Patent Grant
9770695
The present invention relates to a device and a preparation method for mixing a continuous phase and a dispersed phase with each other so as to form an emulsion.
As emulsion preparation devices in the field of medical equipment, chemical liquid preparation connectors described in Patent Documents 1 and 2 are reported. According to these connectors, a syringe charged with a continuous phase is linked to one side and a syringe charged with a dispersed phase is linked to the other side. Then, when pumping operation is performed alternately on both syringes, both phases are mixed with each other so that an emulsion is formed.
Nevertheless, in the connector of Patent Document 1, according to investigation by the present inventors, it has been recognized that: a large amount of pumping operation is necessary for forming a sufficient emulsion; further, depending on the composition of the chemical liquid, a sufficient emulsion cannot be formed in some cases; and further, an inconvenience of relatively high sliding resistance is present at the time of pumping operation. Further, the connector of Patent Document 1 does not include a filter part. A filter part in the connector of Patent Document 2 employs a porous material fabricated from a glass membrane. None of the connectors of both documents includes a filter part filled with fibers. Patent Document 3 relates to a filter filled with fibers but does not describe an emulsion preparation device. Further, Patent Document 4 also does not describe a connector provided with a filter part filled with fibers.
Patent Document 1: International Publication No. 2007/083763
Patent Document 2: Japanese Unexamined Patent Application Publication No. 2005-186026
Patent Document 3: Japanese Examined Patent Application Publication No. S52-35235
Patent Document 4: Japanese Unexamined Patent Application Publication No. 2006-346565
An object of the present invention is to provide an emulsion preparation device and an emulsion preparation method capable of forming an emulsion in a chemical liquid of diverse composition and further realizing a relatively low sliding resistance.
The present invention is characterized by an emulsion preparation device provided with a filter part, wherein: the filter part is constructed from a first and a second mesh part and fibers; and the fibers are loaded into a space between the first mesh part and the second mesh part. That is, the present invention provides the following (1) to (12).
(1) An emulsion preparation device provided with a filter part, wherein:
the filter part is constructed from a first and a second mesh part and fibers;
and the fibers are loaded into a space between the first mesh part and the second mesh part.
(2) The emulsion preparation device according to the above-mentioned (1), wherein:
one side or both sides of the filter part can be connected to a syringe; and
an emulsion is formed when a continuous phase and a dispersed phase perform, through the filter part, reciprocating movement between two syringes linked to the both sides of the filter part or alternatively between a syringe linked to one side and a vessel linked to the other side.
(3) The emulsion preparation device according to the above-mentioned (1) or (2), wherein
the first mesh part and/or the second mesh part are disks.
(4) The emulsion preparation device according to the above-mentioned (3), wherein
the mesh part includes a large number of through holes of arc shape arranged uniformly in a concentric manner and all the through holes have the same area as each other within an error range of 10%.
(5) The emulsion preparation device according to any one of the above-mentioned (1) to (4), wherein
the fibers are of a hydrophobic fiber.
(6) The emulsion preparation device according to the above-mentioned (5), wherein
the hydrophobic fiber is polyester.
(7) The emulsion preparation device according to any one of the above-mentioned (1) to (4), wherein
the fibers are of a hydrophilic fiber.
(8) The emulsion preparation device according to any one of the above-mentioned (1) to (7), wherein
the fibers have 50 to 150 deniers and are loaded such that 2.5 to 17.7 mm are present per 1 mm3 of the space.
(9) The emulsion preparation device according to any one of the above-mentioned (1) to (8), wherein
the fibers have 50 to 150 deniers and are loaded such that 5.0 to 9.9 mm are present per 1 mm3 of the space.
(10) The emulsion preparation device according to any one of the above-mentioned (1) to (9), constructed from
a first cylindrical member and a second cylindrical member, wherein:
the first cylindrical member is constructed from a first cylinder part and a second cylinder part continuous to the first cylinder part;
the second cylinder part has a smaller diameter than the first cylinder part;
in the first cylindrical member, the first mesh part is formed at a boundary between the first cylinder part and the second cylinder part, the fibers are pushed in toward the first mesh part, the second mesh part is pushed against the fibers, and, as a result, the filter part constructed from the first mesh part, the fiber aggregate, and the second mesh part is formed;
the second mesh part is a bottom face of a concave lid fit onto the first cylinder part;
in the concave lid, an outer flange in an aperture periphery abuts against an aperture periphery of the first cylinder part so that the second mesh part is positioned in the inside of the first cylinder part at a predetermined distance to the first mesh part and in parallel thereto; and
the first cylindrical member and the second cylindrical member are joined into a single piece by the outer flange in the aperture periphery of the first cylinder part and an outer flange in an aperture periphery of the second cylindrical member.
