Equipment Supplement for the National Center for Dynamic Interactome Research

Information

  • Research Project
  • 10392609
  • ApplicationId
    10392609
  • Core Project Number
    P41GM109824
  • Full Project Number
    3P41GM109824-08S1
  • Serial Number
    109824
  • FOA Number
    PA-20-272
  • Sub Project Id
  • Project Start Date
    8/1/2014 - 9 years ago
  • Project End Date
    4/30/2024 - a month ago
  • Program Officer Name
    WU, MARY ANN
  • Budget Start Date
    5/1/2021 - 3 years ago
  • Budget End Date
    4/30/2022 - 2 years ago
  • Fiscal Year
    2021
  • Support Year
    08
  • Suffix
    S1
  • Award Notice Date
    8/27/2021 - 2 years ago
Organizations

Equipment Supplement for the National Center for Dynamic Interactome Research

PROJECT SUMMARY Overall: The National Center for Dynamic Interactome Research. The emergent properties of life require the dynamic interactions of macromolecules, the two major classes of which are proteins and nucleic acids. These interactions form macromolecular machines and dynamic liaisons that shape the cell, transmit information and control cellular behaviors, and pathogenic alterations in molecular interaction networks (interactomes) underlie disease. Our understanding and modulation of biological systems, as well as their pathologies, thus relies on the ability to elucidate and interpret these interactions and their dynamics. For nucleic acids, recent advances have led to an explosion of genomic data. However, proteins are incredibly diverse in their abundance and their properties, making them highly versatile for their dynamic tasks, but at the same time exceptionally difficult to analyze. It is for these reasons that the interactomic revolution still lags very far behind the genomic revolution. The National Center for Dynamic Interactome Research (NCDIR) couples cell biology laboratories, a mass spectrometry laboratory, a systems biology laboratory, and a computational structural biology laboratory. The goal of the NCDIR is to synergistically pioneer new and improved approaches, integrating these technologies into a fundamentally unique ?pipeline? approach to address the urgent need of the biomedical community for technologies that can rapidly, reliably and routinely identify and characterize the dynamic cellular interactome. Our pipeline approach begins by developing technologies for purifying and preserving, with high fidelity, various defined forms of the hierarchical arrangement of interactors surrounding any chosen macromolecule. We then provide comprehensive, highly quantitative, detailed temporal and structural data for dynamic complexes. Such data is used to generate structural and mechanistic models that are predictive, testable, actionable, and guide experiments to focus on those that are most informative. The models aim to provide the biomedical community with the means for rational target-based future experimentation, functional characterizations and even intervention. These approaches will be developed, refined and beta-tested via a selected set of Driving Biological Projects and Collaborations that can enter and exit our pipeline at any point, and which present specific technological challenges. Critical to the design of the NCDIR is an effective training and dissemination program that is responsive to the urgent needs of the biomedical community.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    P41
  • Administering IC
    GM
  • Application Type
    3
  • Direct Cost Amount
    285578
  • Indirect Cost Amount
    0
  • Total Cost
    245598
  • Sub Project Total Cost
  • ARRA Funded
    False
  • CFDA Code
    859
  • Ed Inst. Type
    GRADUATE SCHOOLS
  • Funding ICs
    NIGMS:245598\
  • Funding Mechanism
    RESEARCH CENTERS
  • Study Section
    ZRG1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    ROCKEFELLER UNIVERSITY
  • Organization Department
    BIOLOGY
  • Organization DUNS
    071037113
  • Organization City
    NEW YORK
  • Organization State
    NY
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    100656399
  • Organization District
    UNITED STATES