Claims
- 1. An improved tissue graft construct comprising
vertebrate submucosa delaminated from both the external smooth muscle layers and the luminal portions, of the tunica mucosa and added primary epithelial cells.
- 2. The improved tissue graft construct of claim 1 wherein the vertebrate submucosa comprises tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of vertebrate intestinal tissue.
- 3. The improved tissue graft construct of claim 1 wherein the primary epithelial cells are selected from the group consisting of primary gingiva epithelial cells and primary esophageal epithelial cells.
- 4. The improved tissue graft construct of claim 2, wherein the submucosa is fluidized submucosa.
- 5. The improved tissue graft construct of claim 4, wherein the fluidized submucosa comprises submucosa digested with an enzyme for a period of time sufficient to solubilize the submucosa.
- 6. A composition for the repair of articular cartilage defects, said composition comprising
tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of vertebrate intestinal tissue and added primary chondrocyte cells.
- 7. The composition of claim 6, wherein the submucosal tissue is fluidized submucosal tissue.
- 8. The composition of claim 7, wherein the fluidized submucosal tissue comprises submucosal tissue digested with an enzyme for a period of time sufficient to solubilize the tissue.
- 9. A method for enhancing the capabilities of a vertebrate submucosa graft construct to repair articular cartilage defects, said method comprising the step of seeding the vertebrate submucosa graft construct with chondrocytes prior to implanting or injecting the vertebrate submucosa graft construct into a host.
- 10. The method of claim 9 further comprising the step of subjecting the seeded vertebrate submucosa graft construct to conditions conducive to the proliferation of the cells prior to implanting or injecting the vertebrate submucosa graft material into the host.
- 11. The method of claim 10 wherein the vertebrate submucosa comprises tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of vertebrate intestinal tissue.
- 12. A method for enhancing the capabilities of a vertebrate submucosa graft construct to repair periodontal defects, said method comprising the step of seeding the vertebrate submucosa graft construct with primary gingiva epithelial cells prior to implanting or injecting the vertebrate submucosa graft construct into a host.
- 13. The method of claim 12 further comprising the step of subjecting the seeded vertebrate submucosa graft construct to conditions conducive to the proliferation of the cells prior to implanting or injecting the vertebrate submucosa graft material into the host.
- 14. The method of claim 12 wherein the vertebrate submucosa comprises tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of vertebrate intestinal tissue.
- 15. An improved tissue graft construct comprising
vertebrate submucosa delaminated from both the external smooth muscle layers and the luminal portions of the tunica mucosa and added primary cells.
- 16. The improved tissue graft construct of claim 15 wherein the vertebrate submucosa comprises tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of vertebrate intestinal tissue.
- 17. The improved tissue graft construct of claim 15 wherein the primary cells are selected from the group consisting of endothelial cells, keratinocytes, chondrocytes, epithelial cells and mesenchymal cells.
- 18. The improved tissue graft construct of claim 16 wherein the submucosa is fluidized submucosa.
- 19. The improved tissue graft construct of claim 18 wherein the fluidized submucosa comprises submucosa digested with an enzyme for a period of time sufficient to solubilize the submucosa.
- 20. An improved tissue graft construct comprising
tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of vertebrate intestinal tissue, and a population of cells selected from the group consisting of endothelial cells, keratinocytes and mesenchymal cells.
- 21. The improved tissue graft construct of claim 20 wherein the preselected cell type comprises cells that have been genetically modified.
- 22. The improved tissue graft construct of claim 20 wherein the submucosa tissue is fluidized submucosal tissue.
- 23. The improved tissue graft construct of claim 21 wherein the fluidized submucosal tissue comprises submucosal tissue digested with an enzyme for a period of time sufficient to solubilize the tissue.
- 24. A method for enhancing the capabilities of a submucosa graft construct to repair epithelial defects, said method comprising the step of seeding the submucosa with primary epithelial cells prior to implanting or injecting the graft construct into a host.
- 25. The method of claim 24 further comprising the step of subjecting the seeded graft construct to conditions conducive to the proliferation of the cells prior to injecting the graft material into the host.
- 26. The method of claim 24 wherein the submucosa comprises tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of vertebrate intestinal tissue.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation of U.S. application Ser. No. 09/151,790, filed Sep. 11, 1998, which is a continuation-in-part of U.S. application Ser. No. 08/530,002, filed Sep. 19, 1995, now U.S. Pat. No. 5,866,414, issued Feb. 2, 1999, a divisional of U.S. application Ser. No. 08/386,452, filed Feb. 10, 1995, now U.S. Pat. No. 5,695,998, issued Dec. 9, 1997.
Divisions (1)
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Number |
Date |
Country |
Parent |
09566497 |
May 2000 |
US |
Child |
10272679 |
Oct 2002 |
US |
Continuations (1)
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Number |
Date |
Country |
Parent |
09151790 |
Sep 1998 |
US |
Child |
09566497 |
May 2000 |
US |
Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
08530002 |
Sep 1995 |
US |
Child |
09151790 |
Sep 1998 |
US |
Parent |
08386452 |
Feb 1995 |
US |
Child |
09151790 |
Sep 1998 |
US |