This invention relates to an electronic skin (e-skin) patch. More particularly, the invention relates to an electronic skin patch for monitoring health conditions such as heart rate.
The long-term goal of health-related wearable electronics is that a patient's health data can be gathered in real time via body-worn wireless sensors, providing remote, continuous monitoring of human health. This device has the potential to drastically transform the way patients are diagnosed and treated. Among all the heavily research areas in wearable systems, smart textiles have attracted tremendous attention by integrating flexible electronics into daily outfits. Existing technologies rely on electrical or optical measurements. However, the performance of smart textiles is often limited by the gaps between a human body and the fabrics. Furthermore, the smart outfits are usually changed multiple times each week and they are not comfortable to wear during sleep. As a result, the collected subset of possible signals might not represent the actual conditions of the patients.
Systems with longer-term signal collection and minimum human intervention are therefore needed.
In at least one embodiment, the present invention provides a wearable electronic system that can be attached to a human body to sense physiological signals such as heartbeat. The patch material is made of protein biopolymers having excellent biocompatibility. The detection is based on optical sensors coupled to a data collection circuit. The measurement in this invention is based on optical sensors that can detect arterial blood oxygen level caused by heartbeats. The patch material is made of protein biopolymers while existing systems (wrist bands and smart watches) use synthetic polymers. The invention results in a smaller and lighter wearable device. The device is a patch on the skin so it is non-obtrusive. The protein biopolymer patch material has better biocompatibility and is more comfortable to wear in comparison with synthetic polymers.
The accompanying drawings, which are incorporated herein and constitute part of this specification, illustrate the presently preferred embodiments of the invention, and, together with the general description given above and the detailed description given below, serve to explain the features of the invention. In the drawings:
In the drawings, like numerals indicate like elements throughout. Certain terminology is used herein for convenience only and is not to be taken as a limitation on the present invention. The following describes preferred embodiments of the present invention. However, it should be understood, based on this disclosure, that the invention is not limited by the preferred embodiments described herein.
As disclosed herein, a number of ranges of values are provided. It is understood that each intervening value, to the tenth of the unit of the lower limit, unless the context clearly dictates otherwise, between the upper and lower limits of that range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in that stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range, and each range where either, neither, or both limits are included in the smaller ranges is also encompassed within the invention, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the invention. The term “about” generally refers to plus or minus 10% of the indicated number. For example, “about 10%” may indicate a range of 9% to 11%, and “about 20” may mean from 18 to 22. Other meanings of “about” may be apparent from the context, such as rounding off, so, for example “about 1” may also mean from 0.5 to 1.4.
E-Skin Patch
With reference to
With reference to
With reference to
Sensor and Electronic Components
In
In an example, the memory can be a non-transitory computer-readable memory that contains data and/or programming instructions. The programming instructions may be programmed to cause the microcontroller to receive sensor signals that are generated by the electronic health monitoring sensor 330, where the sensor signal is representative of a health condition of a patient. The microcontroller can also be programmed to generate health information about the patient based on the sensor signal, store the health information in the memory 44 and/or transmit the health information to a health monitoring system. In one example, the circuit may transmit the health information via I/O interface 49 to an external monitoring device that is connected to the sensor circuit by USB. In another example, the communication interface is a Bluetooth™ interface and the circuit may transmit the health information to a health monitoring system via Bluetooth™. Whether using a wired (e.g. USB) or wireless interface, the electronic skin patch may be periodically connected with an external health monitoring device to transfer health information thereto.
