Claims
- 1. An isolated DNA molecule comprising a DNA polynucleotide at least 90% homologous to SEQ ID NO: 3 or fragment, region, or motif thereof.
- 2. A DNA molecule comprising a first DNA polynucleotide at least 90% homologous to SEQ ID NO: 3 or fragment, region, or motif thereof, wherein said first DNA polynucleotide is operably linked to a second DNA polynucleotide heterologous to said first polynucleotide and operably linked to a 3′ transcription termination DNA polynucleotide.
- 3. The DNA molecule of claim 2, wherein said second DNA polynucleotide is transcribed into a RNA molecule that encodes a protein.
- 4. The DNA molecule of claim 2, wherein said second DNA polynucleotide is transcribed into a RNA molecule that does not encode a protein.
- 5. An isolated DNA molecule comprising a DNA polynucleotide set forth in SEQ ID NO: 3 or fragment, region, or motif thereof.
- 6. A DNA molecule comprising a first DNA polynucleotide set forth in SEQ ID NO: 3 or fragment, region, or motif thereof, wherein said first DNA polynucleotide is operably linked to a second DNA polynucleotide heterologous to said first DNA polynucleotide and operably linked to a 3′ transcription termination DNA polynucleotide.
- 7. The DNA molecule of claim 6, wherein said second DNA polynucleotide is transcribed into a RNA molecule that encodes a protein.
- 8. The DNA molecule of claim 6, wherein said second DNA polynucleotide is transcribed into a RNA molecule that does not encode a protein.
- 9. A DNA promoter polynucleotide isolated by a method comprising the steps of: (i) preparation of plant genomic DNA; and (ii) preparation of a DNA polynucleotide primer having sequence homology to a length of SEQ ID NO: 12; and (iii) mixing said DNA polynucleotide primer and genomic DNA with a second DNA polynucleotide primer not homologous to SEQ ID NO: 12; and (iv) subjecting said mixture to a PCR condition that provides an amplicon; and (v) purifying said amplicon; (vi) inserting said amplicon into an expression construct; and (vii) testing said expression construct for expression of a reporter molecule.
- 10. A DNA promoter polynucleotide isolated by a method comprising the steps of: (i) preparation of plant genomic DNA; and (ii) preparation of a mixture of degenerate DNA polynucleotide primers to a length of the translation product of SEQ ID NO: 12; and (iii) mixing said degenerate DNA polynucleotide primers and genomic DNA with a second DNA polynucleotide primer not homologous to SEQ ID NO: 12; and (iv) subjecting said mixture to a PCR condition that provides an amplicon; and (v) purifying said amplicon; (vi) inserting said amplicon into an expression construct; and (vii) testing said expression construct for expression of a reporter molecule.
- 11. A DNA promoter polynucleotide isolated by a method comprising the steps of: (i) preparation of plant genomic DNA; and (ii) preparation of a DNA polynucleotide primer having sequence homology to a 5′ length of SEQ ID NO: 3; and (iii) mixing said DNA polynucleotide primer and genomic DNA with a second DNA polynucleotide primer having sequence homology to a 5′ length of SEQ ID NO: 12; and (iv) subjecting said mixture to a PCR condition that provides an amplicon; and (v) purifying said amplicon; (vi) inserting said amplicon into an expression construct; and (vii) testing said expression construct for expression of a reporter molecule.
- 12. A transgenic plant made by a method comprising the steps of: (i) introducing into the genome of a plant cell a DNA molecule comprising a first DNA polynucleotide at least 90% homologous to SEQ ID NO: 3 or motif thereof, wherein said first DNA polynucleotide is operably linked to a second DNA polynucleotide heterologous to said first polynucleotide and operably linked to a 3′ transcription termination DNA polynucleotide; and (ii) selecting said transgenic plant cell; and (iii) regenerating said transgenic plant cell into a transgenic plant.
- 13. The transgenic plant of claim 12, wherein said DNA molecule confers disease resistance to said transgenic plant.
- 14. The transgenic plant of claim 12, wherein said DNA molecule confers enhanced root growth to said transgenic plant.
- 15. The transgenic plant of claim 12, wherein said DNA molecule confers insect resistance to said transgenic plant.
- 16. The transgenic plant of claim 12, wherein said DNA molecule confers herbicide tolerance to said transgenic plant.
- 17. The transgenic plant of claim 12, wherein said DNA molecule confers stress tolerance to said transgenic plant.
- 18. A seed of said transgenic plant of claim 12.
- 19. The isolated DNA molecule of claim 1, wherein said polynucleotide is at least 90% homologous to SEQ ID NO: 3.
- 20. The isolated DNA molecule of claim 1, wherein said polynucleotide is set forth in SEQ ID NO: 3.
Parent Case Info
[0001] This application claims priority to an earlier filed U.S. Provisional application No. 60/310,370, filed Aug. 6, 2001.
Provisional Applications (1)
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Number |
Date |
Country |
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60310370 |
Aug 2001 |
US |