACGAAATCGGCGAGCTCATC
			GCGGATGCGATGGAGAAGGTTGGCAACGACGGCGTGATCACCGTCGAAGAGTCGAAGGGCTTCACGACCG
			AGCTTGAGGTCGTCGAGGGTATGCAGTTCGACCGCGGCTACATCTCGCCGTACATGGTGACGGACGCGGA
			CAAGATGGAGGCTGTGCTGGACGAGCCGCTCATCCTCATCACCGACAAGAAGGTCTCGAGCATCCAGGAG
			ATCCTGCCGGTGCTGGAGCGCGTCGTGCAGGCTGGCCGTTCGCTGCTCCTCATCGCCGAGGATGTGGAGG
			GCGAAGCGCTCGCGACGCTCGTGGTCAACAAGATCCGCGGTACGTTCAACGCCGTGGCCGTCAAA
			(SEQ ID NG:99)
B. lentus	cpn60		GCAACTGTTCTTGCACAAGCAATGATCCGTGAAGGCTTGAAAAACGTAACTGCTGGAGCTAATCCTGTTG
CECT 18			GCGTTAAAAAAGGGATGGAAAAAGCAGTTGCAACAGCAGTAACTGAGCTACAAACTATCTCAAAACAAAT
			TGAAGATAAAGAATCAATTGCTCAAGTTGCATCTATTTCTTCTGGTGACGAAGAAGTTGGCCAATTAATA
			GCTGAAGCAATGGAACGTGTTGGTAATGATGGCGTTATTACAATTGAAGAGTCTCGTGGTTTCACTACAG
			AGCTTGAAGTTGTAGAAGGAATGCAGTTCGACCGTGGTTATGCATCTCCTTATATGGTAACAGATTCTGA
			TAAAATGGAAGCTGTGCTTGAAAATCCATATATCTTGATCACAGATAAGAAAATTACAAACATCCAAGAA
			GTACTACCTGTTCTTGAGCAAGTTGTTCAACAAGGTAAACCATTGTTGATGATTGCTGAAGATGTAGAAG
			GTGAAGCACTTGCTACACTTGTAGTAAACAAACTTCGCGGAACATTCAACGCAGTAGCTGTT
			(SEQ ID NO:100)
H. halophilus	cpn60		GCAACCGTACTAGCGCAAGCGATGATCCGTGAAGGTCTAAAAAACGTAACATCCGGTGCGAACCCAGTAG
			GCATTCGCCGCGGAATTGAAAAAGCAACCGAAGTCGCTACTCAGGAACTTCGCAAAATCTCTAAGCCAAT
			CGAAGGCCGCGAGTCCATTTCTCAGGTAGCTTCCATCTCTGCTTCCGATAACGAAGTCGGCCAGCTGATT
			GCTGAAGCGATGGAGCGCGTAGGAAACGATGGCGTTATTACAATTGAAGAATCTAAAGGTTTCAATACAG
			AACTAGAAGTGGTTGAAGGTATGCAGTTCGACCGCGGCTATGCTTCTCCATACATGGTTACAGACCAGGA
			TAAAATGGAAGCGGTTCTTGATGATCCTTACATTCTAATTACGGATAAGAAAATCAACAACATCCAGGAA
			GTACTTCCTGTACTTGAGCAAGTGGTACAGCAATCCAAGCCGTTGCTACTGATCTCTGAAGACGTAGAAG
			GCGAAGCACTTGCTACACTTGTTGTGAACAAACTGCGCGGTACATTCAACGCTGTATCCGTT
			(SEQ ID NO:101)
B. marinus	cpn60		GCAACTGTTCTTGCTCAAGCAATGATCCGTGAAGGTCTTAAAAACGTTACAGCTGGTGCAAACCCAGTTG
			GCGTTCGTAAAGGAATTGAAAAAGCGGTTCAATCAGCACTTGTTGAGCTTAAAGAGATCTCAAAACCGAT
			TGAAGGCAAAGAGTCGATTGCACAAGTTGCAGCTATCTCTTCATCAGATGAAGAAGTAGGGCAATTGATT
			GCTGAAGCAATGGAGCGCGTTGGTAACGATGGCGTGATTACAATCGAAGAATCAAAAGGCTTCACAACTG
			ACTGGATGTAGTAGAAGGTATGCAATTTGACCGTGGATATGCATCACCGTACATGGTAACAGATTCAGAT
			AAAATGGAAGCAGTTTTAGAAAATCCATATATCTTAATCACAGACAAGAAAATCGGTAACATCCAAGAAG
			TGCTTCCTGTACTTGAGCAAGTTGTACAACAAGGTAAGCCACTATTGATTGTTGCTGAAGATGTTGAAGG
			CGAAGCACTAGCAACACTTGTTGTGAACAAACTACGTGGAACATTCAACGCAGTAGCTGTC
			(SEQ ID NO:102)
S. psychrophila	cpn60		GCAACAGTTCTAGCGCAAGCAATGATCCGTGAAGGACTGAAAAACGTAACTGCAGGTGCTAACCCTGTC
CECT4073			GGAATCCGTAAAGGAATCGAAAAAGCGGTTATAGCTGCTGTTGAAGGCCTTCAAGAATCTCCAATGAAA