With reference to
In another example, the health monitoring sensor can be a piezoelectric sensor that generally converts vibration into electrical signals, and can be used to measure the movement of the skin to sense vibrations due to heartbeat and respiration, or other health conditions. Piezoelectric sensors may be made of thin film piezoresistor, nanomaterials, carbon nanotubes or nanofibers, to further reduce the size of the device and to provide greater sensitivity of the sensor. Because a piezoelectric sensor detects signals via vibrations, it requires the base member of the electronic skin patch (22 in
In one design consideration, the choice of electronic health monitoring sensor may be at least based on the signal-to-noise level of the sensor. For example, with reference to
In another design consideration, the electronic health monitoring sensor may be a piezoelectric sensor, if, for example, the transparency requirement of the base member of the patch associated with an optical sensor is high and cannot be readily satisfied. The issues with noise associated with piezoelectric sensors may be overcome via extensive signal processing. The processing can be achieved by the microcontroller of the electronic skin patch (e.g. 342 in
With reference to
With reference to
The sensor device may include an electronic health monitoring sensor that can generate a sensor signal representative of a health condition of a patient. The health monitoring sensor can be a piezoelectric sensor that generates sensor signals from vibrations on the skin of a patent, or an optical sensor, or other known or hereafter sensors that are suitable for measuring a health condition of a patient.
The sensor device will also include a circuit, including a controller, communicatively connected to the electronic health monitoring sensor and configured to receive the sensor signal and transmit health information based on the received sensor signal to the health monitoring device. In one example, the electronic skin patch 520 may have a Bluetooth™ interface and transmit health information to the health monitoring device 76 via Bluetooth™. Alternatively, and/or additionally, the health monitoring device 76 receives the health information from the electronic skin patch 520 and transmits the received health information to a remote mobile device 74 via the Internet 72. Alternatively, and/or additionally, the remote mobile device 74 may communicate directly with the electronic skin patch via Bluetooth™ or other wireless communication protocols. The health monitoring device or the remote mobile device may also be installable or accessible at a hospital room 78, by ambulance personnel 77 or by a treating physician 79.
Biopolymer Materials
The polymeric portion of the wearable electronic skin patch 20 (in
For skin patches the biopolymers are specially designed with the appropriate strength, flexibility, morphology and color to mimic human skin.
Such biopolymers are potentially more biocompatible with human skin than typical synthetic polymers such as latex rubber or vinyl polymers. Such biopolymers also show biodegradability in vivo, and as such can be resorbed by tissues, specifically human tissues.
The protein biopolymers can be plant-derived (for example, without limitation, corn zein and soy proteins or the like), can be insect-derived (for example, without limitation, silk from various species of silkworms, such as the Indian silkworms Antheraea mylitta (Tussah), Philosamia ricini (Eri), and Antheraea assamensis (Muga); the Thailand silkworm (Thai); and the Chinese mulberry silkworm Bombyx mori (B. mori)), or can be higher animal-derived (for example, without limitation, keratin from sheep or llama wool, or hair from other species, including human hair; and collagen or elastin from various tissues) or can include any combinations thereof including synthetic or natural biopolymers. These biomaterials have different protein secondary structures: e.g., alpha-helix structures for zeins, variable beta-sheet structures for silks, and coiled-coils structures for keratins. Therefore, the mechanical properties of the different proteins can be different.
In one embodiment of the invention about 0.5 to about 50 wt % (about 1 to about 45 wt %; about 5 to about 40 wt %; about 10 to about 35 wt %; about 15 to about 30 wt %; about 20 to about 25 wt %; about 10 to about 25 wt %; about 10 to about 30 wt %; about 10 to about 40 wt %; about 10 to about 45 wt %; about 10 to about 50 wt %; about 15 to about 20 wt %; about 15 to about 25 wt %; about 15 to about 35 wt %; about 15 to about 40 wt %; about 15 to about 45 wt %; about 15 to about 50 wt %; about 20 to about 30 wt %; about 20 to about 35 wt %; about 20 to about 40 wt %; about 20 to about 45 wt %; about 20 to about 50 wt %; about 25 to about 30 wt %; about 25 to about 35 wt %; about 25 to about 40 wt %; about 25 to about 45 wt %; about 25 to about 50 wt %; about 30 to about 35 wt %; about 30 to about 40 wt %; about 30 to about 45 wt %; about 30 to about 50 wt %; about 35 to about 40 wt %; about 35 to about 45 wt %; about 35 to about 50 wt %; about 40 to about 45 wt %; about 40 to about 50 wt %; about 0.5 to about 3.0 wt %; or about 0.5 to about 1.5 wt %; or about 0.5 to about 1.0 wt %; or 0.5 wt %; or 1.0 wt %; or 1.5 wt %; or 2.0 wt %; or 2.5 wt %; or 3.0 wt %; or 3.5 wt %; or 4.0 wt %; or 4.5 wt %; or 5.0 wt %) solutions of wild tussah (Antheraea mylitta) silk protein or domesticated mulberry (Bombyx mori) silk protein were separately prepared and was cast onto polydimethylsiloxane (PDMS) or glass substrates to form films. Preferably about 1.0 wt % silk protein solutions were prepared and combined. After drying, the films were removed from the PDMS or glass.
