Patent Grant
8809212
The following application claims benefit of U.S. Provisional Application No. 61/280,875, filed Nov. 11, 2009, which is hereby incorporated by reference in its entirety.
Non-woven textiles formed from polymers are useful materials for a variety of applications including, but not limited to, general textile applications and specialty applications such as scaffolding materials for tissue engineering. In scaffold design for tissue engineering applications, porosity is a significant parameter to evaluate when gauging the success of a particular scaffold because the cellular environment is crucial to cell viability and migration. Porous biomaterial structures have been formed using techniques such as three-dimensional patterning through stereolithography, phase separation, solvent casting/particulate leaching, gas foaming, and electrospinning. Electrospinning is an attractive technique for forming polymer scaffolds for tissue engineering as it produces a network of fibers of the same order of magnitude as the biological molecules found in the extracellular matrix. Furthermore, although electrospinning is a simple technique to produce fibers with nanometer to micrometer dimensions, there are many variables including solution concentration, applied voltage, needle gauge, and collector distance which influence the morphology of the produced fibers. Accordingly, electrospinning is a technique which allows for significant fine tuning of the final product, by alteration of these various factors. However, until now, it has not been possible to electrospin polymers having a low glass transition temperature (Tg) or low melting point (Tm). Furthermore, electrospinning techniques have previously only been successfully applied to polymers having a high molecular weight.
Poly(propylene fumarate) (PPF) is an unsaturated polyester which has a low melting point (it is liquid at room temperature) and which has been shown to be both biocompatible and biodegradable, having biocompatible degradation products and mechanical properties similar to bone when covalently crosslinked. Because of these properties, PPF has been explored extensively as a scaffold for bone tissue engineering. PPF can be crosslinked thermally or photochemically via the fumarate carbon-carbon double bond, and accordingly, in addition to tissue engineering scaffolds, PPF has been shown to be a promising polymer to use in bone cements where the polymer is applied as a composite forming a putty-like mixture that can be hardened via crosslinking of the fumarate bond. Because PPF is liquid at room temperature, this polymer is particularly attractive for bio-engineering purposes as it can be injected, along with a leachable porogen, into an irregularly shaped defect site and crosslinked in situ. However, due to its low Tg and low Tm, polymers like PPF have not been successfully electrospun.
Previous attempts to form fibers from polymers having low molecular weight and either a low Tg or low Tm have being entirely unsuccessful (See e.g, Song, T.; Zhang, Y. Z.; Zhou, T. J. Fabrication of magnetic composite nanofibers of poly(ε-caprolactone) with FePt nanoparticles by coaxial electrospinning. Journal of Magnetism and Magnetic Materials (2006), 303(2), e286-e289, hereby incorporated by reference). Methods that did succeed, required a high molecular weight polymer or relied on encasing the low Tg polymer material in a high Tg polymer—producing a hybrid material containing both high and low Tg polymers or oligomers. When it was desirable to form a material consisting only of the low Tg or Tm polymer, it was necessary to perform an additional step of removing the high Tg or Tm polymer after fiber formation. (See e.g., McCann Jesse T; Marquez Manuel; Xia Younan Melt coaxial electrospinning: a versatile method for the encapsulation of solid materials and fabrication of phase change nanofibers. Nano letters (2006), 6(12), 2868-72.)
Other methodologies for electrospinning non-woven fiber mats from high molecular weight, low Tg or Tm polymers have relied on chemically modifying the polymer prior to electrospinning (Cashion, M. P.; Brown, R. H.; Mohns, B. R.; Long, T. E., Abstract of Papers, 238th ACS National Meeting, Washington, D.C., United States, Aug. 16-20, 2009, POLY 2009) or were successful only with rubber polymers (Choi, S. S.; Hong, J. P.; Seo, Y. S.; Chung, S. M.; Nah, C., J. Appl. Polym. Sci. 101, 2333 2006).
Accordingly, methodologies which allow for materials including oligomers and some monomers and polymers having characteristics such as low molecular weight, low Tg, and/or low Tm, which have previously made them unsuitable for electrospinning, to be formed into fibers for production of non-woven textiles are greatly desired.