			TCGAAGGAAAAGAAGAGATTGCACAAGTCGCATCTATTTCTTCTGGAGACGAAGAAGTTGGGAAACTTA
			TTGCTGAAGCAATGGAGCGCGTTGGCAACGATGGTGTCATTACTATCGAAGAGTCAAAAGGCTTCACGA
			CTGAACTAGACGTTGTTGAAGGAATGCAATTTGACCGCGGTTATGCATCTGCATACATGGCAACGGATA
			CAGACAAAATGGAAGCAGTTTTGGACAATCCGTATATCTTGATCACAGATAAAAAGATTACGAACATCC
			AAGAAATTCTTCCTGTTCTTGAGCAAGTAGTTCAACAAGGTAAGCCACTTCTTATGATCGCAGAAGACG
			TTGAAGGCGAAGCACTTGCAACACTTGTTGTGAACAAACTACGTGGTACATTCAATGCTGTTGCTGTT
			(SEQ ID NO:103)
P. macerans	cpn60		GCAACAGTTCTTGCTCAGGCAATGATCCGTGAAGGCCTTAAGAACGTAACTGCAGGTGCTAACCCAATGG
CECT19			GCATCCGCAAAGGAATTGAAAAAGCGGTTTCTACTGCTGTTGAAGAGTTAAAAGCTATTTCAAAACCTAT
(= B58			CGAAAACAAAGAATCTATCGCACAGGTTGCTGCTATTTCTGCTGCTGACAATGAAGTTGGCCAGCTGATC
B. firmus			GCTGAAGCAATGGAGCGCGTTGGCAACGATGGTGTTATCACAATCGAAGAATCTAAAGGTTTCACAACTG
			AGCTTGATGTGGTAGAAGGTATGCAATTCGACCGCGGATACGCTTCACCATACATGGTTACAGATTCTGA
			TAAGATGGAAGCGGTTCTTGAAAACCCTTATATCTTAATCACTGATAAGAAGATCACAAGCATCCAGGAA
			ATTCTTCCTGTACTTGAGCAGGTTGTACAGCAAGGCAAGCCTTTATTGCTTGTAGCTGAGGATGTTGAAG
			GTGAAGCACTAGCTACATTAGTAGTGAATAAGCTTCGTGGAACTTTCAACGCTGTAGCGGTT
			(SEQ ID NO:104)
B. psychro-	cpn60		GCTACTGTCCTTGCACAAGCTATGATTCGTGAAGGCCTGAAAAACGTAACGGCTGGCGCGAATCCTATGG
saccharolyticus			GCATTCGTAAAGGGATTGAAAAAGCTGTGAAAGCTGCAATTAGTGAGTTACAAGCTATCTCTAAACCAAT
CECT			CGAAAACAAAGAGTCTATTGCACAAGTTGCAGCAATCTCAGCTTCTGACGAAGAAGTGGGTCAATTAATT
4074			GCTGAAGCAATGGAACGCGTTGGCAACGACGGTGTTATCACAATTGAAGAGTCTAAAGGATTCTCAACTG
			AATTGGACGTAGTAGAAGGTATGCAGTTCGACCGTGGATATGCATCTGCTTATATGGTAACAAACCCAGA
			TAAAATGGAAGCAGTTCTTGAAAATCCATATATCTTAATTACTGACAAAAAAATCTCAAACATTCAAGAA
			ATTCTTCCTGTACTTGAACAAGTTGTTCAACAAGGAAAATCTCTATTGCTAATTGCTGAAGACATTGAAG
			GCGAAGCACTATCAACACTTGTTGTGAACAAACTTCGTGGAACATTCAATGCAGTTGCTGTA
			(SEQ ID NO:105)
B. mycoides	cpn60		GCAACTGTATTAGCGCAAGCTATGATTCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCAAACCCAATGG
CECT 4128			GTCTTCGTAAAGGTATCGAAAAAGCTGTTACTGCTGCAATTGAAGAATTAAAAGCGATTTCTAAACCAAT
			CGAAGGTAAATCTTCTATCGCACAAGTAGCTGCTATTTCTTCGGCTGACGAGAAGTAGGTCAATTAATC
			GCTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATTACTTTAGAAGAATCTAAAGGATTCACAACAG
			AATTAGACGTAGTAGAAGGTATGCAATTTGATCGTGGATAAGCATCTCCTACATGATTACTGATTCTGAC
			AATGAGAGTTCACTTCTTATCATTGCTGAAGATGTAGAAGGCGAAGCGTTAGCTACATTAGTAGTGAACA
			AACTTCGTGGTACATTCAATGTAGTTGCTGTT (SEQ ID NO:106)
B. alcalophilus	cpn60		GCGACTGTTCTAGCTCAAGCGATGATTCGTGAAGGTCTTAAAAACGTAACATCTGGTGCGAACCCAATGG
