Claims
- 1. An anti-sense expression vector capable of directing the transcription in a host cell of mRNA complementary to mRNA encoding the host cell's GRP78, said vector comprising a DNA sequence encoding host cell GRP78 peptide sequence operatively linked to an expression control sequence in reverse orientation such that transcription of the DNA produces the anti-sense mRNA.
- 2. A eucaryotic host cell for expressing a protein heterologous to the cell which comprises a host cell transformed with a vector of claim 1.
- 3. A yeast, fungal, insect, plant or mammalian host cell of claim 2.
- 4. A host cell of claim 2 which is additionally transformed with a vector capable of directing the expression of a protein heterologous to the cell.
- 5. A host cell of claim 4, wherein the protein heterologous to the cell is a factor VIII protein.
- 6. A method for producing a protein heterologous to a host cell by culturing a host cell transformed with a vector capable of directing the expression of the heterologous protein, the improvement comprising culturing a transformed host cell of claim 4 as the transformed host cell.
- 7. A method for producing a factor VIII protein which comprises culturing the improved host cell of claim 5 and recovering from the culture medium the Factor VIII protein so produced and secreted.
Parent Case Info
This application is a continuation in part of U.S. Ser. No. 931,381, filed 14 Nov. 1986 (now abandoned).
Non-Patent Literature Citations (5)
Entry |
Izant et al.; Science 229: 346 (1985). |
Lee et al.: J. Biolo. Chem. 258: 597 (1983). |
Lin et al.: Mol. Cell. Biol. 6: 1235 (1986). |
Attenello et al.; Science 226: 187 (1984). |
Toole et al.; Nature 312: 342 (1984). |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
931381 |
Nov 1986 |
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