According to an embodiment the present disclosure provides a novel fiber production method for forming continuous sheets of non-woven textiles. According to another embodiment the present disclosure provides novel fibers and/or textiles. In certain embodiments these fibers and/or textiles are formed exclusively from polymers having a low Tg, low Tm, or low molecular weight. In other embodiments these fibers and/or textiles are formed from polymers incorporating other materials in order to produce fibers and textiles having one or more desired properties. In yet another embodiment, the present disclosure provides novel synthesis methods for low molecular weight Poly(propylene fumarate) (PPF) and Poly(propylene fumarate-co-propylene maleate) (PPFcPM).
According to an embodiment the present disclosure provides a novel fiber production method for forming continuous sheets of non-woven textiles. While the presently described method is explained primarily in connection with electrospinning, it will be understood that the presently described method is applicable for use with a wide variety of other textile formation techniques including, but not limited to, meltblowing, melt spinning, dry spinning, wet sinning, gel spinning, single head electrospinning, multihead electrospinning, or flash spinning. Furthermore, the method is applicable for use with all spinning techniques with or without a method to preferentially orient the fibers, including, but not limited to methods that include the use of a mandrel. The method is also applicable for use with all spinning techniques with or without a method to decrease the fiber diameter, including, but limited to methods that incorporate stretching.
According to an embodiment, the fibers and textiles of the present invention are suitable for use in tissue scaffolding applications. For use as a scaffold for tissue engineering, the polymer needs to be easily processed into a highly porous scaffold with a high surface area to volume ratio and an interconnected pore network. Previous research groups have fabricated PPF scaffolds using solvent casting/salt leaching techniques. See, e.g., Porter, B. D.; Oldham, J. B.; He, S. L.; Zobitz, M. E.; Payne, R. G.; An, K. N.; Currier, B. L.; Mikos, A. G.; Yaszemski, M. J., J Biomech Eng 122, 286 2000; Hedberg, E. L.; Kroese-Deutman, H. C.; Shih, C. K.; Crowther, R. S.; Carney, D. H.; Mikos, A. G.; Jansen, J. A., Biomaterials 26, 4616 2005; and Hedberg, E. L.; Shih, C. K.; Lemoine, J. J.; Timmer, M. D.; Liebschner, M. A. K.; Jansen, J. A.; Mikos, A. G., Biomaterials 26, 3215 2005; each of which is hereby incorporated by reference. More recently, high internal phase emulsions (HIPEs) have been used. See e.g., Christenson, E. M.; Soofi, W.; Holm, J. L.; Cameron, N. R.; Mikos, A. G., Biomacromolecules 8, 3806 2007. According to an embodiment, the present disclosure provides a method of fabricating of scaffolds using the established technique of electrospinning. Electrospinning is an attractive technique for forming polymer scaffolds for tissue engineering as it produces a network of fibers of the same order of magnitude as the biological molecules found in the extracellular matrix.
Turning to
Suitable cross-linking agents include, but are not limited to, phenylbis(2,4,6-trimethylbenzoyl)-phosphine oxide (BAPO), acetophenone, 2,2-dimethoxy-2-phenylacetophenone (DMPA), benzophenone, camphorquinone, ferrocene, phenyl azide and any suitable free radical generating photoinitiator. Suitable polymers and oligomers include, but are not limited to, poly(propylene fumarate) (PPF), poly(propylene fumarate-co-propylene maleate) (PPFcPM), poly(butylene fumarate) (PBF), poly(butylene fumarate-co-butylene maleate) (PBFcBM), polymers or oligomers containing terminal or pendant acrylate groups, polymers or polymers or oligomers containing terminal or pendant methacrylate groups, or other phenyl azide modified polymers. It is noted that the method described herein is particularly well suited for polymers and oligomers which were previously incapable or being electrospun including those having low Tgs, Tms, or molecular weights. According to various embodiments and for the purposes of the present disclosure, a low Tg is defined as a glass transition temperature below that of ambient room temperature, a low Tm is defined as a melting point below that of ambient room temperature, and a low molecular weight is defined as a molecular weight below 10,000.