(11) The emulsion preparation device according to any one of the above-mentioned (1) to (9), constructed from
a first cylindrical member and a second cylindrical member, wherein:
the first cylindrical member is constructed from a first cylinder part and a second cylinder part continuous to the first cylinder part;
the second cylinder part has a smaller diameter than the first cylinder part;
in the first cylindrical member, the first mesh part is formed at a boundary between the first cylinder part and the second cylinder part, the fibers are pushed in toward the first mesh part, the second mesh part is pushed against the fibers, and, as a result, the filter part constructed from the first mesh part, the fiber aggregate, and the second mesh part is formed;
the second mesh part is a bottom face of a concave lid fit onto the first cylinder part;
in the concave lid, an outer flange in an aperture periphery abuts against an aperture periphery of the first cylinder part so that the second mesh part is positioned in the inside of the first cylinder part at a predetermined distance to the first mesh part and in parallel thereto;
the first cylindrical member and the second cylindrical member are joined into a single piece by the outer flange in the aperture periphery of the first cylinder part and an outer flange in an aperture periphery of the second cylindrical member;
the fiber aggregate is located in a center of a longitudinal direction; and
in a state that the first cylindrical member and the second cylindrical member have been joined into a single piece, an external shape is bilaterally symmetric in the longitudinal direction.
(12) An emulsion preparation method employing the emulsion preparation device according to any one of the above-mentioned (1) to (11).
The emulsion preparation method according to the present invention is characterized by employing the above-mentioned emulsion preparation device according to the present invention.
According to the present invention, in a chemical liquid of diverse composition, an emulsion can be formed and further the sliding resistance can be made relatively low.
The first cylindrical member 2 is constructed from a first cylinder part 21 and a second cylinder part 22 continuous to the first cylinder part 21. The second cylinder part 22 has a smaller diameter than the first cylinder part 21. In the first cylindrical member 2, a first mesh part 31 is formed at the boundary between the first cylinder part 21 and the second cylinder part 22.
Then, in the first cylindrical member 2, fibers 32 are pushed in toward the first mesh part 31 and the second mesh part 33 is pushed against the fibers 32. That is, the fibers 32 are pushed and loaded into a space 30 between the first mesh part 31 and the second mesh part 33. The first mesh part 31, the fibers 32, and the second mesh part 33 constitute a filter part 10. Here, the first mesh part 31 and the second mesh part 33 are disks provided with a large number of through holes. The fibers 32 loaded in the space 30 constitute a fiber aggregate filling the space 30. In the fiber aggregate, a large number of small voids are formed between the fibers. Thus, in the filter part 10, liquid can move back and forth from the first mesh part 31 to the second mesh part 33 and vice versa passing through the voids in the fiber aggregate.
The second mesh part 33 is the bottom face of a concave lid 23 fit onto the first cylinder part 21. In the concave lid 23, the outer flange 231 in the aperture periphery abuts against the aperture periphery 211 of the first cylinder part 21 so that the second mesh part 33 is positioned in the inside of the first cylinder part 21 at a predetermined distance to the first mesh part 31 and in parallel thereto.
A luer taper 48 is formed at the aperture end of the second cylindrical member 4. A luer taper 28 is formed also at the aperture end of the second cylinder part 22 of the first cylindrical member 2. The first cylindrical member 2 and the second cylindrical member 4 are in fluid communication with each other through apertures 20 and 40 of the same size as each other.
The fibers 32 are of a hydrophobic fiber. As the hydrophobic fiber, polyester, polypropylene, polystyrene, Teflon (registered trademark), nylon, polyvinyl chloride, acrylics, or the like may be employed. However, polyester is preferable. It is preferable that the fibers 32 are crimped. The fibers 32 have 50 to 150 deniers and are loaded into the space 30 such that 2.5 to 17.7 mm are present per 1 mm2 of the 2C space 30. Here, it is preferable that loading is performed such that 4.0 to 12.0 mm are present, and it is more preferable that loading is performed such that 5.0 to 9.9 mm are present.
