Claims
- 1. A dyestuff composition for histological examination, which comprises
- (1) a product formed by the interaction of one molar proportion of thionin dyestuff selected from thionin, toluidine blue and dimethyl thionin with a molar proportion of resorcinol from about 0.2 to 5 moles at a temperature of from about 70 degrees centigrade to about 120 degrees centigrade,
- (2) 0.5(M.sub.T /M.sub.A) to 2(M.sub.T /M.sub.A) moles of a dyestuff "A", and
- (3) 0.5(M.sub.T /M.sub.B) to 2(M.sub.T /M.sub.B) moles of a dyestuff "B", wherein
- dyestuff "A" and dyestuff "B" are alkaline dyestuffs different in color and pH, both dyestuff "A" and dyestuff "B" can be mixtures of alkaline dyestuffs similar in color and pH,
- M.sub.T is the molecular weight of the thionin dyestuff,
- M.sub.A is the molecular weight of dyestuff "A", and
- M.sub.B is the molecular weight of dyestuff "B", or
- when any of the thionin dyestuff, dyestuff "A" and dyestuff "B" is a mixture of more substances, M.sub.T, M.sub.A and M.sub.B represent the weighed average of the molecular weights of the components of the thionin dyestuff, dyestuff "A" and dyestuff "B", respectively.
- 2. A dyestuff composition as claimed in claim 1, which comprises as component (1) a product formed by the interaction of one mole of a thionin dyestuff with 1 to 5 moles of resorcinol at elevated temperature.
- 3. A dyestuff composition as claimed in claim 1, which comprises as component (1) a product formed by the interaction of one mole of a histological dyestuff with 3 to 4 moles of resorcinol at elevated temperature.
- 4. A dyestuff composition as claimed in claim 1, which comprises as dyestuff "A" Alcyan blue 8 GX and as dyestuff "B" safranin.
- 5. A dyestuff composition as claimed in claim 1, which comprises dyestuffs "A" and "B" in a molar ratio of 0.5(M.sub.A /M.sub.B) to 2(MA/M.sub.B), wherein M.sub.A and M.sub.B are as defined in claim 1.
- 6. A dyestuff composition which comprises the reaction product obtained by the reaction of 1 mole equivalent of thionin dyestuff and 2 to 5 moles of resorcinol carried out at a temperature of 70 to 120 degrees centigrade, 0.2 to 0.4 mole equivalents of alcyan blue and 0.2 to 0.4 mole equivalents of safranin.
- 7. The dyestuff composition as claimed in claim 6, which comprises 0.3 moles of safranin and 0.3 moles of Alcyan blue calculated for a product formed from the interaction of 1 mole of thionin with 4 moles of resorcinol.
- 8. The dyestuff composition according to claim 7 wherein the thionin dyestuff comprises thionin.
- 9. The dyestuff composition according to claim 7 wherein the thionin dyestuff comprises toloudine blue.
- 10. The dyestuff composition according to claim 7 wherein the thionin dyestuff comprises dimethylthionin.
- 11. A dyestuff composition for histological examinations comprising a product formed by the interactions of the following components:
- (1) one mole part of a member of the group of thionin dyestuffs consisting of thionin, toluidine blue, dimethyl thionin, and mixtures thereof,
- (2) from about 0.2 to 5 mole parts of resorcinol,
- (3) 0.5(M.sub.T /M.sub.A) to 2(M.sub.T /M.sub.A) mole parts of a first alkaline dyestuff,
- (4) 0.5(M.sub.T /M.sub.B) to 2(M.sub.T /M.sub.B) mole parts of a second alkaline dyestuff differing from the first alkaline dyestuff with regard to color and pH;
- wherein the interaction of the components (1) and (2) is performed at a temperature of from about 70 degrees centigrade to about 120 degrees centigrade and wherein the components (3) and (4) are added successively to the interacted composition of components (1) and (2).
- 12. The dyestuff composition according to claim 10 wherein the interactiom composition of components (1) and (2) is cooled down before the addition of components (3) and (4).
- 13. The dyestuff composition according to claim 10 wherein the first alkaline dyestuff is a mixture of alkaline dyestuffs similar in color and pH.
- 14. The dystuff composition according to claim 10 wherein the second alkaline dystuff is a mixture of alkaline dyestuffs similar in color and pH.
- 15. The dystuff composition according to claim 10 wherein the amount of resorcinol employed is from about 1 to 5 moles.
- 16. The dystuff composition according to claim 10 wherein the amount of resorcinol employed is from about 3 to 4 moles.
- 17. The dystuff composition according to claim 10 wherein the amount of first alkaline dyestuff employed is from about 0.5 to 2 moles.
- 18. The dystuff composition according to claim 10 wherein the amount of second alkaline dyestuff employed is from about 0.5 to 2 moles.
- 19. The dystuff compositionaccording to claim 10 wherein the amount of second alkaline dyestuff employed is from about 0.5 to 2 times the number of moles employed of the first alkaline dyestuff.
Priority Claims (1)
Number |
Date |
Country |
Kind |
3471/81 |
Nov 1981 |
HUX |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation-in-part application of another application filed Nov. 18, 1982 and bearing application Ser. No. PCT/HU82/00061, published as WO83/01779, May 26, 1983. This claim is made under Section 35 U.S.C. 365 (c), under Section 35 U.S.C. 371 and under any other Section of the U.S.C. supporting such claim.
US Referenced Citations (5)
Foreign Referenced Citations (1)
Number |
Date |
Country |
8301779 |
May 1983 |
WOX |
Non-Patent Literature Citations (2)
Entry |
Staining Methods Histologic and Histochemical, McManus et al., eds., Paul B. Hoeber, Inc., N.Y., 1960, p. 237. |
H. J. Conn's Biological Stains, 9th Ed., Lillie, Ed., Williams & Wilkins Co., Baltimore, 1977, pp. 257-266; 328, 385-387; 397-400; 417-421, 428-429; 453-455. |