In some cases the molecular weight may be lower than 10,000 such as 6000, 2000, 1000, 500 or lower. However, polymers having higher Tgs, Tms or molecular weights are also suitable for use with the presently described methodologies. Furthermore it is noted that unlike previous methodologies wherein low Tg polymers were formed into fibers by encasing them in high Tg polymers, the methods of the present invention can be utilized to make fibers and, indeed, textiles formed exclusively from low Tg, Tm, or low molecular weight polymers and/or monomers.
Alternatively, rather than incorporating the cross-linking agent into the solution, the polymer (or oligomer) to be electrospun may be decorated with a photoactive moiety that enables cross-linking. Those of skill in the art will be familiar with polymer modification techniques that may be utilized to decorate polymers and oligomers. For example, polymers containing functional groups such as aldehyde, alkene, alkyne, azides, amine, carboxylic acids, cyanates, cyclic ethers, epoxy, esters, halide, hydroxyl, isocyanates, ketones, nitriles, and thiols can all be functionalized with photoactive groups. Polymers can be carbon based, ether based, ester based, urea based, or silicone based materials. Polymers can be functionalized with one or more, preferably more photoactive groups that form direct carbon-carbon bonds such as acetylene, acrylate, cinnamate, fumarate, maleate, methacrylate, or olefinic groups with or without the addition of a photogenerated radical initiator. Alternatively, polymers or oligomers can be modified with one or more, preferably more groups that can be polymerized or cross-linked with the use of a photogenerated catalyst including both photoacid and photobase generators. Functional groups which can be photopolymerized using acid or base catalysis include groups such as cyclic ethers, cyclic ethers, and epoxy and all negative tone photoresists. Alternatively, polymers or oligomers can be modified with one or more, preferably more groups that undergo a photo-activated click reaction such as the thiol-ene, thiol-yne, photo Huisgen, or photo induced diels-alder reaction.
Furthermore, rather than, or in addition to, modifying the polymers (or oligomers) with a photoactive group, the polymers may be modified with or otherwise incorporate other desirable materials in order to produce textiles having desired physical or chemical properties or characteristics. These polymer composites may include fillers such as single-walled carbon nanotubes, multi-walled carbon nanotubes, metal based micro- or nano-particles, carbon based micro- or nano-particles, ceramic micro- or nano-particles, semiconductor micro- or nano-particles, cells, and pharmaceutical agents.
As stated above, suitable polymers and oligomers include, but are not limited to, poly(propylene fumaratefumarate) (PPF), poly(propylene fumarate-co-propylene maleate) (PPFcPM), poly(butylene fumarate) (PBF), poly(butylene fumarate-co-butylene maleate) (PBFcBM). According to an embodiment, the present disclosure provides novel methods for synthesizing PPF and PPFcPM. An exemplary synthesis scheme for the production of PPF and PPFcPM is shown in
All patents and publications referenced or mentioned herein are indicative of the levels of skill of those skilled in the art to which the invention pertains, and each such referenced patent or publication is hereby incorporated by reference to the same extent as if it had been incorporated by reference in its entirety individually or set forth herein in its entirety. Applicants reserve the right to physically incorporate into this specification any and all materials and information from any such cited patents or publications. The specific methods and compositions described herein are representative of preferred embodiments and are exemplary and not intended as limitations on the scope of the invention. Other objects, aspects, and embodiments will occur to those skilled in the art upon consideration of this specification, and are encompassed within the spirit of the invention as defined by the scope of the claims. It will be readily apparent to one skilled in the art that varying substitutions and modifications may be made to the invention disclosed herein without departing from the scope and spirit of the invention. The invention illustratively described herein suitably may be practiced in the absence of any element or elements, or limitation or limitations, which is not specifically disclosed herein as essential. The methods and processes illustratively described herein suitably may be practiced in differing orders of steps, and that they are not necessarily restricted to the orders of steps indicated herein or in the claims. As used herein and in the appended claims, the singular forms “a,” “an,” and “the” include plural reference unless the context clearly dictates otherwise. Thus, for example, a reference to “a host cell” includes a plurality (for example, a culture or population) of such host cells, and so forth.
