EMBRYONIC CEREBROSPINAL FLUID (e-CSF), PROTEINS FROM e-CSF, AND RELATED METHODS AND COMPOSITIONS

BACKGROUND OF THE INVENTION

During the process of neurulation the neural groove forms and the neural folds fuse to form the neural tube. Once the neural tube is fused, the fluid within the lumen is considered cerebrospinal fluid, whereas before fusion is complete the neuroepithelium lining the inside of the neural tube is still in contact with amniotic fluid. During the early stages of neural tube growth and development, groups of specialized neuroepithelial cells lining the neural tube are believed to secrete fluid into the neural tube space in order to support growth and development of the embryo. As the neural tube continues to elongate and develop, specific highly vascularized epithelial cell types begin to invaginate at specific locations within the neural tube to form the specialized choroid plexus.

The choroid plexus is a highly vascularized epithelial cell structure that during development may be involved in the specific intracellular transfer of proteins into the CSF from the blood (Saunders et al., Cell Mol Neurobiol, 2000. 20:29-40). The choroid plexus develops in the lateral ventricles and in the third and fourth ventricles of the brain. In rats, the choroid plexus can be first identified as early as embryonic day 13 (E13) as a midline structure and by E15 it represents paired structures protruding into the lateral ventricles. In the human embryo the choroid plexus begins to develop in the lateral and fourth ventricle at Carnegie Stage (CS) 18, approximately 44 days post-ovulation. The first appearance of cerebral cortical neurons in the human embryo occurs at CS 21, shortly following the appearance of the choroid plexus and the production of CSF, and a similar temporal sequence is seen in mice and rats.

In adults, CSF has many functions, including an intermediary between blood and brain for the transport of nutrients and growth factors and as a fluid buffer for the brain to protect both the brain and the large vessels that supply blood to the brain (Chodobski et al., Microsc Res Tech, 2001. 52:65-82; Emerich et al., Bioessays, 2005. 27:262-74). It may also be involved in elimination of toxins and other metabolic byproducts (Emerich et al., Bioessays, 2005. 27:262-74; Miyan et al., Can J Physiol Pharmacol, 2003. 81:317-28). A mathematical analysis taking into account the pulsatile nature of CSF flow suggested that the CSF pulsations buffer the capillary bed from the effects of arterial pulsations that might otherwise prevent linear blood flow due to the mechanics of the brain being enclosed in the skull (Miyan et al., Can J Physiol Pharmacol, 2003. 81:317-28). CSF contains nerve growth factor (NGF), transforming growth factor alpha (TGF-alpha); levels of these proteins are altered in neurological and developmental disorders (Miyan et al., Can J Physiol Pharmacol, 2003. 81:317-28; Kasaian et al., Biofactors, 1989. 2:99-104; Massaro et al., Ital J Neurol Sci, 1994. 15:105-8; Patterson et al., Brain Res, 1993. 605:43-9; Van Setten et al., Int J Dev Neurosci, 1999. 17:131-4), but potential functions of these factors has not been demonstrated. Recently it was shown that the ciliary action of CSF in the lateral ventricle of adult rats creates a gradient of SLIT2 protein, a chemorepulsive factor for neuronal olfactory bulb migration, within the CSF (Sawamoto et al., Science, 2006. 311:629-32), suggesting that CSF factors might have instructive roles for developing neurons or neural progenitors.

Although the role of the CSF during embryogenesis is just starting to be studied, an important role has been suggested in brain development (Miyan et al., Can J Physiol Pharmacol, 2003. 81:317-28; Gato et al., Anat Rec A Discov Mol Cell Evol Biol, 2005. 284:475-84; Martin et al., Dev Biol, 2006. 297:402-16; Mashayekhi et al., Brain, 2002. 125:1859-74; Miyan et al., Cerebrospinal Fluid Res, 2006. 3:2; Owen-Lynch et al., Brain, 2003. 126:623-31). Miyan et al. have shown that rat cortical cells are viable and proliferate in e-CSF (Miyan et al., Cerebrospinal Fluid Res, 2006. 3:2). Other studies have tested discrete signaling factors that may regulate neurogenesis. Gato et al. and Martin et al. have studied the role of chick e-CSF in regulating survival, proliferation, and neurogenesis of neuroepithelial cells, and have identified FGF-2 in the chick CSF as a vital trophic factor (Gato et al., Anat Rec A Discov Mol Cell Evol Biol, 2005. 284:475-84; Martin et al., Dev Biol, 2006. 297:402-16). Intriguingly, in mutant animals, CSF factors that may inhibit proliferation have been suggested. In studies of the hydrocephalic Texas (H-Tx) rat, cell proliferation in the ventricular zone decreases, and although cell migration still occurs, there is a decrease in the number of migrating cells (Mashayekhi et al., Brain, 2002. 125:1859-74; Miyan et al., Cerebrospinal Fluid Res, 2006. 3:2). In addition, CSF from the lateral ventricles of affected H-Tx fetuses can completely inhibit in vitro proliferation of neuronal progenitors isolated from a normal fetus at 10% CSF addition to the media, suggesting that factors intrinsic to the CSF of the H-Tx fetuses are present that inhibit proliferation.

Prior to the present invention, the identification of such CSF factors with a developmental role has been impeded, as the components of the CSF were previously not known. While a first glimpse of the protein composition of e-CSF has been provided, a (Parada et al., Proteomics, 2006. 6:312-20; Parada et al., J Proteome Res, 2005. 4:2420-8), a complete analysis of the contents of e-CSF would be allow for identification of proteins important for neural developmental and differentiation.

SUMMARY OF THE INVENTION

We have developed methods for isolating embryonic cerebrospinal fluid (e-CSF) and have identified the proteins found in rat and human e-CSF. On this basis, the invention features a method of isolating embryonic cerebrospinal fluid (e-CSF). As e-CSF is capable of supporting the culture of developing neural cells, the invention also features methods of culturing cells in the presence of various components of the e-CSF, as well as compositions including cells and e-CSF component(s).

In a first aspect, the invention features a composition including at least one (e.g., at least 2, 3, 4, 5, 6, 8, 10, 15, 25, 50, 75, or 100) component(s) of e-CSF (e.g., rat, mouse, or human). The composition may include the component or components at an enhanced level relative to the level in e-CSF and the composition is capable of supporting proliferation, maintenance, or differentiation of a cultured cell (e.g., a stem cell or progenitor cell such as neural cell). The component may be a polypeptide, or a functional fragment thereof (e.g., a soluble fragment). The polypeptide may be isolated, purified, or produced recombinantly. The component may be present at a level sufficient to enhance cell proliferation, maintenance, or differentiation, as compared to in the absence of the component. The component may be one that is not found in adult CSF.

The invention also features a cell culture composition including a cell (e.g., any described herein) and a composition of the first aspect.

The invention also features a kit including (a) a composition including at least one component of e-CSF, wherein the component is present at an enhanced level relative to naturally occurring e-CSF (e.g., the compositions described above); and (b) instructions for using (a) for cell culture.

In another aspect, the invention features a method of culturing a cell (e.g., a stem cell or a progenitor cell, such as a neural cell), including incubating the cell in culture media containing at least one isolated component of rat or human e-CSF (e.g., any of the compositions of the first aspect of the invention). The component may be a polypeptide, or a functional fragment thereof (e.g., a soluble fragment). The polypeptide may be isolated, purified, or produced recombinantly. In certain embodiments, the component is one which is not found in adult CSF.

In any of the above aspects, the e-CSF component may be one described in Tables 1-4.

In another aspect, the invention features a method of isolating embryonic cerebrospinal fluid (e-CSF) including (a) providing an embryo; (b) inserting a capillary needle into a ventricle of the central nervous system of the embryo such that the tip of the needle contacts CSF; and (c) extracting CSF from the embryo through the needle (e.g., a microcapillary pipette or syringe), thereby isolating e-CSF. The method may further include (d) removing intact contaminating cells (e.g., by filtration or centrifugation). Step (c) may be performed such that the needle tip does not contact the neuroepithelium during the extraction. The e-CSF may be removed from a lateral ventricle or from the third or fourth ventricle of the embryo, or a combination thereof. The method may further include storing the e-CSF at less than about 0-20° C. to about −80, −90, −100, −150° C.

By “isolated” is meant, with respect to a naturally occurring compound (e.g., a polypeptide), that the compound is at least partially free from the components (e.g., other polypeptides, nucleic acids, cell membranes) with which it naturally is found.

By “purified” is meant, with respect to a compound (e.g., a polypeptide), that the compound makes up at least 20% (e.g., at least 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, or 99%) of the composition with which is it found.

By “enhanced level” of a component is meant that the component is present either a higher concentration (e.g., at least 10%, 25%, 50%, 100%, 250%, 500%, or 1000% greater) or at higher purity level (e.g., with 5%, 10%, 25%, 50%, or 75% less by mass other components, not including solvents or buffers) relative to the concentration or purity of the component in a control composition (e.g., a naturally occurring composition).

By “stem cell” is meant a self-renewing cell that is capable of differentiation into multiple mature cell types (e.g., a neuron, glial cell, or astrocyte).

By “progenitor cell” (e.g., neural progenitor cells) is meant a cell that is capable of forming at least one cell type has at least some capacity for self-renewal.

Other features and advantages of the invention will be apparent from the following Detailed Description, the drawings, and the claims.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A-1C are a set of images showing extraction and SDS-PAGE analysis of human and rat embryonic CSF. FIG. 1A is an image of hematoxylin and eosin sagittal section of E14.5 rat showing CSF aspiration technique and the position of the syringe needle relative to surrounding tissues in the lateral ventricle (LV) and the 4^thventricle (4^thV). The inset image of E14.5 rat embryo provides orientation. The arrow head is 4^thV, and the arrow is the mouth/chin. FIG. 1B shows CSF aspirated from the 4^thventricle of a CS20 human embryo (CS20) and a CS19 human embryo (CS19) separated by size using SDS-PAGE on a 7.5% or 10% polyacrylamide gel respectively. For clarity, the CS20 sample shows 1/7^thof the sample used in the final analysis. FIG. 1C shows CSF aspirated from the lateral ventricles (LV) of E12.5, E14.5 and E17.5 rat. The arrow in all samples represents Apolipoprotein-B.

FIGS. 2A-2D are graphs showing classification and comparison of proteins based on subcellular localization. Graphic representation of the subcellular localization of proteins in CS 20 embryonic human CSF (FIG. 2A), embryonic rat CSF (FIG. 2B), and E16.5 mouse brain (FIG. 2C) is shown. The percentage of protein localization is calculated based on the total number of proteins localized to each space divided by the total number of proteins in the CSF that we were able to assign localization (human CSF-187 proteins, rat CSF-137 proteins, and mouse brain-179 proteins). Some proteins were localized to multiple compartments within the cell. FIG. 2D shows a comparison between human CSF, rat CSF, and mouse brain of the number of protein from each category based on localization.

FIG. 3 is a graph showing comparison of proteins based on molecular function. Proteins present in embryonic human CSF, embryonic rat CSF, and embryonic mouse brain were analyzed using the Panther gene ontology database and classified according to molecular function. The chart includes protein category name. Percentage is calculated from the number of proteins assigned to each category over total number of proteins analyzed.

FIG. 4 is a graph showing comparison of proteins based on biological process. Proteins present in embryonic human CSF, embryonic rat CSF, and embryonic mouse brain were analyzed using the Panther gene ontology database and classified according to the biological process with which the proteins are involved. The chart includes protein category name. Percentage is calculated from number of proteins assigned to each category over total number of proteins analyzed.

FIGS. 5A-5C are graphs showing classification of proteins based on molecular function. Proteins present in embryonic human CSF (FIG. 5A), embryonic rat CSF (FIG. 5B), and embryonic mouse brain (FIG. 5C) were analyzed using the Panther gene ontology database and classified according to molecular function. Each graph includes protein category name, number of proteins assigned to each category, and percentage of proteins assigned to each category. Proteins can be assigned to more than one category based on molecular function.

FIGS. 6A-6C are graphs showing classification of proteins based on biological process. Proteins present in embryonic human CSF (FIG. 6A), embryonic rat CSF (FIG. 6B), and embryonic mouse brain (FIG. 6C) were analyzed using the Panther gene ontology database and classified according to the biological process the proteins are involved with. Each graph includes protein category name, number of proteins assigned to each category, and percentage of proteins assigned to each category. Proteins can be assigned to more than one category based on biological process.

FIG. 7 is a graph showing sub-classification of regulatory molecules based on molecular function. Regulatory molecules present in the embryonic human CSF, rat CSF, and embryonic mouse brain were further sub-classified based on molecular function. Although in FIG. 3 the percentage of regulatory molecules found in CSF and mouse brain appears similar, further sub-classification shows a distinct similarity in protein classes between CSF samples and a distinct difference in protein classes between CSF and brain samples.

FIG. 8 is a graph showing sub-classification of protein metabolism and modification based on biological process. Proteins involved in protein metabolism and modification present in the embryonic human CSF, rat CSF, and embryonic mouse brain were further sub-classified based on biological process. Although in FIG. 4 the percentage of proteins involved in protein metabolism and modification found in CSF and mouse brain appeared similar, further sub-classification clearly shows a distinct similarity in protein classes between CSF samples and a distinct difference in protein classes between CSF and brain samples.

FIGS. 9A-9Z shows that embryonic CSF supports cortical explant viability and growth and E17 CSF stimulates proliferation of neural progenitor cells in cortical explants and in cell culture. FIG. 9A is a schematic diagram of cortical explant dissections; 3-D image of E16 rat brain with dark box depicting region of dissection for explant. Cross section image of rat brain depicts medial and lateral border of explant dissection. Crossed arrows designate orientation of explant (E) on membrane with orienting cut at medial-caudal side (L-lateral, M-medial, C-caudal, R-rostral). FIGS. 9B-9D are images showing tissue stained with Hoechst (blue), anti-PH3 (red), and anti-Tuj1 (green). (FIG. 9B) E17 rat cortex; (FIGS. 9C and 9D) E16 explants grown for 24 hours in 100% embryonic CSF (e-CSF) and 100% artificial CSF (ACSF), respectively. Explants grown in 100% embryonic CSF in vitro maintain tissue histology similar to embryo in vivo. FIGS. 9E-9G show tissue stained with anti-BrdU (blue), anti-PH3 (red), anti-Tuj1 (green). (FIG. 9E) E17 rat cortex labeled with BrdU, mother was administered a bolus of BrdU (60 mg/kg) 3 hours prior to removing embryos. (FIGS. 9F-9G) E16 explant grown for 24 hours in 100% embryonic CSF and ACSF respectively. Explants were administered BrdU (20 uM) 30 minutes prior to fixation. Explants grown in 100% embryonic CSF incorporated BrdU after 24 hours in vitro indicating cells undergoing DNA synthesis. Survival and proliferation of the explants grown with embryonic CSF are indicated by immunoreactivity for phospho-Histone H3 (PH3, a marker of cell division) along the ventricular surface, BrdU incorporation (marking proliferating cells at the time of BrdU exposure) in the ventricular zone, and Tuj1-positive-staining neurons in the developing cortical plate. FIGS. 9H-9M show E16 explants cultured in 100% E13 or E17 CSF for 24 hours, (FIGS. 9H and 9I) stained with anti-PH3 (red) and Hoechst (blue) (FIGS. 9J and 9K) stained with anti-Vimentin 4A4 (green) and Hoechst (blue), (FIGS. 9L and 9M) merged images of anti-PH3 (red), anti-Vimentin 4A4 (green) and Hoechst (blue). FIG. 9N shows quantification of total PH3-positive-staining cells per explant grown with E13 and E17 CSF. The number of PH3-positive-staining cells is represented as mean±SEM. The number of PH3-positive-staining cells was significantly increased in explants cultured with E17 CSF compared to E13 CSF (Mann-Whitney; E17: 44.1±1.43; E13: 25±4.2; p<0.05; n=4). FIG. 9O shows quantification of PH3-positive-staining cells along the ventricle per explant grown with E13 and E17 CSF. The number of PH3-positive-staining cells along the ventricle was significantly increased in explants cultured with E17 CSF compared to E13 CSF (Mann-Whitney; E17: 32.3±0.79; E13: 12.8±3.9; p<0.05; n=4). FIG. 9P shows quantification of Vimentin 4A4-positive-staining cells per explant grown with E13 and E17 CSF. The number of Vimentin 4A4-positive-staining cells was significantly increased in explants cultured with E17 CSF compared to E13 CSF (Mann-Whitney; E17: 45.9±6.6; E13: 13.9±2.2, p<0.05; n=3). FIGS. 9Q-9Y show single cells from dissociated primary neurospheres grown in: (FIGS. 9Q, 9T, and 9W) 20% ACSF, (FIGS. 9R, 9U, and 9X) 20% E14 CSF, (FIGS. 9S, 9V, and 9Y) 20% E17 CSF for 9 DIV and stained with anti-GLAST, Hoechst, and merged images, respectively. Primary dissociated spheres grown in E17 CSF proliferate and form spheres of slowing dividing GLAST positive cells. FIG. 9Z shows quantification of average number of spheres per cm²formed in the various conditions at 9 DIV.

FIGS. 10A-10F show that e-CSF supports cortical explant survival. FIGS. 10A, 10C, and 10E show E16 explants grown for 24 hours in 100% e-CSF, and FIGS. 10B, 10D, and 10F show 100% artificial CSF (ACSF) and stained for early apoptotic cell death marker Cleaved Caspase 3 (CC3). Explants grown in 100% embryonic CSF has decreased CC3 stain compared to explants grown in ACSF. The embryonic CSF supports tissue viability and survival.

FIGS. 11A-11I show neural stem cells grown in embryonic CSF maintain undifferentiated state. FIGS. 11A-11C show dissociated cells from primary neurospheres cultured in E17 CSF for 10 DIV. Cells maintain GLAST-positive neural progenitors when cultured in embryonic CSF. FIGS. 11D-11F show dissociated cells from primary neurospheres cultured in E17 CSF for 5 DIV and then supplemented with EGF and FGF. GLAST-positive-staining cells cultured in E17 CSF maintain responsiveness to EGF and FGF suggesting that stem cells cultured in CSF maintain undifferentiated and uncommitted state. FIGS. 11G-11I show dissociated cells from primary neurospheres cultured in EGF and FGF for 10 DIV.

FIGS. 12A-12G show embryonic CSF maintains GLAST-positive-staining stem cells for 44 DIV. FIG. 12A-12D show dissociated cells from primary neurospheres cultured in E17 CSF for 44 DIV. Cells maintain GLAST-positive neural progenitors when cultured in embryonic CSF for extended periods of time. FIG. 12E shows quantification of number of spheres per cm²when cultured for 10 DIV versus 44 DIV. FIG. 12F shows quantification of relative colony size of spheres cultured for 10 DIV versus 44 DIV. FIG. 12G shows quantification of circularity of spheres cultured for 10 DIV versus 44 DIV.

FIG. 13A-13C show dynamic changes in CSF protein concentration and composition during development. FIG. 13A is agGraph of total CSF protein concentration collected from rats at various stages in development. FIG. 13B is a silver stain of CSF from different ages in development, revealing a dynamic fluid with numerous changes in protein composition over time. FIG. 13C is a western blot analysis of specific proteins identified in the embryonic CSF. CSF collected from various ages during development and immunoblotted with antibodies to Albumin, Transferrin, FGF2, EC-SOD, Cathepsin B, Cystatin C, Amyloid Precursor Protein (sAPP).

FIGS. 14A-14F show that embryonic CSF activates IGF1R and p-AKT signaling and provides a source of insulin signaling to progenitor cells along the ventricle in the cortex. FIG. 14A shows Igf2 peptides recognized by LC-MS/MS in E17 CSF (red). FIG. 14B shows that Igf2 levels are detectable by western blot at E13 and then decrease into adulthood. FIG. 14C shows an in situ hybridization for IGF2 at E14. c′ and c″ are magnified images showing IGF2 levels highest in leptomeninges and blood vessels within the cortex, FIG. 14D shows an in situ hybridization for IGF2 at E17. d′ and d″ are magnified images showing IGF2 levels are highest in the choroid plexus (CP), leptomeninges, and blood vessels within the cortex, FIG. 14E 10× and (e′) 20× image of IHC analysis of Igf1R localization in the E17 developing rat brain reveals Igf1R localization along the apical surface of the ventricle. FIG. 14F shows lysates of cortical cells treated with ACSF, E17 CSF, or IGF2 for 5 minutes immunoblotted with antibodies to p-IGF1R, p-AKT, AKT, P-ERK1/2, and ERK1/2.

FIGS. 15A-15K show that Igf2 maintains and stimulates proliferation of neural progenitor cells. FIGS. 15A-15D show single cells dissociated from primary neurospheres grown in control media or control media plus IGF2 (20 ng/ml). Small secondary spheres cultured with Igf2 alone form after 10 DIV. IHC with anti-GLAST on secondary spheres after 10 DIV shows GLAST immunoreactivity, indicating maintenance of neural progenitor cell identity with IGF2 alone. FIGS. 15E-15G show E16 cortical explants cultured in control E17 CSF or E17 CSF with IGF2 neutralizing antibody (IGF2 NAb), stained with anti-Vimentin 4A4 (green) and Hoechst (blue). FIG. 15G shows quantification of Vimentin 4A4-positive-staining cells per explant grown with E17 control CSF or with IGF2 NAb. The number of Vimentin 4A4-positive-staining cells was significantly decreased in explants cultured with E17 CSF plus IGF2 NAb compared to control E17 CSF (Mann-Whitney; E17 control mean: 28.8±4.3; E17 Igf2 neutralizing antibody mean: 13.9±2.0; n=4, p<0.05). FIGS. 15H-15J show E16 cortical explants cultured with Neural Basal Media plus ACSF (control) or with supplemental IGF2 stained with anti-Vimentin 4A4 (green) and Hoechst (blue). FIG. 15J shows quantification of Vimentin 4A4-positive-staining cells per explant grown with control media or with supplemental IGF2. The number of Vimentin 4A4-positive-staining cells was increased in explants cultured with IGF2 supplementation compared with control (Mann-Whitney; Igf2 supplementation mean: 36.7±2.1; control mean: 20.4±4.46; n=8, p<0.05). FIG. 15K shows an overall model depicting factors released from the choroid plexus into the CSF can act over large distances to regulation progenitor cell survival, proliferation and maintenance. As an example, we illustrate IGF2 as a secreted factor that regulates the maintenance of progenitor cell fate.

DETAILED DESCRIPTION

Here we undertake a systematic, detailed, and unbiased proteomic analysis of human e-CSF from Carnegie Stage 19-20 (approximately 48-51 days post ovulation). We also report an extensive proteome analysis of rat e-CSF from three different time points E12.5, E14.5, and E17.5 during cortical development and list all the proteins that are common among the three time points as well as those proteins that are different.

We report a list of the common proteins found between the human and rat e-CSF. Furthermore, using various gene ontology programs we categorize the proteins in the e-CSF and compare the subcellular localization, molecular function, and biological process of embryonic human and rat CSF. We find 130 proteins shared between the human and rat e-CSF and that there are many similarities in the categories of proteins found within the CSF based on molecular function and biological process. This systematic analysis of proteins common to many ages lays the groundwork for analysis of changing CSF components that may have more specific developmental roles.

As described herein, rat embryonic CSF proteome is a complex and dynamic milieu of extracellular matrix proteins, intracellular proteins, and signaling factors (see also, Zappaterra et al., J Proteome Res 6, 3537-48 (2007)). However, prior to the present invention, the direct influence of embryonic CSF on cortical progenitor cells had been challenging to assess due to the difficulty of obtaining substantial amounts of CSF. In addition, we developed a cortical explant culture system in which embryonic cortex dissected from a consistent location of the lateral wall is placed on polycarbonate membranes and floated on embryonic CSF (FIG. 9A). This explant culturing technique enables variable pairings of cortical tissue and CSF, or e-CSF components to investigate the relationship between cortical progenitor cells and CSF-mediated signaling.

On the basis of this work, the present invention features methods for isolation of e-CSF, and methods of culturing cells (e.g., stem cells or progenitor cells such as neural stem cells) using one or more (e.g., 2, 3, 4, 5, 6, 8, or 10) components (e.g., a purified or recombinantly produced polypeptide) of e-CSF and compositions including cells with one or more such components.

Isolation of CSF from Human Embryos

In general, CSF can be isolated from any mammalian embryo using the methods described herein. Typically the embryos at the appropriate stage are collected, and the extra embryonic membranes and tissues are dissected away in a buffer solution (e.g., phosphate-buffer saline (PBS) or Hanks' Balanced Salt Solution (HBSS)). A capillary needle (e.g., a syringe or microcapillary pipette) is placed into a CNS ventricle (e.g., lateral, third, or fourth ventricle) and the CSF is withdrawn. To avoid contaminating cells, it is desirable that contact with either blood vessels or with the neuroepithelium be avoid. To ensure that the e-CSF sample is cell-free, the sample can treated to remove cells (e.g., by centrifugation or by filtration).

In one example, human embryos were collected through the joint MRC-Wellcome Trust Human Developmental Biology Resource at the University of Newcastle, Institute of Human Genetics. The embryos at CS 19-20 were placed in ice-cold sterile Phosphate Buffered Saline (PBS) solution and all extra-embryonic membranes and tissues were removed. The embryos were washed in sterile PBS and carefully placed on the dissection platform under the microscope. A Hamilton syringe was placed carefully into the fourth ventricle and the CSF was collected paying close attention not to make contact with the neuroepithelium lining of the fourth ventricle. The samples used for analysis had no microscopically visible contaminating neuroepithelial cells or red blood cells. Nonetheless, the CSF samples were centrifuged at 10,000 g at 4° C. for 10 minutes to remove any intact contaminating cells and then were frozen at −80° C. until further analysis.

In another example, rat embryos (Sprague Dawley) at stage E12.5, E14.5 and E17.5 were removed from extra-embryonic membranes and tissues and placed in sterile Hanks Balanced Salt Solution (HBSS). Each embryo was handled individually and washed in HBSS, gently patted dry and placed on a microdissection tray. The CSF was carefully aspirated from each rat embryo under the microscope with a pulled tip glass microcapillary pipette (Drummond Scientific Company 20 μl). The needle was steadily held within the inside of the ventricle so as to prevent major contact with the neuroepithelial wall and the CSF was slowly aspirated. For E17.5, the embryo was placed on its back and the glass needle was inserted into the left lateral ventricle and then into the right lateral ventricle to collect the maximum amount of CSF from the lateral ventricles. For E12.5, the embryo was placed on its side and the glass needle was inserted directly into the lateral ventricle. Due to the patency of the neural tube at this stage, the CSF was collected from the developing lateral, third, and fourth ventricle. For E14.5, the embryo was also placed on its side and the glass needle was either inserted into the lateral ventricle or into the fourth ventricle and the CSF was collected from each location separately. FIG. 1A is a diagram depicting CSF isolation from E14.5 rat. CSF for each analysis was collected from two entire litters and pooled as one sample. To minimize protein degradation, CSF samples were kept at 4° C. during collection. CSF samples were centrifuged at 10,000 g at 4° C. for 10 minutes to remove any contaminating cells. The samples that we used for analysis had no visible sign of contaminating neuroepithelium cells or red blood cells as we could detect under the microscope. Samples were frozen at −80° C. until further analysis.

Cell Culture Using e-CSF Components

As we have shown that e-CSF stimulates proliferation and maintenance of neural stem cells in vitro and have, for the first time, identified many of the polypeptides found in e-CSF, it now becomes possible to uses these identified proteins in a cell culture system as proliferation, differentiation and maintenance, particularly with regard to stem cells and progenitors, especially those of neural origin. On this basis, the invention features methods of cell culture using e-CSF or one or more e-CSF components (e.g., polypeptides, either alone or as a supplement to standard cell culture media, and cell culture compositions including one or more components of e-CSF.

Any of the polypeptides identified in e-CSF may be used as a supplement in a cell culture (e.g., those described in Tables 1-4), or a combination of components from e-CSF (e.g., polypeptides) may be used for cell culture (e.g., for proliferating cells such as stem cells). Functional fragments (e.g., soluble fragments) of any of the proteins described herein) may be used in the invention. Soluble fragments are particularly useful for membrane bound proteins.

Cell Types

The compositions and methods of the invention may employ any type of cultured cell known in the art. In particular embodiments, a proliferating cell, such as a stem cell or progenitor cell is used. Neural cells (e.g., neural stem cells and neural progenitor cells) can be used in the invention as well. Human and non-human mammal cells (e.g., rat or mouse) cells are used in certain embodiments. Stem cell lines are, for example, commercially available or can be obtained directly from laboratory animals such as mice or rats.

Cell Media Compositions

In one embodiment, the cell media composition of the invention includes any medium known in the art supplemented with at least one (e.g., at least 2, 3, 4, 5, 6, 8, 10, 15, 25, 50) components identified in e-CSF (e.g., those described herein). Exemplary media types used for culturing neural stem cells include Neuralbasal Media (Invitrogen Corp.), Neural Stem Cell Commitment Media or Neural Stem Cell Growth Meida (AlphaGenix, Inc.), and NeuroCult NS-A Proliferation, Human; Kit or NeuroCult NS-A Differentiation Humam Kit (Stem Cell Technologies, Inc.). In another embodiment, the cell culture media is derived entirely from e-CSF components.

Determining Whether a Component of e-CSF Enhances Proliferation, Maintenance, or Differentiation of a Cell

Any component of e-CSF (e.g., those described herein) may be analyzed to determine its effect on a cell (e.g., a neuronal progenitor or stem cell described herein) in culture. Desirable components are those which result in maintenance of the cells or those that result in more rapid proliferation or differentiation of the cell. Assays to measure proliferation (e.g., using cell dyes or incorporation of a modified nucleotide such as BrdU) and differention (e.g., using differentiation markers known in the art) are well known by those of skill in the art.

The Role of e-CSF in Brain Development

Embryonic CSF plays a fundamental, dynamic role in defining an endogenous niche for the survival and proliferation of cortical neural progenitors. CSF alone supports the growth and proliferation of cortical explants in the absence of exogenous media or factors. CSF from different ages in development harbors distinct proliferative capacities for neural progenitor cells the characterization of the CSF proteome has identified several classes of proteins in the CSF established as essential regulators of proliferation and maintenance of neuronal progenitor cells; and we identified and characterized a novel role for IGF2 signaling in the embryonic CSF as a regulator of cortical progenitor cell proliferation.

The CSF has been traditionally considered as a fluid cushion that bathes the central nervous system, acting as a passive sink for biomarkers of central nervous system function and pathology. Collectively, our study represents a paradigm shift in developmental neuroscience, suggesting that the embryonic CSF proteome as a dynamic milieu of growth-promoting signals for neural stem cells (FIG. 15K).

The CSF-choroid-plexus system is ideally suited to act as a rapid, spatially synchronized medium for triggering local and global changes in molecular signaling. Dynamism of factors such as IGF2 levels in the CSF is consistent with a role of the CSF as a vehicle for orchestrating cortical neurogenesis: IGF2 expression increases during development, is maximal during the peak of cortical neurogenesis in the rat brain (E17-E19), and declines as cortical neurogenesis nears completion around birth. Therefore, the appearance of signaling factors such as IGF2 stimulate the proliferation of cortical progenitors to maintain them in an uncommitted state through development. Igf2 and other molecules (Martin et al., Dev Biol 297, 402-16 (2006)) appear to be released in the CSF by choroid plexus, which appears in the lateral ventricles between E13 and E15. These signaling molecules, via the CSF must act widely on cortical precursors that, in the case of the embryonic human brain, may be centimeters away from the source of the factor. It is unclear whether there is a gradient of Igf2 in the embryonic CSF influences regional differences in proliferation across the cortical mantle (Bayer et al., Prog Neurobiol 29, 57-106 (1987)), as has been shown for Slit in the adult CSF (Sawamoto et al., Science 311, 629-32 (2006)), or whether ciliary movement or diffusion through a far smaller volume equilibrates Igf2 concentration in the embryonic ventricles. A fundamental aspect of neural differentiation may be the simple isolation of developing cells from this growth-promoting environment, by the withdrawal of the ventricular process (Cappello et al., Nat Neurosci 9, 1099-107 (2006)).

Our findings have several important implications. First, CSF in the embryo and the adult is a dynamic fluid that contacts a number of CNS precursors as well as differentiating neurons and glia. Second, CSF components can be dispersed over large areas and thus may be more significant and pervasive regulators of development, stem cell renewal, disease, neurodegeneration and behavior than previously thought. Third, since the CNS represents just one example of an epithelium that grows in relation to an extracellular fluid, our findings may generalize to other epithelia which are likely to develop using similar rules, with a major contributor to the “stem cell niche” being the fluid that bathes the epithelium (Bendall et al., Nature 448, 1015-21 (2007)), similar to the microenvironment that invests hematopoietic stem cells, of which Igf2 is also an essential component (Zhang et al., Blood 103, 2513-21 (2004); Orkin et al., Cell 132, 631-44 (2008)), as in the embryonic CSF. Finally, if a major component of the stem cell niche reflects secreted factors acting at large distances from their sources, a deeper understanding of the proteomic composition of extracellular fluids may provide unexpected ways to regulate stem cell behavior.

In-Gel Digestion and Mass Spectrometry

To determine the protein contents of e-CSF, frozen CSF samples were thawed on ice. Sample buffer was added and the samples were boiled for 5 minutes and subjected to SDS-PAGE using either 10% or 7.5% polyacrylamide (37.5:1 acrylamide:bis-acrylamide) gels as indicated in FIG. 1B-1C. Each gel lane (which included the 4.2% polyacrylamide stacking gel) was cut into ten regions and each region was diced and subjected to in-gel digestion with sequencing grade modified trypsin (Promega, 6 ng/μl) in 50 mM ammonium bicarbonate overnight at 37° C. Peptides were extracted with 50% acetonitrile (MeCN), 2.5% formic acid (FA) and then dried. Peptides were then resuspended in 2.5% MeCN, 2.5% FA and loaded using an autosampler onto a microcapillary column packed with 12 cm of reverse phase MagicC18 material (5 μm, 200 Å, Michrom Bioresources, Inc.). Elution was performed with a 5-35% MeCN (0.1% FA) gradient over 60 minutes, after a 15 minute isocratic loading at 2.5% MeCN, 0.5% FA. Mass spectra were acquired in LTQ and LTQ-XL linear ion trap mass spectrometers (Termo Electron) over the entire 75 minutes using ten MS/MS scans following each survey scan. Raw data were searched against either the human or rat IPI forward and reverse concatenated databases using Sequest software requiring tryptic peptide matches with a 2 Da mass tolerance (Elias et al., Nat Methods, 2005. 2:667-75). Cysteine residues were required to have a static increase in 71.0 Da for acrylamide adduction and differential modification of 16.0 Da on methionine residues was permitted. The resultant top matches for all analyses of each gel lane were compiled. Each list was then filtered independently using a dCn2 score of 0.2 and Xcorr scores of 1.8, 2.0 and 2.5 for singly, doubly, and tripled charged ions respectively. Proteins on these filtered lists that had two or more peptides were retained. However keratin proteins were removed as they are known contaminants in most gel-based proteomic analyses. Based on the number of reverse database false-positives that were also retained following these filtering criteria, we estimate the following false-positive rates for the proteins in each sample: rat E12.5 lateral ventricle (LV), 0.45%; rat E14.5LV, 0.30%; rat E17.5LV, 0.50%; rat E14.5 4^thventricle, <0.00%; and human CS 20, <0.00%. For the human CS 19 sample the estimated false-positive rate for proteins identified by more than three peptides is <0.00%. The dataset of proteins for the embryonic mouse brain was extracted from LC-MS/MS data collected from 16 strong cation exchange (SCX) fractions generated during our previous study of the forebrain and midbrain extracts of E16.5 mouse embryos (Ballif et al., Mol Cell Proteomics, 2004. 3:1093-101). We compiled the LC-MS/MS data from four SCX fractions in the middle of the SCX gradient (not enriched for phosphopeptides) from each of the four regions of the gel and the top 200 identified proteins were subjected to further analysis.

Analysis of the Human Embryonic Proteome

Human CSF was collected from the fourth ventricle, as mentioned above, from two independent embryos at Carnegie Stage (CS) 19-20. From the first embryo (CS19) a total of 15 μl was collected, and from the second embryo (CS20) a total of 70 μl was collected. The CSF from these two independent samples was separated by I-D SDS-PAGE; FIG. 1B shows the Coomassie stained protein pattern of the CSF from CS20 and CS19 embryos run on 7.5% and 10% polyacrylamide gels, respectively. The two human e-CSF samples were analyzed separately. Table 1 shows the proteomic analysis of the CSF collected from the CS20 embryo and lists 188 proteins with 2 or more peptides identified. Using a number of protein analysis programs such as UniProt, Gene Ontology™ (GO), and the PANTHER (Protein Analysis Through Evolutionary Relationships) classification system we categorized the proteins found from the mass spectrometry data and list subcellular localization, protein function, tissue specificity, and relevant notes pertaining to each protein (Table 1) (Ballif et al., Mol Cell Proteomics, 2004. 3:1093-101). Analysis of the CSF from the CS19 human sample revealed 772 proteins with more than three peptides identified. The search results from this analysis suggested the presence of a number of non-CSF contaminants including 7 different mitochondrial specific precursor proteins such as the mitochondrial precursors for 4-aminobutyrate aminotransferase, fumarate hydratase, and isoform dut-M of deoxyuridine 5′-triphosphate nucleotidohydrolase, whereas no mitochondrial precursor proteins were identified in the rat CSF or in the CS20 human CSF sample. Therefore, the CS19 list was not further considered in the comparison to rat CSF. However, the proteins from this analysis are presented in Table 2 as this list is certainly enriched for human e-CSF proteins. The substantial differences between this sample and the other human and rat samples suggest that this sample contained multiple impurities, likely from lysed blood and/or neuroepithelial cells. Nonetheless, the differences highlight that the MS analysis is highly sensitive to contaminants, and that the absence of mitochondrial proteins in other samples indicates, that they are probably quite pure.

TABLE 1

Protein list of mass spectrometry analysis of Carnegie Stage 20 embryonic human CSF.

Number

of unique
Total

peptides
number

from
of
Accession

Subcellular

Found in Rat

protein
peptides
Number
Name of Protein
MW
location
Function
Tissue Specificity
Notes
CSF

206
584
P04114
APOLIPOPROTEIN B-100
515563
Secreted
Lipid and fatty acid
Plasma
Apo B-100 functions as a recognition
Yes - all rat

PRECURSOR

transport and

signal for the cellular binding and
samples

metabolism

internalization of LDL particles by the

apoB/E receptor

43
79
P02751
ISOFORM 1 OF
262607
Secreted,
Cell adhesion, cell
Plasma
Integrin signaling pathway
Yes - all rat

FIBRONECTIN

extracellular
motility, wound
fibronectin made

samples

PRECURSOR

space,
healing, and
by liver and

extracellular
maintenance of cell
cellular

matrix
shape
fibronectin made

by fibroblasts,

epithelial and

other cell types is

deposited in the

extracellular

matrix

42
114
P01023
ALPHA-2-
163278
Secreted
Serine Protease
Plasma
Contrary to the rat protein, which is an
Yes - all rat

MACROGLOBULIN

Inhibitor

acute phase protein, this protein is
samples

PRECURSOR

always present at high levels in

circulation

27
63
P02787
SEROTRANSFERRIN
77050
Secreted
Transport,
Plasma
Transport of iron from sites of
Yes - all rat

PRECURSOR

Transfer/Carrier

absorption and heme degradation to
samples

those of storage and utilization.

Serum transferrin may also play a role

in stimulating cell proliferation

25
91
Q5D0D7
ALB (Albumin) PROTEIN
71705
Secreted
Transport,
Plasma
Serum albumin, the main plasma
Yes - all rat

Transfer/Carrier

protein, has a good binding capacity
samples

for water, calcium, sodium,

potassium, fatty acids, hormones,

bilirubin and drugs. Its main function

is the regulation of the colloidal

osmotic pressure of blood

23
34
P01024
COMPLEMENT
187306
Secreted
Complement
Plasma
Plays a central role in the activation of
Yes - all rat

COMPONENT 3

mediated immunity

the complement system
samples

PRECURSOR

21
87
P02771
ALPHA-FETOPROTEIN
68678
Secreted
Transport,
Plasma
Binds copper, nickel, and fatty acids
Yes - all rat

PRECURSOR

Transfer/Carrier

as well as, and bilirubin less well than,
samples

serum albumin

19
45
P19823
INTER-ALPHA-TRYPSIN
106436
Secreted
Serine Protease
Plasma, widely
May act as a carrier of hyaluronan in
Yes - present in

INHIBITOR HEAVY CHAIN

Inhibitor
distributed
serum or as a binding protein
rat E14.5,

H2 PRECURSOR

between hyaluronan and other matrix
E17.5LV

protein, including those on cell

surfaces in tissues to regulate the

localization, synthesis and

degradation of hyaluronan which are

essential to cells undergoing

biological processes.

14
14
P49327
FATTY ACID SYNTHASE
273400
Cytoplasm
Lipid and fatty acid
Ubiquitous.
Involved in catalyzing the formation of
Yes - all rat

biosynthesis
Prominent
long chain fatty acids
samples

expression in

brain, lung, and

liver

14
15
P24821
ISOFORM 1 OF
240866
Secreted,
Cell adhesion,
Widely
Extracellular matrix protein implicated
Yes - all rat

TENASCIN PRECURSOR

extracellular
extracellular matrix
distributed
in guidance of migrating neurons as
samples

space,
glycoprotein-

well as axons during development,

extracellular
mediated signaling

synaptic plasticity as well as neuronal

matrix

regeneration. Ligand for integrin

receptors.

13
25
P19827
INTER-ALPHA-TRYPSIN
101389
Secreted
Protease Inhibitor
Plasma
See notes on INTER-ALPHA-
Similar to inter-

INHIBITOR HEAVY CHAIN

TRYPSIN INHIBITOR HEAVY CHAIN
alpha-inhibitor

H1 PRECURSOR

H2 PRECURSOR above.
H4 heavy chain

12
17
P01031
COMPLEMENT C5
188331
Secreted
Complement
Plasma
Activation of C5 by a C5 convertase
Yes - present in

PRECURSOR

mediated immunity

initiates the spontaneous assembly of
E17.5LV

the late complement components, C5-C9,

into the membrane attack

complex.

12
19
P02671
ISOFORM 1 OF
94973
Secreted
Blood clotting
Plasma
Monomers polymerize into fibrin and
Yes - all rat

FIBRINOGEN ALPHA

also acts as a cofactor in platelet
samples

CHAIN PRECURSOR

aggregation.

11
27
P02679
ISOFORM GAMMA-B OF
51512
Secreted
Blood clotting
Plasma
Monomers polymerize into fibrin and
Yes - all rat

FIBRINOGEN GAMMA

also acts as a cofactor in platelet
samples

CHAIN PRECURSOR

aggregation.

11
12
P12111
ALPHA 3 TYPE VI
343669
Secreted,
Cell adhesion - Cell
Widley
Collagen VI acts as a cell-binding
Yes - present in

COLLAGEN ISOFORM 1

extracellular
structure
distributed,
protein
rat E14.5,

PRECURSOR

space,

expressed in

E17.5LV

extracellular

muscles

matrix

10
19
P02647
APOLIPOPROTEIN A-I
30778
Secreted
Lipid and fatty acid
Major protein of
Participates in the reverse transport of
Yes - all rat

PRECURSOR

transport and
plasma HDL,
cholesterol from tissues to the liver for
samples

metabolism
also found in
excretion by promoting cholesterol

chylomicrons.
efflux from tissues.

10
13
P06396
ISOFORM 1 OF
85698
Secreted
Actin remodeling -
Plasma
Calcium-regulated, actin-modulating
Yes - all rat

GELSOLIN PRECURSOR

Cell structure

protein. It can promote the assembly
samples

of monomers into filaments as well as

sever filaments already formed. May

be involved in myelination.

10
16
P05155
PLASMA PROTEASE C1
55154
Secreted
Serine Protease
Plasma
May regulate complement activation,
Yes - all rat

INHIBITOR PRECURSOR

Inhibitor

blood coagulation, fibrinolysis and the
samples

generation of kinins.

10
12
P0C0L4
COMPLEMENT C4-A
192771
Secreted
Complement
Plasma
Inflammatory response
Yes - all rat

PRECURSOR

mediated immunity

samples

10
10
Q13822
ISOFORM 1 OF
99004
Membrane
Hydrolase
Predominantly
Localized in secretory epithelial cells
Yes - all rat

ECTONUCLEOTIDE

expressed in
in the brain and the eye including
samples

PYROPHOSPHATASE/

brain, placenta,
choroid plexus epithelial cells, ciliary

PHOSPHODIESTERASE 2

ovary, and small
epithelial cells, iris pigment epithelial

intestine.
cells, and retinal pigment cells. Has a

potent tumor cell motility-stimulating

activity.

9
16
P02675
FIBRINOGEN BETA
55928
Secreted
Blood clotting
Plasma
Monomers polymerize into fibrin and
Yes - all rat

CHAIN PRECURSOR

also acts as a cofactor in platelet
samples

aggregation.

9
22
P02766
TRANSTHYRETIN
15887
Secreted
Hormone transport
Most abundant in
Thyroid hormone-binding protein.
Yes - all rat

PRECURSOR

the choroid
Probably transports thyroxine from the
samples

plexus. Also
bloodstream to the brain.

present in the

liver

8
17
P01009
ALPHA-1-ANTITRYPSIN
46737
Secreted
Serine Protease
Plasma
Belongs to the serpin family
Yes - all rat

PRECURSOR

Inhibitor

samples

8
21
P02760
AMBP PROTEIN
38999
Secreted
Serine Protease
Plasma, urine,
It appears not only as a free monomer
Yes - all rat

PRECURSOR

Inhibitor
and
but also in complexes with IgA and
samples

cerebrospinal
albumin.

fluid

8
11
P23471
ISOFORM SHORT OF
163444
Membrane
Cell surface
Central nervous
May be involved in the regulation of
Yes - all rat

RECEPTOR-TYPE

receptor mediated
system
specific developmental processes in
samples

TYROSINE-PROTEIN

signal transduction,

the CNS

PHOSPHATASE ZETA

transmembrane

PRECURSOR

receptor protein

tyrosine

phosphatase activity

8
8
P08238
HYPOTHETICAL
84843
Cytoplasm
Chaperone, protein
Ubiquitous
Belongs to the heat shock protein 90
Yes - all rat

PROTEIN

folding, stress

family
samples

DKFZP761K0511 - heat

response

shock 90 kDa protein 1,

beta

8
15
Q9P173
PRO2275 - Serpin
13097
Secreted
Serine Protease
Plasma
Belongs to the serpin family

peptidase inhibitor, clade A

Inhibitor - Blood

(alpha-1 antiproteinase,

coagulation

antitrypsin), member 1

7
9
P00450
CERULOPLASMIN
122205
Secreted
Transport,
Plasma
It is involved in iron transport across
Yes - similar to

PRECURSOR

Transfer/Carrier,

the cell membrane, and metal ion
GPI anchored

Oxidoreductase

oxidoreductase activity
ceruloplamsin

present in all rat

samples

7
8
N/A
45 KDA PROTEIN -
44847
Secreted
Transport, Transfer
Plasma, widely
Involved in phospholipid transfer in
Yes - all rat

Homologous to

Carries
distributed
the serum.
samples

Phospholipid transfer

protein

7
10
P19022
CADHERIN-2
99851
Membrane
Cell adhesion
Widely
May be involved in neuronal tissue
Yes - neural

PRECURSOR (Neuronal

distributed
recognition
cadherin

cadherin)

precursor

present in all rat

samples

7
7
P78527
ISOFORM 1 OF DNA-
469089
Nucleus
Serine/threonine-
Ubiquitous
Molecular sensor for DNA damage

DEPENDENT PROTEIN

protein kinase

KINASE CATALYTIC

SUBUNIT

7
7
Q5CAQ7
HEAT SHOCK PROTEIN
98113
Cytoplasm
Molecular
Ubiquitous
Belongs to the heat shock protein 90
Yes - HSP 90

HSP 90-ALPHA 2

chaperone, protein

family
family present in

folding, stress

all rat samples

response

7
7
P35580
MYOSIN-10
228939
Intracellular
Cell structure, cell
Brain
Actin binding motor protein
Yes - all rat

motility

samples

7
16
P20742
PREGNANCY ZONE
163836
Secreted
Protease Inhibitor
Plasma
Belongs to the protease inhibitor I39
Yes - alpha-1-

PROTEIN PRECURSOR

(alpha-2-macroglobulin) family
macroglobulin

present in all rat

samples

6
10
P02649
APOLIPOPROTEIN E
36154
Secreted
Lipid and fatty acid
Plasma
Mediates the binding, internalization,
Yes - all rat

PRECURSOR

transport and

and catabolism of lipoprotein
samples

metabolism

particles.

6
6
P46821
MICROTUBULE-
270620
Intracellular
Cell structure
Brain
The function of brain MAPS is
Yes - present in

ASSOCIATED PROTEIN

unknown. Phosphorylated MAP1B
rat E12.5LV,

1B

may play a role in the cytoskeletal
E17.5LV

changes that accompany neurite

extension.

6
6
P06681
COMPLEMENT C2
83268
Secreted
Serine protease,
Plasma
Belongs to the peptidase S1 family.
Yes - all rat

PRECURSOR

complement-

samples

(FRAGMENT)

mediated immunity

6
6
P11047
LAMININ GAMMA-1
177607
Secreted,
Extracellular matrix
Basement
Binds to cells and is thought to
Yes - present in

CHAIN PRECURSOR

extracellular
linker protein-
membranes
mediate the attachment, migration
rat E12.5LV,

(Laminin B2 chain)

space,
mediated signaling

and organization of cells into tissues
E14.5

extracellular

during embryonic development by

matrix

interacting with other extracellular

matrix components.

6
7
P01019
ANGIOTENSINOGEN
53154
Secreted
Serine Protease
Plasma
Belongs to the serpin family. Helps
Yes - all rat

PRECURSOR

Inhibitor

regulate volume and mineral balance
samples

of body fluids.

6
7
P33151
CADHERIN-5
87516
Membrane
Cell adhesion
Endothelial
Plays a role in endothelial adhesion.
Yes - all rat

PRECURSOR

tissues and brain

samples

6
7
P98160
BASEMENT MEMBRANE-
468825
Secreted,
Cell adhesion
Widely
Integral component of basement
Yes - all rat

SPECIFIC HEPARAN

extracellular

distributed in
membranes.
samples

SULFATE

space,

various tissues

PROTEOGLYCAN CORE

extracellular

PROTEIN PRECURSOR

matrix

(Perlecan)

6
10
O00468
AGRIN PRECURSOR
214889
Secreted,
Cell adhesion
Embryonic
Component of the basal lamina that
Yes - all rat

extracellular
mediated signaling
nervous system
binds to laminin.
samples

space,

and muscle.

extracellular

matrix

5
7
Q08380
GALECTIN-3-BINDING
65331
Secreted,
Intergrin-mediated
Ubiquitous.
May stimulate host defense against

PROTEIN PRECURSOR

extracellular
cell adhesion
Detected in body
viruses and tumor cells.

space,

fluids such as

extracellular

semen, milk,

matrix

serum, tears,

saliva and urine.

5
5
Q14624
ISOFORM 2 OF INTER-
101242
Secreted
Protease Inhibitor
Plasma
May be involved in acute phase
Yes - all rat

ALPHA-TRYPSIN

reactions.
samples

INHIBITOR HEAVY CHAIN

H4 PRECURSOR

5
5
P35579
MYOSIN-9
226401
Intracellular
Cytokinesis, cell
In the kidney,
Non-muscle myosin involved in a
Yes - present in

shape, and
expressed in the
number of cellular functions.
12.5LV,

specialized
glomeruli. Also

E14.5LV,

functions such as
expressed in

E17.5LV

secretion and
leukocytes.

capping

5
7
P04217
ALPHA-1B-
54273
Secreted
Function unknown
Plasma
Immunoglobulin domain glycoprotein
Yes - present in

GLYCOPROTEIN

E12.5LV, E14.5

PRECURSOR

5
5
P13611
ISOFORM V0 OF
372820
Secreted,
Extracellular matrix
Brain
Also known as chondroitin sulfate
Yes - all rat

VERSICAN CORE

extracellular
protein-mediated

proteoglycan core protein 2
samples

PROTEIN PRECURSOR

space,
signaling, cell

extracellular
adhesion, cell

matrix
motility

5
7
P23142
ISOFORM B OF FIBULIN-1
77186
Secreted,
Cell adhesion, cell
Widely
Incorporated into fibronectin-
Yes - all rat

PRECURSOR

extracellular
motility
expressed during
containing matrix fibers. May play a
samples

space,

embryonic
role in cell adhesion and migration

extracellular

development.
along protein fibers within the

matrix

extracellular matrix. Could play a

significant role in modulating the

neurotrophic activities of APP,

particularly soluble APP.