One embodiment of the biopolymer comprises a composite of two or more individual biopolymers. This can be a composite of two or more biopolymers from the same class, such as a mixture of polyhydroxyacids, for example a mixture of PGA and PLA. The composite can also be an alloy of proteins or peptides, such as a mixture of B. mori silk and sheep wool keratin. The composite can also be a mixture of two or more biopolymers from different classes, such as a mixture of B. mori silk and PLA, for example. The ability to readily adjust the composition of the biopolymer allows one to control various tunable biophysical properties of the biopolymer film, and consequently, of the e-skin patch. Such tunable properties include flexibility, elasticity, strength, surface roughness and surface charge.
These proteins can be modified by methods known in organic chemistry to incorporate or remove certain functional groups. In some embodiments the proteins are degummed to remove undesirable components (e.g. soluble silk sericin proteins coated on most silk fibroin fibers) using procedures known in the art (e.g. Rockwood, D. N. et al. Materials fabrication from Bombyx mori silk fibroin, Nat. Protocols. 6, 1612-1631 (2011)).
The protein can be natural, synthetic or recombinant or a combination thereof. For example, the protein can be a natural or recombinant silk protein from different silkworm species.
In some embodiments, two or more proteins are dissolved in a solution to prepare a protein alloy biomaterial. For example, a protein from wild tussah silk and a protein from domesticated mulberry silk can be dissolved in the same solution and processed according to methods of the present invention to obtain a biomaterial of unique mechanical properties. The ratio of two proteins can be about 95:5 to 5:95, or about 90:10 to 10:90, or about 85:15 to 15:85, or about 80:20 to 20:80, or about 75:25 to 25:75, or about 70:30 to 30:70, or about 65:35 to 35:65, or about 60:40 to 40:60, or about 55:45 to 45:55, or about 50:50.
The processed protein exhibits significant differences in properties versus unprocessed protein. For example, a higher crystallinity and/or more organized crystal alignment can be observed in processed protein film. The β-sheet (B) contents in protein films can also be increased from about 1 to about 70% (about 5 to about 60%; or about 10 to about 50%; or about 15 to about 40%; or about 20 to about 35%; or 2%; or 3%; or 4%, etc. up to 70%) after processing. Further, the processed protein may become completely insoluble in water, in contrast to being partially soluble prior to being processed. Other properties including solvent evaporation temperature Td1, degradation temperature Td2, and absorbance may also be changed by processing. In some embodiments, an IR absorbance peak of the processed protein shows a red shift to lower frequency (e.g. 1640 cm−1 shifted to 1620 cm−1). In some embodiments, only a single absorbance peak shows red shift to lower frequency.
The cast silk films tended to be somewhat stiff and brittle in the dry state. However, the e-skin patch is expected to be used in various environments, with high or low humidity levels. Therefore, about 10 to about 35 wt % (based on the weight of the protein component) of one or more environmentally friendly (“green”) biocompatible plasticizers, such as glycerol (glycerin), was blended into the liquid protein compositions so that the cast films can maintain flexibility under different environmental conditions. In one embodiment, the flexibility of the resulting film containing 20 wt % of glycerol was observed to be dramatically increased.
