Patent Application
20220099619
The subject matter relates to gel electrophoretic processes, and more particularly, to an electrode and an application of the electrode in the processes.
During electrophoresis, a pH electrode and an electrophoresis electrode are needed. The pH electrode is used to detect a pH value of a buffer. The electrophoresis electrode is used to electrically connect the electrophoresis detection device to an external power supply. However, the two kinds of electrodes increase the cost of the electrophoresis detection device. Therefore, there is room for improvement in the art.
Implementations of the present technology will now be described, by way of example only, with reference to the attached figures.
It will be appreciated that for simplicity and clarity of illustration, where appropriate, reference numerals have been repeated among the different figures to indicate corresponding or analogous components. In addition, numerous specific details are set forth in order to provide a thorough understanding of the embodiments described herein. However, it will be understood by those of ordinary skill in the art that the embodiments described herein can be practiced without these specific details. In other instances, methods, procedures, and components have not been described in detail so as not to obscure the related relevant feature being described. Also, the description is not to be considered as limiting the scope of the embodiments described herein. The drawings are not necessarily to scale, and the proportions of certain parts may be exaggerated to better illustrate details and features of the present disclosure.
The term “comprising,” when utilized, means “including, but not necessarily limited to”; it specifically indicates open-ended inclusion or membership in the so-described combination, group, series, and the like.
During an electrophoresis process, a pH value of a buffer in which a gel medium is immersed needs to be detected by a pH electrode. Different pH values mean that mobility of charged ions in the buffer is also different. At the same time, an electrophoresis electrode is also used to electrically connect the electrophoresis detection device to a power supply. The existing pH electrode may be a glass platinum electrode, which includes a glass base layer and a metal layer on the base layer. The metal layer is coated on a surface of the base layer by physical vapor deposition (PVD). The metal layer may be made of titanium (Ti), platinum (Pt), and a combination thereof. However, the glass platinum electrode is fragile and does not have flexibility. Once the glass platinum electrode is manufactured, a specification and a shape of the glass platinum electrode are fixed, which does not meet all different needs. That is, the glass platinum electrode has a poor adaptability. Moreover, the PVD technology needs vacuum pumping, and the metal layer is made of precious metals, which increases the cost of the glass platinum electrode. The electrophoresis electrode is also formed by PVD technology, which includes a substrate (such as a metal substrate or a non-conductive substrate) and a Ti/Pt composite metal coating deposited on the substrate. However, the cost of the electrophoresis electrode is also high.
In an embodiment, the metal wire 1 may be made of stainless steel. The stainless steel has a low cost, which reduces the cost of the pH electrode 10.
In an embodiment, the stainless steel may be, but is not limited to, SUS 304, SUS 312, and SUS 316.
In an embodiment, the stainless steel may be SUS 304.
In an embodiment, a diameter of the pH electrode 10 may be, but is not limited to, 0.25 mm, 0.3 mm, 0.48 mm, 0.5 mm, and 0.8 mm. The diameter of the pH electrode 10 cannot be too large, which increases a resistance of the pH electrode 10 and affects the accuracy of the pH value measurement. In an embodiment, the diameter of the pH electrode 10 may be 0.3 mm.
Referring to
In an embodiment, the surface treatment layer 2 may be made of a metal selected from a group consisting of zinc (Zn), tin (Sn), nickel (Ni), chromium (Cr), and any combination thereof.
The pH electrode 10 is made of Ti wire. The diameter of the pH electrode 10 is about 0.25 mm.
The pH electrode 10 is made of stainless steel. The stainless steel is SUS 304. The diameter of the pH electrode 10 is about 0.3 mm.
The pH electrode is the commercial glass platinum electrode.
The electrodes of EXAMPLES 1-2 and the COMPARATIVE EXAMPLE are used to measure the pH value of a buffer and a standard liquid. The measurement can be carried out by placing a buffer or a standard liquid in an electrophoresis groove of the electrophoresis detection device. Then, each electrode is immersed in the buffer and the standard liquid to measure the pH values of the buffer and the standard liquid.
In an embodiment, a composition of the buffer includes a tris (Tris(hydroxymethyl)aminomethane, 242 g/L), an acetic acid (58.1 g/L), and an EDTA (18.6 g/L).
The pH values measured by the three different electrodes are shown in Table 1.
From Table 1, the pH values measured by the pH electrodes 10 in EXAMPLE 1 and in EXAMPLE 2 are similar to the pH value measured by the glass platinum electrode in COMPARATIVE EXAMPLE. In particular, the pH value measured by the pH electrode 10 of EXAMPLE 2 is closer to the pH value measured by the glass platinum electrode. Thus, the accuracy of the pH electrode 10 is similar to that of the glass platinum electrode.
The pH electrode 10, especially the pH electrode 10 made of stainless steel, may be used in a weakly alkaline test liquid, temperature of the environment not exceeding 80 □. Under the above conditions, the pH electrode 10 made of stainless steel will not be oxidized, and the accuracy of the pH electrode 10 is similar to that of the existing glass platinum electrode. Therefore, the pH electrode 10 is applicable for the miniature electrophoresis detection device, which is used in a portable nucleic acid detection device. The pH value of the buffer in the miniature electrophoresis detection device is between 7 and 9, which is weakly alkaline, and the temperature of the measuring environmental condition is below 80 □. Therefore, it is possible to replace the existing glass platinum electrode with the pH electrode 10 made of the stainless steel, which reduces the cost of the pH electrode 10.