5
7
P36955
PIGMENT EPITHELIUM-
46342
Secreted
Serpin family
Retinal pigment
Pigment epithelium-derived factor, a

DERIVED FACTOR

member -
epithelial cells,
neurotrophic protein, is a member

PRECURSOR (PEDF)

neurotrophic
adult murine
serpin that has been shown to

properties,
SVZ cells, and
promote the survival and/or

antiangiogenic
blood plasma
differentiation of rat cerebellar granule

neurons and human retinoblastoma

cells in vitro. Also PEDF was shown

to prevent the death and atrophy of

spinal motor neurons in the

developing neonatal mouse after

axotomy. PEDF is also secreted by

retinal pigment epithelial cells into the

interphotoreceptor matrix, where it

acts on photoreceptor cells.

5
6
P55287
ISOFORM 2 OF
76541
Membrane
Cell adhesion
Expressed
Also known as osteoblast-cadherin
Yes - all rat

CADHERIN-11

mainly in brain

samples

PRECURSOR

but also found in

other tissues.

Expressed in

neuroblasts

5
5
P02008
HEMOGLOBIN SUBUNIT
15506
Intracellular
Oxygen Transport,
Red blood cells
The zeta chain is an alpha-type chain

ZETA

Transfer - Carrier

of mammalian embryonic hemoglobin,

Protein

synthesized primarily in the yolk sac.

5
5
O00533
ISOFORM 2 OF NEURAL
136654
Membrane
Extracellular matrix
Expressed in the
Plays a role in nervous system

CELL ADHESION

protein-mediated
fetal and adult
development. Plays important roles in

MOLECULE L1-LIKE

signaling, cell
brain
neurite outgrowth and neuronal

PROTEIN PRECURSOR

adhesion, cell

survival.

motility

5
6
P48681
NESTIN
176706
Intracellular
Cell structure
CNS stem cells.
Upon terminal neural differentiation,
Yes - present in

nestin is down-regulated and replaced
E12.5LV,

by neurofilaments.
E14.5LV,

E17.5LV

5
7
P00751
ISOFORM 1 OF
85533
Secreted
Serine Protease -
Plasma
Hydrolase, Peptidase
Yes - all rat

COMPLEMENT FACTOR

Complement

samples

B PRECURSOR

Mediated Immunity

(FRAGMENT)

4
5
Q00839
ISOFORM SHORT OF
88814
Nucleus. Cell
DNA and RNA
Ubiquitous
Invovled in RNA splicing
Yes - all rat

HETEROGENEOUS

surface
binding and RNA

samples

NUCLEAR

processing.

RIBONUCLEOPROTEIN U

4
4
P12277
CREATINE KINASE B-
42644
Membrane
Kinase, energy
Ubiquitous
Plays a central role in tissues with
Yes - all rat

TYPE

modulation

variable energy demands.
samples

4
4
P34932
HEAT SHOCK 70 KDA
94300
Intracellular -
Heat shock, protein
Ubiquitous
Heat shock protein 70 family
Yes - all rat

PROTEIN 4

Cytoplasm
folding, stress

samples

response

4
4
P13010
ATP-DEPENDENT DNA
82573
Nucleus
Single stranded
Ubiquitous
Plays a role in chromosome

HELICASE 2 SUBUNIT 2

ATP-dependent

translocation. Involved in DNA

DNA helicase

nonhomologous end joining required

activity

for double-strand break repair and

V(D)J recombination.

4
4
Q9BQ02
NCL (Nucleolin) PROTEIN
51641
Nucleus
Major nucleolar
Ubiquitous
It is thought to play a role in pre-rRNA
Yes - present in

protein of growing

transcription and ribosome assembly
E12.5LV,

eukaryotic cells.

E17.5LV

Nucleotide binding

4
4
P49321
ISOFORM 1 OF NUCLEAR
85238
Intracellular -
Nuclear-cytoplasmic
Testis and
Required for DNA replication, normal
Yes - all rat

AUTOANTIGENIC SPERM

Nucleus,
shuttling
Sperm
cell cycle progression and cell
samples

PROTEIN

Cytoplasm

proliferation.

4
10
P07477
TRYPSIN PRECURSOR
26558
Secreted,
Serine protease
Ubiquitous
Belongs to the peptidase S1 family

(EC

extracellular

3.4.21.4) > PIR1:TRPGTR

space,

trypsin (EC 3.4.21.4)

extracellular

matrix

4
7
Q7KZ97
ANTITHROMBIN III
52692
Secreted
Serine Protease
Plasma
Regulates the blood coagulation

VARIANT

Inhibitor

cascade. Belongs to the serpin family.

4
9
P04004
VITRONECTIN
54306
Secreted,
Cell adhesion
Plasma
Vitronectin is an abundant

PRECURSOR

extracellular

glycoprotein found in blood plasma

space,

and the extracellular matrix. It

extracellular

regulates proteolysis initiated by

matrix

plasminogen activation. It is

recognized by certain members of the

integrin family and serves as a cell-to-

substrate adhesion molecule.

4
4
Q99435
PROTEIN KINASE C-
91346
Secreted
Cell communication,
Widely
Regulation of growth and
Yes - all rat

BINDING PROTEIN NELL2

Cell adhesion, Cell
distributed
neurogenesis
samples

PRECURSOR

structure

4
4
P43146
NETRIN RECEPTOR DCC
158457
Membrane
Ligand mediated
Axons of the
Axon guidance and neuronal growth
Yes - all rat

PRECURSOR

signaling, cell
central and
cone growth
samples

adhesion
peripheral

nervous system

and in

differentiated cell

types of the

intestine

4
4
Q02246
CONTACTIN-2
113393
Membrane;
Cell Adhesion -
Highly expressed
May play a role in the initial growth
Yes - all rat

PRECURSOR

(attached to the
Neurogenesis
in brain
and guidance of axons. Belongs to
samples

neuronal

the immunoglobulin superfamily.

membrane by a

GPI-anchor and

is also released

from neurons)

4
5
O75882
ISOFORM 1 OF
158537
Membrane
Protease involved in
Secreted by
May regulate chemotactic activity of
Yes - all rat

ATTRACTIN

cell clustering
activated T
chemokines. Has a critical role in
samples

PRECURSOR

during inflammatory
lymphocytes.
normal myelination in the central

response
Also expressed
nervous system.

in peripheral

blood leukocytes.

spleen, lymph

node, tonsil,

bone marrow

and fetal liver.

4
6
Q96PQ8
FACTOR VII ACTIVE SITE
75553
Secreted
Serine Protease -
Plasma
Involved in blood coagulation,

MUTANT

Blood clotting

activated by Factor Xa

IMMUNOCONJUGATE

4
5
O00391
QUIESCIN Q6 ISOFORM A
82578
Membrane
Oxidase
Widely
May contribute to disulfide bond
Yes - all rat

distributed,
formation in a variety of secreted
samples

expressed in
proteins. Induced in quiescent cells.

heart, placenta,

lung, liver,

skeletal muscle,

pancreas and

very weakly in

brain and kidney

4
7
P02461
COLLAGEN ALPHA-1(III)
138555
Secreted,
Cell adhesion - Cell
Widely
Present in most soft connective tissue
Yes - all rat

CHAIN PRECURSOR

extracellular
structure
distributed,

samples

space,

highly expressed

extracellular

in skin, lungs,

matrix

intestinal walls,

and blood

vessels

4
6
Q4KKU6
DESMOGLEIN 2
122294
Membrane
Cell adhesion
Widley
Component of desmosomes

distributed

4
4
Q92945
FAR UPSTREAM
72709
Intracellular -
RNA binding, RNA
Detected in

Yes - present in

ELEMENT-BINDING

Nucleus,
processing and may
neural and non-

E12.5LV,

PROTEIN 2

Cytoplasm
play a role in mRNA
neural cell lines.

E14.5LV,

trafficking

E17.5LV

4
7
P05546
HEPARIN COFACTOR 2
60178
Secreted
Thrombin inhibitor
Expressed
Belongs to the serpin family.
Yes - present in

PRECURSOR

involved in blood
predominantly in

E14.5 4thV,

clotting - Serine
liver.

E17.5LV

Protease Inhibitor

4
4
Q5HY53
FILAMIN A, ALPHA
280018
Intracellular
Cell structure, cell
Ubiquitous
Actin binding protein
Yes - all rat

motility

samples

4
4
Q86VP6
ISOFORM 1 OF CULLIN-
136376
Nucleus
Transcriptional
Widely
Down-regulates ubiquitination of
Yes - all rat

ASSOCIATED NEDD8-

Enhancer
distributed,
target proteins.
samples

DISSOCIATED PROTEIN 1

highly expressed

in lung

fibroblasts

4
5
Q92859
ISOFORM 1 OF
159959
Membrane
Cell adhesion
Widely
May be involved as a regulatory
Yes - all rat

NEOGENIN PRECURSOR

expressed
protein in the transition of
samples

undifferentiated proliferating cells to

their differentiated state. Belongs to

the immunoglobulin superfamily.

4
4
P00747
PLASMINOGEN
90569
Secreted
Protease
Present in
Plasmin dissolves the fibrin of blood
Yes - all rat

PRECURSOR

plasma and
clots and acts as a proteolytic factor in
samples

many other
a variety of other processes including

extracellular
embryonic development, tissue

fluids
remodeling, tumor invasion, and

inflammation.

3
8
P02753
PLASMA RETINOL-
23010
Secreted
Vitamin/Co-factor
Plasma
Delivers retinol from the liver stores to
Yes - all rat

BINDING PROTEIN

transport

the peripheral tissues. In plasma, the
samples

PRECURSOR

RBP-retinol complex interacts with

transthyretin, this prevents its loss by

filtration through the kidney glomeruli.

3
4
P06727
APOLIPOPROTEIN A-IV
45399
Secreted
Lipid and fatty acid
Plasma
May have a role in chylomicrons and
Yes - all rat

PRECURSOR

transport and

VLDL secretion and catabolism.
samples

metabolism

Apoa-IV is a major component of HDL

and chylomicrons.

3
3
O14980
EXPORTIN-1
123386
Intracellular -
Nuclear export of
Ubiquitous
Mediates nuclear export signal
Yes - present in

Nucleus,
cellular proteins and

dependent protein transport
E12.5LV,

Cytoplasm
RNAs

E14.5LV,

E17.5LV

3
3
P20908
COLLAGEN ALPHA-1(V)
183560
Secreted,
Cell adhesion - Cell
Ubiquitous
Component of connective tissue
Yes - all rat

CHAIN PRECURSOR

extracellular
structure

samples

space,

extracellular

matrix

3
3
P07942
LAMININ BETA-1 CHAIN
198066
Secreted,
Extracellular matrix
Widely
Is thought to mediate the attachment,
Yes - all rat

PRECURSOR

extracellular
linker protein-
distributed in
migration and organization of cells
samples

space,
mediated signaling
basement
into tissues during embryonic

extracellular

membranes
development by interacting with other

matrix

extracellular matrix components.

3
3
Q14974
IMPORTIN BETA-1
97170
Intracellular -
Nuclear protein
Ubiquitous
Import of proteins with nuclear
Yes - present in

SUBUNIT

Nucleus,
import

localization signal
E12.5LV,

Cytoplasm

E14.5LV,

E17.5LV

3
3
P23528
COFILIN-1
18371
Intracellular -
Cell structure -
Widely
Controls actin polymerization and
Yes - present in

Nucleus,
cytoskeleton
distributed in
depolymerization. A major
E12.5LV,

Cytoplasm

various tissues
component of intranuclear and
E14.5LV,

cytoplasmic actin rods.
E17.5LV

3
3
P29966
MYRISTOYLATED
31423
Intracellular
Actin binding, actin
Ubiquitous
MARCKS is the most prominent

ALANINE-RICH C-KINASE

cytoskeleton, cell

cellular substrate for protein kinase C.

SUBSTRATE

structure

MARCKS is a filamentous (F) actin

cross-linking protein.

3
3
Q5JRR8
UBIQUITIN-ACTIVATING
117849
Intracellular
Protein modification
Ubiquitous
Involved with ubiquitin conjugation by
Yes - all rat

ENZYME E1

activating ubiquitin
samples

3
3
P09874
POLY [ADP-RIBOSE]
112953
Nucleus
DNA repair
Ubiquitous
Involved in the base excision repair
Yes - present in

POLYMERASE 1

pathway
E12.5LV,

E17.5LV

3
3
P55072
TRANSITIONAL
89191
Intracellular -
Protein targeting
Ubiquitous
Necessary for the fragmentation of
Yes - all rat

ENDOPLASMIC

Nucleus,
and localization,

Golgi stacks during mitosis and for
samples

RETICULUM ATPASE

Cytoplasm
intracellular protein

their reassembly after mitosis.

traffic

Involved in the formation of the

transitional endoplasmic reticulum.

3
3
Q9NZ08
TYPE 1 TUMOR
107841
Secreted
Protease
Ubiquitous
May play a role in the inactivation of

NECROSIS FACTOR

peptide hormones. May be involved in

RECEPTOR SHEDDING

the regulation of blood pressure.

AMINOPEPTIDASE

REGULATOR ISOFORM A

3
3
P00736
COMPLEMENT C1R
80174
Secreted
Protease -
Plasma
Classical pathway of the complement

SUBCOMPONENT

complement

system

PRECURSOR

mediated immunity

3
3
Q7Z7M0
ISOFORM 1 OF
254573
Membrane
Cell adhesion, cell
Widely
Also known as Multiple EGF like

MULTIPLE EPIDERMAL

structure
distributed
domain protein 4

GROWTH FACTOR-LIKE

DOMAINS 8

3
3
P04406
GLYCERALDEHYDE-3-
35922
Cytoplasm
Glycolysis,
Ubiquitous

Yes - present in

PHOSPHATE

carbohydrate

E12.5LV,

DEHYDROGENASE

degradation

E17.5LV

3
3
P22626
ISOFORM B1 OF
37430
Nucleus
mRNA processing
Ubiquitous
Forms ribonucleosome complexes
Yes - present in

HETEROGENEOUS

E14.5LV

NUCLEAR

RIBONUCLEOPROTEINS

A2/B1

3
3
P10909
CLUSTERIN
52495
Secreted
Function unclear;
Ubiquitous
Seems to be able to bind to cells,
Yes - present in

PRECURSOR

has been implicated

membranes and hydrophobic
E14.5, E17.5LV

in apoptosis

proteins. Has been implicated in

membrane lipid recycling, in apoptotic

cell death, and as a stress-induced

secreted chaperone protein.

3
4
Q86XC7
127 KDA PROTEIN - RAN
126522
Intracellular -
Nuclear-cytoplasmic
Ubiquitous
Involved in the import of proteins with
Yes - one

binding protein 5

Nucleus,
transport

the nuclear localization signal.
isoform found in

Cytoplasm

E12.5LV,

E14.5LV,

E17.5LV, 2nd

isoform found in

E12.5LV, E14.5

4thV, and

E17.5LV

3
4
Q14568
HEAT SHOCK PROTEIN
35674
Cytoplasm
Molecular
Ubiquitous
Heat shock protein family
Yes - all rat

86 (FRAGMENT)

chaperone, protein

samples

folding, stress

response

3
5
P02765
ALPHA-2-HS-
39325
Secreted,
Cysteine protease
Synthesized in
Extracellular matrix glycoprotein
Yes - present in

GLYCOPROTEIN

extracellular
inhibitor
liver and
involved in skeletal development
E14.5, E17.5LV

PRECURSOR

space,

concentrated in

extracellular

bone. Secreted

matrix

in plasma.

3
3
Q14403
GAMMA-G GLOBIN
16969
Intracellular
Oxygen Transport,
Red blood cells
Belongs to the globin family

(FRAGMENT)

Transfer - Carrier

Protein

3
3
P00734
PROTHROMBIN
70037
Secreted
Blood clotting
Expressed by the
Thrombin, converts fibrinogen to fibrin
Yes - present in

PRECURSOR

liver and
and activates factors V, VII, VIII, XIII,
E14.5 4thV,

(FRAGMENT)

secreted in
and, in complex with thrombomodulin.
E17.5LV

plasma

3
3
Q16706
ALPHA-MANNOSIDASE 2
131084
Golgi apparatus
Glycosidase
Ubiquitous
Involved in N-glycosylation
Yes - all rat

samples

3
3
Q9Y6N7
ISOFORM 1 OF
180930
Membrane
Axon guidance
Widely
Receptor for SLIT1 and SLIT2 which
Yes - present in

ROUNDABOUT

receptor
expressed
are thought to act as molecular
E14.5 4thV

HOMOLOG 1

guidance cue in cellular migration,

PRECURSOR

including axonal navigation at the

ventral midline of the neural tube and

projection of axons to different

regions during neuronal development.

3
3
Q15063
ISOFORM 1 OF
93314
Secreted,
Cell adhesion, cell
Widely
Binds to heparin. Induces cell
Yes - present in

PERIOSTIN PRECURSOR

extracellular
structure, cell
expressed with
attachment and spreading and plays a
E14.5

space,
motility
highest levels in
role in cell adhesion

extracellular

aorta, stomach,

matrix

lower

gastrointestinal

tract, placenta,

uterus and

breast.

3
3
P22792
CARBOXYPEPTIDASE N
60615
Secreted
Enzyme regulator,
Plasma
Binds to catalytic subunit, stabilizes it

SUBUNIT 2 PRECURSOR

protein stabilization

and keeps it in circulation

3
3
P55786
PUROMYCIN-SENSITIVE
103276
Intracellular -
Protease
Widely
Involved in proteolytic events
Yes - all rat

AMINOPEPTIDASE

Nucleus,

distributed
essential for cell growth and viability.
samples

Cytoplasm

May act as regulator of neuropeptide

activity.

3
3
P62736
ACTIN, AORTIC SMOOTH
42009
Cytoplasm
Cell motility
Widely
Actins are highly conserved proteins

MUSCLE

distributed
that are involved in various types of

cell motility and are ubiquitously

expressed in all eukaryotic cells

3
3
P02452
COLLAGEN ALPHA-1(I)
138883
Secreted,
Cell adhesion - Cell
Forms the fibrils
Component of connective tissue
Yes - all rat

CHAIN PRECURSOR

extracellular
structure
of tendon,

samples

space,

ligaments and

extracellular

bones

matrix

3
3
Q16819
MEPRIN A SUBUNIT
84368
Membrane
Protease
Widely
Cell surface endopeptidase

ALPHA PRECURSOR

distributed,

highly expressed

in gut

3
13
P23471
PROTEIN TYROSINE
254587
Membrane
Transmembrane
Specifically
May be involved in the regulation of

PHOSPHATASE,

receptor protein
expressed in the
specific developmental processes in

RECEPTOR-TYPE, ZETA1

central nervous
the CNS

PRECURSOR

system

3
4
O95373
120 KDA PROTEIN -
119702
Intracellular -
Nuclear-cytoplasmic
Ubiquitous
Involved in the import of proteins with
Yes - present in

Importin 7

Nucleus,
transport

the nuclear localization signal.
E12.5LV,

Cytoplasm

E17.5LV

3
3
Q9HBB3
DNA-BINDING PROTEIN
32891
Intracellular
Protein biosynthesis -
Ubiquitous

TAXREB107

Ribonucleoprotein

3
3
Q13263
TRIPARTITE MOTIF-
88550
Nucleus
Transcriptional
Ubiquitous
Acts as a transcriptional corepressor
Yes - present in

CONTAINING 28

Regulation

E12.5LV,

PROTEIN

E14.5LV

3
3
P51693
AMYLOID-LIKE PROTEIN
72176
Cell membrane,
May play a role in
Cerebral cortex
Can regulate neurite outgrowth
Yes - present in

1 PRECURSOR

Cytoplasm
postsynaptic

through binding to components of the
E14.5 4thV,

function

extracellular matrix.
E17.5LV

3
4
P20849
ISOFORM 1 OF
91855
Secreted,
Extracellular matrix
Structural
Component of connective tissue

COLLAGEN ALPHA-1(IX)

extracellular
structural
component of

CHAIN PRECURSOR

space,
constituent
hyaline cartilage

extracellular

and vitreous of

matrix

the eye

3
3
Q14112
NIDOGEN-2 PRECURSOR
151395
Secreted,
Cell adhesion
Heart, placenta
Cell adhesion glycoprotein which is
Yes - all rat

extracellular

and bone
widely distributed in basement
samples

space,

membranes

extracellular

matrix

3
3
Q6EMK4
VASORIN PRECURSOR
71713
Membrane
Inhibitor of TGF-
Aorta, kidney,
Modulates the arterial response to

beta signaling
placenta, brain,
injury

heart, liver, lung

and skeletal

muscle.

3
3
P11142
ISOFORM 1 OF HEAT
70898
Cytoplasm
Chaperone
Ubiquitous
Belongs to the heat shock protein 70
Yes - present in

SHOCK COGNATE 71 KDA

family
E12.5LV,

PROTEIN

E14.5LV,

E17.5LV

3
3
Q92823
ISOFORM 1 OF
144074
Membrane
Cell adhesion,
Widely
Belongs to the immunoglobulin super
Yes - all rat

NEURONAL CELL

involved in neuron-
distributed,
family
samples

ADHESION MOLECULE

neuron adhesion
highly expressed

PRECURSOR

in brain

2
2
P30086
PHOSPHATIDYLETHANOLAMINE-
20926
Cytoplasm
Serine Protease
Ubiquitous
Serine protease inhibitor which
Yes - present in

BINDING

Inhibitor

inhibits thrombin, neuropsin and
E17.5LV

PROTEIN 1

chymotrypsin.

2
4
Q6PIH6
IGKV1-5 (Immunoglobulin
26234
Membrane
MHC class I protein
Unknown
Antigen processing and presentation

kappa variable 1-5)

complex

of peptides

PROTEIN

2
2
P08697
ALPHA-2-ANTIPLASMIN
55064
Secreted
Serine Protease
Plasma
Belongs to the serpin family
Yes - all rat

PRECURSOR

Inhibitor - Blood

samples

coagulation

2
2
Q02487
ISOFORM 2A OF
99962
Membrane
Cell adhesion
Expressed in
Component of intercellular

DESMOCOLLIN-2

epithelia,
desmosome junctions.

PRECURSOR

myocardium and

lymph nodes

2
2
P49736
DNA REPLICATION
101896
Nucleus
DNA binding, cell
Ubiquitous
Acts as a factor that allows the DNA
Yes - present in

LICENSING FACTOR

cycle progression

to undergo a single round of
E12.5LV,

MCM2

replication per cell cycle. Required for
E17.5LV

the entry in S phase and for cell

division

2
2
P26022
PENTRAXIN-RELATED
42020
Secreted
Inflammatory
Widely
Plays a role in the regulation of innate

PROTEIN PTX3

response
distributed,
resistance to pathogens, and

PRECURSOR

highly expressed
inflammatory reactions.

in connective

tissue

2
2
O60462
ISOFORM A22 OF
104831
Membrane
Vascular endothelial
Widely
High affinity receptor for semaphorins
Yes - present in

NEUROPILIN-2

growth factor
distributed
3C, 3F, VEGF-165 and VEGF-145
E14.5 4thV

PRECURSOR

receptor activity -

isoforms of VEGF, and the PLGF-2

involved in axon

isoform of PGF

guidance

2
4
P51884
LUMICAN PRECURSOR
38429
Secreted,
ECM structural
Widely
Binds to laminin
Yes - all rat

extracellular
component
distributed,

samples

space,

highly expressed

extracellular

in cornea

matrix

2
2
P01042
ISOFORM HMW OF
71945
Secreted,
Cysteine protease
Plasma
Bradykinin is released from kininogen
Yes - all rat

KININOGEN-1

extracellular
inhibitor

by plasma kallikrein. Plays an
samples

PRECURSOR

space,

important role in blood coagulation.

extracellular

matrix

2
2
P55060
ISOFORM 1 OF
110417
Intracellular -
Nuclear-cytoplasmic
Ubiquitous
Export receptor for importin-alpha.

EXPORTIN-2

Nucleus,
shuttling

Cytoplasm

2
2
P06748
ISOFORM 2 OF
29465
Nucleus,
rRNA metabolism
Ubiquitous
Associated with nucleolar

NUCLEOPHOSMIN

nucleolus

ribonucleoprotein structures and bind

single-stranded nucleic acids. It may

function in the assembly and/or

transport of ribosome

2
2
Q5UE57
ALCADEIN BETA
107033
Membrane
Cell adhesion
Widely
Calcium ion binding

distributed,

expressed highly

in brain

2
2
P27348
14-3-3 PROTEIN THETA
27764
Cytoplasm
Chaperone, signal
Widely
Adapter protein implicated in the
Yes - present in

transduction
distributed,
regulation of a large spectrum of both
E12.5LV,

expressed highly
general and specialized signaling
E14.5LV,

in brain, heart
pathways.
E17.5LV

and pancreas

2
2
P36578
60S RIBOSOMAL
47566
Cytoplasm
Protein biosynthesis
Ubiqutous
Cytosolic large ribosomal subunit
Yes - present in

PROTEIN L4

E12.5LV,

E14.5LV

2
2
P08253
72 KDA TYPE IV
73882
Secreted,
Hydrolase,
Fibroblasts
Cleaves gelatin and collagen

COLLAGENASE

extracellular
proteolysis

PRECURSOR

space,

extracellular

matrix

2
2

HYPOTHETICAL
42003
Unknown
Unknown
Unknown
Unknown

PROTEIN LOC345651

2
2
P08572
COLLAGEN ALPHA-2(IV)
167535
Secreted,
Extracellular matrix
Widely
Type IV collagen is the major
Yes - present in

CHAIN PRECURSOR

extracellular
structural
distributed
structural component of glomerular
E12.5LV, E14.5

space,
constituent

basement membranes, forming a

extracellular

meshwork together with laminins,

matrix

proteoglycans and entactin/nidogen.

2
2
P52701
ISOFORM GTBP-ALT OF
120563
Nucleus
DNA mismatch
Ubiquitous
Restores repair of base-base and

DNA MISMATCH REPAIR

repair

single-nucleotide insertion-deletion

PROTEIN MSH6

mismatches.

2
2
P14625
ENDOPLASMIN
92469
Endoplasmic
Chaperone, protein
Widely
Molecular chaperone that functions in
Yes - all rat

PRECURSOR

reticulum
folding
distributed
the processing and transport of
samples

secreted proteins
(TUMOR

REJECTION

ANTIGEN

GP96)

2
2
P05067
ISOFORM APP770 OF
86943
Membrane.
Cell surface
Expressed in all
During neuronal differentiation, the
Yes - present in

AMYLOID BETA A4

After alpha-
receptor involved in
fetal tissues
Thr-743 phosphorylated form is
E12.5LV, E14.5

PROTEIN PRECURSOR

secretase
neurite growth,
examined with
located mainly in growth cones,
4thV, E17.5LV

(FRAGMENT)

cleavage,
neuronal adhesion
highest levels in
moderately in neurites and sparingly

soluble APP is
and axonogenesis.
brain, kidney,
in the cell body. Defects in APP are a

released into

heart and
cause of autosomal dominant

the extracellular

spleen.
Alzheimer disease.

space and the

C-terminal is

internalized to

endosomes and

lysosomes.

2
2
P09651
HETEROGENEOUS
38747
Intracellular -
Component of
Ubiquitous
Involved in the packaging of pre-
Yes - present in

NUCLEAR

Nucleus,
ribonucleosomes.

mRNA into hnRNP particles, transport
E12.5LV

RIBONUCLEOPROTEIN

Cytoplasm
Nuclear mRNA

of poly(A) mRNA from the nucleus to

A1 ISOFORM B

splicing

the cytoplasm and may modulate

splice site selection.

2
2
P61978
ISOFORM 1 OF
50976
Intracellular -
RNA processing
Ubiquitous
One of the major pre-mRNA-binding

HETEROGENEOUS

Nucleus,

proteins. Binds tenaciously to poly(C)

NUCLEAR

Cytoplasm

sequences.

RIBONUCLEOPROTEIN K

2
2
P84077
ADP-RIBOSYLATION
20566
Membrane -
Protein trafficking
Ubiquitous
Modulates vesicle budding and

FACTOR 1

Golgi

uncoating within the Golgi complex.

2
2
P01034
CYSTATIN C
15799
Secreted,
Cysteine protease
Widely
Important regulator of cysteine
Yes - all rat

PRECURSOR

extracellular
inhibitor
distributed,
proteases in a number of physiologic
samples

space,

highly expressed
functions

extracellular

in epididymis,

matrix

vas deferens,

brain, thymus,

and ovary

2
2
P68371
TUBULIN BETA-2C CHAIN
49831
Intracellular
Cell structure, cell
Widely
Tubulin is the major constituent of
Yes - all rat

mobility,
distributed
microtubules
samples

chromosome

segregation,

intracellular protein

traffic

2
2
P08123
COLLAGEN ALPHA-2(I)
129412
Secreted,
Cell adhesion - Cell
Forms the fibrils
Involved in skeletal development
Yes - all rat

CHAIN PRECURSOR

extracellular
structure
of tendon,

samples

space,

ligaments and

extracellular

bones

matrix

2
4
P01842
IGLC1 PROTEIN
24793
Membrane
Immune response
Ubiquitous
MHC class I protein complex antigen

antigen binding

processing and presentation.

2
2
P62424
60S RIBOSOMAL
29864
Cytoplasm
Protein biosynthesis
Ubiquitous
Cytosolic large ribosomal subunit
Yes - present in

PROTEIN L7A

E12.5LV,

E17.5LV

2
2
P24043
LAMININ ALPHA 2
343905
Secreted,
Extracellular matrix
Placenta,
Laminin is thought to mediate the

SUBUNIT PRECURSOR

extracellular
linker protein-
striated muscle,
attachment, migration and

space,
mediated signaling
peripheral nerve,
organization of cells into tissues

extracellular

cardiac muscle,
during embryonic development by

matrix

pancreas, lung,
interacting with other extracellular

spleen, kidney,
matrix components.

adrenal gland,

skin, testis,

meninges,

choroid plexus,

and some other

regions of the

brain

2
3
P04196
HISTIDINE-RICH
59578
Secreted
Function unknown
Expressed by the
It binds heme, dyes and divalent
Yes - present in

GLYCOPROTEIN

liver and
metal ions. It can inhibit rosette
E12.5LV,

PRECURSOR

secreted in
formation and is known to interact
E14.5LV

plasma
with heparin, thrombospondin, and

the lysine-binding site of plasminogen.

On the basis of its homology with

HMW kininogen, the His-rich region of

this protein may mediate the contact

activation phase of intrinsic blood

coagulation cascade.

2
2
P62826
GTP-BINDING NUCLEAR
24292
Intracellular -
Nuclear-cytoplasmic
Ubiquitous
Required for the import of protein into
Yes - all rat

PROTEIN RAN

Nucleus,
shuttling

the nucleus and also for RNA export.
samples

Cytoplasm

2
2
P69905
HEMOGLOBIN SUBUNIT
15126
Intracellular
Oxygen Transport,
Red blood cells
Involved in oxygen transport from the
Yes - present in

ALPHA

Transfer - Carrier

lung to the various peripheral tissues
E12.5LV,

Protein

E17.5LV

2
2
P68871
HEMOGLOBIN SUBUNIT
15867
Intracellular
Oxygen Transport,
Red blood cells
Involved in oxygen transport from the
Yes - present in

BETA

Transfer - Carrier

lung to the various peripheral tissues
E17.5LV

Protein

2
2
P08603
ISOFORM 1 OF
139070
Secreted
Complement
Expressed by the
Involved in the regulation of
Yes - all rat

COMPLEMENT FACTOR

activation
liver and
complement activation
samples

H PRECURSOR

secreted in

plasma

2
2
P53618
COATOMER SUBUNIT
107139
Cytoplasm.
Vesicle mediated
Ubiquitous
Coatomer complex is required for
Yes - present in

BETA

Golgi apparatus
transport

budding from Golgi membranes, and
E12.5LV,

is essential for the retrograde Golgi-
E17.5LV

to-ER transport of dilysine-tagged

proteins.

2
2
P53675
ISOFORM 1 OF
187030
Cytoplasmic
Receptor-mediated
Ubiquitous
Involved in the formation of clathrin
Yes - all rat

CLATHRIN HEAVY CHAIN 2

vesicle;
endocytosis

coated vesicles during vesicle
samples

cytoplasmic

endocytosis

vesicle

membrane

2
2
P09871
COMPLEMENT C1S
76684
Secreted
Serine protease
Plasma
Immune response - Complement
Yes - present in

SUBCOMPONENT

activation
E17.5LV

PRECURSOR

2
2
Q8NBJ4
GOLGI
46273
Golgi apparatus
Function unknown
Widely
Cellular response protein to viral

PHOSPHOPROTEIN 2

expressed
infection

2
2
P13639
ELONGATION FACTOR 2
95207
Cytoplasm
Protein biosynthesis
Ubiquitous
Promotes translocation of protein
Yes - all rat

chain from A site to P site on
samples

ribosome

2
2
P68366
TUBULIN ALPHA-1 CHAIN
49924
Intracellular
Cell structure, cell
Widely
Tubulin is the major constituent of
Yes - all rat

mobility, intracellular
distributed
microtubules
samples

protein traffic

2
2
Unknown
22 KDA PROTEIN
22066
Unknown
Unknown
Unknown
Unknown

2
2
P45974
ISOFORM LONG OF
95786
Intracellular -
Protease
Ubiquitous
Cleaves linear and branched
Yes - present in

UBIQUITIN CARBOXYL-

Lysosome

multiubiquitin polymers with a marked
E14.5LV,

TERMINAL HYDROLASE 5

preference for branched polymers.
E17.5LV

2
2
Q14008
CYTOSKELETON-
225509
Intracellular -
Centrosome
Skeletal muscle,
Plays a major role in organizing

ASSOCIATED PROTEIN 5

centrosome
organization and
brain, heart,
spindle poles

biogenesis, and
placenta, lung,

establishment and
liver, kidney and

maintenance of
pancreas

microtubule

cytoskeleton polarity

2
7
Q8IUK7
ALB PROTEIN
45160
Secreted
Transport,
Plasma
Serum albumin, the main protein of
Yes - all rat

Transfer/Carrier

plasma, has a good binding capacity
samples

for water, Ca(2+), Na(+), K(+), fatty

acids, hormones, bilirubin and drugs.

Its main function is the regulation of

the colloidal osmotic pressure of

blood.

2
3
P55285
ISOFORM 1 OF
88309
Membrane
Cell adhesion
Highly expressed
Also known as Kidney cadherin
Yes - all rat

CADHERIN-6

in ovary, brain,

samples

PRECURSOR

cerebellum, and

kidney

2
2
P23246
ISOFORM LONG OF
76149
Nucleus
mRNA processing
Ubiquitous
DNA- and RNA binding protein,

SPLICING FACTOR,

involved in several nuclear processes.

PROLINE- AND

Essential pre-mRNA splicing factor

GLUTAMINE-RICH

required early in spliceosome

formation.

2
2
P04275
VON WILLEBRAND
309299
Secreted
Cell adhesion -
Plasma
Important in the maintenance of
Yes - all rat

FACTOR PRECURSOR

Blood clotting

hemostasis, it promotes adhesion of
samples

platelets to the sites of vascular injury.

Also acts as a chaperone for

coagulation factor VIII, delivering it to

the site of injury, stabilizing its

heterodimeric structure and protecting

it from premature clearance from

plasma.

2
3
P01011
ISOFORM 1 OF ALPHA-1-
47651
Secreted
Serine protease
Plasma
Belongs to the serpin family

ANTICHYMOTRYPSIN

inhibitor

PRECURSOR

2
2
P09211
GLUTATHIONE S-
23225
Cytoplasm
Transferase
Ubiquitous
Involved in detoxifing compounds by
Yes - present in

TRANSFERASE P

linking glutathione to hydrophobic
E12.5LV

substances

2
2
P33991
DNA REPLICATION
96558
Nucleus
Involved in the
Ubiquitous
Involved in chromatin binding

LICENSING FACTOR

control of DNA

MCM4

replication

2
8
P23142
ISOFORM C OF FIBULIN-
74462
Secreted,
Cell adhesion
Widely
Involved in a number of various ECM
Yes - all rat

1 PRECURSOR

extracellular
mediated signaling
expressed during
related functions during development
samples

space,

embryonic
including cell adhesion, migration,

extracellular

development
and ecm architecture. Binds to laminin

matrix

and nidogen (both found in the CSF).

2
3
Q60847
ISOFORM LONG OF
333194
Secreted,
Cell adhesion - Cell
Widely
Component of connective tissue

COLLAGEN ALPHA-1(XII)

extracellular
structure
distributed

CHAIN PRECURSOR

space,

extracellular

matrix

2
3
Q24JP5
TRANSMEMBRANE
110110
Membrane
Integral to
Unknown
Unknown

PROTEIN 132A ISOFORM B

membrane

2
2
Q16610
EXTRACELLULAR
60674
Secreted,
Signal transduction
Widely
Positive regulation of I-kappaB

MATRIX PROTEIN 1

extracellular
activity
distributed
kinase/NF-kappaB cascade

PRECURSOR

space,

extracellular

matrix

2
2
P13671
COMPLEMENT
105752
Secreted
Immune response -
Plasma
Involved in the formation of the lytic
Yes - present in

COMPONENT C6

complement

complex, which inserts into plasma
E17.5LV

PRECURSOR

pathway

membranes and causes cells to lyse.

2
2
P10586
RECEPTOR-TYPE
211845
Membrane
Transmembrane
Widely
Signaling molecule invovled in cell
Yes-LAR

TYROSINE-PROTEIN

receptor protein
distributed
growth and differentiation
RECEPTOR-

PHOSPHATASE F

involved in cell

LINKED

PRECURSOR

adhesion

TYROSINE

PHOSPHATASE

present in all rat

samples

2
2
Q9HCU4
CADHERIN EGF LAG
317453
Membrane
G-protein coupled
Highest
May have an important role in cell/cell
Yes - present in

SEVEN-PASS G-TYPE

receptor protein
expression in
signaling during nervous system
E12.5LV,

RECEPTOR 2

signaling pathway
brain and testis
formation
E14.5LV

PRECURSOR

2
2
P62917
60S RIBOSOMAL
27893
Cytoplasm
Protein Biosynthesis
Ubiquitous
Ribosomal structural protein
Yes - present in

PROTEIN L8

E12.5LV, E14.5

4thV

2
2
P33176
KINESIN HEAVY CHAIN
109685
Intracellular
Microtubule motor
Ubiquitous
Microtubute-dependent motor
Yes - present in

activity

required for normal distribution of
E17.5LV

mitochondria and lysosomes

2
2
P62258
14-3-3 PROTEIN EPSILON
29174
Cytoplasm
Chaperone, signal
Ubiquitous
Adapter protein implicated in the
Yes - all rat

transduction -

regulation of a large spectrum of both
samples

intracellular

general and specialized signaling

signaling cascade

pathway

2
4
Q8NF17
FLJ00385 PROTEIN
56111
Membrane
Unknown
Unknown
IgG like protein

(FRAGMENT)

2
2
P43243
MATRIN-3
94623
Nucleus
Function unclear
Ubiquitous
May play a role in trascription, nuclear
Yes - present in

structure, or nuclear retention of
E12.5LV,

defective RNAs
E17.5LV

2
2
P15924
ISOFORM DPI OF
331776
Membrane
Cell adhesion, cell
Isoform DPI is a
Major high molecular weight protein of
Yes - present in

DESMOPLAKIN

junctions
constituent of all
desmosomes
E12.5LV,

desmosomes

E17.5LV

2
2
P19652
ALPHA-1-ACID
23603
Secreted
Immune response -
Plasma
Appears to function in modulating the

GLYCOPROTEIN 2

acute phase

activity of the immune system during

PRECURSOR

the acute-phase reaction

2
2
P60842
EUKARYOTIC INITIATION
46154
Intracellular
Protein biosynthesis
Ubiquitous
Required for mRNA binding to

FACTOR 4A-I

ribosome

2
2
P00338
LACTATE
36689
Cytoplasm
Anaerobic glycolysis
Widely
Catalyzes the conversion of lactate
Yes - present in

DEHYDROGENASE A

distributed,
and NAD to pyruvate and NADH
E12.5LV, E14.5

highly expressed

in muscle tissue

2
2
Q6UX71
ISOFORM 1 OF PLEXIN
59583
Membrane
Cell surface
Endothelial cells
May play a role in tumor angiogenesis

DOMAIN-CONTAINING

endothelial marker

PROTEIN 2 PRECURSOR

2
2
P30041
PEROXIREDOXIN-6
24904
Cytoplasm
Oxidoreductase,
Ubiquitous
Involved in redox regulation of the cell

Peroxidase

in response to oxidative stress.

2
2
O14594
NEUROCAN CORE
142973
Secreted,
Extracellular matrix
Brain
May modulate neuronal adhesion and
Yes - present in

PROTEIN PRECURSOR

extracellular
protein-mediated

neurite growth during development by
E14.5 4thV,

space,
signaling, cell

binding to neural cell adhesion
E17.5LV

extracellular
adhesion, cell

molecules.

matrix
motility

2
2
Q12860
ISOFORM 1 OF
113320
Membrane
Cell adhesion
Widely
Mediates cell surface interactions
Yes - present in

CONTACTIN-1

distributed,
during nervous system development.
E17.5LV

PRECURSOR

highly expressed
Involved in the formation of axon-glial

in brain
junctions in myelinated peripheral

nerves and in the signaling between

axons and myelinating glial cells.

Participates in oligodendrocytes

generation by acting as a ligand of

NOTCH1.

2
2
Q8TF66
LEUCINE-RICH REPEAT-
64396
Membrane
Unknown
Brain and
Unknown

CONTAINING PROTEIN

placenta

15 PRECURSOR

TABLE 2

Protein list from mass spectrometry CS19 human CSF.

Number

of unique
Total

peptides
number

from
of

protein
peptides
Protein matches for CS19 human CSF

194
571

Homo
sapiens (Human) APOLIPOPROTEIN B-100 PRECURSOR. [MASS = 515563

120
176

Homo
sapiens (Human) DYNEIN HEAVY CHAIN, CYTOSOLIC. [MASS = 532408

68
168

Homo
sapiens (Human) MICROTUBULE-ASSOCIATED PROTEIN 1B. [MASS = 270620

53
58

Homo
sapiens (Human) MYOSIN-10. [MASS = 228939

52
66

Homo
sapiens (Human) FILAMIN A, ALPHA. [MASS = 280018

48
54

Homo
sapiens (Human) ISOFORM 1 OF SPECTRIN ALPHA CHAIN, BRAIN. [MASS = 284539

39
72

Homo
sapiens (Human) FATTY ACID SYNTHASE. [MASS = 273400

36
73

Homo
sapiens (Human) VIMENTIN. [MASS = 53520

36
36

Homo
sapiens (Human) ISOFORM LONG OF SPECTRIN BETA CHAIN, BRAIN 1. [MASS = 274631

36
72

Homo
sapiens (Human) NESTIN. [MASS = 176706

35
172

Homo
sapiens (Human) ALB PROTEIN. [MASS = 71705

34
127

Homo
sapiens (Human) SEROTRANSFERRIN PRECURSOR. [MASS = 77050

31
41

Homo
sapiens (Human) Heat shock 70 kDa protein 5. [MASS = 72422

31
45

Homo
sapiens (Human) ISOFORM 1 OF FIBRONECTIN PRECURSOR. [MASS = 262607

30
36

Homo
sapiens (Human) MOESIN. [MASS = 67689

29
84

Homo
sapiens (Human) HYPOTHETICAL PROTEIN DKFZP761K0511. [MASS = 84843

28
58

Homo
sapiens (Human) ISOFORM 1 OF HEAT SHOCK COGNATE 71 KDA PROTEIN. [MASS = 70898

27
46

Homo
sapiens (Human) ELONGATION FACTOR 2. [MASS = 95207

27
30

Homo
sapiens (Human) PRE-MRNA-PROCESSING-SPLICING FACTOR 8. [MASS = 273600

26
44

Homo
sapiens (Human) APOLIPOPROTEIN A-IV PRECURSOR. [MASS = 45399

26
102

Homo
sapiens (Human) GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE. [MASS = 35922

26
29

Homo
sapiens (Human) ISOFORM 1 OF DNA-DEPENDENT PROTEIN KINASE CATALYTIC

SUBUNIT. [MASS = 469089

25
109

Homo
sapiens (Human) TUBULIN BETA-2C CHAIN. [MASS = 49831

25
41

Homo
sapiens (Human) CLATHRIN HEAVY CHAIN 1. [MASS = 191615

24
27

Homo
sapiens (Human) MYOSIN-9. [MASS = 226401

23
30

Homo
sapiens (Human) T-COMPLEX PROTEIN 1 SUBUNIT BETA. [MASS = 57357

23
31

Homo
sapiens (Human) ENDOPLASMIN PRECURSOR. [MASS = 92469

23
35

Homo
sapiens (Human) ISOFORM 1 OF CULLIN-ASSOCIATED NEDD8-DISSOCIATED PROTEIN 1.

[MASS = 136376

23
23

Homo
sapiens (Human) ISOFORM 2 OF MICROTUBULE-ACTIN CROSSLINKING FACTOR 1,

ISOFORMS 1/2/3/5. [MASS = 620418

23
28

Homo
sapiens (Human) ISOFORM 2 OF HECT, UBA AND WWE DOMAIN-CONTAINING

PROTEIN 1. [MASS = 480198

22
77

Homo
sapiens (Human) CREATINE KINASE B-TYPE. [MASS = 42644

22
26

Homo
sapiens (Human) KINESIN HEAVY CHAIN. [MASS = 109685

22
34

Homo
sapiens (Human) IMPORTIN BETA-1 SUBUNIT. [MASS = 97170

22
31

Homo
sapiens (Human) DNA REPLICATION LICENSING FACTOR MCM2. [MASS = 101896

22
24

Homo
sapiens (Human) STRUCTURAL MAINTENANCE OF CHROMOSOME 3. [MASS = 141542

22
42

Homo
sapiens (Human) APOLIPOPROTEIN A-I PRECURSOR. [MASS = 30778

21
44

Homo
sapiens (Human) ISOFORM M1 OF PYRUVATE KINASE ISOZYMES M1/M2. [MASS = 57931

21
35

Homo
sapiens (Human) DPYSL3 PROTEIN. [MASS = 73910

21
34

Homo
sapiens (Human) RAN BINDING PROTEIN 5. [MASS = 125545

21
35

Homo
sapiens (Human) TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE. [MASS = 89191

21
167

Homo
sapiens (Human) ALPHA-FETOPROTEIN PRECURSOR. [MASS = 68678

20
26

Homo
sapiens (Human) DIHYDROPYRIMIDINASE-RELATED PROTEIN 1. [MASS = 62184

20
27

Homo
sapiens (Human) ATP-DEPENDENT RNA HELICASE A. [MASS = 140881

20
30

Homo
sapiens (Human) MATRIN-3. [MASS = 94623

19
35

Homo
sapiens (Human) RAB GDP DISSOCIATION INHIBITOR ALPHA [MASS = 50583

19
24

Homo
sapiens (Human) APOLIPOPROTEIN E PRECURSOR. [MASS = 36154

19
20

Homo
sapiens (Human) C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC. [MASS = 101428

19
29

Homo
sapiens (Human) ISOFORM LONG OF SPLICING FACTOR, PROLINE- AND GLUTAMINE-RICH.

[MASS = 76149

19
35

Homo
sapiens (Human) ATP-DEPENDENT DNA HELICASE 2 SUBUNIT 2. [MASS = 82573

19
23

Homo
sapiens (Human) ISOFORM 1 OF MICROTUBULE-ASSOCIATED PROTEIN 2. [MASS = 199539

19
22

Homo
sapiens (Human) PROTEIN DISULFIDE-ISOMERASE A4 PRECURSOR. [MASS = 72932

18
38

Homo
sapiens (Human) FIBRINOGEN BETA CHAIN PRECURSOR. [MASS = 55928

18
23

Homo
sapiens (Human) PHOSPHOGLYCERATE KINASE 1. [MASS = 44483

18
18

Homo
sapiens (Human) DNA REPLICATION LICENSING FACTOR MCM6. [MASS = 92889

18
37

Homo
sapiens (Human) ISOFORM 1 OF NUCLEAR AUTOANTIGENIC SPERM PROTEIN.

[MASS = 85238

18
38

Homo
sapiens (Human) TRIPARTITE MOTIF-CONTAINING 28 PROTEIN. [MASS = 88550

18
20

Homo
sapiens (Human) ISOFORM 1 OF GENERAL TRANSCRIPTION FACTOR II-I. [MASS = 112416

18
35

Homo
sapiens (Human) NCL PROTEIN. [MASS = 51641

18
31

Homo
sapiens (Human) INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H2 PRECURSOR.

[MASS = 106436

18
29

Homo
sapiens (Human) ISOFORM 5 OF INTERLEUKIN ENHANCER-BINDING FACTOR 3.

[MASS = 74607

17
30

Homo
sapiens (Human) HEAT SHOCK 70 KDA PROTEIN 4. [MASS = 94300

17
19

Homo
sapiens (Human) LUPUS LA PROTEIN. [MASS = 46837

17
89

Homo
sapiens (Human) TUBULIN ALPHA-1 CHAIN. [MASS = 49924

17
24

Homo
sapiens (Human) POLY [ADP-RIBOSE] POLYMERASE 1. [MASS = 112953

17
18

Homo
sapiens (Human) LEUCYL-TRNA SYNTHETASE, CYTOPLASMIC. [MASS = 134466

17
21

Homo
sapiens (Human) PUROMYCIN-SENSITIVE AMINOPEPTIDASE. [MASS = 103276

17
17

Homo
sapiens (Human) ISOFORM 1 OF STRUCTURAL MAINTENANCE OF CHROMOSOME

2-LIKE 1 PROTEIN. [MASS = 135781

16
126

Homo
sapiens (Human) ALPHA-1-ANTITRYPSIN PRECURSOR. [MASS = 46737

16
19

Homo
sapiens (Human) EUKARYOTIC INITIATION FACTOR 4A-I. [MASS = 46154

16
20

Homo
sapiens (Human) T-COMPLEX PROTEIN 1 SUBUNIT ETA. [MASS = 59367

16
21

Homo
sapiens (Human) ISOCITRATE DEHYDROGENASE (NADP) CYTOPLASMIC. [MASS = 46659

16
17

Homo
sapiens (Human) KINESIN HEAVY CHAIN ISOFORM 5C. [MASS = 109495

16
35

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN U. [MASS = 88814

16
18

Homo
sapiens (Human) SPLICING FACTOR 3B SUBUNIT 1. [MASS = 145815

16
18

Homo
sapiens (Human) 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2.

[MASS = 100200

16
17

Homo
sapiens (Human) CHAPERONIN CONTAINING TCP1, SUBUNIT 8. [MASS = 59779

15
17

Homo
sapiens (Human) ATP-CITRATE SYNTHASE. [MASS = 120825

15
53

Homo
sapiens (Human) ISOFORM GAMMA-B OF FIBRINOGEN GAMMA CHAIN

PRECURSOR. [MASS = 51512

15
18

Homo
sapiens (Human) ISOFORM 1 OF EXPORTIN-2. [MASS = 110417

15
22

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN

M ISOFORM A. [MASS = 77516

15
22

Homo
sapiens (Human) ISOFORM 2 OF FAR UPSTREAM ELEMENT-BINDING

PROTEIN 1. [MASS = 68605

15
18

Homo
sapiens (Human) SPLICING FACTOR 3 SUBUNIT 1. [MASS = 88886

15
43

Homo
sapiens (Human) HEAT SHOCK PROTEIN HSP 90-ALPHA 2. [MASS = 98113

15
23

Homo
sapiens (Human) 14-3-3 PROTEIN EPSILON. [MASS = 29174

15
15

Homo
sapiens (Human) ISOFORM 1 OF VINCULIN. [MASS = 116591

15
18

Homo
sapiens (Human) ALANYL-TRNA SYNTHETASE. [MASS = 106801

14
18

Homo
sapiens (Human) DNA REPLICATION LICENSING FACTOR MCM4. [MASS = 96558

14
14

Homo
sapiens (Human) ISOFORM 1 OF SPECTRIN BETA CHAIN, BRAIN 2. [MASS = 271295

14
21

Homo
sapiens (Human) FAR UPSTREAM ELEMENT-BINDING PROTEIN 2. [MASS = 72709

14
14

Homo
sapiens (Human) PROTEIN DISULFIDE-ISOMERASE A3 PRECURSOR. [MASS = 56782

14
19

Homo
sapiens (Human) RAB GDP DISSOCIATION INHIBITOR BETA. [MASS = 50663

14
21

Homo
sapiens (Human) DEAH (ASP-GLU-ALA-HIS) BOX POLYPEPTIDE 15. [MASS = 92829

14
22

Homo
sapiens (Human) LACTATE DEHYDROGENASE A. [MASS = 36689

14
22

Homo
sapiens (Human) EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 10.

