Eukaryotic transposable element

Abstract

Disclosed are isolated transposable elements, or isolated DNA sequences which encode a transposase protein (or a portion of a transposase protein). The isolated transposable elements or the isolated DNA sequences being characterized by the ability to hybridize to the DNA sequence of Minos-1. The invention also relates to a purified transposase protein, or peptide fragments thereof, encoded by such DNA sequences. Such transposable are useful in methods for the stable introduction of a DNA sequence of interest into a cell. The invention further relates to transgenic animals produced by such methods. The sequence information disclosed herein is useful in the design of oligonucleotide primers which are useful for the isolation of related members of the Tc-1 family of transposable elements.

Description

BACKGROUND OF THE INVENTION

The Tc1-like family of transposons and the retroviral-like transposons are unique for their wide dispersion in diverse organisms. Six members belonging to the Tc-1-like family have been characterized in nematodes, diptera and fish: Tc1 in Caenorhabditis elegans, TCb1 in Caenorhabditis briggsae, HB1 in Drosophila melanogaster, Uhu in Drosophila heteroneura, Minos in Drosophila hydei and Tes1 in the Pacific hagfish Eptatetrus stouti. All are characterized by a relative short length (1.6 to 1.8 kb), the presence of inverted terminal repeats, and significant sequence similarity in the region between the repeats.

The Minos-1 transposable element has been identified as a 1775 bp dispersed repetitive sequence inserted within the transcribed spacer in one of the repeats of Drosophila hydei (Franz and Savakis, Nucl. Acids Res. 19:6646 (Dec. 11, 1991)). The element is characterized by 255-bp long perfect inverted repeats and the presence of two long, non-overlapping open reading frames (ORFs) on the same strand. The longest of the ORFs shows approximately 30% sequence identity with TcA, but does not begin with an ATG codon. It appears, therefore, that the cloned element represents a defective member of the Minos family, as is the case with all previously sequenced Tc1-like elements, with the possible exceptions of Tc1 and TCb1.

SUMMARY OF THE INVENTION

The invention relates to an isolated transposable element, or an isolated DNA sequence which encodes a transposase protein (or a portion of a transposase protein). The isolated transposable element or the isolated DNA sequence is characterized by the ability to hybridize to the DNA sequence of Minos 1 under stringent hybridization conditions. The invention also relates to a purified transposase protein, or peptide fragments thereof, encoded by such DNA sequences.

In another aspect, the invention relates to a method for the stable introduction of a DNA sequence of interest into a cell. This method involves the use of an isolated transposable element of the type described in the preceding paragraph, the isolated transposable element being modified to include the DNA sequence of interest flanked by the termini of the isolated transposable element. This modified transposable element is introduced into the cell in the presence of a transposase protein, or a DNA sequence encoding a transposase protein. The role of the transposase protein is to catalyze the transposition of the modified transposable element containing the DNA sequence of interest into the genomic DNA of the cell.

In a third aspect, the invention relates to a method for isolating members of the Tc-1 family of transposable elements from genomic DNA of a eukaryote of interest. According to this method, oligonucleotide primers are provided which are complementary to a sequence of at least about 12 consecutive nucleotides which encode amino acids which are highly conserved in aligned sequences of nematode Tc-1 family members and Minos family members. These oligonucleotide primers are used to prime amplification by the polymerase chain reaction (PCR). The amplification products are then used to isolate DNA encoding the entire Tc-1 family member from the eukaryote of interest by conventional methods.

In a fourth aspect, the invention relates to a transgenic animal. The transgenic animal is produced by a method which involves the use of an isolated transposable element characterized by the ability to hybridize to the DNA sequence of Minos 1, the isolated transposable element being modified to include the DNA sequence of interest flanked by the termini of the isolated transposable element. This modified transposable element is introduced into a cell in the presence of a transposase protein, or a DNA sequence encoding a transposase protein.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A-1C is a diagram providing the consensus sequence of elements Minos-1, Minos-2 and Minos-3 with nucleotide deletions after nucleotides 365, 678 and 715. The terminal inverted repeats and the intron sequence are shown in small letters. Differences between the three elements are indicated above and below the nucleotide sequence. More specifically, nucleotide 896 is a G in Minos-2 and Minos-3 and an A in Minos-1. Nucleotide 1157 is a C in Minos-1 and Minos-3 and a T in Minos-2.

FIGS. 2A-2C is a diagram providing the consensus sequence of elements Minos-1, Minos-2 and Minos-3. The terminal inverted repeats and the intron sequence are shown in small letters. The first and last nucleotides of the sequence, A and T, respectively, are generated by a duplication of the chromosomal target site TA during insertion of the element. The deduced amino acid sequence of two open reading frames is shown above the nucleotide sequence. Differences between the three elements are indicated above and below the nucleotide sequence. More specifically, nucleotide 900 is a G in Minos-2 and Minos-3 and an A in Minos-1. Nucleotide 1161 is a C in Minos-1 and Minos-3 and a T in Minos-2. Amino acid residue 148 is a tryptophan in Minos-2 and Minos-3 and a stop codon in Minos-1. Amino acid residue 235 is a serine in Minos-1 and Minos-3 and a leucine in Minos-2.

FIG. 3A is a diagram of the insert of the transposon plasmid pMihsCcw. ML and MR signify the left- and right-end parts of Minos, respectively. Speckled boxes indicate the D. melanogaster Hsp70 promoter (Hsp70-P) and terminator (Hsp70-T) sequences. Wide hatched bars indicate the Minos (M) and Medfly white (W) sequences that were used as probes for the analysis of transformants.

FIG. 3B is a diagram of the insert of the Minos helper plasmid pHSS6hsMi. Speckled box indicates the D. melanogaster Hsp70 promoter (Hsp70-P) sequence. Salient restriction sites are shown. Exon 1 and exon 2 are also referred to herein as open reading frame 1 (ORF1) and open reading frame 2 (ORF2), respectively. IR indicates the right-hand terminal inverted repeat.

FIG. 4 is a bar graph depicting the frequencies of transformants among G1 progeny. Bars indicate the numbers of G1 flies from the individual cages. The sex of the G0 flies in each cage is indicated. The numbers above cages 1, 3, 25 and 33 indicate the w.sup.+ flies that were recovered from these cages.

SEQUENCE LISTINGS CROSS-REFERENCES

In portions of the Specification, the following sequence listing cross-reference is applicable:

SEQ ID NO: 1 Nucleic acid sequence of Minos-1 with nucleotide deletions after nucleotides 365, 678 and 715. SEQ ID NO: 2 Nucleic acid sequence of Minos-2 with nucleotide deletions after nucleotides 365, 678 and 715. SEQ ID NO: 3 Nucleic acid sequence of Minos-3 with nucleotide deletions after nucleotides 365, 678 and 715. SEQ ID NO: 4 Nucleic acid sequence of Minos-1. SEQ ID NO: 5 Deduced amino acid sequence of Minos-1. SEQ ID NO: 6 Nucleic acid sequence of Minos-2. SEQ ID NO: 7 Deduced amino acid sequence of Minos-2. SEQ ID NO: 8 Nucleic acid sequence of Minos-3. SEQ ID NO: 9 Deduced amino acid sequence of Minos-3. SEQ ID NO: 10 MVWGC. SEQ ID NO: 11 WPSQSPDL. SEQ ID NO: 12 WPSNSPDL.

DETAILED DESCRIPTION OF THE INVENTION

The invention disclosed herein is based on the initial discovery of Minos-1, an apparently defective member of the Tc-1 family of transposable elements. This 1779-bp element is characterized by perfect inverted repeats of 255-bp at each termini. The sequence encodes two non-overlapping reading frames, one of which has significant similarity with the putative transposase encoded by the transposable element Tc1 of Caenorhabiditis elegans. However, the Minos-1 element, because of a stop codon within the putative transposase gene, apparently cannot encode an active transposase.

In an effort to identify sequences related to the Minos-1 sequence, genomic DNA of D. hydei was probed with a portion of the Minos-1 sequence under stringent hybridization conditions. As discussed in detail in the Exemplification section which follows, two full-length related sequences were identified, both of which encode an active transposase.

Isolated Nucleic Acids and Uses Thereof

Thus, in one aspect, the subject invention relates to an isolated transposable element which hybridizes to the DNA sequence of Minos-1 under stringent hybridization conditions. As used herein, stringent hybridization conditions are considered to be hybridization in a buffered solution of 0.9 M NaCl at 55.degree. C. In D. hydei there are up to 30-copies detected which hybridize to Minos thus, it is likely that a large number of variants can be isolated using these conditions. Comparable hybridization stringency can be established at other salt concentrations and temperatures. This is accomplished, for example, by the inclusion of organic denaturants such as formamide in the hybridization buffer. DNA sequences which hybridize to the Minos-1 sequence under stringent hybridization conditions are referred to herein as members of the Minos family of transposable elements. DNA sequences which hybridize to the Minos-1 sequence under stringent hybridization conditions include, for example, the Minos-2 and Minos-3 DNA sequences. Other examples of DNA sequences which hybridize to the Minos-1 sequence under stringent hybridization conditions include Minos-1, Minos-2 and Minos-3 DNA sequences having base deletions, insertions and/or substitutions.