During the detection of the pH value, one of the two pH electrodes 10 is immersed into the buffer to detect the pH value of the buffer, and another one of the two pH electrodes 10 is immersed into the standard liquid to detect the pH value of the standard liquid. The pH value of the buffer is obtained by calculating a potential difference between the buffer and the standard liquid.
In an embodiment, a surface treatment layer 4 is coated on a surface of the metal wire 3. The material of the metal wire 3 is the same as that of the metal wire 1 of the pH electrode 10, and the material of the surface treatment layer 4 is same as that of the surface treatment layer 2 of the pH electrode 10.
The electrophoresis electrode 20 has flexibility and may be in diverse sizes. Thus, the electrophoresis electrode 20 can be used in different electrophoresis detection devices with different specifications. The electrophoresis electrode 20 has a low cost, and the preparation process is simple, which is especially suitable for the miniature electrophoresis detection device.
In an embodiment, the electrophoresis body 30 includes a base board 11, a plurality of sidewalls 12 connected to the base board 11, and a cover plate (not shown) disposed on ends of the sidewalls 12 away from the base board 11. The base board 11, the sidewalls 12, and the cover plate cooperatively form the electrophoresis groove 15.
In an embodiment, one of the sidewalls 12 defines two through holes 13. The through holes 13 are disposed on the ends of the one of the sidewalls 12. The two electrophoresis electrodes 20 extend into the buffer 40 through the two through holes 13.
In an embodiment, a fixing member 14 is disposed on an outside surface of each through hole 13. The fixing members 14 are fixed on the sidewall 12 to correspond to the through holes 13. Each of the electrophoresis electrodes 20 passes through one of the through holes 13, and is fixed through the fixing member 14 corresponding to the through hole 13. Thus, the electrophoresis electrodes 20 do not shake or move on the electrophoresis body 30, thereby achieving a stable electrical connection between the electrophoresis detection device 200 and the power supply.
The electrophoresis electrode 20 can be applied in the existing electrophoresis detection device. The electrophoresis electrode 20 can be installed in place of the existing electrode. Thereby, the electrophoresis electrode 20 can reduce the cost of the existing electrophoresis detection device. Moreover, the electrophoresis electrode 20 has a simple structure, which is convenient to install, be bent freely, and adaptable to a variety of applications.
The detection chip 302 and the electrophoresis detection device 200 are connected and are disposed in the kit body 301. The detection chip 302 and the electrophoresis detection device 200 are electrically connected to the connector 303. The detection chip 302 is used to perform a PCR amplification reaction. The electrophoresis box 3 is used to perform an electrophoresis process. A sample for testing undergoes the PCR amplification in the detection chip 302 to obtain a mixer. The mixer enters the electrophoresis detection device 200 for the electrophoresis process. An image collection unit (not shown) acquires a fluorescent image of the electrophoresis detection device 200. The nucleic acid detection kit 300 integrates with the detection chip 302 and the electrophoresis detection device 200, which has a small size, and is suitable for a portable use. After the PCR amplification, the electrophoresis detection can be carried out automatically. The two processes are performed in a single equipment, and the sampling accuracy is controlled accurately. Thus, the detection process is efficient and flexible.
In an embodiment, the nucleic acid detection kit 300 further includes a heating circuit board 304 disposed on a first surface of the detection chip 302. The heating circuit board 304 is electrically connected to the connector 303. The heating circuit board 304 is configured to heat the detection chip 302. One end of each electrophoresis electrode 20 of the electrophoresis detection device 200 away from the electrophoresis body 30 is electrically connected to the heating circuit board 304 to electrically connect the electrophoresis detection device 200 and the connector 303. By directly connecting the electrophoresis electrodes 20 to the heating circuit board 304, the nucleic acid detection kit 300 has a simple structure and is easy to assemble.
In an embodiment, two buckles 305 are disposed on the heating circuit board 304. The buckles 305 clamp the electrophoresis electrodes 20, achieving a stable electrical connection between the electrophoresis detection device 200 and the heating circuit board 304.
With the above configuration, an electrode provided by the present disclosure can be used in multiple applications. The electrode can be used as the pH electrode 10 of the electrophoresis detection device and can be used as the electrophoresis electrode 20 of the electrophoresis detection device. The pH electrode 10 and the electrophoresis electrode 20 can be the same electrode, which reduces the cost of the electrophoresis detection device. In addition, the electrode which is used for the pH electrode 10 and the electrophoresis electrode 20 is made of metal wire, which has simple structure and good flexibility, and can be bent according to actual need.
The embodiments shown and described above are only examples. Even though numerous characteristics and advantages of the present technology have been set forth in the foregoing description, together with details of the structure and function of the present disclosure, the disclosure is illustrative only, and changes may be made in the detail, including in matters of shape, size and arrangement of the parts within the principles of the present disclosure, up to and including, the full extent established by the broad general meaning of the terms used in the claims.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202110725615.0 | Jun 2021 | CN | national |
| Number | Date | Country | |
|---|---|---|---|
| 63085368 | Sep 2020 | US | |
| 63085385 | Sep 2020 | US | |
| 63138980 | Jan 2021 | US |