In one embodiment the total amount of plasticizers in the liquid protein composition is about 10 to about 35 wt % versus the protein material; in another embodiment one or more plasticizers are present in a total of about 10 to about 25 wt % versus the protein material; in another embodiment one or more plasticizers are present in a total of about 10 to about 20 wt % versus the protein material; in another embodiment one or more plasticizers are present in a total of about 15 to about 25 wt % versus the protein material. In a preferred embodiment the total amount of plasticizer in the liquid protein composition is about 20 wt % versus the protein material. In other words, the weight ratio of total protein to total plasticizer is about 90:10 to about 65:35; or about 90:10 to about 75:25; or about 90:10 to about 80:20; or about 85:15 to about 75:25. These same ratios apply to the cast films. Without limitation, the biocompatible plasticizers can be selected from sorbitan, sorbitan anhydrides, castor oil, diacetylated monoglycerides, mono- and di-acetylated monoglycerides, triacetin (glycerin triacetate), glycerol (glycerin), erythritol, threitol, arabitol, xylitol, ribitol, mannitol, sorbitol, galactitol, fucitol, iditol, inositol, volemitol, isomalt, maltitol, lactitol, maltotriitol, maltotetraitol, polyglycitol, polyvinyl alcohol, propylene glycol, triethyl citrate, tributyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, n-butyryl tri-n-hexyl citrate, oleic acid, steric acid, polyethylene glycols, and mixtures of two or more thereof.
The properties of the pure and modified silk film were investigated using various methods. For example, their structures were examined using Fourier transform infrared spectrometry (FTIR, Bruker Tensor 27) and their thermal properties were obtained using temperature-modulated differential scanning calorimetry (TMDSC).
The differential scanning calorimetry (DSC) results can prove the homogeneity of the materials. If the composite is not homogeneous, it will demonstrate both silk composite and pure silk thermal features in the DSC curves indicated by two glass transitions, two melting or degradation peaks. For the inventive composites only one set of glass transition and degradation peaks are observed, which are different from those in the pure silk sample. Therefore, the composite samples are homogeneous, and do not separate into composite and pure silk regions.
Thus the thermal properties of the SF film and SF-glycerol film samples were examined by standard DSC and temperature modulated DSC (TMDSC).
Temperature modulated DSC was used to measure the reversing thermal properties of the Mori SF film and Mori SF-glycerol film.
The mechanical properties of silk fibroin, silk-glycerol, and PDMS (as a control substrate) were examined. Using a mechanical tensile testing system the stress-strain data of the three materials were obtained and plotted into one graph for direct comparison, as shown in
The silk-glycerol composite sample demonstrates a balanced mechanical behavior in terms of flexibility and strength. The stress-strain curve (the middle curve) of the silk-glycerol composite is between the curves of PDMS and pure silk. It proves that the silk-glycerol composite can survive larger forces and greater elongation without breaking. The silk-glycerol composite is a much softer material compared with the pure silk films. Its mechanical properties make it more suitable for wearable applications. The key parameters are calculated as: Young's modulus of 1.16 MPa, ultimate strength of 2.67 MPa, and ultimate strain of 13.64. Desirably, the Young's modulus should lie in the range 0.26 to 141.11 MPa, the ultimate strength in the range 2 to 36.5 MPa and the ultimate strain before breaking in the range 10 to 71 MPa.
The surfaces and cross-sections of the silk, silk-glycerol composite, and PDMS (reference) films were observed with a scanning electron microscope (SEM). The images disclose the surface microstructures and morphologies of the three materials. No significant changes were found along the depth of the film, indicating homogeneous gel formation. The SEM images of the reference showed a pristine PDMS cross-linked microstructure. PDMS presented no significant change in morphology. The cross-sectional images of all three samples, pure SF, SF-glycerol composite, and PDMS, showed continuous and homogeneous structures without voids. The rough cross-sections indicated the tenacity fracture of the films, which is related to strong mechanical properties.
