Claims
- 1. A method of enriching a solution for an adenovirus comprising contacting a solution containing an adenovirus with an anion exchange chromatography resin comprising an acrylate or sulphonamide linker such that the adenovirus binds to the chromatography resin and eluting the adenovirus from the resin with an eluant to obtain an enriched solution of adenovirus.
- 2. The method of claim 1, wherein the anion exchange chromatography resin comprises a quaternary amine binding moiety.
- 3. The method of claim 1, wherein the anion exchange chromatography resin comprises a tertiary amine binding moiety.
- 4. The method of claim 1, wherein the anion exchange chromatography resin is an expanded bed adsorption resin.
- 5. The method of claim 4, wherein the expanded bed adsorption resin is characterized by having a bead size of about 30-500 μm in diameter.
- 6. The method of claim 1, wherein the method comprises subjecting a solution containing an adenovirus to tangential flow filtration to produce the solution contacted with the anion exchange chromatography resin.
CROSS-REFERENCE TO RELATED PATENT APPLICATIONS
[0001] This patent application is a divisional of copending U.S. patent application Ser. No. 09/997,909, filed Nov. 30, 2001, which is a continuation of U.S. patent application Ser. No. 09/296,962, filed Apr. 22, 1999, now U.S. Pat. No. 6,383,795, which claims the benefit of U.S. Provisional Patent Application No. 06/082,628, filed Apr. 22, 1998.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60082628 |
Apr 1998 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09997909 |
Nov 2001 |
US |
Child |
10429303 |
May 2003 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09296962 |
Apr 1999 |
US |
Child |
09997909 |
Nov 2001 |
US |