Patent Application
20140011818
The present invention relates to enantiomers of fluconazole analogues containing thieno-[2,3-d]pyrimidin-4(3H)-one moiety as antifungal agents, which are depicted by Formula (1a) and Formula (1b), and pharmaceutically acceptable salts thereof,
Wherein R1 is hydrogen or a halogen selected from fluorine, chlorine, bromine or iodine; R2 is hydrogen or a halogen selected from fluorine, chlorine, bromine or iodine; and R3 and R4 may be the same or different, and each represents a hydrogen, alkyl group of linear or branched chain of 1 to 20 carbon atoms optionally substituted with aryl group, hydroxyl group, alkanoate group, acetoxy group, amino acetoxy group, N-Boc-amino acetoxy group, alkoxy (—OR) group (wherein R=alkyl group with 1 to 4 carbon atoms), benzyloxy, arylalkyl group (wherein the aryl group is phenyl optionally substituted with alkyl group of 1 to 3 carbon atoms); or R3 and R4 together form a cycloalkyl ring of 3 to 10 carbon atoms.
The invention further relates to a process for preparation of the enantiomers of fluconazole analogues containing thieno-[2,3-d]pyrimidin-4(3H)-one moiety of Formula (1a) and Formula (1b), and pharmaceutical preparations containing these compounds for prevention and treatment of fungal infections.
Fungus is a type of microorganism that causes fungal infection. A fungal infection is an inflammatory condition in which fungi multiply and invade the skin, the digestive tract, the genitals and other body tissues, particularly the lungs and liver. Fungal infections mainly include superficial and systemic fungal infections. Fungal infections are more common in people taking antibiotics, corticosteroids, immunosuppressant drugs and contraceptives. The fungal infections are prominent in people with endocrine disorders, immune diseases and other conditions such as obesity, AIDS, tuberculosis, major burns, leukemia and diabetes.
The current antifungal agents belong to various groups like polyenes, heterocyclic benzofuran, allylamines, antimetabolites, azoles, glucan synthesis inhibitors, etc. out of which azoles are presently the most extensively used antifungal agents. Azoles are further classified into imidazoles and triazoles. Fluconazole belongs to the family of triazole antifungals. Fluconazole is an important antifungal agent which is orally active and has low toxicity but its extensive use has resulted in emergence of fluconazole-resistant fungal strains. Therefore, it is necessary to meet the long-felt need to develop novel fluconazole analogues which exert high anti-fungal activity against various fungi. The presence of one triazole ring, halogenated phenyl ring and tertiary alcoholic oxygen functionality in azole class of compounds, is necessary for antifungal activity.
Various fluconazole analogues having antifungal activity have been reported in the literature. Some of the recent references describing synthesis and antifungal activity are given below:
Bioorganic & Medicinal Chemistry Letters 17 (2007) 3686-9; Bioorganic & Medicinal Chemistry Letters 18 (2008) 6538-6541; Bioorganic & Medicinal Chemistry Letters 19 (2009) 301-304; Bioorganic & Medicinal Chemistry Letters 19 (2009) 759-763; Bioorganic & Medicinal Chemistry Letters 19 (2009) 2013-2017; and Bioorganic & Medicinal Chemistry Letters 19 (2009) 3559-3563.
The racemic fluconazole analogues containing thieno-[2,3-d]pyrimidin-4(3H)-one moiety of Formula (2) and their excellent antifungal activities have already been described in our earlier patent publication, WO 2009109983, with the method of preparing such racemic compounds, which have high antifungal activity against various fungi.
Wherein, R1, R2, R3 and R4 are defined as above.
It has been found by the present inventors that one of the enantiomer of chiral fluconazole analogues containing thieno-[2,3-d]pyrimidin-4(3H)-one moiety has enhanced antifungal activity than corresponding racemic compounds. Hence, there is a need to develop such enantiomers which exert high antifungal activity against various fungal strains.
