Drug eluting medical devices having porous layers

Abstract

In accordance with an aspect of the invention, implantable or insertable medical devices are provided that comprise (a) a substrate and (b) a porous layer comprising close packed spherical pores disposed over the substrate. The porous layer may also comprise a therapeutic agent. In another aspect, the present invention provides methods of forming implantable or insertable medical devices. These methods comprise forming a predecessor structure that comprises (i) a substrate over which is disposed (ii) an assembly of microspheres. This assembly of microspheres is then used as a template for the formation of a porous layer, which may be subsequently loaded with a therapeutic agent.

Description

FIELD OF THE INVENTION

This invention relates to medical devices, and more particularly to drug eluting medical devices having porous layers.

BACKGROUND OF THE INVENTION

The in vivo delivery of therapeutic agents within the body of a patient is common in the practice of modern medicine. In vivo delivery of therapeutic agents is often implemented using medical devices that may be temporarily or permanently placed at a target site within the body. These medical devices can be maintained, as required, at their target sites for short or prolonged periods of time, delivering biologically active agents at the target site.

In accordance with certain delivery strategies, a therapeutic agent is provided within or beneath a biostable polymeric layer that is associated with a medical device. Once the medical device is placed at the desired location within a patient, the therapeutic agent is released from the medical device with a profile that is dependent, for example, upon the nature of the therapeutic agent and of the polymeric layer, among other factors.

Examples of such devices include drug eluting coronary stents, which are commercially available from Boston Scientific Corp. (TAXUS), Johnson & Johnson (CYPHER), and others. For example, the TAXUS stent contains a non-porous polymeric coating consisting of an antiproliferative drug (paclitaxel) within a biostable polymer matrix. The drug diffuses out of the coating over time. Due to the relatively low permeability of paclitaxel within the polymer matrix and due to the fact that the polymer matrix is biostable, a residual amount of the drug remains in the device beyond its period of usefulness (e.g., after the coating is overgrown with cells). Moreover, smooth surfaces by their nature do not allow for cell in-growth. Furthermore, smooth surfaces commonly exhibit inferior cell adhesion and growth relative to textured surfaces. For example, feature sizes less than 100 nm are believed to promote adhesion of proteins such as fibronectin, laminin, and/or vitronectin to the surface, and to provide a conformation for these proteins that better exposes amino acid sequences such as RGD and YGSIR which enhance endothelial cell binding. See, e.g., Standard handbook of biomedical engineering and design, Myer Kutz, Ed., 2003 ISBN 0-07-135637-1, p. 16.13. Moreover, small surface features are associated with an increase in surface energy, which is believed to increases cell adhesion. See, e.g., J. Y. Lim et al., J. Biomed Mater. Res. (2004) 68A(3): 504-512. In this regard, submicron topography, including pores, fibers, and elevations in the sub-100 nm range, has been observed for the basement membrane of the aortic valve endothelium as well as for other basement membrane materials. See R. G. Flemming et al., Biomaterials 20 (1999) 573-588, S. Brody et al., Tissue Eng. 2006 Feb; 12(2): 413-421, and S. L. Goodman et al., Biomaterials 1996; 17: 2087-95. Goodman et al. employed polymer casting to replicate the topographical features of the subendothelial extracellular matrix surface of denuded and distended blood vessels, and they found that endothelial cells grown on such materials spread faster and appeared more like cells in their native arteries than did cells grown on untextured surfaces. See also F. L. Yap et al., “Protein and cell micropatterning and its integration with micro/nanoparticles assembly,” Biosensors and Bioelectronics 22 (2007) 775-778.

SUMMARY OF THE INVENTION

In accordance with an aspect of the invention, implantable or insertable medical devices are provided that comprise (a) a substrate and (b) a porous layer comprising close packed spherical pores disposed over the substrate. The porous layer may also comprise a therapeutic agent, for example, within the pores, within the material that forms the porous layer, conjugated to the material that forms the porous layer, and so forth.

In another aspect, the present invention provides methods of forming implantable or insertable medical devices. These methods comprise forming a predecessor structure that comprises (i) a substrate over which is disposed (ii) an assembly of microspheres. This assembly of microspheres is then used as a template for the formation of a porous layer, which may be subsequently loaded with a therapeutic agent.

In another aspect, the present invention provides implantable or insertable medical devices, which comprise a medical device substrate and a therapeutic-agent-loaded, porous coating, which is formed by the above methods.

Depending on the embodiment that is practiced, advantages of the present invention may include one or more of the following, among others: (a) reduced or selective retention of therapeutic agent(s), (b) improved cell adhesion, (c) improved cell proliferation, (d) improved cell in-growth, (e) prevention of contact between bodily tissue and bioadverse substrates, if present, and (f) prevention of fragmentation of biodegradable substrates, if present.

These and other embodiments and advantages of the present invention will become immediately apparent to those of ordinary skill in the art upon review of the Detailed Description and Claims to follow.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A-1C are microscopic images of porous surfaces generated by electron beam irradiation in accordance with the prior art.

FIG. 2 is a schematic illustration of an electrochemical apparatus for electrophoretic deposition of colloidal crystals on the luminal surface of a stent (viewed along the axis of the stent), in accordance with an embodiment of the present invention.

DETAILED DESCRIPTION

As noted above, the present invention, in one aspect, provides implantable or insertable medical devices that comprise (a) a substrate and (b) a porous layer (e.g., a ceramic, polymeric or polymeric-ceramic hybrid layer, among others) disposed over the substrate, which porous layer comprises close packed spherical pores (e.g., hexagonally close packed spherical pores). The porous layer may also comprise a therapeutic agent, for example, within the pores, within the material that forms the porous layer, conjugated to the material that forms the porous layer, and so forth. In some embodiments, the pores are interconnected, whereas in other embodiments, the pores are not.

In another aspect, the present invention provides methods of forming implantable or insertable medical devices. These methods comprise forming a predecessor structure that comprises (i) a substrate over which is disposed (ii) an assembly of microspheres (e.g., a colloidal crystal, etc.). In some embodiments, the microspheres will comprise a therapeutic agent (e.g., within the microspheres, within a layer that is coated on the microspheres, conjugated to the surface of the microspheres, etc.), whereas in other embodiments, the microspheres will not comprise a therapeutic agent. These microspheres are then used as templates for the formation of a porous layer, which in some instances may be subsequently loaded with a therapeutic agent.

For example, in some embodiments, the microspheres contain one or more types of radiation degradable polymers, in which case the predecessor structure may be irradiated with electrons such that a porous layer is created from the microsphere assembly itself. In other embodiments, the interstices (i.e., voids or vacant spaces) between the microspheres are infiltrated with a material in fluid form (e.g., liquid form, particulate form, particles suspended in a liquid, etc.), followed by solidification of the material. The microspheres are then removed to yield a porous layer.

In each of the above techniques, the ultimate pore size within the porous layers will be dictated by the size of the microspheres.

In another aspect, the present invention provides implantable or insertable medical devices, which comprise a medical device substrate and a therapeutic-agent-loaded, porous coating made by the above techniques.

As used herein, “microspheres” are small spheres, for example, spheres that are 100 microns (μm) or less in diameter, ranging, for example, from 100 μm or more to 30 μm 10 μm to 5 μm to 3 μm to 1 μm to 300 nm to 100 nm to 30 nm to 10 nm or less. As used herein, a “nanopore” is a pore having a width that does not exceed 1 micron in width. As used herein, “micropores” are smaller than 2 nm in width, “mesopores” range from 2 to 50 nm in width, and “macropores” are larger than 50 nm in width. As used herein a “porous” layer is a layer that contains pores. A “nanoporous layer” is a layer that contains nanopores; a “macroporous layer” is a layer that contains macropores; and so forth.

Spherical pores in the porous layers of the present invention typically range, for example, from 100 μm or more to 30 μm to 10 μm to 5 μm to 3 μm to 1 μm to 300 nm to 100 nm to 30 nm to 10 nm or less in diameter.

As used herein, a “polymeric material ” is a material that contains one or more types of polymers, for example, from 50 wt % or less to 75 wt % to 90 wt % to 95 wt % to 97.5 wt % to 99 wt % or more polymers.

As used herein, a “ceramic material” is a material that contains one or more ceramic species, for example, from 50 wt % or less to 75 wt % to 90 wt % to 95 wt % to 97.5 wt % to 99 wt % or more ceramic species.

As used herein, a “metallic material” is a material that contains one or more metals, for example, from 50 wt % or less to 75 wt % to 90 wt % to 95 wt % to 97.5 wt % to 99 wt % or more metals.