The term transposable element, as used herein, refers to a DNA sequence whose excision from/insertion into genomic DNA is catalyzed by a functional transposase protein encoded by a non-defective member of the Minos family of transposable elements. A member of the Minos family which encodes a functional transposase and possesses other necessary cis-acting elements (e.g., inverted terminal repeats) falls within this definition. In addition, a transposable element which encodes a defective transposase (e.g., Minos-1 itself) falls within this definition. As discussed in greater detail below, such defective transposable elements can be used in conjunction with a helper element (i.e., a member of the Minos family which encodes a functional transposase) to introduce a DNA sequence of interest into a cell (e.g, a eukaryotic cell such as an animal, plant or yeast cell or a prokaryotic cell such as a bacterial cell).

The invention also relates to an isolated DNA sequence encoding a functional transposase protein, or a portion of a transposase protein, encoded by a member of the Minos family. Such a DNA sequence need not retain the ability to transpose in the presence of the encoded transposase protein. A sequence encoding a functional transposase protein can be used to prepare an expression construct which can be used to produce the transposase protein by recombinant DNA methodology. Such a recombinant protein can be over-produced in a eukaryotic (e.g., yeast) or prokaryotic host cell (e.g., E. coli), and subsequently purified by conventional methods.

The active transposase can be used in a variety of ways. For example, as discussed below, the transposase can be co-introduced into a eukaryotic cell with a modified transposon carrying a DNA sequence of interest to catalyze the insertion of the modified transposon into the genomic DNA of the eukaryotic cell. This is an alternative to the co-introduction of a helper construct in eukaryotic cells which do not constitutively produce the Minos transposase.

In addition, the transposase, or portions thereof, can be used to produce antibodies (monoclonal and polyclonal) reactive with the transposase protein. Methods for the production of monoclonal and polyclonal antibodies are straightforward once a purified antigen is available.

Through the isolation and DNA sequence analysis of additional members of the Minos family, refinement of the consensus sequence of FIGS. 2A-2C is possible. This refined consensus sequence can be used to predict modifications of the transposase protein which will affect the specific activity of the transposase. Such predictions are easily tested by modifying the DNA sequence of an expression construct encoding the transposase by site-directed mutagenesis to either bring the sequence into a greater degree of conformance with the consensus sequence, or a lesser degree of conformance with the consensus sequence. The affect of such changes on the activity of the transposase protein are monitored by assessing the affect of the mutation on transposition frequency catalyzed by the recombinant transposase.

Methods for the Introduction of DNA Sequences into a Cell

Transposable elements of the Minos family, and the active transposase encoded by such elements, are useful in methods for introducing a DNA sequence of interest into a cell (e.g., a eukaryotic cell such as an animal, plant or yeast cell or a prokaryotic cell such as a bacterial cell). Typically, the DNA sequence of interest will be a gene which encodes a protein. Such a gene can be placed under the regulatory control of a promoter which can be induced or repressed, thereby offering a greater degree of control with respect to the level of the protein in the cell. In addition to a DNA sequence encoding a protein, any other DNA sequence can be introduced by this method including, for example, regulatory sequences.

The Minos transposable elements can be used to introduce a DNA sequence of interest into the cells of invertebrates. For example, the Minos transposable elements can be used to introduce a DNA sequence of interest into the cells of arthropods. Arthropods include, for example, crustaceans, arachnids, myriapods and insects.

The Minos transposable elements can be used to introduce a DNA sequence of interest into either germ line or somatic cells. The introduction of DNA into germ line cells has the significant advantage that the DNA sequence of interest will be contained in all cells of the mature organism and transmitted to progeny.

The Minos transposable element has been demonstrated to function in a species which is separated from the Minos source species by an evolutionary distance of 40 million years. This represents the first demonstration of a mobile element which can function autonomously in the germ line of eukaryotes separated by such an evolutionary distance and is likely to lead to the development of a long-sought transformation system applicable across taxonomic barriers.

However, even within the dipteran class, significant important applications for the Minos element exist. Listed below are examples of a variety of plant and animal pests, and human disease vectors which fall within the dipteran genus.

______________________________________ COMMON NAME______________________________________AGRICULTURAL PESTSCeratitis capitata MedflyAnastrepha species Carribean fruit flyDacus oleae DacusBactrocere species Oriental fruit flyANIMAL PESTSCochliomya hominivorax Screw Worm FlyLucilia cuprina Sheep blowflySimulium species Black flyHUMAN DISEASE VECTORSAnopheles species mosquitoAedes species mosquitoMusca domestica house fly______________________________________

Methods currently employed to control the populations of certain members of the dipteran class include the release of sterile males. An example of the utility of the germ line transformation methods of this invention includes the improvement of the existing release method. The methods of this invention can be used to improve such methods by enabling sexing schemes and for developing strains with desired characteristics (e.g., improved viability in the field), conditional lethal genes for improved safety, and visible or molecular genetic markers for monitoring. Genetic sexing, i.e. the capability of selectively killing the females (or transforming them into males) in mass-rearing facilities, is recognized as an important need presently. Rearing and releasing only males has several advantages including lower breeding cost and the avoidance of population explosions due to inadvertent release of non-sterilized insects.

For example, the Mediterranean fruit fly (Medfly) Ceratitis (C.) capitata is a major agricultural pest for many fruit species that is geographically widespread in tropical and temperate regions. The Medfly has been introduced relatively recently into the New World, and appears to be spreading rapidly, threatening fruit producing areas in North America (Carey, J. R., Science, 253:1369 (1991)). Since the mid 1970's, the sterile insect technique has been used successfully for Medfly eradication and control. This method relies on the decrease in or collapse of fly populations following releases of large numbers of sterile insects over infested areas, and offers an environmentally attractive alternative to massive spraying with insecticides (Knipling, E. F., Science, 130:902 (1959)). The germ line transformation methods of this invention can be used to improve the sterile insect technique by, for example, enabling sexing schemes. The germ line transformation methods of this invention can also be used for developing Medfly strains with desired visible markers that can be used for monitoring effective population control.

The methods are also useful for insects for which it might be desirable to introduce new traits in the genetic pool, rather than controlling the population levels. For example, the presence of several sympatric sub-species of Anopheles gambiae, all of which transmit malaria, makes it highly unlikely that population control with biological methods such as the sterile insect technique will work. An alternative scheme might involve spreading genes for refractoriness to parasite infection into the existing populations of Anopheles through the use of transposable elements. Population dynamics simulations indicate that this can be effected by releasing relatively small numbers of individuals carrying an autonomously transposing element.

The element may be actively transposing in other taxa (e.g. vertebrates) under the appropriate conditions thus, it will be recognized by those skilled in the art that the methods disclosed herein relating to diptera can be extended to higher eukaryotes. If the transposase is functional when expressed or otherwise introduced in vertebrate embryos or cells, it is possible to develop transformation methods based on Minos elements for non-insect species as well.

A transposon-based method for producing transgenic animals or for stably transfecting cells in vitro has very important advantages compared to the methodology presently used. For example, stable integration of DNA into the germline of several mammals is now routinely achieved by micro-injecting linear DNA molecules into the nucleus of early embryos. Some of the animals that develop from injected embryos are mosaics for integration events and in only a fraction of these the germ line is involved. Moreover, most events consist of integration of tandem repeats of the injected DNA; single-insertion events do occur at higher frequencies relative to tandem insertions if DNA is injected at lower concentrations, but at a considerable cost in time and expense because the overall transformation frequencies drop.

Using a defined transposon-transposase system may overcome some or all of these problems. First, as in Drosophila, it may not be necessary to have to inject the DNA into the nucleus. If a mixture of transposon plus helper plasmids (or transposon plus purified transposase) is active when introduced into the cytoplasm, it may be possible to replace costly and time-consuming micro-injection with other methods, such as use of liposomes. Second, by controlling the relative transposon/transposase levels it may be possible to improve the overall efficiency, with a parallel increase of the frequency of single-insertion events.

Methods for the introduction of the Minos transposon into germ line cells of diptera are analogous to those previously used in connection with other transposable elements (see, e.g., Drosophila, A Laboratory Handbook, Ashburner, M., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989)). Briefly, the most common approach is to employ a carrier/helper transposon system. The carrier transposon is a Minos transposon which has been modified by the insertion of a DNA sequence of interest in the region of the transposon flanked by the inverted terminal repeats. Typically, sequences relating to the transposase function are deleted in order to accommodate the DNA of interest. The helper transposon is a Minos transposable element which encodes an active transposase. The transposase catalyzes the transposition of the carrier transposon into the genomic DNA of the germ line eukaryotic cells. Typically, the helper and carrier are microinjected into the posterior pole of pre-blastoderm embryos, where the precursor cells of the germ line develop.

