Claims
- 1. A gel electrophoresis support comprising a selectively permeable polymer that is porous and non-wettable.
- 2. The support of claim 1 wherein the polymer is non-wettable in the absence of detergent or organic solvent and wettable in the presence of detergent or organic solvent.
- 3. The support of claim 1 wherein the polymer is comprised of polyethylene, polypropylene, polystyrene, polycarbonate or polysulfone.
- 4. The support of claim 1 wherein the polymer has porosity of 40-75% void fraction.
- 5. The support of claim 1 wherein the support has average pore dimensions of 0.05 to 1.0 μm.
- 6. The support of claim 2 wherein the detergent is comprised of sodium dodecyl sulfate.
- 7. The support of claim 1 wherein the support is comprised of a tube having two ends.
- 8. The support of claim 1 wherein the support is comprised of a container having a lower surface that is continuously connected to four sides.
- 9. A method of filling the support of claim 1 with an acrylamide monomer solution comprising insertion of the support into a polymer sheath having a length equal to or greater than the length of the support and a diameter slightly greater than that of the support and sufficient to allow insertion of the support; filling the support with catalyzed acrylamide monomer solution; allowing the monomer to polymerize and removing the filled support from the protective sheath.
- 10. A method for performing gel electrophoresis comprising;
a) providing a gel electrophoresis support comprising a selectively permeable polymer that is porous and non-wettable; b) applying a biological sample to one end of the support, wherein the support contains an acrylic polymer gel and is contained within a holder containing a conductive solution; and c) applying an electric current sufficient to cause components of the biological sample to migrate in the gel to form an electrophoresed sample.
- 11. The method of claim 10, wherein the support is comprised of a tube having two ends.
- 12. The method of claim 11, further comprising;
a) contacting the electrophoresed sample with a slab gel; wherein the slab gel is contained within a holder containing a conductive solution; and b) applying an electric current sufficient to cause the components of the electrophoresed sample to migrate into the slab gel.
- 13. The method of claim 10, wherein the support is comprised of a container having a lower surface that is continuously connected to four sides.
- 14. The method of claim 13, further comprising:
a) contacting the electrophoresed sample with a slab gel, wherein the slab gel is contained within a holder containing a conductive solution; and b) applying an electric current sufficient to cause the components of the electrophoresed sample to migrate into the slab gel.
- 15. A method to transfer components of a biological sample to a support medium comprising;
a) providing a gel electrophoresis support comprising a selectively permeable polymer that is porous and non-wettable; b) applying a biological sample to one end of the support, wherein the support contains an acrylic polymer gel and is contained within a holder containing a conductive solution; and c) applying an electric current sufficient to cause components of the biological sample to migrate in the gel to form an electrophoresed sample. d) contacting the electrophoresed sample with the support medium, wherein the electrophoresed sample and the support medium are contained within a holder containing a conductive solution; and e) applying an electric current sufficient to cause the components of the biological sample to migrate onto the support medium.
- 16. The method of claim 15, wherein the support is comprised of a tube having two ends.
- 17. The method of claim 15, wherein the support is comprised of a container having a lower surface that is continuously connected to four sides.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority based on U.S. Provisional Patent Application Serial No. 60/202,264, filed May 5, 2000, which is hereby incorporated by reference in its entirety.
Provisional Applications (1)
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Number |
Date |
Country |
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60202264 |
May 2000 |
US |