As used herein a “layer” of a given material is a region of that material whose thickness is small compared to both its length and width. A layer need not be planar, for example, taking on the contours of an underlying substrate. A layer can be discontinuous (e.g., patterned). A layer can include multiple sub-layers.

As used herein, “polymers” are molecules containing multiple copies of one or more constitutional units, commonly referred to as monomers. Polymers may take on a number of configurations including linear, cyclic and branched configurations, among others. As used herein, “homopolymers” are polymers that contain multiple copies of a single constitutional unit. “Copolymers” are polymers that contain multiple copies of at least two dissimilar constitutional units, examples of which include random, statistical, gradient, periodic (e.g., alternating) and block copolymers. As used herein, the term “monomers” may refer to free monomers and to those that are incorporated into polymers, with the distinction being clear from the context in which the term is used.

As used herein, “radiation degradable polymers” are polymers whose backbones break down into lower molecular weight fragments upon electron beam irradiation. Examples of radiation degradable polymers include homopolymers and copolymers comprising methyl methacrylate, 2,2,2-trifluoroethyl-chloroacrylate, or butene-1-sulfone, among others, for example, poly(methyl methacrylate) (PMMA), poly(methyl methacrylate-co-butyl acrylates), including poly(methyl methacrylate-b-butyl acrylate-b-methyl methacrylate), poly(2,2,2-trifluoroethyl-chloroacrylate), poly(butene-1-sulfone) and poly(chloromethacrylate-co-methylstyrene). Of these, poly(methyl methacrylate), poly(2,2,2-trifluoroethyl-chloroacrylate), poly(butene-1-sulfone) and poly(chloromethacrylate-co-methylstyrene) are commonly used as a positive e-beam resist materials.

As used herein, “monodisperse” microspheres are a group of microspheres which are of substantially the same size, for example, having a size distribution such that at least 95% of the spheres have diameters that are within 10% of one another.

The underlying substrate is not limited to any particular material. For example, substrate materials can be selected from a variety of polymeric, ceramic and metallic materials, as well as combinations two or more of the same (e.g., hybrid materials), among various other materials. Specific examples of ceramic substrate materials may be selected, for example, from suitable materials containing one or more of the following: metal oxides, including aluminum oxides and transition metal oxides (e.g., oxides of titanium, zirconium, hafnium, tantalum, molybdenum, tungsten, rhenium, and iridium); silicon; silicon-based ceramics, such as those containing silicon nitrides, silicon carbides and silicon oxides (sometimes referred to as glass ceramics); calcium phosphate ceramics (e.g., hydroxyapatite); carbon and carbon-based, ceramic-like materials such as carbon nitrides, among many others, that will be recognized by those of ordinary skill in the art after reading this disclosure.

Specific examples of metallic substrate materials may be selected, for example, from suitable substantially pure metals (e.g., biostable metals such as gold, platinum, palladium, iridium, osmium, rhodium, titanium, tantalum, tungsten, and ruthenium, and bioresorbable metals such as magnesium and iron), metal alloys comprising iron and chromium (e.g., stainless steels, including platinum-enriched radiopaque stainless steel), alloys comprising nickel and titanium (e.g., Nitinol), alloys comprising cobalt and chromium, including alloys that comprise cobalt, chromium and iron (e.g., elgiloy alloys), alloys comprising nickel, cobalt and chromium (e.g., MP 35N) and alloys comprising cobalt, chromium, tungsten and nickel (e.g., L605), alloys comprising nickel and chromium (e.g., inconel alloys), and bioabsorbable metal alloys such as magnesium and iron alloys (including their combinations with Ce, Ca, Zn, Zr, Li, etc.), among many others, that will be recognized by those of ordinary skill in the art after reading this disclosure.

Examples of polymeric substrate materials include those that contain one or more suitable biostable or biodegradable polymers, and may be selected, for example, from suitable materials containing one or more of the following: polycarboxylic acid polymers and copolymers including polyacrylic acids; acetal polymers and copolymers; acrylate and methacrylate polymers and copolymers (e.g., n-butyl methacrylate); cellulosic polymers and copolymers, including cellulose acetates, cellulose nitrates, cellulose propionates, cellulose acetate butyrates, cellophanes, rayons, rayon triacetates, and cellulose ethers such as carboxymethyl celluloses and hydroxyalkyl celluloses; polyoxymethylene polymers and copolymers; polyimide polymers and copolymers such as polyether block imides, polyamidimides, polyesterimides, and polyetherimides; polysulfone polymers and copolymers including polyarylsulfones and polyethersulfones; polyamide polymers and copolymers including nylon 6,6, nylon 12, polyether-block co-polyamide polymers (e.g., Pebax® resins), polycaprolactams and polyacrylamides; resins including alkyd resins, phenolic resins, urea resins, melamine resins, epoxy resins, allyl resins and epoxide resins; polycarbonates; polyacrylonitriles; polyvinylpyrrolidones (cross-linked and otherwise); polymers and copolymers of vinyl monomers including polyvinyl alcohols, polyvinyl halides such as polyvinyl chlorides, ethylene-vinylacetate copolymers (EVA), polyvinylidene chlorides, polyvinyl ethers such as polyvinyl methyl ethers, vinyl aromatic polymers and copolymers such as polystyrenes, styrene-maleic anhydride copolymers, vinyl aromatic-hydrocarbon copolymers including styrene-butadiene copolymers, styrene-ethylene-butylene copolymers (e.g., a polystyrene-polyethylene/butylene-polystyrene (SEBS) copolymer, available as Kraton® G series polymers), styrene-isoprene copolymers (e.g., polystyrene-polyisoprene-polystyrene), acrylonitrile-styrene copolymers, acrylonitrile-butadiene-styrene copolymers, styrene-butadiene copolymers and styrene-isobutylene copolymers (e.g., polyisobutylene-polystyrene block copolymers such as SIBS), polyvinyl ketones, polyvinylcarbazoles, and polyvinyl esters such as polyvinyl acetates; polybenzimidazoles; ionomers; polyalkyl oxide polymers and copolymers including polyethylene oxides (PEO); polyesters including polyethylene terephthalates, polybutylene terephthalates and aliphatic polyesters such as polymers and copolymers of lactide (which includes lactic acid as well as d-,l- and meso lactide), epsilon-caprolactone, glycolide (including glycolic acid), hydroxybutyrate, hydroxyvalerate, para-dioxanone, trimethylene carbonate (and its alkyl derivatives), 1,4-dioxepan-2-one, 1,5-dioxepan-2-one, and 6,6-dimethyl-1,4-dioxan-2-one (a copolymer of polylactic acid and polycaprolactone is one specific example); polyether polymers and copolymers including polyarylethers such as polyphenylene ethers, polyether ketones, polyether ether ketones; polyphenylene sulfides; polyisocyanates; polyolefin polymers and copolymers, including polyalkylenes such as polypropylenes, polyethylenes (low and high density, low and high molecular weight), polybutylenes (such as polybut-1-ene and polyisobutylene), polyolefin elastomers (e.g., santoprene), ethylene propylene diene monomer (EPDM) rubbers, poly-4-methyl-pen-1-enes, ethylene-alpha-olefin copolymers, ethylene-methyl methacrylate copolymers and ethylene-vinyl acetate copolymers; fluorinated polymers and copolymers, including polytetrafluoroethylenes (PTFE), poly(tetrafluoroethylene-co-hexafluoropropene) (FEP), modified ethylene-tetrafluoroethylene copolymers (ETFE), and polyvinylidene fluorides (PVDF); silicone polymers and copolymers; polyurethanes; p-xylylene polymers; polyiminocarbonates; copoly(ether-esters) such as polyethylene oxide-polylactic acid copolymers; polyphosphazines; polyalkylene oxalates; polyoxaamides and polyoxaesters (including those containing amines and/or amido groups); polyorthoesters; biopolymers, such as polypeptides, proteins, polysaccharides and fatty acids (and esters thereof), including fibrin, fibrinogen, collagen, elastin, chitosan, gelatin, starch, and glycosaminoglycans such as hyaluronic acid; as well as blends and further copolymers of the above, among many others that will be recognized by those of ordinary skill in the art after reading this disclosure.

