1. Field of Invention
The present invention relates generally to a magnetic stirring apparatus.
2. Description of Related Art
The fields of biology and chemistry require the stirring of liquids and suspensions in target vials prior to and during sampling. Accordingly, a variety of stirring devices have been developed over the years, several of which were used in conjunction with autosamplers. Some previous devices utilized magnets mounted on motors that moved in space around or under the target vials. However, the motors only had an operating life of about 2000 hours, which made such designs unreliable for use with an autosampler that operates upwards of 8000 hours a year. Further, the placement of magnets between the target vials and the size of the magnets required to achieve active coupling with the stir bars decreased the number of vials that could be placed in a given area.
Other devices drove multiple stir bar locations simultaneously by placing pole shoes on top of coils and utilizing the pole shoes to direct a shared magnetic field under multiple vials placed in close proximity to one another. However, these pole shoe devices did not provide an acceptable consistent stirring action because the strength vector of the shared magnetic field became distorted by the magnetic stir bars placed in the vials. The distorted magnetic field resulted in the application of a strong magnetic field to the stir bars in some vials, and a weak magnetic field to the stir bars in other vials.
Accordingly, a need exists for a reliable stirring device that is capable of stirring samples contained in target vials placed in close proximity to one another and applies an individual and consistent magnetic field strength vector to the stir bar in each vial. The present invention addresses one or more of these needs.
The stirring apparatus is an electronic design that generates rotating magnetic fields to drive magnetic stir bars within vials placed above the cover of the stirring apparatus. The stir bar in each vial is driven by a separate magnetic field. The cover acts as a vial seating plane. Below the cover is a magnetics board containing multiple vial groups of air core coils. The cover contains an array of pole standoffs that matches the coil pattern. The hollow center of each air core coil contains at least a portion of one pole standoff. The heads of the pole standoffs extend through the cover and are flush with the top surface of the cover.
Embodiments of this invention contain one or more of these and other features and advantages described in, or made apparent from, the above summary of inventive features and the following detailed description of various exemplary embodiments of this invention.
The structure, operation, and advantages of the presently disclosed embodiment of the invention will become apparent when consideration of the following description taken in conjunction with the accompanying drawings wherein:
The invention will now be described in the following detailed description with reference to the drawings, wherein preferred embodiments are described in detail to enable practice of the invention. Although the invention is described with reference to these specific preferred embodiments, it will be understood that the invention is not limited to these preferred embodiments. But to the contrary, the invention includes numerous alternatives, modifications and equivalents as will become apparent from consideration of the following detailed description.
Referring now to the drawings, the stirring apparatus 10 of
Oscillator 59 is a simple comparator circuit that utilizes a resistor, capacitor and potentiometer 58 to set the oscillation frequency. In the preferred embodiment, the frequency can be adjusted between 300-1280 Hz, which translates to a stirring frequency between 280-1200 RPM.
In the preferred embodiment, stepper driver 60 of driver board 50 is configured to produce a micro-step output at 1/16 step, which results in two phases of pseudo-sine waves 90° out of phase from one another. When compared to an output of ½ step, a micro-step output of 1/16 step improves the stirring action and maintains a consistent magnetic field strength vector throughout the field rotation because the 1/16 step increments decrease the lead between the new field position and stir bar after indexing. Further, the Vref of stepper driver 60 is set for a peak current of 1.33 A, which limits the overall power of the preferred embodiment to about 15 W. Additionally, the input voltage of PFD (percentage fast decay) is fixed at 0.37 VDD, which switches the output current decay mote to the mixed decay mode when a STEP input signal commands a lower output current than the previous step.
In the preferred embodiment, linear regulator 54 is part number MAX1615 from Maxim Integrated Products; fan power linear regulator 55 is part number LM317 from National Semiconductor Corporation; oscillator 59 is part number LTC1440 from Linear Technology Corporation; stepper driver 60 is part number A3979SLP-T from Allegro MicroSystems, Inc.; and buffer driver 61 is part number 74LVC1G14 from Texas Instruments Incorporated. The datasheets for the part numbers listed in this paragraph are hereby incorporated by reference.