An alternative to the helper/carrier system involves the purification of active transposase (for example, from an E. coli culture transformed with a recombinant construct encoding the Minos transposase). The purified transposase can be co-injected into appropriately selected cells along with a carrier transposon to effect integration of the carrier into the recipient genome.

The compositions and methods of this invention are also useful for the introduction of a DNA sequence of interest into mammalian somatic cells. Typically this is accomplished in a manner analogous to the methods described in connection with germ line cells (e.g., helper/carrier systems are employed). Somatic cell introduction is typically carried out using cells grown in culture and DNA can be introduced, for example, by calcium co-precipitation or other conventional methods.

Methods for Isolating Additional TC-1 Family Members

DNA sequence analysis of the members of the Minos family disclosed herein, and comparison of this sequence information to the sequences of Tc-1 family members from evolutionarily distant organisms (e.g., nematode), reveal short stretches of conserved amino acid sequence within the transposase coding region. This high degree of conservation suggests a method for isolating Tc-1 family members from diverse eukaryotic species.

This method involves the amplification of DNA by polymerase chain reaction from a eukaryote of interest using primers which are complementary to a sequence of at least about 12 consecutive nucleotides which encode amino acids which are highly conserved in aligned sequences of nematode Tc-1 family members and dipteran Minos family members. Such amino acid sequences include, for example, MVWGC (SEQ ID NO:10), WPSQSPDL (SEQ ID NO:11) and WPSNSPDL (SEQ ID NO:12).

Exemplification

Materials and Methods

Fly Strains

Standard procedures were used for culturing of Drosophila hydei. All strains used in this study have been used previously for rDNA work and are named for the X and Y chromosomes. Strain bb.sup.1 (bb.sup.1 /bb.sup.1 .times.bb.sup.1 /Y) carries a bobbed X chromosome; strain X.sup.7 (X.sup.7 /X.sup.7 .times.X.sup.7 /Y) is a subline of the Dusseldorf wild-type strain; strain X X/Y(X X/Y.times.X/Y) females carry a compound X chromosome which has no rDNA. Strain wm1/Y (wm1/Y.times.X-3/Y) females have a compound X chromosome (wm1); males carry a X-autosome 3 translocation which has no rDNA.

DNA Manipulations and Sequencing

All basic procedures were carried out essentially as described (Maniatis et al. 1982). DNA from adult females of strain bb.sup.1 was partially digested with EcoRI and cloned into phage vector .lambda.gt7. To recover new Minos elements, the library was screened by hybridization with a 1.7 kb HhaI fragment which contains most of the Minos-1 sequence. For sequencing, the appropriate restriction fragments from positive clones were subcloned into plasmid vectors pUC8 and pUC9 and nested deletions were generated by digestion with exonuclease Bal31 followed by subcloning. Sequencing was performed by conventional methods. Both strands were sequenced, with a minimum of two independent sequences for each base pair.

Sequence Analysis

Database searches and sequence analysis and manipulations were performed using programs FASTA (Pearson and Lipman, Proc. Natl. Acad. Sci. USA, 85:2444-2448 (1988)). BLAST (Altschul et al., J. Mol. Biol., 215:403-410 (1990)) and the computer package GCG (Devereux et al., Nuc. Acids Res., 12:387-395 (1984)). The program CLUSTAL (Higgins and Sharp, 1988) was used for protein sequence alignments.

Results

The Sequence of Minos

Three new representatives of the Minos family of transposable elements have been cloned and sequenced; they have been named Minos-2, Minos-3 and Minos-4, Minos-1 being the element reported previously. Minos-2 and Minos-3 are complete elements distinct from Minos-1, as judged from the restriction maps of the flanking DNA and the flanking sequences. The sequences of the elements, summarized in FIGS. 2A-2C, show very little variation, differing in only two positions. At position 900 of the sequence, Minos-2 and Minos-3 have a G instead of the A found in Minos-1. This transition changes a TAG stop codon to TGG and restores a 603 bp ORF beginning with ATG at position 878. The second difference is at nucleotide 1161, which is a C in Minos-1 and Minos-3 and a T in Minos-2. This causes a ser.fwdarw.leu substitution in ORF2 of Minos-2, relative to Minos-1 and Minos-3. Minos-2 and Minos-3, therefore, have two complete ORFs beginning with an ATG; ORF1, which can encode a 133 amino-acid peptide, and ORF2, which can encode a 201 amino-acid peptide.

The Minos-4 clone does not contain a complete element. The sequence of the cloned DNA fragment begins at the EcoRI site found at position 1172 of the other members and is identical to the Minos-1 sequence to base 1779. Apparently Minos-4 represents a partial isolate rather than a defective member of the family, since the library from which it was isolated was from DNA cut with EcoRI.

The DNA sequence flanking the cloned elements are different from each other; this indicates that these elements are inserted at different sites of the D. hydei genome, and are, therefore, distinct. These sequences are mainly characterized by a high A/T content, and do not show any other obvious similarity. In all cases, the inverted repeats end with the dinucleotide TA, which is at the same time a direct and an inverted repeat. Because of this, there is some ambiguity in defining the ends of the element precisely. Shown below are the sequences of the Minos 1-4 insertions sites. The rDNA sequences flanking the Minos elements are shown in lower case and Minos sequences are shown in upper case. The rDNA sequence identical to the flanking DNA of Minos-1 has been aligned with the Minos-1 insertion sequence. It is noted that since gapped sequences are treated as separate sequences for purposes of the Rules of Practice in Patent Cases (37 CFR 1.822(o)), and since each of the separate sequences contain less than 10 nucleotides, the sequences shown below have not been listed in the Sequence Listing.

In the case of Minos-1, which is inserted into a region which has been previously sequenced, the external transcribed spacer of the rDNA repeat, there are two possibilities. As shown below, deleting the sequence which begins with ACGA and end with TCGT would restore the rDNA sequence; the element, with an A and a T at the two ends may have inserted between a T and an A. In this possibility, the element would be 1779 bp long with 255 bp inverted repeats. Alternatively, the element may begin and end with CGA . . . TCG and produce a target site duplication, as happens with many other mobile elements. In this possibility the target site duplication would involve the dinucleotide TA, and the size of the element would be 1777 bp. For numbering, the A of the TA repeat has been designated nucleotide number 1 of the Minos-1-3 sequences.rDNA ataat--------------------attaaMinos-1 ataatACGA------------TCGTattaaMinos-2 aatatACGA------------TCGTataatMinos-3 gctttACGA------------TCGTagaagMinos-4 tttctACGA .vertline. .vertline. 1775 1

Mobility and Homogeneity of Minos Elements

The striking degree of sequence conservation among the cloned Minos elements suggests that, as in the case of Tc1, all Minos elements may be highly homogeneous. To test this the single HhaI site within each of the terminal repeats of Minos was exploited. The 1.68 kB HhaI fragment of Minos-1 was used as probe in a Southern blot of genomic DNA from the same strains, digested with CfoI, an isoschisomer of HhaI. A single, strong band of approximately 1.7 kb was detectable in all lanes, indicating that no major deletions or rearrangements are present in the Minos elements present in these strains.

Comparison of the Proteins Encoded by TC-1 and Minos

The deduced 201 amino acid sequence of the ORF2 in Minos-2 and Minos-3 shows significant sequence similarity with the 201 carboxy terminal residues of TcA, the putative transposase of Tc1; alignment of the sequences gives 63 identities (31%) and 91 conservative substitutions (45%) with only two single-residue insertion-deletions. The two sequences, however, differ in size; TcA has 72 additional amino acids at the amino end. The 50 amino-terminal residues of TcA show weak but significant sequence similarity with the carboxy terminus of Minos ORF2; introduction of a 60-bp deletion in the Minos DNA sequence creates a long open reading frame which contains most of ORF1 (codons 1 to 138) and the entire ORF2 extended by 22 codons upstream of the ATG. Interestingly, this 60-bp sequence, from base 752 to base 811 of the Minos sequence, exhibits features of an intron. More specifically, the 5' and 3' ends conform to the consensus splice donor and acceptor sites and a version of the internal splice signal consensus is found 30 nucleotides upstream from the 3' end.