Under no circumstances may the patent be interpreted to be limited to the specific examples or embodiments or methods specifically disclosed herein. Under no circumstances may the patent be interpreted to be limited by any statement made by any Examiner or any other official or employee of the Patent and Trademark Office unless such statement is specifically and without qualification or reservation expressly adopted in a responsive writing by Applicants.
The terms and expressions that have been employed are used as terms of description and not of limitation, and there is no intent in the use of such terms and expressions to exclude any equivalent of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the invention as claimed. Thus, it will be understood that although the present invention has been specifically disclosed by preferred embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention as defined by the appended claims.
The invention has been described broadly and generically herein. Each of the narrower species and subgeneric groupings falling within the generic disclosure also form part of the invention. This includes the generic description of the invention with a proviso or negative limitation removing any subject matter from the genus, regardless of whether or not the excised material is specifically recited herein. In addition, where features or aspects of the invention are described in terms of Markush groups, those skilled in the art will recognize that the invention is also thereby described in terms of any individual member or subgroup of members of the Markush group.
General Procedure. All reactions were carried out under a dry atmosphere unless noted. 1H nuclear magnetic resonance (NMR) was carried out on a 400 MHz Bruker DRX-AVANCE. Proton chemical shifts (δ) are reported as shifts from the internal standard tetramethylsilane (TMS). Infrared Spectroscopy (IR) was carred out on a Nicolet 6700 FTIR. Gel Permeation Chromatography (GPC) molecular weight determinations were performed by GPC using a Polymer Labs 220 PL-GPC equipped with a UV-Vis detector. Two columns (PLgel 5 μm MiniMIC-C, 250×4.6 mm) and a guard column (PLgel 5 μm MiniMIX-C, 50×4.6 mm) were used in series with a flow rate of 0.4 mL/min and a run pressure of 6.0 MPa. Chloroform was used as the eluent (0.4 mL/min), and measurements were performed at 35° C. Calibration was performed using polystyrene standards with a narrow molecular weight distribution (Fluka ReadyCal 400-2,000,000). Scanning electron microscopy (SEM) was carried out using a Zeiss Supera 55VP and a FEI DB235. Differential Scanning calorimeter (DSC) measurements, used to determine Tg, were performed using a TA Instruments DSC100. Viscosity determination was done using a Brookfield DV-E Viscometer, reported in cP (60 rpm, spindle #14). p-Toluensulfonic acid (TsOH), monohydrate 99%, extra pure was purchased from Acros. Ethyl acetate, HPLC grade, anhydrous magnesium sulfate (MgSO4), anhydrous and sulfuric acid, certified ACS plus were purchased from Fisher. 1,2-Propanediol, 99% (PD), maleic anhydride (MA), briquettes 99%, Zinc chloride, anhydrous powder ≧99.995% trace metals, Iron (III) Chloride, reagent grade 97%, phenylbis(2,4,6-trimethylbenzoyl)-phosphine oxide, 97% and benzyl, 98% were all purchased from Aldrich. All chemicals were used as received from suppliers.
General Method A Poly(Propylene Fumarate-co-Propylene Maleate) Synthesis. MA, PD, toluene and catalyst were added to a round bottom flask equipped with stir bar and Dean-Stark (DS) trap for azeotropic distillation. The reaction was allowed to proceed at a maximum temperature of 110° C., until no more distillate (water) was collected. The reaction mixture was cooled to RT, upon cooling toluene was removed in vacuo, the crude polymer was then dissolved in ethyl acetate (EtOAc) and washed with distilled water (3×). The organic layer was then dried over anhydrous MgSO4 and solvent again removed in vacuo.