CECT 1			GTATCCGTAAAGGGATTGAAAAAGCAACAGCTGCTGCGGTTACAGAACTTAAAAATATTGCGAAACCAAT
			CGAAGGCAAAGAGTCAATCGCACAAGTTGCGGCTAACTCAGCAGCTGACGAAGAAGTTGGACAAATTATC
			GCAGAAGCAATGGAACGTGTTGGAAACGACGGCGTTATTACAATCGAAGAATCAAAAGGTTTCTCTACTG
			AATTAGAAGTAGTAGAAGGTATGCAATTCGATCGTGGTTTCGTTTCTCCATACATGGTAACCGATTCTGA
			CAAAATGGAAGCAGTTCTTGAAAATCCATATATTTTAATTACGGATAAAAAGATTGCAAGCATTCAAGAA
			ATCCTACCAGTTCTTGAGCAAGTGGTTCAACAAGGTAAACCAATCCTAATCATCGCTGAAGATGTTTGAA
			GGGGAAGCTCAAGCAACATTAGTTGTTAATAAATTACGTGGTACATTCAATGCGGTAGCCGTT
			(SEQ ID NO:107)
A. aneruino-	cpn60	ATCC12856	GCTACAGTTCTTGCTCAAGCGATGATTCGCGAAGGCTTGAAAAACGTAACAGCGGGTGCAAACCCGATGG
lyticus			TTATGCGCAAAGGTATCGAAAAGGCAGTTCGTGCAGCAGTAGAAGAACTGCATGCGATTTCTAAACCAAT
			CGAAGGTAAAGAATCTATCGCACAAGTAGCAGCTATTTCTGCTGCTGATGAGGAAATCGGCCAACTGATT
			GCTGAAGCTATGGAAAAAGTAGGAAAAGATGGCGTTATCACAGTAGAAGAATCCAAAGGCTTCACAACAG
			AACTTGATGTTGTAGAAGGTATGCAATTCGACCGCGGATACGCTTCTCCATACATGATCACGGATACTGA
			TAAGATGGAAGCAGTGCTTGATAATCCGTATATCTTGATTACGGATAAGAAAATCTCTAACATTCAGGAA
			ATCCTTCCTGTGTTAGAGAAAGTTGTACAACAAGGCAAGCCGCTTGTTATCATCGCTGAAGATGTAGAAG
			GCGAAGCACTGGCTACGCTTGTTGTAAATAAATTGCGTGGTACATTTACTGCGGTAGCAGTA
			(SEQ ID NO:108)
A. xylanus	cpn60	ATCC	GCAACAGTTTTAGCACAAGCAATGATTAAGAAGGATTGAAAAACGTTGCTTCTGGACCAAACCCTGTCG
		51415	GTGTTCGCCGTGGAATTGAAAAAGCTGTTGAAGTTGCAGTAGACGAGCTTAGAAAAATTTCACAAACAG
			TTGAAGATAAAGAATCAATCGCTCAAGTTGCAGCTATTTCAGCAAATGACGAAGAAGTAGGTCAATTAA
			TCGCTGAAGCAATGGAGCGCGTTGGTAAGATGGTGTAATTACTGTTGAAGAATCAAGAGGATTCAGCAC
			TGAACTTGAAGTAGTAGAAGGTATGCAATTTGACCGCGGATATACTTCACCATATATGGTATCTGACCA
			AGATAAGATGGAAGCAGTGCTTGAAGATCCATATATTTTAGTAACAGATAAGAAATTAACACATTCAAG
			ATGTATTACCAGTACTTGAGCAAGTTGTACAACAAAGCAAGCCACTATTAATTATTGCTGAAGATGTTG
			AAGGTGAAGCACTTGCAACATTGGTTGTAAACAAACTTCGTGGAACATTTAATGCAGTAGCTGTA
			(SEQ ID NO:109)
V. pantothen-	cpn60	ATCC14576	GCAACTGTATTAGCTCAGTCCATGATTCGTGAAGGTCTTAAACGTAGCATCCGGTGCTAACCCTGTTGG
ticus (=B65			TGTTCGCCGCGGAATCGAAAAGGCTGTTGAAGTAGCAGTAAAAGAACTAAAAATATTTCCAAGTCAATC
B. panthothen-			GAAAGCAAGGAATCTATTGCTCAAGTAGCAGCAGTTTCTTCTGACGATGCAGAAGTTGGTAAGTTAATT
ticus)			TCTGAAGCAATGGAACGTGTTGGTAACGACGGAGTTATTACTATTGAAGAATCAAAAGGTTTCAACACA
			GAGCTAGAAGTAGTTGAAGGTATGCAATTTGACCGTGGATATGCTTCTCCATACATGGTAACAGACCAA
			GACAAAATGGAAGCAGTTTTGGAAAATCCGTACATCCTAATTACGGATAAGAAAATTGGTAACATTCAA
			GAAGTATTACCTATACTTGAACAAGTTGTACAGCAAGGAAAACCTTTATTGATGATTGCTGAGGATGTA
			GAAGGCGAAGCGCTTGCTACATTAGTAGTTAACAAATTGCGTGGAACATTCAATGCAGTAGCTGTA
			(SEQ ID NO:110)