Divergence of the TCA-Related Sequences

Although Minos inhabits a Drosophila species, it is not more related to the other Tc1-like elements from Drosophila species, HB1 and Uhu. These elements, or at least the members which have been sequenced, do not contain open reading frames comparable in length to that of Tc1. However, if small numbers of deletions and insertions are introduced in their DNA sequences, open reading frames can be generated which show significantly similarity with the TcA sequence. Most of these insertion-deletion changes involve one nucleotide, presumably representing mutations which have accumulated in these inactive elements. Table 1 shows a similarity matrix between the three Drosophila and the two nematode elements, in the regions corresponding to the hypothetical Minos exon 2. In Table 1, percent identities are shown above the diagonal; identical/total positions are shown below the diagonal. Minos shows approximately the same degree of similarity (between 28 and 36 percent identity) with all the other elements; HB1 and Uhu show comparable similarities. In a multiple sequence alignment of the same regions, 21 of the resulting 225 positions (9%) are invariant and 49 positions (22%) are occupied by related amino acids. It should also be noted that the similarity between HB1 and Uhu with Tc1 and Minos extends another 18 codons upstream from the position corresponding to the first codon of the hypothetical exon 2 of Minos. No other significant similarities can be detected between Tc1, Uhu, HB1 and Minos in the sequences between the terminal repeats.

TABLE 1______________________________________TC1 TCb1 Minos Uhu HB1______________________________________Tc1 71 31 44 33TCb1 160/223 34 41 35Minos 70/221 75/222 36 28Uhu 96/217 89/217 78/218 31HB1 73/223 79/223 62/222 68/219______________________________________ The ORF1 Sequence is Related to the Paired Box Sequence

Searches of the nucleic acid and protein sequence data libraries with the ORF1 sequence using the FASTA and WORDSEARCH algorithms gave no significant matches. However, the Basic Local Alignment Search Tool program revealed a similarity with the paired box sequence, a peptide sequence found in the Drosophila paired gene product, and conserved in other Drosophila and mammalian genes. This similarity extends approximately between residues 1 to 96 of the Minos sequence, and residues 35 to 131 of the Drosophila paired protein. Alignment of the Minos sequence with the Drosophila and human paired box sequences for maximum similarity shows 16 invariant positions in this region (17%) and 49 positions occupied by related amino acids (51%). The corresponding values for the human and Drosophila paired sequences are 72% identities and 23% conserved positions.

Although the Minos-paired similarity is weak compared to that between the Drosophila and human paired sequences, it is statistically significant. The similarity scores between the Minos sequence (amino acids 1 to 118 of ORF1) to the corresponding human paired sequence (amino acids 17 to 135 of the published sequence) is approximately 10 standard deviations higher than the average of the scores obtained from 50 comparisons made between the Minos sequence and 50 randomly shuffled human paired sequences.

Transposition in D. Melanogaster

A D. melanogaster "helper" strain which can overproduce the Minos transposase upon exposure to heat shock was constructed. The strain was constructed by introducing a modified Minos element into the germ line by conventional P element transformation (see, e.g., Drosophila, A Laboratory Handbook, Ashburner, M., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989)). To place the Minos transposase under heat shock control, the left-hand terminal repeat of Minos-2 was replaced by the D. melanogaster hsp 70 promoter. This modified element was inserted into the P element transformation vector pDM30, which contains a wild-type copy of the Drosophila rosy (ry) gene as a dominant visible marker. The plasmid (pPhsM2) was injected into pre-blastoderm embryos of a ry strain, injected GO adults were mated to ry flies and ry.sup.+ G1 progeny were bred further. Three independent transformants were recovered, two on the third chromosome (named M46 and M67) and one on the X (M84). Southern blots using ry and Minos probes indicated that each of the three transformants contains a single insertion of the complete sequence between the P element ends. Northern blots of total RNA from adult transformed flies subjected to a heat shock showed abundant transcripts hybridizing to Minos probes. No Minos-related transcripts have been detected by the same probes in RNA from non-heat shocked flies. The structure of the RNA transcripts was investigated in another series of experiments discussed below.

Breeding of these transformants showed that they are all homozygous lethal. This observation was unexpected; the recovery of recessive lethal mutations due to insertional inactivation of essential genes is a rather uncommon event in P transformation experiments. Moreover, the insertion into the X clearly has not caused a "knock-out" mutation since hemizygous males are viable and fertile; only homozygous females are inviable. This behavior suggested that the lethality may be dosage- or pairing-dependent; the latter being more likely because double heterozygotes of the two insertions in the 3rd chromosome are viable. The observed lethality is a useful feature which enables one to follow the segregation of the "helper" chromosomes by keeping them over genetically marked balancers.

Strong evidence for Minos transposition in the germ line was obtained by first introducing the M67 chromosome into a white background (y,w; TM3/M67). Pre-blastoderm embryos were injected with a plasmid (pM2w) containing a complete Minos-2 element with a wild-type copy of the white (w) gene inserted into its unique EcoRI restriction site within ORF2. The inserted w sequences provide a dominant selectable marker; in addition they interrupt ORF2, making the production of active transposase from this construct highly improbable. Three separate experiments were conducted: In experiment A injected embryos and the developing larvae and adults were kept at 18.degree. C., in experiment B they were kept at 25.degree. C. throughout development, and in experiment C the embryos were subjected to a 1-hour 37.degree. C. heat shock three hours after injection. All emerging GO flies (63, 38 and 61, from experiments A, B and C, respectively) were mated to y,w; TM3/Dgl3 flies and the progeny were scored for the appearance of the w.sup.+ phenotype. To date, at least four independent germ line transformation events have been detected in experiments A and B. Two of these events come from a single GO male from experiment A and at least two have been recovered from two different GO flies from experiment B. The results are shown in Table 2 below:

TABLE 2______________________________________ InsertionExperiment GO #G1 Scored w.sup.+ Chromosome______________________________________A A10 286 A10.1 X A10.2 3 A10.3 3 A10.4 -- A10.5 -- A10.6 --B B13 75 B13.1-3 --C B33 116 B33.1-18 --______________________________________

Evidence that the Minos-w.sup.+ transposon can be mobilized in the soma of flies which produce the transposase has been obtained. Larvae of the constitution y,w; TM3/[M2w]M67 (progeny of the A10.2 fly), which contain both transposon and helper sequences, were subjected to heat shock and adult flies were examined for the appearance of eye color mosaicism. More than 50% of the flies showed mosaicism of different degrees. Patches of ommatidia with either reduced or increased pigmentation were observed which is consistent with the expected result of a somatic deletion or transposition event. No mosaicism has been detected in flies not subjected to a heat shock at the larval stage. The somatic instability results clearly indicate that the w.sup.+ insertions are minos-mediated.

Ananysis of Minos mRNA Transcripts

Total RNA was isolated from the M67 strain, the construction of which is described above. The structure of mRNA transcripts was investigated by the polymerase chain reaction (PCR) method of DNA amplification. A particularly important aspect of this investigation was to determine the status of the 60 base pair putative intron region (discussed above) in the mRNA transcripts. As was mentioned previously, this sequence is characterized by 5' and 3' ends which conform to the consensus splice donor and acceptor sites, and has a version of the internal splice signal consensus sequence 30 nucleotides upstream from the 3' end.

To determine the status of this putative intron, PCR priming sites were selected from exon sequences (ORF1 and ORF2) flanking the putative intron. The PCR product synthesized in this reaction was cloned and sequenced by conventional methods. The sequencing experiments revealed unambiguously that the 60 base pair intron sequence was, in fact, absent in the amplified DNA.

The removal of the 60-bp sequence in the correctly spliced primary transcript initiating upstream from ORF1, results in the generation of a 1023-bp open reading frame which encodes a peptide of 341 amino acids. An alignment of the 273 carboxy-terminal amino acids of this peptide with the sequences of TcA and the 273-residue hypothetical peptide of TCb1 was generated by the multiple alignment program CLUSTAL, which introduces gaps in the sequences to achieve maximum sequence similarity. The three sequences were aligned without the need of any insertions-deletions (with the exception of the two one-residue gaps required for optimal alignment in the ORF2 region) and show an overall 28% identity, i.e. 76 of the 273 positions are invariant. In the region upstream from the first methionine of ORF2, twelve out of seventy two positions (16%) are invariant; 29 positions (40%) are occupied by structurally related amino acid residues. Although this degree of similarity is lower than that in the ORF2 region, it is statistically significant.

The sequence similarity between TcA and the carboxy end of the Minos hypothetical protein is also reflected in their secondary structures. Comparisons of .alpha.-helix and .beta.-sheet predictions and hydrophobicity profiles between the Tcl and Minos sequence show similarities in several regions. Another feature of the sequences is their high content, approximately 20%, in basic amino acids. TcA has 29 arginines, 16 lysines and 11 histidines, and the TcA-related Minos sequence has 20 arginines, 32 lysines and 4 histidines. These are more abundant at the amino-terminal half of both sequences, although the position of most is not strictly conserved. The proteins are fairly basic, with computed isoelectric points of 11.27 for TcA and 10.73 for the related Minos peptide. The computed pI of the complete hypothetical 361 amino acid Minos protein is 10.97.