Accordingly, the present invention seeks to provide enantiomers of chiral fluconazole analogues of Formula (1a) and Formula (1b) containing thieno-[2,3-d]pyrimidin-4(3H)-one moiety and process thereof as an effort to come up with antifungal agents having broad spectrum of antifungal activity, for which the protection is sought.
The primary objective of the present invention is to provide compounds of Formula (1a) and Formula (1b) containing thieno-[2,3-d]pyrimidin-4(3H)-one moieties with enhanced antifungal activity against various fungal strains.
Another objective of the present invention is to provide a process for the preparation of compounds of Formula (1a) and Formula (1b) containing thieno-[2,3-d]pyrimidin-4(3H)-one moieties having enhanced antifugal activity.
Accordingly, to meet the above stated objectives, the present invention discloses enantiomers of fluconazole analogues containing thieno-[2,3-d]pyrimidin-4(3H)-one moiety as antifungal agents, which are depicted by Formula (1a) and Formula (1b).
Wherein R1 is hydrogen or a halogen selected from fluorine, chlorine, bromine or iodine; R2 is hydrogen or a halogen selected from fluorine, chlorine, bromine or iodine; and R3 and R4 may be the same or different, and each represents a hydrogen, alkyl group of linear or branched chain of 1 to 20 carbon atoms optionally substituted with aryl group, hydroxyl group, alkanoate group, acetoxy group, amino acetoxy group, N-Boc-amino acetoxy group, alkoxy (—OR) group (wherein R=alkyl group with 1 to 4 carbon atoms), benzyloxy, arylalkyl group (wherein the aryl group is phenyl optionally substituted with alkyl group of 1 to 3 carbon atoms; or R3 and R4 together form a cycloalkyl ring of 3 to 10 carbon atoms. The invention encompasses the pharmaceutical salts of Formula (1a) and (1b).
The invention further discloses a process for preparation of compounds of Formula (1a) and Formula (1b), and pharmaceutical preparations containing these compounds, for prevention and treatment of fungal infections. Such chiral, optically-active compounds acting as antifungals, have MIC values much smaller than that of racemic compounds of Formula (2) as well as fluconazole.
According to the present invention, there are provided pure enantiomers of fluconazole analogues containing thieno-[2,3-d]pyrimidin-4(3H)-one moiety, as depicted in Formula (1a) and Formula (1b). These compounds belong to azole class of antifungal compounds and are analogues of fluconazole, which is active against fungi; and are to be used in pharmaceutical preparations as active agent.
Wherein R1 is hydrogen or a halogen selected from fluorine, chlorine, bromine or iodine; R2 is hydrogen or a halogen selected from fluorine, chlorine, bromine or iodine; and R3 and R4 may be the same or different, and each represents a hydrogen, alkyl group of linear or branched chain of 1 to 20 carbon atoms optionally substituted with aryl group, hydroxyl group, alkanoate group, acetoxy group, amino acetoxy group, N-Boc-amino acetoxy group, alkoxy (—OR) group (wherein R=alkyl group with 1 to 4 carbon atoms), benzyloxy, arylalkyl group (wherein the aryl group is phenyl optionally substituted with alkyl group of 1 to 3 carbon atoms); or R3 and R4 together form a cycloalkyl ring of 3 to 10 carbon atoms.
The invention further relates to the process for preparation of the compounds of Formula (1a) and Formula (1b). The compounds of Formula (1a) and Formula (1b) of the present invention are prepared either by a synthetic process as illustrated in Scheme 1, or by chiral separation using HPLC (High Performance Liquid Chromatography) as illustrated in Scheme 2.
Wherein, R1, R2, R3 and R4 are defined as above, and “*” is used to designate R or S configuration at carbon atom.
Accordingly, the process for the preparation of compound of Formula (1a) and Formula (1b) comprises reacting a compound of Formula (3) with a chiral epoxide of Formula (4), in presence of a suitable base and a catalyst. The suitable base used in the present invention is selected from various organic or inorganic bases preferably inorganic base such as potassium carbonate, sodium carbonate or cesium carbonate. The suitable catalyst used in the present invention is selected from various phase transfer catalysts such as tetrabutylammonium bromide, trtrabutylammonium chloride, triethylbenzylammonium chloride or cetyltrimethylammonium bromide.
