Claims
- 1. A method for the immobilization of an enzyme which comprises covering the surface of a solid support with an enzyme and, then immobilizing the enzyme on said support by contacting said enzyme with an immobilizing reagent selected from the group consisting of an aldehyde and a polymerized aldehyde provided in the vapor phase to cause an immobilizing reaction.
- 2. The method for immobilization of an enzyme in accordance with claim 1, comprising the step of performing the immobilizing reaction under conditions where at least either a substrate for said enzyme or a coenzyme of said enzyme coexists with said enzyme.
- 3. The method for immobilization of an enzyme in accordance with claim 1 comprising the steps of covering the surface of a support with a mixture of said enzyme and a macromolecular substance, which forms a covalent bond through a reaction with the immobilizing reagent, together with the enzyme.
- 4. The method according to claim 1 wherein the enzyme is urease, alpha-amylase or alcohol dehydrogenase.
- 5. The method according to claim 1 wherein the immobilizing agent is 2-hydroxyadipaldehyde, crotonaldehyde, acrolein, glyoxal, propionaldehyde, paraformaldehyde or glutaraldehyde.
- 6. The method for immobilization of enzyme in accordance with claim 3, wherein said macromolecular substance has at least one functional group selected from the group consisting of: -NH.sub.2, >NH or -SH.
Priority Claims (2)
Number |
Date |
Country |
Kind |
54-62472 |
May 1979 |
JPX |
|
54-112569 |
Sep 1979 |
JPX |
|
Parent Case Info
This is a continuation of Ser. No. 152,149, filed May 21, 1980, now abandoned.
US Referenced Citations (4)
Continuations (1)
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Number |
Date |
Country |
Parent |
152149 |
May 1980 |
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