P. popillae	cpn60		GCTACGGTTCTGGCTCAAGCGATGATTCGCGAAGGCTTGAAGAACGTTACGGCTCGCGCGAATCCGATG
			GTCGTTCGCATCAAGGGATCGAGAAAGCAGTGAAANCCGCTGTTGAAGATCTGAAGAAAATTGCGAAGC
			CAATTGAAAACAAGCAAGCATCGCTCAAGTTGCTGCAATCTCTNCCGATGACGAAGAAGTCGGCACATT
			GATCGCAGAAGCAATGGAGAGAGTCGGCAATGACGGTGTAATTACGGTTGAGGAATCCAAAGGCTTCAA
			TACGGAGCTTGAAGTTGTAGAAGGGATGCNATTNGACCNTGGCTNTNTATCTCCGTACATGATCACGGA
			TACGGACAAGATGGAAGCTATCCTCGATACCCCATATATCTTGATCACAGATAAGAAGGTTTCCAACAT
			TCAAGAAATCCTTCCTGTTCTTGAGAAAGTCATTCAACAAGGCAAGCAGCTCCTGATCATCGCTGAGGA
			TGTAGAAGGCGAGCTCAAGCAACCTTGATCTTGAATAAGCTTGCGGACATTCACTTGCGTTGCCGTTA
			(SEQ ID NO:111)
S. pyogenes	cpn60	ATCC19615	GCAACAGTTTTGACACAAGCCATTGTTCATGAAGGACTAAAAAATGTGACAGCAGGTGCTAATCCAATT
			GGTATCCGTCGAGGCATTGAAACAGCAACAGCAACAGCCGTTGAAGCCTTGAAAGCCATTGCTCAACCT
			GTATCTGGCAAGGAAGCTATTGCTCAGGTCGCTGCAGTATCATCACGCTCTGAAAAAGTTGGAGAGTAT
			ATCTCAGAAGCTATGGAGCGTGTGGGCAACGATGGTGTGATTACCATCGAAGAATCTCGAGGTATGGAA
			ACAGAACTTGAAGTG0TTGAAG0CATGCATTTGACCGTGGTTACCTGTCTCAATACATGGTCACAGACA
			ATGAAAAAATGGTTGCAGACCTTGAAAACCCATTTATCTTGATCACGGATAAAAAAGTGTCAAACATCC
			AAGACATTTTGCCACTACTTGAGGAAGTTCTTAAAACCAACCGTCCATTACTCATTATTGCAGATGATG
			TGGATGGTGAGCCCTTCCAACCCTTGTCTTGAACAAGATTCGTGGTACTTTCAATGTGGTTGCTGTC
Escherichia-	cpn60		GCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATG
coli K12			GACCTGAAACGTGGTATCGACAAAGCGGTTACCGCTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCA
			TGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAA
			CTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTG
			CAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAAC
			AAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGACAAGAAAATCTCCAAC
			ATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAA
			AGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGC
			GGTT (SEQ ID NO:113)
Brassica-	cpn60		TCTGTGGTTCTTGCACAAGGTTTTATTGCTGAGGGTGTCAAGGTGGTGCCTGCTGGTGCAAACCCTGTA
napus			TTGATCACTAGAGGCATTGAGAAGACAGCAAAGGCTTTGGTAGCCGAGCTCAAGAAAATGTCTAAGGAG
chloroplast			GTTGAAGACAGTGAGCTTGCAGATGTGGCAGCCGTTAGTGCCGGTAACAATGCAGAAATTGGAAGCATG
beta			ATTGCTGAAGCAATGAGCAGAGTGGGCAGGAAGGGTGTGGTGACACTTGAGGAGGGTAAAAGTGCAGAG
			AACGCTCTCTACGTGGTGGAAGGAATGCAATTTGATCGAGGTTATGTCTCCCCTTACTTTGTGACAGAC
			AGCGAGAAAATGTCAGTTGAGTTCGACAATTGCAAGTTGCTTCTTGTTGACAAGAAAATTACCAATGCA
			AGGGATCTTGTTGGTGTTCTGGAGGATGCAATTAGAGGAGGATACCCAATTTTAATAATTGCGGAAGAC
			ATTGAGCAGGAGGCTTTAGCGACCCTTGTTGTTAACAAGCTTAGAGGCACACTGAAGATTGCAGCTCTC
			(SEQ ID NO:114)