Gene Transfer into C. capitata Using Minos Transposable Elements.

Single copies of exogenous DNA can be introduced into the genome of C. capitata by using a germ line transformation system which utilizes the transposable element Minos to mediate precise integration of DNA at acceptable frequencies.

To provide an effective dominant selectable marker for detection of transformants, an approximately 3.7 kb NotI fragment containing the wild-type white cDNA of C. capitata, flanked by the D. melanogaster hsp 70 promoter and terminator sequences, was inserted into the NotI site of the Minos vector pMiNot which was constructed by replacing a 644 bp MscI fragment of the Minos transposase gene (nucleotides 618 to 1264 of FIGS. 2A-2C) with a NotI linker. This modified element (shown in FIG. 3A) was inserted into the E. coli vector pTZ18R (Pharmacia), creating a plasmid (pMihsCcw) having a wild-type copy of the C. capitata white (w) gene as a dominant visible marker.

To place the Minos transposase under heat shock control, the left-hand terminal repeat of Minos-2 was replaced by a 456-bp fragment containing the D. melanogaster hsp 70 promoter. This modified element (shown in FIG. 3B) was inserted into the E. coli vector pTZ18R (Pharmacia), creating the transposase-producing plasmid pHSS6hsMi.

The plasmids pMihsCcw and pHSS6hsMi were introduced into pre-blastoderm Medfly w/w embryos by a microinjection procedure similar to that used for Drosophila. For egg collecting, flies were mass-reared in population cages at 24.degree. C. Eggs were collected at 24.degree. C. for 60 minutes, and then were dechorionated, desiccated and microinjected at 18.degree. C. with a mixture of 100 mg/ml helper and 400 mg/ml transposon plasmid DNA as described for Drosophila embryos (Rubin, G. M. and Spradling, A. C., Science, 218:348 (1982)). Modifications of the procedure were not necessary, because the eggs of the two species are similar in morphology and in resistance to desiccation.

A total of 3,998 embryos were injected. After injection, they were left to hatch under halocarbon oil, and first instar larvae were transferred to Petri dishes containing standard larval food (Mintzas, A. C. et al., Dev. Biol., 95:492 (1983)). The 390 adults (G0 generation) resulting from injected embryos were collected within 12 hours after eclosion and back-crossed to w flies in small groups consisting of either 5 G0 males and 10 virgin w females, or 10 G0 females and 5 w males. Fifty-nine such G0 groups were reared in small plastic cages and the G1 progeny were collected and handled separately for each group. To induce expression of the w mini-gene from the Hsp70 promoter, G1 pupae were exposed daily to a 39.degree. C. heat shock for one hour. The 62,510 G1 flies that were produced were screened for the presence of non-white eye phenotypes. As shown in FIG. 4, a total of 72 flies with colored eyes were recovered from four different cages.

The w mini-gene gives partial reversion of the phenotype. Eye color varies in strength among different transformants. The phenotype is dosage-dependent with homozygotes having stronger colors than heterozygotes. These characteristics of w markers are useful in sorting multiple insertions and in distinguishing homozygous from heterozygous transformants. The characteristics are due to low levels of expression combined with chromosomal position effects and have been observed previously in Drosophila.

To establish transformed lines, individual G1's were initially back-crossed to w flies. Single pairs of transformed G2 progeny were then mated, and their homozygous G3 progeny, recognized by their stronger w.sup.+ phenotypes, were used to construct homozygous lines. Table 3 shows the results from the G1 back-crosses. In these crosses, the non-white eye (w.sup.+) phenotype was inherited as a single, dominant trait.

To determine the effect of temperature on the expression of the w mini-gene, a number of G2 pupae were not subjected to the heat shock treatment. When compared to the heat-shocked cohort, G2 flies which had not been heat shocked as pupae showed either paler eye color or no eye color at all; the only exception was lines 3.1 and 3.3, which exhibited an invariant strong yellow eye phenotype. The heat shock dependence clearly showed that the flies (perhaps with the exception of 3.1 and 3.3) were true transformants, rather than revertants of the w mutation.

In cages 3 and 25, differences in the eye color phenotypes of individual G1's from the same cage were detected and bred true, suggesting that independent transformation events had occurred in the same cage.

TABLE 3______________________________________ With heat shock Without heat shock Eye color non- non- Eye color of hetero- white white white white of homo-G1 zygotes eyes eyes eyes eyes zygotes______________________________________1.1 pale yellow 46 53 0 59 apricot1.8 pale yellow 220 274 0 77 apricot1.12 pale yellow 94 69 0 8 apricot3.1 yellow 267 237 110 97 yellow3.3 yellow 225 214 53 49 yellow3.2 pale yellow 132 118 0 76 apricot3.6 pale yellow 70 81 0 81 apricot25.7 pale 119 156 116* 91 apricot apricot25.8 pink 24 18 0 27 peach25.9 pink 30 34 0 9 peach33.2 pale orange 42 50 ND ND orange33.3 pale orange 29 31 ND ND orange33.4 pale orange 16 15 ND ND orange______________________________________ *Eye color much weaker than with heat shock.

To determine the nature of the integration events, DNA from transformants was analyzed by Southern blot hybridizations using several restriction enzymes and two probes (see FIG. 3A), one (M) containing the Minos sequences at the ends of the transposon (which are not present in non-transformed Medfly), and another (W) containing an internal fragment of the w cDNA sequences (which is present in the endogenous w gene).

Adult genomic DNA (approximately 10 .mu.g per lane) was digested with a restriction endonuclease, subjected to agarose gel electrophoresis, blotted onto nitrocellulose membrane filters and hybridized with .sup.32 P-labeled probes. Membranes were pre-hybridized for 6 hours at 65.degree. C. in 7% SDS, 0.5 M phosphate buffer pH 7.4, 1 mM EDTA. Hybridization was for 12-14 hours at 65.degree. C. in 7% SDS, 0.5 M phosphate buffer pH 7.4, 1 mM EDTA. Excess probe was removed by two 10-minute washes with 5% SDS, 40 mM phosphate buffer pH 7.4, 1 mM EDTA at 65.degree. C. followed by a 20-minute wash at room temperature with the same buffer pre-warmed at 65.degree. C.

DNA from lines 3.1, 3.2, 3.3 and 3.6 was cut with SalI and hybridized with a 1 kb HhaI fragment containing Minos sequences present in pMiNot (M probe of FIG. 3A).

DNA from the recipient w strain and from lines 3.1, 3.2, 3.3 and 3.6 was cut with HincII, and probed with a SalI/XhoI fragment containing 1.5 kb of Medfly w cDNA sequences (W probe of FIG. 3A) and with the M probe. Between the two hybridizations the filter was dehybridized by washing with boiling 0.5% SDS solution for 2 minutes.

In Drosophila, insertions of elements like Minos can occur at many different chromosomal sites, and are characterized by precise integration extending through the terminal inverted repeats of the element without transposition of any flanking plasmid DNA. The results of M-hybridized SalI digests document that the events in the Medfly are of the same nature. The transposon has inserted variable host DNA sites, and no significant (>0.2 kb) flanking plasmid DNA to the right of the transposon can be present, because this would have been signaled by the presence of a 2.9 kb band. The results also confirm that two independent events have occurred in cage 3, one represented by lines 3.1 and 3.3 and the other by lines 3.2 and 3.6 (cf. Table 3). These conclusions were also confirmed with HincII digests. Similarly, blots of HincII digests hybridized with the W probe showed the two endogenous w gene bands, plus a third novel band that is characteristic of the insertion event (3.1/3.3 or 3.2/3.6). The shortest band is longer than the 1.9 kb band that would have been expected if the HincII site, 0.2 kb to the right of the Minos end (see FIG. 3A) had been present. The same HincII blot hybridized with the M probe showed that the shortest band is longer than the 1.1 kb band that would have been expected if plasmid sequences to the left of the transposon were present. These results were confirmed with W-hybridized SalI digests.

To assess the integrity of the internal part of the transposon, restriction analysis using EcoRI was performed in three lines derived from cage 25. DNA from strains 25.7, 25.8 and 25.9 was cut with EcoRI and hybridized with the W and M probe sequentially. In addition to the transformants showing non-white eye phenotypes white-eyed siblings (25.9-w, 25.8-w, 25.7-w) were included in this analysis. The results of the hybridization with the W probe indicate that the entire 3.7 kb fragment containing the Hsp70/w marker fusion is present in the w.sup.+ transformants. Hybridization of the same filter with the M probe, which detects "chimeric" end fragments, showed that lines 25.8 and 25.9 contain the same, single insertion of the transposon. The pattern in 25.7 is consistent with the presence of two insertions, neither identical to the 25.8/25.9 event. One of these insertions, defined by the .sup..about. 3 kb and .sup..about. 5.5 kb bands, is also present in the white-eyed siblings of the 25.7 flies. This, presumably, represents a "silent" insertion that does not express the phenotype either due to an undetected lesion in the transposon, or because the transposon has integrated into a silent (perhaps heterochromatic) genomic region.