Examples of medical devices benefiting from the present invention include implantable or insertable medical devices, for example, selected from stents (including coronary vascular stents, peripheral vascular stents, cerebral, urethral, ureteral, biliary, tracheal, gastrointestinal and esophageal stents), stent coverings, stent grafts, vascular grafts, catheters (e.g., urological or vascular catheters such as balloon catheters and various central venous catheters), guide wires, balloons, filters (e.g., vena cava filters and mesh filters for distil protection devices), abdominal aortic aneurysm (AAA) devices (e.g., AAA stents, AAA grafts), vascular access ports, dialysis ports, embolization devices including cerebral aneurysm filler coils (including Guglilmi detachable coils and metal coils), embolic agents, hermetic sealants, septal defect closure devices, myocardial plugs, patches, pacemakers, lead coatings including coatings for pacemaker leads, defibrillation leads, and coils, ventricular assist devices including left ventricular assist hearts and pumps, total artificial hearts, shunts, valves including heart valves and vascular valves, anastomosis clips and rings, cochlear implants, tissue bulking devices, and tissue engineering scaffolds for cartilage, bone, skin and other in vivo tissue regeneration, sutures, suture anchors, tissue staples and ligating clips at surgical sites, cannulae, metal wire ligatures, urethral slings, hernia “meshes”, artificial ligaments, orthopedic prosthesis and dental implants, among others.

The medical devices of the present invention thus include, for example, implantable and insertable medical devices that are used for systemic treatment, as well as those that are used for the localized treatment of any mammalian tissue or organ. Non-limiting examples are tumors; organs including the heart, coronary and peripheral vascular system (referred to overall as “the vasculature”), the urogenital system, including kidneys, bladder, urethra, ureters, prostate, vagina, uterus and ovaries, eyes, ears, spine, nervous system, lungs, trachea, esophagus, intestines, stomach, brain, liver and pancreas, skeletal muscle, smooth muscle, breast, dermal tissue, cartilage, tooth and bone.

As noted above, in accordance with an aspect of the invention, a predecessor structure is formed that comprises an assembly of monodisperse polymeric microspheres disposed upon a substrate. These microspheres are then used as templates for the formation of a porous layer.

In certain embodiments, the microspheres are colloids (also referred to as “colloidal spheres”). As used herein, “colloids” are solid particles, which can be dispersed in a liquid medium such that they do not settle by gravity. Such dispersions are referred to as “colloidal dispersions.” Colloids are usually less than about 2 microns in diameter.

Monodisperse colloidal spheres can be assembled into two-dimensional arrays (i.e., assemblies that are one microsphere thick) and three-dimensional arrays (i.e., assemblies that are two or more microspheres in thickness, e.g., ranging from 2 to 5 to 10 to 20 to 50 to 100 or more microspheres in thickness). Polydisperse microspheres may also be employed as well. Also, two or more layers of monodisperse microspheres of different size may be employed, for example, for purposes of forming a mesoporous layer over a macroporous layer, or vice versa.

For example, (a) a two-dimensional array of colloidal spheres may be first formed at an air-liquid interface and this array can be subsequently transferred onto the surface of a medical device substrate, (b) a dispersion of colloidal spheres may be spread onto the surface of a substrate and the solvent evaporated slowly under controlled conditions such that they close pack into a two-dimensional array, or (c) colloidal spheres may be assembled on a conductive medical device substrate in a closed packed two-dimensional array via electrophoretic deposition. For further information on these techniques, see, e.g., Y. Xia et al., “Monodispersed Colloidal Spheres: Old Materials with New Applications,” Adv. Mater., 2000, 12(10), 693-713 and the references cited therein.

Three-dimensional colloidal sphere arrays (sometimes referred to as “colloid crystals”) may be formed, for example, (a) via repulsive electrostatic interactions, in which highly charged colloidal spheres are spontaneously organized into crystalline colloids under suitable conditions or (b) by concentrating the colloidal spheres in the vicinity of a solid surface, for example, in the vicinity of a substrate such as those described above, or in the vicinity of a temporary substrate, after which the microsphere assembly is transferred to a substrate such as those described above, for instance. Colloidal spheres may be concentrated, for example, (i) by sedimentation into a colloidal array in a gravitational field, (ii) by self-assembly under physical confinement, wherein the colloidal spheres are introduced as a colloidal dispersion into a space that confines the spheres while allowing removal of the liquid phase (e.g., using a packing assembly designed for this purpose or by using techniques as simple as a vacuum filtration), or (iii) by electrodeposition (e.g., charged microspheres, such as those described below, among others, may be electrodeposited onto a conductive substrate). Colloidal spheres may be concentrated, for example, either with or without sonication to assist with packing. For further information on these types of techniques, see, e.g., Y. Xia et al., supra, O. D. Velev et al., Nature, 2 Oct. 1997, Vol.389, 447-448, B. T. Holland et al., Science, 24 Jul. 1998, Vol. 281, 538-540 and J. Dutta et al., Encyclopedia of Nanoscience and Nanotechnology, Volume 9, pp. 617-640 (24).

With respect to electrodeposition, M. Yoldi et al., J. Mater. Sci., 41 (2006) 2965-2969, describe the electrophoretic deposition of colloidal crystals, both assisted by hydrodynamic flows and without. Briefly, aqueous colloidal dispersions of negatively charged monodisperse polystyrene latex spheres were deposited on a substrate corresponding to the anode of an electrochemical cell. Having filled the cell with the colloidal dispersion, voltage was applied by a DC power supply to generate an electric field sufficient to result in electrophoretic deposition, in which the negatively particles were observed to move to the positive electrode, accelerating the process of crystallization. Samples were prepared with a deposition duration of 4 h while applying voltages ranged from 2 V to 10 V. Samples were also prepared under constant applied voltage (5 V or 10 V) using varying deposition times. Once the colloidal crystal was formed, a higher voltage was applied during a shorter time (25 V, 5 min.) to make the spheres closer than their van der Waals distance, which joined them permanently. Colloidal crystals with a thickness from 1 to 40 layers were deposited. The number of layers was found to increase with the time of deposition. With regard to mechanism, they suggest an initial period in which the colloidal particles were quickly deposited on the substrate because they were repelled by the negative electrode and attracted by the positive one (i.e. the substrate), whereas at longer times, the influence of the positive electrode was reduced due to the screening effect of the deposited layers, at which times deposition is governed mainly by repulsive forces on the particles due to the negative electrode.

The cathode and anode (substrate) employed in Yoldi et al. are planar and parallel. For deposition on a non-planar substrate, however, other configurations may be preferable. For example, FIG. 2 is a schematic illustration of an electrochemical apparatus for electrophoretic deposition of colloidal crystals on the luminal surface of a stent 200 (end view) in accordance with an embodiment of the invention. A microsphere-containing colloidal suspension 220 is placed between the stent 200 and cylindrical counterelectrode 210 (end view). Multilayer colloidal crystals may be deposited from the suspension 220 onto the stent 200 upon application of an appropriate voltage (using a suitable voltage source 230) for an appropriate time. In this regard, the DC bias used for the electrodeposition of positively charged spheres will be opposite that employed for the electrodeposition of negatively charged spheres.

Three dimensional colloid sphere arrays may also be assembled using so-called layer-by-layer techniques in which in which charged substrates may be coated using various charged materials via electrostatic self-assembly. In a typical layer-by-layer process, multilayer growth proceeds through sequential steps, in which a substrate is alternately immersed in solutions of cationic and anionic materials, frequently with rinsing between steps. In this way, a first layer having a first surface charge is typically deposited (or adsorbed) on an underlying charged substrate, followed by a second layer having a second surface charge that is opposite the surface charge of the first layer, and so forth. The charge on the outer layer is reversed upon deposition of each sequential layer. Cationic and anionic materials include charged particles and polyelectrolytes (i.e., charged polymers and their precursors). Typically, 2 to 5 to 10 to 25 to 50 or more layers are applied using this technique.

Layer-by-layer techniques are attractive techniques for creating three-dimensional microsphere arrays on substrates having complex 3D contours (e.g., stents), because the various layers may be formed by simply dipping the substrates into suitable solutions in a suitable order.

As defined herein, the term “polyelectrolyte” embraces various charged polymers and their precursors including polycations and their precursors (e.g., polybases, polysalts, etc.), polyanions and their precursors (e.g., polyacids, polysalts, etc.), polymers having both anionic and cationic groups yet having an overall net positive or negative charge (e.g., polymers having multiple acidic and basic groups such as are found in various proteins), ionomers (polyelectrolytes in which a small but significant proportion of the constitutional units carry charges), and so forth.

Specific examples of suitable polycations may be selected, for instance, from the following: polyamines, including polyamidoamines, poly(amino methacrylates) including poly(dialkylaminoalkyl methacrylates) such as poly(dimethylaminoethyl methacrylate) and poly(diethylaminoethyl methacrylate), polyvinylamines, polyvinylpyridines including quaternary polyvinylpyridines such as poly(N-ethyl-4-vinylpyridine), poly(vinylbenzyltrimethylamines), polyallylamines such as poly(allylamine hydrochloride) (PAH) and poly(diallyldialklylamines) such as poly(diallyldimethylammonium chloride), spermine, spermidine, hexadimethrene bromide (polybrene), polyimines including polyalkyleneimines such as polyethyleneimine, polypropyleneimine and ethoxylated polyethyleneimine, basic peptides and proteins, including histone polypeptides and homopolymer and copolymers containing lysine, arginine, omithine and combinations thereof, gelatin, albumin, protamine and protamine sulfate, and polycationic polysaccharides such as cationic starch and chitosan, as well as copolymers, derivatives and combinations of the preceding, among various others.

