ENHANCED ROLLER BOTTLE FOR CELL CULTURE

Information

  • Research Project
  • 2792660
  • ApplicationId
    2792660
  • Core Project Number
    R43GM058978
  • Full Project Number
    1R43GM058978-01
  • Serial Number
    58978
  • FOA Number
  • Sub Project Id
  • Project Start Date
    2/1/1999 - 25 years ago
  • Project End Date
    1/31/2000 - 24 years ago
  • Program Officer Name
    TYREE, BERNADETTE
  • Budget Start Date
    2/1/1999 - 25 years ago
  • Budget End Date
    1/31/2000 - 24 years ago
  • Fiscal Year
    1999
  • Support Year
    1
  • Suffix
  • Award Notice Date
    1/25/1999 - 25 years ago

ENHANCED ROLLER BOTTLE FOR CELL CULTURE

An enhanced roller bottle concept is proposed. A roller bottle with an 8 fold or greater increase in bottle capacity will significantly aid operations in which multiple cultures are maintained. Importantly, tissue engineering is experiencing rapid growth. For many applications, one rate limiting step is availability of tissue and the need to expand small samples to clinically relevant endpoints. A compact, high capacity cell culture device with direct oxygenation (enhanced roller bottle) will be valuable. In autologous cultures, a compact, high capacity device that is modular, and efficient is needed. The device will be single use and simple to operate. Feasibility testing of the critical features required for such a device is to be done. The gas exchange/growth surface will be proven out. Multiple approaches will demonstrate robustness of the concept. The most suitable candidate will be incorporated into scale prototypes for device design prove out. Cell inoculation, capacity and recovery following culture will determine feasibility of the existing concept. Evaporative loss and medium foaming will be evaluated. If required, alternative designs will be tested. Final revised prototypes will be tested in the expansion of chondrocytes by an independent laboratory involved in a clinical trial using expanded chondrocytes. PROPOSED COMMERCIAL APPLICATION Annual use of roller bottles for cell culture is estimated to be between 25-40 x 106 bottles per year in the United States alone. The enhanced roller bottle is intended to be equivalent to at least 8 existing bottles with regards to the capacity of cells that can be supported. The enhanced roller bottle is envisioned to be an ideal device for tissue engineering as well as numerous other cell culture applications.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    R43
  • Administering IC
    GM
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    821
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    ZRG2
  • Study Section Name
  • Organization Name
    WILSON WOLF MANUFACTURING CORPORATION
  • Organization Department
  • Organization DUNS
  • Organization City
    NEW BRIGHTON
  • Organization State
    MN
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    55112
  • Organization District
    UNITED STATES