Wherein, R1, R2, R3 and R4 are defined as above.
Compounds of Formula (1a) and Formula (1b) can also be prepared by chiral separation of racemic compounds of Formula (2) using chiral HPLC in order to obtain desired enantiomer, as shown in Scheme 2. The chiral HPLC is performed using a chiral preparative HPLC column and a mobile phase. Compounds of Formula (2) can be prepared as per the method disclosed in WO 2009109983.
According to the present invention, the chiral preparative HPLC column is selected from but not limited to cellulose tris (3,5-dimethylphenylcarbamate) coated on silica-gel, cellulose tris (4-methylbenzoate) coated on silica-gel or tris-(3,5-dimethylphenyl)-carbamoyl amylose coated on silica-gel; and the eluent system is isocratic system comprising a mixture of hydrocarbon(s), alcohol(s) and/or alkylamine(s).
The hydrocarbons used in the present invention are selected from a group consisting of pentane, hexane, heptane, iso-octane, cyclohexane and cyclopentane.
The alcohol used in the present invention is selected from the group consisting of methanol, ethanol, propan-1-ol, propan-2-ol, butan-1-ol, butan-2-ol, tert-butanol, 3-methyl-1-butanol, 2-methyl-1-propanol, 2-methoxyethanol and 2-ethoxyethanol.
The alkylamine used in the present invention is selected from a group consisting of diethylamine, triethylamine, isopropylamine and butylamine.
The ratio of alcohol in the mobile phase of the eluent system ranges from 10% to 60%. The ratio of hydrocarbon in the mobile phase of the eluent system ranges from 40% to 90%. The ratio of alkylamine in the mobile phase of the eluent system ranges from 0% to 2%.
The present invention further discloses a pharmaceutical preparation for treating or preventing fungal infections, comprising compounds of Formula (1a) or Formula (1b), in association with at least one pharmaceutically acceptable excipient known in the art. These excipients are added to the composition for variety of purposes.
The pharmaceutical preparations according to the invention may be administered as oral, topical and parenteral dosage forms.
The pharmaceutical preparations can be selected from various oral dosage forms such as solid dosage form including tablets, capsules, pellets, powders, soft gelatin capsules and oral liquids. The pharmaceutical compositions can be prepared using conventional techniques well known in the art.
The invention provides a method for treating or preventing fungal infections in a subject, wherein the said method comprises administering to the subject, therapeutically effective amounts of the compounds of Formula (1a) or Formula (1b) of the present invention. The subject according to the invention is a mammal. The compounds of the present invention can also be administered optionally with other actives depending on the disease conditions.
The term “therapeutically effective amount” as used herein, means an amount used in the pharmaceutical preparations to achieve the desired therapeutic effect.
The amount/quantity of the compound used in pharmaceutical compositions of the present invention will vary depending upon the body weight of the patient, and the mode of administration and can be of any effective amount to achieve the desired therapeutic effect.
The present invention further provides use of the compound of Formula (1a) or (1b) in the treatment and prevention of variety of fungal diseases. The active compounds of Formula (1a) or (1b) are effective against variety of fungal species.
The present invention also provides the use of compound of Formula (1a) or (1b) in the preparation of pharmaceutical medicament for the treatment and prevention of variety of fungal diseases.
The pharmaceutical medicament according to the invention may be administered as oral, topical and parenteral dosage forms.
The invention is further illustrated with the following examples and should not be construed to limit the scope of the present invention. The features of the present invention will become more apparent from the following description of the inventive concept and the description of the preferred embodiments.
Some of the compounds of Formula 1a, 1b, 2, 3 and 4 are illustrated in Tables 1,2,3,4 and 5 respectively.
Wherein, R1, R2, R3 and R4 are defined as above, and “*” is used to designate R or S configuration at carbon atom.