Specific examples of suitable polyanions may be selected, for instance, from the following: polysulfonates such as polyvinylsulfonates, poly(styrenesulfonates) such as poly(styrene sulfonate) (PSS), sulfonated poly(tetrafluoroethylene), as well as sulfonated versions of various other homopolymers and copolymers, polysulfates such as polyvinylsulfates, sulfated and non-sulfated glycosaminoglycans as well as certain proteoglycans, for example, heparin, heparin sulfate, chondroitin sulfate, keratan sulfate, dermatan sulfate, polycarboxylates such as acrylic acid polymers and salts thereof (e.g., ammonium, potassium, sodium, etc.) (PAA), for instance, those available from Atofina and Polysciences Inc., methacrylic acid polymers and salts thereof (e.g., EUDRAGIT, a methacrylic acid and ethyl acrylate copolymer), carboxymethylcellulose, carboxymethylamylose and carboxylic acid derivatives of various other polymers, polyanionic peptides and proteins such as glutamic acid polymers and copolymers, aspartic acid polymers and copolymers, polymers and copolymers of uronic acids such as mannuronic acid, galatcuronic acid and guluronic acid, and their salts, for example, alginic acid and sodium alginate, hyaluronic acid, gelatin and carrageenan, polyphosphates such as phosphoric acid derivatives of various polymers, polyphosphonates such as polyvinylphosphonates, as well as copolymers, derivatives and combinations of the preceding, among various others.

In the present invention, two- and three-dimensional microsphere arrays may be assembled on a charged substrate using charged microspheres and, optionally, polyelectrolytes.

Substrate and microsphere materials may be inherently charged. For example, a polymeric material may be charged because the species used in its formation (e.g., initiators, monomers, etc.) are charged.

To the extent that the substrate and/or the microspheres are not inherently charged, they may be provided with a charge, for example, by exposing the substrate or microspheres to a suitable plasma, by adsorbing a suitable polyelectrolyte on the substrate or microspheres, and so forth. An amphiphilic polycation such as PEI is commonly used for the latter purpose, as it strongly promotes adhesion to a variety of substrates. This process has been demonstrated on glass substrates using charged polymeric (polyelectrolyte) materials. See, e.g., “Multilayer on solid planar substrates,” Multi-layer thin films, sequential assembly of nanocomposite materials, Wiley-VCH ISBN 3-527-30440-1, Chapter 14; and Hau, Winky L. W. et al. “Surface-chemistry technology for microfluidics,” J. Micromech. Microeng. 13 (2003) 272-278. PSS may be employed as a polyanion for this purpose, either applied directly, or after the formation of a positively charged layer (e.g., a PEI layer). In this regard, the surface charge of a given substrate or microsphere may readily be reversed by exposing it to a polyelectrolyte of opposite charge as described in the paragraph to follow. As another example, polyelectrolytes such as PAA and PSS may be grafted onto a substrate or microsphere by atom transfer radical polymerization (ATRP) from initiating sites previously anchored onto the substrate or microsphere using techniques analogous to those described in H. Kong et al., Polymer 46 (2005) 2472-2485.

To the extent that the microspheres (or the substrate) are inherently positively charged (or have been made positively charged), all or a portion of the microspheres (or the substrate) may be negatively charged by adsorbing a polyanion onto the microspheres (or the substrate) in an electrostatic self-assembly step. Conversely, to the extent that the microspheres (or the substrate) are inherently negatively charged (or have been made negatively charged), all or a portion of the microspheres (or the substrate) can be positively charged by adsorbing a polycation onto the microspheres (or the substrate) in a self-assembly step.

To build multilayer structures, in some embodiments, positively charged microspheres may be adsorbed upon a negatively charged substrate, followed by negatively charged microspheres, and so forth. Conversely, in some embodiments, negatively charged microspheres may be adsorbed on a positively charged substrate, followed by positively charged microspheres, and so forth.

In other embodiments, multilayer structures may be built by adsorbing a layer of positively charged microspheres on a negatively charged substrate, followed by a polyelectrolyte layer (i.e., a polyanionic layer), followed by a layer of positively charged microspheres, and so forth. Conversely, in some embodiments, a layer of negatively charged microspheres may be formed on a positively charged substrate, followed by a polyelectrolyte layer (i.e., a polycationic layer), followed by a layer of negatively charged microspheres, and so forth.

It should be clear from the foregoing that the number of possible combinations of microsphere and polyelectrolyte layers is near-infinite.

As indicated above, in an aspect of the invention, once a predecessor structure is formed that comprises an assembly of microspheres disposed on a substrate, then that assembly of microspheres may be used as a template for the formation of a porous layer.

For example, in some embodiments, the microspheres may contain one or more types of radiation degradable polymers, in which case the predecessor structure may be irradiated with electrons under conditions suitable to create a porous layer (e.g., in a vacuum, with electron beam irradiation having a fluence and current density suitable to render the assembly porous). The predecessor structure may be irradiated under either static (i.e., stationary) conditions or dynamic conditions (e.g., while being rotated, etc.). For example, rotation during irradiation is particularly desirable for cylindrical and tubular medical devices such as stents.

S. O. Cho et al., Adv. Mater., 6 Jan. 2005, 17(1), 120-125 describe suitable conditions for forming porous layers from microsphere assemblies. In Cho et al., colloidal crystals formed from monodisperse PMMA microspheres (having diameters of 150 nm, 400 nm, and 1500 nm) were irradiated with electrons to form macroporous PMMA layers. They found that the central part of each PMMA microsphere was removed more rapidly by the electron beam than the periphery. As a result, pore formation was initiated near the center of each microsphere. As the electron fluence increased, the polymeric materials were slowly removed outwards from the center of each microsphere, thereby gradually increasing the pore size. Microscopic images of two electron beam irradiated planar samples from Cho et al. are shown in FIGS. 1A and 1B (scale bar=400 nm). An irradiated non-planar sample is shown in FIG. 1C (scale bar=1000 nm). Such porous samples comprise interconnected hexagonally close packed spherical pores. Without wishing to be bound by theory, Cho et al. hypothesize that the observed pore forming phenomenon is related to an increased likelihood of electron track overlap at the center of a spherical particle coupled with the belief that the PMMA particles become charged under the electron irradiation. The electric field generated from the charged spherical-shaped PMMA particle is believed to act as a focusing lens for the electrons entering the particles, such that the electrons are deflected inwards to the particle center, causing the central part of the sphere to degrade faster an the periphery. In Cho et al., ambient pressure during irradiation was 2×10⁻⁵ torr, beam energy was varied from 40 to 60 keV, current density was varied from 0.1 to 20 μA cm⁻², and total electron fluence was varied from 2×10¹⁴ cm⁻² to 1×10¹⁸ cm⁻². Total electron fluence and electron beam current density were reported to be key parameters for the creation of porous materials, with a critical electron fluence above a certain level being required for pore creation. Critical electron fluence varied with current density. For example, for a monolayer of 400 nm PMMA spheres exposed to an electron beam having a current density of 5 μA cm⁻², a total electron fluence of 5×10¹⁵ cm⁻² was sufficient for pore formation. A lesser current density of 0.5 μA cm⁻², on the other hand, required a total electron fluence of 2×10¹⁶ cm⁻² for pore formation. The primary indicator of pore size was the size of the polymer microspheres themselves. Porosity was controlled by changing the electron beam fluence, with pore size increasing with electron fluence. Ultimately a point is reached where the porous structure is destroyed due to the removal of too much PMMA, although this is clearly an undesirable outcome for purposes of the present invention.

PMMA microspheres having diameters ranging from about 100 nm up to tens of microns are available commercially. Negatively charged PMMA microspheres are commercially available from Soken Chemicals Co., Tokyo, Japan (see F. Tang et al., J. Am. Ceram. Soc., 86 (12) 2050-54 (2003), which microspheres can be deposited on a conductive substrate by electrophoresis or on a charged substrate by electrostatic deposition, among other techniques. The size selected will depend upon the pore size that is desired.

In other embodiments, a precursor structure is formed that comprises a substrate, an assembly of radiation-degradable polymer microspheres (e.g., PMMA microspheres, etc.) disposed over the substrate, and a radiation-stable polymer layer disposed over the assembly of radiation-degradable polymer microspheres (e.g., by over-spraying). By irradiating such a precursor structure with radiation such as electron beam radiation, a microsphere assembly may be created which has an inner porous structure. Access to the inner porous structure may be obtained, for example, by puncturing and/or removing the outer radiation-stable polymer layer.

