Claims
- 1. A purified cycloisomaltooligosaccharide synthase, having the physicochemical properties of:
- (a.) action on a polymer of glucoses linked by .alpha.-1,6 linkages to form a cycloisomaltooligosaccharide of the formula: ##STR3## wherein n stands for an integer of 7-9, by intramolecular transglucosylation reaction;
- (b.) substrate specificity of acting on dextran having an .alpha.-1,6 linkage in its main chain, but not on amylopectin or pullulan; and
- (c.) optimum pH of about pH 5.5 and stability in the range of pH 4.5-8.5.
- 2. The synthase of claim 1 wherein the polymer of glucoses is dextran.
- 3. A process for producing cycloisomaltooligosaccharide synthase, which comprises culturing in a dextran-containing medium Bacillus sp. T-3040 (FERM BP-413; and then recovering said synthase from the culture.
Priority Claims (2)
Number |
Date |
Country |
Kind |
4-349444 |
Dec 1992 |
JPX |
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5-153456 |
Jun 1993 |
JPX |
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Parent Case Info
This is a continuation division, of application Ser. No. 08/174,596, filed Dec. 28, 1993, now U.S. Pat. No. 5,364,936.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4135977 |
Kenkyusho |
Jan 1979 |
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Non-Patent Literature Citations (2)
Entry |
Sundari et al., "Hydrophobic surfaces in oligosaccharides: linear dextrins are amphiphilic chains", Bioch. Biophys. Acta., 1065:35-41 (1991). |
Shiraishi et al., "Synthesis of Maltosyl(.alpha.1-6)cyclodextrins . . . ", Agric. Biol. chem., 53(8):3181-2188 (1989). |
Divisions (1)
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Number |
Date |
Country |
Parent |
174596 |
Dec 1993 |
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