Compound of Formula 3 (1 equivalent) was taken in two necked round bottom flask equipped with reflux condenser and guard tube. Organic solvent such as ethyl acetate was added followed by the addition of flame-dried base (0.5-4 equivalents) and catalyst (0.1-2 equivalent). The mixture was stirred at room temperature for 0.5-4 h and compound of Formula 4a (1 equivalent) in ethyl acetate was added. The mixture was stirred under reflux for 5-18 h, cooled, diluted with water, extracted with ethyl acetate, dried over Na2SO4 and concentrated. Purification by recrystallization from ethyl acetate afforded the pure product.
6-(n-Hexyl)thieno[2,3-d]pyrimidin-4(3H)-one (Formula 3-01) (0.99 g, 4.21 mmol) was taken in two necked round bottom flask equipped with reflux condenser and guard tube. Ethyl acetate (50 ml) was added followed by the addition of flame dried potassium carbonate (1.16 g, 8.43 mmol) and tetrabutylammonium bromide (1.36 g, 4.21 mmol). The mixture was stirred at room temperature for 1 h and (R)-1-[2-(2,4-difluorophenyl)-oxiranylmethyl]-1H-[1,2,4]triazole (Formula 4b) (1.0 g, 4.21 mmol) in ethyl acetate (20 ml) was added. The mixture was stirred under reflux for 12 h, cooled, diluted with water (100 ml), extracted with ethyl acetate (3×40 ml), dried over Na2SO4 and concentrated. Purification on column chromatography afforded the pure product as pale brown solid (1.51 g, 75.7%).
Nature: Pale brown solid; MP: 90° C.; Yield: 75.7%; 1H NMR (200 MHz, CDCl3): δ 0.88 (t, J=6 Hz, 3H), 1.21-1.48 (m, 6H), 1.61-1.76 (m, 2H), 2.82 (t, J=8 Hz, 2H), 4.22 (d, J=14 Hz, 1H), 4.52 (d, J=14 Hz, 1H), 4.72 (d, J=14 Hz, 1H), 4.80 (d, J=14 Hz, 1H), 6.24 (s, 1H), 6.75-6.89 (m, 2H), 7.07 (s, 1H), 7.48-7.62 (m, 1H), 7.83 (s, 1H), 7.91 (s, 1H), 8.10 (s, 1H); [α]D25=+96° (c=1.0, Chloroform).
6-(n-Pentyl)thieno[2,3-d]pyrimidin-4(3H)-one (Formula 3-02) (4.6 g, 0.021 mol) was taken in two necked round bottom flask equipped with reflux condenser and guard tube. Ethyl acetate (250 ml) was added followed by the addition of flame dried potassium carbonate (5.72 g, 0.042 mol) and tetrabutylammonium bromide (6.693 g, 0.021 mol). The mixture was stirred at room temperature for 30 min and (R)-1-[2-(2,4-difluorophenyl)-oxiranylmethyl]-1H-[1,2,4]triazole (Formula 4b) (4.91 g, 0.021 mol) in ethyl acetate (50 ml) was added. The mixture was stirred under reflux for 14 h, cooled, diluted with water (250 ml), extracted with ethyl acetate (3×150 ml), dried over Na2SO4 and concentrated. Purification by recrystallization from ethyl acetate afforded the pure product as pale yellow solid (7.11 g, 73.8%).
Nature: Pale yellow solid; MP: 152° C.; Yield: 73.8%; 1H NMR (200 MHz, CDCl3): δ 0.90 (t, J=6 Hz, 3H), 1.27-1.42 (m, 4H), 1.64-1.76 (m, 2H), 2.82 (t, J=8 Hz, 2H), 4.21 (d, J=15 Hz, 1H), 4.52 (d, J=15 Hz, 1H), 4.71 (d, J=15 Hz, 1H), 4.78 (d, J=15 Hz, 1H), 6.24 (s, 1H), 6.76-6.88 (On, 2H), 7.06 (s, 1H), 7.50-7.64 (m, 1H), 7.81 (s, 1H), 7.90 (s, 1H), 8.09 (s, 1H); [α]D25=+98° (c=1.0, Chloroform).
