Claims
- 1. A method of evaluating a solid phase for use in a dual bead assay, the method comprising selecting a test solid phase, binding a probe to the test solid phase in the presence or absence of a cross-linking agent, determining the total amount of probe bound to the test solid phase in the presence or absence of a cross-linking agent, determining the percentage of probe bound covalently to the solid phase, determining the amount of probe bound to the solid phase non-covalently, and calculating the percentage of probe bound covalently to the solid phase, wherein if no less than approximately 80% of the probe is bound covalently, the solid phase is suitable for use in a dual bead assay.
- 2. The method of claim 1, wherein the solid phase is a bead.
- 3. The method of claim 2, wherein the bead is a magnetic bead.
- 4. The method of claim 1, wherein the solid phase is a surface on a biodisc.
- 5. The method of claim 1, wherein the probe is a nucleic acid.
- 6. The method of claim 5, wherein the nucleic acid is double-stranded.
- 7. The method of claim 1, wherein the probe is a protein.
- 8. The method of claim 5 or 7, wherein the probe further comprises a linker.
- 9. The method of claim 8, wherein the linker is at least one polyethylene glycol moiety.
- 10. The method of claim 1, wherein the test solid phase is attached to a biodisc.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority from U.S. Provisional Application Serial No. 60/259,806, filed Jan. 4, 2001 and U.S. Provisional Application Serial No. 60/271,922, filed Feb. 27, 2001. These applications are hereby incorporated by reference into the subject application in their entireties.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60259806 |
Jan 2001 |
US |
|
60271922 |
Feb 2001 |
US |