In other embodiments, interstices between the microspheres of a precursor structure (which comprises an assembly of microspheres disposed on a substrate) are infiltrated with a material in fluid form (e.g., in liquid form, in particulate form, in the form of particles suspended in a liquid, etc.), followed by solidification of the material, after which the microspheres are removed. Solidifiable fluid materials for this purpose include heat-fusible particles, polymerizable fluids (e.g., fluids polymerizable by exposure to ultraviolet radiation or heat), polymer melts (which solidify on cooling), polymer solutions (which solidify upon solvent removal), and sol-gel precursors (which solidify into ceramic materials due to hydrolysis and condensation reactions). Examples of the latter include alkoxides (e.g., a methoxide, ethoxide, isopropoxide, tert-butoxide, etc.) of various semi-metals and metals (e.g., silicon, germanium, aluminum, zirconium, titanium, tin, iron, hafnium, tantalum, molybdenum, tungsten, rhenium, iridium, etc.)

After solidification of the fluid material, the microspheres are removed, yielding a porous layer. For example, microspheres may be removed by thermal decomposition or by dissolution, among other processes. A wide range of organic (e.g., polymeric, etc.) and inorganic (e.g., ceramic, metallic, etc.) microspheres can be used for this purpose. For example, polystyrene microspheres may be employed and removed by thermal decomposition or by dissolution in an organic solvent such as toluene (e.g., where the interstices are filled with a sol-gel-derived ceramic material or a polymer that is insoluble in the organic solvent), or silica microspheres may be employed and removed by etching in hydrofluoric acid (e.g., where the interstices are filled with a hydrofluoric-acid-resistant polymer), among other possibilities. For further information concerning the formation of porous materials using colloidal crystals, see H.-P. Hentze et al., Reviews in Molecular Biotechnology 90 2002 27-53 and M. L. K. Hoa et al., Advances in Colloid and Interface Science 121 (2006) 9-23.

Using techniques such as those described above, a variety of porous layers can be formed including biostable porous polymeric layers, biodegradable polymeric layers, ceramic layers, and ceramic-polymeric hybrid layers. Suitable polymeric and ceramic materials can be selected from those set forth above for use as substrate materials.

In some embodiments, pore sizes of about 100 nm or less are preferred. In certain embodiments, for example, those where endothelial cell growth is desired, pore sizes are preferably less than 20 nm. In this regard, Dalby et al. investigated cell response to nanostructured surfaces created with polymer demixing, e.g. blends of hydrophobic polystyrene and hydrophilic poly(4-bromostyrene) undergo separation during spin casting. They created shallow islands of 13, 35 and 95 nm. Of the three nanotopographies, the 13 nm island gave the largest response to human endothelial cell, with highly spread morphologies containing well defined cytoskeleton. See M. J. Dalby et al., “In vitro reaction of endothelial cells to polymer demixed nanotopography,” Biomaterials 23 (2002) 2945-2954; see also Yap et al., supra. Moreover, in addition to surface feature size, surface chemistry also plays a role in cell adhesion and proliferation. See D. C. Miller et al., “Endothelial and vascular smooth muscle cell function on poly(lactic-co-glycolic acid) with nano-structured surface features,” Biomaterials 25 (2004) 53-61.

Porous layers in accordance with the present invention may be loaded with a suitable therapeutic agent using a variety of techniques.

In some embodiments, porous layers are formed from a material that further comprises one or more therapeutic agents. For example, a therapeutic agent may be included within the radiation-degradable microspheres or within the solidifiable fluid materials described above. As a specific example, a first therapeutic agent may be admixed with a polymer or conjugated to a polymer and provided within a biodegradable porous polymeric layer using such techniques. As another specific example, a first therapeutic agent may be provided at the surface of the radiation-degradable microspheres (e.g., conjugated to the microsphere surface, provided in a layer on the microsphere surface, etc.), and the central part of the microsphere ultimately preferably removed, for example, as described in S. O. Cho et al., supra. The first therapeutic agent in these examples may be released, for instance, by diffusion, polymer biodegradation, or both. If desired, a second therapeutic agent can be provided within the pores of the porous layer, for example, as described below. The second therapeutic agent in these examples may be released, for instance, by diffusion, polymer biodegradation, or both. In certain of these embodiments, the second therapeutic agent elutes first from the pores, whereas the second therapeutic agent is released as the polymer biodegrades.

In some embodiments, porous layers are formed first and subsequently loaded with one or more therapeutic agents.

For example, a solution or dispersion containing or more solvent species, one or more therapeutic agents and one or more optional species (e.g., one or more biodegradable polymers selected from one of those below, among others) may be applied to the porous layer and the solvent removed. As another example, a polymer melt containing one or more biodegradable polymers, one or more therapeutic agents and one or more optional species may be applied to the porous layer and cooled. Such solutions, dispersions or melts may be applied to porous layers using various suitable techniques, such as spin coating techniques, web coating techniques, spraying techniques, dipping techniques, ink jet printing techniques, electrostatic techniques, extrusion techniques, techniques involving coating via mechanical suspension including air suspension, and combinations of these techniques, among others.

As another example, in certain embodiments where a charged therapeutic agent is employed (e.g., by virtue of being inherently charged or by covalently or non-covalently associating the therapeutic agent with a charged species), one or more layers of the charged therapeutic agent may be deposited over the surface of the porous layer (including the interior surfaces associated with the pores) using layer-by-layer electrostatic self-assembly techniques such as those described above. For example, in some instances, the therapeutic agent is itself a polyelectrolyte (e.g., where the therapeutic agent is a polypeptide or a polynucleotide) and it is used as such to create one or more of the polyelectrolyte layers within a multilayer coating. In other instances, the charged therapeutic agent is not a polyelectrolyte (e.g., it may be a charged small molecule drug). Nevertheless, one or more layers of the charged therapeutic agent may be substituted for one or more polyelectrolyte layers during the multilayer assembly process. In each of these cases, the therapeutic agent is disposed within the polyelectrolyte multilayer coating. To the extent that the porous layer does not have a surface charge after electron beam irradiation that is sufficient for layer-by-layer assembly, it may be treated to provide it with a surface charge as described above (e.g., by plasma treatment or dipping in a solution of polyethyleneimine), among other treatments.

In some embodiments, the porous layer surface (including the interior surface associated with the pores) is lined with a material that renders the porous layer more hydrophobic or more hydrophilic, prior to therapeutic agent loading.

For example, where charged, the porous layer surface may be treated with a lipid having an opposite charge. This will render the surface more hydrophobic, allowing for the application of a hydrophobic therapeutic agent or a hydrophobic therapeutic agent dispersed in a hydrophobic carrier (e.g., an oil based cancer formulation such as paclitaxel, which is commonly dissolved in a hydrophobic solvent such as soybean oil, castor oil, squalane or dodecane).

As a specific example, in N. Kohli et al., “Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates,” Journal of Colloid and Interface Science 301 (2006) 461-469, liposomes composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoylsn-glycero-3-phosphate (monosodium salt) (DOPA) were adsorbed to poly(dimethyldiallylammonium chloride) (PDAC) and poly(allylamine hydrochloride) (PAH) surfaces. Lipid bilayers composed of negatively charged lipids like (DOPA, 1-stearoyl-2-oleoyl-phosphatidylserine (SOPS), and 1,2-dimyristoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (DMPG) blended with other zwitterionic lipids such as DOPC and 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) have also been shown to form on polyelectrolyte surfaces such as PAH, PEI, and PDAC coated substrates. Id.

Thus, in one embodiment of the invention, once a given porous layer is provided with a surface charge (e.g., by providing a polyelectrolyte coating on the porous structure), one can then cover the charged porous layer with a hydrophobic lipid layer, after which this structure may be loaded with, for example, a soybean oil containing paclitaxel.

As another example, the porous layer may be rendered super-hydrophilic (i.e., defined herein as having contact angles of less than 10°), for example, by the deposition of titanium oxide nanoparticles in accordance with D. S. Kommireddy et al., Journal of Nanoscience and Nanotechnology, 2005, Vol.5, 1081-1087, in which stable, super-hydrophilic (water contact angle ≈0°) films were formed on PMMA, among other materials, using a layer-by-layer method wherein alternating deposition of titanium dioxide nanoparticles and poly(styrene sulfonate) was used to form films of thickness ranging from 11 nm to 220 nm. The hydrophilicity of these thin films increased with increasing number of deposited PSS/TiO₂ bilayers. Such a porous hydrophilic surface would have a sponge-like effect on hydrophilic liquids, including aqueous therapeutic-agent-containing solutions.