[MASS = 166569

14
21

Homo
sapiens (Human) ISOFORM BETA OF HEAT-SHOCK PROTEIN 105 KDA. [MASS = 92116

14
25

Homo
sapiens (Human) ISOFORM 1 OF POLYADENYLATE-BINDING PROTEIN 1.

[MASS = 70671

13
19

Homo
sapiens (Human) 6-PHOSPHOGLUCONATE DEHYDROGENASE,

DECARBOXYLATING. [MASS = 53009

13
30

Homo
sapiens (Human) ISOFORM 1 OF HETEROGENEOUS NUCLEAR

RIBONUCLEOPROTEIN K. [MASS = 50976

13
14

Homo
sapiens (Human) DREBRIN. [MASS = 71294

13
14

Homo
sapiens (Human) EXPORTIN-1. [MASS = 123386

13
13

Homo
sapiens (Human) SMARCA4 ISOFORM 2. [MASS = 188149

13
15

Homo
sapiens (Human) HYDROXYMETHYLGLUTARYL-COA SYNTHASE,

CYTOPLASMIC. [MASS = 57294

13
16

Homo
sapiens (Human) RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE

SUBUNIT. [MASS = 90070

13
28

Homo
sapiens (Human) HNRPA2B1 PROTEIN. [MASS = 28412

13
45

Homo
sapiens (Human) ENOLASE 1. [MASS = 47169

13
15

Homo
sapiens (Human) ALPHA-ACTININ-1. [MASS = 103058

13
29

Homo
sapiens (Human) DIHYDROPYRIMIDINASE-LIKE 2. [MASS = 67017

13
15

Homo
sapiens (Human) DIHYDROPYRIMIDINASE-RELATED PROTEIN 4. [MASS = 61878

13
20

Homo
sapiens (Human) INSULIN-LIKE GROWTH FACTOR 2 MRNA BINDING

PROTEIN 1. [MASS = 63481

13
17

Homo
sapiens (Human) ISOFORM 2 OF NEUTRAL ALPHA-GLUCOSIDASE AB

PRECURSOR. [MASS = 109438

13
13

Homo
sapiens (Human) ISOFORM P150 OF DYNACTIN-1. [MASS = 141695

13
14

Homo
sapiens (Human) SPLICING FACTOR 3B SUBUNIT 2. [MASS = 100228

13
25

Homo
sapiens (Human) HEMOGLOBIN SUBUNIT EPSILON. [MASS = 16072

13
17

Homo
sapiens (Human) DNA REPLICATION LICENSING FACTOR MCM5. [MASS = 82286

13
18

Homo
sapiens (Human) FASCIN. [MASS = 54399

13
15

Homo
sapiens (Human) GLUTAMYL-PROLYL TRNA SYNTHETASE. [MASS = 170591

13
14

Homo
sapiens (Human) DNA REPLICATION LICENSING FACTOR MCM3. [MASS = 90981

12
25

Homo
sapiens (Human) UBIQUITIN-ACTIVATING ENZYME E1. [MASS = 117849

12
68

Homo
sapiens (Human) TRANSTHYRETIN PRECURSOR. [MASS = 15887

12
18

Homo
sapiens (Human) SPLICING FACTOR 3B SUBUNIT 3. [MASS = 135592

12
13

Homo
sapiens (Human) PHOSPHOFRUCTOKINASE, MUSCLE. [MASS = 85183

12
41

Homo
sapiens (Human) TUBULIN BETA-3 CHAIN. [MASS = 50433

12
12

Homo
sapiens (Human) ALPHA-ACTININ-4. [MASS = 104854

12
15

Homo
sapiens (Human) ISOFORM 1 OF GELSOLIN PRECURSOR. [MASS = 85698

12
14

Homo
sapiens (Human) MULTIFUNCTIONAL PROTEIN ADE2. [MASS = 49679

12
15

Homo
sapiens (Human) DIHYDROPYRIMIDINASE-RELATED PROTEIN 5. [MASS = 61421

12
22

Homo
sapiens (Human) ANNEXIN A5. [MASS = 35806

12
25

Homo
sapiens (Human) L-LACTATE DEHYDROGENASE B CHAIN. [MASS = 36507

12
16

Homo
sapiens (Human) CALNEXIN PRECURSOR. [MASS = 67568

12
23

Homo
sapiens (Human) ISOFORM 1 OF FIBRINOGEN ALPHA CHAIN PRECURSOR.

[MASS = 94973

12
18

Homo
sapiens (Human) NON-POU DOMAIN-CONTAINING OCTAMER-BINDING PROTEIN.

[MASS = 54232

12
13

Homo
sapiens (Human) 116 KDA U5 SMALL NUCLEAR RIBONUCLEOPROTEIN

COMPONENT. [MASS = 109436

12
15

Homo
sapiens (Human) STRESS-INDUCED-PHOSPHOPROTEIN 1. [MASS = 62639

12
12

Homo
sapiens (Human) COATOMER SUBUNIT ALPHA. [MASS = 138332

11
14

Homo
sapiens (Human) T-COMPLEX PROTEIN 1 SUBUNIT DELTA. [MASS = 57793

11
17

Homo
sapiens (Human) ISOFORM 1 OF HETEROGENEOUS NUCLEAR

RIBONUCLEOPROTEIN D0. [MASS = 38434

11
12

Homo
sapiens (Human) CDNA FLJ45525 FIS, CLONE BRTHA2026311, HIGHLY SIMILAR

TO PROTEIN DISULFIDE ISOMERASE A6. [MASS = 53929

11
12

Homo
sapiens (Human) ATP-DEPENDENT RNA HELICASE DDX3X. [MASS = 73112

11
13

Homo
sapiens (Human) 26S PROTEASE REGULATORY SUBUNIT 6A. [MASS = 49204

11
15

Homo
sapiens (Human) GLYCOGEN PHOSPHORYLASE, BRAIN FORM. [MASS = 96565

11
12

Homo
sapiens (Human) PROTEASOME 26S NON-ATPASE SUBUNIT 11 VARIANT

(FRAGMENT). [MASS = 47535

11
11

Homo
sapiens (Human) DNA DAMAGE-BINDING PROTEIN 1. [MASS = 126968

11
11

Homo
sapiens (Human) RAS GTPASE-ACTIVATING-LIKE PROTEIN IQGAP1.

[MASS = 189252

11
15

Homo
sapiens (Human) STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING

PROTEIN 1. [MASS = 101997

11
23

Homo
sapiens (Human) UBIQUITIN-ACTIVATING ENZYME E1. [MASS = 56852

11
12

Homo
sapiens (Human) ISOFORM 2 OF PROTEIN KIAA1967. [MASS = 103030

11
11

Homo
sapiens (Human) ISOFORM 2 OF MICROTUBULE-ASSOCIATED PROTEIN 4.

[MASS = 102906

11
14

Homo
sapiens (Human) ISOFORM GTBP-ALT OF DNA MISMATCH REPAIR PROTEIN

MSH6. [MASS = 120563

11
14

Homo
sapiens (Human) 150 KDA OXYGEN-REGULATED PROTEIN PRECURSOR.

[MASS = 111335

11
11

Homo
sapiens (Human) TALDO1 PROTEIN. [MASS = 35329

11
24

Homo
sapiens (Human) STATHMIN. [MASS = 17171

11
11

Homo
sapiens (Human) ISOFORM 1 OF CHROMODOMAIN HELICASE-DNA-BINDING

PROTEIN 4. [MASS = 217991

10
11

Homo
sapiens (Human) RUVB-LIKE 2. [MASS = 51025

10
14

Homo
sapiens (Human) PLASMA PROTEASE C1 INHIBITOR PRECURSOR. [MASS = 55154

10
34

Homo
sapiens (Human) HEAT SHOCK PROTEIN HSP 90-ALPHA 2. [MASS = 98113

10
10

Homo
sapiens (Human) PIGMENT EPITHELIUM-DERIVED FACTOR PRECURSOR.

[MASS = 46342

10
11

Homo
sapiens (Human) ISOFORM LONG OF UBIQUITIN CARBOXYL-TERMINAL

HYDROLASE 5. [MASS = 95786

10
47

Homo
sapiens (Human) PRO2275. [MASS = 13097

10
12

Homo
sapiens (Human) T-COMPLEX PROTEIN 1 SUBUNIT ZETA. [MASS = 57893

10
36

Homo
sapiens (Human) MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE.

[MASS = 31423

10
10

Homo
sapiens (Human) TALIN-1. [MASS = 269767

10
11

Homo
sapiens (Human) VACUOLAR PROTEIN SORTING 35. [MASS = 91707

10
12

Homo
sapiens (Human) T-COMPLEX PROTEIN 1 SUBUNIT EPSILON. [MASS = 59671

10
11

Homo
sapiens (Human) ISOFORM 3 OF POLYPYRIMIDINE TRACT-BINDING PROTEIN 2.

[MASS = 58084

10
10

Homo
sapiens (Human) ISOFORM 1 OF REGULATOR OF NONSENSE TRANSCRIPTS 1.

[MASS = 124345

10
10

Homo
sapiens (Human) UBIQUITIN-LIKE 1-ACTIVATING ENZYME E1B. [MASS = 71224

10
16

Homo
sapiens (Human) ISOFORM 1 OF CLATHRIN HEAVY CHAIN 2. [MASS = 187030

10
12

Homo
sapiens (Human) SEPTIN-7. [MASS = 50809

10
14

Homo
sapiens (Human) ADENYLYL CYCLASE-ASSOCIATED PROTEIN 1. [MASS = 51542

10
10

Homo
sapiens (Human) VALYL-TRNA SYNTHETASE. [MASS = 140476

10
11

Homo
sapiens (Human) DNA MISMATCH REPAIR PROTEIN MSH2. [MASS = 104743

10
28

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN R.

[MASS = 70943

10
19

Homo
sapiens (Human) FRUCTOSE-BISPHOSPHATE ALDOLASE A. [MASS = 39289

10
15

Homo
sapiens (Human) THREONYL-TRNA SYNTHETASE, CYTOPLASMIC. [MASS = 83435

10
11

Homo
sapiens (Human) STRESS-70 PROTEIN, MITOCHONDRIAL PRECURSOR.

[MASS = 73680

10
11

Homo
sapiens (Human) ELONGATION FACTOR 1-DELTA. [MASS = 30991

10
11

Homo
sapiens (Human) CYTOSKELETON-ASSOCIATED PROTEIN 5. [MASS = 225509

10
11

Homo
sapiens (Human) CALRETICULIN PRECURSOR. [MASS = 48142

10
13

Homo
sapiens (Human) PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE.

[MASS = 144664

10
11

Homo
sapiens (Human) ISOFORM 4 OF TUBULIN-SPECIFIC CHAPERONE D.

[MASS = 138597

10
13

Homo
sapiens (Human) ISOFORM B OF ARSENITE-RESISTANCE PROTEIN 2.

[MASS = 100276

10
10

Homo
sapiens (Human) ISOFORM 2 OF STRUCTURAL MAINTENANCE OF

CHROMOSOMES 4-LIKE 1 PROTEIN. [MASS = 140278

10
13

Homo
sapiens (Human) ISOFORM 1 OF RETICULON-4. [MASS = 130102

10
45

Homo
sapiens (Human) HEMOGLOBIN SUBUNIT ZETA. [MASS = 15506

10
11

Homo
sapiens (Human) HSPC117 PROTEIN. [MASS = 55210

10
10

Homo
sapiens (Human) ISOFORM 1 OF SQUAMOUS CELL CARCINOMA ANTIGEN

RECOGNIZED BY T-CELLS 3. [MASS = 109935

10
14

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S4, X ISOFORM. [MASS = 29467

10
10

Homo
sapiens (Human) TRANSKETOLASE. [MASS = 67878

9
13

Homo
sapiens (Human) ISOFORM 2 OF NUCLEOPHOSMIN. [MASS = 29465

9
10

Homo
sapiens (Human) UNCHARACTERIZED PROTEIN C20ORF77. [MASS = 36900

9
9

Homo
sapiens (Human) T-COMPLEX PROTEIN 1 SUBUNIT ALPHA. [MASS = 60344

9
10

Homo
sapiens (Human) INTERLEUKIN ENHANCER-BINDING FACTOR 2. [MASS = 43062

9
10

Homo
sapiens (Human) 26S PROTEASE REGULATORY SUBUNIT 8. [MASS = 45626

9
11

Homo
sapiens (Human) ISOFORM 1 OF DNA REPLICATION LICENSING FACTOR

MCM7. [MASS = 81308

9
19

Homo
sapiens (Human) GAMMA-ENOLASE. [MASS = 47137

9
11

Homo
sapiens (Human) HYPOTHETICAL PROTEIN DKFZP451D234. [MASS = 109187

9
23

Homo
sapiens (Human) TUBULIN, BETA 2. [MASS = 49907

9
11

Homo
sapiens (Human) ISOFORM 1 OF PROTEIN ARGININE

N-METHYLTRANSFERASE 1. [MASS = 41486

9
12

Homo
sapiens (Human) VILLIN 2. [MASS = 69413

9
11

Homo
sapiens (Human) RADIXIN. [MASS = 68564

9
11

Homo
sapiens (Human) ISOFORM 2 OF SWI/SNF-RELATED MATRIX-ASSOCIATED

ACTIN-DEPENDENT

REGULATOR OF CHROMATIN SUBFAMILY C MEMBER 2. [MASS = 124841

9
9

Homo
sapiens (Human) ASPARTYL-TRNA SYNTHETASE. [MASS = 57136

9
12

Homo
sapiens (Human) D-3-PHOSPHOGLYCERATE DEHYDROGENASE. [MASS = 56519

9
9

Homo
sapiens (Human) CAD PROTEIN. [MASS = 242984

9
9

Homo
sapiens (Human) CTP SYNTHASE 1. [MASS = 66690

9
9

Homo
sapiens (Human) SERINE-THREONINE KINASE RECEPTOR-ASSOCIATED

PROTEIN. [MASS = 38438

9
11

Homo
sapiens (Human) ADENOSYLHOMOCYSTEINASE. [MASS = 47585

9
11

Homo
sapiens (Human) ELONGATION FACTOR 1-GAMMA. [MASS = 49988

9
20

Homo
sapiens (Human) CDNA FLJ45706 FIS, CLONE FEBRA2028457, HIGHLY SIMILAR

TO NUCLEOLIN. [MASS = 65962

9
10

Homo
sapiens (Human) CHAPERONIN CONTAINING TCP1, SUBUNIT 3 ISOFORM B.

[MASS = 60463

9
12

Homo
sapiens (Human) ENO1P PROTEIN. [MASS = 42342

9
9

Homo
sapiens (Human) PREDICTED: SIMILAR TO HETEROGENEOUS NUCLEAR

RIBONUCLEOPROTEIN U. [MASS = 101752

9
11

Homo
sapiens (Human) ISOFORM SHORT OF RECEPTOR-TYPE TYROSINE-PROTEIN

PHOSPHATASE ZETA PRECURSOR. [MASS = 163444

9
11

Homo
sapiens (Human) 26S PROTEASE REGULATORY SUBUNIT 7. [MASS = 48503

9
10

Homo
sapiens (Human) PROTEIN DISULFIDE-ISOMERASE PRECURSOR. [MASS = 57116

9
11

Homo
sapiens (Human) FACT COMPLEX SUBUNIT SPT16. [MASS = 119914

9
14

Homo
sapiens (Human) LUMICAN PRECURSOR. [MASS = 38429

9
10

Homo
sapiens (Human) PROLIFERATION-ASSOCIATED PROTEIN 2G4. [MASS = 43656

9
10

Homo
sapiens (Human) IARS PROTEIN. [MASS = 120627

9
10

Homo
sapiens (Human) U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 200 KDA

HELICASE. [MASS = 244508

9
9

Homo
sapiens (Human) UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7.

[MASS = 128272

9
10

Homo
sapiens (Human) INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN

H1 PRECURSOR. [MASS = 101389

9
12

Homo
sapiens (Human) ATP-DEPENDENT RNA HELICASE DDX1. [MASS = 82432

9
13

Homo
sapiens (Human) ISOFORM 1 OF PROBABLE ATP-DEPENDENT RNA

HELICASE DDX17. [MASS = 72371

9
10

Homo
sapiens (Human) RNA-BINDING PROTEIN 12. [MASS = 97395

9
9

Homo
sapiens (Human) TRANSPORTIN 1. [MASS = 102355

9
9

Homo
sapiens (Human) GTP-BINDING NUCLEAR PROTEIN RAN. [MASS = 24292

9
9

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S18. [MASS = 17719

9
30

Homo
sapiens (Human) ACTIN, AORTIC SMOOTH MUSCLE [MASS = 42009

9
10

Homo
sapiens (Human) EUKARYOTIC TRANSLATION INITIATION FACTOR 4

GAMMA 2. [MASS = 102362

9
12

Homo
sapiens (Human) SPLICING FACTOR, ARGININE/SERINE-RICH 1. [MASS = 27745

9
9

Homo
sapiens (Human) PEROXIREDOXIN-1. [MASS = 22110

8
10

Homo
sapiens (Human) GLUTATHIONE S-TRANSFERASE P. [MASS = 23225

8
31

Homo
sapiens (Human) ACTIN, CYTOPLASMIC 1. [MASS = 41737

8
9

Homo
sapiens (Human) HEAT SHOCK 70 KDA PROTEIN 1L. [MASS = 70375

8
12

Homo
sapiens (Human) COFILIN-1. [MASS = 18371

8
13

Homo
sapiens (Human) ANGIOTENSINOGEN PRECURSOR. [MASS = 53154

8
10
Hoino sapiens (Human) GLUCOSE-6-PHOSPHATE ISOMERASE. [MASS = 63016

8
51

Homo
sapiens (Human) TUBULIN BETA-2 CHAIN. [MASS = 49671

8
12

Homo
sapiens (Human) ISOFORM 1 OF CYTOSOLIC ACYL COENZYME A THIOESTER

HYDROLASE. [MASS = 41796

8
10

Homo
sapiens (Human) HEAT SHOCK 70 KDA PROTEIN 1. [MASS = 70052

8
9

Homo
sapiens (Human) 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12.

[MASS = 52773

8
23

Homo
sapiens (Human) GAMMA-G GLOBIN (FRAGMENT). [MASS = 16969

8
9

Homo
sapiens (Human) 14-3-3 PROTEIN THETA. WASS = 27764

8
8

Homo
sapiens (Human) MALATE DEHYDROGENASE, MITOCHONDRIAL PRECURSOR.

[MASS = 35531

8
9

Homo
sapiens (Human) A-KINASE ANCHOR PROTEIN 12 ISOFORM 2. [MASS = 181690

8
8

Homo
sapiens (Human) LIVER PHOSPHOFRUCTOKINASE ISOFORM A. [MASS = 90577

8
14

Homo
sapiens (Human) 14-3-3 PROTEIN ZETA/DELTA. [MASS = 27745

8
10

Homo
sapiens (Human) ATP-DEPENDENT RNA HELICASE A. [MASS = 140881

8
15

Homo
sapiens (Human) ISOFORM C1 OF HETEROGENEOUS NUCLEAR

RIBONUCLEOPROTEINS C1/C2. [MASS = 32338

8
8

Homo
sapiens (Human) NUCLEOSIDE DIPHOSPHATE KINASE A. [MASS = 17149

8
8

Homo
sapiens (Human) TRIPEPTIDYL-PEPTIDASE 2. [MASS = 138219

8
11

Homo
sapiens (Human) RCTPI1 (FRAGMENT). [MASS = 26943

8
11

Homo
sapiens (Human) ISOFORM 1 OF POLYPYRIMIDINE TRACT-BINDING PROTEIN 1.

[MASS = 57221

8
11

Homo
sapiens (Human) CELLULAR RETINOIC ACID-BINDING PROTEIN 1. [MASS = 15434

8
8

Homo
sapiens (Human) MALATE DEHYDROGENASE, CYTOPLASMIC. [MASS = 36295

8
10

Homo
sapiens (Human) ESTERASE D. [MASS = 31463

8
15

Homo
sapiens (Human) ISOFORM 1 OF HETEROGENEOUS NUCLEAR

RIBONUCLEOPROTEIN Q. [MASS = 69603

8
12

Homo
sapiens (Human) ISOFORM SHORT OF RNA-BINDING PROTEIN FUS. [MASS = 53355

8
9

Homo
sapiens (Human) F-ACTIN CAPPING PROTEIN ALPHA-1 SUBUNIT. [MASS = 32792

8
10

Homo
sapiens (Human) PROBABLE ATP-DEPENDENT RNA HELICASE DDX48. [MASS = 46740

8
10

Homo
sapiens (Human) INORGANIC PYROPHOSPHATASE. [MASS = 32660

8
21

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A0. [MASS = 30841

8
9

Homo
sapiens (Human) ISOFORM 1 OF GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE

AMINOTRANSFERASE [ISOMERIZING] 1. [MASS = 78806

8
10

Homo
sapiens (Human) ELAV. [MASS = 42417

8
8

Homo
sapiens (Human) CDNA FLJ33352 FIS, CLONE BRACE2005087, WEAKLY SIMILAR

TO PRE-MRNA SPLICING HELICASE BRR2. [MASS = 71472

8
8

Homo
sapiens (Human) PREDICTED: SIMILAR TO ATP-DEPENDENT DNA HELICASE II,

70 KDA SUBUNIT (LUPUS KU AUTOANTIGEN PROTEIN P70) (KU70) (70 KDA SUBUNIT OF KU

ANTIGEN) (THYROID-LUPUS AUTOANTIGEN) (TLAA) (CTC BOX BINDING FACTOR 75 KDA

SUBUNIT) (CTCBF) (CTC75) ISOFORM 1. [MASS = 54430

8
11

Homo
sapiens (Human) IMPORTIN-7. [MASS = 119517

8
8

Homo
sapiens (Human) ISOFORM 1 OF CULLIN-3. [MASS = 88930

8
8

Homo
sapiens (Human) 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 6. [MASS = 45531

8
9

Homo
sapiens (Human) PROTEIN PHOSPHATASE 2C ISOFORM GAMMA. [MASS = 59272

8
24

Homo
sapiens (Human) HYPOTHETICAL PROTEIN LOC345651. [MASS = 42003

8
9

Homo
sapiens (Human) 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3. [MASS = 60978

8
10

Homo
sapiens (Human) ISOFORM 1 OF EUKARYOTIC TRANSLATION INITIATION FACTOR 3

SUBUNIT 9. [MASS = 92492

8
9

Homo
sapiens (Human) 60S ACIDIC RIBOSOMAL PROTEIN P0. [MASS = 34274

8
8

Homo
sapiens (Human) FACT COMPLEX SUBUNIT SSRP1. [MASS = 81075

8
12

Homo
sapiens (Human) NUCLEOSOME ASSEMBLY PROTEIN 1-LIKE 4. [MASS = 43011

8
12

Homo
sapiens (Human) ISOFORM BETA-2 OF DNA TOPOISOMERASE 2-BETA. [MASS = 183267

8
9

Homo
sapiens (Human) GARS PROTEIN. [MASS = 84648

8
8

Homo
sapiens (Human) STRUCTURAL MAINTENANCE OF CHROMOSOME 1-LIKE 1 PROTEIN.

[MASS = 143233

8
8

Homo
sapiens (Human) DNA-DIRECTED RNA POLYMERASE II 140 KDA POLYPEPTIDE.

[MASS = 133897

8
24

Homo
sapiens (Human) NESTIN. [MASS = 177439

8
8

Homo
sapiens (Human) PREDICTED: SIMILAR TO PEPTIDYLPROLYL ISOMERASE

A ISOFORM 1. [MASS = 24517

8
11

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN D-LIKE. [MASS = 46438

8
10

Homo
sapiens (Human) ISOFORM 2C OF CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN 2.

[MASS = 68426

7
7

Homo
sapiens (Human) CSNK2A1 PROTEIN. [MASS = 45909

7
7

Homo
sapiens (Human) PEROXIREDOXIN-6. [MASS = 24904

7
9

Homo
sapiens (Human) HIGH MOBILITY GROUP PROTEIN 1-LIKE 10. [MASS = 24218

7
10

Homo
sapiens (Human) CALMODULIN. [MASS = 16706

7
9

Homo
sapiens (Human) NUCLEOSOME ASSEMBLY PROTEIN 1-LIKE 1. [MASS = 45374

7
10

Homo
sapiens (Human) IMPORTIN-9. [MASS = 115832

7
13

Homo
sapiens (Human) ISOFORM 1 OF PROTEIN SET. [MASS = 33489

7
11

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN H1. [MASS = 49229

7
9

Homo
sapiens (Human) SEPTIN-2. [MASS = 41487

7
7

Homo
sapiens (Human) ATAXIN-10. [MASS = 53489

7
7

Homo
sapiens (Human) COATOMER SUBUNIT BETA. [MASS = 107139

7
9

Homo
sapiens (Human) ISOFORM 2 OF DNA REPLICATION LICENSING FACTOR MCM7.

[MASS = 44649

7
7

Homo
sapiens (Human) IRON-RESPONSIVE ELEMENT-BINDING PROTEIN 1. [MASS = 98399

7
10

Homo
sapiens (Human) TYROSINE 3-MONOOXYGENASE/TRYPTOPHAN 5-MONOOXYGENASE

ACTIVATION PROTEIN, BETA POLYPEPTIDE. [MASS = 28082

7
9

Homo
sapiens (Human) 26S PROTEASE REGULATORY SUBUNIT S10B. [MASS = 44173

7
8

Homo
sapiens (Human) HYPOTHETICAL PROTEIN DKFZP781K0743. [MASS = 105850

7
8

Homo
sapiens (Human) UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L1.

[MASS = 24824

7
9

Homo
sapiens (Human) ISOFORM 1 OF HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN

U-LIKE PROTEIN 1. [MASS = 95739

7
8

Homo
sapiens (Human) TUBULIN-TYROSINE LIGASE-LIKE PROTEIN 12. [MASS = 74404

7
7

Homo
sapiens (Human) ISOFORM 2 OF NUCLEAR MITOTIC APPARATUS PROTEIN 1.

[MASS = 236531

7
8

Homo
sapiens (Human) ALPHA ISOFORM OF REGULATORY SUBUNIT A, PROTEIN

PHOSPHATASE 2. [MASS = 65309

7
8

Homo
sapiens (Human) ISOFORM 4 OF HETEROGENEOUS NUCLEAR

RIBONUCLEOPROTEIN A/B. [MASS = 31233

7
8

Homo
sapiens (Human) CCR4-NOT TRANSCRIPTION COMPLEX, SUBUNIT 1 ISOFORM A.

[MASS = 266939

7
8

Homo
sapiens (Human) PROFILIN-1. [MASS = 14923

7
11

Homo
sapiens (Human) PROLIFERATING CELL NUCLEAR ANTIGEN. [MASS = 28769

7
7

Homo
sapiens (Human) METHIONYL-TRNA SYNTHETASE. [MASS = 101116

7
8

Homo
sapiens (Human) UBIQUITIN-LIKE 1-ACTIVATING ENZYME E1A. [MASS = 38450

7
8

Homo
sapiens (Human) ALCOHOL DEHYDROGENASE. [MASS = 36442

7
7

Homo
sapiens ,(Human) ADP-RIBOSYLATION FACTOR 1. [MASS = 20566

7
9

Homo
sapiens (Human) THIOREDOXIN-LIKE PROTEIN 1. [MASS = 32120

7
11

Homo
sapiens (Human) ISOFORM 1 OF 26S PROTEASOME NON-ATPASE REGULATORY

SUBUNIT 1. [MASS = 105836

7
7

Homo
sapiens (Human) EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 8.

[MASS = 105344

7
7

Homo
sapiens (Human) VESICLE-FUSING ATPASE. [MASS = 82654

7
8

Homo
sapiens (Human) Complement component 3 precursor. [MASS = 187306

7
7

Homo
sapiens (Human) EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 6.

[MASS = 52221

7
8

Homo
sapiens (Human) ATP-DEPENDENT DNA HELICASE 2 SUBUNIT 1. [MASS = 69712

7
7

Homo
sapiens (Human) ISOFORM 1 OF HOST CELL FACTOR. [MASS = 208842

7
7

Homo
sapiens (Human) METASTASIS-ASSOCIATED PROTEIN MTA2. [MASS = 75023

7
7

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN L ISOFORM

A. [MASS = 64133

7
8

Homo
sapiens (Human) 26S PROTEASE REGULATORY SUBUNIT 4. [MASS = 49185

7
8

Homo
sapiens (Human) UDP-GLUCOSE CERAMIDE GLUCOSYLTRANSFERASE-LIKE 1

ISOFORM 1. [MASS = 177190

7
7

Homo
sapiens (Human) EUKARYOTIC TRANSLATION INITIATION FACTOR 5B. [MASS = 138800

7
7

Homo
sapiens (Human) ISOFORM V0 OF VERSICAN CORE PROTEIN PRECURSOR. [MASS = 372820

7
26

Homo
sapiens (Human) TUBULIN BETA-1 CHAIN. [MASS = 50327

7
7

Homo
sapiens (Human) ISOFORM 1 OF FILAMIN-B. [MASS = 278195

7
7

Homo
sapiens (Human) CONTACTIN-2 PRECURSOR. [MASS = 113393

7
7

Homo
sapiens (Human) UBIQUITIN SPECIFIC PROTEASE 9, X-LINKED ISOFORM 4. [MASS = 290497

6
11

Homo
sapiens (Human) ISOFORM 1 OF HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN

A3. [MASS = 39595

6
11

Homo
sapiens (Human) HEMOGLOBIN SUBUNIT ALPHA: [MASS = 15126

6
8

Homo
sapiens (Human) ASTROCYTIC PHOSPHOPROTEIN PEA-15. [MASS = 15040

6
6

Homo
sapiens (Human) 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 7. [MASS = 37025

6
7

Homo
sapiens (Human) PROTEASOME SUBUNIT BETA TYPE 1. [MASS = 26489

6
6

Homo
sapiens (Human) ALPHA-CENTRACTIN. [MASS = 42614

6
10

Homo
sapiens (Human) ISOFORM 1 OF PLASMINOGEN ACTIVATOR INHIBITOR 1

RNA-BINDING PROTEIN. [MASS = 44965

6
7

Homo
sapiens (Human) CALPONIN-3. [MASS = 36414

6
7

Homo
sapiens (Human) PROTEASOME SUBUNIT ALPHA TYPE 2. [MASS = 25767

6
8

Homo
sapiens (Human) PHOSPHATIDYLETHANOLAMINE-BINDING. PROTEIN 1. [MASS = 20926

6
6

Homo
sapiens (Human) METHIONINE ADENOSYLTRANSFERASE II, BETA ISOFORM 1.

[MASS = 37552

6
12

Homo
sapiens (Human) VITRONECTIN PRECURSOR. [MASS = 54306

6
7

Homo
sapiens (Human) PHOSPHORIBOSYL PYROPHOSPHATE SYNTHETASE-ASSOCIATED

PROTEIN 2. [MASS = 40926

6
6

Homo
sapiens (Human) CONDENSIN COMPLEX SUBUNIT 3. [MASS = 114334

6
10

Homo
sapiens (Human) PEROXIREDOXIN-2. [MASS = 21761

6
12

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1

ISOFORM B. [MASS = 38747

6
8

Homo
sapiens (Human) RHO GDP-DISSOCIATION INHIBITOR 1. [MASS = 23076

6
6

Homo
sapiens (Human) ISOFORM 1 OF DIPEPTIDYL-PEPTIDASE 3. [MASS = 82589

6
6

Homo
sapiens (Human) ISOFORM 1 OF ACTIN-LIKE PROTEIN 6A. [MASS = 47461

6
6

Homo
sapiens (Human) ISOFORM 2 OF NSFL1 COFACTOR P47. [MASS = 37325

6
6

Homo
sapiens (Human) EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA,

1 ISOFORM 2. [MASS = 166589

6
53

Homo
sapiens (Human) ELONGATION FACTOR 1-ALPHA 2. [MASS = 50470

6
8

Homo
sapiens (Human) HYPOTHETICAL PROTEIN DKFZP564E242. [MASS = 31424

6
6

Homo
sapiens (Human) 3-MERCAPTOPYRUVATE SULFURTRANSFERASE. [MASS = 33047

6
7

Homo
sapiens (Human) CLUSTERIN PRECURSOR. [MASS = 52495

6
6

Homo
sapiens (Human) REPLICATION PROTEIN A 70 KDA DNA-BINDING SUBUNIT. [MASS = 68138

6
7

Homo
sapiens (Human) DYNACTIN 2. [MASS = 44820

6
7

Homo
sapiens (Human) THIOREDOXIN-LIKE PROTEIN 2. [MASS = 37432

6
6

Homo
sapiens (Human) ISOFORM 2 OF CADHERIN-11 PRECURSOR. [MASS = 76541

6
9

Homo
sapiens (Human) S-ADENOSYLMETHIONINE SYNTHETASE ISOFORM TYPE-2. [MASS = 43661

6
7

Homo
sapiens (Human) ISOFORM 1 OF ELAV-LIKE PROTEIN 3. [MASS = 39547

6
6

Homo
sapiens (Human) SIMILAR TO ANNEXIN A2 ISOFORM 1. [MASS = 38659

6
8

Homo
sapiens (Human) ASPARTATE AMINOTRANSFERASE, CYTOPLASMIC. [MASS = 46116

6
7

Homo
sapiens (Human) NUCLEAR MIGRATION PROTEIN NUDC. [MASS = 38243

6
7

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S19. [MASS = 15929

6
6

Homo
sapiens (Human) RNA BINDING PROTEIN (FRAGMENT). [MASS = 32550

6
6

Homo
sapiens (Human) ISOFORM 1 OF DNA. [MASS = 189566

6
9

Homo
sapiens (Human) ISOFORM 2 OF SERINE/THREONINE-PROTEIN KINASE DCAMKL1.

[MASS = 82224

6
6

Homo
sapiens (Human) PROTEIN TRANSPORT PROTEIN SEC23A. [MASS = 86147

6
10

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L4. [MASS = 47566

6
7

Homo
sapiens (Human) PROTEASOME 26S NON-ATPASE SUBUNIT 13 ISOFORM 2. [MASS = 39871

6
6

Homo
sapiens (Human) ISOFORM 4 OF AFADIN. [MASS = 206804

6
16

Homo
sapiens (Human) DIHYDROPYRIMIDINASE-RELATED PROTEIN 2. [MASS = 62294

6
6

Homo
sapiens (Human) WUGSC:H_RG054D04.1 PROTEIN. [MASS = 29037

6
7

Homo
sapiens (Human) VACUOLAR ATP SYNTHASE CATALYTIC SUBUNIT A, UBIQUITOUS

ISOFORM. [MASS = 68304

6
6

Homo
sapiens (Human) MGEA5 PROTEIN. [MASS = 95331

6
7

Homo
sapiens (Human) GLUCOSIDASE 2 SUBUNIT BETA PRECURSOR. [MASS = 59296

6
6

Homo
sapiens (Human) CYSTEINYL-TRNA SYNTHETASE ISOFORM C.- [MASS = 94638

6
9

Homo
sapiens (Human) BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH. [MASS = 64616

6
6

Homo
sapiens (Human) HSC70-INTERACTING PROTEIN. [MASS = 41332

6
7

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S3. [MASS = 26688

6
9

Homo
sapiens (Human) GALECTIN-3-BINDING PROTEIN PRECURSOR. [MASS = 65331

6
6

Homo
sapiens (Human) ISOFORM 1 OF COMPLEMENT FACTOR B PRECURSOR (FRAGMENT).

[MASS = 85533

6
6

Homo
sapiens (Human) ISOFORM 1 OF POLYADENYLATE-BINDING PROTEIN 4. [MASS = 70783

6
6

Homo
sapiens (Human) SPERMIDINE SYNTHASE. [MASS = 33825

6
7

Homo
sapiens (Human) PROTEIN DJ-1. [MASS = 19891

6
8

Homo
sapiens (Human) ISOFORM 1 OF HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN

H3. [MASS = 36926

6
8

Homo
sapiens (Human) RAB1A, MEMBER RAS ONCOGENE FAMILY. [MASS = 22678

6
6

Homo
sapiens (Human) WD40 PROTEIN. [MASS = 35079

6
11

Homo
sapiens (Human) ISOFORM B1 OF HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS

A2/B1. [MASS = 37430

6
7

Homo
sapiens (Human) RUVB-LIKE 1. [MASS = 50228

6
10

Homo
sapiens (Human) RNA BINDING MOTIF PROTEIN, X-LINKED-LIKE 1. [MASS = 42142

6
7

Homo
sapiens (Human) PHOSPHOGLYCERATE MUTASE 2. [MASS = 28635

6
7

Homo
sapiens (Human) DNA POLYMERASE DELTA CATALYTIC SUBUNIT. [MASS = 123631

6
6

Homo
sapiens (Human) SF3B3 PROTEIN. [MASS = 30210

6
6

Homo
sapiens (Human) RETINOBLASTOMA-ASSOCIATED FACTOR 600. [MASS = 185447

5
35
TRYPSIN PRECURSOR (EC 3.4.21.4) > PIR1:TRPGTR trypsin (EC 3.4.21.4)

5
8

Homo
sapiens (Human) TUBULIN BETA-4 CHAIN. [MASS = 49586

5
18

Homo
sapiens (Human) PROTHYMOSIN ALPHA. [MASS = 12203

5
6

Homo
sapiens (Human) 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 14. [MASS = 34577

5
7

Homo
sapiens (Human) GLYOXYLATE REDUCTASE/HYDROXYPYRUVATE REDUCTASE.

[MASS = 35668

5
7

Homo
sapiens (Human) DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE. [MASS = 35423

5
6

Homo
sapiens (Human) ISOFORM 2 OF GUANINE NUCLEOTIDE-BINDING PROTEIN G(I),

ALPHA-2 SUBUNIT. [MASS = 38473

5
5

Homo
sapiens (Human) ISOFORM 1 OF PROTEIN 4. 1. [MASS = 97017

5
7

Homo
sapiens (Human) ADP-SUGAR PYROPHOSPHATASE. [MASS = 24328

5
6

Homo
sapiens (Human) HISTONE H2B TYPE 2-E. [MASS = 13789

5
10

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN F. [MASS = 45672

5
9

Homo
sapiens (Human) PREDICTED: SIMILAR TO HETEROGENEOUS NUCLEAR

RIBONUCLEOPROTEIN A3 ISOFORM 1. [MASS = 31312

5
9

Homo
sapiens (Human) NG, NG-DIMETHYLARGININE DIMETHYLAMINOHYDROLASE 2.

[MASS = 29644

5
5

Homo
sapiens (Human) SERYL-TRNA SYNTHETASE. [MASS = 58646

5
5

Homo
sapiens (Human) NUCLEASE SENSITIVE ELEMENT-BINDING PROTEIN 1.

[MASS = 35793

5
10

Homo
sapiens (Human) ISOFORM 1 OF CLEAVAGE AND POLYADENYLATION SPECIFICITY

FACTOR 6. [MASS = 59210

5
7

Homo
sapiens (Human) POLY(RC)-BINDING PROTEIN 1. [MASS = 37498

5
7

Homo
sapiens (Human) ISOFORM B OF FIBULIN-1 PRECURSOR. [MASS = 77186

5
5

Homo
sapiens (Human) FK506-BINDING PROTEIN 3. [MASS = 25177

5
5

Homo
sapiens (Human) CTTN PROTEIN. [MASS = 70959

5
14

Homo
sapiens (Human) PREDICTED: SIMILAR TO HETEROGENEOUS NUCLEAR

RIBONUCLEOPROTEIN A1. [MASS = 32163

5
5

Homo
sapiens (Human) PRE-MRNA-SPLICING FACTOR 19. [MASS = 55181

5
7

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S15. [MASS = 16909

5
6

Homo
sapiens (Human) GLUTAMINYL-TRNA SYNTHETASE. [MASS = 87799

5
5

Homo
sapiens (Human) PROTEIN RCC2. [MASS = 56085

5
8

Homo
sapiens (Human) DOUBLECORTEX\; LISSENCEPHALY, X-LINKED. [MASS = 49847

5
5

Homo
sapiens (Human) ISOFORM B OF MANNOSE-6-PHOSPHATE RECEPTOR-BINDING

PROTEIN 1. [MASS = 47047

5
6

Homo
sapiens (Human) COATOMER SUBUNIT BETA’. [MASS = 102356

5
7

Homo
sapiens (Human) HEPATOMA-DERIVED GROWTH FACTOR. [MASS = 26788

5
7

Homo
sapiens (Human) ELAV-LIKE PROTEIN 1. [MASS = 36092

5
5

Homo
sapiens (Human) PP856. [MASS = 43833

5
7

Homo
sapiens (Human) RAS-RELATED PROTEIN RAB-2A. [MASS = 23546

5
6

Homo
sapiens (Human) ISOFORM 2 OF PROTEIN ENABLED HOMOLOG. [MASS = 63924

5
7

Homo
sapiens (Human) ISOFORM 1 OF BETA-CATENIN. [MASS = 85497

5
5

Homo
sapiens (Human) AP-2 COMPLEX SUBUNIT ALPHA-2. [MASS = 104150

5
20

Homo
sapiens (Human) EEF1A1 PROTEIN. [MASS = 47869

5
7

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S17. [MASS = 15419

5
6

Homo
sapiens (Human) 14-3-3 PROTEIN GAMMA. [MASS = 28171

5
5

Homo
sapiens (Human) RAS-GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN 1. [MASS = 52164

5
11

Homo
sapiens (Human) POLY(RC)-BINDING PROTEIN 2 ISOFORM B. [MASS = 38222

5
5

Homo
sapiens (Human) EXPORTIN-T. [MASS = 109964

5
6

Homo
sapiens (Human) EUKARYOTIC TRANSLATION INITIATION FACTOR 2C 1. [MASS = 97214

5
5

Homo
sapiens (Human) PREDICTED: STRUCTURAL MAINTENANCE OF CHROMOSOMES

FLEXIBLE HINGE DOMAIN CONTAINING 1. [MASS = 220242

5
13

Homo
sapiens (Human) PREGNANCY ZONE PROTEIN PRECURSOR. [MASS = 163836

5
6

Homo
sapiens (Human) ISOFORM 1 OF LIM AND SH3 DOMAIN PROTEIN 1. [MASS = 29717

5
16

Homo
sapiens (Human) SIMILAR TO NESTIN. [MASS = 175922

5
5

Homo
sapiens (Human) ISOFORM 1 OF FILAMIN-C. [MASS = 291293

5
6

Homo
sapiens (Human) MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER 1.

[MASS = 29868

5
5

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S13. [MASS = 17091

5
5

Homo
sapiens (Human) MITOGEN-ACTIVATED PROTEIN KINASE 1. [MASS = 41259

5
5

Homo
sapiens (Human) UDP-GLUCOSE 6-DEHYDROGENASE. [MASS = 55024

5
9

Homo
sapiens (Human) PROBABLE ATP-DEPENDENT RNA HELICASE DDX5. [MASS = 69148

5
5

Homo
sapiens (Huirian) ISOFORM 1 OF SLIT-ROBO RHO GTPASE-ACTIVATING PROTEIN 3.

[MASS = 124504

5
5

Homo
sapiens (Human) HSP90 CO-CHAPERONE CDC37. [MASS = 44468

5
8

Homo
sapiens (Human) AMBP PROTEIN PRECURSOR. [MASS = 38999

5
5

Homo
sapiens (Human) CGI-150 PROTEIN. [MASS = 55012

5
6

Homo
sapiens (Human) PRE-MRNA-PROCESSING FACTOR 6 HOMOLOG. [MASS = 106925

5
5

Homo
sapiens (Human) TAR DNA-BINDING PROTEIN 43. [MASS = 44740

5
6

Homo
sapiens (Human) ISOFORM 1 OF KH DOMAIN-CONTAINING, RNA-BINDING, SIGNAL

TRANSDUCTION-ASSOCIATED PROTEIN 1. [MASS = 48227

5
5

Homo
sapiens (Human) HYPOTHETICAL PROTEIN DKFZP451P021. [MASS = 117896

5
7

Homo
sapiens (Human) KH-TYPE SPLICING REGULATORY PROTEIN. [MASS = 73115

5
5

Homo
sapiens (Human) ISOCITRATE DEHYDROGENASE [NADP], MITOCHONDRIAL

PRECURSOR. [MASS = 50909

5
5

Homo
sapiens (Human) 182 KDA TANKYRASE 1-BINDING PROTEIN. [MASS = 181816

5
5

Homo
sapiens (Human) CONDENSIN COMPLEX SUBUNIT 1. [MASS = 157169

5
7

Homo
sapiens (Human) ISOFORM 1 OF ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN

32 FAMILY MEMBER B. [MASS = 28788

5
5

Homo
sapiens (Human) SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2. [MASS = 13527

5
5

Homo
sapiens (Human) FUSE-BINDING PROTEIN-INTERACTING REPRESSOR ISOFORM A.

[MASS = 59875

5
6

Homo
sapiens (Human) COP9 SIGNALOSOME COMPLEX SUBUNIT 6. [MASS = 36163

5
5

Homo
sapiens (Human) ACETYL-COA CARBOXYLASE 1. [MASS = 265040

5
5

Homo
sapiens (Human) ISOFORM 2 OF SUPPRESSOR OF G2 ALLELE OF SKP1 HOMOLOG.

[MASS = 37673

5
5

Homo
sapiens (Human) ISOFORM 5 OF DYNAMIN-1-LIKE PROTEIN. [MASS = 79123

5
5

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L5. [MASS = 34231

5
6

Homo
sapiens (Human) PREDICTED: SIMILAR TO RIBOSOMAL PROTEIN L13 ISOFORM 1.

[MASS = 24280

5
5

Homo
sapiens (Human) INOSINE-5′-MONOPHOSPHATE DEHYDROGENASE 2. [MASS = 55805

5
5

Homo
sapiens (Human) ISOFORM 1 OF PHOSPHOSERINE AMINOTRANSFERASE. [MASS = 40423

5
9

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN C-LIKE 1.

[MASS = 32142

5
6

Homo
sapiens (Human) UBIQUITIN-CONJUGATING ENZYME E2 N. [MASS = 17138

5
5

Homo
sapiens (Human) PROTEIN KINASE C-BINDING PROTEIN NELL2 PRECURSOR.

[MASS = 91346

5
5

Homo
sapiens (Human) ISOFORM 1 OF DYNAMIN-2. [MASS = 98064

5
5

Homo
sapiens (Human) NEURONAL PROTEIN NP25. [MASS = 24893

5
5

Homo
sapiens (Human) SMALL GLUTAMINE-RICH TETRATRICOPEPTIDE

REPEAT-CONTAINING PROTEIN A. [MASS = 34063

4
4

Homo
sapiens (Human) PROLYL ENDOPEPTIDASE. [MASS = 80764

4
6

Homo
sapiens (Human) 60S ACIDIC RIBOSOMAL PROTEIN P2. [MASS = 11665

4
4

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S7. [MASS = 22127

4
6

Homo
sapiens (Human) ISOFORM 1 OF DNA-BINDING PROTEIN A. [MASS = 40090

4
6

Homo
sapiens (Human) ISOFORM EWS-B OF RNA-BINDING PROTEIN EWS. [MASS = 61217

4
4

Homo
sapiens (Human) THYMIDYLATE SYNTHASE. [MASS = 31759

4
5

Homo
sapiens (Human) NASCENT POLYPEPTIDE-ASSOCIATED COMPLEX SUBUNIT

ALPHA. [MASS = 23384

4
5

Homo
sapiens (Human) HISTIDINE-RICH GLYCOPROTEIN PRECURSOR. [MASS = 59578

4
4

Homo
sapiens (Human) ISOFORM 1 OF PROTEASOME SUBUNIT ALPHA TYPE 7. [MASS = 27887

4
4

Homo
sapiens (Human) 1-PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE PHOSPHODIESTERASE

GAMMA 1. [MASS = 148532

4
4

Homo
sapiens (Human) SPLICEOSOME RNA HELICASE BAT1. [MASS = 48991

4
5

Homo
sapiens (Human) PREDICTED: SIMILAR TO PHOSPHOGLYCERATE MUTASE 1

(PHOSPHOGLYCERATE MUTASE ISOZYME B) (PGAM-B) (BPG-DEPENDENT PGAM 1)

ISOFORM 1. [MASS = 28850

4
4

Homo
sapiens (Human) CRK-LIKE PROTEIN. [MASS = 33777

4
5

Homo
sapiens (Human) RNA-BINDING PROTEIN MUSASHI HOMOLOG 1. [MASS = 39125

4
4

Homo
sapiens (Human) FLAP ENDONUCLEASE 1. [MASS = 42593

4
5

Homo
sapiens (Human) ISOFORM C OF FIBULIN-1 PRECURSOR: [MASS = 74462

4
39

Homo
sapiens (Human) TUBA6 PROTEIN. [MASS = 37021

4
5

Homo
sapiens (Human) BA395L14.12. [MASS = 28403

4
4

Homo
sapiens (Human) ISOFORM SHORT OF TATA-BINDING PROTEIN-ASSOCIATED

FACTOR 2N. [MASS = 61558

4
5

Homo
sapiens (Human) LUNG-CANCER ONCOGENE 7. [MASS = 37889

4
4

Homo
sapiens (Human) DNAJ HOMOLOG SUBFAMILY A MEMBER 1. [MASS = 44868

4
5

Homo
sapiens (Human) CLASS III ALCOHOL DEHYDROGENASE 5 CHI SUBUNIT. [MASS = 41601

4
5

Homo
sapiens (Human) DYNC1H1 PROTEIN. [MASS = 22182

4
4

Homo
sapiens (Human) ISOFORM 2 OF SERINE/THREONINE-PROTEIN KINASE PAK 1. [MASS = 61632

4
5

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L21. [MASS = 18434

4
7

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN U ISOFORM A

[MASS = 90584

4
4

Homo
sapiens (Human) HISTONE H4. [MASS = 11236

4
4

Homo
sapiens (Human) SCC-112 PROTEIN. [MASS = 150830

4
4

Homo
sapiens (Human) WW DOMAIN-BINDING PROTEIN 11. [MASS = 69998

4
5

Homo
sapiens (Human) ISOFORM 2 OF PUTATIVE GTP-BINDING PROTEIN PTD004. [MASS = 27584

4
4

Homo
sapiens (Human) PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE, ISOFORM IB,

ALPHA SUBUNIT. [MASS = 46638

4
4

Homo
sapiens (Human) ISOFORM II OF UBIQUITIN-PROTEIN LIGASE E3A. [MASS = 100646

4
4

Homo
sapiens (Human) BRAIN ACID SOLUBLE PROTEIN 1. [MASS = 22562

4
7

Homo
sapiens (Human) ACTIN-LIKE PROTEIN 2. [MASS = 44761

4
5

Homo
sapiens (Human) EUKARYOTIC TRANSLATION INITIATION FACTOR 5. [MASS = 49223

4
4

Homo
sapiens (Human) 14-3-3 PROTEIN ETA. [MASS = 28088

4
4

Homo
sapiens (Human) EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1.

[MASS = 35981

4
5

Homo
sapiens (Human) ISOFORM DELTA-1 OF SERINE/THREONINE-PROTEIN PHOSPHATASE 2A

56 KDA REGULATORY SUBUNIT DELTA ISOFORM. [MASS = 69992

4
4

Homo
sapiens (Human) UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 10. [MASS = 87692

4
5

Homo
sapiens (Human) PDCD6IP PROTEIN. [MASS = 96818

4
4

Homo
sapiens (Human) PROTEIN FAM98B. [MASS = 37191

4
5

Homo
sapiens (Human) ISOFORM 1 OF 40S RIBOSOMAL PROTEIN S24. [MASS = 15423

4
5

Homo
sapiens (Human) ADENYLOSUCCINATE SYNTHETASE ISOZYME 2. [MASS = 50097

4
4

Homo
sapiens (Human) ISOFORM 1 OF MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY

MEMBER 2. [MASS = 37031

4
4

Homo
sapiens (Human) EXPORTIN-7. [MASS = 123776

4
4

Homo
sapiens (Human) PHOSPHOLIPASE A-2-ACTIVATING PROTEIN. [MASS = 87157

4
6

Homo
sapiens (Human) ISOFORM 1 OF PHOSPHOLIPID TRANSFER PROTEIN PRECURSOR.