General Method B Poly(Propylene Fumarate-co-Propylene Maleate) Synthesis. MA, PD and toluene were added to a round bottom flask. The reaction mixture was heated to 50° C. and stirred overnight. The reaction mixture was allowed to cool to RT and the toluene was removed in vacuo. The reaction flask was then equipped with a DS trap and condenser to collect water through azeotropic distillation during the second reaction. Next, a protic acid catalyst was added to the product of the first reaction, and the mixture heated to a maximum temperature of 110° C., until the appropriate volume of water was collected. The reaction mixture was allowed to cool to RT, the solvent was removed in vacuo, and the crude polymer was dissolved in ethyl acetate and washed with distilled water (3×). Finally, the organic layer was dried over anhydrous MgSO4 and solvent removed in vacuo.
PPF Synthesis (1). MA (10.0 g, 102 mmol), PD (7.8 g, 102 mmol), and tosic acid (0.02 g, 0.1 1 mmol) was added to a 100 mL round bottom flask equipped with a stir bar and distillation head. The reaction mixture was heated to 250° C. with stifling. After 3 hrs, the reaction was allowed to cool to RT. The resulting viscous crude polymer was dissolved in ethyl acetate (50 mL) and washed with distilled water (50 mL, 3×). The organic layer was dried over anhydrous MgSO4, filtered and solvent removed in vacuo to yield a slightly yellow viscous polymer. IR (neat) 2984.1, 1714.7, 1645.4, 1454.7, 1379.0, 1290.2, 1255.5, 1153.4, 1116.2, 1075.9, 1022.5, 979.1, 837.3, 753.5, 666.4 cm-1. 1H NMR (400 MHz, CDCl3) δ 6.88-6.78 (m, —CH═CH—), 5.25-5.2 (m, —CH(CH3)), 4.68-2.8 (m, —OCO—CH2—), 1.43-1.15 (m, (CH3)CH2). GPC (1 mg/mL, CHCl3) Mw 949 Mn 473. Tg (° C.) 15.24.
Method A PPFcPM Synthesis (2). MA (10.0 g, 102 mmol), PD (7.8 g, 102 mmol) and toluene (30-50 mL) and the appropriate catalyst, TsOH (0.2 g, 1.0 mmol), H2SO4 (1 drop, 18N), ZnCl2 (0.14 g, 1.0 mmol) or FeCl3 (0.17 g, 1 mmol), were added to a 100 mL round bottom flask equipped with stir bar along with DS trap and condenser. The reaction mixture was allowed to progress overnight. The reaction was ended and brought to RT, upon cooling toluene was removed in vacuo. The crude polymer was then dissolved in ethyl acetate (50 mL) and washed with water (50 mL, 3×), drying the organic phase over anhydrous MgSO4 and removing the solvent to yield a viscously clear polymer.
PPFcPM synthesized with TsOH: IR (neat) 3490.0, 3058.6, 2983.4, 1711.9, 1643.6, 1455.3, 1384.2, 1252.6, 1077.7, 983.6, 828.7, 777.3 cm-1. 1H NMR (400 MHz, CDCl3) δ 7.17-7.14 (m, Ar), 7.09-7.03 (m, Ar), 6.83-6.76 (m, trans —CH═CH), 6.27-6.13 (m, cis —CH═CH—) 5.19-5.17 (bs, —CH(CH3)), 4.34-3.61 (m, —OCO—CH2—), 2.26 (s, CH3—Ar), 1.25-1.03 (m, (CH3)CH2—). GPC (1 mg/mL, CHCl3) Mw 995 Mn 728. Tg (° C.) −40.38.
PPFcPM synthesized with ZnCl2: IR (neat) 3516.3, 3079.6, 2984.3, 2943.7, 2883.4, 1711.1, 1644.0, 1452.5, 1381.1, 1356.2, 1289.2, 1251.9, 1224.0, 1149.6, 1116.0, 1075.9, 1019.6, 978.3, 835.7, 773.5, 668.1 cm-1. 1H NMR (400 MHz, CDCl3) δ 7.22-7.20 (m, Ar), 7.14-7.10 (m, Ar), 6.90-6.76 (m, trans —CH═CH), 6.23-6.20 (m, cis —CH═CH—) 5.27-5.07 (m, —CH(CH3)), 4.40-4.02 (m, —OCO—CH2—), 2.32 (s, CH3—Ar), 1.51-1.23 (m, (CH3)CH2-). GPC (1 mg/mL, CHCl3) Mw 1297 Mn 824. Tg (° C.) −18.66.