Once loaded with therapeutic agent, in certain embodiments of the invention, the porous layer may be over-coated with one or more additional layers that delay the release of the therapeutic agent, that render the porous layer lubricious, and so forth.

For example, a biodegradable layer may be applied over the therapeutic-agent-loaded porous layer. For instance, a solution or melt of a biodegradable polymer or polymer blend may be applied over a therapeutic-agent-loaded porous layer using a suitable technique, such as one of those described above (e.g., spray coating, etc.) Suitable biodegradable polymers include the following, among many others: (a) polyester homopolymers and copolymers such as those formed from one or more of the following: glycolide, D-lactide, L-lactide, beta-hydroxybutyrate, D-gluconate, L-gluconate, epsilon-caprolactone, delta-valerolactone, p-dioxanone, and trimethylene carbonate among others, for example, polyglycolide, poly (L-lactide) poly(D,L-lactide), poly(L-lactide-co-glycolide), poly(D,L-lactide-co-glycolide), and so forth, (b) poly(ortho esters) such as those synthesized by copolymerization of various diketene acetals and diols, among others, (c) polyanhydrides, and (d) amino-acid-based polymers including tyrosine-based polymers, among others.

As another example, release may be delayed by applying a layer of sputtered material, for example, a porous layer of a biostable metal such as tantalum or gold or a porous or non-porous layer of a biodegradable metal such as iron, magnesium or zinc, over the therapeutic-agent-loaded porous layer. As a specific example, a system for performing such a deposition is available from Mantis Deposition Ltd., Thame, Oxfordshire, United Kingdom, who market a high-pressure sputtering source which is able to generate particles from a sputter target with as few as 30 atoms up to those with diameters exceeding 15 nm. Systems like the Mantis Deposition Ltd. system can produce particle streams, the majority of which (approximately 80%) have a charge of one electron. Consequently, a magnetic field or a secondary electric field can be used to separate particles of similar weight from one another (because lighter particles are deflected to a greater degree in a given field than are the larger particles of the same charge). For example, the above Mantis Deposition Ltd. system is able to produce charged particle streams with a very narrow mass distribution. A system similar to the Mantis system can be obtained from Oxford Applied Research, Witney, Oxon, UK. Such processes are room temperature processes. Using these and similar systems, thin metallic layers may be deposited on a variety of substrates.

As yet another example, in certain embodiments, a polyelectrolyte multilayer coating may be applied over a therapeutic-agent-loaded porous layer. For instance, the pores may be filled with a therapeutic agent of relatively low water solubility (e.g., using a solution, dispersion or melt-based method such as that described above), allowing the polyelectrolyte multilayer coating to be applied from aqueous solutions with relatively low loss of therapeutic agent. In other embodiments, the porous layer is loaded with therapeutic agent subsequent to the application of a polyelectrolyte multilayer coating. For example, the pores may be filled with a removable material, followed by: (a) deposition of a polyelectrolyte multilayer coating, (b) removal of the removable material and (c) introduction of a therapeutic agent. As another example, where the pore sizes are sufficiently small such that they are closed by the deposition of the polyelectrolyte layers, enclosed pockets can be created. The therapeutic agent can then be introduced into these pockets. For further information, see, e.g., Pub. No. US 2005/0208100 A1 to Weber et al.

“Therapeutic agents,” “drugs,” “pharmaceutically active agents,” “pharmaceutically active materials,” and other related terms may be used interchangeably herein. These terms include genetic therapeutic agents, non-genetic therapeutic agents, and cells. Therapeutic agents may be used singly or in combination.

Exemplary non-genetic therapeutic agents for use in conjunction with the present invention include: (a) anti-thrombotic agents such as heparin, heparin derivatives, urokinase, and PPack (dextrophenylalanine proline arginine chloromethylketone); (b) anti-inflammatory agents such as dexamethasone, prednisolone, corticosterone, budesonide, estrogen, sulfasalazine and mesalamine; (c) antineoplastic/antiproliferative/anti-miotic agents such as paclitaxel, 5-fluorouracil, cisplatin, vinblastine, vincristine, epothilones, endostatin, angiostatin, angiopeptin, monoclonal antibodies capable of blocking smooth muscle cell proliferation, and thymidine kinase inhibitors; (d) anesthetic agents such as lidocaine, bupivacaine and ropivacaine; (e) anti-coagulants such as D-Phe-Pro-Arg chloromethyl ketone, an RGD peptide-containing compound, heparin, hirudin, antithrombin compounds, platelet receptor antagonists, anti-thrombin antibodies, anti-platelet receptor antibodies, aspirin, prostaglandin inhibitors, platelet inhibitors and tick antiplatelet peptides; (f) vascular cell growth promoters such as growth factors, transcriptional activators, and translational promotors; (g) vascular cell growth inhibitors such as growth factor inhibitors, growth factor receptor antagonists, transcriptional repressors, translational repressors, replication inhibitors, inhibitory antibodies, antibodies directed against growth factors, bifunctional molecules consisting of a growth factor and a cytotoxin, bifunctional molecules consisting of an antibody and a cytotoxin; (h) protein kinase and tyrosine kinase inhibitors (e.g., tyrphostins, genistein, quinoxalines); (i) prostacyclin analogs; (j) cholesterol-lowering agents; (k) angiopoietins; (l) antimicrobial agents such as triclosan, cephalosporins, aminoglycosides and nitrofurantoin; (m) cytotoxic agents, cytostatic agents and cell proliferation affectors; (n) vasodilating agents; (o) agents that interfere with endogenous vasoactive mechanisms; (p) inhibitors of leukocyte recruitment, such as monoclonal antibodies; (q) cytokines; (r) hormones; (s) inhibitors of HSP 90 protein (i.e., Heat Shock Protein, which is a molecular chaperone or housekeeping protein and is needed for the stability and function of other client proteins/signal transduction proteins responsible for growth and survival of cells) including geldanamycin, (t) alpha receptor antagonist (such as doxazosin, Tamsulosin) and beta receptor agonists (such as dobutamine, salmeterol), beta receptor antagonist (such as atenolol, metaprolol, butoxamine), angiotensin-II receptor antagonists (such as losartan, valsartan, irbesartan, candesartan and telmisartan), and antispasmodic drugs (such as oxybutynin chloride, flavoxate, tolterodine, hyoscyamine sulfate, diclomine), (u) bARKct inhibitors, (v) phospholamban inhibitors, (w) Serca 2 gene/protein, (x) immune response modifiers including aminoquizolines, for instance, imidazoquinolines such as resiquimod and imiquimod, and (y) human apolioproteins (e.g., AI, AII, AIII, AIV, AV, etc.).

Specific examples of non-genetic therapeutic agents include paclitaxel (including polymer-drug conjugates such as paclitaxel-polyglutamic acid conjugates and including particulate forms thereof, for instance, protein-bound paclitaxel particles such as albumin-bound paclitaxel nanoparticles, e.g., ABRAXANE), rapamycin and analogs thereof (e.g., sirolimus, everolimus, tacrolimus, zotarolimus, biolimus, pimecrolimus, and polymer-drug conjugates thereof such as everolimus-polyglutamic acid conjugates), Epo D, dexamethasone, estradiol, halofuginone, cilostazole, geldanamycin, ABT-578 (Abbott Laboratories), trapidil, liprostin, Actinomcin D, Resten-NG, Ap-17, abciximab, clopidogrel, Ridogrel, beta-blockers, bARKct inhibitors, phospholamban inhibitors, Serca 2 gene/protein, imiquimod, human apolioproteins (e.g., AI-AV), growth factors (e.g., VEGF-2), as well a derivatives of the forgoing, among others.

Exemplary genetic therapeutic agents for use in conjunction with the present invention include anti-sense DNA and RNA as well as DNA coding for the various proteins (as well as the proteins themselves): (a) anti-sense RNA, (b) tRNA or rRNA to replace defective or deficient endogenous molecules, (c) angiogenic and other factors including growth factors such as acidic and basic fibroblast growth factors, vascular endothelial growth factor, endothelial mitogenic growth factors, epidermal growth factor, transforming growth factor α and β, platelet-derived endothelial growth factor, platelet-derived growth factor, tumor necrosis factor α, hepatocyte growth factor and insulin-like growth factor, (d) cell cycle inhibitors including CD inhibitors, and (e) thymidine kinase (“TK”) and other agents useful for interfering with cell proliferation. Also of interest is DNA encoding for the family of bone morphogenic proteins (“BMP's”), including BMP-2, BMP-3, BMP-4, BMP-5, BMP-6 (Vgr-1), BMP-7 (OP-1), BMP-8, BMP-9, BMP-10, BMP-11, BMP-12, BMP-13, BMP-14, BMP-15, and BMP-16. Currently preferred BMP's are any of BMP-2, BMP-3, BMP-4, BMP-5, BMP-6 and BMP-7. These dimeric proteins can be provided as homodimers, heterodimers, or combinations thereof, alone or together with other molecules. Alternatively, or in addition, molecules capable of inducing an upstream or downstream effect of a BMP can be provided. Such molecules include any of the “hedgehog” proteins, or the DNA's encoding them.