[MASS = 54739

4
4

Homo
sapiens (Human) CERULOPLASMIN PRECURSOR. [MASS = 122205

4
5

Homo
sapiens (Human) RAS-RELATED PROTEIN RAB-7. [MASS = 23490

4
7

Homo
sapiens (Human) SERINE/THREONINE-PROTEIN PHOSPHATASE 2A CATALYTIC SUBUNIT

ALPHA ISOFORM. [MASS = 35594

4
4

Homo
sapiens (Human) ISOFORM 1 OF DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11A.

[MASS = 80593

4
6

Homo
sapiens (Human) ADENYLATE KINASE ISOENZYME 1. [MASS = 21635

4
5

Homo
sapiens (Human) GPI-ANCHORED PROTEIN P137. [MASS = 72752

4
6

Homo
sapiens (Human) ALPHA-2-ANTIPLASMIN PRECURSOR. [MASS = 55064

4
5

Homo
sapiens (Human) PLASMA RETINOL-BINDING PROTEIN PRECURSOR. [MASS = 23010

4
4

Homo
sapiens (Human) ISOFORM 4 OF SAPS DOMAIN FAMILY MEMBER 3. [MASS = 88952

4
5

Homo
sapiens (Human) AP-1 COMPLEX SUBUNIT MU-1. [MASS = 48456

4
4

Homo
sapiens (Human) ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN. [MASS = 33247

4
4

Homo
sapiens (Human) EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA, 1

ISOFORM 4. [MASS = 154805

4
4

Homo
sapiens (Human) PROTEASOME SUBUNIT ALPHA TYPE 6. [MASS = 27399

4
4

Homo
sapiens (Human) QUINONE OXIDOREDUCTASE. [MASS = 35207

4
4

Homo
sapiens (Human) SPLICING FACTOR 3A SUBUNIT 3. [MASS = 58849

4
4

Homo
sapiens (Human) GMP SYNTHASE. [MASS = 76715

4
4

Homo
sapiens (Human) ISOFORM 2 OF NMDA RECEPTOR-REGULATED PROTEIN 1. [MASS = 61602

4
4

Homo
sapiens (Human) EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 2.

[MASS = 36502

4
5

Homo
sapiens (Human) PROTEIN C14ORF166. [MASS = 28068

4
7

Homo
sapiens (Human) FARNESYL DIPHOSPHATE SYNTHASE. [MASS = 48275

4
5

Homo
sapiens (Human) COATOMER SUBUNIT GAMMA-2. [MASS = 97622

4
4

Homo
sapiens (Human) FIBRILLARIN. [MASS = 33784

4
4

Homo
sapiens (Human) NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1. [MASS = 91839

4
4

Homo
sapiens (Human) PROTEASOME SUBUNIT BETA TYPE 4 PRECURSOR. [MASS = 29192

4
4

Homo
sapiens (Human) CALCIUM-BINDING PROTEIN 39. [MASS = 39869

4
4

Homo
sapiens (Human) PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN. [MASS = 66130

4
4

Homo
sapiens (Human) PROTEIN FAM49B. [MASS = 36748

4
4

Homo
sapiens (Human) 47 KDA HEAT SHOCK PROTEIN PRECURSOR. [MASS = 46267

4
5

Homo
sapiens (Human) PEPTIDYLPROLYL ISOMERASE B PRECURSOR. [MASS = 23743

4
4

Homo
sapiens (Human) ISOFORM 1 OF EXPORTIN-5. [MASS = 136311

4
4

Homo
sapiens (Human) CADHERIN-2 PRECURSOR. [MASS = 99851

4
4

Homo
sapiens (Human) ISOFORM 3 OF DREBRIN-LIKE PROTEIN. [MASS = 49042

4
5

Homo
sapiens (Human) 16 KDA PROTEIN. [MASS = 16122

4
4

Homo
sapiens (Human) PROBABLE ATP-DEPENDENT RNA HELICASE DDX23. [MASS = 95647

4
4

Homo
sapiens (Human) CORONIN-1C. [MASS = 53249

4
4

Homo
sapiens (Human) SIGNAL RECOGNITION PARTICLE 14 KDA PROTEIN. [MASS = 14544

4
4

Homo
sapiens (Human) TROPOMYOSIN 1 ALPHA CHAIN ISOFORM 2. [MASS = 32678

4
4

Homo
sapiens (Human) VON HIPPEL-LINDAU BINDING PROTEIN 1. [MASS = 26535

4
7

Homo
sapiens (Human) ACETYL-COA ACETYLTRANSFERASE, CYTOSOLIC. [MASS = 41351

4
5

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L19. [MASS = 23466

4
8

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S2. [MASS = 31324

4
5

Homo
sapiens (Human) ISOFORM 1 OF MELANOMA-ASSOCIATED ANTIGEN D2. [MASS = 64954

4
7

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L3. [MASS = 45978

4
18

Homo
sapiens (Human) UBIQUITIN AND RIBOSOMAL PROTEIN S27A PRECURSOR.

[MASS = 17965

4
4

Homo
sapiens (Human) PREDICTED: SIMILAR TO RAN-SPECIFIC GTPASE-ACTIVATING

PROTEIN. [MASS = 35024

4
5

Homo
sapiens (Human) SEPTIN 9. [MASS = 63633

4
4

Homo
sapiens (Human) SPERMATID PERINUCLEAR RNA-BINDING PROTEIN. [MASS = 73765

4
5

Homo
sapiens (Human) PROBABLE ATP-DEPENDENT RNA HELICASE DDX46. [MASS = 117461

4
4

Homo
sapiens (Human) RAS-RELATED PROTEIN RAB-14. [MASS = 23766

4
6

Homo
sapiens (Human) MARCKS-RELATED PROTEIN. [MASS = 19398

4
4

Homo
sapiens (Human) GCN1-LIKE PROTEIN 1. [MASS = 292930

4
4

Homo
sapiens (Human) KINESIN LIGHT CHAIN 1 ISOFORM 2. [MASS = 65310

4
4

Homo
sapiens (Human) ISOFORM 2 OF AT-RICH INTERACTIVE DOMAIN-CONTAINING

PROTEIN 1A. [MASS = 218335

4
5

Homo
sapiens (Human) TROPOMYOSIN 4. [MASS = 28522

4
4

Homo
sapiens (Human) EARLY ENDOSOME ANTIGEN 1. [MASS = 162466

4
6

Homo
sapiens (Human) RETICULOCALBIN-1 PRECURSOR. [MASS = 38890

4
4

Homo
sapiens (Human) ISOFORM 1 OF APOPTOSIS INHIBITOR 5. [MASS = 57561

4
4

Homo
sapiens (Human) ISOFORM 1 OF FOCAL ADHESION KINASE 1. [MASS = 119233

4
7

Homo
sapiens (Human) TUBULIN-SPECIFIC CHAPERONE B. [MASS = 27326

4
4

Homo
sapiens (Human) HSPC121. [MASS = 44423

4
6

Homo
sapiens (Human) SEPTIN-11. [MASS = 49267

4
6

Homo
sapiens (Human) NEUROCAN CORE PROTEIN PRECURSOR. [MASS = 142973

4
4

Homo
sapiens (Human) LYSYL-TRNA SYNTHETASE. [MASS = 68048

4
5

Homo
sapiens (Human) 6-PHOSPHOGLUCONOLACTONASE. [MASS = 27547

4
7

Homo
sapiens (Human) SPLICING FACTOR, ARGININE/SERINE-RICH 2. [MASS = 25345

4
4

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L7A. [MASS = 29864

4
6

Homo
sapiens (Human) GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(T) SUBUNIT

BETA 2. [MASS = 37200

4
4

Homo
sapiens (Human) ISOFORM LONG OF 60 KDA SS-A/RO RIBONUCLEOPROTEIN.

[MASS = 60671

4
4

Homo
sapiens (Human) SUPERKILLER VIRALICIDIC ACTIVITY 2-LIKE 2. [MASS = 117805

4
5

Homo
sapiens (Human) ISOFORM LONG OF TRIFUNCTIONAL PURINE BIOSYNTHETIC

PROTEIN ADENOSINE-3. [MASS = 107767

4
4

Homo
sapiens (Human) HEPARIN COFACTOR 2 PRECURSOR. [MASS = 60178

4
5

Homo
sapiens (Human) APOLIPOPROTEIN A-II PRECURSOR. [MASS = 11175

4
4

Homo
sapiens (Human) MYOSIN-11. [MASS = 227339

4
7

Homo
sapiens (Human) HISTONE H1.2. [MASS = 21234

4
8

Homo
sapiens (Human) HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN G. [MASS = 42332

4
5

Homo
sapiens (Human) GLUCOSAMINE-6-PHOSPHATE ISOMERASE. [MASS = 32669

4
4

Homo
sapiens (Human) DNA LIGASE 1. [MASS = 101736

4
4

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L18A. [MASS = 20762

4
5

Homo
sapiens (Human) CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR 73 KDA

SUBUNIT. [MASS = 77486

3
3

Homo
sapiens (Human) PROTEASOME ACTIVATOR COMPLEX SUBUNIT 1. [MASS = 28723

3
3

Homo
sapiens (Human) ACYLAMINO-ACID-RELEASING ENZYME. [MASS = 81225

3
5

Homo
sapiens (Human) 60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL PRECURSOR.

[MASS = 61055

3
3

Homo
sapiens (Human) THO COMPLEX SUBUNIT 4. [MASS = 27558

3
3

Homo
sapiens (Human) SMALL NUCLEAR RIBONUCLEOPROTEIN SM D1. [MASS = 13282

3
3

Homo
sapiens (Human) FRUCTOSE-BISPHOSPHATE ALDOLASE C. [MASS = 39325

3
14

Homo
sapiens (Human) ALPHA-2-HS-GLYCOPROTEIN PRECURSOR. [MASS = 39325

3
4

Homo
sapiens (Human) IMPORTIN ALPHA-4 SUBUNIT. [MASS = 57887

3
5

Homo
sapiens (Human) GTP BINDING PROTEIN 1. [MASS = 72454

3
3

Homo
sapiens (Human) ISOFORM 1 OF DAZ-ASSOCIATED PROTEIN 1. [MASS = 43383

3
3

Homo
sapiens (Human) VACUOLAR PROTEIN SORTING 26A. [MASS = 38170

3
3

Homo
sapiens (Human) CYTOPLASMIC FMR1 INTERACTING PROTEIN 1 ISOFORM A.

[MASS = 145182

3
3

Homo
sapiens (Human) ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL

PRECURSOR. [MASS = 59751

3
4

Homo
sapiens (Human) ACONITATE HYDRATASE, MITOCHONDRIAL PRECURSOR.

[MASS = 85425

3
6

Homo
sapiens (Human) LUNG CANCER ONCOGENE 7. [MASS = 37889

3
4

Homo
sapiens (Human) F-ACTIN CAPPING PROTEIN ALPHA-2 SUBUNIT. [MASS = 32818

3
4

Homo
sapiens (Human) ISOFORM DUT-M OF DEOXYURIDINE 5′-TRIPHOSPHATE

NUCLEOTIDOHYDROLASE, MITOCHONDRIAL PRECURSOR. [MASS = 26706

3
6

Homo
sapiens (Human) ISOFORM 1 OF ALPHA-ADDUCIN. [MASS = 80955

3
3

Homo
sapiens (Human) ISOFORM 1 OF PROTEIN PHOSPHATASE 1 REGULATORY

SUBUNIT 7. [MASS = 41564

3
3

Homo
sapiens (Human) BM-010. [MASS = 36153

3
3

Homo
sapiens (Human) PROTEIN TYROSINE PHOSPHATASE,-RECEPTOR-TYPE, ZETA1

PRECURSOR. [MASS = 254587

3
3

Homo
sapiens (Human) ISOFORM 3 OF UDP-N-ACETYLGLUCOSAMINE-PEPTIDE N-

ACETYLGLUCOSAMINYLTRANSFERASE 110 KDA SUBUNIT. [MASS = 116925

3
3

Homo
sapiens (Human) HIV TAT SPECIFIC FACTOR 1. [MASS = 85853

3
4

Homo
sapiens (Human) UV EXCISION REPAIR PROTEIN RAD23 HOMOLOG B.

[MASS = 43171

3
3

Homo
sapiens (Human) PNAS-125. [MASS = 23755

3
5

Homo
sapiens (Human) IGKV1-5 PROTEIN. [MASS = 26234

3
19

Homo
sapiens (Human) HEMOGLOBIN SUBUNIT GAMMA-1. [MASS = 16009

3
3

Homo
sapiens (Human) EXOSOME COMPLEX EXONUCLEASE RRP42. [MASS = 31835

3
3

Homo
sapiens (Human) ISOFORM 2 OF TRANSCRIPTION FACTOR BTF3. [MASS = 17699

3
4

Homo
sapiens (Human) HISTONE-BINDING PROTEIN RBBP4. [MASS = 47525

3
3

Homo
sapiens (Human) SERINE/THREONINE-PROTEIN KINASE MRCK BETA.

[MASS = 194315

3
3

Homo
sapiens (Human) HYPOTHETICAL PROTEIN DKFZP68610180 (FRAGMENT).

[MASS = 28810

3
4

Homo
sapiens (Human) THYMOPOIETIN ISOFORM BETA. [MASS = 50670

3
3

Homo
sapiens (Human) DNA-DIRECTED RNA POLYMERASE II LARGEST SUBUNIT.

[MASS = 217206

3
5

Homo
sapiens (Human) FACTOR VII ACTIVE SITE MUTANT IMMUNOCONJUGATE.

[MASS = 75553

3
3

Homo
sapiens (Human) TUBULIN-SPECIFIC CHAPERONE A. [MASS = 12724

3
3

Homo
sapiens (Human) TRYPTOPHANYL-TRNA SYNTHETASE. [MASS = 53165

3
3

Homo
sapiens (Human) ISOFORM 1 OF SYMPLEKIN. [MASS = 141148

3
3

Homo
sapiens (Human) SERINE/THREONINE-PROTEIN PHOSPHATASE 4 CATALYTIC

SUBUNIT. [MASS = 35080

3
3

Homo
sapiens (Human) ANTITHROMBIN III VARIANT. [MASS = 52692

3
7

Homo
sapiens (Human) UBIQUITIN-ACTIVATING ENZYME E1. [MASS = 25052

3
3

Homo
sapiens (Human) PREDICTED: SIMILAR TO CHLORIDE INTRACELLULAR CHANNEL

PROTEIN 4. [MASS = 17863

3
3

Homo
sapiens (Human) ISOFORM 1 OF TRANSCRIPTION ELONGATION FACTOR SPT5.

[MASS = 121000

3
4

Homo
sapiens (Human) EUKARYOTIC INITIATION FACTOR 5A ISOFORM I VARIANT A.

[MASS = 20170

3
3

Homo
sapiens (Human) ISOFORM 3 OF DNA REPAIR PROTEIN RAD50. [MASS = 138432

3
3

Homo
sapiens (Human) COP9 SIGNALOSOME COMPLEX SUBUNIT 5. [MASS = 37448

3
3

Homo
sapiens (Human) HYPOTHETICAL PROTEIN DKFZP547J2313. [MASS = 18829

3
4

Homo
sapiens (Human) CORTICOSTEROID-BINDING GLOBULIN PRECURSOR. [MASS = 45141

3
4

Homo
sapiens (Human) PROFILIN 2 ISOFORM A. [MASS = 15046

3
4

Homo
sapiens (Human) ISOFORM GAMMA-1 OF SERINE/THREONINE-PROTEIN PHOSPHATASE

PP1-GAMMA CATALYTIC SUBUNIT. [MASS = 36984

3
4

Homo
sapiens (Human) ISOFORM 1 OF UBIQUITIN-PROTEIN LIGASE BRE1B. [MASS = 113678

3
3

Homo
sapiens (Human) ISOFORM 1 OF RAS GTPASE-ACTIVATING PROTEIN 1. [MASS = 116403

3
5

Homo
sapiens (Human) ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1A. [MASS = 41569

3
3

Homo
sapiens (Human) CADHERIN-5 PRECURSOR. [MASS = 87516

3
3

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S14. [MASS = 16142

3
3

Homo
sapiens (Human) LETHAL GIANT LARVAE HOMOLOG 1. [MASS = 115388

3
3

Homo
sapiens (Human) LEUCINE ZIPPER TRANSCRIPTION FACTOR-LIKE 1. [MASS = 34592

3
4

Homo
sapiens (Human) MEPRIN A SUBUNIT ALPHA PRECURSOR. [MASS = 84368

3
4

Homo
sapiens (Human) WD REPEAT PROTEIN 61. [MASS = 33581

3
3

Homo
sapiens (Human) TRIPARTITE MOTIF-CONTAINING PROTEIN 2. [MASS = 81530

3
4

Homo
sapiens (Human) ISOFORM 1 OF ATP-DEPENDENT RNA HELICASE DDX19B.

[MASS = 53927

3
3

Homo
sapiens (Human) SORTING NEXIN 1 ISOFORM C. [MASS = 53304

3
3

Homo
sapiens (Human) HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT.

[MASS = 49513

3
3

Homo
sapiens (Human) SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT

REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5. [MASS = 121905

3
3

Homo
sapiens (Human) HEAT SHOCK 70 KDA PROTEIN 4L. [MASS = 94486

3
4

Homo
sapiens (Human) AFLATOXIN B1 ALDEHYDE REDUCTASE MEMBER 2. [MASS = 39589

3
4

Homo
sapiens (Human) D-DOPACHROME DECARBOXYLASE. [MASS = 12581

3
3

Homo
sapiens (Human) THIMET OLIGOPEPTIDASE. [MASS = 78709

3
3

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L38. [MASS = 8087

3
3

Homo
sapiens (Human) ALDEHYDE DEHYDROGENASE 16 FAMILY, MEMBER A1.

[MASS = 85127

3
3

Homo
sapiens (Human) CYTOCHROME B5 REDUCTASE ISOFORM 1. [MASS = 34235

3
3

Homo
sapiens (Human) NETRIN RECEPTOR DCC PRECURSOR. [MASS = 158457

3
4

Homo
sapiens (Human) ISOPENTENYL-DIPHOSPHATE DELTA ISOMERASE. [MASS = 32485

3
4

Homo
sapiens (Human) PHOSPHATIDYLINOSITOL TRANSFER PROTEIN, BETA.

[MASS = 31540

3
3

Homo
sapiens (Human) HIGH MOBILITY GROUP PROTEIN B2. [MASS = 23903

3
5

Homo
sapiens (Human) TUMOR PROTEIN, TRANSLATIONALLY-CONTROLLED 1.

[MASS = 21526

3
3

Homo
sapiens (Human) BLEOMYCIN HYDROLASE. [MASS = 52562

3
9

Homo
sapiens (Human) ALPHA-ENOLASE, LUNG SPECIFIC. [MASS = 49477

3
4

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L12. [MASS = 17819

3
3

Homo
sapiens (Human) CELL DIVISION CYCLE 5-LIKE PROTEIN. [MASS = 92251

3
3

Homo
sapiens (Human) ISOFORM 2 OF UBIQUITIN CARBOXYL-TERMINAL

HYDROLASE 47. [MASS = 147180

3
3

Homo
sapiens (Human) COMPLEMENT C5 PRECURSOR. [MASS = 188331

3
3

Homo
sapiens (Human) ISOFORM 1 OF CYTOPLASMIC LINKER PROTEIN 2. [MASS = 115837

3
3

Homo
sapiens (Human) THIOREDOXIN REDUCTASE 1, CYTOPLASMIC PRECURSOR.

[MASS = 54707

3
4

Homo
sapiens (Human) HISTONE H1X. [MASS = 22487

3
3

Homo
sapiens (Human) ISOFORM GTBP-N OF DNA MISMATCH REPAIR PROTEIN

MSH6. NASS = 152786

3
3

Homo
sapiens (Human) BILIVERDIN REDUCTASE A PRECURSOR. [MASS = 33428

3
3

Homo
sapiens (Human) LAMINA-ASSOCIATED POLYPEPTIDE 2 ISOFORM ALPHA.

[MASS = 75361

3
3

Homo
sapiens (Human) SYNAPTIC VESICLE MEMBRANE PROTEIN VAT-1

HOMOLOG. [MASS = 41920

3
3

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L8. [MASS = 27893

3
3

Homo
sapiens (Human) PHYTANOYL-COA HYDROXYLASE INTERACTING

PROTEIN-LIKE. [MASS = 42486

3
3

Homo
sapiens (Human) CONDENSIN COMPLEX SUBUNIT 2. [MASS = 82535

3
3

Homo
sapiens (Human) HIGH-MOBILITY GROUP BOX 1. [MASS = 15185

3
3

Homo
sapiens (Human) SELENIDE, WATER DIKINASE 1. [MASS = 42911

3
5

Homo
sapiens (Human) APOLIPOPROTEIN M. [MASS = 21253

3
3

Homo
sapiens (Human) HYPOTHETICAL PROTEIN DKFZP686M09245. [MASS = 61598

3
3

Homo
sapiens (Human.) ISOFORM 1 OF EXOSOME COMPLEX EXONUCLEASE RRP44.

[MASS = 109003

3
3

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L10A. [MASS = 24700

3
3

Homo
sapiens (Human) ECHINODERM MICROTUBULE-ASSOCIATED.PROTEIN-LIKE 4.

[MASS = 108903

3
3

Homo
sapiens (Human) CALPAIN-1 CATALYTIC SUBUNIT. [MASS = 81890

3
4

Homo
sapiens (Human) 55 KDA PROTEIN. [MASS = 55183

3
3

Homo
sapiens (Human) ZYXIN. [MASS = 67285

3
5

Homo
sapiens (Human) DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 1.

[MASS = 40542

3
3

Homo
sapiens (Human) TYROSYL-TRNA SYNTHETASE, CYTOPLASMIC. [MASS = 59012

3
3

Homo
sapiens (Human) PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 2.

[MASS = 79555

3
3

Homo
sapiens (Human) U4/U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 1. [MASS = 90255

3
3

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S21. [MASS = 9111

3
3

Homo
sapiens (Human) DNAJ HOMOLOG SUBFAMILY C MEMBER 7. [MASS = 56441

3
4

Homo
sapiens (Human) CALSYNTENIN 1 ISOFORM 2. [MASS = 108643

3
3

Homo
sapiens (Human) TWINFILIN ISOFORM 1. [MASS = 43918

3
3

Homo
sapiens (Human) CENTROSOMAL PROTEIN 170 KDA ISOFORM ALPHA.

[MASS = 175436

3
3

Homo
sapiens (Human) ISOFORM 1 OF 26S PROTEASE REGULATORY SUBUNIT 6B.

[MASS = 47366

3
4

Homo
sapiens (Human) U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A. [MASS = 31148

3
4

Homo
sapiens (Human) PEPTIDYL-PROLYL CIS-TRANS ISOMERASE A. [MASS = 17881

3
3

Homo
sapiens (Human) PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 2.

[MASS = 79555

3
3

Homo
sapiens (Human) RAS-RELATED PROTEIN RAB-5C. [MASS = 23483

3
3

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S8. [MASS = 24074

3
6

Homo
sapiens (Human) PERIPHERIN. [MASS = 53878

3
3

Homo
sapiens (Human) NUCLEOSIDE DIPHOSPHATE KINASE B. [MASS = 17298

3
5

Homo
sapiens (Human) SEC31L1 PROTEIN. [MASS = 121651

3
3

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S16. [MASS = 16314

3
3

Homo
sapiens (Human) UROPORPHYRINOGEN DECARBOXYLASE. [MASS = 40787

3
3

Homo
sapiens (Human) 114 KDA PROTEIN. [MASS = 113977

3
4

Homo
sapiens (Human) COLLAGEN ALPHA-1(V) CHAIN PRECURSOR. [MASS = 183560

3
3

Homo
sapiens (Human) CYTOPLASMIC DYNEIN 1 LIGHT INTERMEDIATE CHAIN 2.

[MASS = 54099

3
3

Homo
sapiens (Human) CYTOSOLIC AMINOPEPTIDASE P. [MASS = 74798

3
3

Homo
sapiens (Human) ISOFORM 2 OF SPLICING FACTOR 1. [MASS = 68502

3
3

Homo
sapiens (Human) PREDICTED: SIMILAR TO BASIC LEUCINE ZIPPER AND W2

DOMAINS 1. [MASS = 34090

3
3

Homo
sapiens (Human) ISOFORM 1 OF URIDINE 5′-MONOPHOSPHATE SYNTHASE.

[MASS = 52222

3
3

Homo
sapiens (Human) DEAD BOX POLYPEPTIDE 42 PROTEIN. [MASS = 102975

3
4

Homo
sapiens (Human) ISOFORM C OF NEURAL CELL ADHESION MOLECULE 1, 120

KDA ISOFORM PRECURSOR. [MASS = 83985

3
3

Homo
sapiens (Human) COLD-INDUCIBLE RNA-BINDING PROTEIN. [MASS = 18648

3
4

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L18. [MASS = 21503

3
3

Homo
sapiens (Human) ISOFORM 1 OF JMJC DOMAIN-CONTAINING.HISTONE

DEMETHYLATION PROTEIN 2B. [MASS = 191611

3
3

Homo
sapiens (Human) RCC1 PROTEIN. [MASS = 48146

3
3

Homo
sapiens (Human) LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47. [MASS = 63473

3
3

Homo
sapiens (Human) ISOFORM 1 OF RNA-BINDING PROTEIN NOVA-1. [MASS = 52056

3
6

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S10. [MASS = 18898

3
3

Homo
sapiens (Human) SPLICING FACTOR U2AF 65 KDA SUBUNIT. [MASS = 53501

3
3

Homo
sapiens (Human) ISOFORM SHORT OF PROTEASOME SUBUNIT ALPHA TYPE 1.

[MASS = 29556

3
3

Homo
sapiens (Human) ALPHA-1-ACID GLYCOPROTEIN 2 PRECURSOR. [MASS = 23603

3
4

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S23. [MASS = 15676

3
3

Homo
sapiens (Human) DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN

GLYCOSYLTRANSFERASE 67 KDA SUBUNIT PRECURSOR. [MASS = 72778

3
3

Homo
sapiens (Human) HYPOTHETICAL PROTEIN LOC387104. [MASS = 103199

3
3

Homo
sapiens (Human) PROTEIN TRANSPORT PROTEIN SEC24C. [MASS = 118315

3
4

Homo
sapiens (Human) THIOREDOXIN. [MASS = 11606

3
3

Homo
sapiens (Human) CYTOSOLIC PURINE 5′-NUCLEOTIDASE. [MASS = 64970

3
6

Homo
sapiens (Human) PREDICTED: SIMILAR TO RIBOSOMAL PROTEIN S3A ISOFORM 1.

[MASS = 24821

3
4

Homo
sapiens (Human) ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32

FAMILY MEMBER A. [MASS = 28585

3
3

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S25. [MASS = 13742

3
3

Homo
sapiens (Human) ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 3. [MASS = 20456

3
4

Homo
sapiens (Human) CYSTATIN B. [MASS = 11140

3
3

Homo
sapiens (Human) PHOSPHOGLUCOMUTASE-2-LIKE 1. [MASS = 70456

3
3

Homo
sapiens (Human) 40S RIBOSOMAL PROTEIN S9. [MASS = 22460

3
3

Homo
sapiens (Human) ANKYRIN REPEAT AND FYVE DOMAIN CONTAINING 1

ISOFORM 1. [MASS = 128486

3
3

Homo
sapiens (Human) ISOFORM LONG OF COLD SHOCK DOMAIN-CONTAINING

PROTEIN E1. [MASS = 88885

3
3

Homo
sapiens (Human) DEAD (ASP-GLU-ALA-ASP) BOX POLYPEPTIDE 39, ISOFORM 2.

[MASS = 35095

3
3

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L23A. [MASS = 17695

3
4

Homo
sapiens (Human) ALPHA-INTERNEXIN. [MASS = 55391

3
3

Homo
sapiens (Human) ISOFORM 3 OF ANAMORSIN. [MASS = 32213

3
3

Homo
sapiens (Human) SPLICING FACTOR, ARGININE/SERINE-RICH 4. [MASS = 56678

3
3

Homo
sapiens (Human) DEBRANCHING ENZYME HOMOLOG 1. [MASS = 61555

3
3

Homo
sapiens (Human) ISOFORM 2 OF PROTEASOME SUBUNIT ALPHA TYPE 3. [MASS = 27516

3
3

Homo
sapiens (Human) MICROSOMAL TRIGLYCERIDE TRANSFER PROTEIN LARGE SUBUNIT

PRECURSOR. [MASS = 99351

3
4

Homo
sapiens (Human) ISOFORM B OF NEURONAL-SPECIFIC SEPTIN-3. [MASS = 40100

3
4

Homo
sapiens (Human) 60S RIBOSOMAL PROTEIN L28. [MASS = 15616

3
6

Homo
sapiens (Human) PREDICTED: SIMILAR TO HETEROGENEOUS NUCLEAR

RIBONUCLEOPROTEIN K ISOFORM A ISOFORM 2. [MASS = 24258

Patent
Attorney Docket No. 01948/144WO2
Characterization of the Rat Embryonic Proteome

CSF was collected from the lateral ventricle of E12.5, E14.5, and E17.5 rat embryos and from the fourth ventricle of E14.5 rat embryos. CSF from two litters (approximately 20-24 rat embryos) was pooled for each time point and was separated by 1-D SDS-PAGE and the proteins were visualized with Coomassie blue stain. FIG. 1C shows the Coomassie stained protein pattern of CSF collected from all three time-points. Mass spectrometry analysis of the rat CSF was performed separately for E12.5, E14.5, E17.5 lateral ventricle, and E14.5 fourth ventricle and presented as Supplementary information table 4. There were 423 proteins identified in E12.5 LV CSF, 318 proteins in E14.5 LV, 249 proteins in E14.5 4^thV, and 382 proteins in E17.5 LV. There are 137 proteins common to E12.5, E14.5, and E17.5 rat CSF samples that are presented in Table 3, which includes the name of the protein, its molecular weight, subcellular localization, function, tissue specificity. Also included are relevant notes about each protein. Interestingly, there are 61 proteins identified in E12.5 LV, E14.5 LV, and E17.5 LV that were not identified in E14.5 4^thV and only 5 proteins identified in E12.5 LV, E14.5 4^thV, and E17.5 LV that were not identified in E14.5 LV. This does not appear to be simply due to an overall reduction in E14.5^thV protein concentration as similar numbers of peptides were identified for the proteins found in common with LV CSF samples. Instead, the difference suggests potential differences in the protein composition of CSF between the lateral and fourth ventricles, though further studies would be needed to confirm this and to assess its significance.

TABLE 3

Common proteins from mass spectrometry analysis of embryonic rat CSF isolated from E12.5 LV, E14.5

LV and 4^thV, and E17.5 LV. The number of peptides is listed from E14.5 4^thV.

# of

unique

peptides
Total #

from
of
Accession

Subcellular

protein
peptides
Number
Name of Protein
MW
location
Function
Tissue specificity
Notes

120
617
Q7TMA5
AA1064 - apolipoprotein B
536024
Secreted
Lipid and fatty acid
Plasma
Apo B-100 functions as a

transport and metabolism

recognition signal for the

cellular binding and

internalization of LDL

particles by the apoB/E

receptor

66
382
Q4G047
Apolipoprotein B - fragment
165356
Secreted
Lipid and fatty acid
Plasma
Fragment molecule

transport and metabolism

37
135
P04937
SPLICE ISOFORM 1 OF FIBRONECTIN
272511
Secreted,
Cell adhesion mediated
Plasma
Integrin signaling

PRECURSOR

extracellular
signaling
fibronectin made
pathway

space,

by liver and

extracellular

cellular

matrix

fibronectin made

by fibroblasts,

epithelial and

other cell types is

deposited in the

extracellular

matrix

34
365
P02773
SPLICE ISOFORM 1 OF ALPHA-FETOPROTEIN
68386
Secreted
Transport/Transfer-Carrier
Plasma
Binds copper, nickel, and

PRECURSOR

fatty acids as well as, and

bilirubin less well than,

serum albumin.

31
120
P02651
APOLIPOPROTEIN A-IV PRECURSOR
44456
Secreted
Lipid and fatty acid
Plasma
May have a role in

transport and metabolism

chylomicrons and VLDL

secretion and catabolism.

Apoa-IV is a major

component of HDL and

chylomicrons.

28
53
P06238
ALPHA-2-MACROGLOBULIN PRECURSOR
163701
Secreted
Serine Protease Inhibitor
Plasma
Plays role in acute phase

response

21
26
Q9JL97
GPI-ANCHORED CERULOPLASMIN
123749
Cell Membrane
Transport, Transfer/Carrier,
Brain
Metal ion binding

Oxidoreductase

oxidoreductase activity

ion transport

21
35
P14046
ALPHA-1-INHIBITOR 3 PRECURSOR
163773
Secreted
Serine Protease Inhibitor
Plasma
Belongs to the alpha-2-

macroglobulin family

involved in inflammtory

response

20
96
Q7TP24
BA1-667 - Transferrin
107448
Secreted
Transport, Transfer/Carrier
Plasma
Iron ion homeostasis,

iron ion transport

19
30
P22063
CONTACTIN-2 PRECURSOR
113043
Cell Membrane
Cell Adhesion -
In neural tissues
May play a role in the

Neurogenesis
in embryos, and
initial growth and

in adult brain,
guidance of axons.

spinal cord and
Belongs to the

cerebellum.
immunoglobulin

superfamily.

18
79
P04639
APOLIPOPROTEIN A-I PRECURSOR
30088
Secreted
Lipid and fatty acid
Major protein of
Participates in the

transport and metabolism
plasma HDL, also
reverse transport of

found in
cholesterol from tissues

chylomicrons.
to the liver for excretion

by promoting cholesterol

efflux from tissues and by

acting as a cofactor for

the lecithin cholesterol

acyltransferase (LCAT).

18
97
P02770
SERUM ALBUMIN PRECURSOR
68719
Secreted
Transport, Transfer/Carrier
Plasma
The main protein of

plasma, has a good

binding capacity for

water, Ca(2+), Na(+),

K(+), fatty acids,

hormones, bilirubin and

drugs. Its main function is

the regulation of the

colloidal osmotic

pressure of blood.

18
33
P08460
PREDICTED: NIDOGEN
138365
Secreted,
Cell - extracellular matrix
Wide distribution
Found in basement

extracellular
adhesion

membranes often

space,

associated with laminin.

extracellular

matrix

15
34
P17475
ALPHA-1-ANTIPROTEINASE PRECURSOR
46136
Secreted
Serine Protease Inhibitor
Plasma
Blood coagulation. The

primary target is elastase,

but also has a moderate

affinity for plasmin and

thrombin.

15
17
Q7TP05
DA1-24 - Complement Factor B
124379
Secreted
Serine Protease -
Plasma
Hydrolase, Peptidase

Complement Mediated

Immunity

14
23
GeneID: 307618
PREDICTED: SIMILAR TO CADHERIN-5
135230
Cell junction,
Cell Adhesion
Endothelial
Cell - Cell interactions

cell membrane

tissues and brain
and juntions

13
20
Q80ZA3
ALPHA-2 ANTIPLASMIN
46465
Secreted
Serine Protease Inhibitor
Plasma
Blood coagulation

13
18
P25304
SPLICE ISOFORM 1 OF AGRIN PRECURSOR
208646
Secreted,
Cell adhesion mediated
Embryonic
Component of the basal

extracellular
signaling
nervous system
lamina that binds to

space,

and muscle
laminin.

extracellular

matrix

13
25
GeneID: 313641
PREDICTED: SIMILAR TO HEPARAN SULFATE
377284
Secreted,
Cell adhesion mediated
Widely
Integral component of

PROTEOGLYCAN 2 (Perlecan)

extracellular
signaling
distrubuted
basement membranes

space,

extracellular

matrix

13
19
O35802
INTER-ALPHA-INHIBITOR H4 HEAVY CHAIN
103755
Secreted
Serine Protease Inhibitor
Plasma
Involved in acute phase

response

12
14
Q68FP1
GELSOLIN
86286
Secreted and
Cell structure
Ubiquitous
Actin binding protein,

Cytoplasm

may be involved in

myelination

12
15
Q5M7T5
SERINE/CYSTEINE PROTEINASE INHIBITOR,
52234
Secreted
Protease inhibitor with a
Plasma
Belongs to the serpin

CLADE C, MEMBER 1

wide spectrum of protein

family

targets

12
22
P01026
COMPLEMENT C3 PRECURSOR
186460
Secreted
Complement mediated
Plasma
Plays a central role in the

immunity

activation of the

complement system.

12
24
P02650
APOLIPOPROTEIN E PRECURSOR
35753
Secreted
Lipid and fatty acid
Plasma
Mediates the binding,

transport and metabolism

internalization, and

catabolism of lipoprotein

particles

11
14
Q62917
LAR RECEPTOR-LINKED TYROSINE
181130
Cell Membrane
Cell adhesion mediated
Widely
May play a role in neurite

PHOSPHATASE

signaling, cell structure and
distrubuted
outgrowth.

motility

11
12
Q63155
DELETED IN COLORECTAL CANCER
158142
Membrane -
Ligand mediated signaling,
During
Netrin receptor activity,

Intracellular
cell adhesion
development
transcription coactivator

expressed highly
activity, axon guidance,

in brain and
and apoptosis

neural tube

11
12
NM_199093
SERINE PEPTIDASE INHIBITOR, CLADE G,
55611
Secreted
Protease inhibitor
Plasma
Involved in the regulation

MEMBER 1

of classical component

pathway

11
17
P31211
CORTICOSTEROID-BINDING GLOBULIN
44672
Secreted
Transpost and Serine
Expressed by the
Major transport protein

PRECURSOR

Protease Inhibitor
liver; secreted in
for glucocorticoids and

plasma.
progestins in the blood

11
12
GeneID: 298941
PREDICTED: SIMILAR TO LAMININ B1
228429
Secreted,
Extracellular matrix linker
Widely distributed
Is thought to mediate the

extracellular
protein-mediated signaling
in basement
attachment, migration

space,

membranes
and organization of cells

extracellular

into tissues during

matrix

embryonic development

by interacting with other

extracellular matrix

components.

11
11
P05197
ELONGATION FACTOR 2
95153
Cytoplasm -
Protein biosynthesis -
Ubiquitous
Promotes translocation of

Intracellular
Translational elongation

protein chain from A site

factor

to P site on ribosome

11
14
P06399
SPLICE ISOFORM 1 OF FIBRINOGEN ALPHA
86686
Secreted
Blood clotting
Plasma
Monomers polymerize

CHAIN PRECURSOR

into fibrin and acts as a

cofactor in platelet

aggregation

10
21
Q9Z1Y3
NEURAL-CADHERIN PRECURSOR
99686
Cell Membrane
Cell adhesion
Testis and
May be involved in

neurons
neuronal tissue

recognition

10
22
GeneID: 315191
PREDICTED: SIMILAR TO FIBULIN-1
78072
Secreted,
Cell adhesion mediated
Detected in most
Incorporated into

PRECURSOR

extracellular
signaling
organs (brain,
fibronectin-containing

space,

heart, lung,
matrix fibers. May play a

extracellular

spleen, liver and
role in cell adhesion and

matrix

kidney). Neurons
migration along protein

are the
fibers within the

predominant
extracellular matrix

source of
(ECM). Could be

production in the
important for certain

brain. Not
developmental processes

expressed
and contribute to the

significantly by
supramolecular

astrocytes or
organization of ECM

microglia.
architecture, in particular

to those of basement

membranes.

10
11
Q64610
ECTONUCLEOTIDE
101310
Membrane
Hydrolase
Abundantly
Hydrolytically removes 5′-

PYROPHOSPHATASE/PHOSPHODIESTERASE 2

expressed in
nucleotides successively

cerebrum and
from the 3′-hydroxy

cerebellum.
termini of 3′-hydroxy-

Localized in
terminated

secretory
oligonucleotides

epithelial cells in

the brain and the

eye including

choroid plexus

epithelial cells,

ciliary epithelial

cells, iris pigment

epithelial cells,

and retinal

pigment cells.

10
21
P02454
COLLAGEN ALPHA-1(I) CHAIN PRECURSOR
137886
Secreted,
Cell adhesion - Cell
Forms the fibrils
Component of connective

extracellular
structure
of tendon,
tissue

space,

ligaments and

extracellular

bones

matrix

10
21
P51886
LUMICAN PRECURSOR
38279
Secreted,
Cell adhesion mediated
Widely distributed
Binds to laminin

extracellular
signaling

space,

extracellular

matrix

9
51
P02767
TRANSTHYRETIN PRECURSOR
15720
Secreted
Hormone transport
Most abundant in
Thyroid hormone-binding

the choroid
protein. Probably

plexus. Also
transports thyroxine from

present in the
the bloodstream to the

liver
brain

9
13
P00787
CATHEPSIN B PRECURSOR
37470
Lysosome
Cysteine protease
Widely distributed
Participates in

intracellular degradation

and turnover of proteins.

Has been implicated in

tumor invasion and

metastasis

9
13
Q03626
ALPHA(1)-INHIBITOR 3, VARIANT I
165326
Secreted
Serine Protease Inhibitor
Serum
Invovled in acute phase

PRECURSOR

inflammatory response

9
11
GeneID: 116669
PREDICTED: VON WILLEBRAND FACTOR
308474
Secreted,
Cell adhesion - Blood
Plasma
Major coagulation factor

extracellular
clotting

space,

extracellular

matrix

9
18
Q5M7V3
LOC367586 PROTEIN - Immunoglobulin Gamma
50949
Cell Membrane
B-cell and antibody-
Plasma
Associates non-

heavy Chain

mediated immunity

covalently with beta-2-

microglobulin, antigen

binding

8
10
Q91XW0
HEAT SHOCK PROTEIN 86
84815
Cytoplasm
Molecular chaperone,
Ubiquitous

protein folding, stress

response

8
8
Q920H8
HEPHAESTIN PRECURSOR
129593
Membrane
Oxidase
Expressed highly
May function as a

in intestine, lung
ferroxidase for ferrous (II)

and brain
to ferric ion (III)

conversion and in copper

transport and

homeostasis

8
13
P13941
COLLAGEN ALPHA-1(III) CHAIN PRECURSOR
138936
Secreted,
Cell adhesion - Cell
Widely
Component of connective

extracellular
structure
distributed, highly
tissue

space,

expressed in

extracellular

colon and blood

matrix

vessels

8
9
P58751
SPLICE ISOFORM 1 OF REELIN PRECURSOR
387531
Secreted,
Serine protease
Brain
Plays a role in layering of

extracellular

neurons in the cerebral

space,

cortex and cerebellum.

extracellular

Regulates microtubule

matrix

function in neurons and

neuronal migration.

Affects migration of

sympathetic

preganglionic neurons in

the spinal cord, where it

seems to act as a barrier

to neuronal migration

8
20
Q01177
PLASMINOGEN PRECURSOR
90536
Secreted
Protease
Plasma
Plasmin dissolves the

fibrin of blood clots and

acts as a proteolytic

factor in a variety of other

processes including

embryonic development,

tissue remodeling, tumor

invasion, and

inflammation

8
12
O88752
EPSILON 1 GLOBIN
16105
Secreted
Oxygen Transport, Transfer -
Blood
Involved in oxygen

Carrier Protein

transport from the lung to

the various peripheral

tissues

8
13
P08934
SPLICE ISOFORM HMW OF KININOGEN-1
70933
Secreted
Cysteine protease inhibitor
Plasma
Bradykinin is released

PRECURSOR

from kininogen by plasma

kallikrein

8
19
P02680
SPLICE ISOFORM GAMMA-B OF FIBRINOGEN
50633
Secreted
Blood clotting
Plasma
Monomers polymerize

GAMMA CHAIN PRECURSOR

into fibrin and also acts

as a cofactor in platelet

aggregation

8
9
P55280
CADHERIN-6 PRECURSOR
88341
Cell Membrane
Cell adhesion
Highly expressed
Also known as Kidney

in kidney and
cadherin

brain

7
9
P69897
TUBULIN BETA-5 CHAIN
49671
Intracellular
Cell structure, cell mobility,
Ubiquitously
Tubulin is the major

intracellular protein traffic
expressed with
constituent of

highest levels in
microtubules

spleen, thymus

and immature

brain

7
8
P01015
ANGIOTENSINOGEN PRECURSOR
51982
Secreted
Serine Protease Inhibitor
Expressed by the
Helps regulate volume

liver and secreted
and mineral balance of

in plasma
body fluids

7
8
P24054
SPARC-LIKE PROTEIN 1 PRECURSOR
70634
Secreted,
Matrix glycoprotein Sc1
Expressed in
Function unknown,

extracellular

many types of
believed to bind calcium

space,

neurons in the
and play a role in brain

extracellular

brain
development

matrix

7
7
P97603
156 KDA PROTEIN - Neogenin precursor
156144
Membrane
Cell adhesion
Widely expressed
May be involved as a

regulatory protein in the

transition of

undifferentiated

proliferating cells to their

differentiated state.

Belongs to the

immunoglobulin

superfamily.

7
9
P68136
ACTIN, ALPHA SKELETAL MUSCLE
42051
Cytoplasm
Cell structure, cell motility
Muscle
Actins are highly

conserved proteins that

are involved in various

types of cell motility and

are ubiquitously

expressed in all

eukaryotic cells.

7
9
P62630
ELONGATION FACTOR 1-ALPHA 1
50114
Cytoplasm
Protein biosynthesis,
Ubiquitous
Links tRNA to ribosome

translation regulation

during protein synthesis

7
7
P46462
TRANSITIONAL ENDOPLASMIC RETICULUM
89534
Intracellular
Protein targeting and
Ubiquitous
Necessary for the

ATPASE

localization, intracellular

fragmentation of Golgi

protein traffic

stacks during mitosis and

for their reassembly after

mitosis. Involved in the

formation of the

transitional endoplasmic

reticulum (tER).

7
11
P02466
COLLAGEN ALPHA-2(I) CHAIN PRECURSOR
129564
Secreted,
Cell adhesion - Cell
Forms the fibrils
Involved in skeletal

extracellular
structure
of tendon,
development

space,

ligaments and

extracellular

bones

matrix

7
7
P28494
ALPHA-MANNOSIDASE 2
131242
Golgi apparatus -
Glycosidase
All tissues, mostly
Involved in N-

Intracellular

in adrenal and
glycosylation

thymus

7
9
Q99J86
SPLICE ISOFORM 1 OF ATTRACTIN
163296
Cell membrane
Cell adhesion - Immune
Widely
Involved in the initial

PRECURSOR

defense
distributed, highly
immune cell clustering

expressed in
during Inflammatory

colon and brain
response and may

regulate chemotactic

activity of chemokines.

Has a critical role in

normal myelination in the

central nervous system,

and enhancing cell

survival against oxidative

stress.

6
9
P68370
TUBULIN ALPHA-1 CHAIN
50136
Intracellular
Cell structure, cell mobility,
Widely distributed
Tubulin is the major

chromosome segregation,

constituent of

intracellular protein traffic

microtubules.

6
8
P97686
SPLICE ISOFORM 1 OF NEURONAL CELL
133912
Cell membrane
Cell adhesion
Widely
Ankyrin-binding protein

ADHESION MOLECULE PRECURSOR

distributed, highly
involved in neuron-

expressed in
neuron adhesion. May

brain
play a role in the

molecular assembly of

the nodes of Ranvier

6
8
Q91V15
ALPHA-2-GLOBIN CHAIN
15285
Secreted
Oxygen Transport, Transfer -
Blood
Involved in oxygen

Carrier Protein

transport from the lung to

the various peripheral

tissues

6
6
Q9WVR2
ARCADLIN
103800
Cell Membrane
Cell adhesion
Brain
Neural activity-regulated

cadherin may be involved

in long term potentiation

6
9
Q62657
TENASCIN (FRAGMENT)
62473
Secreted,
Cell adhesion
Widely
Negative regulation of

extracellular

distrubuted
cell adhesion

space,

extracellular

matrix

6
12
P04916
PREDICTED: RETINOL BINDING PROTEIN 4,
23220
Secreted
Vitamin/Co-factor transport -
Plasma
Delivers retinol from the

PLASMA

retinol binding, transporter

liver stores to the

activity

peripheral tissues. In

plasma, the RBP-retinol

complex interacts with

transthyretin, this

prevents its loss by

filtration through the

kidney glomeruli.

6
10
GeneID: 313717
PREDICTED: CALSYNTENIN 1
109351
Cell Membrane
Cell adhesion
Widely distributed
May modulate calcium-

mediated postsynaptic

signals

6
8
GeneID: 302248
PREDICTED: NIDOGEN 2
173960
Secreted,
Cell adhesion, Cell -
Widely distributed
Sulfated glycoprotein

extracellular
extracellular matrix
in basement
widely distributed in

space,
interaction
membranes
basement membranes

extracellular

and tightly associated

matrix,

with laminin. Also binds

basement

to collagen IV and

membrane

perlecan. It probably has

a role in cell-extracellular

matrix interactions

6
7
P11980
PREDICTED: SIMILAR TO PYRUVATE KINASE
57731
Intracellular
Carbohydrate degradation,
Widely distributed
ATP + pyruvate = ADP +

(EC 2.7.1.40) ISOZYME M2 - RAT

glycolysis

phosphoenolpyruvate

6
7
Q63581
T-KININOGEN
47618
Secreted
Cysteine protease inhibitor
Plasma
Plays a role in blood

clotting and regulation of

vasoconstriction and

dilation

6
10
GeneID: 84407
PREDICTED: CADHERIN 11
88036
Cell Membrane
Cell adhesion
Widely distributed
Involved in mesenchymal

tissue formation

6
6
Q66HD0
PREDICTED: TUMOR REJECTION ANTIGEN
74208
Cytoplasm
Molecular chaperone,
Widely distributed
Highly homologous to

GP96

protein folding, stress

endoplasmin precursor

response

6
8
GeneID: 290559
PREDICTED: SIMILAR TO STABILIN-1
288663
Membrane
Extracellular matrix
High levels found
Acts as a scavenger

structural protein,
in human spleen,
receptor for acetylated

extracellular matrix protein-
lymph node, liver
low density lipoprotein.

mediated signaling
and placenta
Binds to both Gram-

positive and Gram-

negative bacteria and

may play a role in

defense against bacterial

infection. When inhibited

in endothelial tube

formation assays, there is

a marked decrease in

cell-cell interactions,

suggesting a role in

angiogenesis

6
7
Q6MG73
COMPLEMENT COMPONENT 2
83699
Secreted
Serine protease,
Plasma
Belongs to the peptidase

complement-mediated

S1 family

immunity

6
9
Q6IRS6
FETUB PROTEIN
43169
Secreted
Cysteine protease inhibitor
Liver
Inhibits insulin receptor

tyrosine kinase activity.

5
6
P62963
PROFILIN-1
14826
Intracellular
Cell structure
Ubiquitous
At high concentrations,

profilin prevents the

polymerization of actin,

whereas it enhances it at

low concentrations

5
5
Q5U300
HYPOTHETICAL PROTEIN LOC314432, Similar
117788
Intracellular
Proteolysis
Ubiquitous
Ubiquitin activating

to ubiquitin-protein ligase (EC 6.3.2.19) E1

enzyme activity

5
12
Q68FT8
SERINE PEPTIDASE INHIBITOR, CLADE F,
54893
Secreted
Serine Protease Inhibitor
Plasma
Belongs to the serpin

MEMBER 2

family

5
8
Q9JI03
COLLAGEN ALPHA-1(V) CHAIN PRECURSOR
184610
Secreted,
Extracellular matrix,
Ubiquitous
Component of connective

extracellular
Structural protein

tissue

space,

extracellular

matrix

5
5
P08649
COMPLEMENT C4 PRECURSOR
192163
Secreted
Complement-mediated
Plasma
Inflammatory response

immunity

5
7
GeneID: 307351
HYPOTHETICAL PROTEIN RGD1305887-
50059
Intracellular
Cell structure, cell mobility,
Ubiquitous
Tubulin is the major

TUBULIN BETA CHAIN

chromosome segregation,

constituent of

intracellular protein traffic

microtubules

5
6
Q6IUU3
QUIESCIN Q6
82412
Isoform 1:
Oxidase
Widely
May contribute to

Membrane;

distributed,
disulfide bond formation

Isoform 2:

expressed in
in a variety of secreted

Secreted

heart, placenta,
proteins

protein

lung, liver,

skeletal muscle,

pancreas and

very weakly in

brain and kidney

5
6
GeneID: 296371
PREDICTED: SIMILAR TO PHOSPHOLIPID
65430
Secreted
Lipid and fatty acid
Plasma
Involved in phospholipid

TRANSFER PROTEIN

transport

transfer in the serum.

