Claims
- 1. An isolated polynucleotide comprising:
(a) a nucleotide sequence encoding a polypeptide having enoyl-ACP reductase activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2 or 8 have at least 86% sequence identity, or (b) the complement of the nucleotide sequence of (a).
- 2. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2 or 8 have at least 90% identity.
- 3. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2 or 8 have at least 95% identity.
- 4. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide comprises the amino acid sequence of SEQ ID NO: 2 or 8.
- 5. The polynucleotide of claim 1 wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO: 1 or 7.
- 6. A vector comprising the polynucleotide of claim 1.
- 7. A recombinant DNA construct comprising the polynucleotide of claim 1 operably linked to at least one regulatory sequence.
- 8. A method for transforming a cell, comprising transforming a cell with the polynucleotide of claim 1.
- 9. A cell comprising the recombinant DNA construct of claim 7.
- 10. A method for producing a plant comprising transforming a plant cell with the polynucleotide of claim 1 and regenerating a plant from the transformed plant cell.
- 11. A plant comprising the recombinant DNA construct of claim 7.
- 12. A seed comprising the recombinant DNA construct of claim 7.
- 13. An isolated polypeptide having enoyl-ACP reductase activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2 or 8 have at least 86% identity.
- 14. The polypeptide of claim 13, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2 or 8 have at least 90% identity.
- 15. The polypeptide of claim 13, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 2 or 8 have at least 95% identity.
- 16. The polypeptide of claim 13, wherein the amino acid sequence of the polypeptide comprises the amino acid sequence of SEQ ID NO: 2 or 8.
- 17. A method for evaluating at least one compound for its ability to inhibit enoyl-ACP reductase activity, comprising the steps of:
(a) introducing into a host cell the recombinant DNA construct of claim 7;(b) growing the host cell under conditions that are suitable for expression of the recombinant DNA construct wherein expression of the recombinant DNA construct results in production of enoyl-ACP reductase; (c) optionally purifying the enoyl-ACP reductase expressed recombinant DNA construct in the host cell; (d) treating the enoyl-ACP reductase with a compound to be tested; (e) comparing the activity of the enoyl-ACP reductase that has been treated with a test compound to the activity of an untreated enoyl-ACP reductase, and selecting compounds with potential for inhibitory activity.
Parent Case Info
[0001] This application claims the benefit of U.S. Provisional Application No. 60/303,309, filed Jul. 6, 2001. The entire content of the provisional application is herein incorporated by reference.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60303309 |
Jul 2001 |
US |