Yang et al., "Identification of the Pleiotropic sacQ Gene of Bacillus subis. J. Bacteriol., vol. 166, No. 1 (Apr. 1986), 113-119. |
Tomioka et al., "Cloning, sequencing, and some properties of a novel Bacillus amyloliquefaciens gene involved in the increase of extracellular protease activities," Journal of Biotechnology, 3, (1985), 85-96. |
Takeichi et al., "Cloning of Bacillus subtilis .alpha.-Amylase Structural Gene in Plasmid pUB110," Agric. Biol. Chem., vol. 47, No. 1, (1983), 159-161. |
Marmur, J., "A Procedure for the Isolation of Deoxyribonucleic Acid from Microorganisms," J. Mol. Biol., 3, (1961), 208-218. |
Hagihara et al., "Crystalline Bacterial Proteinase," The Journal of Biochemistry, vol. 45, No. 3, (1958), 185-194. |
Hidetsngu Fuwa, "A New Method for Microdetermination of Amylase Activity By The Use of Amylose As The Substrate," The Journal of Biochemistry, vol. 41, No. 1, (1954), 583-603. |
Y. Takagi, "Experimental Methods for Gene Manipulation," p. 139. |
Takegi et al., "Nucleotide Sequence and Promoter Region for the Neutral Protease Gene from Bacillus stearothermophilus, vol. 163, No. 3, (Sep. 1985), 824-831. |
Anagnostopoulos et al., "Requirements for Transformation of Bacillus subtilis," J. Bacteriol., 81, (1961), 741-746. |
Fuji et al., "Molecular Cloning of a Thermostable Neutral Protease Gene . . . ", J. Bacteriol., vol. 154, No. 2, (May 1983), 831-837. |
Chang et al., "Cloning and expression of heterologous genes in Bacillus subtilis, (NSC Symp. Ser, 4, (1982), 254-62) Chemical Abstracts 100:46295m. |
Blobel et al., "Transfer of Proteins Across Membranes," The Journal of Cell Biology, 67, (1975), 835-851. |
Williams et al., "Expressions of Escherichia coli trp . . . " Gene, 16, (1981). |
Saito et al., "Preparation of Transforming Deoxyribonucleic Acid by Phenol Treatment," Biochem. Biophys. Acta., 72, (1963), 619-629. |
Mandel et al., "Calcium-dependent Bacteriophage DNA Infection," J. Mol. Biol., 53, (1970), 159-162. |
Horinouchi, "Expression of Information in Gram-positive Bacteria," Tanpakushitu-Kakusan-Koso 28, (1983), 1468-1478. |
Moran, Jr. et al., "Nucleotide Sequences that Signal the Initiation of Transcription and Translation in Bacillus subtilis," Mol. Gen. Genet., 186, (1982), 339-346. |
David A. Dubnau, et., The Molecular Biology of the Bacilli (New York: Academic Press, 1982), I, pp. 331 and 332. |
Goldfarb et al., "Expression of Tn9-derived Chloramphenicol . . . ", Nature, vol. 293, No. 5830, (Sep. 1981), pp. 309-311. |
Chang et al., "Expression of Eukaryotic Genes in B. subtilis Using Signals of penP," Molecular Cloning and Gene Regulation in Bacilli, Ganesan et al., eds., (New York: Academic Press, 1982), pp. 159-169. |
T. Maniatis, E. Fritsch, J. Sambrook, Molecular Cloning, A Laboratory Manual, (Cold Spring Harbor, NY: Cold Spring Harbor Lab., 1982), pp. 3-5, 51, 52, 270-2. |
Honjo et al., "Cloning and expression of the gene for neutral protease of bacillus amyloliquefaciens . . . ", J. Biotechnol., vol. 1, (1984), 265-277. |
Vasantha et al., "Genes for Alkaline Protease and Neutral Protease from Bacillus amyloliquefaciens . . . " J. Bacteriol., vol. 159, No. 3, (Sep. 1984), 811-819. |
Yang et al., "Cloning of the Neutral Protease Gene of Bacillus subtilis . . . " J. Bacteriol., vol. 160, No. 1, (Oct. 1984), 15-21. |