Integration
Integration of Protein Materials with Circuits:
The possibility of integrating protein materials with circuits was explored. Ultimately the current breadboard circuit will be miniaturized onto a small PCB circuit, and will be packaged into protein materials. Initial experiments directed to protein-PCB-protein integration were successful, as shown in
Integration of Protein Materials with Sensors:
The color of the silk and light transmission properties have an effect on how a PPG sensor reads a pulse. The table in
The effect of protein patches on sensors was also tested. The results can help determine a suitable silk material with the desired optical properties. They also help quantify the sensor performance in a silk-packaged system.
Miniaturization of Silk-Based Electronics:
Wearable electronics must be smaller and more portable than in the past. Therefore, further miniaturization of the silk-based e-skin patches is desirable. In one miniaturization embodiment silk substrates have been combined with nanofibers of the functional polymer polyvinylidene difluoride (PVDF), which can convert mechanical vibration into electricity (i.e., serve as a piezoelectric layer) as well as provide flexible support to the patch. A fully flexible device can be created using a pre-cured silk piece as the substrate and liquid silk as the top cover.
Adhesion with Skin-Friendly Adhesives:
The biopolymer-based patches can be adhered to human skin using skin-friendly adhesives. Suitable examples of such adhesives include, without limitation, acrylic-based adhesives, polyisobutylene-based adhesives and silicone-based adhesives, polyesters or polyurethane-based adhesives. One preferred adhesive is a pressure sensitive acrylate adhesive. Another preferred adhesive is a low-irritation adhesive for sensitive skin.
In certain embodiments the adhesives are also used in fabricating the e-skin patch, for example by layering a biopolymer film, the electronic component and another biopolymer film, with skin-friendly adhesive between these layers to form a sandwich-type patch. In another embodiment, the e-skin patch can have a backing layer of suitable skin-friendly adhesive material that minimizes cutaneous skin reaction, such as a pressure sensitive acrylate adhesive, and is configured such that trauma to the patient skin can be minimized while the patch is worn for the extended period of time (as used herein to include 24, 48, 72 hours or more and up to 120 days). For example, the base member 22 and the top substrate 26 (
In another embodiment the electronics can be coated with a water proof material, for example a sealant adhesive such as epoxy or silicone. In some embodiments, the e-skin patch can comprise a medicated patch that releases a medicament, such as antibiotic, beta-blocker, ACE inhibitor, diuretic, or steroid to reduce skin irritation. The e-skin patch can comprise a thin, flexible patch with a polymer grid for stiffening. This grid can be anisotropic and can utilize electronic components to act as a stiffener.
One aspect of the invention is directed to a wearable electronic skin patch for health monitoring, comprising: a top protein substrate; a base member having an upper surface and a lower surface with the lower surface having a skin-adhering adhesive thereon; and a sensor device sandwiched between the top protein substrate and the base member, the sensor device comprising: an electronic health monitoring sensor configured to generate a sensor signal representative of a health condition of a patient, and a circuit, including a controller, communicatively connected to the electronic health monitoring sensor and configured to receive the sensor signal and transmit health information to a health monitoring system; where the electronic skin patch is attachable to skin of the patient continuously for an extended period of time, and where the base member and the top protein substrate each independently comprise a plasticized protein composite film comprising: a) at least one naturally occurring biocompatible protein material selected from the group consisting silks and alloys thereof; and b) at least one biocompatible plasticizer; where the plasticizer is present in about 10% to about 35% by weight. In one embodiment of the electronic skin patch the electronic health monitoring sensor is a photoplethysmography (PPG) photo sensor configured to generate the sensor signal based on the rate of blood flow of the patient. In one embodiment of the electronic skin patch the base member is optically transparent to the wavelengths of light utilized by the PPG. In one embodiment the sensor device further comprises a battery; another embodiment further comprises a charging circuit configured to charge the battery via an external battery charger. In one embodiment the sensor device further comprises a memory configured to store a history of sensor data based on the sensor signals. In another embodiment the sensor device further comprises a wireless communication interface communicatively connected to the circuit and configured to transmit the health information to the health monitoring system wirelessly.