Restriction analysis of the transformants revealed that, as predicted by the phenotypes (Table 3), two independent transformants were represented among the G1 progeny of cage 3, two in cage 25, and one in cage 33 (Data for transformants from cage 33 are not shown. The restriction patterns of three G1's from cage 1 were identical to these of the 3.2/3.6 event. Evidently, a G0 male present in cage 3 had mated with a G0 female of cage 1, before the G0 flies were sorted into cages.) Only one of these 5 transformants (25.7) had a second (phenotypically silent) event in the same germ line. The different transformants from the same cages are derived either from single or multiple G0 parents. The overall frequency of phenotypically detectable transformation events (5/390 G0 adults) is sufficient for producing several transformants from a single experiment since thousands of embryos can be injected and hundreds of G0 adults can be obtained within a week using a relatively simple experimental setup.

To confirm the presence of a single Minos insertion in transformant 3.1, third instar larva salivary gland polytene chromosomes were prepared and in situ hybridization were performed essentially as described previously (Zacharopoulou, A., et al., Chromosoma, 101:448 (1992)). The 3.7 kb NotI fragment containing the Hsp70/w minigene fusion was used as probe. Hybridization to polytene chromosomes of salivary glands from transformed third instar larvae confirmed the presence of single Minos insertions, allowing their cytological localization.

Those skilled in the art will recognize or be able to ascertain, using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