6-(n-Heptyl)thieno[2,3-d]pyrimidin-4(3H)-one (Formula 3-03) (0.502 g, 0.0021 mol) was taken in two necked round bottom flask equipped with reflux condenser and guard tube. Ethyl acetate (10 nil) was added followed by the addition of flame dried potassium carbonate (0.582 g, 0.0042 mol) and tetrabutylammonium bromide (0.681 g, 0.0021 mol). The mixture was stirred at room temperature for 1 h and (R)-1-[2-(2,4-difluorophenyl)-oxiranylmethyl]-1H-[1,2,4]triazole (Formula 4b) (0.50 g, 0.0021 mol) in ethyl acetate (5 ml) was added. The mixture was stirred under reflux for 12 h, cooled, diluted with water (50 ml), extracted with ethyl acetate (3×20 ml), dried over Na2SO4 and concentrated. Purification by column chromatography afforded the pure product as cream white solid (0.834 g, 81.2%).
Nature: Cream white solid; MP: 98° C.; Yield: 81.2%; 1H NMR (200 MHz, CDCl3): δ 0.86 (t, J=6 Hz, 3H), 1.21-1.32 (m, 8H), 1.62-1.72 (m, 2H), 2.81 (t, J=8 Hz, 2H), 4.23 (d, J=15 Hz, 1H), 4.53 (d, J=15 Hz, 1H), 4.72 (d, J=15 Hz, 1H), 4.80 (d, J=15 Hz, 1H), 6.24 (bs, 1H), 6.74-6.89 (m, 2H), 7.06 (s, 1H), 7.48-7.60 (m, 1H), 7.84 (s, 114), 7.91 (s, 1H), 8.13 (s, 1H); [α]D25=+110° (c=1.0, Chloroform).
6-(n-Propyl)thieno[2,3-d]pyrimidin-4(3H)-one (Formula 3-04) (0.82 g, 0.0042 mol) was taken in two necked round bottom flask equipped with reflux condenser and guard tube. Ethyl acetate (15 ml) was added followed by the addition of flame dried potassium carbonate (1.16 g, 0.0084 mol) and tetrabutylammonium bromide (1.36 g, 0.0042 mol). The mixture was stirred at room temperature for 0.5 h and (R)-1-[2-(2,4-difluorophenyl)-oxiranylmethyl]-1H-[1,2,4]triazole (Formula 4b) (1.00 g, 0.0042 mol) in ethyl acetate (10 ml) was added. The mixture was stirred under reflux for 12 h, cooled, diluted with water (50 ml), extracted with ethyl acetate (4×25 ml), dried over Na2SO4 and concentrated. Purification on column chromatography afforded the pure product as off-white solid (1.36 g, 74.6%).
Nature: Off-white solid; MP: 94° C.; Yield: 74.6%; 1H NMR (200 MHz, CDCl3): δ 0.99 (t, J=7 Hz, 3H), 1.61-1.83 (m, 2H), 2.80 (t, J=8 Hz, 2H), 4.27 (d, J=14 Hz, 1H), 4.59 (d, J=14 Hz, 1H), 4.73 (d, J=14 Hz, 1H), 4.84 (d, J=14 Hz, 1H), 6.28 (bs, 1H), 6.74-6.90 (m, 2H), 7.08 (s, 1H), 7.46-7.61 (m, 1H), 7.88 (s, 1H), 7.92 (s, 1H), 8.28 (s, 1H); [α]D25=+92° (c=1.0, Chloroform).