Another aspect of the invention is directed to a health monitoring system, comprising: a health monitoring device comprising: a processor, a display communicatively connected to the processor, a communication interface communicatively connected to the processor, and a computer-readable medium containing programming instructions configured to cause the processor to receive health information from a wearable electronic skin patch via a communication network and display information about a patient's health condition based on the received health information; where the wearable electronic skin patch comprises: a top protein substrate; a base member having an upper surface and a lower surface with the lower surface having a skin-adhering adhesive thereon; and a sensor device sandwiched between the top protein substrate and the base member, the sensor device comprising: an electronic health monitoring sensor configured to generate a sensor signal representative of a health condition of a patient, and a circuit, including a controller, communicatively connected to the electronic health monitoring sensor and configured to receive the sensor signal and transmit health information to the health monitoring device; where the electronic skin patch is attachable to skin of the patient continuously for an extended period of time, and where the base member and the top protein substrate each independently comprise a plasticized protein composite film comprising: a) at least one naturally occurring biocompatible protein material selected from the group consisting silks and alloys thereof; and b) at least one biocompatible plasticizer; where the plasticizer is present in about 10% to about 35% by weight. In one embodiment the electronic health monitoring sensor is a photoplethysmography (PPG) photo sensor configured to generate the sensor signal based on the rate of blood flow of the user. In one embodiment the sensor device further comprises an interface communicatively connected to the circuit and configured to transmit the health information to the health monitoring device wirelessly. In one embodiment the health monitoring device is configured to transmit information about the patient's health condition based on the received health information to a remote mobile device via a communication network.
Another aspect of the invention is directed to a method of fabricating a wearable electronic skin patch for health monitoring, comprising: providing a pre-cured silk substrate comprising: a) at least one naturally occurring biocompatible protein material selected from the group consisting silks and alloys thereof; and b) at least one biocompatible plasticizer; wherein the plasticizer is present in about 10% to about 35% by weight; depositing two or more electrodes on the silk substrate; collecting a PVDF nanostructure between the two or more deposited electrodes on the pre-cured silk substrate to provide a deposited PVDF nanostructure; depositing liquid PDMS or a liquid silk composition on the deposited PVDF nanostructure; and curing the liquid PDMS or silk composition; wherein said liquid silk composition comprises: a) at least one naturally occurring biocompatible silk material selected from the group consisting silks and alloys thereof; b) at least one biocompatible plasticizer selected from the group consisting of sorbitan, sorbitan anhydrides, castor oil, diacetylated monoglycerides, mono- and di-acetylated monoglycerides, triacetin (glycerin triacetate), glycerol (glycerin), erythritol, threitol, arabitol, xylitol, ribitol, mannitol, sorbitol, galactitol, fucitol, iditol, inositol, volemitol, isomalt, maltitol, lactitol, maltotriitol, maltotetraitol, polyglycitol, polyvinyl alcohol, propylene glycol, triethyl citrate, tributyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, n-butyryl tri-n-hexyl citrate, oleic acid, steric acid, polyethylene glycols, and mixtures of two or more thereof; and c) an aqueous solvent; where the plasticizer is present in the composition in about 10% to about 35% by weight versus the silk material.
Yet another aspect of the invention is directed to a liquid protein composition comprising: a) at least one naturally occurring biocompatible protein material selected from the group consisting of corn zein and silks; b) optionally a naturally occurring biocompatible non-protein material; c) at least one biocompatible plasticizer; and d) an aqueous solvent; where the composition is suitable for casting into films. In one embodiment of the liquid protein composition, the protein material is mori silk (from Bombyx mori). In another embodiment of the liquid protein composition, the biocompatible plasticizer is selected from the group consisting of sorbitan, sorbitan anhydrides, castor oil, diacetylated monoglycerides, mono- and di-acetylated monoglycerides, triacetin (glycerin triacetate), glycerol (glycerin), erythritol, threitol, arabitol, xylitol, ribitol, mannitol, sorbitol, galactitol, fucitol, iditol, inositol, volemitol, isomalt, maltitol, lactitol, maltotriitol, maltotetraitol, polyglycitol, polyvinyl alcohol, propylene glycol, triethyl citrate, tributyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, n-butyryl tri-n-hexyl citrate, oleic acid, steric acid, polyethylene glycols, and mixtures of two or more thereof, and is present in the composition in about 10% to about 35% by weight versus the protein material.
