Claims
- 1. A method for purifying DNA comprising the steps of:
- (a) contacting a suspension containing DNA with a silicon-containing material which exhibits sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the material with water, said silicon-containing material selected from the group consisting of boron silicate prepared by reacting SiCl.sub.4 with about 0.09 to about 1.5 equivalents of BCl.sub.3 in the presence of water, aluminum silicate prepared by reacting SiCl.sub.4 with about 0.1 to about 0.5 equivalents of AlCl.sub.3 in the presence of water, phosphosilicate prepared by reacting SiCl.sub.4 with about 0.1 to about 5 equivalents of PCl.sub.3 in the presence of water, silica carbonyl prepared by reacting SiCl.sub.4 with about 0.1 to about 1.5 equivalents of COCl.sub.2 in the presence of water, silica sulfonyl prepared by reacting SiCl.sub.4 with about 0.1 to about 1.5 equivalents of SOCl.sub.2 in the presence of water, and silica phosphonyl prepared by reacting SiCl.sub.4 with about 0.1 to about 2 equivalents of POCl.sub.3 in the presence of water under conditions suitable to bind DNA to said material;
- (b) washing said material having bound DNA; and
- (c) eluting the bound DNA from said material.
- 2. The method of claim 1 wherein the silicon-containing material is a phosphosilicate prepared by reacting SiCl.sub.4 with about 0.1 to about 5 equivalents of PCl.sub.3 in the presence of water.
- 3. The method of claim 1 wherein the silicon-containing material is a phosphosilicate prepared by reacting SiCl.sub.4 with about 1 to about 5 equivalents of PCl.sub.3 in the presence of water.
Parent Case Info
This is a division of application Ser. No. 08/127,403, filed Sep. 27, 1993, now U.S. Pat. No. 5,503,816.
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Aug 1994 |
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Divisions (1)
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Number |
Date |
Country |
Parent |
127403 |
Sep 1993 |
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