Claims
- 1. An improved method for introducing into the surface of indigo-dyed denim, localized areas of variation in color density and a high contrast between blue and white fibers of the denim, said method comprising laundering a denim material in the presence of pumice stone or an equivalent abrasive substance, wherein the improvement comprises, at a step in said method during which said pumice stones or an equivalent abrasive substance is added to achieve a stonewashed appearance, substituting for or adding to said pumice stones or equivalent abrasive substance by contacting the denim with:a. an effective amount of a redepositing cellulase sufficient to produce the variations in color density; and b. an effective amount of an added protease sufficient to reduce backstaining and increase the contrast between blue and white fibers in any order.
- 2. The method according to claim 1 wherein the effective amount of cellulase and protease is a ratio from about 5 ppm total redepositing cellulase protein: 1,000 ppm total added protease protein to 1,000 ppm total redepositing cellulase protein: 1 ppm total added protease protein.
- 3. The method according to claim 1 wherein said added protease is a serine or metallo protease.
- 4. The method according to claim 1 wherein the protease is from Bacillus or Aspergillus.
- 5. The method according to claim 1 further comprising the addition of a surfactant in an amount sufficient to further increase the removal of redeposited indigo dye.
- 6. The method according to claim 1 wherein the protease contacts the denim in a separate treatment water after the cellulase treatment.
- 7. The method according to claim 1 wherein the redepositing cellulase is from Trichoderma.
- 8. The method according to claim 1 wherein said added protease or said redepositing cellulase has been modified by genetic engineering to yield a strain which overexpresses particular components of the protease or cellulase or is deleted for specific components.
- 9. The method according to claim 1 wherein said added protease or said redepositing cellulase has been modified by protein engineering to yield protease or cellulase enzymes having altered properties such as enzyme activity or specificity.
- 10. The method according to claim 1 further comprising contacting the denim with pumice stones.
- 11. The method according to claim 3 wherein said serine protease is a subtilisin.
- 12. An improved method for introducing into the surface of indigo-dyed denim localized areas of variation in color density and a high contrast between blue and white fibers of the denim, said method comprising laundering a denim material in the presence of pumice stone or an equivalent abrasive substance, wherein the improvement comprises, at a step in said method during which said pumice stones or an equivalent abrasive substance is added to achieve a stonewashed appearance, substituting for or adding to said pumice stones or equivalent abrasive substance by contacting the denim with an effective amount of the composition comprising a redepositing cellulase and an added protease wherein the ratio of total protein in the redepositing cellulase to total protein in the added protease is from about 5 ppm: 1,000 ppm to 1,000 ppm: 1 ppm and wherein said redepositing cellulase and said protease are mixed together and incubated at a temperature ranging from about 30° C. to about 60° C. for about 1 hour to about 14 days such that the backstaining is reduced and the contrast between blue and white fibers is increased.
- 13. The method according to claim 1, wherein the redeposition is reduced by about 5% when measured on the backside of the denim compared to a method wherein protease is not added.
- 14. The method according to claim 1, wherein the redeposition is reduced by about 10% when measured on the backside of the denim compared to a method wherein protease is not added.
- 15. A method for reducing or preventing backstaining during the stonewashing treatment of indigo-dyed denim comprising the steps of:(a) substituting for or adding to pumice stones, or an equivalent abrasive substance, an effective amount of a redepositing cellulase and an added protease wherein the ratio of total protein of redepositing cellulase to total protein of added protease is from about 5 ppm: 1000 ppm to about 1000 ppm: 1 ppm; and (b) reducing or preventing backstaining wherein the contrast between white and blue is increased compared to indigo-dyed denim treated under the same conditions absent the added protease.
- 16. The method according to claim 15 wherein the redepositing cellulase is from Trichoderma.
- 17. The method according to claim 15 wherein the added protease is a serine protease.
- 18. The method according to claim 15 wherein the backstaining is reduced by 5% when measured on the backside of the denim compared to a method wherein the protease is not added.
- 19. The method according to claim 15 wherein the backstaining is reduced by 10% when measured on the backside of the denim compared to a method wherein the protease is not added.
- 20. The method according to claim 15 wherein said protease is added with the redepositing cellulose.
- 21. The method according to claim 15 wherein said protease is added after the redepositing cellulase.
Parent Case Info
This is a Divisional of application Ser. No. 08/159,401 filed Nov. 30, 1993 abandoned, which is a continuation-in-part of application Ser. No. 08/075,657 filed Jun. 11, 1993, now abandoned, which is a continuation-in-part of application Ser. No. 07/897,721 filed Jun. 12, 1992, now abandoned.
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Continuation in Parts (2)
|
Number |
Date |
Country |
Parent |
08/075657 |
Jun 1993 |
US |
Child |
08/159401 |
|
US |
Parent |
07/897721 |
Jun 1992 |
US |
Child |
08/075657 |
|
US |