Vectors for delivery of genetic therapeutic agents include viral vectors such as adenoviruses, gutted adenoviruses, adeno-associated virus, retroviruses, alpha virus (Semliki Forest, Sindbis, etc.), lentiviruses, herpes simplex virus, replication competent viruses (e.g., ONYX-015) and hybrid vectors; and non-viral vectors such as artificial chromosomes and mini-chromosomes, plasmid DNA vectors (e.g., pCOR), cationic polymers (e.g., polyethyleneimine, polyethyleneimine (PEI)), graft copolymers (e.g., polyether-PEI and polyethylene oxide-PEI), neutral polymers PVP, SP1017 (SUPRATEK), lipids such as cationic lipids, liposomes, lipoplexes, nanoparticles, or microparticles, with and without targeting sequences such as the protein transduction domain (PTD).

Cells for use in conjunction with the present invention include cells of human origin (autologous or allogeneic), including whole bone marrow, bone marrow derived mono-nuclear cells, progenitor cells (e.g., endothelial progenitor cells), stem cells (e.g., mesenchymal, hematopoietic, neuronal), pluripotent stem cells, fibroblasts, myoblasts, satellite cells, pericytes, cardiomyocytes, skeletal myocytes or macrophage, or from an animal, bacterial or fungal source (xenogeneic), which can be genetically engineered, if desired, to deliver proteins of interest.

Numerous therapeutic agents, not necessarily exclusive of those listed above, have been identified as candidates for vascular treatment regimens, for example, as agents targeting restenosis. Such agents are useful for the practice of the present invention and include one or more of the following: (a) Ca-channel blockers including benzothiazapines such as diltiazem and clentiazem, dihydropyridines such as nifedipine, amlodipine and nicardapine, and phenylalkylamines such as verapamil, (b) serotonin pathway modulators including: 5-HT antagonists such as ketanserin and naftidrofuryl, as well as 5-HT uptake inhibitors such as fluoxetine, (c) cyclic nucleotide pathway agents including phosphodiesterase inhibitors such as cilostazole and dipyridamole, adenylate/Guanylate cyclase stimulants such as forskolin, as well as adenosine analogs, (d) catecholamine modulators including α-antagonists such as prazosin and bunazosine, β-antagonists such as propranolol and α/β-antagonists such as labetalol and carvedilol, (e) endothelin receptor antagonists, (f) nitric oxide donors/releasing molecules including organic nitrates/nitrites such as nitroglycerin, isosorbide dinitrate and amyl nitrite, inorganic nitroso compounds such as sodium nitroprusside, sydnonimines such as molsidomine and linsidomine, nonoates such as diazenium diolates and NO adducts of alkanediamines, S-nitroso compounds including low molecular weight compounds (e.g., S-nitroso derivatives of captopril, glutathione and N-acetyl penicillamine) and high molecular weight compounds (e.g., S-nitroso derivatives of proteins, peptides, oligosaccharides, polysaccharides, synthetic polymers/oligomers and natural polymers/oligomers), as well as C-nitroso-compounds, β-nitroso-compounds, N-nitroso-compounds and L-arginine, (g) Angiotensin Converting Enzyme (ACE) inhibitors such as cilazapril, fosinopril and enalapril, (h) ATII-receptor antagonists such as saralasin and losartin, (i) platelet adhesion inhibitors such as albumin and polyethylene oxide, (j) platelet aggregation inhibitors including cilostazole, aspirin and thienopyridine (ticlopidine, clopidogrel) and GP IIb/IIa inhibitors such as abciximab, epitifibatide and tirofiban, (k) coagulation pathway modulators including heparinoids such as heparin, low molecular weight heparin, dextran sulfate and β-cyclodextrin tetradecasulfate, thrombin inhibitors such as hirudin, hirulog, PPACK(D-phe-L-propyl-L-arg-chloromethylketone) and argatroban, FXa inhibitors such as antistatin and TAP (tick anticoagulant peptide), Vitamin K inhibitors such as warfarin, as well as activated protein C, (l) cyclooxygenase pathway inhibitors such as aspirin, ibuprofen, flurbiprofen, indomethacin and sulfinpyrazone, (m) natural and synthetic corticosteroids such as dexamethasone, prednisolone, methprednisolone and hydrocortisone, (n) lipoxygenase pathway inhibitors such as nordihydroguairetic acid and caffeic acid, (o) leukotriene receptor antagonists, (p) antagonists of E- and P-selectins, (q) inhibitors of VCAM-1 and ICAM-1 interactions, (r) prostaglandins and analogs thereof including prostaglandins such as PGE1 and PG12 and prostacyclin analogs such as ciprostene, epoprostenol, carbacyclin, iloprost and beraprost, (s) macrophage activation preventers including bisphosphonates, (t) HMG-CoA reductase inhibitors such as lovastatin, pravastatin, fluvastatin, simvastatin and cerivastatin, (u) fish oils and omega-3-fatty acids, (v) free-radical scavengers/antioxidants such as probucol, vitamins C and E, ebselen, trans-retinoic acid and SOD mimics, (w) agents affecting various growth factors including FGF pathway agents such as bFGF antibodies and chimeric fusion proteins, PDGF receptor antagonists such as trapidil, IGF pathway agents including somatostatin analogs such as angiopeptin and ocreotide, TGF-β pathway agents such as polyanionic agents (heparin, fucoidin), decorin, and TGF-β antibodies, EGF pathway agents such as EGF antibodies, receptor antagonists and chimeric fusion proteins, TNF-α pathway agents such as thalidomide and analogs thereof, Thromboxane A2 (TXA2) pathway modulators such as sulotroban, vapiprost, dazoxiben and ridogrel, as well as protein tyrosine kinase inhibitors such as tyrphostin, genistein and quinoxaline derivatives, (x) MMP pathway inhibitors such as marimastat, ilomastat and metastat, (y) cell motility inhibitors such as cytochalasin B, (z) antiproliferative/antineoplastic agents including antimetabolites such as purine analogs (e.g., 6-mercaptopurine or cladribine, which is a chlorinated purine nucleoside analog), pyrimidine analogs (e.g.,. cytarabine and 5-fluorouracil) and methotrexate, nitrogen mustards, alkyl sulfonates, ethylenimines, antibiotics (e.g., daunorubicin, doxorubicin), nitrosoureas, cisplatin, agents affecting microtubule dynamics (e.g., vinblastine, vincristine, colchicine, Epo D, paclitaxel and epothilone), caspase activators, proteasome inhibitors, angiogenesis inhibitors (e.g., endostatin, angiostatin and squalamine), rapamycin (sirolimus) and its analogs (e.g., everolimus, tacrolimus, zotarolimus, etc.), cerivastatin, flavopiridol and suramin, (aa) matrix deposition/organization pathway inhibitors such as halofuginone or other quinazolinone derivatives and tranilast, (bb) endothelialization facilitators such as VEGF and RGD peptide, and (cc) blood rheology modulators such as pentoxifylline.

Further additional therapeutic agents useful for the practice of the present invention are also disclosed in U.S. Pat. No. 5,733,925 to Kunz.

EXAMPLE 1

Stainless steel stents, 24 mm, 4 mm diameter, Liberté™, available from Boston Scientific, are cleaned with an RF oxygen plasma for 5 minutes to remove surface contaminants. Polyethyleneimine (PEI), poly(allylamine hydrochloride) (PAH), and poly(sodium-4-styrene sulfonate) (PSS) are available from Aldrich. Each of these polyelectrolytes is provided in solutions having a concentration of 1 mM polyelectrolyte and 0.8 M NaCl. Negatively charged sulfate-stabilized polystyrene (PS) spheres of diameter 330 nm are available from MicroPartikel, Berlin, Germany. The PS particle suspension is 0.1% m/m in pure water. Using the different solutions, an initial four layers of PEI-PAH-PSS-PAH are coated on the stent surface by dipping for 10 minutes in the separate solutions and rinsing twice in pure water for 10 minutes. The fifth layer is made by dipping the stent into the solution having the PS spheres. After this, the stent is rinsed with water and dipped again in the PAH solution. This sequence is repeated five times to build a multi-layer structure of PS spheres surrounded by PAH. Following this step, the stent is rinsed twice in isopropyl alcohol and dried.