Magnetics board 30 of
In the preferred embodiment, coils 32 in the vial groups are arranged in a square pattern with 0.6 inch sides. The coils 32 are centered on the vertices of the square pattern. The vial groups are organized in a three by seven matrix on a printed circuit board. Further, in the preferred embodiment, coils 32 are part number 1159W1-RF from Triad Magnetics.
Coils 32 are driven by the two phases of stepper driver 60 (phase 1 and phase 2). Accordingly, each vial group of four coils 32 has one pair of coils 32 wound in opposite directions and wired in series to phase 1, and a second pair of coils 32 wound in opposite directions and wired in series to phase 2. In each vial group, the phase 1 coil pair and phase 2 coil pair are diagonal from one another. Therefore, when current flows through a pair of coils, the diagonal coils produce magnetic fields oriented in opposite directions.
The coils 32 are wired to create multiple sets of parallel coils in series for each phase of electrical power. In the preferred embodiment, each phase has 14 sets of three parallel coils 32 connected in series. This configuration ensures that the loss of a single coil 32 will not cause diminished stirring capability in more than one position. Further, tests have shown that the preferred embodiment can effectively stir a vial when only three of the four coils 32 are functioning in a vial group.
The adjacent (directly opposing) coils 32 of adjacent vial groups are driven by the same phase and have the same magnetic direction, which minimizes the corruption of the magnetic field produced by adjacent vial groups. This is illustrated in
If the adjacent (directly opposing) coils 32 of adjacent vial groups are not driven by the same phase and do not have the same magnetic direction, an adjacent (directly opposing) coil 32 from a first adjacent vial group could incorrectly couple with an adjacent (directly opposing) coil 32 from a second adjacent vial group, instead of correctly coupling with its paired coil 32 driven by the same phase in the first vial group. This incorrect coupling would corrupt the magnetic field produced by each vial group and disrupt stirring.
The likelihood of incorrect coupling increases if the distance between the adjacent (directly opposing) coils 32 of adjacent vial groups is less than the distance between the diagonal coil pairs. As can be seen in
Cover 20 of
The heads 21b of pole standoffs 21 extend through cover 20 and are flush with the top surface 20a of cover 20, thereby placing the magnetic poles on the top surface 20a of cover 20. Moving the poles from the top 32a of coils 32 to the top surface 20a of cover 20 resulted in a 100% increase in field strength at the top surface 20a of cover 20. In some embodiments, pole standoffs 21 extend through the entire length of coils 32. A variety of components can be used as pole standoffs 21, including, but not limited to, threaded standoffs or unthreaded studs. In some embodiments, pole standoffs 21 are threaded 1 inch carbon steel PEM self clinching standoffs from PennEngineering, part number BSO-6440-24. In other embodiments, pole standoffs 21 are unthreaded carbon steel PEM self clinching studs from PennEngineering, part number FH-215-24ZI. In the preferred embodiment, cover 20 is aluminum.
Some embodiments of magnetic stirring apparatus 10 include coupling plane 40, one embodiment of which is depicted in
Embodiments that include coupling plane 40 also have apertures 33 under each coil 32 of magnetics board 30. These apertures 33 allow coupling standoffs 41 or pole standoffs 21 to pass through magnetics board 30. In some embodiments, coupling plane 40 contains an array of coupling standoffs 41 that are of sufficient length and spaced such that the hollow center of each air core coil 32 on magnetics board 30 contains a portion of one coupling standoff 41. Coupling standoffs 41 act as pseudo-cores for air core coils 32.
In some embodiments that utilize coupling plane 40 with coupling standoffs 41, the distal end 41a of coupling standoffs 41 contact the distal end 21a of pole standoffs 21. In other embodiments that utilize coupling plane 40 with coupling standoffs 41, there is a gap between the distal end 21a of pole standoffs 21 and the distal end 41a of coupling standoffs 41.
A variety of components can be used as coupling standoffs 41, including, but not limited to, threaded standoffs or non-threaded studs. In some embodiments, coupling plane 40 is steel and coupling standoffs 41 are carbon steel PEM self clinching standoffs from PennEngineering, part number BSO-6440-16.