Another aspect of the invention is directed to a plasticized protein composite film cast from the liquid protein composition disclosed above.
Still another aspect of the invention is directed to a plasticized protein composite film comprising: a) at least one naturally occurring biocompatible protein material selected from the group consisting silks and alloys thereof; and b) at least one biocompatible plasticizer; where the plasticizer is present in about 10% to about 35% by weight. In one embodiment the plasticized protein composite film has a Young's modulus ranging from 0.26 to 141.11 MPa, an ultimate strength ranging from 2 to 36.5 MPa, and an ultimate strain ranging from 10 to 71 MPa. In another embodiment the plasticized protein composite film is characterized as having a homogeneous structure without voids. In another embodiment the plasticized protein composite film is optically transparent to the wavelengths of light utilized by a PPG sensor to be combined with said film to form a wearable electronic skin patch.
The method disclosed by Hu et al (Journal of Visualized Experiments, e50891, April 2014, pages 1-13) was used to prepare an alloy of wild tussah silk (Antheraea pernyi) and domestic mulberry silk (Bombyx mori). Briefly, solutions of wild tussah silk protein and domestic mulberry silk protein were prepared and combined in various ratios, which, after appropriate processing and drying, provided variable protein alloy materials useful as the biopolymer components of the e-skin patch.
Various materials were fabricated using this method and analyzed for their morphologies, structures, and properties, versus the synthetic polymer reference PDMS as a control.
Silk cocoons came from Bombyx Mori silkworm (China) were boiled for 25 min in an aqueous solution of 0.02 M NaHCO3 and rinsed thoroughly with Milli-Q water to remove the glue-like sericin proteins. The extracted silk proteins were dried and dissolved into a mixture solution of formic acid and calcium chloride (4 wt % of calcium chloride) for 15 min. After centrifugation and filtration to remove insoluble residues, a 6 wt % SF solution was obtained. To obtain the SF-glycerol solution, glycerol powders were directly added into the SF solution at the mass ratio of 1:4 (glycerol/silk). The final solutions (either SF or SF-glycerol composite) were casted onto pre-cured PDMS substrates to form films. After being rinsed in distilled water for 15 mins, the dried biocompatible SF or SF-glycerol films were obtained.
FTIR spectra of the pristine PDMS, pure SF, and SF-glycerol composite films in the range of 4000-450 cm−1 are shown in
Integration
Our initial trial on the integration was based on a wet pressing technology. The films were first treated by wet streams to soften the surface part of the protein samples, then a 100 N pressing was used on the edge sides of wet films to seal the two films together without using any glue materials. The films were then dried to form a stable two layer structure. Using this technique, a PCB board can be successfully incorporated into a two layer Mori silk films (
In the illustrated embodiment of
In one embodiment, with reference to
These and other advantages of the present invention will be apparent to those skilled in the art from the foregoing specification. Accordingly, it will be recognized by those skilled in the art that changes or modifications can be made to the above-described embodiments without departing from the broad inventive concepts of the invention. It should therefore be understood that this invention is not limited to the particular embodiments described herein, but is intended to include all changes and modifications that are within the scope and spirit of the invention as defined in the claims.
This application claims the benefit of priority under 35 USC §119(e) of U.S. Provisional Patent Application No. 62/233,741, filed on Sep. 28, 2015, the disclosure of which is incorporated herein by reference in its entirety.
Number | Date | Country | |
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62233741 | Sep 2015 | US |