Within this simple system of 16S and cpn60 amplicons from a single species hybridized to amplicon probes of perfect match on the array, the dual backbone prototype was easily able to distinguish three of the four species tested in this assay. H. halophilus gave a strong signal only for its matching 16S and cpn60 probes (FIG. 6). A. xylanus gave a strong signal for the 16S of H. halophilus in addition to its matching 16S probe (FIG. 4). However, the only cpn60 signal came from the probe for A. xylanus. There was no strong signal for its corresponding 16S probe, when V. pantothenticus was hybridized, due to irregularities in the printed DNA spot (FIG. 7). However, two cpn60 probes gave signals—B. pantothenticus and V. pantothenticus. A closer look at these two amplicons revealed that the sequences were identical. Even with an identical sequence, the signal was significantly stronger for the B. pantothenticus than for the V. pantothenticus, which had less DNA deposited on the array (determined by a deoxynucleotidyl terminal transferase assay).

The above three hybridizations were all done with Bacillus-like species that have been reclassified into new genera based on a significant difference from the core Bacillus species. B. amyloliquefaciens (FIG. 5) gave several signals for the 16S probes. It appeared that the B. amyloliquefaciens probe was the strongest, but it was difficult to confirm due to spot irregularities (the mooning effect). When examining the cpn60 probes, signals were obtained from B. subtilis and B. amyloliquefaciens. A closer look at the cpn60 for B. subtilis showed a 6% difference in sequence similarity, which is believed to be too close to discriminate using microarrays. In this case the dual backbone array was able to identify the sample as a Bacillus, and even narrow it down to a pair of species, but it was not able to positively identify it as B. amyloliquefaciens.