5
5
Q63041
ALPHA-1-MACROGLOBULIN
167125
Secreted
Serine protease inhibitor
Plasma
Also known as

PREGNANCY ZONE

PROTEIN

5
5
GeneID: 21846
PREDICTED: TYROSINE KINASE RECEPTOR 1
125210
Cell Membrane
Receptor protein-tyrosine
Unknown
Unknown

kinase

5
26
Q08420
EXTRACELLULAR SUPEROXIDE DISMUTASE
26620
Secreted,
Oxidoreductase - Immunity
Widely distributed
Destroys radicals which

[CU - ZN] PRECURSOR

extracellular
and Defense

are normally produced

space,

within the cells and which

extracellular

are toxic to biological

matrix

systems.

5
11
Q6AYQ9
PEPTIDYLPROLYL ISOMERASE C
23009
Cytoplasm
Isomerase, Protein folding,
Widely
Involved in immunity and

Nuclear transport
distributed, highly
defense

expressed in eye,

vascular tissue,

kidney and brain

5
6
Q62918
PROTEIN KINASE C-BINDING PROTEIN NELL2
91334
Secreted
Cell communication, Cell
Widely distributed
Regulation of growth and

adhesion, Cell structure

neurogenesis

4
7
GeneID: 114489
PREDICTED: DYSTROGLYCAN 1
96706
Membrane
Extracellular matrix protein-
Ubiquitous
Basement membrane

mediated signaling

4
4
O35217
PREDICTED: SIMILAR TO HEPATIC MULTIPLE
54619
Endoplasmic
Nucleotide phosphatase
Widely expressed
Calcium mediated

INOSITOL POLYPHOSPHATE PHOSPHATASE

reticulum

with highest
signaling involved in cell

levels in kidney
proliferation and

and liver
differentiation

4
4
Q91XN5
PROMININ-1S1 SPLICE VARIANT
96632
Cell Membrane
Cellular component, integral
Hematopoeitc
May be involved in

to membrane
stem cells, retina,
membrane traffic. Has

placenta, lung
been localized to

brain
extracellular membrane

bound particles in the

CSF.

4
5
O88754
EPSILON 3 GLOBIN
16540
Secreted
Oxygen Transport, Transfer -
Blood
Involved in oxygen

Carrier Protein

transport from the lung to

the various peripheral

tissues

4
9
P14841
CYSTATIN C PRECURSOR
15437
Secreted,
Cysteine protease inhibitor
Widely distributed
Regulation of

extracellular

with high
programmed cell death

space,

expression in

extracellular

brain and muscle

matrix

4
4
GeneID: 295027
PREDICTED: SIMILAR TO PROTOCADHERIN
123552
Cell Membrane
Cell adhesion
Expressed in
May be involved in the

18 PRECURSOR

brain
establishment and

maintenance of specific

neuronal connections in

the brain

4
7
Q9ERB4
SPLICE ISOFORM V0 OF VERSICAN CORE
300008
Secreted,
Extracellular matrix protein-
Widely distributed
May be involved in axon

PROTEIN PRECURSOR (FRAGMENT)

extracellular
mediated signaling, cell

regeneration and

space,
adhesion, cell motility

physiological response to

extracellular

wounding

matrix

4
5
GeneID: 116782
PROTOCADHERIN GAMMA SUBFAMILY C, 3
101038
Cell Membrane
Cell adhesion
Widely
May play a role during

distributed, highly
spermatogenesis

expressed in

pineal gland

4
4
O54861
SORTILIN PRECURSOR
91169
Membranes;
Functions as a sorting
Highly expressed
Also known as

localized
receptor, endocytosis,
in fat, brain, and
Neurotensin receptor 3

endosomes,
general intracellular vesicle
lung

golgi,
transport

lysosomes and

nucleus

4
4
O88600
ISCHEMIA RESPONSIVE 94 KDA PROTEIN
94057
Intracellular,
Heat shock, protein folding,
Widely distributed
Heat shock 70 kDa

Cytoplasm
stress response

protein 4

4
4
Q63002
MANNOSE 6-PHOSPHATE/INSULIN-LIKE
273608
Cell Membrane
Insulin/IGF signaling
Widely distributed
Soluble receptor found in

GROWTH FACTOR II RECEPTOR

pathway

serum, amniotic fluid and

urine.

4
5
Q91YB6
COMPLEMENT INHIBITORY FACTOR H
140344
Secreted
Complement-mediated
Plasma
Regulation of

immunity

complement activation

4
6
Q62701
TENASCIN (FRAGMENT)
67815
Secreted,
Cell adhesion, extracellular
Widely
Negative regulation of

extracellular
matrix glycoprotein-
distrubuted
cell adhesion. Ligand for

space,
mediated signaling

integrin receptors.

extracellular

matrix

4
4
GeneID: 311416
PREDICTED: SIMILAR TO VINCULIN
116615
Intracellular
Cell adhesion, cell structure
Unknown
Non-motor actin binding

protein

3
3
P63102
14-3-3 PROTEIN ZETA/DELTA
27771
Cytoplasm
Chaperone, signal
Highly expressed
Adapter protein

transduction
in brain
implicated in the

regulation of a large

spectrum of both general

and specialized signaling

pathway

3
4
P07335
CREATINE KINASE B-TYPE
42712
Cytoplasm
Kinase, energy modulation
Brain specific
Phosphocreatine

isoform
metabolism that may be

necessary for brain

development

3
3
P35704
PEROXIREDOXIN-2
21652
Cytoplasm
Oxidoreductase,
Widely
Antiadation and free

Peroxidase
distributed, highly
radical removal

expressed in

bone marrow,

heart, brain,

kidney and

muscle

3
3
GeneID: 366031
PREDICTED: SIMILAR TO CRB2 PROTEIN
138781
Membrane
Membrane-bound signaling
Unknown
Immunity and defense,

molecule?

mRNA transcription

regulation

3
4
P60711
ACTIN, CYTOPLASMIC 1
41737
Cytoplasm
Cell structure, cell mobility,
Ubiquitous
Actins are highly

intracellular protein traffic

conserved proteins that

are involved in various

types of cell motility and

are ubiquitously

expressed in all

eukaryotic cells.

3
4
P35952
LOW-DENSITY LIPOPROTEIN RECEPTOR
96622
Cell Membrane
Lipid and fatty acid
Plasma
Binds LDL, the major

PRECURSOR

transport and metabolism

cholesterol-carrying

lipoprotein of plasma,

and transports it into cells

by endocytosis

3
3
P12785
FATTY ACID SYNTHASE
272650
Intracellular-
Lipid and fatty acid
Ubiquitously
Invovled in catalyzing the

cytoplasm
biosynthesis
expressed
formation of long chain

fatty acids.

3
4
P10111
PEPTIDYL-PROLYL CIS-TRANS ISOMERASE A
17743
Cytoplasm
Isomerase, Protein folding,
Widely
PPlases accelerate the

Nuclear transport
distributed, highly
folding of proteins

expressed in

nerve ganglia

3
4
P07151
BETA-2-MICROGLOBULIN PRECURSOR
13720
Cell Membrane
Immune response, Major
Expressed on
Beta chain of major

histocompatibility complex
nucleated cells
histocompatibility

antigen

complex class I

molecules

3
6
Q62632
FOLLISTATIN-RELATED PROTEIN 1
34622
Secreted
Select regulatory molecule -
Widely
Modulates action of some

PRECURSOR

Homeostatis
distributed, highly
growth factors on cell

expressed in
proliferation and

vascular tissue
differentiation

and nerve ganglia

3
3
Q5M822
SERINE (OR CYSTEINE) PROTEINASE
44671
Secreted
Serine Protease Inhibitor
Plasma
Belongs to the serpin

INHIBITOR, CLADE A (ALPHA-1

family

ANTIPROTEINASE, ANTITRYPSIN), MEMBER 6

3
6
Q63555
SP120 - Heterogeneous nuclear
87748
Intracellular
Transporter activity - RNA
Widely distributed
Nuclear scaffold protein

ribonucleoprotein U

binding

3
6
Q6PED0
RIBOSOMAL PROTEIN S27A
17951
Intracellular
Protein biosynthesis
Ubiquitous
Ribosomal structural

protein

3
4
Q62656
SPLICE ISOFORM 2 OF RECEPTOR-TYPE
164596
Cell Membrane
Cell surface receptor
CNS
May be involved in the

TYROSINE-PROTEIN PHOSPHATASE ZETA

mediated signal

regulation of specific

PRECURSOR

transduction

developmental processes

in the CNS.

3
3
P31000
VIMENTIN
53602
Intracellular
Cell structure
Widely
Found in various non-

distributed, highly
epithelial cells, especially

expressed in
mesenchymal cells

nerve ganglia

3
3
P97536
CULLIN-ASSOCIATED NEDD8-DISSOCIATED
136362
Nucleus
Transcriptional Enhancer
Detected in heart,
Also negative regulator of

PROTEIN 1

brain, spleen,
ubiquitin ligase complex

liver, skeletal

muscle, kidney

and testis

3
3
O70535
LEUKEMIA INHIBITORY FACTOR RECEPTOR
122394
Isoform 1: Cell
Cytokine receptor - Signal
Secreted form
The soluble form inhibits

PRECURSOR

membrane;
transducing molecule
found in plasma.
the biological activity of

Isoform 2:

Membrane form
LIF by blocking its

Secreted

highly expressed
binding to receptors on

in placenta, liver,
target cells

kidney, heart,

lung and brain.

3
3
P11442
CLATHRIN HEAVY CHAIN
191599
Intracellular -
Ligand-mediated signaling,
Ubiquitously
Involved in the formation

vesicle coat
Receptor mediated
expressed
of clathrin coated

endocytosis

vesicles during vesicle

endocytosis

2
3
P01835
IG KAPPA CHAIN C REGION, B ALLELE
11601
Secreted
Immunoglobulin; B-cell and
Lymphoreticular
Immunoglobulin family

antibody-mediated immunity
tissue

2
2
Q5RKI1
EUKARYOTIC TRANSLATION INITIATION
46489
Intracellular
Protein biosynthesis,
Ubiquitous
Required for mRNA

FACTOR 4A2

Translation Initiation factor

binding to ribosome

2
3
P04276
VITAMIN D-BINDING PROTEIN PRECURSOR
53544
Secreted
Transport
Found in plasma,
Binds and carries vitamin

ascitic fluid,
D and also prevents actin

cerebrospinal
polymerization

fluid, and urine

and on the

surface of many

cell types.

2
3
GeneID: 500303
PREDICTED: SIMILAR TO ALPHA ENOLASE
46489
Cytoplasm, Cell
Glycolysis and plasminogen
Expressed in
Multifunctional enzyme

membrane
activation
embryo and in
that plays a part in

most adult
various processes such

tissues, striated
as growth control,

muscle, neurons
hypoxia tolerance and

allergic responses. May

also function in the

fibrinolytic system due to

its ability to serve as a

receptor and activator of

plasminogen on the cell

surface of several cell

types

2
3
Q9R0T4
EPITHELIAL-CADHERIN PRECURSOR
98715
Cell Membrane,
Cell adhesion
Widely
May play a role in axonal

Cell junction

distributed, highly
growth and synapse

expressed in the
formation

colon

2
2
Q6P502
PREDICTED: similar to T-complex protein 1
59745
Cytoplasm
Chaperone
Widely
Belongs to the TCP-1

subunit theta

distributed, highly
chaperonin family

expressed in

brain

2
2
Q66HD3
NUCLEAR AUTOANTIGENIC SPERM PROTEIN
84200
Intracellular
Transport
Widely expressed
Required for DNA

replication, normal cell

cycle progression and

cell proliferation

2
2
GeneID: 363155
PREDICTED: SIMILAR TO PROGRAMMED
75806
Cytoplasm
Transmembrane receptor
Expressed in
DNA damage response,

CELL DEATH 6 INTERACTING PROTEIN

regulatory/adaptor protein
astrocytes and
signal transduction

glioma cells
resulting in induction of

apoptosis

2
2
P34058
HEAT SHOCK PROTEIN HSP 90-BETA
83185
Cytoplasm
Chaperone, protein folding,
Ubiquitous
Belongs to the heat

stress response

shock protein 90 family

2
6
Q71DI1
DERMCIDIN
11284
Secreted
Neuronal survival,
Brain, skin
A maternal blood-borne

phosphatase activity

factor promotes survival

of the developing

thalamus

2
8
Q64240
AMBP PROTEIN PRECURSOR
38851
Secreted
Serine protease inhibitor
Plasma, urine,
It appears not only as a

and cerebrospinal
free monomer but also in

fluid
complexes with IgA and

albumin

2
2
Q8VID2
PREDICTED: AMINOPEPTIDASE PUROMYCIN
103344
Cytoplasm
Metalloprotease -
Widely
Also known as

SENSITIVE

Aminopeptidase activity
distributed, highly
METALLOPROTEASE

detected in
MP100

hippocampus

2
2
GeneID: 293860
PREDICTED: SIMILAR TO FILAMIN A
290169
Intracellular
Cell structure, Cell motility
Ubiquitous
Non-motor actin binding

protein

2
2
Q9JLT0
MYOSIN-10
228965
Intracellular
Cell structure, cell motility
Brain
Actin binding motor

protein

2
4
P15865
HISTONE H1.2
21856
Nucleus
Chromatin packaging and
Ubiquitous
Necessary for

remodeling

condensation of nuclear

DNA

2
2
Q8K586
GTP-BINDING NUCLEAR PROTEIN RAN,
24451
Nucleus
Nucleocytoplasmic
Testis
Required for the import of

TESTIS-SPECIFIC ISOFORM

transport

protein into the nucleus

and also for RNA export:

2
2
P62260
14-3-3 PROTEIN EPSILON
29174
Cytoplasm
Chaperone, signal
Present at high
Adapter protein

transduction
levels in the
implicated in the

pineal gland early
regulation of a large

in development
spectrum of both general

and specialized signaling

pathway

TABLE 4

Protein list from mass spectrometry of all individual samples of embryonic rat CSF collected from E12.5

lateral ventricle (LV), E14.5 lateral ventricle (LV), 4th ventricle (4thV), and E17.5 lateral ventricle (LV).

# of

# of

# of

# of

unique

unique

unique
Total

unique

peptides

peptides

peptides
number

peptides
Total #

from
Total #

from
Total #

from
of
Protein matches E14
from
of

protein
peptides
Protein matches E12.5
protein
peptides
Protein matches E14 LV
protein
peptides
4thV
protein
peptides
Protein matches E17.5 LV

121
587

Rattus norvegicus (Rat)
107
437

Rattus norvegicus (Rat)
120
617

Rattus norvegicus (Rat)
111
414

Rattus norvegicus (Rat)

AA1064-apolipoprotein B.

AA1064-apolipoprotein B.

AA1064-apolipoprotein B.

AA1064-apolipoprotein B.

[MASS = 536024

[MASS = 536024

[MASS = 536024

[MASS = 536024

64
367

Rattus norvegicus (Rat)
62
293

Rattus norvegicus (Rat)
66
382

Rattus norvegicus (Rat)
58
226

Rattus norvegicus (Rat)

Apolipoprotein B -

Apolipoprotein B -

Apolipoprotein B -

Apolipoprotein B - fragment.

fragment. [MASS = 165356

fragment. [MASS = 165356

fragment. [MASS = 165356

[MASS = 165356

34
96

Rattus norvegicus (Rat)
34
73

Rattus norvegicus (Rat)
37
135

Rattus norvegicus (Rat)
45
61

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

SPLICE ISOFORM 1 OF

SPLICE ISOFORM 1 OF

DYNEIN HEAVY CHAIN,

FIBRONECTIN

FIBRONECTIN

FIBRONECTIN

CYTOSOLIC. [MASS = 532252

PRECURSOR.

PRECURSOR.

PRECURSOR.

[MASS = 272511

[MASS = 272511

[MASS = 272511

29
104

Rattus norvegicus (Rat)
32
90

Rattus norvegicus (Rat)
34
365

Rattus norvegicus (Rat)
40
55

Rattus norvegicus (Rat)

APOLIPOPROTEIN A-IV

APOLIPOPROTEIN A-IV

SPLICE ISOFORM 1 OF

PREDICTED: SIMILAR TO

PRECURSOR.

PRECURSOR.

ALPHA-FETOPROTEIN

FILAMIN A. [MASS = 290169

[MASS = 44456

[MASS = 44456

PRECURSOR.

[MASS = 68386

28
32

Rattus norvegicus (Rat)
29
290

Rattus norvegicus (Rat)
31
120

Rattus norvegicus (Rat)
39
44

Rattus norvegicus (Rat) FATTY

PREDICTED: SIMILAR

SPLICE ISOFORM 1 OF

APOLIPOPROTEIN A-IV

ACID SYNTHASE.

TO FILAMIN A.

ALPHA-FETOPROTEIN

PRECURSOR.

[MASS = 272650

[MASS = 290169

PRECURSOR.

[MASS = 44456

[MASS = 68386

27
227

Rattus norvegicus (Rat)
24
54

Rattus norvegicus (Rat)
28
53

Rattus norvegicus (Rat)
39
79

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

ALPHA-2-

ALPHA-2-

SPLICE ISOFORM 1 OF

ALPHA-FETOPROTEIN

MACROGLOBULIN

MACROGLOBULIN

FIBRONECTIN PRECURSOR.

PRECURSOR.

PRECURSOR.

PRECURSOR.

[MASS = 272511

[MASS = 68386

[MASS = 163701

[MASS = 163701

26
62

Rattus norvegicus (Rat)
24
30

Rattus norvegicus (Rat)
21
26

Rattus norvegicus (Rat)
37
99

Rattus norvegicus (Rat)

ALPHA-1-INHIBITOR 3

GPI-ANCHORED

GPI-ANCHORED

ALPHA-2-MACROGLOBULIN

PRECURSOR.

CERULOPLASMIN.

CERULOPLASMIN.

PRECURSOR. [MASS = 163701

[MASS = 163773

[MASS = 123749

[MASS = 123749

26
32

Rattus norvegicus (Rat)
22
38

Rattus norvegicus (Rat)
21
35

Rattus norvegicus (Rat)
33
418

Rattus norvegicus (Rat)

FATTY ACID SYNTHASE.

ALPHA-1-INHIBITOR 3

ALPHA-1-INHIBITOR 3

SPLICE ISOFORM 1 OF

[MASS = 272650

PRECURSOR.

PRECURSOR.

ALPHA-FETOPROTEIN

[MASS = 163773

[MASS = 163773

PRECURSOR. [MASS = 68386

22
33

Rattus norvegicus (Rat)
21
22

Rattus norvegicus (Rat)
20
96

Rattus norvegicus (Rat)
28
30

Rattus norvegicus (Rat)

GPI-ANCHORED

FATTY ACID SYNTHASE.

BA1-667 - Transferrin.

MYOSIN-10. [MASS = 228965

CERULOPLASMIN.

[MASS = 272650

[MASS = 107448

[MASS = 123749

20
123

Rattus norvegicus (Rat)
21
96

Rattus norvegicus (Rat)
19
30

Rattus norvegicus (Rat)
26
78

Rattus norvegicus (Rat)

SERUM ALBUMIN

BA1-667 - Transferrin.

CONTACTIN-2

APOLIPOPROTEIN A-IV

PRECURSOR.

[MASS = 107448

PRECURSOR.

PRECURSOR. [MASS = 44456

[MASS = 68719

[MASS = 113043

19
20

Rattus norvegicus (Rat)
19
60

Rattus norvegicus (Rat)
18
79

Rattus norvegicus (Rat)
25
33

Rattus norvegicus (Rat)

MYOSIN-10.

APOLIPOPROTEIN A-I

APOLIPOPROTEIN A-I

CONTACTIN-1 PRECURSOR.

[MASS = 228965

PRECURSOR.

PRECURSOR.

[MASS = 113495

[MASS = 30088

[MASS = 30088

18
38

Rattus norvegicus (Rat)
19
112

Rattus norvegicus (Rat)
18
97

Rattus norvegicus (Rat)
23
160

Rattus norvegicus (Rat)

HEAT SHOCK PROTEIN

SERUM ALBUMIN

SERUM ALBUMIN

SERUM ALBUMIN

86. [MASS = 84815

PRECURSOR.

PRECURSOR.

PRECURSOR. [MASS = 68719

[MASS = 68719

[MASS = 68719

18
27

Rattus norvegicus (Rat)
17
19

Rattus norvegicus (Rat)
18
33

Rattus norvegicus (Rat)
23
29

Rattus norvegicus (Rat)

ELONGATION FACTOR

PREDICTED: NIDOGEN.

PREDICTED: NIDOGEN.

ALPHA-1-MACROGLOBULIN.

2. [MASS = 95153

[MASS = 138365

[MASS = 138365

[MASS = 167125

18
43

Rattus norvegicus (Rat)
15
38

Rattus norvegicus (Rat)
15
34

Rattus norvegicus (Rat)
20
21

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

ALPHA-1-

ALPHA-1-

MYOSIN-9. [MASS = 226207

TO PYRUVATE KINASE

ANTIPROTEINASE

ANTIPROTEINASE

(EC 2.7.1.40) ISOZYME

PRECURSOR.

PRECURSOR.

M2 - RAT. [MASS = 57731

[MASS = 46136

[MASS = 46136

17
32

Rattus norvegicus (Rat)
15
20

Rattus norvegicus (Rat)
15
17

Rattus norvegicus (Rat)
20
49

Rattus norvegicus (Rat)

ALPHA-1-

CONTACTIN-2

DA1-24-Complement

PREDICTED: SIMILAR TO

ANTIPROTEINASE

PRECURSOR.

Factor B. [MASS = 124379

INTER-ALPHA-INHIBITOR H2

PRECURSOR.

[MASS = 113043

CHAIN. [MASS = 105715

[MASS = 46136

17
25

Rattus norvegicus (Rat)
14
18

Rattus norvegicus (Rat)
14
23

Rattus norvegicus (Rat)
19
47

Rattus norvegicus (Rat)

COMPLEMENT C3

HEAT SHOCK PROTEIN

PREDICTED: SIMILAR

ALPHA-1-ANTIPROTEINASE

PRECURSOR.

86. [MASS = 84815

TO CADHERIN-5.

PRECURSOR. [MASS = 46136

[MASS = 186460

[MASS = 135230

17
63

Rattus norvegicus (Rat)
14
29

Rattus norvegicus (Rat)
13
20

Rattus norvegicus (Rat)
19
24

Rattus norvegicus (Rat)

BA1-667 - Transferrin.

PREDICTED: SIMILAR

ALPHA-2 ANTIPLASMIN.

ELONGATION FACTOR 2.

[MASS = 107448

TO PYRUVATE KINASE

[MASS = 46465

[MASS = 95153

(EC 2.7.1.40) ISOZYME

M2 - RAT. [MASS = 57731

16
56

Rattus norvegicus (Rat)
12
17

Rattus norvegicus (Rat)
13
18

Rattus norvegicus (Rat)
19
19

Rattus norvegicus (Rat)

APOLIPOPROTEIN A-I

ALPHA-2 ANTIPLASMIN.

SPLICE ISOFORM 1 OF

PREDICTED: SIMILAR TO

PRECURSOR.

[MASS = 46465

AGRIN PRECURSOR.

CAD PROTEIN.

[MASS = 30088

[MASS = 208646

[MASS = 250725

16
28

Rattus norvegicus (Rat)
12
24

Rattus norvegicus (Rat)
13
25

Rattus norvegicus (Rat)
18
24

Rattus norvegicus (Rat)

ALPHA-2-

NEURAL-CADHERIN

PREDICTED: SIMILAR

HYPOTHETICAL PROTEIN

MACROGLOBULIN

PRECURSOR.

TO HEPARAN SULFATE

LOC314432-Similar to

PRECURSOR.

[MASS = 99686

PROTEOGLYCAN 2.

ubiquitin-protein ligase (EC

[MASS = 163701

[MASS = 377284

6.3.2.19) E1. [MASS = 117788

16
17

Rattus norvegicus (Rat)
12
22

Rattus norvegicus (Rat)
13
19

Rattus norvegicus (Rat)
18
102

Rattus norvegicus (Rat) BA1-

CLATHRIN HEAVY

PREDICTED: SIMILAR

INTER-ALPHA-

667 - Transferrin.

CHAIN. [MASS = 191599

TO CADHERIN-5.

INHIBITOR H4 HEAVY

[MASS = 107448

[MASS = 135230

CHAIN. [MASS = 103755

14
14

Rattus norvegicus (Rat)
12
14

Rattus norvegicus (Rat)
12
14

Rattus norvegicus (Rat)
17
20

Rattus norvegicus (Rat) DA1-

DYNEIN HEAVY CHAIN,

PREDICTED: SIMILAR

GELSOLIN.

24-Complement Factor B.

CYTOSOLIC.

TO LAMININ B1.

[MASS = 86286

[MASS = 124379

[MASS = 532252

[MASS = 228429

13
23

Rattus norvegicus (Rat)
11
12

Rattus norvegicus (Rat)
12
15

Rattus norvegicus (Rat)
17
34

Rattus norvegicus (Rat)

RAT ALPHA(1)-

GELSOLIN.

SERINE/CYSTEINE

PREDICTED: MICROTUBULE-

INHIBITOR 3, VARIANT I

[MASS = 86286

PROTEINASE

ASSOCIATED PROTEIN 1B.

PRECURSOR.

INHIBITOR, CLADE C,

[MASS = 269643

[MASS = 165326

MEMBER 1.

[MASS = 52234

13
16

Rattus norvegicus (Rat)
11
13

Rattus norvegicus (Rat)
12
22

Rattus norvegicus (Rat)
16
17

Rattus norvegicus (Rat)

PREDICTED: NIDOGEN.

ELONGATION FACTOR

COMPLEMENT C3

CONTACTIN-2 PRECURSOR.

[MASS = 138365

2. [MASS = 95153

PRECURSOR.

[MASS = 113043

[MASS = 186460

13
13

Rattus norvegicus (Rat)
11
18

Rattus norvegicus (Rat)
12
24

Rattus norvegicus (Rat)
16
23

Rattus norvegicus (Rat) HEAT

PREDICTED: SIMILAR

COMPLEMENT C3

APOLIPOPROTEIN E

SHOCK PROTEIN 86.

TO CAD PROTEIN.

PRECURSOR.

PRECURSOR.

[MASS = 84815

[MASS = 250725

[MASS = 186460

[MASS = 35753

13
15

Rattus norvegicus (Rat)
11
13

Rattus norvegicus (Rat)
11
14

Rattus norvegicus (Rat)
16
17

Rattus norvegicus (Rat)

VIMENTIN. [MASS = 53602

TRANSITIONAL

LAR RECEPTOR-

CLATHRIN HEAVY CHAIN.

ENDOPLASMIC

LINKED TYROSINE

[MASS = 191599

RETICULUM ATPASE.

PHOSPHATASE.

[MASS = 89534

[MASS = 181130

13
14

Rattus norvegicus (Rat)
11
11

Rattus norvegicus (Rat)
11
12

Rattus norvegicus (Rat)
15
25

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

NUCLEAR

DELETED IN

COMPLEMENT C3

TO GCN1 GENERAL

AUTOANTIGENIC

COLORECTAL CANCER.

PRECURSOR. [MASS = 186460

CONTROL OF AMINO-

SPERM PROTEIN.

[MASS = 158142

ACID SYNTHESIS 1-

[MASS = 84200

LIKE 1. [MASS = 302942

12
12

Rattus norvegicus (Rat)
11
18

Rattus norvegicus (Rat)
11
12

Rattus norvegicus (Rat)
15
15

Rattus norvegicus (Rat)

HYPOTHETICAL

CORTICOSTEROID-

SERINE PEPTIDASE

PREDICTED: SIMILAR TO

PROTEIN LOC314432-

BINDING GLOBULIN

INHIBITOR, CLADE G,

GCN1 GENERAL CONTROL

Similar to ubiquitin-protein

PRECURSOR.

MEMBER 1.

OF AMINO-ACID SYNTHESIS

ligase (EC 6.3.2.19) E1.

[MASS = 44672

[MASS = 55611

1-LIKE 1. [MASS = 302942

[MASS = 117788

12
17

Rattus norvegicus (Rat)
11
22

Rattus norvegicus (Rat)
11
17

Rattus norvegicus (Rat)
14
32

Rattus norvegicus (Rat)

ATP-CITRATE

APOLIPOPROTEIN E

CORTICOSTEROID-

APOLIPOPROTEIN A-I

SYNTHASE.

PRECURSOR.

BINDING GLOBULIN

PRECURSOR. [MASS = 30088

[MASS = 120781

[MASS = 35753

PRECURSOR.

[MASS = 44672

12
15

Rattus norvegicus (Rat)
10
47

Rattus norvegicus (Rat)
11
12

Rattus norvegicus (Rat)
14
46

Rattus norvegicus (Rat)

TRANSITIONAL

TRANSTHYRETIN

PREDICTED: SIMILAR

CORTICOSTEROID-BINDING

ENDOPLASMIC

PRECURSOR.

TO LAMININ B1.

GLOBULIN PRECURSOR.

RETICULUM ATPASE.

[MASS = 15720

[MASS = 228429

[MASS = 44672

[MASS = 89534

11
14

Rattus norvegicus (Rat)
10
17

Rattus norvegicus (Rat)
11
11

Rattus norvegicus (Rat)
14
15

Rattus norvegicus (Rat)

ALPHA-2 ANTIPLASMIN.

RAT ALPHA(1)-

ELONGATION FACTOR

CULLIN-ASSOCIATED

[MASS = 46465

INHIBITOR 3, VARIANT I

2. [MASS = 95153

NEDD8-DISSOCIATED

PRECURSOR.

PROTEIN 1. [MASS = 136362

[MASS = 165326

11
12

Rattus norvegicus (Rat)
10
13

Rattus norvegicus (Rat)
11
14

Rattus norvegicus (Rat)
14
42

Rattus norvegicus (Rat)

IMPORTIN BETA-1

CONTRAPSIN-LIKE

SPLICE ISOFORM 1 OF

TUBULIN ALPHA-1 CHAIN.

SUBUNIT. [MASS = 97184

PROTEASE INHIBITOR 6

FIBRINOGEN ALPHA

[MASS = 50136

PRECURSOR.

CHAIN PRECURSOR.

[MASS = 46652

[MASS = 86686

11
14

Rattus norvegicus (Rat)
10
12

Rattus norvegicus (Rat)
11
16

Rattus norvegicus (Rat)
14
14

Rattus norvegicus (Rat) GPI-

CULLIN-ASSOCIATED

PREDICTED: SIMILAR

PREDICTED: SIMILAR

ANCHORED

NEDD8-DISSOCIATED

TO KINESIN FAMILY

TO ALPHA 3 TYPE VI

CERULOPLASMIN.

PROTEIN 1.

MEMBER 23.

COLLAGEN ISOFORM 1

[MASS = 123749

[MASS = 136362

[MASS = 108791

PRECURSOR.

[MASS = 369017

11
40

Rattus norvegicus (Rat)
10
16

Rattus norvegicus (Rat)
10
21

Rattus norvegicus (Rat)
14
28

Rattus norvegicus (Rat)

TUBULIN ALPHA-1

COLLAGEN ALPHA-1(I)

NEURAL-CADHERIN

ECTONUCLEOTIDE

CHAIN. [MASS = 50136

CHAIN PRECURSOR.

PRECURSOR.

PYROPHOSPHATASE/

[MASS = 137886

[MASS = 99686

PHOSPHODIESTERASE 2.

[MASS = 101310

11
18

Rattus norvegicus (Rat)
10
10

Rattus norvegicus (Rat)
10
14

Rattus norvegicus (Rat)
13
18

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

CLATHRIN HEAVY

PREDICTED: LAMININ,

GELSOLIN. [MASS = 86286

TO FIBULIN-1

CHAIN. [MASS = 191599

GAMMA 1.

PRECURSOR.

[MASS = 177387

[MASS = 78072

11
18

Rattus norvegicus (Rat)
9
20

Rattus norvegicus (Rat)
10
22

Rattus norvegicus (Rat)
13
15

Rattus norvegicus (Rat)

APOLIPOPROTEIN E

TUBULIN ALPHA-1

PREDICTED: SIMILAR

VIMENTIN. [MASS = 53602

PRECURSOR.

CHAIN. [MASS = 50136

TO FIBULIN-1

[MASS = 35753

PRECURSOR.

[MASS = 78072

11
11

Rattus norvegicus (Rat)
9
9

Rattus norvegicus (Rat)
10
11

Rattus norvegicus (Rat)
12
21

Rattus norvegicus (Rat)

Glutamyl-prolyl-tRNA

LAR RECEPTOR-

ECTONUCLEOTIDE

TRANSITIONAL

synthetase.

LINKED TYROSINE

PYROPHOSPHATASE/

ENDOPLASMIC RETICULUM

[MASS = 170088

PHOSPHATASE.

PHOSPHODIESTERASE

ATPASE. [MASS = 89534

[MASS = 181130

2. [MASS = 101310

11
11

Rattus norvegicus (Rat)
9
11

Rattus norvegicus (Rat)
10
21

Rattus norvegicus (Rat)
12
12

Rattus norvegicus (Rat)

MYOSIN-9.

IMPORTIN BETA-1

COLLAGEN ALPHA-1(I)

COMPLEMENT COMPONENT

[MASS = 226207

SUBUNIT. [MASS = 97184

CHAIN PRECURSOR.

2. [MASS = 83699

[MASS = 137886

11
15

Rattus norvegicus (Rat)
9
9

Rattus norvegicus (Rat)
10
21

Rattus norvegicus (Rat)
12
15

Rattus norvegicus (Rat)

INTER-ALPHA-

PREDICTED: VON

LUMICAN PRECURSOR.

PREDICTED: SIMILAR TO

INHIBITOR H4 HEAVY

WILLEBRAND FACTOR.

[MASS = 38279

DESMOPLAKIN ISOFORM II.

CHAIN. [MASS = 103755

[MASS = 308474

[MASS = 264186

10
12

Rattus norvegicus (Rat)
9
15

Rattus norvegicus (Rat)
9
51

Rattus norvegicus (Rat)
12
15

Rattus norvegicus (Rat)

GELSOLIN.

PREDICTED: SIMILAR

TRANSTHYRETIN

PREDICTED: SIMILAR TO

[MASS = 86286

TO HEPARAN SULFATE

PRECURSOR.

ALPHA 3 TYPE VI COLLAGEN

PROTEOGLYCAN 2.

[MASS = 15720

ISOFORM 1 PRECURSOR.

[MASS = 377284

[MASS = 369017

10
10

Rattus norvegicus (Rat)
9
9

Rattus norvegicus (Rat)
9
13

Rattus norvegicus (Rat)
11
11

Rattus norvegicus (Rat)

NUCLEAR

PREDICTED: LAMININ,

CATHEPSIN B

PREDICTED: SIMILAR TO

AUTOANTIGENIC

GAMMA 1.

PRECURSOR.

COATOMER PROTEIN

SPERM PROTEIN.

[MASS = 177387

[MASS = 37470

COMPLEX SUBUNIT ALPHA.

[MASS = 84200

[MASS = 138360

10
16

Rattus norvegicus (Rat)
9
20

Rattus norvegicus (Rat)
9
10

Rattus norvegicus (Rat)
11
19

Rattus norvegicus (Rat)

SP120-Heterogeneous

LUMICAN PRECURSOR.

FIBRINOGEN BETA

CLUSTERIN PRECURSOR.

nuclear ribonucleoprotein

[MASS = 38279

CHAIN PRECURSOR.

[MASS = 51375

U. [MASS = 87748

[MASS = 54303

10
11

Rattus norvegicus (Rat)
9
9

Rattus norvegicus (Rat)
9
13

Rattus norvegicus (Rat)
11
16

Rattus norvegicus (Rat)

CONTACTIN-2

Neogenin precursor.

RAT ALPHA(1)-

PREDICTED: SIMILAR TO

PRECURSOR.

[MASS = 156144

INHIBITOR 3, VARIANT I

PYRUVATE KINASE (EC

[MASS = 113043

PRECURSOR.

2.7.1.40) ISOZYME M2 - RAT.

[MASS = 165326

[MASS = 57731

9
10

Rattus norvegicus (Rat)
9
16

Rattus norvegicus (Rat)
9
11

Rattus norvegicus (Rat)
11
15

Rattus norvegicus (Rat)

PHOSPHOGLYCERATE

PREDICTED: SIMILAR

PREDICTED: VON

ALPHA-1-INHIBITOR 3

KINASE 1. [MASS = 44423

TO FIBULIN-1

WILLEBRAND FACTOR.

PRECURSOR. [MASS = 163773

PRECURSOR.

[MASS = 308474

[MASS = 78072

9
10

Rattus norvegicus (Rat)
9
10

Rattus norvegicus (Rat)
9
18

Rattus norvegicus (Rat)
11
11

Rattus norvegicus (Rat)

HEAT SHOCK COGNATE

DA1-24-Complement

LOC367586 PROTEIN-

HEPHAESTIN PRECURSOR.

71 KDA PROTEIN.

Factor B. [MASS = 124379

Immunoglobulin Gamma

[MASS = 129593

[MASS = 70871

heavy Chain.

[MASS = 50949

9
22

Rattus norvegicus (Rat)
9
12

Rattus norvegicus (Rat)
9
11

Rattus norvegicus (Rat)
11
36

Rattus norvegicus (Rat)

TUBULIN BETA-5 CHAIN.

ACTIN, ALPHA

SPLICE ISOFORM LONG

LOC367586 PROTEIN-

[MASS = 49671

SKELETAL MUSCLE.

OF HYALURONAN AND

Immunoglobulin Gamma heavy

[MASS = 42051

PROTEOGLYCAN LINK

Chain. [MASS = 50949

PROTEIN 1

PRECURSOR.

[MASS = 40262

9
12

Rattus norvegicus (Rat)
9
13

Rattus norvegicus (Rat)
8
10

Rattus norvegicus (Rat)
11
19

Rattus norvegicus (Rat) ACTIN,

CONTRAPSIN-LIKE

INTER-ALPHA-

HEAT SHOCK PROTEIN

ALPHA SKELETAL MUSCLE.

PROTEASE INHIBITOR 6

INHIBITOR H4 HEAVY

86. [MASS = 84815

[MASS = 42051

PRECURSOR.

CHAIN. [MASS = 103755

[MASS = 46652

9
19

Rattus norvegicus (Rat)
8
16

Rattus norvegicus (Rat)
8
8

Rattus norvegicus (Rat)
10
20

Rattus norvegicus (Rat)

HYPOTHETICAL

TUBULIN BETA-5 CHAIN.

HEPHAESTIN

NEURAL-CADHERIN

PROTEIN RGD1305887-

[MASS = 49671

PRECURSOR.

PRECURSOR. [MASS = 99686

TUBULIN BETA CHAIN.

[MASS = 129593

[MASS = 50059

9
11

Rattus norvegicus (Rat)
8
14

Rattus norvegicus (Rat)
8
13

Rattus norvegicus (Rat)
10
105

Rattus norvegicus (Rat)

PROMININ-1S1 SPLICE

CONTRAPSIN-LIKE

COLLAGEN ALPHA-1(III)

TRANSTHYRETIN

VARIANT. [MASS = 96632

PROTEASE INHIBITOR 1

CHAIN PRECURSOR.

PRECURSOR. [MASS = 15720

PRECURSOR.

[MASS = 138936

[MASS = 46562

9
9

Rattus norvegicus (Rat)
8
14

Rattus norvegicus (Rat)
8
9

Rattus norvegicus (Rat)
10
11

Rattus norvegicus (Rat)

IKAP. [MASS = 149171

SERINE PEPTIDASE

SPLICE ISOFORM 1 OF

IMPORTIN BETA-1 SUBUNIT.

INHIBITOR, CLADE G,

REELIN PRECURSOR.

[MASS = 97184

MEMBER 1.

[MASS = 387531

[MASS = 55611

9
10

Rattus norvegicus (Rat)
8
9

Rattus norvegicus (Rat)
8
20

Rattus norvegicus (Rat)
10
10

Rattus norvegicus (Rat)

PREDICTED: NEURAL

ISCHEMIA RESPONSIVE

PLASMINOGEN

PREDICTED: SIMILAR TO

PRECURSOR CELL

94 KDA PROTEIN.

PRECURSOR.

CELLULAR APOPTOSIS

EXPRESSED,

[MASS = 94057

[MASS = 90536

SUSCEPTIBILITY PROTEIN.

DEVELOPMENTALLY

[MASS = 110214

DOWN-REGULATED

GENE 4A.

[MASS = 112368

9
18

Rattus norvegicus (Rat)
8
8

Rattus norvegicus (Rat)
8
12

Rattus norvegicus (Rat)
10
11

Rattus norvegicus (Rat)

ACTIN, ALPHA

HYPOTHETICAL

EPSILON 1 GLOBIN.

NUCLEAR AUTOANTIGENIC

SKELETAL MUSCLE.

PROTEIN LOC314432-

[MASS = 16105

SPERM PROTEIN.

[MASS = 42051

Similar to ubiquitin-protein

[MASS = 84200

ligase (EC 6.3.2.19) E1.

[MASS = 117788

9
27

Rattus norvegicus (Rat)
8
10

Rattus norvegicus (Rat)
8
13

Rattus norvegicus (Rat)
10
11

Rattus norvegicus (Rat)

ELONGATION FACTOR

HEPHAESTIN

SPLICE ISOFORM HMW

ISCHEMIA RESPONSIVE 94 KDA

1-ALPHA 1.

PRECURSOR.

OF KININOGEN-1

PROTEIN. [MASS = 94057

[MASS = 50114

[MASS = 129593

PRECURSOR.

[MASS = 70933

9
12

Rattus norvegicus (Rat)
8
15

Rattus norvegicus (Rat)
8
19

Rattus norvegicus (Rat)
10
10

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PLASMINOGEN

SPLICE ISOFORM

PREDICTED: SIMILAR TO

TO HEPARAN SULFATE

PRECURSOR.

GAMMA-B OF

RAS GTPASE-ACTIVATING-

PROTEOGLYCAN 2.

[MASS = 90536

FIBRINOGEN GAMMA

LIKE PROTEIN IQGAP1.

[MASS = 377284

CHAIN PRECURSOR.

[MASS = 196522

[MASS = 50633

9
9

Rattus norvegicus (Rat)
8
8

Rattus norvegicus (Rat)
8
9

Rattus norvegicus (Rat)
10
11

Rattus norvegicus (Rat)

SPLICE ISOFORM LONG

CADHERIN-6

CADHERIN-6

PREDICTED: SIMILAR TO

OF HYALURONAN AND

PRECURSOR.

PRECURSOR.

UBIQUITIN CARBOXYL-

PROTEOGLYCAN LINK

[MASS = 88341

[MASS = 88341

TERMINAL HYDROLASE 5.

PROTEIN 1

[MASS = 95779

PRECURSOR.

[MASS = 40262

8
32

Rattus norvegicus (Rat)
8
10

Rattus norvegicus (Rat)
7
9

Rattus norvegicus (Rat)
10
12

Rattus norvegicus (Rat)

TRANSTHYRETIN

ECTONUCLEOTIDE

TUBULIN BETA-5 CHAIN.

PREDICTED:

PRECURSOR.

PYROPHOSPHATASE/

[MASS = 49671

AMINOPEPTIDASE

[MASS = 15720

PHOSPHODIESTERASE

PUROMYCIN SENSITIVE.

2. [MASS = 101310

[MASS = 103344

8
8

Rattus norvegicus (Rat)
8
10

Rattus norvegicus (Rat)
7
8

Rattus norvegicus (Rat)
10
10

Rattus norvegicus (Rat)

ISCHEMIA RESPONSIVE

PROMININ-1S1 SPLICE

ANGIOTENSINOGEN

COATOMER SUBUNIT BETA.

94 KDA PROTEIN.

VARIANT. [MASS = 96632

PRECURSOR.

[MASS = 107011

[MASS = 94057

[MASS = 51982

8
12

Rattus norvegicus (Rat)
8
9

Rattus norvegicus (Rat)
7
8

Rattus norvegicus (Rat)
10
13

Rattus norvegicus (Rat)

NEURAL-CADHERIN

SPLICE ISOFORM 1 OF

SPARC-LIKE PROTEIN 1

SPLICE ISOFORM 1 OF

PRECURSOR.

AGRIN PRECURSOR.

PRECURSOR.

AGRIN PRECURSOR.

[MASS = 99686

[MASS = 208646

[MASS = 70634

[MASS = 208646

8
15

Rattus norvegicus (Rat)
8
20

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)
10
14

Rattus norvegicus (Rat)

CONTRAPSIN-LIKE

LOC367586 PROTEIN-

Neogenin precursor.

PREDICTED: SIMILAR TO

PROTEASE INHIBITOR 3

Immunoglobulin Gamma

[MASS = 156144

GAMMA-FILAMIN.

PRECURSOR.

heavy Chain.

[MASS = 290986

[MASS = 46277

[MASS = 50949

8
10

Rattus norvegicus (Rat)
8
10

Rattus norvegicus (Rat)
7
16

Rattus norvegicus (Rat)
10
10

Rattus norvegicus (Rat) 284 KDA

LUMICAN PRECURSOR.

VIMENTIN.

CONTRAPSIN-LIKE

PROTEIN.

[MASS = 38279

[MASS = 53602

PROTEASE INHIBITOR 3

[MASS = 284430

PRECURSOR.

[MASS = 46277

8
8

Rattus norvegicus (Rat)
7
12

Rattus norvegicus (Rat)
7
9

Rattus norvegicus (Rat)
9
18

Rattus norvegicus (Rat)

PREDICTED: MINI

CONTRAPSIN-LIKE

ACTIN, ALPHA

TUBULIN BETA-5 CHAIN.

CHROMOSOME

PROTEASE INHIBITOR 3

SKELETAL MUSCLE.

[MASS = 49671

MAINTENANCE

PRECURSOR.

[MASS = 42051

DEFICIENT 6.

[MASS = 46277

[MASS = 92815

8
11

Rattus norvegicus (Rat)
7
9

Rattus norvegicus (Rat)
7
9

Rattus norvegicus (Rat)
9
11

Rattus norvegicus (Rat)

SERINE PEPTIDASE

PREDICTED: NIDOGEN

ELONGATION FACTOR

SERINE PEPTIDASE

INHIBITOR, CLADE G,

2. [MASS = 173960

1-ALPHA 1.

INHIBITOR, CLADE G,

MEMBER 1.

[MASS = 50114

MEMBER 1. [MASS = 55611

[MASS = 55611

8
10

Rattus norvegicus (Rat)
7
8

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)
9
10

Rattus norvegicus (Rat) 170 KDA

PREDICTED: LAMININ,

SPLICE ISOFORM 1 OF

TRANSITIONAL

PROTEIN-Glutamyl-prolyl-

GAMMA 1.

REELIN PRECURSOR.

ENDOPLASMIC

tRNA synthetase.

[MASS = 177387

[MASS = 387531

RETICULUM ATPASE.

[MASS = 170088

[MASS = 89534

8
8

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)
7
11

Rattus norvegicus (Rat)
9
10

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED:

COLLAGEN ALPHA-2(I)

PROTEASOME (PROSOME,

TO RNA HELICASE A.

TRANSFORMING

CHAIN PRECURSOR.

MACROPAIN) 26S SUBUNIT,

[MASS = 150362

GROWTH FACTOR,

[MASS = 129564

NON-ATPASE, 2.

BETA INDUCED, 68 KDA.

[MASS = 100188

[MASS = 74369

8
11

Rattus norvegicus (Rat)
7
10

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)
9
10

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

SERINE/CYSTEINE

ALPHA-MANNOSIDASE

PREDICTED: SIMILAR TO

TO CADHERIN-5.

PROTEINASE

2. [MASS = 131242

RNA HELICASE A.

[MASS = 135230

INHIBITOR, CLADE C,

[MASS = 150362

MEMBER 1.

[MASS = 52234

8
12

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)
7
9

Rattus norvegicus (Rat)
9
9

Rattus norvegicus (Rat)

EPSILON 1 GLOBIN.

IKAP. [MASS = 149171

SPLICE ISOFORM 1 OF

DELETED IN COLORECTAL

[MASS = 16105

ATTRACTIN

CANCER. [MASS = 158142

PRECURSOR.

[MASS = 163296

8
8

Rattus norvegicus (Rat)
7
8

Rattus norvegicus (Rat)
6
9

Rattus norvegicus (Rat)
9
10

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

DELETED IN

TUBULIN ALPHA-1

ALPHA-MANNOSIDASE 2.

TO METHIONINE-TRNA

COLORECTAL CANCER.

CHAIN. [MASS = 50136

[MASS = 131242

SYNTHETASE.

[MASS = 158142

[MASS = 101582

8
8

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)
8
13

Rattus norvegicus (Rat)

EXPORTIN-1.

COMPLEMENT C4

CONTRAPSIN-LIKE

VITAMIN D-BINDING

[MASS = 123335

PRECURSOR.

PROTEASE INHIBITOR 6

PROTEIN PRECURSOR.

[MASS = 192163

PRECURSOR.

[MASS = 53544

[MASS = 46652

8
12

Rattus norvegicus (Rat)
7
13

Rattus norvegicus (Rat)
6
8

Rattus norvegicus (Rat)
8
11

Rattus norvegicus (Rat) IRON-

LOC367586 PROTEIN-

HYPOTHETICAL

SPLICE ISOFORM 1 OF

RESPONSIVE ELEMENT-

Immunoglobulin Gamma

PROTEIN RGD1305887-

NEURONAL CELL

BINDING PROTEIN 1.

heavy Chain.

TUBULIN BETA CHAIN.

ADHESION MOLECULE

[MASS = 98128

[MASS = 50949

[MASS = 50059

PRECURSOR.

[MASS = 133912

8
8

Rattus norvegicus (Rat)
7
12

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)
8
8

Rattus norvegicus (Rat)

PREDICTED:

SP120-Heterogeneous

PREDICTED: SIMILAR

ALPHA-2 ANTIPLASMIN.

AMINOPEPTIDASE

nuclear ribonucleoprotein

TO RIKEN CDNA

[MASS = 46465

PUROMYCIN SENSITIVE.

U. [MASS = 87748

B430218L07 GENE.

[MASS = 103344

[MASS = 143906

8
9

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)
8
13

Rattus norvegicus (Rat) SP120-

PREDICTED: similar to T-

COLLAGEN ALPHA-2(I)

PREDICTED:

Heterogeneous nuclear

complex protein 1 subunit

CHAIN PRECURSOR.

TRANSFORMING

ribonucleoprotein U.

theta. [MASS = 59745

[MASS = 129564

GROWTH FACTOR,

[MASS = 87748

BETA INDUCED, 68 KDA.

[MASS = 74369

8
8

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)
6
8

Rattus norvegicus (Rat)
8
8

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: similar to T-

ALPHA-2-GLOBIN

PREDICTED: MINI

TO FILAMIN B.

complex protein 1 subunit

CHAIN. [MASS = 15285

CHROMOSOME

[MASS = 291469

theta. [MASS = 59745

MAINTENANCE DEFICIENT 6.

[MASS = 92815

8
8

Rattus norvegicus (Rat)
6
8

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)
8
9

Rattus norvegicus (Rat)

STAPHYLOCOCCAL

CATHEPSIN B

ARCADLIN.

PREDICTED: SIMILAR TO

NUCLEASE DOMAIN-

PRECURSOR.

[MASS = 103800

FIBULIN-1 PRECURSOR.

CONTAINING PROTEIN

[MASS = 37470

[MASS = 78072

1. [MASS = 101952

8
9

Rattus norvegicus (Rat)
6
8

Rattus norvegicus (Rat)
6
9

Rattus norvegicus (Rat)
8
8

Rattus norvegicus (Rat)

SPLICE ISOFORM V0 OF

ANGIOTENSINOGEN

TENASCIN

STAPHYLOCOCCAL

VERSICAN CORE

PRECURSOR.

(FRAGMENT).

NUCLEASE DOMAIN-

PROTEIN PRECURSOR

[MASS = 51982

[MASS = 62473

CONTAINING PROTEIN 1.

(FRAGMENT).

[MASS = 101952

[MASS = 300008

8
14

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)
6
12

Rattus norvegicus (Rat)
8
13

Rattus norvegicus (Rat)

FETUB PROTEIN.

RAT T-KININOGEN.

PREDICTED: RETINOL

APOLIPOPROTEIN E

[MASS = 43169

[MASS = 47618

BINDING PROTEIN 4,

PRECURSOR. [MASS = 35753

PLASMA. [MASS = 50139

8
8

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)
6
10

Rattus norvegicus (Rat)
8
8

Rattus norvegicus (Rat)

DA1-24-Complement

TENASCIN

PREDICTED:

PREDICTED: BRAIN

Factor B. [MASS = 124379

(FRAGMENT).

CALSYNTENIN 1.