EXAMPLE 2

Stainless steel stents, 24 mm, 4 mm diameter, Liberté™, available from Boston Scientific, are cleaned with an RF oxygen plasma for 5 minutes to remove surface contaminants. Negatively charged sulfate-stabilized PS spheres of diameter 330 nm are available from MicroPartikel, Berlin, Germany. (Alternatively, negatively charged PS spheres of 975±10 nm diameter may be provided by Ikerlat Polymers as described in M. Yoldi et al., J. Mater. Sci., 41 (2006) 2965-2969.) Following procedures along the lines described in M. Yoldi et al., and using an electrochemical cell like that illustrated in FIG. 2, an aqueous colloidal dispersion of negatively charged polystyrene spheres in an aqueous-ethanolic medium is deposited on the interior surface of the stent, which corresponds to the positively charged electrode of the electrochemical cell, by applying a suitable DC voltage for a time sufficient to create a multilayer colloidal crystal (e.g., one having approximately 20 layers). Once the colloidal crystal is formed, a higher voltage is applied for a shorter time to join the spheres, and the stent is dried.

EXAMPLE 3

Stents in accordance with Example 1 and Example 2 are subjected to sol-gel processing by immersing the stents for 12 hours in a solution of 2 wt % TEOS (tetra-ethoxy-silane), 88 wt % ethanol, 9 wt % water, and 1 wt % ammonium hydroxide. The stents are then calcinated at 540° C. for 8 hours to remove the organic components and cooled overnight in the oven to room temperature, yielding stents with a multi-layer structure of hollow silica spheres.

EXAMPLE 4

Stents in accordance with Example 1 and Example 2 are subjected to sol-gel processing by immersing the stents in a TiO₂ sol synthesized by acid hydrolysis of titanium isopropoxide along the lines described in F. Sun et al., Chem. Mater. 2006, 18, 3774-3779 and Q. Y. Xu et al. J. Mater. Res. 6 (1991) 1073-1079. The stents are then calcinated at elevated temperature to remove the organic components and cooled overnight in the oven to room temperature, yielding stents with a multi-layer structure of hollow silica spheres.

EXAMPLE 5

Procedure analogous to that of Example 1 and Example 2 are employed, using negatively charged PMMA spheres, rather than negatively charged polystyrene spheres, to form multilayer structures of PMMA spheres. Negatively charged PMMA spheres may be obtained from sources such as those described in S. O. Cho et al., Adv. Mater., 6 Jan. 2005, 17(1), 120-125, among others.

EXAMPLE 6

The structures Example 5 are irradiated with electron beam radiation having a beam energy, current density and total electron fluence suitable to create porous PMMA layers, for example, in accordance with the procedures described in S. O. Cho et al., Adv. Mater., 6 Jan. 2005, 17(1), 120-125.

EXAMPLE 7

Polypyrrole (PPy) is coated on the structures of Example 5 by in-situ polymerization along the lines described in H. Dong et al., Langmuir, 22 (2006) 11384-11387. Briefly, the structures are immersed in an aqueous solution of pyrrole containing p-toluene sulfonic acid as a dopant. The polymerization of pyrrole (and the deposition of PPy on the PMMA spheres) is initiated at room temperature by the addition an aqueous ammonium persulfate solution. The coated spheres are rinsed with distilled water and dried in air.

EXAMPLE 8

Hollow PPy spheres are prepared by solvent extraction along the lines described in H. Dong et al., supra. In brief, the structures of Example 7 are immersed in chloroform and left to stand at room temperature overnight. The structure is removed from solution, washed several times with chloroform to enhance core removal, and air-dried, to yield stents with a multi-layer structure of hollow PPy spheres

EXAMPLE 9

Hollow carbon spheres are formed by thermolysis along the lines described in H. Dong et al., supra. Briefly, the dried structures of Example 7 are placed in a tube furnace for thermolysis. Under a N₂ atmosphere, the furnace temperature is raised to 1000° C. at a constant rate and held at 1000° C. for 1 h before cooling down to room temperature, to yield stents with a multi-layer structure of hollow carbon spheres.

EXAMPLE 10

Hollow PPy spheres are prepared. In brief, the structures of Example 7 (PMMA spheres coated with PPy) are irradiated, along the lines described in S. O. Cho et al., supra, with electron beam radiation having a beam energy, current density and total electron fluence suitable to remove a least a portion of the PMMA material at the center of the spheres. The resulting spherical pores may or may not be interconnected, depending on the processing parameters.

EXAMPLE 11

Polypyrrole (PPy) is coated on the structures of Example 5 by in-situ polymerization using a method analogous to that described in H. Dong et al., Langmuir, 22 (2006) 11384-11387. Briefly, the structures are immersed in an aqueous solution of pyrrole containing paclitaxel-polyglutamic acid conjugate as a dopant (rather than p-toluene sulfonic acid as in Dong et al.). The polymerization of pyrrole (and the deposition of PPy on the PMMA spheres) is initiated at room temperature by the addition an aqueous ammonium persulfate solution. The coated spheres are rinsed with distilled water and dried in air.

EXAMPLE 12

Hollow paclitaxel-containing PPy spheres are prepared. Briefly, the structures of Example 11 (PMMA spheres coated with paclitaxel-doped PPy) are irradiated, along the lines described in S. O. Cho et al., supra, with electron beam radiation having a beam energy, current density and total electron fluence suitable to remove a least a portion of the PMMA material at the center of the spheres. The resulting spherical pores may or may not be interconnected, depending on the processing parameters.

Although various embodiments are specifically illustrated and described herein, it will be appreciated that modifications and variations of the present invention are covered by the above teachings and are within the purview of the appended claims without departing from the spirit and intended scope of the invention.

Claims

1. A method of forming an implantable or insertable medical device comprising: (a) forming a predecessor structure that comprises an assembly of microspheres disposed over an underlying substrate, the microspheres carrying a first therapeutic agent, the first therapeutic agent being loaded to the microspheres by conjugating the first therapeutic agent with a surface of the microspheres or by forming a coating layer containing the first therapeutic agent on the microspheres,(b) mixing with or conjugating to a biodegradable polymer a second therapeutic agent and using the mixed or conjugated biodegradable polymer and the second therapeutic agent to form a biodegradable, porous layer over said substrate using said microspheres as templates for pores of the porous layer,(c) removing portions of or all of the microspheres to form the porous layer, with the pores containing the first therapeutic agent, and(d) loading the pores of the porous layer with a third therapeutic agent.

2. The method of claim 1, wherein said microspheres comprise a radiation degradable polymer and wherein said predecessor structure is irradiated with electrons such that said porous layer is created from degradation of said assembly of microspheres.

3. The method of claim 2, wherein said radiation degradable polymer is a homopolymer or copolymer comprising a monomer selected from methyl methacrylate, 2,2,2-trifluoroethyl-chloroacrylate, butene-1-sulfone, and combinations thereof.

4. The method of claim 2, wherein said medical device is rotated during electron irradiation.

5. The method of claim 1, wherein said porous layer is created by a method comprising (a) infiltrating interstices between the microspheres with a fluid of the mixed or conjugated biodegradable polymer and the first therapeutic agent, (b) solidifying said fluid, and (c) removing said microspheres to create said porous layer.

6. The method of claim 5, wherein said microspheres are selected from polymeric, ceramic and metallic microspheres.

7. The method of claim 5, wherein said fluid comprises heat-fusible particles, a polymerizable fluid, a polymer melt, a polymer solution, or a solution comprising a sol-gel precursor.

8. The method of claim 1, wherein said microspheres are less than 1 μm in diameter.

9. The method of claim 1, wherein said microspheres are less than 100 nm in diameter.

10. The method of claim 1, wherein the assembly is one microsphere in thickness.

11. The method of claim 1, wherein the assembly is ten or more microspheres in thickness.

12. The method of claim 1, wherein said porous layer is made more hydrophobic before loading said porous layer with said third therapeutic agent.

13. The method of claim 1, wherein said porous layer is made more hydrophilic before loading said porous layer with said third therapeutic agent.

14. The method of claim 1, further comprising applying a coating layer over the therapeutic-agent-loaded porous layer.

15. The method of claim 14, wherein said coating comprises a material selected from a polymer, a metal, a metal oxide and a combination of the same.

16. The method of claim 1, wherein said microspheres are monodisperse or polydisperse.

17. The method of claim 1, wherein the microspheres comprise a biodegradable polymer.

18. The method of claim 2, wherein creating the porous layer comprises removing the microspheres from a center of each microsphere outwardly.

19. The method of claim 5 or 18, wherein removing the microspheres comprises partially removing the microspheres.