Claims
- 1. A vector for transforming a plant comprising a nucleic acid encoding a multifunctional fatty acid synthase.
- 2. The vector of claim 1, further comprising a second nucleic acid encoding a phosphopantetheine:protein transferase enzyme.
- 3. The vector of claim 1, wherein the multifunctional fatty acid synthase nucleic acid is isolated from Brevibacterium ammoniagenes (ATCC 5871), Schizosaccharomyces pombe, Saccharomyces cerevisiae, Candida albicans, Mycobacterium tuberculosis, Mycobacterium leprae, Mycobacterium bovis, Caenorhabditis elegans, rat, or chicken.
- 4. The vector of claim 1 or 2, wherein the nucleic acid encodes a multifunctional fatty acid synthase having an amino acid sequence selected from the group consisting of SEQ ID NOS: 2, 15, 17, 18, 19, 20, 22-25, 27, 29, and 31.
- 5. The vector of claim 1 or 2, wherein the nucleic acid encoding a multifunctional fatty acid synthase comprises the DNA selected from the group consisting of SEQ ID NOS: 1, 16, 21, 26, 28, and 30, and complements thereof, and DNAs having at least about 70% sequence identity thereof.
- 6. The vector of claim 5, wherein the DNA is SEQ ID NO: 1.
- 7. The vector of claim 2, wherein the second nucleic acid encoding a phosphopantetheine:protein transferase enzyme encodes an amino acid sequence selected from the group consisting of SEQ ID NOS: 4, 33, 35, 37, 39, 41, and 43.
- 8. The vector of claim 2, wherein the second nucleic acid comprises DNA selected from the group consisting of SEQ ID NOS: 3, 32, 34, 36, 38, 40, and 42, and complements thereof, and DNAs having at least about 80% sequence identity thereof.
- 9. The vector of claim 2, wherein the vector further comprises a nucleic acid encoding a promoter that is operably linked to the nucleic acid encoding a multifunctional fatty acid synthase or to the nucleic acid encoding a phosphopantetheine:protein transferase enzyme, wherein the promoter is functional in a plant.
- 10. A method for expressing a heterologous multifunctional fatty acid synthase in a plant comprising transforming a plant with the vector of claim 4.
- 11. A plant comprising a heterologous nucleic acid encoding a multifunctional fatty acid synthase.
- 12. The plant of claim 11, further comprising a second heterologous nucleic acid encoding a phosphopantetheine:protein transferase enzyme.
- 13. The plant of claim 11 or 12, wherein the multifunctional fatty acid synthase nucleic acid is isolated from Brevibacterium ammoniagenes (ATCC 5871), Schizosaccharomyces pombe, Saccharomyces cerevisiae, Candida albicans, Mycobacterium tuberculosis, Mycobacterium leprae, Mycobacterium bovis, Caenorhabditis elegans, rat, or chicken.
- 14. The plant of claim 11 or 12, wherein the nucleic acid encodes a multifunctional fatty acid synthase comprising an amino acid sequence selected from the group consisting of SEQ ID NOS: 2, 15, 17, 18, 19, 20, 22-25, 27, 29, and 31.
- 15. The plant of claim 11 or 12 wherein the nucleic acid encoding a multifunctional fatty acid synthase comprises the DNA selected from the group consisting of SEQ ID NOS: 1, 16, 21, 26, 28, and 30, and complements thereof, and DNAs having at least about 80% sequence identity thereof.
- 16. The plant of claim 12, wherein the nucleic acid encoding a phosphopantetheine:protein transferase enzyme encodes an amino acid sequence comprising a protein selected from the group consisting of SEQ ID NOS: 4, 33, 35, 37, 39, 41, and 43.
- 17. The plant of claim 12, wherein the second nucleic acid comprises DNA selected from the group consisting of SEQ ID NOS: 3, 32, 34, 36, 38, 40, and 42, and compliments thereof, and DNAs having at least about 80% sequence identity thereof.
- 18. The plant of claim 12, that further comprises a promoter that is operably linked to the nucleic acid encoding a multifunctional fatty acid synthase or the second nucleic acid encoding a phosphopantetheine:protein transferase enzyme, wherein the promoter is functional in a plant cell.
- 19. The plant of claim 18, wherein the promoter provides expression substantially within plant seeds of the multifunctional fatty acid synthase or the phosphopantetheine:protein transferase enzyme.
- 20. A method of increasing oil in a tissue of a plant, comprising expressing a gene encoding a mfFAS, wherein the mfFAS is from a species of the group consisting of Brevibacterium ammoniagenes, Schizosaccharomyces pombe, Saccharomyces cerevesiae, Candida albicans, Mycobacterium tuberculosis, Caenorhabditis elegans, Rattus norvegicus, Gallus gallus, Lipomyces starkeyi, Rhodosporidium toruloides, and Mycobacterium bovis, and wherein the plant is not Brasssica, and wherein the plant is not soybean when the mfFAS is from Brevibacterium ammoniagenes.
- 21. A method of increasing oil in a corn plant, comprising expressing a gene encoding a mfFAS, wherein the mfFAS is from Lipomyces starkeyi.
Parent Case Info
[0001] The patent application claims the benefit of U.S. Provisional Patent Application No. 60/435,197, filed Dec. 19, 2002, which is incorporated by reference herein.
Provisional Applications (1)
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Number |
Date |
Country |
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60435197 |
Dec 2002 |
US |