PPFcPM synthesized with FeCl3: IR (neat) 3445.0, 3235.5, 3081.1, 2985.9, 2661.0, 2362.5, 1716.2, 1751.0, 1700.4, 1646.7, 1455.9, 1386.3, 1355.4, 1324.4, 1279.4, 1190.8, 1121.8, 1080.2, 990.2, 838.6, 775.3 cm-1. 1H NMR (400 MHz, CDCl3) δ 6.93-6.83 (m, trans —CH═CH), 6.33-6.23 (m, cis —CH═CH—) 5.27-5.10 (m, —CH(CH3)), 4.40-4.10 (m, —OCO—CH2—), 1.44-1.23 (m, (CH3)CH2—). GPC (1 mg/mL, CHCl3) Mw 1871 Mn 1043. Tg (° C.) −37.58.
PPFcPM synthesized with H2SO4: IR (neat) 3526.2, 3079.3, 2984.1, 1716.1, 1645.5, 1558.5, 1541.9, 1508.1, 1456.2, 1379.8, 1253.1, 1217.4, 1150.1, 1113.8, 1074.7, 977.1, 833.2, 773.2 cm-1. 1H NMR (400 MHz, CDCl3) δ 7.23-7.20 (m, Ar), 7.15-7.10 (m, Ar), 6.88-6.82 (m, trans —CH═CH), 6.34-6.24 (m, cis —CH═CH—) 5.24 (bs, —CH(CH3)), 4.77-4.00 (m, —OCO—CH2—), 2.32 (s, CH3—Ar), 1.44-1.21 (m, (CH3)CH2—). GPC (1 mg/mL, CHCl3) Mw 672 Mn 330. Tg (° C.) −12.86.
Method B PPFcPM Synthesis (2). MA (10.0 g, 102 mmol), PD (7.8 g, 102 mmol) and toluene (15 mL) were added to a 100 mL round bottom flask equipped with a stir bar. Under a nitrogen blanket, the reaction heated to 50° C. with stifling was allowed to run overnight. The next day, the reaction mixture was allowed to cool to RT and the solvent removed in vacuo. The reaction flask was then equipped with a DS trap and condenser. To the product of the first reaction, toluene and either tosic acid (0.2 g, 1 mmol) or sulfuric acid (1 drop, 18 N) was added. The reaction was allowed to run until 1.6 mL of water was collected via the DS trap. The reaction was allowed to come to RT and the solvent was removed in vacuo. The crude polymer was then dissolved in ethyl acetate (50 mL) and washed with water (50 mL, 3×). The organic layer was dried over MgSO4 with filtration and the solvent was removed in vacuo to yield a slightly yellow viscous polymer.
PPFcPM synthesized with TsOH: IR (neat) 2985.9, 1721.6, 1691.3, 1644.4, 1454.6, 1381.1, 1289.9, 1252.0, 1215.8, 1152.4, 1116.1, 1075.4, 979.0, 838.2, 774.3, 736.5, 669.0 cm-1. 1H NMR (400 MHz, CDCl3) δ 6.86-6.83 (m, trans —CH═CH—), 6.29-6.23 (m, cis —CH═CH—), 5.24 (bs, —CH(CH3)), 4.78-3.44 (m, —OCO—CH2), 1.32-1.17 (m, (CH3)CH2—). GPC (1 mg/mL, CHCl3) Mw 11388 Mn 2347. Tg (° C.) −13.78.