The preparation instrument 100 shown in
First, the plunger of one syringe is pushed. For example, pumping operation in the direction A is performed on the plunger 82 of the syringe 8. By virtue of this, the dispersed phase 101 moves through the device 1 to the syringe 9 so that the plunger 92 of the syringe 9 is pushed aside in the direction A. At that time, in the syringe 9, the dispersed phase 101 is somewhat mixed with the continuous phase 102.
Next, pumping operation in the direction B is performed on the plunger 92 of the syringe 9. By virtue of this, the dispersed phase 101 and the continuous phase 102 somewhat mixed with each other move through the device 1 to the syringe 8 so that the plunger 82 of the syringe 8 is pushed aside in the direction B. At that time, in the device 1, both phases 101 and 102 somewhat mixed with each other pass through the filter part 10. That is, both phases 101 and 102 somewhat mixed with each other first pass through the second mesh part 33 so as to be dispersed and mixed at that time, then pass through the fibers 32 so as to be further dispersed and mixed at that time, and then pass through the first mesh part 31 so as to be further dispersed and mixed at that time. Thus, both phases 101 and 102 having moved to the syringe 8 are in a state of being mixed more than in the syringe 9.
Next, pumping operation in the direction A is performed on the plunger 82 of the syringe 8. By virtue of this, both phases 101 and 102 mixed more with each other move through the device 1 to the syringe 9 so that the plunger 92 of the syringe 9 is pushed aside in the direction A. At that time, in the device 1, both phases 101 and 102 mixed more with each other pass through the filter part 10. That is, both phases 101 and 102 mixed more with each other first pass through the first mesh part 31 so as to be dispersed and mixed at that time, then pass through the fibers 32 so as to be further dispersed and mixed at that time, and then pass through the second mesh part 33 so as to be further dispersed and mixed at that time. Thus, both phases 101 and 102 having moved to the syringe 9 are in a state of being mixed more than in the syringe 8.
As such, pumping operation on the plunger 82 of the syringe 8 and pumping operation on the plunger 92 of the syringe 9 are repeated alternately. It is preferable that the number of times of the pumping operation is 50 times or smaller. Further, ten times or smaller is more preferable and five times or smaller is the most preferable. By virtue of this, the state of mixing of both phases 101 and 102 progresses further into a state of emulsion which is a target state. Here, the fibers 32 are of a hydrophobic fiber. Thus, the oil phase serves as a continuous phase and the aqueous phase serves as a dispersed phase so that a water-in-oil type emulsion is formed.
According to the device 1 of the configuration, the fibers 32 have 50 to 150 deniers and are loaded into the space 30 such that 2.5 to 17.7 mm are present per 1 mm3 of the space 30. Thus, both phases 101 and 102 can be dispersed and mixed efficiently so that a desired emulsion can be formed.
Further, in the first mesh part 31 and the second mesh part 33, the through holes 311 of the same area as each other are arranged uniformly. Thus, dispersion of both phases 101 and 102 occurs uniformly in the entire region of the mesh part. Thus, also from this point, both phases 101 and 102 can be dispersed and mixed efficiently.
Further, the fibers 32 filling the space 30 have predetermined thickness and length. Further, the first mesh part 31 and the second mesh part 33 include a large number of the through holes 311 of arc shape and hence have a large void ratio. Thus, the sliding resistance at the time of pumping operation can be reduced. This improves the operability.
(i) The aggregate of the fibers 32 filling the space 30 is located in the center of the longitudinal direction.
(ii) The external shape is bilaterally symmetric in the longitudinal direction.
(iii) Liquid surface adjustment ribs 93 and 95 are provided.
That is, the difference is as follows.
The device 1A is constructed such that the first cylindrical member 2 and the second cylindrical member 4 are joined into a single piece by outer flanges 29 and 49 in the aperture periphery. Here, it is preferable that the device 1A is constructed from a sterilizable material.
The first cylindrical member 2 is constructed from a first cylinder part 21 and a second cylinder part 22 continuous to the first cylinder part 21. The second cylinder part 22 has a smaller diameter than the first cylinder part 21. In the first cylindrical member 2, a first mesh part 31 is formed at the boundary between the first cylinder part 21 and the second cylinder part 22.