Uehara et al., "Regulation of Neutral Protease Productivity in Bacillus subtilis, Transformation of High Protease Productivity," J. Bacterol., 119, (1974), 82-81. |
Palva et al., "Nucleotide sequence of the promoter and NH.sub.2 -terminal signal peptide region of the .alpha.-amylase gene from Bacillus amyloliquefaciens," Gene, 15, (1981), 43-51. |
"Experimental Agricultural Chemistry", pp. 284-285, Asakura Shuppan (1978) with translation. |
McLaughlin et al., "Unique Features in the Ribosome Binding Site Sequence of the Gram-positive Staphylococcus aureus .beta.-Lactamase Gene", The Journal of Biological Chemistry, vol. 256, (Nov. 10, 1981), pp. 11283-11291. |
Gryczan, T. J. et al., J. Bacteriol., 132, (1978), pp. 317 to 329. |
Chang, S. et al., Mol. Gen. Genet., 168, (1978), pp. 111 to 115. |
Yamaguchi, K. et al., J. Bacteriol., 119, (1974), pp. 416 to 424. |
Birnboim, H. C. et al., Nucleic Acids Res., 7, (1979), pp. 1513 to 1523. |
Maxam, H. C. et al., Proc. Natl. Acad. Sci. USA, 7, (1977), pp. 560 to 564. |
Ishikawa, E., (ed.), Enzyme Immunoassay, (1981), pp. 67 to 81. |
I. R. Lehman, "T4 DNA Polymerase", Method in Enzymology, vol. 29, (1978). |
Biochemical Information II, Boehringer Mannheim, pp. 28-30. |
Hayashi et al., J. Bio. Chem., vol. 259, No. 16 (1984), pp. 10448 to 10454. |
Mezes et al., J. Bio. Chem., vol. 258, No. 18 (1983), pp. 11211 to 12218. |
Schaeffer et al., "Contribution a l'etude gnetique de la sporogenese bacterienne," Comptes Rendus, vol. 251, No. 25 (Dec. 19, 1960), pp. 3125-3127. |
Saito et al., "DNA-Mediated Transformation in Bacillus subtilis With Special Reference to the Method for Preparing Competent Cells", Journal of General Applied Microbiology, vol. 1, No. 4 (1961), pp. 243-252. |
Millet, J., "Characterization of Proteinases Excreted by Bacillus subtilis Marburg Strain during Sporulation", Journal of Applied Bacteriology, vol. 33, (1970), pp. 207-219. |
Laemmli, U. K., "Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4", Nature, vol. 227 (Aug. 15, 1970), pp. 680-685. |
Higerd et al., "Hyperprotease-Producing Mutants of Bacillus subtilis", Journal of Bacteriology, vol. 112, No. 2 (Nov. 1972), pp. 1026-1028. |
Hageman et al., "Effects of Mutational Loss of Specific Intracellular Proteases on the Sporulation of Bacillus subtilis," Journal of Bacteriology, vol. 114, No. 2 (May 1973), pp. 612-617. |
Kunst et al., "Pleiotropic mutations affecting sporulation conditions and the syntheses of extracellular enzymes in Bacillus subtilis 168", Biochime, vol. 56 (1974), pp. 1481-1489. |
Chemical Abstract 84:27437u of Levy et al., "Evidence of homologous relation between thermolysin and neutral protease A of Bacillus subtilis", Proceedings of the National Academy of Sciences, vol. 72, No. 11 (1975), pp. 4341-4345. |
Yoneda et al., "Mutation of Bacillus subtilis Causing Hyperproduction of .alpha.-amylase and Protease, and Its Synergistic Effect," Journal of Bacteriology, vol. 124, No. 1 (Oct. 1975), pp. 48-54. |
Steinmetz et al., "Mapping of Mutations Affecting Synthesis of Exocellular Enzymes in Bacillus subtilis," Molecular & General Genetics, vol. 148 (1976), pp. 281-285. |
