Claims
- 1. An isolated nucleic acid molecule that is at least 85% identical to SEQ ID NO:1 ; SEQ ID NO:4; or SEQ ID NO:7.
- 2. An isolated nucleic acid molecule of claim 1, wherein the nucleic acid molecule encodes:
a yphC polypeptide comprising the amino acid sequence of SEQ ID NO:2, as depicted in FIG. 1; a yphC polypeptide comprising the amino acid sequence of SEQ ID NO:5, as depicted in FIGS. 2A-2B; or a yqjK polypeptide comprising the amino acid sequence of SEQ ID NO:8, as depicted in FIG. 3.
- 3. An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of:
(1) the sequence of SEQ ID NO:1, as depicted in FIG. 1, or degenerate variants thereof; (2) the sequence of SEQ ID NO:1, as depicted in FIG. 1, or degenerate variants thereof, wherein T is replaced by U; (3) nucleic acid sequences complementary to sequences of (1) and (2B); (4) nucleic acid fragments of sequences of (1), (2), and (3) that are at least 15 base pairs in length and which hybridize under stringent conditions to genomic DNA encoding the polypeptide of SEQ ID NO:2; (5) the sequence of SEQ ID NO:4, as depicted in FIGS. 2A-2B, or degenerate variants thereof; (6) the sequence of SEQ ID NO:4, as depicted in FIGS. 2A-2B, or degenerate variants thereof, wherein T is replaced by U; (7) nucleic acids complementary to (5) and (6); (8) the sequence of SEQ ID NO:7, as depicted in FIG. 3, or degenerate variants thereof; (9) the sequence of SEQ ID NO:7, or degenerate variants thereof, wherein T is replaced by U; (10) nucleic acid sequences complementary to sequences of (8) and (9); and (11) nucleic acid fragments of sequences of (8), (9), and (10) that are at least 15 base pairs in length and that hybridize under stringent conditions to genomic DNA encoding the polypeptide of SEQ ID NO:8.
- 4. An isolated nucleic acid molecule that is at least 15 base pairs in length and hybridizes under stringent conditions to SEQ ID NO:1 or SEQ ID NO:4.
- 5. A vector comprising a nucleic acid molecule of claim 1.
- 6. A host cell comprising an exogenously introduced nucleic acid molecule of claim 1.
- 7. An isolated polypeptide encoded by a nucleic acid molecule of claim 1.
- 8. A method for identifying an antibacterial agent, the method comprising:
(a) contacting a yphC or yqjK polypeptide with a test compound; and (b) detecting interaction of the test compound with the yphC or yqjK polypeptide, wherein interaction indicates that the test compound is an antibacterial agent.
- 9. A method of claim 8, further comprising:
(c) determining whether a test compound that interacts with the polypeptide inhibits growth of bacteria, relative to growth of bacteria cultured in the absence of a test compound that interacts with the polypeptide, wherein inhibition of growth indicates that the test compound is an antibacterial agent.
- 10. A method of claim 8, wherein the polypeptide is derived from a non-pathogenic bacterial strain.
- 11. A method of claim 8, wherein the polypeptide is derived from a pathogenic bacterial strain.
- 12. A method of claim 8, wherein the test compound is selected from the group consisting of polypeptides, ribonucleic acids, small molecules, and deoxyribonucleic acids.
- 13. A pharmaceutical formulation comprising an antibacterial agent identified by the method of claim 8, and a pharmaceutically acceptable excipient.
- 14. A method for treating a bacterial infection in an organism, the method comprising administering to the organism a therapeutically effective amount of the pharmaceutical formulation of claim 13.
- 15. The method of claim 14, wherein the organism is a human.
- 16. An antibody that specifically binds to a polypeptide of claim 7.
- 17. A method of claim 8, wherein the interaction is detected by detecting a decrease in function of the polypeptide contacted with the test compound; and further comprising:
(c) determining whether a test compound that decreases function of a contacted polypeptide inhibits growth of bacteria, relative to growth of bacteria cultured in the absence of a test compound that decreases function of a contacted polypeptide, wherein inhibition of growth indicates that the test compound is an antibacterial agent.
- 18. A method for identifying an antibacterial agent, the method comprising:
(a) contacting a nucleic acid encoding yphC or yqjK with a test compound; and (b) detecting interaction of the test compound with the nucleic acid, wherein interaction indicates that the test compound is an antibacterial agent.
- 19. A method of claim 18, further comprising:
(c) determining whether a test compound that interacts with the nucleic acid inhibits growth of bacteria, relative to growth of bacteria cultured in the absence of the test compound that interacts the nucleic acid, wherein inhibition of growth indicates that the test compound is an antibacterial agent.
- 20. A method for identifying an antibacterial agent, the method comprising:
(a) contacting an ortholog of a yphC or yqjK polypeptide with a test compound; and (b) detecting interaction of the test compound with the ortholog, wherein interaction indicates that the test compound is an antibacterial agent.
- 21. A method of claim 20, further comprising:
(c) determining whether a test compound that interacts with the ortholog inhibits growth of bacteria, relative to growth of bacteria cultured in the absence of the test compound that interacts with the ortholog, wherein inhibition of growth indicates that the test compound is an antibacterial agent.
- 22. A method of claim 21, wherein the ortholog is selected from the group consisting of B-yphC, B-yqjK, yfgK, and elaC.
- 23. A method of claim 21, wherein the ortholog is derived from a pathogenic bacterium.
- 24. A method for identifying an antibacterial agent, the method comprising:
(a) contacting an ortholog of a yphC or yqjK polypeptide with a test compound; (b) detecting a decrease in function of the ortholog contacted by the test compound; and (c) determining whether a test compound that decreases function of a contacted ortholog inhibits growth of bacteria, relative to growth of bacteria cultured in the absence of a test compound that decreases function of a contacted ortholog, wherein inhibition of growth indicates that the test compound is an antibacterial agent.
- 25. A method of claim 24, wherein the ortholog is selected from the group consisting of B-yphC, B-yqjK, yfgK, and elaC.
- 26. A method for identifying an antibacterial agent, the method comprising:
(a) contacting a nucleic acid encoding an ortholog of yphC or yqjK with a test compound; and (b) detecting interaction of the test compound with the nucleic acid, wherein interaction indicates that the test compound is an antibacterial agent.
- 27. A method of claim 26, further comprising:
(c) determining whether a test compound that interacts with the nucleic acid inhibits growth of bacteria, relative to growth of bacteria cultured in the absence of a test compound that interacts with the nucleic acid, wherein inhibition of growth indicates that the test compound is an antibacterial agent.
- 28. A method of claim 27, wherein the ortholog is selected from the group consisting of B-yphC, B-yqjK, yfgK, and elaC.
- 29. A method for treating a mammal having a Streptococcus pneumonia infection, the method comprising inhibiting the function of a yphC or yqjK polypeptide in Streptococcus pneumonia infecting the mammal.
- 30. An isolated polypeptide encoded by a nucleic acid molecule of claim 2.
- 31. An isolated polypeptide encoded by a nucleic acid molecule of claim 3.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application claims priority under 35 U.S.C. §119(e) from U.S. Ser. No 60/099,578, filed Sep. 9, 1998, which is incorporated herein by reference in its entirety.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60099578 |
Sep 1998 |
US |
Divisions (1)
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Number |
Date |
Country |
Parent |
09393858 |
Sep 1999 |
US |
Child |
10190279 |
Jul 2002 |
US |