Then, in the first cylindrical member 2, fibers 32 are pushed in toward the first mesh part. 31 and the second mesh part 33 is pushed against the fibers 32. That is, the fibers 32 are loaded into a space 30 between the first mesh part 31 and the second mesh part 33. The first mesh part 31, the fibers 32, and the second mesh part 33 constitute a filter part 10. Here, the first mesh part 31 and the second mesh part 33 are disks provided with a large number of through holes. The fibers 32 filling the space 30 constitute a fiber aggregate filling the space 30. In the fiber aggregate, a large number of small voids are formed between the fibers. Thus, in the filter part 10, liquid can move back and forth from the first mesh part 31 to the second mesh part 33 and vice versa passing through the voids in the fiber aggregate.
The second mesh part 33 is the bottom face of a concave lid 23 fit onto the first cylinder part 21. In the concave lid 23, the outer flange 231 in the aperture periphery abuts against the aperture periphery 211 of the first cylinder part 21 so that the second mesh part 33 is positioned in the inside of the first cylinder part 21 at a predetermined distance to the first mesh part 31 and in parallel thereto.
The first cylindrical member 2 and the second cylindrical member 4 are in fluid communication with each other through apertures 20 and 40 of the same size as each other.
Then, the aggregate of the fibers 32 filling the space 30 is located in the center of the longitudinal direction. That is, the space 30 is located in the center of the longitudinal direction.
Further, as seen From
The first mesh part 31, the fibers 32, and the second mesh part 33 are the same as those in the first embodiment.
When the preparation instrument 100 shown in
Further, in the device 1A, as shown in
Further, in the device 1A, the liquid surface adjustment ribs 93 and 95 indicate the upper limits for the height positions of the continuous phase and the dispersed phase at the time of air vent, and serve as guides used when the plungers 82 and 92 are pushed for air vent. Thus, according to the device 1A, the workability of air vent can be improved.
[Modified Structures]
The following modified structures may be adopted.
(1) The fibers 32 may be of a hydrophilic fiber. For example, cotton, rayon, vinylon, or the like may be employed. In this case, the aqueous phase serves as a continuous phase and the oil phase serves as a dispersed phase so that an oil-in-water type emulsion is formed.
(2) The first mesh part 31 and the second mesh part 33 may be disks as shown in
(3) The first mesh part 31 and the second mesh part 33 may have a shape other than the disk and, for example, may have the shape of a block.
(4) A mixed solution of a dispersed phase and a continuous phase may be loaded in any one of the syringe 8 and the syringe 9. In this case, no liquid is loaded in the other one.
The device 1 of examples 1 to 14 and the device 1A of example 15 were prepared. Then, emulsion check test A and sliding resistance evaluation test A were performed on the device 1 of examples 1 to 11. Further, emulsion check test B and sliding resistance evaluation test B were performed on the device 1 of examples 12, 13, and 14. Emulsion check test B was performed on the device 1A of example 15. Sliding resistance evaluation test C and foreign substance evaluation test were performed on the device 1 of example 12 and the device 1A of example 15.
The device 1 having the configuration of
The following point alone is different from example 1.
The following point alone is different from example 1.
The following point alone is different from example 1.
The following point alone is different from example 1.
The following points alone are different from example 1.
The following points alone are different from example 1.
The following points alone are different from example 1.
The following points alone are different from example 1.
The following points alone are different from example 1.
The following points alone are different from example 1.
The following points alone are different from example 1.
The following points alone are different from example 12.
The following points alone are different from example 12.
The device 1A having the configuration of
(Emulsion Check Test A)
[Test Method]
As shown in
(1) The preparation instrument 100 of
(2) Pumping operation was manually performed alternately on the plunger 82 of the syringe 8 and the plunger 92 of the syringe 9. This operation was repeated 5 times. As a result, both phases were accommodated into the syringe 8.
(3) The syringe 9 was removed. Then, as shown in
[Results]
Table 1 shows test results. Each test was performed three times.
When dripped liquid does not diffuse over the surface 521, an emulsion has been formed satisfactorily. This situation is indicated by “∘” in the test result. When dripped liquid diffuses over the surface 521, an emulsion has not been formed. This situation is indicated by “x” in the test result.
As seen from Table 1, in examples 1 to 11, a desired emulsion has been formed. In particular, in examples 1, 2, 3, 5, 6, 7, 9, and 10, a satisfactory emulsion has been formed.
(Emulsion Check Test B)
[Test Method]
As shown in
(1) The preparation instrument 100 of
(2) Pumping operation of alternately pushing on the plunger 82 of the syringe 8 and the plunger 92 of the syringe 9 was performed manually. This operation was repeated 5 times. As a result, both phases were accommodated into the syringe 8.
(3) The syringe 9 was removed. Then, as shown in
[Results]
Table 2 shows test results. Each test was performed twice.