GLYCOGEN

[MASS = 62473

[MASS = 109351

PHOSPHORYLASE.

[MASS = 96738

7
9

Rattus norvegicus (Rat)
6
8

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)
8
23

Rattus norvegicus (Rat)

RAT T-KININOGEN.

SPLICE ISOFORM 1 OF

SPLICE ISOFORM 1 OF

PLASMINOGEN

[MASS = 47618

ATTRACTIN

ALPHA-1B-

PRECURSOR. [MASS = 90536

PRECURSOR.

GLYCOPROTEIN

[MASS = 163296

PRECURSOR.

[MASS = 56479

7
9

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)
6
8

Rattus norvegicus (Rat)
8
8

Rattus norvegicus (Rat) IKAP,

SERINE/CYSTEINE

PREDICTED: SIMILAR

PREDICTED: NIDOGEN

[MASS = 149171

PROTEINASE

TO RIKEN CDNA

2. [MASS = 173960

INHIBITOR, CLADE C,

B430218L07 GENE.

MEMBER 1.

[MASS = 143906

[MASS = 52234

7
8

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)
8
9

Rattus norvegicus (Rat)

CLIP-ASSOCIATING

PROFILIN-1.

PREDICTED: SIMILAR

MANNOSE 6-

PROTEIN 2.

[MASS = 14826

TO PYRUVATE KINASE

PHOSPHATE/INSULIN-LIKE

[MASS = 140638

(EC 2.7.1.40) ISOZYME

GROWTH FACTOR II

M2 - RAT. [MASS = 57731

RECEPTOR. [MASS = 273608

7
9

Rattus norvegicus (Rat)
6
9

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)
7
11

Rattus norvegicus (Rat)

RIBONUCLEOTIDE

PREDICTED: CADHERIN

RAT T-KININOGEN.

CREATINE KINASE B-TYPE.

REDUCTASE M1.

11. [MASS = 88036

[MASS = 47618

[MASS = 42712

[MASS = 90293

7
7

Rattus norvegicus (Rat)
6
10

Rattus norvegicus (Rat)
6
10

Rattus norvegicus (Rat)
7
13

Rattus norvegicus (Rat)

ECTONUCLEOTIDE

SPLICE ISOFORM

PREDICTED: CADHERIN

HYPOTHETICAL PROTEIN

PYROPHOSPHATASE/

GAMMA-B OF

11. [MASS = 88036

RGD1305887-TUBULIN BETA

PHOSPHODIESTERASE 2.

FIBRINOGEN GAMMA

CHAIN. [MASS = 50059

[MASS = 101310

CHAIN PRECURSOR.

[MASS = 50633

7
8

Rattus norvegicus (Rat)
6
12

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)
7
8

Rattus norvegicus (Rat)

HEPHAESTIN

ELONGATION FACTOR

PREDICTED: TUMOR

PREDICTED: similar to

PRECURSOR.

1-ALPHA 1.

REJECTION ANTIGEN

phosphoribosylformylglycinamidine

[MASS = 129593

[MASS = 50114

GP96. [MASS = 92771

synthase. [MASS = 146178

7
7

Rattus norvegicus (Rat)
6
8

Rattus norvegicus (Rat)
6
8

Rattus norvegicus (Rat)
7
12

Rattus norvegicus (Rat) ATP-

NONO/P54NRB

PREDICTED: SIMILAR

PREDICTED: SIMILAR

CITRATE SYNTHASE.

HOMOLOG.

TO CRB2 PROTEIN.

TO STABILIN-1.

[MASS = 120781

[MASS = 75487

[MASS = 138781

[MASS = 288663

7
14

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)
7
10

Rattus norvegicus (Rat)

PREDICTED:

TRIPEPTIDYL-

PREDICTED: SIMILAR

ANGIOTENSINOGEN

MICROTUBULE-

PEPTIDASE 2.

TO HEMICENTIN 1.

PRECURSOR. [MASS = 51982

ASSOCIATED PROTEIN

[MASS = 138162

[MASS = 639647

1B. [MASS = 269643

7
7

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)
7
10

Rattus norvegicus (Rat)

PREDICTED

PREDICTED: SIMILAR

COMPLEMENT

PREDICTED: SIMILAR TO

NUCLEOLIN-RELATED

TO CAD PROTEIN.

COMPONENT 2.

CADHERIN-5. [MASS = 135230

PROTEIN NRP.

[MASS = 250725

[MASS = 83699

[MASS = 57036

7
8

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)
6
9

Rattus norvegicus (Rat)
7
12

Rattus norvegicus (Rat)

NESTIN. [MASS = 208797

PREDICTED: SIMILAR

FETUB PROTEIN.

PREDICTED: SIMILAR TO

TO RNA HELICASE A.

[MASS = 43169

PHOSPHOLIPID TRANSFER

[MASS = 150362

PROTEIN. [MASS = 65430

7
7

Rattus norvegicus (Rat)
5
8

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

CREATINE KINASE B-

PROFILIN-1.

SEZ6B. [MASS = 105607

AGRIN PRECURSOR.

TYPE. [MASS = 42712

[MASS = 14826

[MASS = 208646

7
8

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
9

Rattus norvegicus (Rat)
7
8

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

PHOSPHOGLYCERATE

CONTRAPSIN-LIKE

DYNACTIN-1. [MASS = 141930

CULLIN-ASSOCIATED

KINASE 1. [MASS = 44423

PROTEASE INHIBITOR 1

NEDD8-DISSOCIATED

PRECURSOR.

PROTEIN 2.

[MASS = 46562

[MASS = 139673

6
10

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
7
8

Rattus norvegicus (Rat) INTER-

CREATINE KINASE B-

HEAT SHOCK COGNATE

PROPROTEIN

ALPHA-INHIBITOR H4 HEAVY

TYPE. [MASS = 42712

71 KDA PROTEIN.

CONVERTASE

CHAIN. [MASS = 103755

[MASS = 70871

SUBTILISIN/KEXIN TYPE

9 PRECURSOR.

[MASS = 74709

6
6

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR

HYPOTHETICAL

SPECTRIN ALPHA CHAIN,

TO SPLICING FACTOR

TO PROGRAMMED

PROTEIN LOC314432-

BRAIN. [MASS = 284713

3B, SUBUNIT 3, 130 KDA.

CELL DEATH 6

Similar to ubiquitin-protein

[MASS = 174174

INTERACTING

ligase (EC 6.3.2.19) E1.

PROTEIN. [MASS = 75806

[MASS = 117788

6
8

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
12

Rattus norvegicus (Rat)
7
10

Rattus norvegicus (Rat)

GLUCOSE PHOSPHATE

FIBRINOGEN BETA

SERINE PEPTIDASE

ELONGATION FACTOR 1-

ISOMERASE.

CHAIN PRECURSOR.

INHIBITOR, CLADE F,

ALPHA 1. [MASS = 50114

[MASS = 62827

[MASS = 54303

MEMBER 2.

[MASS = 54893

6
11

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
8

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)

CONTRAPSIN-LIKE

ATP-CITRATE

COLLAGEN ALPHA-1(V)

PREDICTED: SIMILAR TO

PROTEASE INHIBITOR 1

SYNTHASE.

CHAIN PRECURSOR.

VERY LARGE G PROTEIN-

PRECURSOR.

[MASS = 120781

[MASS = 184610

COUPLED RECEPTOR 1.

[MASS = 46562

[MASS = 413840

6
9

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
7
7

Rattus norvegicus (Rat)

PLASMINOGEN

SPLICE ISOFORM 1 OF

COMPLEMENT C4

PROTEIN KINASE C-BINDING

PRECURSOR.

NEURONAL CELL

PRECURSOR.

PROTEIN NELL2.

[MASS = 90536

ADHESION MOLECULE

[MASS = 192163

[MASS = 91334

PRECURSOR.

[MASS = 133912

6
7

Rattus norvegicus (Rat)
5
11

Rattus norvegicus (Rat)
5
7

Rattus norvegicus (Rat)
7
9

Rattus norvegicus (Rat)

Inter-alpha trypsin

CYSTATIN C

GLUTATHIONE

COMPLEMENT INHIBITORY

inhibitor, heavy chain 3.

PRECURSOR.

PEROXIDASE 3

FACTOR H. [MASS = 140344

[MASS = 98968

[MASS = 15437

PRECURSOR.

[MASS = 25393

6
7

Rattus norvegicus (Rat)
5
7

Rattus norvegicus (Rat)
5
8

Rattus norvegicus (Rat)
7
8

Rattus norvegicus (Rat)

LEUCYL-TRNA

PEROXIREDOXIN-2.

FIBRILLIN-2.

PREDICTED: SIMILAR TO

SYNTHETASE.

[MASS = 21652

[MASS = 313374

EUKARYOTIC TRANSLATION

[MASS = 134279

INITIATION FACTOR 3,

SUBUNIT 10 THETA,

150/170 KDA. [MASS = 192616

6
7

Rattus norvegicus (Rat)
5
10

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat) ACTIN,

ALPHA-2-GLOBIN

ACTIN, CYTOPLASMIC

SPLICE ISOFORM

CYTOPLASMIC 1.

CHAIN. [MASS = 15285

1. [MASS = 41737

APP770 OF AMYLOID

[MASS = 41737

BETA A4 PROTEIN

PRECURSOR

(FRAGMENT).

[MASS = 86704

6
6

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
7

Rattus norvegicus (Rat)
6
9

Rattus norvegicus (Rat)

CC2-27. [MASS = 120523

TRIOSEPHOSPHATE

HYPOTHETICAL

LUMICAN PRECURSOR.

ISOMERASE.

PROTEIN RGD1305887-

[MASS = 38279

[MASS = 26790

TUBULIN BETA CHAIN.

[MASS = 50059

6
6

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)

PREDICTED: TUMOR

LEUCYL-TRNA

QUIESCIN Q6.

PREDICTED C-1-

REJECTION ANTIGEN

SYNTHETASE.

[MASS = 82412

TETRAHYDROFOLATE

GP96. [MASS = 92771

[MASS = 134279

SYNTHASE, CYTOPLASMIC.

[MASS = 100351

6
11

Rattus norvegicus (Rat)
5
8

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)

PREDICTED: RETINOL

SPLICE ISOFORM V0 OF

Inter-alpha trypsin

VESICLE ASSOCIATED

BINDING PROTEIN 4,

VERSICAN CORE

inhibitor, heavy chain 3.

PROTEIN. [MASS = 135350

PLASMA. [MASS = 50139

PROTEIN PRECURSOR

[MASS = 98968

(FRAGMENT).

[MASS = 300008

6
7

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat) HEAT

PREDICTED:

GAMMA-GLUTAMYL

PREDICTED: SIMILAR

SHOCK COGNATE 71 KDA

TRIPARTITE MOTIF

HYDROLASE

TO PHOSPHOLIPID

PROTEIN. [MASS = 70871

PROTEIN 28.

PRECURSOR.

TRANSFER PROTEIN.

[MASS = 108785

[MASS = 35830

[MASS = 65430

6
6

Rattus norvegicus (Rat)
5
13

Rattus norvegicus (Rat)
5
7

Rattus norvegicus (Rat)
6
8

Rattus norvegicus (Rat) HEAT

PREDICTED: SIMILAR

PREDICTED: RETINOL

PREDICTED:

SHOCK PROTEIN HSP 90-

TO CELLULAR

BINDING PROTEIN 4,

HYPOTHETICAL

BETA. [MASS = 83185

APOPTOSIS

PLASMA. [MASS = 50139

PROTEIN XP_344107.

SUSCEPTIBILITY

[MASS = 189275

PROTEIN.

[MASS = 110214

6
9

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)

COLLAGEN ALPHA-1(I)

PROTEIN KINASE C-

ALPHA-1-

MATRIN-3. [MASS = 94447

CHAIN PRECURSOR.

BINDING PROTEIN

MACROGLOBULIN.

[MASS = 137886

NELL2. [MASS = 91334

[MASS = 167125

6
7

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)

ANGIOTENSINOGEN

PREDICTED: TYROSINE

PREDICTED: TYROSINE

SPLICE ISOFORM 1 OF

PRECURSOR.

KINASE RECEPTOR 1.

KINASE RECEPTOR 1.

REELIN PRECURSOR.

[MASS = 51982

[MASS = 125210

[MASS = 125210

[MASS = 387531

6
12

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
26

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

CHAPERONIN

EXTRACELLULAR

PROMININ-1S1 SPLICE

ALPHA-1B-

CONTAINING TCP1,

SUPEROXIDE

VARIANT. [MASS = 96632

GLYCOPROTEIN

SUBUNIT 2.

DISMUTASE [CU—ZN]

PRECURSOR.

[MASS = 57458

PRECURSOR.

[MASS = 56479

[MASS = 26620

6
6

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
11

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)

VALYL-TRNA

ALPHA-1-

PEPTIDYLPROLYL

CHLORIDE INTRACELLULAR

SYNTHETASE.

MACROGLOBULIN.

ISOMERASE C.

CHANNEL 6. [MASS = 64786

[MASS = 141275

[MASS32 167125

[MASS = 23009

6
7

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)

CHAPERONIN

Inter-alpha trypsin

PROTEIN KINASE C-

SPLICE ISOFORM 1 OF

CONTAINING TCP1,

inhibitor, heavy chain 3.

BINDING PROTEIN

NEURONAL CELL ADHESION

SUBUNIT 2.

[MASS = 98968

NELL2. [MASS = 91334

MOLECULE PRECURSOR.

[MASS = 57458

[MASS = 133912

6
6

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)
4
7

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)

CHAPERONIN

SPLICE ISOFORM LONG

PREDICTED:

VASCULAR CELL ADHESION

CONTAINING TCP1,

OF HYALURONAN AND

DYSTROGLYCAN 1.

PROTEIN 1 PRECURSOR.

SUBUNIT 5.

PROTEOGLYCAN LINK

[MASS = 96706

[MASS = 81246

[MASS = 59537

PROTEIN 1

PRECURSOR.

[MASS = 40262

6
9

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)

CORTICOSTEROID-

PREDICTED:

PREDICTED: SIMILAR

PROPROTEIN CONVERTASE

BINDING GLOBULIN

TRIPARTITE MOTIF

TO HEPATIC MULTIPLE

SUBTILISIN/KEXIN TYPE 9

PRECURSOR.

PROTEIN 28.

INOSITOL

PRECURSOR. [MASS = 74709

[MASS = 44672

[MASS = 108785

POLYPHOSPHATE

PHOSPHATASE.

[MASS = 54619

6
8

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat) MAMA.

60S RIBOSOMAL

STAPHYLOCOCCAL

PROMININ-1S1 SPLICE

[MASS = 63772

PROTEIN L18.

NUCLEASE DOMAIN-

VARIANT. [MASS = 96632

[MASS = 21527

CONTAINING PROTEIN

1. [MASS = 101952

6
6

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat) TLN

Neogenin precursor.

PREDICTED: MINI

EPSILON 3 GLOBIN.

PROTEIN. [MASS = 161978

[MASS = 156144

CHROMOSOME

[MASS = 16540

MAINTENANCE

DEFICIENT 6.

[MASS = 92815

6
7

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
4
9

Rattus norvegicus (Rat)
6
8

Rattus norvegicus (Rat)

TRIPEPTIDYL-

170 KDA PROTEIN-

CYSTATIN C

NESTIN. [MASS = 208797

PEPTIDASE 2.

Glutamyl-prolyl-tRNA

PRECURSOR.

[MASS = 138162

synthetase.

[MASS = 15437

[MASS = 170088

6
7

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
6
8

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

MYOSIN-10.

PREDICTED: SIMILAR

ALPHA-2-GLOBIN CHAIN.

TO LAMININ B1.

[MASS = 228965

TO PTK7 PROTEIN

[MASS = 15285

[MASS = 228429

TYROSINE KINASE 7.

[MASS = 139818

6
8

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
6
12

Rattus norvegicus (Rat)

MANNOSE 6-

ALPHA-MANNOSIDASE

GRP78 BINDING

PREDICTED: NEURAL

PHOSPHATE/INSULIN-

2. [MASS = 131242

PROTEIN.

PRECURSOR CELL

LIKE GROWTH FACTOR

[MASS = 110574

EXPRESSED,

II RECEPTOR.

DEVELOPMENTALLY DOWN-

[MASS = 273608

REGULATED GENE 4A.

[MASS = 112368

6
6

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat) 14-3-3

PREDICTED: SIMILAR

PREDICTED: NEURAL

PREDICTED: SIMILAR

PROTEIN EPSILON.

TO SMC2 PROTEIN.

PRECURSOR CELL

TO PROTOCADHERIN

[MASS = 29714

[MASS = 134280

EXPRESSED,

18 PRECURSOR.

DEVELOPMENTALLY

[MASS = 123552

DOWN-REGULATED

GENE 4A.

[MASS = 112368

6
6

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
6
7

Rattus norvegicus (Rat)

14-3-3 PROTEIN

PREDICTED: SIMILAR

M-CADHERIN.

MICROTUBULE-ASSOCIATED

EPSILON. [MASS = 29174

TO GCN1 GENERAL

[MASS = 85753

PROTEIN 4. [MASS = 110301

CONTROL OF AMINO-

ACID SYNTHESIS 1-

LIKE 1. [MASS = 302942

6
6

Rattus norvegicus (Rat)
4
7

Rattus norvegicus (Rat)
4
16

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat) FAR

PREDICTED: SIMILAR

PREDICTED:

PREDICTED: similar to

UPSTREAM ELEMENT-

TO COATOMER

DYSTROGLYCAN 1.

Fibulin-1 precursor.

BINDING PROTEIN 2.

PROTEIN COMPLEX

[MASS = 96706

[MASS = 75381

[MASS = 74226

SUBUNIT ALPHA.

[MASS = 138360

6
6

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

14-3-3 PROTEIN

AFAMIN PRECURSOR.

JUNCTION PLAKOGLOBIN.

TO CHROMATIN-

ZETA/DELTA.

[MASS = 75381

[MASS = 81801

SPECIFIC

[MASS = 27771

TRANSCRIPTION

ELONGATION FACTOR,

140 KDA SUBUNIT.

[MASS = 130435

6
6

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)

PREDICTED: KINESIN

GLUCOSE PHOSPHATE

PREDICTED: SIMILAR

PREDICTED: similar to T-

FAMILY MEMBER 4.

ISOMERASE.

TO SEMA6A PROTEIN.

complex protein 1 subunit theta.

[MASS = 139682

[MASS = 62827

[MASS = 114583

[MASS = 59745

5
8

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
4
7

Rattus norvegicus (Rat)
6
9

Rattus norvegicus (Rat)

ACTIN, CYTOPLASMIC 1.

EPSILON 1 GLOBIN.

SPLICE ISOFORM V0 OF

SERINE PEPTIDASE

[MASS = 41737

[MASS = 16105

VERSICAN CORE

INHIBITOR, CLADE F,

PROTEIN PRECURSOR

MEMBER 2. [MASS = 54893

(FRAGMENT).

[MASS = 300008

5
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat)

PREDICTED similar to C-

SPLICE ISOFORM 1 OF

PROTOCADHERIN

PREDICTED: SIMILAR TO

1-TETRAHYDROFOLATE

FIBRINOGEN ALPHA

GAMMA SUBFAMILY C,

ISOLEUCINE-TRNA

SYNTHASE,

CHAIN PRECURSOR.

3. [MASS = 101038

SYNTHETHASE.

CYTOPLASMIC.

[MASS = 86686

[MASS = 144169

[MASS = 100351

5
8

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
6
6

Rattus norvegicus (Rat) VALYL-

CATHEPSIN B

PREDICTED: similar to

SORTILIN PRECURSOR.

TRNA SYNTHETASE.

PRECURSOR.

Heterogeneous nuclear

[MASS = 91169

[MASS = 141275

[MASS = 37470

ribonucleoproteins

A2/B1. [MASS = 32468

5
6

Rattus norvegicus (Rat)
4
6

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
6
15

Rattus norvegicus (Rat)

PROTEASOME

ALPHA-2-GLOBIN

ISCHEMIA RESPONSIVE

SPLICE ISOFORM HMW OF

(PROSOME,

CHAIN. [MASS = 15285

94 KDA PROTEIN.

KININOGEN-1 PRECURSOR.

MACROPAIN) 26S

[MASS = 94057

[MASS = 70933

SUBUNIT, NON-ATPASE,

2. [MASS = 100188

5
7

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat) 14-3-3

PREDICTED: CADHERIN

PREDICTED: SIMILAR

MANNOSE 6-

PROTEIN ZETA/DELTA.

11. [MASS = 88036

TO RAN BINDING

PHOSPHATE/INSULIN-

[MASS = 27771

PROTEIN 5.

LIKE GROWTH FACTOR

[MASS = 99947

II RECEPTOR.

[MASS = 273608

5
6

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)

RAB GDP

PEPTIDYL-PROLYL CIS-

PROCOLLAGEN-

PREDICTED NUCLEOLIN-

DISSOCIATION

TRANS ISOMERASE A.

LYSINE,2-

RELATED PROTEIN NRP.

INHIBITOR BETA.

[MASS = 17743

OXOGLUTARATE 5-

[MASS = 57036

[MASS = 50685

DIOXYGENASE 3

PRECURSOR.

[MASS = 85060

5
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat) L-
4
5

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)

PREDICTED similar to

LACTATE

COMPLEMENT

PHOSPHOGLYCERATE

Nuclear autoantigenic

DEHYDROGENASE A

INHIBITORY FACTOR H.

KINASE 1. [MASS = 44423

sperm protein.

CHAIN. [MASS = 36451

[MASS = 140344

[MASS = 45764

5
5

Rattus norvegicus (Rat)
4
8

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
5
7

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: similar to

PREDICTED: SIMILAR

COLLAGEN TYPE A1(XI)7-8.

TO RIKEN CDNA

Fibulin-1 precursor.

TO LAMININ-2 ALPHA2

[MASS = 45691

B430218L07 GENE.

[MASS = 75381

CHAIN PRECURSOR.

[MASS = 143906

[MASS = 359007

5
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
4
6

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat) RAT T-

LAR RECEPTOR-LINKED

PROTEASOME

TENASCIN

KININOGEN. [MASS = 47618

TYROSINE

(PROSOME,

(FRAGMENT).

PHOSPHATASE.

MACROPAIN) 26S

[MASS = 67815

[MASS = 181130

SUBUNIT, NON-ATPASE,

2. [MASS = 100188

5
7

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
4
6

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)

SPLICE ISOFORM

AFAMIN PRECURSOR.

FIBULIN-2 ISOFORM A.

PREDICTED: SIMILAR TO

GAMMA-B OF

[MASS = 69335

[MASS = 126193

IMPORTIN 9. [MASS = 131739

FIBRINOGEN GAMMA

CHAIN PRECURSOR.

[MASS = 50633

5
5

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)

ADENOSYLHOMO-

SPARC-LIKE PROTEIN 1

PREDICTED: SIMILAR

COMPLEMENT C4

CYSTEINASE.

PRECURSOR.

TO COLLAGEN ALPHA1

PRECURSOR. [MASS = 192163

[MASS = 47407

[MASS = 70634

TYPE VI-PRECURSOR.

[MASS = 130760

5
5

Rattus norvegicus (Rat)
4
10

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

SERINE PEPTIDASE

PROTHROMBIN

NONO/P54NRB HOMOLOG.

PROTEIN SET.

INHIBITOR, CLADE F,

PRECURSOR

[MASS = 75487

[MASS = 33406

MEMBER 2.

(FRAGMENT).

[MASS = 54893

[MASS = 70412

5
5

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)

IRON-RESPONSIVE

SORTILIN PRECURSOR.

TRANSCOBALAMIN-2

KALLISTATIN. [MASS = 48021

ELEMENT-BINDING

[MASS = 91169

PRECURSOR.

PROTEIN 1.

[MASS = 47420

[MASS = 98128

5
6

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED:

PREDICTED: SIMILAR

EXPORTIN-1. [MASS = 123335

TO EUKARYOTIC

AMINOPEPTIDASE

TO VINCULIN.

TRANSLATION

PUROMYCIN

[MASS = 116615

INITIATION FACTOR 3,

SENSITIVE.

SUBUNIT 10 THETA,

[MASS = 103344

150/170 KDA.

[MASS = 192616

5
9

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
5
8

Rattus norvegicus (Rat)

SERINE PEPTIDASE

PREDICTED: SIMILAR

PREDICTED: SIMILAR

PREDICTED-INHIBIN BINDING

INHIBITOR, CLADE F,

TO PROTOCADHERIN 1

TO PLEXIN-B2

PROTEIN LONG ISOFORM.

MEMBER 2.

ISOFORM 2

PRECURSOR.

[MASS = 153224

[MASS = 54893

PRECURSOR.

[MASS = 216119

[MASS = 138914

5
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
10

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

EXPORTIN-1.

14-3-3 PROTEIN

CATHEPSIN B PRECURSOR.

TO SEROTRANSFERRIN

[MASS = 123335

ZETA/DELTA.

[MASS = 37470

PRECURSOR.

[MASS = 27771

[MASS = 76607

5
7

Rattus norvegicus (Rat)
4
6

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
8

Rattus norvegicus (Rat)

COLLAGEN ALPHA-1(III)

SPLICE ISOFORM HMW

COLLAGEN TYPE

PREDICTED: SIMILAR TO

CHAIN PRECURSOR.

OF KININOGEN-1

A1(XI)7-8. [MASS = 45691

RAN BINDING PROTEIN 5.

[MASS = 138936

PRECURSOR.

[MASS = 133476

[MASS = 70933

5
8

Rattus norvegicus (Rat)
4
7

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
16

Rattus norvegicus (Rat)

TENASCIN

GLUTATHIONE

PROTECTIVE PROTEIN

CYSTATIN C PRECURSOR.

(FRAGMENT).

PEROXIDASE 3

FOR BETA-

[MASS = 15437

[MASS = 62473

PRECURSOR.

GALACTOSIDASE.

[MASS = 25393

[MASS = 51216

5
7

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
5
7

Rattus norvegicus (Rat)

NUCLEOSOME

PREDICTED: SIMILAR

CREATINE KINASE B-

LACTADHERIN PRECURSOR.

ASSEMBLY PROTEIN 1-

TO PTK7 PROTEIN

TYPE. [MASS = 42712

[MASS = 47413

LIKE 1. [MASS = 45373

TYROSINE KINASE 7.

[MASS = 139818

5
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)

PREDICTED: VON

SPLICE ISOFORM 1 OF

PEROXIREDOXIN-2.

SPLICE ISOFORM GAMMA-B

WILLEBRAND FACTOR.

SEX HORMONE-

[MASS = 21652

OF FIBRINOGEN GAMMA

[MASS = 308474

BINDING GLOBULIN

CHAIN PRECURSOR.

PRECURSOR.

[MASS = 50633

[MASS = 44533

5
5

Rattus norvegicus (Rat)
4
6

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PEPTIDYLPROLYL

PREDICTED: SIMILAR

EPSILON 1 GLOBIN.

TO HEPATIC MULTIPLE

ISOMERASE C.

TO PROTOCADHERIN 1

[MASS = 16105

INOSITOL

[MASS = 23009

ISOFORM 2

POLYPHOSPHATE

PRECURSOR.

PHOSPHATASE.

[MASS = 138914

[MASS = 54619

5
6

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)

40S RIBOSOMAL

QUIESCIN Q6.

GAMMA-GLUTAMYL

TRIPEPTIDYL-PEPTIDASE 2.

PROTEIN SA.

[MASS = 82412

HYDROLASE

[MASS = 138162

[MASS = 32693

PRECURSOR.

[MASS = 35830

5
5

Rattus norvegicus (Rat)
4
6

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
7

Rattus norvegicus (Rat)

TRIOSEPHOSPHATE

SPLICE ISOFORM 1 OF

PREDICTED: similar to

PREDICTED: SIMILAR TO

ISOMERASE.

HETEROGENEOUS

Slit-like 2. [MASS = 72321

CRB2 PROTEIN.

[MASS = 26790

NUCLEAR

[MASS = 138781

RIBONUCLEOPROTEIN

D0. [MASS = 38192

5
6

Rattus norvegicus (Rat)
4
6

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)

HEMOPEXIN

PREDICTED: SIMILAR

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

PRECURSOR.

TO PHOSPHOLIPID

TO CRB2 PROTEIN.

ALANYL-TRNA SYNTHETASE.

[MASS = 51291

TRANSFER PROTEIN.

[MASS = 138781

[MASS = 106811

[MASS = 65430

5
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

HEAT SHOCK PROTEIN

ACTIN, CYTOPLASMIC

PREDICTED: MINI

TO CCTETA, ETA

HSP 90-BETA.

1. [MASS = 41737

CHROMOSOME

SUBUNIT OF THE

[MASS = 83185

MAINTENANCE DEFICIENT 4

CHAPERONIN

HOMOLOG. [MASS = 96685

CONTAINING TCP-1.

[MASS = 75684

5
7

Rattus norvegicus (Rat)
4
6

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat)

PROFILIN-1.

LOW-DENSITY

LOW-DENSITY

PREDICTED: SIMILAR TO

[MASS = 14826

LIPOPROTEIN

LIPOPROTEIN

VINCULIN. [MASS = 116615

RECEPTOR

RECEPTOR

PRECURSOR.

PRECURSOR.

[MASS = 96622

[MASS = 96622

5
6

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

BETA-2-

PREDICTED: SIMILAR

SPLICE ISOFORM 1 OF

NEURONAL CELL

MICROGLOBULIN

TO LERK-5.

ATTRACTIN PRECURSOR.

ADHESION MOLECULE

PRECURSOR.

[MASS = 37282

[MASS = 163296

PRECURSOR.

[MASS = 13720

[MASS = 133912

5
5

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
5
7

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

FETUB PROTEIN.

HEPATOCYTE GROWTH

GLUCOSE PHOSPHATE

TO FRAS1 RELATED

[MASS = 43169

FACTOR ACTIVATOR.

ISOMERASE. [MASS = 62827

EXTRACELLULAR

[MASS = 70737

MATRIX PROTEIN 2.

[MASS = 378709

5
8

Rattus norvegicus (Rat) T-
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat)

COMPLEX PROTEIN 1

PREDICTED: SIMILAR

MANNOSIDASE 2,

SPLICE ISOFORM 2 OF

SUBUNIT DELTA.

TO PROTOCADHERIN

ALPHA B1.

RECEPTOR-TYPE TYROSINE-

[MASS = 57968

18 PRECURSOR.

[MASS = 114327

PROTEIN PHOSPHATASE

[MASS = 123552

ZETA PRECURSOR.

[MASS = 164596

5
6

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
5

Rattus norvegicus (Rat) HEAT-

SPLICE ISOFORM HMW

COMPLEMENT

NEUROPILIN-2

SHOCK PROTEIN 105 KDA.

OF KININOGEN-1

COMPONENT 2.

PRECURSOR.

[MASS = 96419

PRECURSOR.

[MASS = 83699

[MASS = 104473

[MASS = 70933

5
8

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
7

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR

PROCOLLAGEN C-

PREDICTED similar to Nuclear

TO RIBOSOMAL

TO HEPATIC MULTIPLE

ENDOPEPTIDASE

autoantigenic sperm protein.

PROTEIN L6.

INOSITOL

ENHANCER 1

[MASS = 45764

[MASS = 32944

POLYPHOSPHATE

PRECURSOR.

PHOSPHATASE.

[MASS = 50185

[MASS = 54619

5
6

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
5
6

Rattus norvegicus (Rat) LAR

60S RIBOSOMAL

NESTIN. [MASS = 208797

FATTY ACID SYNTHASE.

RECEPTOR-LINKED

PROTEIN L4.

[MASS = 272650

TYROSINE PHOSPHATASE.

[MASS = 47126

[MASS = 181130

4
4

Rattus norvegicus (Rat) IG
4
4

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat) IG

KAPPA CHAIN C

SPLICE ISOFORM 2 OF

PEPTIDYL-PROLYL CIS-

KAPPA CHAIN C REGION, B

REGION, B ALLELE.

RECEPTOR-TYPE

TRANS ISOMERASE A.

ALLELE. [MASS = 11601

[MASS = 11601

TYROSINE-PROTEIN

[MASS = 17743

PHOSPHATASE ZETA

PRECURSOR.

[MASS = 164596

4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)

EUKARYOTIC

CULLIN-ASSOCIATED

SPLICE ISOFORM 1 OF

CHAPERONIN CONTAINING

TRANSLATION

NEDD8-DISSOCIATED

SEX HORMONE-

TCP1, SUBUNIT 2.

INITIATION FACTOR

PROTEIN 1.

BINDING GLOBULIN

[MASS = 57458

4A2. [MASS = 46489

[MASS = 136362

PRECURSOR.

[MASS = 44533

4
7

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

HNRPK PROTEIN.

PREDICTED: SIMILAR

CELL GROWTH

PREDICTED: TUMOR

[MASS = 51028

TO PROTOCADHERIN

REGULATOR WITH EF

REJECTION ANTIGEN GP96.

19 PRECURSOR.

HAND DOMAIN 1.

[MASS = 92771

[MASS = 125989

[MASS = 30835

4
5

Rattus norvegicus (Rat)
4
9

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

COLLAGEN ALPHA-1(III)

ROUNDABOUT

DREBRIN 1. [MASS = 77472

TO RAN BINDING

CHAIN PRECURSOR.

HOMOLOG 1

PROTEIN 5.

[MASS = 138936

PRECURSOR.

[MASS = 133476

[MASS = 180748

4
5

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

EPSILON 3 GLOBIN.

BETA-2-

PREDICTED: SIMILAR TO

TO RAN BINDING

[MASS = 16540

MICROGLOBULIN

DNA REPLICATION

PROTEIN 5.

PRECURSOR.

LICENSING FACTOR MCM3.

[MASS = 99947

[MASS = 13720

[MASS = 83429

4
5

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
3
6

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)

RATSG1. [MASS = 49199

MANNOSE 6-

FOLLISTATIN-RELATED

PREDICTED: SIMILAR TO

PHOSPHATE/INSULIN-

PROTEIN 1

IMPORTIN 7. [MASS = 119704

LIKE GROWTH FACTOR

PRECURSOR.

II RECEPTOR.

[MASS = 34622

[MASS = 273608

4
9

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

HEAT SHOCK PROTEIN

PREDICTED

CLUSTERIN

PREDICTED: SIMILAR TO

HSP 90-BETA.

NUCLEOLIN-RELATED

PRECURSOR.

RANBP21. [MASS = 136714

[MASS = 83185

PROTEIN NRP.

[MASS = 51375

[MASS = 57036

4
6

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

PEPTIDYL-PROLYL CIS-

DYNEIN HEAVY CHAIN,

PROTEIN DISULFIDE-

ALPHA-ACTININ-1.

TRANS ISOMERASE A.

CYTOSOLIC.

ISOMERASE

[MASS = 102960

[MASS = 17743

[MASS = 532252

PRECURSOR.

[MASS = 56951

4
6

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

PEROXIREDOXIN-2.

PEROXIREDOXIN-1.

PREDICTED: SIMILAR

ARCADLIN. [MASS = 103800

[MASS = 21652

[MASS = 22109

TO INTER-ALPHA-

INHIBITOR H2 CHAIN.

[MASS = 105715

4
7

Rattus norvegicus (Rat)
4
22

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

EXTRACELLULAR

PREDICTED: SIMILAR

PROTOCADHERIN GAMMA

HETEROGENEOUS

SUPEROXIDE

TO ELASTIN

SUBFAMILY C, 3.

NUCLEAR

DISMUTASE [CU-ZN]

MICROFIBRIL

[MASS = 101038

RIBONUCLEOPROTEIN

PRECURSOR.

INTERFACER 1.

D0. [MASS = 38192

[MASS = 26620

[MASS = 107560

4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

PREDICTED: similar to

PREDICTED: SIMILAR

PREDICTED: SIMILAR

EUKARYOTIC TRANSLATION

Heterogeneous nuclear

TO FILAMIN A.

TO ALPHA 2 TYPE VI

INITIATION FACTOR 4A,

ribonucleoproteins

[MASS = 290169

COLLAGEN ISOFORM

ISOFORM 1. [MASS = 46154

A2/B1. [MASS = 32468

2C2A PRECURSOR.

[MASS = 98272

4
4

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

40S RIBOSOMAL

COMPLEMENT

PREDICTED: SIMILAR

RIBONUCLEOTIDE

PROTEIN S3A.

INHIBITORY FACTOR H.

TO COLLAGEN ALPHA

REDUCTASE M1.

[MASS = 29814

[MASS = 140344

2(IV) CHAIN

[MASS = 90293

PRECURSOR - MOUSE.

[MASS = 192535

4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
7

Rattus norvegicus (Rat)

TXNRD1 PROTEIN.

PREDICTED: KINESIN

SPLICE ISOFORM 1 OF

PREDICTED: RETINOL

[MASS = 63002

FAMILY MEMBER 4.

NEUROFASCIN

BINDING PROTEIN 4,

[MASS = 139682

PRECURSOR.

PLASMA. [MASS = 50139

[MASS = 138004

4
5

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

EUKARYOTIC

CELL GROWTH

SERINE (OR CYSTEINE)

PREDICTED: COMPLEMENT

TRANSLATION

REGULATOR WITH EF

PROTEINASE

COMPONENT 7.

INITIATION FACTOR 4A,

HAND DOMAIN 1.

INHIBITOR, CLADE A

[MASS = 90661

ISOFORM 1.

[MASS = 30835

(ALPHA-1

[MASS = 46154

ANTIPROTEINASE,

ANTITRYPSIN),

MEMBER 6.

[MASS = 44671

4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
6

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)

14-3-3 PROTEIN THETA.

LEUKEMIA INHIBITORY

SP120-Heterogeneous

PREDICTED: SIMILAR TO

[MASS = 27778

FACTOR RECEPTOR

nuclear ribonucleoprotein

CCTETA, ETA SUBUNIT OF

PRECURSOR.

U. [MASS = 87748

THE CHAPERONIN

[MASS = 122394

CONTAINING TCP-1.

[MASS = 75684

4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
6

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

ALPHA ISOFORM OF

PREDICTED: SIMILAR

RIBOSOMAL PROTEIN

PREDICTED: COMPLEMENT

REGULATORY SUBUNIT

TO PLEXIN-B2

S27A. [MASS = 17951

COMPONENT 5.

A, PROTEIN

PRECURSOR.

[MASS = 152144

PHOSPHATASE 2.

[MASS = 216119

[MASS = 65323

4
4

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
4
6

Rattus norvegicus (Rat)

PROTEIN KINASE C-

LOW-DENSITY

SPLICE ISOFORM 2 OF

NEUROCAN CORE PROTEIN

BINDING PROTEIN

LIPOPROTEIN

RECEPTOR-TYPE

PRECURSOR. [MASS = 135545

NELL2. [MASS = 91334

RECEPTOR-RELATED

TYROSINE-PROTEIN

PROTEIN 2

PHOSPHATASE ZETA

PRECURSOR.

PRECURSOR.

[MASS = 519276

[MASS = 164596

4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)

STRUCTURAL

LEUKOCYTE COMMON

60S RIBOSOMAL

PREDICTED: SIMILAR TO

MAINTENANCE OF

ANTIGEN-RELATED

PROTEIN L13.

SEIZURE 6-LIKE PROTEIN

CHROMOSOME 3.

PHOSPHATASE

[MASS = 24178

PRECURSOR. [MASS = 145870

[MASS = 138448

PRECURSOR.

[MASS = 212954

4
4

Rattus norvegicus (Rat)
4
7

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PROTOCADHERIN

PREDICTED:

HEPARIN COFACTOR 2

TO IMPORTIN 7.

GAMMA SUBFAMILY C,

COMPLEMENT

PRECURSOR. [MASS = 54552

[MASS = 119704

3. [MASS = 101038

COMPONENT 7.

[MASS = 90661

4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

CADHERIN-6

MYOSIN-9.

VIMENTIN.

PREDICTED: SIMILAR TO

PRECURSOR.

[MASS = 226207

[MASS = 53602

DNA REPLICATION

[MASS = 88341

LICENSING FACTOR MCM2.

[MASS = 102272

4
7

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)

VITAMIN D-BINDING

14-3-3 PROTEIN

LEUKOCYTE COMMON

PREDICTED: NIDOGEN.

PROTEIN PRECURSOR.

EPSILON. [MASS = 29174

ANTIGEN-RELATED

[MASS = 138365

[MASS = 53544

PHOSPHATASE

PRECURSOR.

[MASS = 207012

4
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
7

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

THROMBOSPONDIN 1.

NEUROSERPIN

PEPTIDYLPROLYL

TO ALANYL-TRNA

[MASS = 129671

PRECURSOR.

ISOMERASE C. [MASS = 23009

SYNTHETASE.

[MASS = 46278

[MASS = 106811

4
4

Rattus norvegicus (Rat)
4
7

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

PREDICTED:

HISTONE H1.2.

NEUROCAN CORE

SPLICE ISOFORM 2 OF

TRANSFORMING

[MASS = 21856

PROTEIN PRECURSOR.

POLYPYRIMIDINE TRACT-

GROWTH FACTOR,

[MASS = 135545

BINDING PROTEIN 2.

BETA INDUCED, 68 KDA.

[MASS = 57645

[MASS = 74369

4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

PREDICTED: similar to

RATSG1. [MASS = 49199

PREDICTED: SIMILAR

TXNRD1 PROTEIN.

phosphoribosylformyl-

TO AMYLOID BETA (A4)

[MASS = 63002

glycinamidine

PRECURSOR-LIKE

synthase.

PROTEIN 1.

[MASS = 146178

[MASS = 68777

4
6

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

CC2-27. [MASS = 120523

HEPARIN COFACTOR 2

TENASCIN (FRAGMENT).

TO CRB2 PROTEIN.

PRECURSOR.

[MASS = 62473

[MASS = 138781

[MASS = 54552

4
4

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
5

Rattus norvegicus (Rat)

AFAMIN PRECURSOR.

PREDICTED: SIMILAR

NETRIN RECEPTOR

QUIESCIN Q6. [MASS = 82412

[MASS = 69335

TO ALPHA ENOLASE.

UNC5C PRECURSOR.

[MASS = 46489

[MASS = 103135

4
4

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
6

Rattus norvegicus (Rat) POLY

PREDICTED: SIMILAR

HYRAC. [MASS = 31353

CULLIN-ASSOCIATED

[ADP-RIBOSE] POLYMERASE

TO RANBP21.

NEDD8-DISSOCIATED

1. [MASS = 112529

[MASS = 136714

PROTEIN 1.

[MASS = 136362

4
6

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

BETA-2-

VITAMIN D-BINDING

LEUKEMIA INHIBITORY

SPLICE ISOFORM 1 OF

MICROGLOBULIN

PROTEIN PRECURSOR.

FACTOR RECEPTOR

CULLIN-ASSOCIATED

PRECURSOR.

[MASS = 53544

PRECURSOR.

NEDD8-DISSOCIATED

[MASS = 13720

[MASS = 122394

PROTEIN 2. [MASS = 139673

4
6

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

60S RIBOSOMAL

EUKARYOTIC

CLATHRIN HEAVY

PREDICTED: SIMILAR TO

PROTEIN L13.

TRANSLATION

CHAIN. [MASS = 191599

INTER-ALPHA TRYPSIN

[MASS = 24178

INITIATION FACTOR

INHIBITOR, HEAVY CHAIN 1.

4A2. [MASS = 46489

[MASS = 104581

4
6

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

40S RIBOSOMAL

PREDICTED: similar to

FAM3C-LIKE PROTEIN.

MANNOSIDASE 2, ALPHA B1.

PROTEIN S6.

phosphoribosylformylglycinamidine

[MASS = 24714

[MASS = 114327

[MASS = 28681

synthase.

[MASS = 146178

4
5

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

14-3-3 PROTEIN

FOLLISTATIN-RELATED

PREDICTED: SIMILAR

STRUCTURAL

GAMMA. [MASS = 28171

PROTEIN 1

TO LAMININ ALPHA-1

MAINTENANCE OF

PRECURSOR.

CHAIN PRECURSOR -

CHROMOSOME 3.

[MASS = 34622

MOUSE. [MASS = 338692

[MASS = 138448

4
6

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

40S RIBOSOMAL

THROMBOSPONDIN 1.

DIHYDROPYRIMIDINASE-

TO PROGRAMMED CELL

PROTEIN SA.

[MASS = 129671

RELATED PROTEIN 2.

DEATH 6 INTERACTING

[MASS = 32693

[MASS = 62278

PROTEIN. [MASS = 75806

4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

14-3-3 PROTEIN THETA.

MANNOSIDASE, ALPHA,

PREDICTED: SIMILAR TO P30

TO PUTATIVE PRE-

[MASS = 27778

CLASS A1, MEMBER 1.

DBC PROTEIN.

MRNA SPLICING

[MASS = 73125

[MASS = 114440

FACTOR RNA

HELICASE.

[MASS = 90977

4
15

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat) 26S

EXTRACELLULAR

60S RIBOSOMAL

HYRAC. [MASS = 31353

PROTEASOME NON-ATPASE

SUPEROXIDE

PROTEIN L18.

REGULATORY SUBUNIT 1.

DISMUTASE [CU—ZN]

[MASS = 21527

[MASS = 105748

PRECURSOR.

[MASS = 26620

4
4

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

SMC4L1 PROTEIN.

ADENOSYLHOMOCYSTEINASE.

SEZ6B. [MASS = 105607

FAM3C-LIME PROTEIN.

[MASS = 146806

[MASS = 47407

[MASS = 24714

4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

SPLICEOSOME RNA

SPARC PRECURSOR.

IG KAPPA CHAIN C

LEUKEMIA INHIBITORY

HELICASE BAT1.

[MASS = 34384

REGION, B ALLELE.

FACTOR RECEPTOR

[MASS = 49035

[MASS = 11601

PRECURSOR. [MASS = 122394

4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PROCOLLAGEN-

EUKARYOTIC

HAUSP. [MASS = 128431

TO 40S RIBOSOMAL

LYSINE,2-

TRANSLATION

PROTEIN S9.

OXOGLUTARATE 5-

INITIATION FACTOR

[MASS = 22648

DIOXYGENASE 3

4A2. [MASS = 46489

PRECURSOR.

[MASS = 85060

4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
4
4

Rattus norvegicus (Rat)

60S ACIDIC RIBOSOMAL

PREDICTED similar to C-

PREDICTED: SIMILAR

COLLAGEN ALPHA-1(I)

PROTEIN P0.

1-TETRAHYDROFOLATE

TO CG1841-PA,

CHAIN PRECURSOR.

[MASS = 34215

SYNTHASE,

ISOFORM A.

[MASS = 137886

CYTOPLASMIC.

[MASS = 52522

[MASS = 100351

4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
4
6

Rattus norvegicus (Rat) FETUB

PREDICTED: SIMILAR

M-CADHERIN.

ALPHA-2-HS-

PROTEIN. [MASS = 43169

TO P30 DBC PROTEIN.

[MASS = 85753

GLYCOPROTEIN

[MASS = 11440

PRECURSOR.

[MASS = 37982

4
4

Rattus norvegicus (Rat)
3
11

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

RIBOSOMAL PROTEIN

VITAMIN D-BINDING

PROTEASOME SUBUNIT

TO ISOLEUCINE-TRNA

S27A. [MASS = 17951

PROTEIN PRECURSOR.

ALPHA TYPE 2. [MASS = 25795

SYNTHETASE.

[MASS = 53544

[MASS = 144169

4
5

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

40S RIBOSOMAL

PREDICTED: SIMILAR

VACUOLAR ATP

RATSG1. [MASS = 49199

PROTEIN S10.

TO ALPHA NAC/1.9.2.

SYNTHASE SUBUNIT S1

[MASS = 18916

PROTEIN. [MASS = 23384

PRECURSOR.

[MASS = 51123

4
4

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

SPLICE ISOFORM 1 OF

PREDICTED: ATPASE,

COFILIN-1. [MASS = 24588

TO VINCULIN.

ALPHA-1B-

H+ TRANSPORTING,

[MASS = 116615

GLYCOPROTEIN

LYSOSOMAL

PRECURSOR.

ACCESSORY PROTEIN

[MASS = 56479

2. [MASS = 66094

4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)

PREDICTED:

SUPEROXIDE

PREDICTED: SIMILAR

PEPTIDYL-PROLYL CIS-

CALSYNTENIN 1.

DISMUTASE.

TO PROCOLLAGEN,

TRANS ISOMERASE A.

[MASS = 109351

[MASS = 15780

TYPE IX, ALPHA 2.

[MASS = 17743

[MASS = 71422

4
4

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat) RAB

FAR UPSTREAM

EUKARYOTIC

PREDICTED: SIMILAR

GDP DISSOCIATION

ELEMENT-BINDING

TRANSLATION

TO ALPHA ENOLASE.

INHIBITOR BETA.

PROTEIN 2.

INITIATION FACTOR 4A,

[MASS = 46489

[MASS = 50685

[MASS = 74226

ISOFORM 1.

[MASS = 46154

4
5

Rattus norvegicus (Rat) L-
3
5

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat) 40S

LACTATE

COLLAGEN ALPHA-1(V)

SOLUBLE CALCIUM-

RIBOSOMAL PROTEIN SA.

DEHYDROGENASE A

CHAIN PRECURSOR.

ACTIVATED

[MASS = 32693

CHAIN. [MASS = 36451

[MASS = 184610

NUCLEOTIDASE 1.

[MASS = 45659

4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

NONO/P54NRB

VASCULAR CELL

PHOSPHATIDY-

ACETYL-COA

HOMOLOG.

ADHESION PROTEIN 1

LETHANOLAMINE-

CARBOXYLASE 1.

[MASS = 75487

PRECURSOR.

BINDING PROTEIN.

[MASS = 265421

[MASS = 81246

[MASS = 20670

4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

MICROTUBULE-

PREDICTED:

PREDICTED: similar to

SECRETOGRANIN-3

ASSOCIATED PROTEIN

CALSYNTENIN 1.

alpha 1 type II collagen.

PRECURSOR. [MASS = 53183

4. [MASS = 110301

[MASS = 109351

[MASS = 138706

4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)

COLD SHOCK DOMAIN-

RUVB-LIKE 1.

C-REACTIVE PROTEIN

PEROXIREDOXIN-2

CONTAINING PROTEIN

[MASS = 50214

PRECURSOR.

[MASS = 21652

E1. [MASS = 88895

[MASS = 25468

4
4

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)

26S PROTEASOME

PREDICTED similar to

EPITHELIAL-CADHERIN

SERINE/CYSTEINE

NON-ATPASE

POSTSYNAPTIC

PRECURSOR.

PROTEINASE INHIBITOR,

REGULATORY SUBUNIT

DENSITY PROTEIN.

[MASS = 98715

CLADE C, MEMBER 1.

1. [MASS = 105748

[MASS = 186848

[MASS = 52234

4
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

60S RIBOSOMAL

BIFUNCTIONAL

PREDICTED: SIMILAR TO

TO RAS GTPASE-

PROTEIN L4.

HEPARAN SULFATE N-

AMYLOID BETA (A4)

ACTIVATING-LIKE

[MASS = 47126

DEACETYLASE/N-

PRECURSOR-LIKE PROTEIN

PROTEIN IQGAP1.

SULFOTRANSFERASE 1

1. [MASS = 68777

[MASS = 196522

(EC 2.8.2.8)

(GLUCOSAMINYL N-

DEACETYLASE/N-

SULFOTRANSFERASE

1) (NDST-1) (.

[MASS = 101202

4
5

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)

GTP-BINDING NUCLEAR

PROTEIN DISULFIDE-

SPARC PRECURSOR.

PREDICTED: SIMILAR TO

PROTEIN RAN, TESTIS-

ISOMERASE

[MASS = 34384

RAN BINDING PROTEIN 5.

SPECIFIC ISOFORM.

PRECURSOR.

[MASS = 99947

[MASS = 24451

[MASS = 56951

3
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

14-3-3 PROTEIN

PREDICTED: SIMILAR

PREDICTED:

PREDICTED: SIMILAR TO

ZETA/DELTA.

TO LAMININ-2 ALPHA2

GLYCOPROTEIN-4-

PROGRAMMED CELL DEATH

[MASS = 27771

CHAIN PRECURSOR.

BETA-

6 INTERACTING PROTEIN.

[MASS = 359007

GALACTOSYL-

[MASS = 75806

TRANSFERASE

2. [MASS = 44484

3
5

Rattus norvegicus (Rat)
3
6

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
6

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR

PREDICTED: SIMILAR

PREDICTED:

TO ALPHA ENOLASE.

TO INTER-ALPHA-

TO PAPPALYSIN-2

DYSTROGLYCAN 1.

[MASS = 46489

INHIBITOR H2 CHAIN.

PRECURSOR.

[MASS = 96706

[MASS = 105715

[MASS = 204770

3
5

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

GLUTATHIONE

SHEN-DAN.