PPFcPM synthesized with H2SO4: IR (neat) 2985.7, 1717.7, 1643.6, 1454.7, 1382.5, 1253.8, 1151.8, 1116.5, 1075.3, 978.7, 889.8, 838.1, 7775.0, 734.6, 694.8 cm-1. 1H NMR (400 MHz, CDCl3) δ 7.24-7.21 (m, Ar), 7.16-7.11 (m, Ar), 6.83 (s, trans —CH═CH—), 6.25 (s, cis —CH═CH—) 5.26 (bs, —CH(CH3)), 4.78-2.75 (m, —OCO—CH2—), 2.33 (s, CH3—Ar), 1.33-1.17 (m, (CH3)CH2-). GPC (1 mg/mL, CHCl3) Mw 5520 Mn 1739. Tg (° C.) −13.78
General Procedure for Electrospinning. All polymer solutions were delivered at a constant rate via a syringe pump (KD scientific, model 100s); through a syringe fitted with stainless steel blunt tip needle (Small Parts, Inc.). The needle was charged through a high voltage supply (Glassman High Voltage, Inc. Series EL), and the resulting polymer fibers were collected on a grounded target (6×6 in2 Cu plate fitted with Al foil). A UV source (UVP, Blak-Ray longwave ultraviolet lamp, model B100AP, λ=365 nm) was used to crosslink polymer solution in-situ (
Electrospinning PPF and PPFcPM. A 2 mL plastic syringe (inner diameter (ID)=4.64 mm) equipped with a 20 gauge (g)×1.5 in. stainless steel blunt tip needle was used to deliver solutions of polymer dissolved in chloroform (40, 50 and 60 wt %) at a volumetric flow rate of 0.2 mL/hr and a voltage difference of 1 kV/cm from needle tip to collection plate.
Crosslinking While Electrospinning PPF and PPFcPM. A 2 ml plastic syringe (ID=4.64 mm) equipped with a 20 g×1.5 in stainless steel blunt tip needle was used to deliver a 50 wt % polymer solution with a 3 wt % initiator (benzil or phenylbis(2,4,6-trimethylbenzoyl)-phosphine oxide (BAPO)) in chloroform. The polymer solution was spun at a constant rate of 0.1 mL/hr and a voltage of 1 kV/cm, from needle tip to collection plate. While the polymer was being collected on the target it was being crosslinked via the UV source.
Crosslinked PPFcPM: IR (neat) 2957.6, 1719.1, 1643.6, 1453.2, 1382.9, 1254.2, 1209.4, 1150.8, 1114.3, 1073.3, 978.7, 813.9, 752.7, 667.5 cm−1.
Results and Discussion
Poly(propylene-fumarate) (PPF) and poly(propylene fumarate)-co-(propylene maleate) (PPFcPM) were synthesized via step growth polycondensation reactions (
The first method (Method A) used to synthesize the copolymer involved a protic acid or Lewis acid catalyzed polymerization reaction carried out at 85° C. to 110° C. to azeotropically remove water. The second method (Method B) involved an initial ring opening reaction carried out at 50° C. without the use of a catalyst followed by an acid catalyzed condensation reaction in combination with azeotropic removal of water.
The ratio of fumarate to maleate in the polymer was influenced by both temperature and catalyst (
PPFcPM synthesized using sulfuric acid as the catalyst resulted in toluene inclusion due to Friedel-Craft alkylation. See e.g, Ipatieff, V. N.; Corson, B. B.; Pines, H., J. Am. Chem. Soc. 58, 919 1936, which is hereby incorporated by reference. The influence of temperature and catalyst was also observed in all of the one step azeotropic distillation scenarios, thus providing a system which has the ability to be adjusted.
The molecular weights of all polymers produced were determined through gel permeation chromatography using narrow weight distribution polystyrene as the standards. PPF synthesized according to Method A had an average molecular weight (Mn) of 720, with poly dispersity (PDI) of 2.0. The molecular weight did not increase with longer reaction times (data not shown). The low molecular weight is consistent with the initial production of PPFcPM oligomers which thermally isomerizes to the more stable fumarate form. Presumably the high temperature results in both isomerization and side reactions that limit the polymer molecular weight by changing the step growth stoichiometry. PPF synthesized in this fashion is about 70% lower in molecular weight than other reported synthesis (see e.g., Fisher, J. P.; Holland, T. A.; Dean, D.; Engel, P. S.; Mikos, A. G., J. Biomater. Sci., Polym. Ed. 12, 673 2001, hereby incorporated by reference), however PPF is isolated via a two step synthesis in the previously reported synthesis. PPFcPM synthesized through one step synthesis (Method A) also resulted in polymers with low molecular weights (
To form a network of PPFcPM copolymer fibers, the copolymer was spun using standard electrospinning techniques. Three different solution concentrations ranging from 40 to 60% (w/w) dissolved in chloroform were used to determine the solution concentration that would allow for the production of continuous fibers at 1 kV/cm (
In order to produce a fibrous 3D network that did not self-calendar the copolymer was crosslinked using in-situ photopolymerization during the electrospinning process. Crosslinking the polymer before electrospinning was not possible as the polymer would no longer be soluble.