The meanings of “∘” and “x” in the test results are the same as in emulsion check test A.
As seen from Table 2, even when the first mesh part 31 and the second mesh part 33 had whichever configuration of
(Sliding Resistance Evaluation Test A)
[Test Method]
As shown in
(1) The preparation instrument 100 of
(2) Pumping operation was manually performed alternately on the plunger 82 of the syringe 8 and the plunger 92 of the syringe 9. This operation was repeated 5 times. As a result, both phases were accommodated into the syringe 8.
(3) As shown in
Here, the sliding speed of the plungers 82 and 92 of both syringes 8 and 9 was set at 500 mm/min and 1000 mm/min.
[Results]
Table 3 shows test results. Each test was performed once for the sliding speed of the plunger 82 of 500 mm/min and performed twice for 1000 mm/min.
In a case that the pumping operation speed is 500 mm/min, the operability is light and satisfactory when the sliding resistance is lower than 70 N. Thus, this situation is indicated by “∘”. Further, in case of 70 N or higher, this situation is indicated by “x”. Further, in a case that the pumping operation speed is 1000 mm/min, the operability is light and satisfactory when the sliding resistance is lower than 140 N. Thus, this situation is indicated by “∘”. Further, in case of 140 N or higher, this situation is indicated by “x”.
As seen from Table 3, in examples 1 to 11, the operability of pumping was satisfactory.
(Sliding Resistance Evaluation Test B)
[Test Method]
As shown in
(1) The preparation instrument 100 of
(2) Pumping operation was manually performed alternately on the plunger 82 of the syringe 8 and the plunger 92 of the syringe 9. This operation was repeated 5 times. As a result, both phases were accommodated into the syringe 8.
(3) As shown in
[Results]
As seen from Table 4, in examples 12, 13, and 14, the sliding resistance was lower than the conventional art. Thus, the operability was satisfactory. In particular, in example 12, that is, in a case that the mesh part having the configuration of
(Sliding Resistance Evaluation Test C)
[Test Method]
As shown in
(1) The preparation instrument 100 of
(2) Pumping operation was manually performed alternately on the plunger 82 of the syringe 8 and the plunger 92 of the syringe 9. This operation was repeated 5 times. As a result, both phases were accommodated into the syringe 8.
(3) As shown in
[Results]
Table 5 shows test results.
As seen from Table 5, in examples 12 and 15, the sliding resistance was lower than the conventional art. Thus, the operability was satisfactory.
(Foreign Substance Evaluation Test)
[Test Method]
(1)
(2) Japanese Pharmacopoeia Sixteenth Edition “Insoluble Particulate Matter Test for Injections, Method 1. Light Obscuration Particle Count Test” was performed on the sample. Specifically, insoluble particulates per 10 ml of the sample were measured four times with an in-liquid particulate measurement instrument (product name: RION KL-04). Then, the second to the fourth measurement values were converted into the number of particulates per vessel. This measurement was performed five times in total with changing the sample.
[Results]
Table 6 shows the results of example 12. Table 7 shows the results of example 15.
With reference to a test method “B. Solutions for injection supplied in containers with a nominal content of less than 100 ml” in the above-mentioned Japanese Pharmacopoeia, the allowance criterion for the average number of particulates is “6000 or fewer for particulates of 10 μm or larger and 600 or fewer for particulates of 25 μm or larger, per vessel”. However, in the present test, a ten-fold severer allowance criterion was employed that “600 or fewer for particulates of 10 μm or larger and 60 or fewer for particulates of 25 μm or larger, per vessel”.
In both of examples 12 and 15, the severer allowance criterion has been satisfied. Thus, both devices 1 and 1A are excellent in the foreign substance quality and hence have sufficient cleanliness for the use as medical equipment.
The emulsion preparation device of the present invention can form an emulsion for a chemical liquid of diverse composition, further can realize a relatively low sliding resistance, and hence has a great advantage in industrial utilization.
1: 1A Device, 10: Filter part, 100: Preparation instrument, 2: First cylindrical member, 21: First cylinder part, 211: Aperture periphery, 22: Second cylinder cart, 23: Concave lid, 231: Outer flange, 28: Luer taper, 31: First mesh part, 311: Through hole, 32: Fibers, 33: Second mesh part, 4: Second cylindrical member, 29, 49: Outer flange, 8, 9: Syringe
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| WO2013/133209 | 9/12/2013 | WO | A |
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