MASP-3 PROTEIN.

PREDICTED: similar to Slit-like

PEROXIDASE 3

[MASS = 131080

[MASS = 82497

2. [MASS = 72321

PRECURSOR.

[MASS = 25393

3
6

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

PREDICTED:

FAR UPSTREAM

PREDICTED: similar to T-

SPLICE ISOFORM 1 OF

DYSTROGLYCAN 1.

ELEMENT-BINDING

complex protein 1 subunit

HETEROGENEOUS

[MASS = 96706

PROTEIN 2.

theta. [MASS = 59745

NUCLEAR

[MASS = 74226

RIBONUCLEOPROTEIN D0.

[MASS = 38192

3
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

COFILIN-1.

PREDICTED: SIMILAR

NUCLEAR

NEURAL CELL ADHESION

[MASS = 24588

TO IMPORTIN 9.

AUTOANTIGENIC

MOLECULE 1, 140 KDA

[MASS = 131739

SPERM PROTEIN.

ISOFORM PRECURSOR.

[MASS = 84200

[MASS = 94658

3
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

HEAT-SHOCK PROTEIN

PREDICTED: SIMILAR

SUPEROXIDE

PREDICTED: SIMILAR TO

105 KDA. [MASS = 96419

TO VINCULIN.

DISMUTASE.

PUTATIVE PRE-MRNA

[MASS = 116615

[MASS = 15780

SPLICING FACTOR RNA

HELICASE. [MASS = 90977

3
4

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat) DNA

PREDICTED: SIMILAR

PREDICTED similar to

APOLIPOPROTEIN D

POLYMERASE ALPHA

TO ALPHA NAC/1.9.2.

Nuclear autoantigenic

PRECURSOR.

CATALYTIC SUBUNIT

PROTEIN. [MASS = 23384

sperm protein.

[MASS = 21635

(FRAGMENT). [MASS = 165306

[MASS = 45764

3
7

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

TUMOR NECROSIS

FAM3C-LIKE PROTEIN.

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

FACTOR TYPE 1

[MASS = 24714

TO PROGRAMMED

ARX. [MASS = 121446

RECEPTOR

CELL DEATH 6

ASSOCIATED PROTEIN.

INTERACTING

[MASS = 80461

PROTEIN. [MASS = 75806

3
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)

FRUCTOSE-

D-3-

PREDICTED: SIMILAR

FOLLISTATIN-RELATED

BISPHOSPHATE

PHOSPHOGLYCERATE

TO SEMAPHORIN 6D-4.

PROTEIN 1 PRECURSOR.

ALDOLASE A.

DEHYDROGENASE.

[MASS = 159473

[MASS = 34622

[MASS = 39221

[MASS = 56362

3
3

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)
2
4

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)

PREDICTED: BRAIN

PREDICTED: SIMILAR

IGH-1A PROTEIN.

SPLICE ISOFORM PYBP1 OF

GLYCOGEN

TO COLLAGEN ALPHA

[MASS = 51403

POLYPYRIMIDINE TRACT-

PHOSPHORYLASE.

2(IV) CHAIN

BINDING PROTEIN 1.

[MASS = 96738

PRECURSOR - MOUSE.

[MASS = 56937

[MASS = 192535

3
7

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat) L-
3
4

Rattus norvegicus (Rat)

CYSTATIN C

PROLIFERATION-

LACTATE

PREDICTED: SIMILAR TO

PRECURSOR.

ASSOCIATED 2G4,

DEHYDROGENASE A

HEAT SHOCK PROTEIN HSP

[MASS = 15437

38 KDA. [MASS = 43657

CHAIN. [MASS = 36451

90-BETA. [MASS = 80701

3
3

Rattus norvegicus (Rat)
3
6

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

14-3-3 PROTEIN

RECEPTOR-LIKE

PREDICTED: SIMILAR TO

TO PTK7 PROTEIN

GAMMA. [MASS = 28171

PROTEIN TYROSINE

DNA REPLICATION

TYROSINE KINASE 7.

PHOSPHATASE KAPPA

LICENSING FACTOR MCM5.

[MASS = 139818

EXTRACELLULAR

[MASS = 91577

REGION. [MASS = 56159

3
10

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)

RIBOSOMAL PROTEIN

PREDICTED: SIMILAR

PREDICTED: SIMILAR

PROFILIN-1. [MASS = 14826

S27A. [MASS = 17951

TO RAS GTPASE-

TO SEROTRANSFERRIN

ACTIVATING-LIKE

PRECURSOR.

PROTEIN IQGAP1.

[MASS = 76607

[MASS = 196522

3
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

COMPLEMENT C4

PROTOCADHERIN.

PREDICTED: SIMILAR

PREDICTED: CALSYNTENIN

PRECURSOR.

[MASS = 505997

TO RAN BINDING

1. [MASS = 109351

[MASS = 192163

PROTEIN 5.

[MASS = 133476

3
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat) 60S

POLY [ADP-RIBOSE]

CHAPERONIN SUBUNIT

APOLIPOPROTEIN M

RIBOSOMAL PROTEIN L18.

POLYMERASE 1.

6A. [MASS = 58017

PRECURSOR.

[MASS = 21527

[MASS = 112529

[MASS = 21513

3
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

40S RIBOSOMAL

PREDICTED: SIMILAR

HEAT SHOCK PROTEIN

PREDICTED: SIMILAR TO

PROTEIN S4, X

TO EUKARYOTIC

HSP 90-BETA.

SPLICING FACTOR 3B,

ISOFORM. [MASS = 29467

TRANSLATION

[MASS = 83185

SUBUNIT 3, 130 KDA.

INITIATION FACTOR 3,

[MASS = 174174

SUBUNIT 10 THETA,

150/170 KDA.

[MASS = 192616

3
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

40S RIBOSOMAL

PREDICTED: SIMILAR

CATHEPSIN D

SPARC-LIKE PROTEIN 1

PROTEIN S8.

TO VERY LARGE G

PRECURSOR.

PRECURSOR. [MASS = 70634

[MASS = 24074

PROTEIN-COUPLED

[MASS = 44681

RECEPTOR 1.

[MASS = 413840

3
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

EPSILON 3 GLOBIN.

CADHERIN EGF LAG

MICROFIBRILLAR-

EUKARYOTIC TRANSLATION

[MASS = 16540

SEVEN-PASS G-TYPE

ASSOCIATED PROTEIN

INITIATION FACTOR 4A2.

RECEPTOR 2.

4. [MASS = 29050

[MASS = 46489

[MASS = 317122

3
3

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)

PREDICTED:

STATHMIN.

FRUCTOSE-

EXTRACELLULAR

PHOSPHORIBOSYL-

[MASS = 17157

BISPHOSPHATE

SUPEROXIDE DISMUTASE

GLYCINAMIDE

ALDOLASE A.

[CU—ZN] PRECURSOR.

FORMYLTRANSFERASE.

[MASS = 39221

[MASS = 26620

[MASS = 107580

3
6

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

GTP-BINDING NUCLEAR

ADAMTS-1

CADHERIN-6 PRECURSOR.

TO HEAT SHOCK

PROTEIN RAN, TESTIS-

PRECURSOR.

[MASS = 88341

PROTEIN HSP 90-BETA.

SPECIFIC ISOFORM.

[MASS = 105719

[MASS = 80701

[MASS = 24451

3
3

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
5

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

PREDICTED: SIMILAR

BETA-2-

RIBOSOMAL PROTEIN S27A.

ATTRACTIN

TO ISOLEUCINE-TRNA

GLYCOPROTEIN 1

[MASS = 17951

PRECURSOR.

SYNTHETASE.

PRECURSOR.

[MASS = 163296

[MASS = 144169

[MASS = 33197

3
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

40S RIBOSOMAL

HISTONE H1.0.

BONE

PREDICTED: NIDOGEN 2.

PROTEIN S3.

[MASS = 20754

MORPHOGENETIC

[MASS = 173960

[MASS = 26674

PROTEIN 1.

[MASS = 111332

3
7

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

PREDICTED similar to

COFILIN-1.

RECEPTOR-LIKE

PREDICTED: SIMILAR TO

HEAT SHOCK PROTEIN

[MASS = 24588

PROTEIN TYROSINE

UBIQUITIN SPECIFIC

86. [MASS = 56953

PHOSPHATASE GAMMA

PROTEASE 9, X-LINKED.

B-TYPE ISOFORM.

[MASS = 290681

[MASS = 156024

3
3

Rattus norvegicus (Rat)
2
4

Rattus norvegicus (Rat)
2
6

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

GAMMA-GLUTAMYL

ALPHA-2-HS-

DERMCIDIN.

SMC4L1 PROTEIN.

HYDROLASE

GLYCOPROTEIN

[MASS = 11284

[MASS = 146806

PRECURSOR.

PRECURSOR.

[MASS = 35830

[MASS = 37982

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

QUIESCIN Q6.

PREDICTED: SIMILAR

CALUMENIN

PREDICTED: similar to

[MASS = 82412

TO LERK-5.

PRECURSOR.

Heterogeneous nuclear

[MASS = 37282

[MASS = 36997

ribonucleoproteins

A2/B1. [MASS = 32468

3
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat) L-
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

LACTATE

PREDICTED: similar to

KINESIN-1 HEAVY CHAIN.

TO DNA REPLICATION

DEHYDROGENASE B

Periostin precursor (PN)

[MASS = 109531

LICENSING FACTOR

CHAIN. [MASS = 36481

(Osteoblast-specific

MCM3. [MASS = 83429

factor 2) (OSF-2).

[MASS = 90252

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
8

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

DELETED IN

PREDICTED:

AMBP PROTEIN

ADAMTS-1 PRECURSOR.

COLORECTAL CANCER.

PROTOCADHERIN 12.

PRECURSOR.

[MASS = 105719

[MASS = 158142

[MASS = 127964

[MASS = 38851

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

ALPHA-ACTININ-1.

PREDICTED: ATPASE,

PROBABLE G-PROTEIN

PREDICTED: SIMILAR TO

[MASS = 102960

H+ TRANSPORTING,

COUPLED RECEPTOR

PLEXIN-B2 PRECURSOR.

LYSOSOMAL

116 PRECURSOR.

[MASS = 216119

ACCESSORY PROTEIN

[MASS = 149446

2. [MASS = 66094

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

COATOMER SUBUNIT

PREDICTED: SIMILAR

PLATELET

PREDICTED: KINESIN FAMILY

BETA′. [MASS = 102420

TO HEAT SHOCK

ENDOTHELIAL CELL

MEMBER 4. [MASS = 139682

PROTEIN HSP 90-BETA.

ADHESION MOLECULE

[MASS = 80701

PRECURSOR.

[MASS = 76189

3
4

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat) NON-

PREDICTED: SIMILAR

IGH-1A PROTEIN.

PREDICTED similar to

ERYTHROCYTE BETA-

TO P59 IMMUNOPHILIN.

[MASS = 51403

FIBRINOGEN, GAMMA

SPECTRIN. [MASS = 251205

[MASS = 80396

POLYPEPTIDE.

[MASS = 49121

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat) D-3-

FIBRINOGEN BETA

FIBRILLIN-2.

PREDICTED:

PHOSPHOGLYCERATE

CHAIN PRECURSOR.

[MASS = 313374

AMINOPEPTIDASE

DEHYDROGENASE.

[MASS = 54303

PUROMYCIN

[MASS = 56362

SENSITIVE.

[MASS = 103344

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

NUCLEOSOME

SPLICE ISOFORM PAM-

SPLICE ISOFORM 1 OF

TO 40S RIBOSOMAL

ASSEMBLY PROTEIN 1-

1 OF PEPTIDYL-

ACETYL-COA CARBOXYLASE

PROTEIN S3.

LIKE 1. [MASS = 45373

GLYCINE ALPHA-

1. [MASS = 265421

[MASS = 26630

AMIDATING

MONOOXYGENASE

PRECURSOR.

[MASS = 108819

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

ALPHA-1-ACID

PREDICTED: SIMILAR

PREDICTED: SIMILAR

NUCLEOLIN. [MASS = 77276

GLYCOPROTEIN

TO UBIQUITIN-

TO FILAMIN A.

PRECURSOR.

CONJUGATING ENZYME

[MASS = 290169

[MASS = 23575

E2 L3. [MASS = 17862

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

ALPHA-1-

FRUCTOSE-

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

MACROGLOBULIN.

BISPHOSPHATE

TO PROTOCADHERIN

SEROTRANSFERRIN

[MASS = 167125

ALDOLASE A.

19 PRECURSOR.

PRECURSOR. [MASS = 76607

[MASS = 39221

[MASS = 125989

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)

LIVER

APOLIPOPROTEIN M

VITAMIN K-DEPENDENT

PREDICTED: HYPOTHETICAL

CARBOXYLESTERASE 1

PRECURSOR.

PROTEIN S

PROTEIN XP_579585.

PRECURSOR.

[MASS = 21513

PRECURSOR.

[MASS = 275729

[MASS = 60175

[MASS = 74627

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

SIMILAR TO RIKEN

HETEROGENEOUS

SEMA4B PROTEIN

PREDICTED: SIMILAR TO

CDNA 2810409H07.

NUCLEAR

(FRAGMENT).

COLONIC AND HEPATIC

[MASS = 44535

RIBONUCLEOPROTEIN

[MASS = 79477

TUMOR OVER-EXPRESSED

C. [MASS = 34385

PROTEIN ISOFORM A.

[MASS = 198456

3
4

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

IG KAPPA CHAIN C

RHO GDP

PREDICTED: SIMILAR TO

TO PHOSPHOLIPID

REGION, B ALLELE.

DISSOCIATION

FILAMIN B. [MASS = 291469

TRANSFER PROTEIN.

[MASS = 11601

INHIBITOR (GDI) ALPHA.

[MASS = 65430

[MASS = 23407

3
3

Rattus norvegicus (Rat)
2
4

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

26S PROTEASE

PYRUVATE KINASE,

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

REGULATORY SUBUNIT

MUSCLE. [MASS = 57976

TO EXPRESSED

CHROMOSOME

8. [MASS = 45626

SEQUENCE C79407.

CONDENSATION PROTEIN G.

[MASS = 113188

[MASS = 113137

3
5

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
13

Rattus norvegicus (Rat) AMBP

PREDICTED: SIMILAR

NUCLEOSIDE

MYOSIN-10.

PROTEIN PRECURSOR.

TO HEAT SHOCK

DIPHOSPHATE KINASE

[MASS = 228965

[MASS = 38851

PROTEIN HSP 90-BETA.

A. [MASS = 17193

[MASS = 54660

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

SUPEROXIDE

TUBULIN, BETA, 2.

GM2 GANGLIOSIDE

Neogenin precursor.

DISMUTASE.

[MASS = 49801

ACTIVATOR PROTEIN.

[MASS = 156144

[MASS = 15780

[MASS = 21493

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

PREDICTED:

PREDICTED:

PREDICTED:

LEUCYL-TRNA SYNTHETASE.

HYPOTHETICAL

GLYCOPROTEIN-4-

PROCOLLAGEN, TYPE

[MASS = 134279

PROTEIN XP_579585.

BETA-

XII, ALPHA 1.

[MASS = 275729

GALACTOSYLTRANSFERASE

[MASS = 367709

2. [MASS = 44484

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

LOW-DENSITY

ALPHA-1-ACID

EPSILON 2 GLOBIN.

PREDICTED:

LIPOPROTEIN

GLYCOPROTEIN

[MASS = 16388

THROMBOSPONDIN 4.

RECEPTOR

PRECURSOR.

[MASS = 121361

PRECURSOR.

[MASS = 23575

[MASS = 96622

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
4

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

BETA-1,3-N-

HISTONE H1.2.

PREDICTED: SIMILAR TO

TO PROTOCADHERIN 18

ACETYLGLUCOSAMINYL-

[MASS = 21856

TALIN 2. [MASS = 273281

PRECURSOR.

TRANSFERASE

LUNATIC FRINGE.

[MASS = 123552

[MASS = 41958

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

SPLICE ISOFORM B OF

PREDICTED: SIMILAR

60S RIBOSOMAL

PREDICTED: SIMILAR TO

AP-1 COMPLEX

TO ELASTIN

PROTEIN L8.

OLLISTATIN-LIKE 5.

SUBUNIT BETA-1.

MICROFIBRIL

[MASS = 27893

[MASS = 95918

[MASS = 103873

INTERFACER 1.

[MASS = 107560

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

COLLAGEN ALPHA-2(I)

PREDICTED: SIMILAR

PREDICTED: SIMILAR

THROMBOSPONDIN 1.

CHAIN PRECURSOR.

TO HETEROGENEOUS

TO LIPOPROTEIN

[MASS = 129671

[MASS = 129564

NUCLEAR

RECEPTOR-RELATED

RIBONUCLEOPROTEIN

PROTEIN.

A2/B1. [MASS = 38284

[MASS = 504889

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)

SHEN-DAN.

TPA: proteasome subunit

PREDICTED: SIMILAR

STATHMIN. [MASS = 17157

[MASS = 131080

beta type 6-like.

TO SUSHI, VON

[MASS = 25304

WILLEBRAND FACTOR

TYPE A, EGF AND

PENTRAXIN DOMAIN

CONTAINING 1.

[MASS = 383558

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)

SPARC-LIKE PROTEIN 1

BETA-2-

GTP-BINDING NUCLEAR

PREDICTED:

PRECURSOR.

GLYCOPROTEIN 1

PROTEIN RAN, TESTIS-

GLYCOPROTEIN-4-BETA-

[MASS = 70634

PRECURSOR.

SPECIFIC ISOFORM.

GALACTOSYLTRANSFERASE

[MASS = 33197

[MASS = 24451

2. [MASS = 44484

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
4

Rattus norvegicus (Rat)

NUCLEOSIDE

GRP78 BINDING

14-3-3 PROTEIN

HISTONE H1.2. [MASS = 21856

DIPHOSPHATE KINASE

PROTEIN.

EPSILON. [MASS = 29174

B. [MASS = 17283

[MASS = 110574

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)
3
3

Rattus norvegicus (Rat)

D-3-

HAPTOGLOBIN

INSULIN-LIKE GROWTH

PEROXIREDOXIN-1.

PHOSPHOGLYCERATE

PRECURSOR.

FACTOR-BINDING

[MASS = 22109

DEHYDROGENASE.

[MASS = 38549

PROTEIN COMPLEX

[MASS = 56362

ACID LABILE CHAIN

PRECURSOR.

[MASS = 66812

3
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat)

PROTEIN DISULFIDE-

PREDICTED: similar to

ALPHA-ENOLASE.

ISOMERASE A3

alpha 1 type II collagen.

[MASS = 46997

PRECURSOR.

[MASS = 138706

[MASS = 57079

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
4

Rattus norvegicus (Rat) RAB

LARGE PROLINE-RICH

COLLAGEN TYPE

GDP DISSOCIATION

PROTEIN BAT3.

A1(XI)7-8. [MASS = 45691

INHIBITOR ALPHA.

[MASS = 114647

[MASS = 50537

3
5

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PROPROTEIN

ANGIOTENSIN-CONVERTING

TO IMPORTIN 9.

CONVERTASE

ENZYME, SOMATIC ISOFORM

[MASS = 131739

SUBTILISIN/KEXIN TYPE

PRECURSOR. [MASS = 150908

9 PRECURSOR.

[MASS = 74709

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

NUCLEIC ACID BINDING

PREDICTED: SIMILAR TO

TO 25 KDA FK506-

FACTOR PRM10.

ALPHA ENOLASE.

BINDING PROTEIN.

[MASS = 33815

[MASS = 46489

[MASS = 25179

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
4

Rattus norvegicus (Rat)

DNA POLYMERASE

PREDICTED: SIMILAR

PREDICTED similar to HEAT

DELTA CATALYTIC

TO ALPHA 3 TYPE VI

SHOCK PROTEIN 86.

SUBUNIT.

COLLAGEN ISOFORM 1

[MASS = 56953

[MASS = 123601

PRECURSOR.

[MASS = 369017

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) ALPHA

CHAPERONIN SUBUNIT

MANNOSIDASE 2,

ACTININ 4. [MASS = 104915

6A. [MASS = 58017

ALPHA B1.

[MASS = 114327

3
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

SPLICE ISOFORM 2 OF

METLLOPROTEINASE

SPLICE ISOFORM APP770 OF

INTERLEUKIN

INHIBITOR 1

AMYLOID BETA A4 PROTEIN

ENHANCER-BINDING

PRECURSOR.

PRECURSOR (FRAGMENT).

FACTOR 3.

[MASS = 23794

[MASS = 86704

[MASS = 97680

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

CHAPERONIN

PREDICTED similar to T-

REELIN PRECURSOR.

CONTAINING TCP1,

KININOGEN 2 PRECURSOR

[MASS = 387531

SUBUNIT 5.

(Fragment). [MASS = 72419

[MASS = 59537

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

TO DNA REPLICATION

TO VESICULAR

HEAT SHOCK 70 KDA

LICENSING FACTOR

INTEGRAL-MEMBRANE

PROTEIN 4 LIKE.

MCM2. [MASS = 102272

PROTEIN VIP36

[MASS = 136266

PRECURSOR.

[MASS = 40393

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
4

Rattus norvegicus (Rat)

40S RIBOSOMAL

IRON-RESPONSIVE

TUBULIN BETA-3 CHAIN.

PROTEIN S18.

ELEMENT-BINDING

[MASS = 50419

[MASS = 17719

PROTEIN 1.

[MASS = 98128

3
4

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

HISTIDINE-RICH

RIBONUCLEOTIDE

EUKARYOTIC TRANSLATION

GLYCOPROTEIN.

REDUCTASE M1.

INITIATION FACTOR 5A-1.

[MASS = 57581

[MASS = 90293

[MASS = 16701

3
4

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
4

Rattus norvegicus (Rat)

HISTONE H1.2.

60S ACIDIC RIBOSOMAL

PYRUVATE KINASE,

[MASS = 21856

PROTEIN P0.

MUSCLE. [MASS = 57976

[MASS = 34215

3
3

Rattus norvegicus (Rat)
2
4

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

DYNACTIN-1.

AMBP PROTEIN

PREDICTED: SIMILAR TO

[MASS = 141930

PRECURSOR.

PEPTIDOGLYCAN

[MASS = 38851

RECOGNITION PROTEIN 2.

[MASS = 39775

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) L-

PREDICTED: SIMILAR

SYNTENIN-1.

LACTATE DEHYDROGENASE

TO REGULATOR OF

[MASS = 32423

B CHAIN. [MASS = 36481

NONSENSE

TRANSCRIPTS 1.

[MASS = 88226

3
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) IGH-1A

VIGILIN. [MASS = 141584

RECEPTOR-LIKE

PROTEIN. [MASS = 51403

PROTEIN TYROSINE

PHOSPHATASE KAPPA

EXTRACELLULAR

REGION. [MASS = 56159

3
4

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
4

Rattus norvegicus (Rat)

60S RIBOSOMAL

PREDICTED: SIMILAR

ALPHA-2-HS-GLYCOPROTEIN

PROTEIN L3.

TO HEMICENTIN 1.

PRECURSOR. [MASS = 37982

[MASS = 46005

[MASS = 639647

3
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

ALPHA-MANNOSIDASE

EPITHELIAL-CADHERIN

SPLICE ISOFORM IIBA OF

2. [MASS = 131242

PRECURSOR.

DYNAMIN-2. [MASS = 98246

[MASS = 98715

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PROTOCADHERIN.

VASCULAR CELL

SORTILIN PRECURSOR.

[MASS = 505997

ADHESION PROTEIN 1

[MASS = 91169

PRECURSOR.

[MASS = 81246

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat)

PROLIFERATION-

PREDICTED: SIMILAR

HEMOGLOBIN BETA-1

ASSOCIATED 2G4,

TO CG1841-PA,

SUBUNIT. [MASS = 15848

38 KDA. [MASS = 43657

ISOFORM A.

[MASS = 52522

3
5

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

STATHMIN.

CLUSTERIN

TRANSLATIONALLY-

[MASS = 17157

PRECURSOR.

CONTROLLED TUMOR

[MASS = 51375

PROTEIN. [MASS = 19462

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
4

Rattus norvegicus (Rat) ALPHA

PREDICTED: similar to

PREDICTED: SIMILAR

2 MACROGLOBULIN

60S ribosomal protein

TO 40S RIBOSOMAL

CARDIAC ISOFORM.

L38. [MASS = 8215

PROTEIN S3.

[MASS = 163218

[MASS = 26630

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) RAT

40S RIBOSOMAL

PREDICTED to similar to

ALPHA(1)-INHIBITOR 3,

PROTEIN S7.

HEAT SHOCK PROTEIN

VARIANT I PRECURSOR.

[MASS = 22127

HSP 90-BETA.

[MASS = 165326

[MASS = 50669

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

COLLAGEN ALPHA-1(V)

60S RIBOSOMAL

PROTEASOME SUBUNIT

CHAIN PRECURSOR.

PROTEIN L13.

BETA TYPE 1. [MASS = 26479

[MASS = 184610

[MASS = 24178

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

HAUSP. [MASS = 128431

ARCADLIN.

PREDICTED: SIMILAR TO

[MASS = 103800

IMMUNOGLOBULIN HEAVY

CHAIN. [MASS = 120447

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PROTOCADHERIN

PREDICTED: SIMILAR

SPLICE ISOFORM B OF AP-1

GAMMA SUBFAMILY C,

TO CELLULAR

COMPLEX SUBUNIT BETA-1.

3. [MASS = 101038

APOPTOSIS

[MASS = 103873

SUSCEPTIBILITY

PROTEIN.

[MASS = 110214

3
5

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat) GPI-

NUCLEOLIN.

PREDICTED: SIMILAR

ANCHORED MEMBRANE

[MASS = 77276

TO SEMAPHORIN 6D-4.

PROTEIN 1. [MASS = 75707

[MASS = 159473

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) T-

PREDICTED: SIMILAR

60S RIBOSOMAL

COMPLEX PROTEIN 1

TO UBIQUITIN SPECIFIC

PROTEIN L3.

SUBUNIT DELTA.

PROTEASE 9, X-LINKED.

[MASS = 46005

[MASS = 57968

[MASS = 290681

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TENASCIN

PROTECTIVE PROTEIN FOR

TO ENHANCER-TRAP-

(FRAGMENT).

BETA-GALACTOSIDASE.

LOCUS-1.

[MASS = 67815

[MASS = 51216

[MASS = 159154

3
6

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PHOSPHOGLYCERATE

HEAT SHOCK 70 KDA

PREDICTED similar to

MUTASE 2.

PROTEIN 1A/1B.

Proteasome 26S subunit,

[MASS = 28624

[MASS = 70185

ATPase 3. [MASS = 50509

3
5

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

DAMAGE-SPECIFIC DNA

PREDICTED: similar to

PREDICTED: ADAPTOR-

BINDING PROTEIN 1.

Slit-like 2. [MASS = 72321

RELATED PROTEIN

[MASS = 127059

COMPLEX 1, GAMMA 1

SUBUNIT. [MASS = 91693

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

TO SIDEKICK 2.

TO POLY(RC)-BINDING

HSPC263. [MASS = 37041

[MASS = 466498

PROTEIN 1.

[MASS = 37498

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat)

PEPTIDYLPROLYL

C-REACTIVE PROTEIN

PREDICTED: SIMILAR TO

ISOMERASE C.

PRECURSOR.

ALPHA NAC/1.9.2. PROTEIN.

[MASS = 23009

[MASS = 25468

[MASS = 23384

3
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat)

TENASCIN

PREDICTED similar to

ALPHA-ACTININ-4.

(FRAGMENT).

FIBRINOGEN, GAMMA

[MASS = 104786

[MASS = 67815

POLYPEPTIDE.

[MASS = 49121

3
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

NEUROSERPIN

PREDICTED: VON

TO RIBOSOMAL

PRECURSOR.

WILLEBRAND FACTOR.

PROTEIN L28.

[MASS = 46278

[MASS = 308474

[MASS = 16313

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) FATTY

ALPHA-ENOLASE.

PREDICTED: SIMILAR

ACID-BINDING PROTEIN,

[MASS = 46997

TO PUTATIVE PRE-

BRAIN. [MASS = 14733

MRNA SPLICING

FACTOR RNA

HELICASE.

[MASS = 90977

2
2

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) GM2

PREDICTED similar to T-

SERINE (OR CYSTEINE

GANGLIOSIDE ACTIVATOR

KININOGEN 2

PROTEINASE

PROTEIN. [MASS = 21493

PRECURSOR

INHIBITOR, CLADE A

(Fragment)

(ALPHA-1

[MASS = 72419

ANTIPROTEINASE,

ANTITRYPSIN),

MEMBER 6.

[MASS = 44671

2
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) SSB

HYRAC. [MASS = 31353

HEMOPEXIN

PROTEIN. [MASS = 43926

PRECURSOR.

[MASS = 51291

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

HEAT SHOCK-RELATED

PREDICTED: BRAIN

MANNOSIDASE, ALPHA,

70 KDA PROTEIN 2.

GLYCOGEN

CLASS 1A, MEMBER 1.

[MASS = 69528

PHOSPHORYLASE.

[MASS = 73125

[MASS = 96738

2
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PROLIFERATING CELL

PREDICTED: SIMILAR

VIGILIN. [MASS = 141584

NUCLEAR ANTIGEN.

TO 40S RIBOSOMAL

[MASS = 28749

PROTEIN S9.

[MASS = 22648

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR

PREDICTED-MATRIN-3.

TO ALPHA ENOLASE.

TO 60S RIBOSOMAL

[MASS = 44733

[MASS = 27286

PROTEIN L12.

[MASS = 17847

2
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

HYDROXYMETHYL-

SEZ6B. [MASS = 105607

SPLICE ISOFORM 1 OF

GLUTARYL-

FIBRINOGEN ALPHA CHAIN

COA SYNTHASE,

PRECURSOR. [MASS = 86686

CYTOPLASMIC.

[MASS = 57434

2
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

SEMA4B PROTEIN

EPITHELIAL-CADHERIN

TO HEAT SHOCK 70 KDA

(FRAGMENT).

PRECURSOR. [MASS = 98715

PROTEIN 4 LIKE.

[MASS = 79477

[MASS = 136266

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

RUVB-LIKE 2.

SPLICE ISOFORM 1 OF

FRUCTOSE-BISPHOSPHATE

[MASS = 51147

NEUROFASCIN

ALDOLASE C. [MASS = 39153

PRECURSOR.

[MASS = 138004

2
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

SPLICE ISOFORM 2 OF

PREDICTED: SIMILAR

TUBULIN BETA CHAIN.

PLASMINOGEN

TO COLLAGEN ALPHA1

[MASS = 49963

ACTIVATOR INHIBITOR

TYPE VI-PRECURSOR.

1 RNA-BINDING

[MASS = 130760

PROTEIN. [MASS = 42984

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

RUVB-LIKE 1.

60S ACIDIC RIBOSOMAL

ISOCITRATE

[MASS = 50214

PROTEIN P2.

DEHYDROGENASE [NADP]

[MASS = 11692

CYTOPLASMIC. [MASS = 46734

2
2

Rattus norvegicus (Rat)
2
5

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

GLUTATHIONE S-

DERMCIDIN.

PREDICTED: SIMILAR TO

TRANSFERASE P.

[MASS = 11284

EUKARYOTIC TRANSLATION

[MASS = 23308

INITIATION FACTOR 4,

GAMMA 1 ISOFORM A.

[MASS = 93472

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) HEAT

MANNOSIDASE, ALPHA,

EPSILON 2 GLOBIN.

SHOCK-RELATED 70 KDA

CLASS 1A, MEMBER 1.

[MASS = 16388

PROTEIN 2. [MASS = 69528

[MASS = 73125

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

RHO GDP

TUBULIN BETA CHAIN.

KINESIN-LIKE PROTEIN

DISSOCIATION

[MASS = 49963

KIF15. [MASS = 159554

INHIBITOR (GDI) ALPHA.

[MASS = 23407

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

SPLICE ISOFORM

PREDICTED: SIMILAR

DAMAGE-SPECIFIC DNA

PYBP1 OF

TO SERINE PROTEASE

BINDING PROTEIN 1.

POLYPYRIMIDINE

INHIBITOR 2.4.

[MASS = 127059

TRACT-BINDING

[MASS = 46841

PROTEIN 1.

[MASS = 56937

2
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) HEAT

PREDICTED-HEAT

PREDICTED: SIMILAR

SHOCK 70 KDA PROTEIN

SHOCK PROTEIN HSP

TO COATOMER

1A/1B. [MASS = 70185

90-BETA (Frgament).

PROTEIN COMPLEX

[MASS = 50669

SUBUNIT ALPHA.

[MASS = 138360

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

METALLOPROTEINASE

PREDICTED: similar to

PREDICTED: SIMILAR TO

INHIBITOR 1

Periostin precursor (PN)

GLYCERALDEHYDE-3-

PRECURSOR.

(Osteoblast-specific

PHOSPHATE

[MASS = 23794

factor 2) (OSF-2).

DEHYDROGENASE.

[MASS = 90252

[MASS = 35200

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat)

PREDICTED: NIDOGEN

SPLICE ISOFORM 1 OF

COLLAGEN ALPHA-2(I)

2. [MASS = 173960

MYOSIN-11

CHAIN PRECURSOR.

[FRAGMENT).

[MASS = 129564

[MASS = 152492

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

40S RIBOSOMAL

EPSILON 3 GLOBIN.

TO ENO1 PROTEIN.

PROTEIN S10.

[MASS = 16540

[MASS = 47532

[MASS = 18916

2
2

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

SORTILIN PRECURSOR.

HISTIDINE-RICH

HEMOPEXIN PRECURSOR.

[MASS = 91169

GLYCOPROTEIN.

[MASS = 51291

[MASS = 57581

2
3

Rattus norvegicus (Rat)
2
3

Rattus norvegicus (Rat)

2
6

Rattus norvegicus (Rat)

60S ACIDIC RIBOSOMAL

RAB GDP

COLLAGEN ALPHA-1(V)

PROTEIN P2.

DISSOCIATION

CHAIN PRECURSOR.

[MASS = 11692

INHIBITOR BETA.

[MASS = 184610

[MASS = 50685

2
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) 1-

FOLLISTATIN-RELATED

LIVER

PHOSPHATIDYLINOSITOL-

PROTEIN 1

CARBOXYLESTERASE 1

4,5-BISPHOSPHATE

PRECURSOR.

PRECURSOR.

PHOSPHODIESTERASE

[MASS = 34622

[MASS = 60175

GAMMA 1. [MASS = 148548

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
5

Rattus norvegicus (Rat) BETA-

PREDICTED: SIMILAR

PREDICTED: TUMOR

2-MICROGLOBULIN

TO PSMC6 PROTEIN.

REJECTION ANTIGEN

PRECURSOR. [MASS = 13720

[MASS = 45797

GP96. [MASS = 92771

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat)

SYNTENIN-1.

LOC362795 PROTEIN.

STRUCTURAL

[MASS = 32423

[MASS = 52392

MAINTENANCE OF

CHROMOSOME 1-LIKE 1

PROTEIN. [MASS = 143205

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat) T-

INOSINE

PREDICTED: SIMILAR

CADHERIN. [MASS = 78086

MONOPHOSPHATE

TO UBIQUITIN

DEHYDROGENASE 2.

CARBOXYL-TERMINAL

[MASS = 55799

HYDROLASE 5.

[MASS = 95779

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) BETA-

PREDICTED: ATPASE,

INOSINE

2-GLYCOPROTEIN 1

H+ TRANSPORTING,

MONOPHOSPHATE

PRECURSOR. [MASS = 33197

LYSOSOMAL

DEHYDROGENASE 2.

ACCESSORY PROTEIN

[MASS = 55799

2. [MASS = 66094

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

DNA PRIMASE LARGE

HYPOTHETICAL

CALCIUM-DEPENDENT

SUBUNIT. [MASS = 58603

PROTEIN RGD1305890.

SECRETION ACTIVATOR 1.

[MASS = 31776

[MASS = 146266

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

EUKARYOTIC

PREDICTED: SIMILAR

EUKARYOTIC TRANSLATION

TRANSLATION

TO BETA-

INITIATION FACTOR 3

INITIATION FACTOR 3

GALACTOSIDASE

SUBUNIT 9. [MASS = 107985

SUBUNIT 9.

PRECURSOR.

[MASS = 107985

[MASS = 73228

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

MICROFIBRILLAR-

ADAPTOR PROTEIN

SEX HORMONE-

ASSOCIATED PROTEIN

COMPLEX AP-2, ALPHA 2

BINDING GLOBULIN

4. [MASS = 29050

SUBUNIT. [MASS = 104174

PRECURSOR.

[MASS = 44533

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
4

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR

NUCLEOSOME ASSEMBLY

TO COLLAGEN ALPHA1

TO LAMININ ALPHA-1

PROTEIN 1-LIKE 1.

TYPE VI-PRECURSOR.

CHAIN PRECURSOR-

[MASS = 45373

[MASS = 130760

MOUSE. [MASS = 338692

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

SPLICEOSOME RNA

PROTHROMBIN PRECURSOR

TO 26S PROTEASOME

HELICASE BAT1.

(FRAGMENT). [MASS = 70412

NON-ATPASE

[MASS = 49035

REGULATORY SUBUNIT

11. [MASS = 54019

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED-

60S RIBOSOMAL

PREDICTED: CADHERIN 11.

HETEROGENEOUS

PROTEIN L24.

[MASS = 88036

NUCLEAR

[MASS = 17779

RIBONUCLEOPROTEIN

A1. [MASS = 33656

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) M-

GUANINE NUCLEOTIDE-

PREDICTED: SIMILAR

CADHERIN. [MASS = 85753

BINDING PROTEIN BETA

TO STABILIN-1.

SUBUNIT 2-LIKE 1.

[MASS = 288663

[MASS = 35419

2
3

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED:

PREDICTED: SIMILAR

PREDICTED: similar to

EUKARYOTIC

TO 26S PROTEASOME

ubiquitin-activating enzyme E1.

TRANSLATION

NON-ATPASE

[MASS = 117931

ELONGATION FACTOR 1

REGULATORY SUBUNIT

GAMMA. [MASS = 72445

11. [MASS = 54019

2
2

Rattus norvegicus (Rat)
2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: similar to

SPLICEOSOME RNA

TO DEAD/H BOX

60S ribosomal protein

HELICASE BAT1.

POLYPEPTIDE 36

L29. [MASS = 16961

[MASS = 49035

PROTEIN.

[MASS = 113843

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

TO PYRUVATE KINASE

RANBP4. [MASS = 118926

3. [MASS = 84928

2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: PROCOLLAGEN,

TO HSPC263.

TYPE XII, ALPHA 1.

[MASS = 37041

[MASS = 367709

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

FIBRILLIN-2.

PREDICTED: SIMILAR TO

[MASS = 313374

SLIT-ROBO RHO GTPASE-

ACTIVATING PROTEIN 1.

[MASS = 133329

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

15 KDA PROTEIN.

PREDICTED: HISTONE

[MASS = 14671

DEACETYLASE 6.

[MASS = 168631

2
2

Rattus norvegicus (Rat)

2
5

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

DERMCIDIN. [MASS = 11284

FIBRINOGEN ALPHA

CHAIN PRECURSOR.

[MASS = 86686

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

40S RIBOSOMAL

TRIOSEPHOSPHATE

PROTEIN S13.

ISOMERASE. [MASS = 26790

[MASS = 17091

2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

GLUCOSAMINE.

DNA-BINDING PROTEIN

[MASS = 60914

A. [MASS = 38852

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

RETINOL-BINDING

PREDICTED: SIMILAR TO N-

PROTEIN I, CELLULAR.

TERMINAL

[MASS = 15703

ACEYLTRANSFERASE 1.

[MASS = 100994

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

TPA: proteasome subunit

SPLICE ISOFORM 2 OF DNA.

beta type 6-like.

[MASS = 173853

[MASS = 25304

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PROTEIN ARGININE N-

PROTEASOME SUBUNIT

METHYLTRANSFERASE

BETA TYPE 2. [MASS = 22912

1. [MASS = 42436

2
2

Rattus norvegicus (Rat)

2
4

Rattus norvegicus (Rat) 14-3-3

TRANSLATIONALLY-

PROTEIN THETA.

CONTROLLED TUMOR

[MASS = 27778

PROTEIN. [MASS = 19462

2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) 14-3-3

PREDICTED: SIMILAR

PROTEIN BETA/ALPHA.

TO STABILIN-1.

[MASS = 27923

[MASS = 288663

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

60S RIBOSOMAL

CALMODULIN. [MASS = 16706

PROTEIN L7.

[MASS = 30329

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

INSULIN-LIKE GROWTH

TO SERINE PROTEASE

FACTOR-BINDING PROTEIN

INHIBITOR 2.4.

COMPLEX ACID LABILE

[MASS = 46841

CHAIN PRECURSOR.

[MASS = 66812

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

NUCLEOSIDE

PREDICTED:

DIPHOSPHATE KINASE

CHROMODOMAIN HELICASE

A. [MASS = 17193

DNA BINDING PROTEIN 4.

[MASS = 222452

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

EUKARYOTIC

PREDICTED: SIMILAR TO

TRANSLATION

RIBOSOMAL PROTEIN L6.

INITIATION FACTOR 5A-

[MASS = 32944

1. [MASS = 16701

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED-40S

PREDICTED: SIMILAR TO

ribosomal protein S17.

STABILIN-1. [MASS = 288663

[MASS = 16340

2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

SPLICE ISOFORM 2 OF

TENASCIN (FRAGMENT).

RECEPTOR-TYPE

[MASS = 67815

TYROSINE-PROTEIN

PHOSPHATASE ZETA

PRECURSOR.

[MASS = 164596

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

TO CCR4-NOT

LIPOPROTEIN RECEPTOR-

TRANSCRIPTION

RELATED PROTEIN.

COMPLEX, SUBUNIT 1

[MASS = 504889

ISOFORM A.

[MASS = 93683

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) ZERO

HEAT SHOCK 70 KDA

BETA-1 GLOBIN.

PROTEIN 1A/1B.

[MASS = 16023

[MASS = 70185

2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) GTP-

RECEPTOR-LIKE

BINDING NUCLEAR PROTEIN

PROTEIN TYROSINE

RAN, TESTIS-SPECIFIC

PHOSPHATASE KAPPA

ISOFORM. [MASS = 24451

EXTRACELLULAR

REGION. [MASS = 56159

2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

NUCLEIC ACID BINDING

COLLAGEN ALPHA-1(III)

FACTOR PRM10.

CHAIN PRECURSOR.

[MASS = 33815

[MASS = 138936

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

SPLICE ISOFORM

PREDICTED: SIMILAR TO

APP770 OF AMYLOID

HEPATIC MULTIPLE

BETA A4 PROTEIN

INOSITOL POLYPHOSPHATE

PRECURSOR

PHOSPHATASE.

(FRAGMENT).

[MASS = 54619

[MASS = 86704

2
5

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: similar to

PREDICTED: SIMILAR TO

Fibulin-1 precursor.

MUCIN 17. [MASS = 189893

[MASS = 75381

2
2

Rattus norvegicus (Rat)

2
9

Rattus norvegicus (Rat)

CADHERIN EGF LAG

SERINE (OR CYSTEINE)

SEVEN-PASS G-TYPE

PROTEINASE INHIBITOR,

RECEPTOR 2.

CLADE A (ALPHA-1

[MASS = 317122

ANTIPROTEINASE,

ANTITRYPSIN), MEMBER 6.

[MASS = 44671

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

FARNESYL

TO ARX. [MASS = 121446

PYROPHOSPHATE

SYNTHETASE. [MASS = 40830

2
4

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat)

HETEROGENEOUS

PREDICTED: SIMILAR TO

NUCLEAR

HEPARAN SULFATE

RIBONUCLEOPROTEIN

PROTEOGLYCAN 2.

C. [MASS = 34385

[MASS = 377284

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED:

PREDICTED: SIMILAR TO

PROTEASOME

ELAV. [MASS = 49528

(PROSOME,

MACROPAIN) SUBUNIT,

BETA TYPE 5.

[MASS = 37128

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

109 KDA PROTEIN.

PREDICTED: SIMILAR TO

[MASS = 108509

TUBULIN-SPECIFIC

CHAPERONE D.

[MASS = 60160

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

AMBP PROTEIN

COATOMER SUBUNIT BETA′.

PRECURSOR.

[MASS = 102420

[MASS = 38851

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PROTEASOME.

PREDICTED: SIMILAR TO

[MASS = 60688

EXPRESSED SEQUENCE

AI314180. [MASS = 203921

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

TO RIBOSOMAL

REGULATOR OF NONSENSE

PROTEIN L14.

TRANSCRIPTS 1.

[MASS = 18408

[MASS = 88226

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

LOW-DENSITY

PREDICTED: SIMILAR TO

LIPOPROTEIN

SORCSB SPLICE VARIANT

RECEPTOR-RELATED

OF THE VPS10 DOMAIN

PROTEIN 2

RECEPTOR SORCS.

PRECURSOR.

[MASS = 129969

[MASS = 519276

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

GRP78 BINDING

PREDICTED: SIMILAR TO 40S

PROTEIN.

RIBOSOMAL PROTEIN S19.

[MASS = 110574

[MASS = 16172

2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

TO COLLAGEN ALPHA

CYFIP1 PROTEIN.

2(IV) CHAIN

[MASS = 144933

PRECURSOR - MOUSE.

[MASS = 192535

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

60S RIBOSOMAL

PREDICTED similar to

PROTEIN L7A.

FIBRINOGEN, GAMMA

[MASS = 29864

POLYPEPTIDE. [MASS = 49121

2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED-Proteasome

PREDICTED: SIMILAR TO

26S subunit, ATPase 3.

LAMININ B1. [MASS = 228429

[MASS = 50509

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

URIDINE MONOPHOSPHATE

TO N-TERMINAL

SYNTHETASE. [MASS = 52379

ACEYLTRANSFERASE 1.

[MASS = 100994

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

O-GLCNACASE.

PREDICTED: SIMILAR TO

[MASS = 102918

PROTOCADHERIN 18

PRECURSOR. [MASS = 123552

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

40S RIBOSOMAL

ASCC3L1 PROTEIN.

PROTEIN S21.

[MASS = 244875

[MASS = 9127

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PROLIFERATION-

TO CYFIP1 PROTEIN.

ASSOCIATED 2G4, 38 KDA.

[MASS = 144933

[MASS = 43657

2
4

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) T-

SPLICE ISOFORM 1 OF

COMPLEX PROTEIN 1

40S RIBOSOMAL

SUBUNIT ALPHA.

PROTEIN S24.

[MASS = 60360

[MASS = 15423

2
7

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

DERMCIDIN.

INOSINE MONOPHOSPHATE

[MASS = 11284

DEHYDROGENASE 2.

[MASS = 55799

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) 60S

PREDICTED: SIMILAR

RIBOSOMAL PROTEIN L7A.

TO 60S RIBOSOMAL

[MASS = 29864

PROTEIN L26.

[MASS = 24366

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) LOW-

PREDICTED: SPLICING

DENSITY LIPOPROTEIN

FACTOR 3B, SUBUNIT 1.

RECEPTOR PRECURSOR.

[MASS = 152445

[MASS = 96622

2
2

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat)

HAPTOGLOBIN

PREDICTED: SIMILAR TO

PRECURSOR.

NISCHARIN. [MASS = 148481

[MASS = 38549

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

TO EXPRESSED

GTPASE ACTIVATING

SEQUENCE AI314180.

PROTEIN AND VPS9

[MASS = 203921

DOMAINS 1. [MASS = 160359

2
2

Rattus norvegicus (Rat)

2
4

Rattus norvegicus (Rat)

ARCADLIN.

PREDICTED: SIMILAR TO

[MASS = 103800

ALDEHYDE

DEHYDROGENASE FAMILY 7,

MEMBER A1. [MASS = 58749

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

EPITHELIAL-CADHERIN

NUCLEOSIDE DIPHOSPHATE

PRECURSOR.

KINASE B. [MASS = 17283

[MASS = 98715

2
3

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

COMPLEMENT COMPONENT

TO 60S RIBOSOMAL

C6 PRECURSOR.

PROTEIN L7A.

[MASS = 105114

[MASS = 13842

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: TYROSINE

TO NISCHARIN.

KINASE RECEPTOR 1.

[MASS = 148481

[MASS = 125210

2
2

Rattus norvegicus (Rat)

2
4

Rattus norvegicus (Rat)

SPARC PRECURSOR.

HEMOGLOBIN ALPHA-1/2

[MASS = 34384

SUBUNIT. [MASS = 15197

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) 40S

SERINE (OR CYSTEINE)

RIBOSOMAL PROTEIN S18.

PROTEINASE

[MASS = 17719

INHIBITOR, CLADE A

(ALPHA-1

ANTIPROTEINASE,

ANTITRYPSIN), MEMBER

6. [MASS = 44671

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

VESICLE ASSOCIATED

SPLICE ISOFORM V0 OF

PROTEIN.

VERSICAN CORE PROTEIN

[MASS = 135350

PRECURSOR (FRAGMENT).

[MASS = 300008

2
3

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat)

HYPOTHETICAL

PREDICTED: SIMILAR TO

PROTEIN ALDOAL1.

MAM DOMAIN CONTAINING

[MASS = 39492

2. [MASS = 68019

2
2

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

PREDICTED: SIMILAR TO

TO EUKARYOTIC

PUTATIVE E3 LIGASE.

TRANSLATION

[MASS = 526904

INITIATION FACTOR 4,

GAMMA 1 ISOFORM A.

[MASS = 93472

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

SPLICE ISOFORM CDK2-

INSULIN-LIKE GROWTH

ALPHA OF CELL

FACTOR 1 RECEPTOR

DIVISION PROTEIN

PRECURSOR. [MASS = 155524

KINASE 2. [MASS = 33887

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

COMPLEMENT

GLUCOSIDASE, ALPHA;

COMPONENT 2.

ACID. [MASS = 106207

[MASS = 83699

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

ZINC

COMPLEMENT COMPONENT

PHOSPHODIESTERASE

1, S SUBCOMPONENT.

ELAC PROTEIN 2.

[MASS = 77713

[MASS = 92340

2
2

Rattus norvegicus (Rat)

2
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

SPLICE ISOFORM 2 OF

TO 116 KDA U5 SMALL

TROPOMYOSIN BETA CHAIN.

NUCLEAR

[MASS = 32958

RIBONUCLEOPROTEIN

COMPONENT.

[MASS = 109478

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

HYPOTHETICAL PROTEIN

TO 40S RIBOSOMAL

ALDOAL1. [MASS = 39492

PROTEIN S16.

[MASS = 20192

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) DNA

SERINE/THREONINE-

POLYMERASE DELTA

PROTEIN

CATALYTIC SUBUNIT.

PHOSPHATASE 2A

[MASS = 123601

CATALYTIC SUBUNIT

BETA ISOFORM.

[MASS = 35575

2
2

Rattus norvegicus (Rat)

2
4

Rattus norvegicus (Rat) AC2-

MATRIN-3. [MASS = 94447

008. [MASS = 26204

2
2

Rattus norvegicus (Rat)

2
2

Rattus norvegicus (Rat) PRX

LEUKEMIA INHIBITORY

IV. [MASS = 31007

FACTOR RECEPTOR

PRECURSOR.

[MASS = 122394

2
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO POLY(RC)-BINDING

PROTEIN 1.

[MASS = 37498

2
2

Rattus norvegicus (Rat)

LRRGT00164.

[MASS = 111258

2
2

Rattus norvegicus (Rat)

DNA LIGASE 1.

[MASS = 102482

2
2

Rattus norvegicus (Rat)

HEMOGLOBIN ALPHA-

1/2 SUBUNIT.

[MASS = 15197

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO RIBOSOMAL

PROTEIN L34.

[MASS = 13582

2
2

Rattus norvegicus (Rat)

TRANSKETOLASE.

[MASS = 71159

2
2

Rattus norvegicus (Rat)

SPLICE ISOFORM 1 OF

HETEROGENEOUS

NUCLEAR

RIBONUCLEOPROTEIN

M. [MASS = 74350

2
3

Rattus norvegicus (Rat)

HISTONE H2A.

[MASS = 14189

2
2

Rattus norvegicus (Rat)

40S RIBOSOMAL

PROTEIN S25.

[MASS = 13742

2
2

Rattus norvegicus (Rat)

HYPOTHETICAL

PROTEIN RGD1305890.

[MASS = 31776

2
2

Rattus norvegicus (Rat)

SSB PROTEIN.

[MASS = 43926

2
2

Rattus norvegicus (Rat)

C-REACTIVE PROTEIN

PRECURSOR.

[MASS = 25468

2
2

Rattus norvegicus (Rat)

GLUCOSAMINE.