From the results obtained above, it was concluded that the optimal hybridization temperature varied between the 16S and cpn60 amplicons, but a compromise at 55° C. at which both types of amplicons hybridized with adequate specificity was appropriate.

By simultaneously assaying for virulence and antimicrobial resistance genes on the same microarray a significant reduction of effort and time were achieved.

The oligonucleotide microarray of the present invention is a powerful tool for the detection of virulence and antimicrobial resistance genes in E. Coli strains.

In accordance with the present invention, it is the first time according to the inventors that two different types of taxonomic sequences (16S and cpn60) have been used together and the results analyzed jointly to obtain corroboration that in some case it is not possible to have otherwise. It is also the first time that antibiotic resistance genes have been used with virulence genes in E. coli on the same array to obtain, in one experiment, information on the nature of the pathogen and how best to treat it. It is also the first time that many variants of the genes probes for virulence are being disclosed to pinpoint the precise type and, in some cases, the target species of the pathogen detected. Thus through a combination of probes, the inventors achieve a better and faster results than previously possible with DNA microarrays of the prior art.

While the invention has been described in connection with specific embodiments thereof, it will be understood that it is capable of further modifications and this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains and as may be applied to the essential features hereinbefore set forth, and as follows in the scope of the appended claims.

Claims

1. An array comprising: a) a substrate; and b) a plurality of nucleic acid probes specifically and simultaneously recognizing the presence of a plurality of different genes, each of said probes being bound to said substrate at a discrete location; said plurality of probes comprising a first probe for detecting a first gene of a species of a microorganism and at least another probe for detecting at least one other gene of said species or of a different species of a microorganism.

2. The array of claim 1, comprising at least two different probes specific for a single gene.

3. The array of claim 2, wherein said array comprises a subarray containing said at least two probes at adjacent discrete locations on said substrate.

4. The array of claim 1, wherein said first probe is specific for a virulence gene or a fragment thereof or a sequence substantially identical thereto, and said at least one other probe is specific for an antibiotic resistance gene.

5. The array of claim 1, wherein said first probe is specific for a variant of a virulence gene or a fragment thereof or a sequence substantially identical thereto, and said at least one other probe is specific for an antibiotic resistance gene, said first probe allowing detection of different types and/or species of microorganism.

6. The array of claim 1, wherein said microorganism is a bacterium.

7. The array of claim 6, wherein said bacterium is of the family Enterobacteriaceae.

8. The array of claim 7, wherein said bacterium is E. coli.

9. The array of claim 4, wherein said virulence gene encodes a polypeptide of a class of proteins selected from the group consisting of toxins, adhesion factors, secretory system proteins, capsule antigens, somatic antigens, flagellar antigens, invasins, autotransporter proteins, and aerobactin system proteins.