Bolivar et al., "Plasmids of Escherichia coli as Cloning Vectors," in: Methods in Enzymology (1979 ed.), vol. 68, pp. 245-267. |
Contente et al., "Characterization of Plasmid Transformation in Bacillus subtilis: Kinetic Properties and the Effect of DNA Conformation", Molecular & General Genetics, vol. 167 (1979), pp. 251-258. |
Uehara et al., "Thermosensitive, Extracellular Neutral Proteases in Bacillus subtilis: Isolation, Characterization, and Genetics", Journal of Bacteriology, vol. 139, No. 2 (Aug. 1979), pp. 583-590. |
Goeddel et al., "Direct Expression in Escherichia coli of a DNA sequence coding for human growth hormone, Nature, vol. 281 (Oct. 11, 1979), pp. 544-548. |
Gryczan et al., "Molecular Cloning of Heterologous Chromosomal DNA by Recombination between a Plasmid Vector and a Homologous Resident Plasmid in Bacillus subtilis", Molecular & General Genetics, vol. 177, No. 3 (1980), pp. 459-467. |
Mantsala et al., "Extracellular and Membrane-Bound Proteases from Bacillus subtilis," Journal of Bacteriology, vol. 141, No. 2, pp. 493-501. |
Tanpakushitsu-Kakusan-Koso, vol. 26, No. 13 (1981), pp. 2043-2046. |
Tomioka et al., "Abstracts of Papers", Annual Meeting of the Agricultural Society of Japan, 1983, p. 33. |
Takeichi et al., "Cloning of Bacillus subtilis .alpha.-Amylase Structural Gene in Plasmid pUB110", Agricultural and Biological Chemistry, vol. 47, No. 1 (Jan. 1983). |
Palva et al., "Secretion of interferon by Bacillus subtilis", Gene, vol. 22 (1983), pp. 229-235. |
Stahl et al., "Replacement of the Bacillus subtilis Subtilisin Structural Gene with an In Vitro-Derived Deletion Mutation", Journal of Bacteriology, vol. 158, No. 2 (May 1984), pp. 411-418. |
Kawamura et al., "Construction of a Bacillus subtilis Double Mutant Deficient in Extracellular Alkaline and Neutral Proteases", Journal of Bacteriology, vol. 160, No. 1 (Oct. 1984), pp. 442-444. |
Manabe et al., "N-Terminal Amino Acid Sequences of Neutral Proteases from Bacillus amyloliquefaciens and Bacillus subtilis: Identification of a Neutral Protease Gene Cloned in Bacillus subtilis", Agricultural Biological Chemistry, vol. 49, No. 8 (1985), pp. 2261-2267. |
Shimada et al., "The Nucleotide Sequence and Some Properties of the Neutral Protease Gene of Bacillus amyloliquefaciens," Journal of Biotechnology, vol. 2 (1985), pp. 57-85. |
Honjo et al., "Construction of the secretion vector containing the prepro-structure coding region of Bacillus amyloliquefaciens neutral protease gene and secretion of Bacillus subtilis .alpha.-amylase and human interferon-beta in Bacillus subtilis", Journal of Biotechnology, vol. 3, (1985), pp. 73-84. |
Ulmanen et al., "Transcription and Translation of Foreign Genes in Bacillus subtilis by the Aid of a Secretion Vector", Journal of Bacteriology, vol. 162, No. 1 (Apr. 1985), pp. 176-182. |
Yamane et al., "Bacillus subtilis Secretion Vectors for Proteins and Oligopeptides Constructed from B. subtilis .alpha.-Amylase Genes" in: Molecular Biology of Microbial Differentiation, American Society for Microbiology (1985), pp. 117-123. |
Schein et al., "Secretion of Mature IFN--.alpha.2 and Accumulation of Uncleaved Precursor by Bacillus subtilis Transformed with a Hybrid .alpha.-Amylase Signal Sequence-IFN-.alpha.2 Gene", BIO/TECHNOLOGY, vol. 4 (Aug. 1986), pp. 719-725. |