In some embodiments, coupling plane 40 is a sheet of steel and does not contain an array of coupling standoffs 41. In some embodiments that utilize a coupling plane 40 without coupling standoffs 41, the distal end 21a of pole standoffs 21 extend through the hollow center of each air core coil 32 and contact coupling plane 40. In other embodiments that utilize a coupling plane 40 without coupling standoffs 41, there is a gap between the distal end 21a of pole standoffs 21 and coupling plane 40.
The fully assembled preferred embodiment is shown in
In some variations of this embodiment, the distal end 21a of pole standoffs 21 enter into coils 32 and contact the first leg 45b and second leg 45c of the first coupling piece 45, and the first leg 46b and second leg 46c of the second coupling piece 46. In other variations of this embodiment, when the distal end 21a of pole standoffs 21 enter into coils 32, a gap is present between the distal end 21a of pole standoffs 21 and the first leg 45b and second leg 45c of first coupling piece 45, and the first leg 46b and second leg 46c of second coupling piece 46.
A user operates stirring apparatus 10 as follows: first, a user can optionally configure the stirring circuitry settings by removing cover 20, manipulating the first configuration header 64 to enable or disable the rectification and sleep mode functions, and manipulating the second configuration header 65 to select the logic supply voltage, direction of the magnetic field rotation, and step size. A user can select a 1/16, ¼, ½ or full step size, which creates between 4 and 32 pole positions at intervals in each vial group of four coils 32. When a 1/16 step size is selected, 32 pole positions are created at intervals in each vial group.
After a user sets configuration headers 64 and 65, cover 20 is replaced and the 24 VDC power supply is connected to power supply connector 51. Next, a user places a vial rack containing target vials on cover 20. Index pins 22 position the vial rack on cover 20 such that each target vial is positioned above an individual vial group. Each vial group on magnetics board 30 generates an individual magnetic field that drives the magnetic stir bar contained in the target vial located above the vial group.
After the vial rack is in position, a user manipulates the switch in potentiometer 58 from the OFF position to the ON position, which allows power switch 52 to conduct current, thereby powering linear regulator 54, power LED 53, and fan power linear regulator 55. Linear regulator 54 supplies power to oscillator 59, potentiometer 58, and stepper driver 60. Stepper driver 60 is also supplied with 24 VDC, which is used to drive coils 32.
A user then adjusts the oscillation speed using potentiometer 58, which controls the output of oscillator 59 that is provided as an input to stepper driver 60. When stepper driver 60 receives an input from oscillator 59, stepper driver 60 advances the magnetic fields of the vial groups to the next pole position, thereby also advancing the coupled stir bars to the next pole position. Accordingly, when the step size is set to 1/16 (micro stepping mode) and potentiometer 58 is adjusted such that oscillator 59 produces an output of 300 Hz, the magnetic stir bars in target vials will spin at about 280 RPMs. Further, if the oscillator produces an output of 1280 Hz, the magnetic stir bars will spin at about 1200 RPMs.
This stirring apparatus 10 is well suited for use in conjunction with an unattended autosampler because micro stepping more effectively couples the stir bar to the pole location, thereby allowing for the use of smaller coils and less power. Further, since an individual magnetic field is provided at each vial location, a user can visually verify that the stir bar is synchronized with the magnetic field throughout the magnetic field's entire rotation before allowing the stirring apparatus 10 to operate unattended.
While this invention has been described in conjunction with the specific embodiments described above, it is evident that many alternatives, combinations, modifications and variations are apparent to those skilled in the art. Accordingly, the preferred embodiments of this invention, as set forth above are intended to be illustrative only, and not in a limiting sense. Various changes can be made without departing from the spirit and scope of this invention.
This application is a continuation application of and claims the priority filing benefit of U.S. patent application Ser. No. 12/540,899, filed Aug. 13, 2009, now U.S. Pat. No. 8,398,297, and entitled “Electromagnetic Stirring Apparatus”, which is herein incorporated by reference in its entirety.
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Number | Date | Country | |
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20130155803 A1 | Jun 2013 | US |
Number | Date | Country | |
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Parent | 12540899 | Aug 2009 | US |
Child | 13764526 | US |