[MASS = 60914

2
2

Rattus norvegicus (Rat)

TUBULIN BETA CHAIN.

[MASS = 49963

2
2

Rattus norvegicus (Rat)

LEUKOCYTE COMMON

ANTIGEN-RELATED

PHOSPHATASE

PRECURSOR.

[MASS = 212954

2
2

Rattus norvegicus (Rat)

PREDICTED similar to

POSTSYNAPTIC

DENSITY PROTEIN.

[MASS = 186848

2
2

Rattus norvegicus (Rat)

COMPLEMENT

INHIBITORY FACTOR H.

[MASS = 140344

2
2

Rattus norvegicus (Rat)

PROTEASOME SUBUNIT

BETA TYPE 2.

[MASS = 22912

2
2

Rattus norvegicus (Rat)

40S RIBOSOMAL

PROTEIN S11.

[MASS = 18431

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO EIF4G1 PROTEIN.

[MASS = 175705

2
2

Rattus norvegicus (Rat)

92 KDA PROTEIN.

[MASS = 91785

2
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO RANBP4.

[MASS = 118926

2
7

Rattus norvegicus (Rat)

BETA-ENOLASE.

[MASS = 46830

2
2

Rattus norvegicus (Rat)

PEROXIREDOXIN-1.

[MASS = 22109

2
3

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO APOLIPOPROTEIN

C2. [MASS = 10695

2
2

Rattus norvegicus (Rat)

UDP-N-

ACETYLGLUCOSAMINE--

PEPTIDE N-

ACETYLGLUCOSAMINYL

TRANSFERASE 110 KDA

SUBUNIT.

[MASS = 116954

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO NUCLEAR PORE

COMPLEX-ASSOCIATED

INTRANUCLEAR

COILED-COIL PROTEIN

TPR. [MASS = 279947

2
2

Rattus norvegicus (Rat)

PROTEASOME SUBUNIT

ALPHA TYPE 6.

[MASS = 27399

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO HCF. [MASS = 215082

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO SHPRH PROTEIN.

[MASS = 192457

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO 60S RIBOSOMAL

PROTEIN L12.

[MASS = 17847

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO TRANSLIN-

ASSOCIATED FACTOR X

(TSNAX) INTERACTING

PROTEIN 1.

[MASS = 85228

2
2

Rattus norvegicus (Rat)

JUNCTION

PLAKOGLOBIN.

[MASS = 81801

2
5

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO GLYCERALDEHYDE-

3-PHOSPHATE

DEHYDROGENASE.

[MASS = 35856

2
2

Rattus norvegicus (Rat)

PREDICTED: SIMILAR

TO DESMOPLAKIN

ISOFORM II.

[MASS = 264186

2
2

Rattus norvegicus (Rat)

GM2 GANGLIOSIDE

ACTIVATOR PROTEIN.

[MASS = 21493

2
2

Rattus norvegicus (Rat)

KINESIN-LIKE PROTEIN

KIF15. [MASS = 159554

2
2

Rattus norvegicus (Rat)

29 KDA PROTEIN.

[MASS = 29242

2
2

Rattus norvegicus (Rat)

RIBOSOMAL PROTEIN

L13A. [MASS = 23446

2
2

Rattus norvegicus (Rat)

60S RIBOSOMAL

PROTEIN L8.

[MASS = 27893

2
2

Rattus norvegicus (Rat)

PREDICTED: TYROSINE

KINASE RECEPTOR 1.

[MASS = 125210

Parada et al (J Proteome Res, 2005. 4:2420-8) identified 31 proteins within the rat e-CSF finding an abundance of extracellular matrix proteins, enzymes, and enzyme regulators, consistent with our study. We identified a much larger number of proteins within the CSF while identifying 24 of the 31 previously identified proteins. The 7 proteins that we did not find are the following: calreticulin, DJ-1, EEf1 g, laminin receptor 1, malate dehydrogenase 1, set beta isoform, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta polypeptide. The differences between our study and the study by Parada et al appear a consequence of methodology rather than sample differences. Parada et al chose for mass spectrometry the most prominent silver-stained e-CSF proteins resolved by two-dimensional electrophoresis, whereas we performed an analysis of the entire e-CSF separated by one-dimensional electrophoresis. Our one-dimensional approach enabled a more comprehensive analysis (one which would be unwieldy for an entire two dimensional gel), the study by Parada et al is complementary with this one as some proteins resolved in two dimensions would have a reduced likelihood of becoming suppressed due to co-migration in one dimension with abundant protein species such as albumin.

Our analyses are semi-quantitative, and we identified interesting differences between our various rat e-CSF samples. Apolipoprotein M is found in both E14.5 LV and E14.5 4thV but our analysis did not identify it in either E12.5 LV or E17.5 LV, phosphatidylethanolamine binding protein was found only in the E17.5 LV, collagen alpha I (XI) was identified in E14.5 and E17.5 LV, and phosphatase 2 (alpha isoform of regulatory subunit A) was found in E12.5 LV. Also, apolipoprotein D, an apolipoprotein that was not identified by Parada et al was identified only in the E14.5 4^thV.

Comparison of Human and Rat CSF

In a comparison of proteins found in the human e-CSF to the proteins found in the rat e-CSF, we found that of the 188 proteins identified in the human e-CSF, 135 human proteins were identified in any one of the four samples of embryonic rat CSF. 83 of those proteins were present in all four samples of embryonic rat CSF. Table 1 includes the human proteins found common to rat CSF. We have indicated the specific rat samples in which each protein was identified. Out of the top 50 proteins found in the human CSF, 45 were also found in the rat CSF.

Proteins common to human and rat CSF presumably represent proteins related to fundamental CSF functions. For example, e-CSF contains many transport and carrier proteins including transferrin, albumin, alpha-fetoprotein, transthyretin, ceruloplasmin, and plasma retinol-binding protein that are all involved in either metal ion or vitamin transport through fluid or across cell membranes. There are a number of apolipoproteins involved in the transport and metabolism of lipids and fatty acids in the CSF as reported in this paper and by Parada et al (supra). There are also a large number of enzymes and protease inhibitors in the CSF that are involved in regulating immune response and maintaining homeostasis.

Other proteins common to rat and human CSF may play more specific roles in neurogenesis. One factor in the e-CSF is Amyloid Beta A4 Protein Precursor (APP), which we identified in rat CSF at E12.5, E14.5, and E17.5 and human CSF at CS20. This protein is normally present in brain and a soluble form is known to circulate in adult CSF (Palmert et al., Proc Natl Acad Sci USA, 1989. 86:6338-42). The soluble form of APP has been shown to stimulate proliferation of embryonic neural stem cells as well as adult neural progenitor cells from the subventricular zone (Caille et al., Development, 2004. 131:2173-81; Hayashi et al., Biochem Biophys Res Commun, 1994. 205:936-43; Ohsawa et al., Eur J Neurosci, 1999. 11:1907-13). APP may play a role during neurogenesis not only within the cell but may be released in the extracellular space and taken up in the CSF in order to diffuse throughout the CSF a play a function at more distant sites. Similarly, Tenascin, which we found in all CSF samples from rat and human from CS 20, is a secreted extracellular matrix glycoprotein implicated in axon guidance during development and regeneration (von Hoist et al., J Biol Chem, 2007. 282: 9172-81), which was recently shown to be expressed in progenitor cells in the ventricular zone of the developing brain. CSF contains multiple critical extracellular matrix factors including fibronectin, laminin, tenascin, fibulin, versican, and neurocan core protein. Because many of these factors can support or orient neuronal migration, they may be acting in the CSF as external cues for proliferating and differentiating neuronal progenitor cells.

Few proteins were identified that may be exclusive to rat or human e-CSF. The protein Pigment Epithelium Derived Factor (PEDF) was only found in the human e-CSF and is known to circulate in the adult CSF and is significantly decreased in CSF of patients with frontotemporal dementia (Davidsson et al., Brain Res Mol Brain Res, 2002. 109:128-33). This secreted serine protease inhibitor, known to be released by retinal pigment cell into the matrix, is a known neurotrophic protein involved in survival and potentially differentiation of specific neurons (Houenou et al., J Comp Neurol, 1999. 412:506-14). PEDF is known to act on photoreceptor cells but also may play a role in spinal motor neuronal survival. It is likely that PEDF is released by the photoreceptor cells into the matrix and taken up by the CSF and may act on cell types and neurons by diffusion through the CSF. Similarly, the Neuronal Cell Adhesion Molecule L1-Like Protein, also found only in the human e-CSF is known to play important roles in neurite outgrowth and neuronal survival (Hillenbrand et al., Eur J Neurosci, 1999. 11:813-26; Montag-Sallaz et al., Mol Cell Biol, 2002. 22:7967-81; Nishimune et al., J Neurosci Res, 2005. 80:593-9).

Conversely, we only observed the Extracellular Superoxide Dismutase, a protein known to remove free radicals that can be toxic to cells in rat e-CSF. One of the functions of the e-CSF may be the removal of toxins and toxin metabolic byproducts, and therefore it would important to have proteins within the CSF that help neutralize some of the toxic products released into the CSF. Additionally, we found in the rat e-CSF Mannose 6-phosphate/Insulin-like Growth Factor II Receptor (IGF2R), a soluble form of the receptor has been found in the serum, amniotic fluid and urine of both rodents and humans, affecting organ size based on its interaction with IGF2 and other factors (Causin et al., Biochem J, 1988. 252:795-9; Kiess et al., Proc Natl Acad Sci USA, 1987. 84:7720-4; MacDonald et al., J Biol Chem, 1989. 264:3256-61; Xu et al., J Clin Endocrinol Metab, 1998. 83:437-42; Zaina et al., J Biol Chem, 1998. 273:28610-6). Confirmation of these apparent differences would require Western blotting, and may lead to studies of their intriguing biological potential in the e-CSF.

Subcellular Localization of e-CSF Proteins

To compare the e-CSF of human and rat further we analyzed the 188 proteins found in the human e-CSF and the 137 proteins in the rat e-CSF present in all samples based on subcellular localization, molecular function, and biological process. The subcellular localization of each protein in the CSF is shown in Tables 1 and 3. The majority of proteins found in the human (FIG. 2A) and rat (FIG. 2B) e-CSF are secreted proteins which compose 27% and 33% of the total proteins found within the CSF respectively. The second most common localization of proteins found in the e-CSF of both humans and rats are cell membrane proteins, composing 20% and 18% respectively. The relatively high percentage of membrane proteins is consistent with the recent discovery of membrane bound particles in the CSF (Marzesco et al., J Cell Sci, 2005. 118:2849-58). Out of 188 proteins found in the human e-CSF, 19% are cytoplasmic proteins, 16% are secreted proteins found in the extracellular space or extracellular matrix (ECM), 14% are nuclear proteins, and 9% are intracellular proteins that could not be specifically localized to one compartment. Out of 137 proteins present in all rat e-CSF samples 14% are cytoplasmic proteins, 15% are ECM proteins, 3% are nuclear proteins, and 12% are intracellular proteins. As a control to assess subcellular localization in a protein population of embryonic brain, we chose to analyze the top 200 proteins identified from E16.5 mouse forebrain and midbrain in a previous study (Ballif et al., Mol Cell Proteomics, 2004. 3:1093-101). FIG. 2C shows that 42% of these proteins are in the cytoplasm, 22% nuclear, 14% intracellular, 7% at the cell membrane, and 7% mitochondrial. Strikingly no mitochondrial proteins were found in the CSF. Out of the 200 proteins analyzed from embryonic mouse brain, two are secreted and three are found in the extracellular space or matrix. FIG. 2D shows a graphical representation of the comparison of embryonic human CSF, rat CSF and mouse brain based on localization. The e-CSF as compared to brain tissue clearly has an abundant number of secreted proteins, extracellular matrix proteins, and cell membrane proteins as opposed to an overwhelming majority of cytoplasmic, nuclear, and mitochondrial proteins found in the brain tissue.

Molecular Function and Biological Process

We used the PANTHER protein ontology database to classify the proteins into distinct categories of molecular function and biological process. Panther identified 180 out of 188 proteins with a total number of 237 functional hits for the human e-CSF, 119 out of 137 proteins with a total number of 155 functional hits for the rat e-CSF, and 191 out of 200 proteins with a total number of 234 functional hits for embryonic mouse brain. Table 1 shows the percentage of proteins assigned to each functional category in the embryonic human CSF, embryonic rat CSF, and E16.5 embryonic mouse brain. FIGS. 5A-5C represent functional classification of the samples as individual pie charts including the absolute number of proteins assigned to each function group.

TABLE 5

List of protein categories based on molecular function for embryonic

human CSF, rat CSF and mouse brain.

Percent

Percent

Percent

proteins

proteins

proteins

in each

in each

in each

Human CSF
category
Rat CSF
category
Mouse brain
category

Cell adhesion
11.1%
Cell adhesion
12.6%
Cell adhesion
2.60%

Chaperone
5.0%
Chaperone
5.0%
Chaperone
8.40%

Cytoskeletal
7.2%
Cytoskeletal
8.4%
Cytoskeletal
11.50%

Defense/Immunity
8.3%
Defense/Immunity
6.7%
Defense/Immunity
0.00%

Extracellular matrix
15.6%
Extracellular matrix
10.9%
Extracellular matrix
0.50%

Hydrolase
2.2%
Hydrolase
1.7%
Hydrolase
6.30%

Kinase
1.1%
Kinase
2.5%
Kinase
2.60%

Ligase
0.6%
Ligase
0.8%
Ligase
3.70%

Membrane traffic
1.1%
Membrane traffic
0.8%
Membrane traffic
2.60%

Miscellaneous
4.4%
Miscellaneous
3.4%
Miscellaneous
2.60%

Unclassified
7.2%
Unclassified
5.9%
Unclassified
14.70%

Nucleic acid
10.0%
Nucleic acid binding
5.0%
Nucleic acid binding
18.30%

binding

Oxidoreductase
2.8%
Oxidoreductase
5.0%
Oxidoreductase
4.70%

Phosphatase
1.1%
Phosphatase
2.5%
Phosphatase
1.60%

Protease
7.2%
Protease
6.0%
Protease
1.60%

Receptor
7.8%
Receptor
10.1%
Receptor
2.10%

Calcium binding
2.8%
Calcium binding
4.2%
Calcium binding
3.70%

Regulatory
13.3%
Regulatory molecule
12.6%
Regulatory molecule
8.40%

molecule

Signaling molecule
6.1%
Signaling molecule
6.0%
Signaling molecule
1.60%

Synthase and
0.6%
Synthase and
1.0%
Synthase and
2.60%

synthetase

synthetase

synthetase

Transcription factor
1.1%
Transcription factor
1.0%
Transcription factor
3.70%

Transfer/Carrier
8.3%
Transfer/Carrier
12.6%
Transfer/Carrier
3.70%

Transferase
1.7%
Transferase
1.0%
Transferase
4.70%

Transporter
3.9%
Transporter
3.4%
Transporter
4.70%

Cell junction
1.1%
Cell junction protein
0%
Cell junction protein
0%

protein

Lyase
0%
Lyase
0%
Lyase
1.60%

Ion channel
0%
Ion channel
0%
Ion channel
1.60%

Isomerase
0%
Isomerase
1.7%
Isomerase
1.00%

Panther analysis of molecular function reveals the majority of proteins found within the human and rat CSF share similar functional categories (Table 4, FIG. 3, and FIGS. 5A-5C). Proteins involved in extracellular matrix function make up, respectively, 16% and 11% of the majority of proteins found in the e-CSF of humans and rats. Other abundant categories of proteins found in the e-CSF include regulatory molecules such as protease inhibitors (human-13%, rat-13%), cell adhesion proteins (human-11%, rat-13%), nucleic acid binding proteins (human-10%, rat-5%), transfer/carrier proteins (human-8%, rat-13%), immune defense proteins (human-8%, rat-7%), and receptors (human-8%, rat-10%). The total number of enzymes also is a large component of the CSF. The embryonic human CSF has a total of 28 different functional enzymes (16%) and embryonic rat CSF has a total of 23 different functional enzymes (19%). Furthermore, the e-CSF is composed of a large number of different enzyme classes, and is particularly high in proteases (human-7%, rat-6%), and oxidoreductases (human-3%, rat-5%).

Panther analysis reveals distinct functional groups of proteins present in the CSF as compared to embryonic tissue. Protein categories in the embryonic human and rat CSF are quite similar and to control for random similarity in categorization based on molecular function we compared the CSF protein samples to a sample of 200 most abundant proteins in embryonic E16.5 mouse brain (Table 4). The comparison of relevant protein categories in each sample is shown in FIG. 3. The two largest categories of proteins in the embryonic mouse brain include nucleic acid binding proteins (18.3%) and cytoskeletal proteins (11.5%). Interestingly, proteins involved in defense and immunity which comprised 7-8% of e-CSF were completely absent from the top 200 proteins in the embryonic mouse brain sample. One category of proteins that appears to be similar in all three comparisons is the regulatory molecules (13.3% in human CSF, 12.6% in rat CSF, and 8.4% in mouse brain). We further classified the regulatory molecules into smaller categories and although the larger classification shows similar percentages of regulatory molecules, the sub-classification clearly distinguishes the e-CSF samples from the embryonic brain sample (FIG. 7). The majority of proteins in the e-CSF within the regulatory molecule class are sub-classified as protease inhibitors comprising 75% and 87% of proteins within the class in human and rat CSF respectively as compared to 0% in the mouse brain (FIG. 7). Based on molecular function the most abundant classes of protein present in the e-CSF are found to be proteins of the extracellular matrix, regulatory molecules, transfer/carrier proteins, cell adhesion proteins, and proteins involved in immunity and defense.

Panther analysis of proteins based on biological process reveals strong similarity between the embryonic human and rat CSF and differences between the CSF and the embryonic brain (Table 6, FIG. 4, and FIGS. 6A-6C). The five most abundant classes in both embryonic human and rat CSF are protein metabolism and modification, signal transduction, immunity and defense, cell adhesion, and developmental processes. The majority of proteins in the analysis of the embryonic mouse brain are involved in protein metabolism and modification, nucleic acid metabolism, intracellular protein traffic, cell cycle, and cell structure and motility. Comparing the analysis of the mouse brain with the e-CSF shows that the CSF samples contain proteins that are enriched for a number of various biological processes that are distinct from that of embryonic brain tissue (FIG. 4). Interestingly, all three samples are most abundant in proteins involved in protein metabolism and modification (FIG. 4). However, Panther analysis shows that CSF and brain show different types of proteins even among the same overall class (FIG. 8). Sub-classification of this category reveals the majority of proteins in the mouse brain involved in protein biosynthesis (30%) and protein modification (28%) with only 19% of proteins involved in proteolysis (FIG. 8). However in both the human and rat e-CSF the overwhelming majority of proteins in both samples are involved in proteolysis comprising 58% in humans and 54% in rats (FIG. 8). This class of biological processes includes the large number of protease inhibitors and proteases found within the CSF.

TABLE 6

List of protein categories based on biological process for

embryonic human CSF, rat CSF and mouse brain.

Percent

Percent

proteins in

proteins in

Percent

each

each

proteins in each

Human CSF
category
Rat CSF
category
Mouse brain
category

Neuronal
0.6%
Neuronal
0.8%
Neuronal
1.60%

activities

activities

activities

Signal
25.0%
Signal
26.1%
Signal
8.90%

transduction

transduction

transduction

Developmental
16.1%
Developmental
16.8%
Developmental
7.30%

processes

processes

processes

Cell proliferation
4.4%
Cell proliferation
6.7%
Cell proliferation
3.70%

and differentiation

and differentiation

and

differentiation

Coenzyme and
0.6%
Coenzyme and
1.7%
Coenzyme and
1.60%

prosthetic group

prosthetic group

prosthetic group

metabolism

metabolism

metabolism

Cell structure and
13.9%
Cell structure and
16.0%
Cell structure and
10.50%

motility

motility

motility

Immunity and
22.2%
Immunity and
18.5%
Immunity and
4.20%

defense

defense

defense

Apoptosis
2.8%
Apoptosis
2.5%
Apoptosis
3.10%

Oncogenesis
2.2%
Oncogenesis
3.4%
Oncogenesis
2.10%

Muscle
0.6%
Muscle
0.8%
Muscle
0.50%

contraction

contraction

contraction

Transport
8.9%
Transport
15.1%
Transport
9.40%

Blood circulation
5.0%
Blood circulation
5.9%
Blood circulation
0.50%

and gas exchange

and gas exchange

and gas exchange

Carbohydrate
1.1%
Carbohydrate
1.7%
Carbohydrate
3.70%

metabolism

metabolism

metabolism

Nucleoside,
10.6%
Nucleoside,
5.0%
Nucleoside,
18.80%

nucleotide and

nucleotide and

nucleotide and

nucleic acid

nucleic acid

nucleic acid

metabolism

metabolism

metabolism

Homeostasis
0.6%
Homeostasis
2.5%
Homeostasis
1.60%

Protein
27.8%
Protein
27.7%
Protein
24.60%

metabolism and

metabolism and

metabolism and

modification

modification

modification

Cell cycle
6.7%
Cell cycle
7.6%
Cell cycle
11.00%

Intracellular
9.4%
Intracellular
11.8%
Intracellular
13.10%

protein traffic

protein traffic

protein traffic

Cell adhesion
20.0%
Cell adhesion
17.6%
Cell adhesion
1.60%

Lipid, fatty acid
3.3%
Lipid, fatty acid
5.9%
Lipid, fatty acid
3.10%

and steroid

and steroid

and steroid

metabolism

metabolism

metabolism

Sensory
1.1%
Sensory
1.7%
Sensory
0.50%

perception

perception

perception

Electron transport
0.6%
Electron transport
0.8%
Electron
1.00%

transport

Amino acid
0.6%
Amino acid
0.8%
Amino acid
1.00%

metabolism

metabolism

metabolism

Biological process
5.0%
Biological process
5.0%
Biological
15.20%

unclassified

unclassified

process

unclassified

Protein targeting
2.2%
Protein targeting
2.5%
Protein targeting
4.20%

and localization

and localization

and localization

Miscellaneous
1.1%
Miscellaneous
0.8%
Miscellaneous
1.60%

Phosphate
0.0%
Phosphate
0.0%
Phosphate
0.50%

metabolism

metabolism

metabolism

Other metabolism
0.0%
Other metabolism
0.0%
Other
1.00%

metabolism

The similarities between the embryonic human and rat CSF are apparent when the proteins are classified into groups and analyzed on the basis of subcellular localization, molecular function, and biological process. Based on the functional characteristics of the proteins found in the e-CSF, the CSF is a heterogeneous mixture of many types of classes of proteins with varying functions. The e-CSF is far more complex than previously thought. This may be due to active secretion from the choroid plexus into the CSF, or from the contents within the extracellular membrane bound particles that are present in the rodent CSF during development, or potentially to aposomes budding from the choroid plexus and floating within the CSF that have been shown previously to support protein translation (Saunders et al., Cell Mol Neurobiol, 2000. 20:29-40; Agnew et al., Cell Tissue Res, 1980. 208:261-81; Gudeman et al., J Neurosci Res, 1989. 24:184-91).

Although we did not find the growth factor FGF-2 as reported by Martin et al (Dev Biol, 2006. 297:402-16), many growth factors are in low abundance and are of smaller molecular weight making them more challenging to identify by multiple peptide assignments using mass spectrometry on a complex mixture.

Cortical Explants in e-CSF

Cortical explants can survive and proliferate in the presence of e-CSF. Embryonic day 16 (E16) rat cortical explants cultured with 100% E17 CSF for 24 hours, without additional exogenous media or factors, retained tissue architecture, cell proliferation, and cell viability, approximating in vivo E17 rat cortex (FIGS. 9B, 9C, 9E, and 9F). In contrast, culturing E16 explants with 100% artificial CSF (ACSF) failed to maintain the integrity of the embryonic cortical tissue, as reflected by decreased proliferation and mitotic activity, disorganized neuronal morphology, and a striking increase in cell death (FIGS. 9D and 9G and FIGS. 10A-10F). Thus, the embryonic CSF proteome provides an endogenous signaling milieu of essential growth and survival factors for the developing cortex.

Comparison of E13 and E17 Rat e-CSF

The primary source of CSF is the choroid plexus, a highly vascularized secretory epithelial tissue that extends into the ventricles. To determine if the embryonic choroid-plexus-derived-CSF provides support and instructive cues to the developing cortex we compared CSF from E13 embryos (pre choroid plexus formation) with that from E17 embryos (post choroid plexus formation). E17 CSF increased the frequency of PH3-labeled proliferating cells in E16 cortical explants compared to explants cultured with E13 CSF (E17 mean: 44.1±±1.43; E13 mean: 25±4.2; n=4; p<0.05) (FIGS. 9H, 9I, and 9N) with a greater than 2.5-fold increase in PH3-positive-staining cells along the ventricular zone (VZ) (E17 mean: 32.3±0.79; E13 mean: 12.8±3.9; n=4, p<0.05) (FIG. 9O). To determine the identity of mitotic cells, explants were stained with an anti-phosphorylated Vimentin 4A4 antibody, an established marker of proliferating neural progenitor cells (Anthony et al., Neuron 41, 881-90 (2004); Weissman et al., Cereb Cortex 13, 550-9 (2003)). E16 explants cultured in E17 CSF revealed increased Vimentin 4A4-positive-staining cells per explant compared to explants grown in E13 CSF (E17 mean: 38.4±1.1; E13 mean: 13.9±2.2; n=3, p<0.05) (FIG. 1j, k, p). No difference in the number of Tbr2-positive-staining cells undergoing division was observed (data not shown). Taken together, these data suggest that age-dependent differences in embryonic CSF signals are both supportive and instructive for precursor proliferation in the developing cortex.

CSF was then determined to maintain and stimulate proliferation of primary dissociated cortical progenitors cultured as neurospheres, an in vitro experimental model for neural stem cells. Primary neurospheres derived from E14 rat embryos were dissociated, plated at clonal density, and cultured with CSF collected from E13 or E17 embryos. Both E13 and E17 CSF supported the generation of small neurospheres composed primarily of GLAST-positive-staining cells in the absence of supplemental FGF and EGF for 10 days in vitro (DIV) (FIGS. 9Q-9Y). Neurospheres failed to form in the presence of ACSF. Consistent with our explant experiments, cells cultured in E17 CSF generated not only increased numbers of neurospheres (FIG. 9Z), but also larger spheres (data not shown), indicating that E17 CSF contains instructive proliferative signals. In addition, neurospheres grown in CSF retained responsiveness to FGF and EGF, indicating that the CSF is maintaining the stem cells in an uncommitted fate (FIGS. 11A-11I).

Both E13 and E17 CSF maintain viable GLAST-positive-staining neurospheres (FIGS. 12A-12G) after 44 DIV, while E17 CSF promoted the survival of an increased number of neurospheres compared to E13 CSF. Thus, embryonic CSF is sufficient for maintaining and stimulating proliferating cortical progenitor cells.

We next characterized the embryonic CSF proteome to determine how the CSF drives the proliferation of cortical progenitor cells. Total CSF protein concentration increased from E12 on, peaked at birth (P0) and declined into adulthood (FIG. 13A). We visualized the overall protein composition of CSF by silver staining, and observed a graded transition of CSF constituents from E13 to adulthood (FIG. 13B). Immunoblot analysis of proteins identified by tandem mass spectrometry (LC-MS/MS) (Zappaterra et al., J Proteome Res 6, 3537-48 (2007)) revealed dynamic changes in different classes of proteins in CSF during development (FIG. 13C and data not shown). For example, several proteins known to regulate proliferation of neural progenitors including transferrin, cystatin C, FGF2, and soluble isoforms of amyloid precursor protein (sAPP) were expressed throughout development and, in some cases, in the adult CSF (FIG. 13C). Other proteins involved in tissue homeostasis, such as the antioxidant and free radical scavenger extracellular super oxide dismutase (EC-SOD, Sod3), and the protease Cathepsin B were robustly expressed early in development and rapidly downregulated thereafter (FIG. 13C). Together with the CSF proteome, these proteins contribute to the role of CSF in development by providing essential growth-promoting cues to the developing cortex.

To investigate the distinct effects of embryonic CSF at different developmental stages, we performed extensive LC-MS/MS analyses on increased volumes of E17 CSF. From these E17 rat proteome analyses, we identified several peptides corresponding to Insulin-like growth factor 2 (IGF2) in the CSF (FIG. 14A and Table 7). IGF2 is a particularly compelling CSF resident protein given the crucial role of IGF signaling in prenatal growth and brain size, as well as in regulating neural progenitor cell division (Randhawa et al., Mol Genet Metab 86, 84-90 (2005); Hodge et al., J Neurosci 24, 10201-10 (2004); Baker et al., Cell 75, 73-82 (1993)). IGF2 is also essential in the embryonic stem (ES) cell niche (Bendall et al., Nature 448, 1015-21 (2007)). Interestingly, we found that IGF2 is transiently expressed in the CSF during development. IGF2 was first detected at E13 and maximally expressed during cortical neurogenesis (E15-E19), after which its expression declined postnatally (FIG. 14B). The dynamic availability of Igf2 in the embryonic CSF raised the possibility that IGF signaling may contribute to the differential capacity of embryonic CSF between E13 and E17 to support cortical neural progenitor proliferation (Bendall et al., supra). We therefore sought to characterize the role IGF2 in neural development, as outlined below.

TABLE 7

Protein
MW

14-3-3 protein
28037

beta/alpha

14-3-3 protein
29345

epsilon

14-3-3 protein
28342

gamma

14-3-3 protein
28063

theta

14-3-3 protein
27942

zeta/delta

18 kDa protein
18663

20 kDa protein
20033

21 kDa protein
21585

26S protease
45797

regulatory

subunit 8

29 kDa protein
29540

31 kDa protein
31415

32 kDa protein
32525

32 kDa protein
32833

33 kDa protein
32943

35 kDa protein
35005

35 kDa protein
35717

35 kDa protein
35586

38 kDa protein
38434

38 kDa protein
38434

38 kDa protein
38510

39 kDa protein
39428

40S ribosomal
18602

protein S11

40S ribosomal
26845

protein S3

40S ribosomal
30042

protein S3a

40S ribosomal
29694

protein S4, X

isoform

40S ribosomal
22918

protein S5

40S ribosomal
28851

protein S6

40S ribosomal
22127

protein S7

40S ribosomal
24359

protein S8

40S ribosomal
32807

protein SA

42 kDa protein
42846

43 kDa protein
45093

45 kDa protein
44917

45 kDa protein
45796

45 kDa protein
45796

45 kDa protein
45796

46 kDa protein
46534

47 kDa heat
46631

shock protein

precursor

50 kDa protein
50675

50 kDa protein
50450

51 kDa protein
51503

60S acidic
34386

ribosomal

protein P0

60S ribosomal
20349

protein L11

60S ribosomal
24129

protein L15

60S ribosomal
34555

protein L5

60S ribosomal
30386

protein L7

90 kDa protein
90936

Aa1064
538071

Ab2-076
104278

Ab2-379
62360

Ac1873
87398

Actin, alpha
42393

skeletal muscle

Actin, gamma-
42276

enteric smooth

muscle

Adenine
20049

phosphoribosyltransferase

Adenosylhomocysteinase
47920

ADP-ribosylation
20526

factor 3

ADP-ribosylation
20379

factor 4

ADP-ribosylation
20065

factor 6

Alcohol
36602

dehydrogenase

Aldose
36122

reductase

Alpha-1-
46306

antiproteinase

precursor

Alpha-2
46522

antiplasmin

Alpha-2-globin
15456

chain

Alpha-2-HS-
38781

glycoprotein

precursor

AMBP protein
39763

precursor

AMBP protein
39763

precursor

Angiotensin II
57615

type 1A receptor

associated

protein

Angiotensinogen
52209

precursor

APEX
32752

Apolipoprotein
30119

A-I

Apolipoprotein
11496

A-II precursor

Apolipoprotein
44456

A-IV precursor

Apolipoprotein E
35810

precursor

Apolipoprotein M
21855

precursor

Ba1-667
109614

Beta-1,3-N-
42471

acetylglucosaminyl-

transferase lunatic

fringe

Beta-2-glycoprotein I
34338

precursor

Beta-2-microglobulin
13834

precursor

Calcyclin binding
26655

protein

cAMP-dependent
43191

protein kinase type I-

alpha regulatory

subunit

Carbonic anhydrase 2
29153

Casein kinase II,
45187

alpha chain

Cathepsin B,
38457

preproprotein

Cathepsin L
38231

precursor

Cathepsin Z
34879

precursor

Cd81 protein
26589

Cell division control
34191

protein 2 homolog

Cfh protein
46732

Cofilin-1
25102

Collagen alpha 1(V)
185292

chain precursor

Collagen alpha 1(XI)
171893

chain precursor

Collagen alpha 2(I)
130076

chain precursor

Collagen alpha 2(I)
130076

chain precursor

Collagen alpha1
138912

Complement C1q
26817

subcomponent, B

chain precursor

Complement C1q
25971

subcomponent, C

chain precursor

Connective tissue
39981

growth factor

precursor

Creatine kinase B-
42997

type

Cystatin C
15665

Cytosolic malate
36654

dehydrogenase

DJ-1 protein
20202

EGF-containing
46847

fibulin-like

extracellular matrix

protein 2

Elongation factor 1-
50456

alpha 1

Epsilon 1 globin
16161

Epsilon-sarcoglycan
50239

Eukaryotic
36262

translation initiation

factor 2 subunit 1

Eukaryotic
40076

translation initiation

factor 3, subunit 3

gamma, 40 kDa

Extracellular
27019

superoxide

dismutase [Cu—Zn]

precursor

FAM3C-like protein
24999

Fatty acid-binding
15018

protein, brain

Fetuin-B precursor
42388

Fructose-
39677

bisphosphate

aldolase A

Fructose-
39551

bisphosphate

aldolase C

Galactokinase 1
42832

Gelsolin
86856

Glutathione S-
23536

transferase P

Glyceraldehyde-3-
36079

phosphate

dehydrogenase

GTP-binding nuclear
24594

protein Ran

GTP-binding nuclear
24622

protein Ran, testis-

specific isoform

Guanine nucleotide-
35875

binding protein beta

subunit 2-like 1

Guanine nucleotide-
38044

binding protein

G(I)/G(S)/G(T) beta

subunit 1

Hemoglobin alpha-1
15368

and alpha-2 chains

Hemoglobin beta
15962

chain, major-form

Hemoglobin beta
15965

chain, minor-form

Heterogeneous
34252

nuclear

ribonucleoprotein A1

Heterogeneous
32914

nuclear

ribonucleoprotein C

Heterogeneous
46072

nuclear

ribonucleoprotein F

Hypothetical
32346

LOC315594

Hypothetical
35039

LOC316842

Hypothetical
33553

LOC363644

Hypothetical protein
59591

Hypothetical protein
37353

Hypothetical protein
34736

Hypothetical protein
34748

LOC311078

Hypothetical protein
33918

RGD1308228_predicted

HYRAC
32094

Ig lambda-2 chain C
25750

region

Insulin-like growth
33966

factor binding protein

2 precursor

Insulin-like growth
28886

factor binding protein

4 precursor

Insulin-like growth
20542

factor II precursor

Isocitrate
47076

dehydrogenase

[NADP] cytoplasmic

Lactadherin
48553

precursor

LIM and SH3
30369

domain protein 1

L-lactate
36735

dehydrogenase A

chain

L-lactate
36766

dehydrogenase B

chain

LOC367586
51633

protein

LOC500183
26034

protein

LRRGT00147
108859

Lysozyme
17186

Mannose-binding
81703

protein

associated serine

protease-1

Masp1 protein
45168

Matrix Gla-protein
12208

precursor

Metalloproteinase
24478

inhibitor 1

precursor

Metalloproteinase
25041

inhibitor 2

precursor

Multifunctional
47706

protein ADE2

Neurexin-2-alpha
187392

precursor

Neutrophil
10452

antibiotic peptide

NP-4 precursor

NG,NG-
31694

dimethylarginine

dimethylaminohydrolase 1

Nuclear migration
38412

protein nudC

Pancreatic
52181

triacylglycerol

lipase precursor

Peptidyl-prolyl cis-
17971

trans isomerase A

Peptidyl-prolyl cis-
23859

trans isomerase

B precursor

Peptidylprolyl
23066

isomerase C

Peroxiredoxin 1
22337

Peroxiredoxin 2
21823

Phosphatidylethanolamine-
20784

binding

protein

Phosphoglycerate
44937

kinase 1

Phosphoglycerate
45409

kinase 2

Phosphoglycerate
28571

mutase 1

Phosphoglycerate
28795

mutase 2

Phosphoserine
40969

aminotransferase 1

Plasma
25678

glutathione

peroxidase

precursor

Platelet-activating
47109

factor

acetylhydrolase

IB alpha subunit

PREDICTED:
66492

ATPase, H+

transporting,

lysosomal

accessory protein 2

PREDICTED:
97048

dystroglycan 1

PREDICTED:
73357

eukaryotic

translation

elongation factor

1 gamma

PREDICTED:
44883

Glycoprotein-4-

beta-

galactosyltransferase 2

PREDICTED:
73188

multiple inositol

polyphosphate

histidine

phosphatase 1

PREDICTED:
176810

nidogen 2

(predicted)

PREDICTED:
182884

procollagen type

XI alpha 1

PREDICTED:
37413

proteasome

(prosome,

macropain)

subunit, beta type 5

PREDICTED:
50766

retinol binding

protein 4, plasma

PREDICTED:
62084

serine (or

cysteine)

proteinase

inhibitor, clade F,

member 2

(predicted)

PREDICTED:
25236

similar to 25 kDa

FK506-binding

protein

PREDICTED:
22761

similar to 40S

ribosomal protein

S9

PREDICTED:
25560

similar to 60S

ribosomal protein

L7

PREDICTED:
28385

similar to 60S

ribosomal protein

L8

PREDICTED:
21971

similar to 60S

ribosomal protein

L9

PREDICTED:
79786

similar to Ab2-076

PREDICTED:
39999

similar to actin

related protein ⅔

complex subunit 2

PREDICTED:
62610

similar to Actin,

cytoplasmic 2

(Gamma-actin)

PREDICTED:
370838

similar to alpha 3

type VI collagen

isoform 1

precursor

PREDICTED:
47602

similar to Alpha

enolase (2-

phospho-D-

glycerate hydro-

lyase) (Non-

neural enolase)

(NNE)

PREDICTED:
46831

similar to Alpha

enolase (2-

phospho-D-

glycerate hydro-

lyase) (Non-

neural enolase)

(NNE)

PREDICTED:
23384

similar to alpha

NAC/1.9.2.

protein

PREDICTED:
51683

similar to Alpha-

centractin

(Centractin)

(Centrosome-

associated actin

homolog) (Actin-

RP

PREDICTED:
10695

similar to

Apolipoprotein C2

PREDICTED:
18823

similar to cofilin

PREDICTED:
194644

similar to collagen

alpha 2(IV) chain

precursor -

mouse

PREDICTED:
49756

similar to ELAV

(embryonic lethal,

abnormal vision,

Drosophila)-like 1

(Hu antigen R)

PREDICTED:
38691

similar to

Glyceraldehyde-

3-phosphate

dehydrogenase

(GAPDH)

PREDICTED:
22732

similar to

glyceraldehyde-3-

phosphate

dehydrogenase

(phosphorylating)

(EC 1.2.1.12) - m

PREDICTED:
54235

similar to

glycosyltransferase

28 domain

containing 1

PREDICTED:
506188

similar to heparan

sulfate

proteoglycan 2

(perlecan)

PREDICTED:
102890

similar to

heterogeneous

nuclear

ribonucleoprotein

A0

PREDICTED:
46478

similar to

heterogeneous

nuclear

ribonucleoprotein

D-like

PREDICTED:
40077

similar to

heterogeneous

nuclear

ribonucleoprotein

H3 isoform a

PREDICTED:
37486

similar to

Heterogeneous

nuclear

ribonucleoproteins

A2/B1 (hnRNP

A2/hnRNP B1)

PREDICTED:
25049

similar to High

mobility group

protein 1 (HMG-1)

(Amphoterin)

(Heparin-binding

protein p

PREDICTED:
29708

similar to IGFBP-

like protein

PREDICTED:
26598

similar to

immunoglobulin

light chain

PREDICTED:
64111

similar to keratin

6 alpha

PREDICTED:
151853

similar to

mKIAA1631

protein

PREDICTED:
307380

similar to myosin-

VIIb

PREDICTED:
298228

similar to

OTTHUMP00000060196

PREDICTED:
650022

similar to

OTTHUMP00000065631

PREDICTED:
43632

similar to

Phosphoglycerate

kinase 1

PREDICTED:
35269

similar to

poly(rC) binding

protein 2

PREDICTED:
38011

similar to

Poly(rC)-binding

protein 1 (Alpha-

CP1) (hnRNP-

E1)

PREDICTED:
117067

similar to

procollagen, type

IX, alpha 1

PREDICTED:
34868

similar to

Proteasome

subunit alpha

type 7-like

PREDICTED:
32587

similar to purine-

nucleoside

phosphorylase

PREDICTED:
33227

similar to

pyrophosphatase

PREDICTED:
35237

similar to RAB5B

protein

PREDICTED:
18522

similar to

ribosomal protein

L14

PREDICTED:
42305

similar to RIKEN

cDNA

4732495G21

gene

PREDICTED:
50964

similar to Tubulin

alpha-2 chain

(Alpha-tubulin 2)

PREDICTED:
44993

similar to tubulin,

beta, 2

PREDICTED:
40735

similar to

Vesicular

integral-

membrane

protein VIP36

precursor (Lectin,

mannose-binding

PREDICTED:
124213

thrombospondin 4

Proliferating cell
29091

nuclear antigen

Proliferation-
43999

associated 2G4,

38 kDa

ProProtein
82452

convertase

subtilisin/kexin

tyPe 9

Proteasome
25908

(Prosome,

macroPain)

subunit, beta

tyPe 4

Proteasome
25909

subunit alpha

type 2

Proteasome
28639

subunit alpha

type 3-like

Proteasome
29783

subunit alpha

type 4

Proteasome
27856

subunit alpha

type 6

Proteasome
26707

subunit beta type 1

Proteasome
23083

subunit beta type 2

Proteasome
25532

subunit beta type

6-like

Proteasome
30269

subunit beta type

7 precursor

Protein arginine
43063

N-

methyltransferase 1

Prothrombin
71837

precursor

Ras-related
23086

protein Rab-10

Ras-related
24471

protein Rab-11B

Ras-related
24155

protein Rab-14

Ras-related
23774

protein Rab-8B

Rho GDP
23464

dissociation

inhibitor (GDI)

alpha

Ribose-
35319

phosphate

pyrophosphokinase

I-like

Serine/threonine
36178

protein

phosphatase 2A,

catalytic subunit,

alpha isoform

Serine/threonine
38253

protein

phosphatase

PP1-alpha

catalytic subunit

Serum albumin
70715

precursor

Similar to Ras-
22348

related protein

Rab-1B

Soluble calcium-
45715

activated

nucleotidase 1

SPARC
35297

precursor

Splice Isoform 1
70211

of Alpha-

fetoprotein

precursor

Splice Isoform 1
276159

of Fibronectin

precursor

Splice Isoform 1
38363

of

Heterogeneous

nuclear

ribonucleoprotein

D0

Splice Isoform 1
44875

of Sex hormone-

binding globulin

precursor

Splice Isoform 3
214603

of Agrin

precursor

Splice Isoform
40889

Long of

Hyaluronan and

proteoglycan link

protein 1

precursor

Splice Isoform
28497

RC6-IL of

Proteasome

subunit alpha

type 7

Spliceosome
49491

RNA helicase

Bat1

Spp-24
23455

Stathmin
17157

Syntenin-1
32651

TGF-beta
95072

receptor type III

precursor

Thrombospondin 1
133664

Transcobalamin
47876

II precursor

Transforming
22124

protein RhoA

Translationally
19576

controlled tumor

protein

Transthyretin
15834

precursor

Triosephosphate
27303

isomerase

Tubb3 protein
50875

Tubulin alpha-1
50820

chain

Tubulin alpha-3
50644

chain

Tubulin beta-5
50127

chain

Tubulin, beta, 2
50257

Type A/B hnRNP
30967

p38

Ubiquitin
25180

carboxyl-terminal

hydrolase

isozyme L1

Ubiquitin-like 1
38969

Vascular
41763

endothelial cell

specific protein

11

Vimentin
53658

Vitamin D-
55141

binding protein

precursor

Zero beta-1
16079

globin

IGF2 in the CSF

To test if CSF could serve as a vehicle for IGF signaling, we assessed the expression of IGF1 and IGF2 mRNA in the developing cortex. IGF2 mRNA was highly expressed in the choroid plexus of E17 rat embryos, as well as in vascular endothelial cells and the leptomeninges of both E14 and E17 rat brain (FIGS. 14C and 14D). IGF1 and IGF2 mRNA was not detected levels (data not shown) in developing neural progenitor cells or the cortical mantle, as has been previously reported (Ayer-le Lievre et al., Development 111, 105-15 (1991)). While vascular sources of signaling molecules are important for neural progenitor cell fate (Shen et al. Science 304, 1338-40 (2004); Palmer et al., J Comp Neurol 425, 479-94 (2000)), our IGF2 expression data suggests that the choroid plexus is the primary source of IGF2 in the CSF.

To determine if CSF-borne IGF2 has the capacity to stimulate IGF signaling in the developing cortex, we first examined the localization of the IGF1 receptor (IGF1R) in the developing cortex. IGF1R, which binds IGF2 and is essential for the proliferative response to IGF signaling (references), localized to the apical, ventricular surface of radial neuroepithelial cells that contacts the CSF (FIG. 14E). Further, embryonic CSF activated IGF signaling in primary cortical precursor cells and neurons via the IGF1 receptor, as reflected by induction of phosphorylated IGF1Rβ (p-IGF1Rβ) (FIG. 14F). Embryonic CSF also stimulated the activation of the AKT and MAPK signaling pathways (FIG. 14F), both downstream targets of IGF signaling as well as other growth-factor-stimulated signaling cascades. IGF2 treatment alone induced IGF signaling similar to embryonic CSF (FIG. 14F). Thus, cortical progenitor cells appropriately express cell surface receptors required to engage CSF-borne cues such as IGF2, and reciprocally, CSF-borne IGF2 is capable of inducing the activation of IGF signaling in cortical progenitor cells (Hodge et al., J Neurosci 24, 10201-10 (2004); Dudek et al., Science 275, 661-5 (1997); Hodge et al., Int J Dev Neurosci 25, 233-41 (2007)).

We then tested whether Igf2 could maintain GLAST-positive cortical progenitor cells in vitro by culturing primary neurosphere dissociated cells with Igf2 (FIG. 15A). Interestingly, cells cultured in IGF2 formed small GLAST-positive-staining neurospheres (FIGS. 15A-15C) indicating that IGF2 alone provides a modest proliferative signal and that cells retain their neural progenitor cell fate in the presence of IGF2. We then determined whether IGF2 is both necessary and sufficient to induce maintenance and proliferation of neural progenitor cells along the ventricular zone in cortical explants. E16 cortical explants grown in E17 CSF control conditions showed numerous Vimentin 4A4-labeled, proliferating cells along the ventricle (FIG. 15E). In contrast, E16 explants cultured in E17 CSF and in the presence of an IGF2 neutralizing antibody (IGF2 NAb) revealed a striking decrease of Vimentin 4A4-labeled-cells along the ventricle (FIGS. 15E-15G) (E17 control mean: 28.8±4.3; E17 Igf2 neutralizing antibody mean: 13.9±2.0; n=4, p<0.05). In addition, addition of IGF2 (2 ng/ml) to Neural Basal Media (NBM) plus 20% ACSF stimulated the proliferation of Vimentin 4A4 positive progenitor cells in E16 explants (Igf2 supplementation mean: 36.7±2.1; control mean: 20.4±4.46; n=8, p<0.05) (FIGS. 15H-15J) and in E13 explants (data not shown).

Methods

The following methods were used to perform the above described experiments.

Cortical Explants

Rat embryos were removed from extra-embryonic membranes and placed in sterile Hanks Balanced Salt Solution (HBSS). The lateral wall of the developing cortex was dissected using a fine scalpel and demarcated in the rostral-caudal direction by the width of the lateral ganglionic eminence, in the dorsal direction by the in-fold of the medial cortical wall, and in the lateral direction by the border with the lateral ganglionic eminence. The dissected cortex was transferred to a polycarbonate membrane (Whatman; 13 mm, 8.0 um) using a platinum wire loop. Explants were then cultured for 24 hours in conditions described in text. Artificial CSF (ACSF) was made fresh for each use. ACSF consisted of NaCl 119 mM, KCl 2.5 mM, NaHCO₃26 mM, NaH₂PO₄1 mM, Glucose 11 mM, MgCl₂2 mM, CaCl₂2.8 mM. Supplemental IGF2 (US Biologicals) was added to ACSF at a final concentration of 2 ng/ml. 15 μg of IGF2 neutralization antibody in 15 μl of PBS (Millipore) was incubated with 100% E17 CSF for 1 hour rotating at 4° C. As a control, 15 μl of PBS was incubated with 100% E17 CSF. For BrdU labeling, explants were pulsed with BrdU for 30 minutes immediately prior to fixation. Explants were fixed (60% methanol, 30% chloroform, and 10% acetic acid) for 5-10 minutes, washed with 70% ethanol, embedded in paraffin, and sectioned at 5 um. Explant integrity was visualized by Hematoxylin and Eosin staining (data not shown).

Immunohistochemical and Immunoblot Analysis

The following antibodies were purchased: mouse anti-Tuj 11:250 (Covance), rat anti-BrdU 1:400 (AbD Serotec), rabbit anti-PH3 1:400 (Upstate), mouse anti-Vimentin 4A4 1:100 (Assay Designs), guinea pig anti-GLAST 1:100 (Company name), anti-phospho-Histone H3 1:400 (Upstate), rabbit P-AKT 1:100 (Cell Signaling), rabbit P-IGF1R, 1:100 (Cell Signaling), HRP conjugated anti-albumin 1:10,000 (Immunology Consultants Laboratory, Inc.), HRP conjugated anti-transferrin 1:1000 (Immunology Consultants Laboratory, Inc.), rabbit anti-Cystatin C 1:1000 (abeam), rabbit anti-Cathepsin B 1:1000 (abeam), rabbit anti-IGF2 1:100 (Santa Cruz Biotechnology), rabbit anti-FGF2 1:100 (Santa Cruz Biotechnology), rabbit anti-EC-SOD 1:1000 (Stressgen), mouse anti-APP 1:100 (Chemicon International).

Cortical Neurospheres

E14 rat cortex was dissected in sterile HBSS followed by gentle trituration. Primary spheres were generated in DMEM/F12 supplemented with heparin, N2, FGF (10 ng/ml), and EGF (20 ng/ml) and collected after 7-9 days in vitro (DIV). Primary spheres were then re-suspended in media without EGF or FGF, dissociated into single cells, plated at a final density of 2,500 cells/cm², and cultured in various media conditions. Fresh media was supplemented on day 4 of incubation. Cells were fixed in 4% Paraformaldehyde and stained for GLAST after 10 DIV.

Cortical Cell Cultures

Cultures of mixed cortical progenitor cells and neurons were prepared. Briefly, mouse embryonic E13.5 cortices were isolated and dissociated by Papain Dissociation System according to the manufacturer's instructions (Worthington Biochem. Corp). Cells were cultured in NBM supplemented with 1% penicillin-streptomycin, 1% glutamine, N2, and bFGF (10 ng/ml). The following day, cells were deprived of growth factors for 6 hours, followed by a 5 minute pulse of ACSF, embryonic CSF, or Igf2 (20 ng/ml).

In Situ Hybridization

Non-radioactive in situ hybridization was performed as described (Berger et al., J Comp Neurol 433, 101-14 (2001)), using a digoxigenin (DIG)-labelled cRNA probe generated from a TA vector (Invitrogen) clone of IGF1 or IGF2 cDNA and frozen rat brain sections.

Other Embodiments

All patents, patent applications, and publications mentioned in this specification, including U.S. Provisional Application No. 60/963,211, filed Aug. 3, 2007, are hereby incorporated by reference to the same extent as if each independent patent, patent application, or publication was specifically and individually indicated to be incorporated by reference.

EMBRYONIC CEREBROSPINAL FLUID (e-CSF), PROTEINS FROM e-CSF, AND RELATED METHODS AND COMPOSITIONS

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Publication Number

Date Filed

Date Published

Inventors

Original Assignees

CPC

US Classifications

International Classifications

Abstract

Description

Claims

STATEMENT AS TO FEDERALLY FUNDED RESEARCH

PCT Information

Provisional Applications (1)