__________________________________________________________________________# SEQUENCE LISTING- (1) GENERAL INFORMATION:- (iii) NUMBER OF SEQUENCES: 12- (2) INFORMATION FOR SEQ ID NO:1:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 1775 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:- ACGAGCCCCA ACCACTATTA ATTCGAACAG CATGTTTTTT TTGCAGTGCG CA - #ATGTTTAA 60- CACACTATAT TATCAATACT ACTAAAGATA ACACATACCA ATGCATTTCG TC - #TCAAAGAG 120- AATTTTATTC TCTTCACGAC GAAAAAAAAA GTTTTGCTCT ATTTCCAACA AC - #AACAAAAA 180- TATGAGTAAT TTATTCAAAC GGTTTGCTTA AGAGATAAGA AAAAAGTGAC CA - #CTATTAAT 240- TCGAACGCGG CGTAAGCTTA CCTTAATCTC AAGAAGAGCA AAACAAAAGC AA - #CTAATGTA 300- ACGGAATCAT TATCTAGTTA TGATCTGCAA ATAATGTCAC AATACAGCAT GC - #AAAAAAAT 360- TTTAGATTGC TGCAGATCAG TAGAAGTTTA GCAACGATGG TTCGTGGTAA AC - #CTATTTCT 420- AAAGAAATCA GAGTATTGAT TAGGGATTAT TTTAAATCTG GAAAGACACT TA - #CGGAGATA 480- AGCAAGCAAT TAAATTTGCC TAAGTCGTCT GTGCATGGGG TGATACAAAT TT - #TCAAAAAA 540- AATGGGAATA TTGAAAATAA CATTGCGAAT AGAGGCCGAA CATCAGCAAT AA - #CACCCCGC 600- GACAAAAGAC AACTGGCCAA AATTGTTAAG GCTGATCGTC GCCAATCTTT GA - #GAAATTTG 660- GCTTCTAAGT GGTCGCAGCA ATTGGCAAAA CTGTCAAGCG AGAGTGGACG CG - #ACAAATTA 720- AAAAGTATTG GATATGGTTT TTATAAAGTA TGTTTTGTTA TTACCTGTGC AT - #CGTACCCA 780- ATAACTTACT CGTAATCTTA CTCGTAGGCC AAGGAAAAAC CCTTGCTTAC GC - #TTCGTCAA 840- AAAAAGAAGC GTTTGCAATG GGCTCGGGAA AGGATGTCTT GGACTCAAAG GC - #AATAGGAT 900- ACCATCATAT TCAGCGATGA AGCTAAATTT GATGTTAGTG TCGGCGATAC GA - #GAAAACGC 960- GTCATCCGTA AGAGGTCAGA AACATACCAT AAAGACTGCC TTAAAAGAAC AA - #CAAAGTTT1020- CCTGCGAGCA CTATGGTATG GGGATGTATG TCTGCCAAAG GATTAGGAAA AC - #TTCATTTC1080- ATTGAAGGGA CAGTTAATGC TGAAAAATAT ATTAATATTT TACAAGATAG TT - #TGTTGCCA1140- TCAATACCAA AACTATCAGA TTGCGGTGAA TTCACTTTTC AGCAGGACGG AG - #CATCATCG1200- CACACAGCCA AGCGAACCAA AAATTGGCTG CAATATAATC AAATGGAGGT TT - #TAGATTGG1260- CCATCAAATA GTCCAGATCT AAGCCCAATT GAAAATATTT GGTGGCTAAT GA - #AAAACCAG1320- CTTCGAAATG AGCCACAAAG GAATATTTCT GACTTGAAAA TCAAGTTGCA AG - #AGATGTGG1380- GACTCAATTT CTCAAGAGCA TTGCAAAAAT TTGTTAAGCT CAATGCCAAA AC - #GAGTTAAA1440- TGCGTAATGC AGGCCAAGGG CGACGTTACA CAATTCTAAT ATTAATTAAA TT - #ATTGTTTT1500- AAGTATGATA GTAAATCACA TTACGCCGCG TTCGAATTAA TAGTGGTCAC TT - #TTTTCTTA1560- TCTCTTAAGC AAACCGTTTG AATAAATTAC TCATATTTTT GTTGTTGTTG GA - #AATAGAGC1620- AAAACTTTTT TTTTCGTCGT GAAGAGAATA AAATTCTCTT TGAGACGAAA TG - #CATTGGTA1680- TGTGTTATCT TTAGTAGTAT TGATAATATA GTGTGTTAAA CATTGCGCAC TG - #CAAAAAAA1740# 1775 AATA GTGGTTGGGG CTCGT- (2) INFORMATION FOR SEQ ID NO:2:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 1775 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:- ACGAGCCCCA ACCACTATTA ATTCGAACAG CATGTTTTTT TTGCAGTGCG CA - #ATGTTTAA 60- CACACTATAT TATCAATACT ACTAAAGATA ACACATACCA ATGCATTTCG TC - #TCAAAGAG 120- AATTTTATTC TCTTCACGAC GAAAAAAAAA GTTTTGCTCT ATTTCCAACA AC - #AACAAAAA 180- TATGAGTAAT TTATTCAAAC GGTTTGCTTA AGAGATAAGA AAAAAGTGAC CA - #CTATTAAT 240- TCGAACGCGG CGTAAGCTTA CCTTAATCTC AAGAAGAGCA AAACAAAAGC AA - #CTAATGTA 300- ACGGAATCAT TATCTAGTTA TGATCTGCAA ATAATGTCAC AATACAGCAT GC - #AAAAAAAT 360- TTTAGATTGC TGCAGATCAG TAGAAGTTTA GCAACGATGG TTCGTGGTAA AC - #CTATTTCT 420- AAAGAAATCA GAGTATTGAT TAGGGATTAT TTTAAATCTG GAAAGACACT TA - #CGGAGATA 480- AGCAAGCAAT TAAATTTGCC TAAGTCGTCT GTGCATGGGG TGATACAAAT TT - #TCAAAAAA 540- AATGGGAATA TTGAAAATAA CATTGCGAAT AGAGGCCGAA CATCAGCAAT AA - #CACCCCGC 600- GACAAAAGAC AACTGGCCAA AATTGTTAAG GCTGATCGTC GCCAATCTTT GA - #GAAATTTG 660- GCTTCTAAGT GGTCGCAGCA ATTGGCAAAA CTGTCAAGCG AGAGTGGACG CG - #ACAAATTA 720- AAAAGTATTG GATATGGTTT TTATAAAGTA TGTTTTGTTA TTACCTGTGC AT - #CGTACCCA 780- ATAACTTACT CGTAATCTTA CTCGTAGGCC AAGGAAAAAC CCTTGCTTAC GC - #TTCGTCAA 840- AAAAAGAAGC GTTTGCAATG GGCTCGGGAA AGGATGTCTT GGACTCAAAG GC - #AATGGGAT 900- ACCATCATAT TCAGCGATGA AGCTAAATTT GATGTTAGTG TCGGCGATAC GA - #GAAAACGC 960- GTCATCCGTA AGAGGTCAGA AACATACCAT AAAGACTGCC TTAAAAGAAC AA - #CAAAGTTT1020- CCTGCGAGCA CTATGGTATG GGGATGTATG TCTGCCAAAG GATTAGGAAA AC - #TTCATTTC1080- ATTGAAGGGA CAGTTAATGC TGAAAAATAT ATTAATATTT TACAAGATAG TT - #TGTTGCCA1140- TCAATACCAA AACTATTAGA TTGCGGTGAA TTCACTTTTC AGCAGGACGG AG - #CATCATCG1200- CACACAGCCA AGCGAACCAA AAATTGGCTG CAATATAATC AAATGGAGGT TT - #TAGATTGG1260- CCATCAAATA GTCCAGATCT AAGCCCAATT GAAAATATTT GGTGGCTAAT GA - #AAAACCAG1320- CTTCGAAATG AGCCACAAAG GAATATTTCT GACTTGAAAA TCAAGTTGCA AG - #AGATGTGG1380- GACTCAATTT CTCAAGAGCA TTGCAAAAAT TTGTTAAGCT CAATGCCAAA AC - #GAGTTAAA1440- TGCGTAATGC AGGCCAAGGG CGACGTTACA CAATTCTAAT ATTAATTAAA TT - #ATTGTTTT1500- AAGTATGATA GTAAATCACA TTACGCCGCG TTCGAATTAA TAGTGGTCAC TT - #TTTTCTTA1560- TCTCTTAAGC AAACCGTTTG AATAAATTAC TCATATTTTT GTTGTTGTTG GA - #AATAGAGC1620- AAAACTTTTT TTTTCGTCGT GAAGAGAATA AAATTCTCTT TGAGACGAAA TG - #CATTGGTA1680- TGTGTTATCT TTAGTAGTAT TGATAATATA GTGTGTTAAA CATTGCGCAC TG - #CAAAAAAA1740# 1775 AATA GTGGTTGGGG CTCGT- (2) INFORMATION FOR SEQ ID NO:3:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 1775 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:- ACGAGCCCCA ACCACTATTA ATTCGAACAG CATGTTTTTT TTGCAGTGCG CA - #ATGTTTAA 60- CACACTATAT TATCAATACT ACTAAAGATA ACACATACCA ATGCATTTCG TC - #TCAAAGAG 120- AATTTTATTC TCTTCACGAC GAAAAAAAAA GTTTTGCTCT ATTTCCAACA AC - #AACAAAAA 180- TATGAGTAAT TTATTCAAAC GGTTTGCTTA AGAGATAAGA AAAAAGTGAC CA - #CTATTAAT 240- TCGAACGCGG CGTAAGCTTA CCTTAATCTC AAGAAGAGCA AAACAAAAGC AA - #CTAATGTA 300- ACGGAATCAT TATCTAGTTA TGATCTGCAA ATAATGTCAC AATACAGCAT GC - #AAAAAAAT 360- TTTAGATTGC TGCAGATCAG TAGAAGTTTA GCAACGATGG TTCGTGGTAA AC - #CTATTTCT 420- AAAGAAATCA GAGTATTGAT TAGGGATTAT TTTAAATCTG GAAAGACACT TA - #CGGAGATA 480- AGCAAGCAAT TAAATTTGCC TAAGTCGTCT GTGCATGGGG TGATACAAAT TT - #TCAAAAAA 540- AATGGGAATA TTGAAAATAA CATTGCGAAT AGAGGCCGAA CATCAGCAAT AA - #CACCCCGC 600- GACAAAAGAC AACTGGCCAA AATTGTTAAG GCTGATCGTC GCCAATCTTT GA - #GAAATTTG 660- GCTTCTAAGT GGTCGCAGCA ATTGGCAAAA CTGTCAAGCG AGAGTGGACG CG - #ACAAATTA 720- AAAAGTATTG GATATGGTTT TTATAAAGTA TGTTTTGTTA TTACCTGTGC AT - #CGTACCCA 780- ATAACTTACT CGTAATCTTA CTCGTAGGCC AAGGAAAAAC CCTTGCTTAC GC - #TTCGTCAA 840- AAAAAGAAGC GTTTGCAATG GGCTCGGGAA AGGATGTCTT GGACTCAAAG GC - #AATGGGAT 900- ACCATCATAT TCAGCGATGA AGCTAAATTT GATGTTAGTG TCGGCGATAC GA - #GAAAACGC 960- GTCATCCGTA AGAGGTCAGA AACATACCAT AAAGACTGCC TTAAAAGAAC AA - #CAAAGTTT1020- CCTGCGAGCA CTATGGTATG GGGATGTATG TCTGCCAAAG GATTAGGAAA AC - #TTCATTTC1080- ATTGAAGGGA CAGTTAATGC TGAAAAATAT ATTAATATTT TACAAGATAG TT - #TGTTGCCA1140- TCAATACCAA AACTATCAGA TTGCGGTGAA TTCACTTTTC AGCAGGACGG AG - #CATCATCG1200- CACACAGCCA AGCGAACCAA AAATTGGCTG CAATATAATC AAATGGAGGT TT - #TAGATTGG1260- CCATCAAATA GTCCAGATCT AAGCCCAATT GAAAATATTT GGTGGCTAAT GA - #AAAACCAG1320- CTTCGAAATG AGCCACAAAG GAATATTTCT GACTTGAAAA TCAAGTTGCA AG - #AGATGTGG1380- GACTCAATTT CTCAAGAGCA TTGCAAAAAT TTGTTAAGCT CAATGCCAAA AC - #GAGTTAAA1440- TGCGTAATGC AGGCCAAGGG CGACGTTACA CAATTCTAAT ATTAATTAAA TT - #ATTGTTTT1500- AAGTATGATA GTAAATCACA