Enzyme preparation for catalyzing epimerization reaction of saccharide, method for producing epimerization reaction product, and epimerization reaction product

Abstract

An object of the present invention is to provide an enzyme preparation that efficiently catalyzes the epimerization reaction with little byproducts. The present invention provides an enzyme preparation for catalyzing an epimerization reaction of a saccharide, which contains any of the proteins (a) to (c) mentioned below, and wherein the saccharide is any selected from the group consisting of glucose, mannose, talose and galactose: (a) a protein consisting of the amino acid sequence of SEQ ID NO: 1 or 3; (b) a protein consisting of an amino acid sequence having an amino acid sequence identity of 90% or higher to the amino acid sequence of SEQ ID NO: 1 or 3, and having an activity for catalyzing an epimerization reaction of a saccharide; and (c) a protein consisting of an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 or 3 by substitution, insertion, deletion and/or addition of one or several amino acids, and having an activity for catalyzing an epimerization reaction of a saccharide.

Description

Claims

1. An enzyme preparation for catalyzing an epimerization reaction of a saccharide, which enzyme preparation contains any one or more of the-proteins (a) to (c) mentioned below, and wherein the saccharide is selected from the group consisting of glucose, mannose, talose and galactose: (a) a protein consisting of an amino acid sequence of SEQ ID NO: 1 or 3:(b) a protein consisting of an amino acid sequence having an amino acid sequence identity of 90% or higher to the amino acid sequence of SEQ ID NO: 1 or 3, and having an activity for catalyzing an epimerization reaction of a saccharide, and(c) a protein consisting of an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 or 3 by substitution, insertion, deletion and/or addition of one or several amino acids, and having an activity for catalyzing an epimerization reaction of a saccharide.

2. An enzyme preparation for catalyzing an epimerization reaction of a saccharide, which enzyme preparation contains a culture supernatant of a transformed cell into which any one or more of the polynucleotides (d) to (f) mentioned below has been introduced, and wherein the saccharide is selected from the group consisting of glucose, mannose, talose and galactose: (d) a polynucleotide consisting of a nucleotide sequence of SEQ ID NO: 2 or 4;(e) a polynucleotide that hybridizes with a complementary strand of the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2 or 4 under stringent conditions, and encodes a protein having an activity for catalyzing an epimerization reaction of a saccharide; and(f) a polynucleotide having a nucleotide sequence identity of 90% or higher to the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2 or 4, and encoding a protein having an activity for catalyzing an epimerization reaction of a saccharide.

3. A method for producing an epimerization reaction product of a saccharide, said method comprising a step of allowing the enzyme preparation according to claim 1 to act on a saccharide substrate to obtain the epimerization reaction product of a saccharide.

4. A method for producing a food, feed, feed for fish culture, cosmetic, or medicament, said method comprising a step of obtaining the food, feed, feed for fish culture, cosmetic, or medicament by using an epimerization reaction product of a saccharide obtained by implementing the method according to claim 3.

5. A method for producing an epimerization reaction product of a saccharide, said method comprising a step of allowing the enzyme preparation according to claim 2 to act on a saccharide substrate to obtain the epimerization reaction product of a saccharide.

6. A method for producing a food, feed, feed for fish culture, cosmetic, or medicament, said method comprising a step of obtaining the food, feed, feed for fish culture, cosmetic, or medicament by using an epimerization reaction product of a saccharide obtained by implementing the method according to claim 5.