6-(n-Pentyl)thieno[2,3-d]pyrimidin-4(3H)-one (Formula 3-02) (1.00 g, 4.21 mmol) was taken in two necked round bottom flask equipped with reflux condenser and guard tube. Ethyl acetate (30 ml) was added followed by the addition of flame dried potassium carbonate (1.16 g, 8.43 mmol) and tetrabutylammonium bromide (1.36 g, 4.21 mmol). The mixture was stirred at room temperature for 1 h and (S)-1-[2-(2,4-difluorophenyl)-oxiranylmethyl]-1H-[1,2,4]triazole (Formula 4a) (1.0 g, 4.21 mmol) in ethyl acetate (50 ml) was added. The mixture was stirred under reflux for 12 h, cooled, diluted with water (100 ml), extracted with ethyl acetate (3×40 ml), dried over Na2SO4 and concentrated. Purification by recrystallization from ethyl acetate afforded the pure product as pale yellow solid (1.44 g, 74.6%).
Nature: Pale yellow solid; MP: 153° C.; Yield: 73.8%; 1H NMR (200 MHz, CDCl3): δ 0.90 (t, J=6 Hz, 3H), 1.27-1.42 (m, 4H), 1.64-1.76 (m, 2H), 2.82 (t, J=8 Hz, 2H), 4.21 (d, J=15 Hz, 1H), 4.52 (d, J=15 Hz, 1H), 4.71 (d, J=15 Hz, 1H), 4.78 (d, J=15 Hz, 1H), 6.24 (s, 1H), 6.76-6.88 (m, 2H), 7.06 (s, 1H), 7.50-7.64 (m, 1H), 7.81 (s, 1H), 7.90 (s, 1H), 8.09 (s, 1H); [α]D25=−99° (c=1.0, Chloroform).
Compounds of Formula 2 in Scheme 2 were prepared as described in WO 2009109983. The racemic compounds of Formula 2 were first analyzed by analytical HPLC on chiral column to get separation and developed the conditions for preparative chiral HPLC in order to isolate the enantiomers in pure form. Thus, the racemic compounds of Formula 2 were separated into their R and S enantiomers of Formula (1a) and Formula (1b) respectively, using chiral preparative HPLC. The analytical as well as chiral preparative HPLC was carried out under following general conditions:
The racemic compounds of Formula 2 were separated into their R and S enantiomers of Formula (1a) and Formula (1b) respectively using preparative HPLC under following conditions, and the retention time for various compounds are shown in the following table 6:
The racemic compounds of Formula 2 were separated into their R and S enantiomers of Formula (1a) and Formula (1b) respectively using preparative HPLC under following conditions and the retention time for various compounds are shown in the following table 7:
The racemic compounds of Formula 2 were separated into their R and S enantiomers of Formula (1a) and Formula (1b) respectively using preparative HPLC under following conditions and the retention time for various compounds are shown in the following table 8:
After carrying out the chiral preparative HPLC, the resulting products were analysed by chiral analytical HPLC for ascertaining optical purity. The optical rotations for various compounds are shown in the following table 9.
The compounds of Formula (1a) and (1b) were tested for antifungal activity against various strains of Candida(CA 01: Candida albicans ATCC 24433, CA 02: C. albicans ATCC 10231, CA 03: C. albicans ATCC 2091, CA 04: C. albicans ATCC 90028, CG 01: C. glabrata ATCC 90030, CK 01: C. Krusei ATCC 6258, CT 01: C. tropicalis ATCC 750). In vitro evaluation of antifungal activity was performed by determining the minimum inhibitory concentration (MIC) following standard broth dilution methods (CLSI: Reference method for broth dilution antifungal susceptibility testing of yeasts; Approved standard, second edition M27-A2, 2002; CLSI: Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; Approved standard M38-A, 2002) with RPMI 1640 medium buffered to pH 7.0 with MOPS buffer. Known anti-fungal agents like Fluconazole and Amphotericin-B were used as standards. End points were determined after 48 hours visually and by using spectrophotometer wherever necessary. The activity parameters are enumerated in Table 10:
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative examples and that the present invention may be embodied in other specific forms without departing from the essential attributes thereof, and it is therefore desired that the present embodiments and examples be considered in all respects as illustrative and not restrictive.
| Number | Date | Country | Kind |
|---|---|---|---|
| 735/MUM/2011 | Mar 2011 | IN | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/IN2011/000371 | 5/31/2011 | WO | 00 | 9/12/2013 |