10. The array of claim 1, wherein said different genes are selected from the group consisting of Tem, Shv, oxa-1, oxa-7, pse-4, ctx-m, aht(3″)-Ia (aadA1), ant(2″)-Ia (aadB)b, aac(3)-IIa (aacC2), aac(3)-IV, aph(3′)-Ia (aphA1), aph(3′)-IIa (aphA2), tet(A), tet(B), tet(C), tet(D), tet(E), tet(Y), catI, catII, catIII, floR, dhfrI, dhfrV, dhfrVII, dhfrIX, dhfrXIII, dhfrXV, suII, suII, intégron classe 1 3′-CS, vat, vatC, vatD, vatE, vga, vgb, and vgbB,

11. The array of claim 1, wherein said plurality of nucleic acid probes are sequences selected from the group consisting of SEQ ID NO:1 to SEQ ID NO:64, or a fragment thereof, or a sequence having at least 50% identity, preferably at least 70% identity, more preferably having 80% identity and most preferably having 90% identity with said sequences.

12. The array of claim 1, wherein said plurality of different genes are selected from the group consisting of 16S genes, genes encoding heat shock proteins, genes encoding RNA polymerase, genes encoding DNA gyrases, genes encoding lipases, genes encoding cellulases, genes encoding proteases, genes of clinical interest, genes encoding virulence factors, genes encoding growth factors, and genes encoding toxins.

13. The array of claim 1, wherein said first probe is specific for a 16S gene or a fragment thereof or a sequence substantially identical thereto, and said at least one other probe is specific for cpn60 gene.

14. A method of detecting the presence of a microorganism in a sample, said method comprising the steps of: a) contacting the array of claim 1 with a sample nucleic acid of said sample; and b) detecting association of said sample nucleic acid to a probe on said array; wherein association of said sample nucleic acid with said probe is indicative that said sample comprises a microorganism from which the nucleic acid sequence of said probe is derived.

15. The method of claim 14, wherein said method further comprises extracting said sample nucleic acid from said sample prior to contacting said sample nucleic acid with said array.

16. The method of claim 14, wherein said sample is selected from the group consisting of environmental sample, biological sample and food.

17. The method of claim 16 wherein said environmental sample is selected from the group consisting of water, air and soil.

18. The method of claim 16, wherein said biological sample is selected from the group consisting of blood, urine, amniotic fluid, feces, tissues, cells, cell cultures and biological secretions, excretions and discharge.

19. The method of claim 14, wherein said sample is a tissue, body fluid, secretion or excretion from a subject.

20. A method for determining a pathotype of a species of a microorganism in a sample, said method comprising the steps, of: a) contacting the array of claim 1 with a sample nucleic acid of said sample; and b) detecting association of said sample nucleic acid to a probe on said array; wherein association of said sample nucleic acid with said probe is indicative that said sample having a pathotype from which the nucleic acid sequence of said probe is derived.

21. The method of claim 21, further comprising the step of: c) tabulating results for most abundant species based on intensity of the association detected.

22. A method for diagnosing an infection by a microorganism in a subject, said method comprising the steps of: a) contacting the array of claim 1 with a sample nucleic acid of said sample; and b) detecting association of said sample nucleic acid to a probe on said array; wherein association of said sample nucleic acid with said probe is indicative that said sample has been infected by a microorganism from which the nucleic acid sequence of said probe is derived.

23. A kit comprising the array of claim 1 together with instructions for use thereof.

24. The kit of claim 23, wherein said use is for at least one of: (a) detecting the presence of a microorganism in a sample; (b) determining the pathotype of a microorganism in a sample; (c) diagnosing an infection by a microorganism in a subject; (d) diagnosing a condition related to infection by a microorganism, in a subject; (e) characterizing a microbial complex sample or microbial community on a one-time basis; and (f) following the evolution over time of a microbial complex sample or microbial community. This may include comparison between different batches of commercial products based on complex microbial samples, comparison between similar products from different suppliers and monitoring the bacterial composition of commercial products over storage time.