Either benzyl or BAPO was incorporated at 3% (w/w) into a PPFcPM solution (40-60% (w/w)) in chloroform, yielding a solution viscosity of 1863 cp (Brookfield DV-E) at RT. Both solutions were electrospun using the aforementioned parameters and set up. The nano- and microfibers fabricated from a polymer solution containing benzil were exposed to UV light (λ=365 nm) as they were spun and deposited onto the aluminium foil coated copper plate held at ground potential. After deposition the polymer was exposed to UV radiation for an additional 15 min. Fibers produced in this way did not exist as individual fibers but rather as a self calendared layer (
In order to determine the cause of the pillar formation, a temperature mapping of the aluminum foil coated plate was performed by splitting the aluminum foil into a 3×3 array of 2″ squares to form a total of nine regions. Using an IR thermometer, the temperature was recorded in each of the regions to determine if the UV lamp was locally heating the aluminum surface, potentially leading to pillar formation. No local heating of the surface was observed over a typical period of electrospun fiber deposition. Further examination of the electrospinning apparatus revealed that the UV radiation was being reflected off the aluminum foil exposing the PPFcPM/BAPO filled syringe, promoting photo-crosslinking of the polymer solution altering the solution viscosity. However, when the syringe was shielded from the reflected UV radiation the PPFcPM/BAPO was spun successfully and produced a non-calendared mat, free of pillar formation (
| Number | Name | Date | Kind |
|---|---|---|---|
| 4421707 | Kourtz et al. | Dec 1983 | A |
| 6753358 | Fisher et al. | Jun 2004 | B2 |
| 20100064647 | Brands et al. | Mar 2010 | A1 |
| Number | Date | Country |
|---|---|---|
| WO 2006056740 | Jun 2006 | WO |
| Entry |
|---|
| Bone-tissue-engineering material poly(propylene fumarate): correlation between molecular weight, chain dimensions, and physical properties. Biomacromolecules. Wang S. Lu L. Yaszemski M.J. 2006;7:1976. |
| Synthesis of poly(propylene fumarate). Nat Protoc. Kasper FK, Tanahashi K, Fisher JP, Mikos AG. 2009;4:518-525. |
| Choi, S. S., Hong, J. P., Seo, Y. S., Chung, S. M., and Nah, C., Fabrication and Characterization of Electrospun Polybutadiene Fibers Crosslinked by UV Irradiation, Oct. 7, 2005, Journal of Applied Polymer Science, vol. 101, pp. 2333-2337. |
| Gupta et al., In Situ Photo-Cross-Linking of Cinnamate Functionalized Poly(methyl methacrylate-co-2-hydroxyethyl acrylate) Fibers during Electrospinning, Jun. 11, 2004, vol. 37, pp. 9211-9218. |
| Cashion, M. P.; Brown, R. H.; Mohns, B. R.; Long, T. E., Abstract of Papers, 238th ACS National Meeting, Washington, DC, United States, Aug. 16-20, 2009, POLY 2009. |
| Choi, S. S.; Hong, J. P.; Seo, Y. S.; Chung, S. M.; Nah, C., J. Appl. Polym. Sci. 101, 2333 2006. |
| Choi, et al., “Effect of Photopolymerization on the Rate of Photocrosslink in Chalcone-based Oligomeric Compounds,” Bull. Korean Chem. Soc. 2001, vol. 22, No. 11 1207. |
| Number | Date | Country | |
|---|---|---|---|
| 61280875 | Nov 2009 | US |