TTACGCCGCG TTCGAATTAA TAGTGGTCAC TT - #TTTTCTTA1560- TCTCTTAAGC AAACCGTTTG AATAAATTAC TCATATTTTT GTTGTTGTTG GA - #AATAGAGC1620- AAAACTTTTT TTTTCGTCGT GAAGAGAATA AAATTCTCTT TGAGACGAAA TG - #CATTGGTA1680- TGTGTTATCT TTAGTAGTAT TGATAATATA GTGTGTTAAA CATTGCGCAC TG - #CAAAAAAA1740# 1775 AATA GTGGTTGGGG CTCGT- (2) INFORMATION FOR SEQ ID NO:4:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 1779 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: join(398..75 - #1, 812..898)- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:- ACGAGCCCCA ACCACTATTA ATTCGAACAG CATGTTTTTT TTGCAGTGCG CA - #ATGTTTAA 60- CACACTATAT TATCAATACT ACTAAAGATA ACACATACCA ATGCATTTCG TC - #TCAAAGAG 120- AATTTTATTC TCTTCACGAC GAAAAAAAAA GTTTTGCTCT ATTTCCAACA AC - #AACAAAAA 180- TATGAGTAAT TTATTCAAAC GGTTTGCTTA AGAGATAAGA AAAAAGTGAC CA - #CTATTAAT 240- TCGAACGCGG CGTAAGCTTA CCTTAATCTC AAGAAGAGCA AAACAAAAGC AA - #CTAATGTA 300- ACGGAATCAT TATCTAGTTA TGATCTGCAA ATAATGTCAC AATACAGCAT GC - #AAAAAAAT 360#GGT AAA CCT 415TCA GTAGAAGTTT AGCAACG ATG GTT CGT# Met Val Arg Gly Lys Pro# 5 1- ATT TCT AAA GAA ATC AGA GTA TTG ATT AGG GA - #T TAT TTT AAA TCT GGA 463Ile Ser Lys Glu Ile Arg Val Leu Ile Arg As - #p Tyr Phe Lys Ser Gly# 20- AAG ACA CTT ACG GAG ATA AGC AAG CAA TTA AA - #T TTG CCT AAG TCG TCT 511Lys Thr Leu Thr Glu Ile Ser Lys Gln Leu As - #n Leu Pro Lys Ser Ser# 35- GTG CAT GGG GTG ATA CAA ATT TTC AAA AAA AA - #T GGG AAT ATT GAA AAT 559Val His Gly Val Ile Gln Ile Phe Lys Lys As - #n Gly Asn Ile Glu Asn# 50- AAC ATT GCG AAT AGA GGC CGA ACA TCA GCA AT - #A ACA CCC CGC GAC AAA 607Asn Ile Ala Asn Arg Gly Arg Thr Ser Ala Il - #e Thr Pro Arg Asp Lys# 70- AGA CAA CTG GCC AAA ATT GTT AAG GCT GAT CG - #T CGC CAA TCT TTG AGA 655Arg Gln Leu Ala Lys Ile Val Lys Ala Asp Ar - #g Arg Gln Ser Leu Arg# 85- AAT TTG GCT TCT AAG TGG TCG CAG ACA ATT GG - #C AAA ACT GTC AAG CGA 703Asn Leu Ala Ser Lys Trp Ser Gln Thr Ile Gl - #y Lys Thr Val Lys Arg# 100- GAG TGG ACG CGA CAG CAA TTA AAA AGT ATT GG - #A TAT GGT TTT TAT AAA 751Glu Trp Thr Arg Gln Gln Leu Lys Ser Ile Gl - #y Tyr Gly Phe Tyr Lys# 115- GTATGTTTTG TTATTACCTG TGCATCGTAC CCAATAACTT ACTCGTAATC TT - #ACTCGTAG 811- GCC AAG GAA AAA CCC TTG CTT ACG CTT CGT CA - #A AAA AAG AAG CGT TTG 859Ala Lys Glu Lys Pro Leu Leu Thr Leu Arg Gl - #n Lys Lys Lys Arg Leu# 130- CAA TGG GCT CGG GAA AGG ATG TCT TGG ACT CA - #A AGG CAA TAGGATACCA 908Gln Trp Ala Arg Glu Arg Met Ser Trp Thr Gl - #n Arg Gln135 1 - #40 1 - #45- TCATATTCAG CGATGAAGCT AAATTTGATG TTAGTGTCGG CGATACGAGA AA - #ACGCGTCA 968- TCCGTAAGAG GTCAGAAACA TACCATAAAG ACTGCCTTAA AAGAACAACA AA - #GTTTCCTG1028- CGAGCACTAT GGTATGGGGA TGTATGTCTG CCAAAGGATT AGGAAAACTT CA - #TTTCATTG1088- AAGGGACAGT TAATGCTGAA AAATATATTA ATATTTTACA AGATAGTTTG TT - #GCCATCAA1148- TACCAAAACT ATCAGATTGC GGTGAATTCA CTTTTCAGCA GGACGGAGCA TC - #ATCGCACA1208- CAGCCAAGCG AACCAAAAAT TGGCTGCAAT ATAATCAAAT GGAGGTTTTA GA - #TTGGCCAT1268- CAAATAGTCC AGATCTAAGC CCAATTGAAA ATATTTGGTG GCTAATGAAA AA - #CCAGCTTC1328- GAAATGAGCC ACAAAGGAAT ATTTCTGACT TGAAAATCAA GTTGCAAGAG AT - #GTGGGACT1388- CAATTTCTCA AGAGCATTGC AAAAATTTGT TAAGCTCAAT GCCAAAACGA GT - #TAAATGCG1448- TAATGCAGGC CAAGGGCGAC GTTACACAAT TCTAATATTA ATTAAATTAT TG - #TTTTAAGT1508- ATGATAGTAA ATCACATTAC GCCGCGTTCG AATTAATAGT GGTCACTTTT TT - #CTTATCTC1568- TTAAGCAAAC CGTTTGAATA AATTACTCAT ATTTTTGTTG TTGTTGGAAA TA - #GAGCAAAA1628- CTTTTTTTTT CGTCGTGAAG AGAATAAAAT TCTCTTTGAG ACGAAATGCA TT - #GGTATGTG1688- TTATCTTTAG TAGTATTGAT AATATAGTGT GTTAAACATT GCGCACTGCA AA - #AAAAACAT1748# 1779 GTGG TTGGGGCTCG T- (2) INFORMATION FOR SEQ ID NO:5:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 147 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:- Met Val Arg Gly Lys Pro Ile Ser Lys Glu Il - #e Arg Val Leu Ile Arg# 15- Asp Tyr Phe Lys Ser Gly Lys Thr Leu Thr Gl - #u Ile Ser Lys Gln Leu# 30- Asn Leu Pro Lys Ser Ser Val His Gly Val Il - #e Gln Ile Phe Lys Lys# 45- Asn Gly Asn Ile Glu Asn Asn Ile Ala Asn Ar - #g Gly Arg Thr Ser Ala# 60- Ile Thr Pro Arg Asp Lys Arg Gln Leu Ala Ly - #s Ile Val Lys Ala Asp# 80- Arg Arg Gln Ser Leu Arg Asn Leu Ala Ser Ly - #s Trp Ser Gln Thr Ile# 95- Gly Lys Thr Val Lys Arg Glu Trp Thr Arg Gl - #n Gln Leu Lys Ser Ile# 110- Gly Tyr Gly Phe Tyr Lys Ala Lys Glu Lys Pr - #o Leu Leu Thr Leu Arg# 125- Gln Lys Lys Lys Arg Leu Gln Trp Ala Arg Gl - #u Arg Met Ser Trp Thr# 140- Gln Arg Gln145- (2) INFORMATION FOR SEQ ID NO:6:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 1779 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: join(398..75 - #1, 812..1480)- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:- ACGAGCCCCA ACCACTATTA ATTCGAACAG CATGTTTTTT TTGCAGTGCG CA - #ATGTTTAA 60- CACACTATAT TATCAATACT ACTAAAGATA ACACATACCA ATGCATTTCG TC - #TCAAAGAG 120- AATTTTATTC TCTTCACGAC GAAAAAAAAA GTTTTGCTCT ATTTCCAACA AC - #AACAAAAA 180- TATGAGTAAT TTATTCAAAC GGTTTGCTTA AGAGATAAGA AAAAAGTGAC CA - #CTATTAAT 240- TCGAACGCGG CGTAAGCTTA CCTTAATCTC AAGAAGAGCA AAACAAAAGC AA - #CTAATGTA 300- ACGGAATCAT TATCTAGTTA TGATCTGCAA ATAATGTCAC AATACAGCAT GC - #AAAAAAAT 360#GGT AAA CCT 415TCA GTAGAAGTTT AGCAACG ATG GTT CGT# Met Val Arg Gly Lys Pro# 5 1- ATT TCT AAA GAA ATC AGA GTA TTG ATT AGG GA - #T TAT TTT AAA TCT GGA 463Ile Ser Lys Glu Ile Arg Val Leu Ile Arg As - #p Tyr Phe Lys Ser Gly# 20- AAG ACA CTT ACG GAG ATA AGC AAG CAA TTA AA - #T TTG CCT AAG TCG TCT 511Lys Thr Leu Thr Glu Ile Ser Lys Gln Leu As - #n Leu Pro Lys Ser Ser# 35- GTG CAT GGG GTG ATA CAA ATT TTC AAA AAA AA - #T GGG AAT ATT GAA AAT 559Val His Gly Val Ile Gln Ile Phe Lys Lys As - #n Gly Asn Ile Glu Asn# 50- AAC ATT GCG AAT AGA GGC CGA ACA TCA GCA AT - #A ACA CCC CGC GAC AAA 607Asn Ile Ala Asn Arg Gly Arg Thr Ser Ala Il - #e Thr Pro Arg Asp Lys# 70- AGA CAA CTG GCC AAA ATT GTT AAG GCT GAT CG - #T CGC CAA TCT TTG AGA 655Arg Gln Leu Ala Lys Ile Val Lys Ala Asp Ar - #g Arg Gln Ser Leu Arg# 85- AAT TTG GCT TCT AAG TGG TCG CAG ACA ATT GG - #C AAA ACT GTC AAG CGA 703Asn Leu Ala Ser Lys Trp Ser Gln Thr Ile Gl - #y Lys Thr Val Lys Arg# 100- GAG TGG ACG CGA CAG CAA TTA AAA AGT ATT GG - #A TAT GGT TTT TAT AAA 751Glu Trp Thr Arg Gln Gln Leu Lys Ser Ile Gl - #y Tyr Gly Phe Tyr Lys# 115- GTATGTTTTG TTATTACCTG TGCATCGTAC CCAATAACTT ACTCGTAATC TT - #ACTCGTAG 811- GCC AAG GAA AAA CCC TTG CTT ACG CTT CGT CA - #A AAA AAG AAG CGT TTG 859Ala Lys Glu Lys Pro Leu Leu Thr Leu Arg Gl - #n Lys Lys Lys Arg Leu# 130- CAA TGG GCT CGG GAA AGG ATG TCT TGG ACT CA - #A AGG CAA TGG GAT ACC 907Gln Trp Ala Arg Glu Arg Met Ser Trp Thr Gl - #n Arg Gln Trp Asp Thr135 1 - #40 1 - #45 1 -#50- ATC ATA TTC AGC GAT GAA GCT AAA TTT GAT GT - #T AGT GTC GGC GAT ACG 955Ile Ile Phe Ser Asp Glu Ala Lys Phe Asp Va - #l Ser Val Gly Asp Thr# 165- AGA AAA CGC GTC ATC CGT AAG AGG TCA GAA AC - #A TAC CAT AAA GAC TGC1003Arg Lys Arg Val Ile Arg Lys Arg Ser Glu Th - #r Tyr His Lys Asp Cys# 180- CTT AAA AGA ACA ACA AAG TTT CCT GCG AGC AC - #T ATG GTA TGG GGA TGT1051Leu Lys Arg Thr Thr Lys Phe Pro Ala Ser Th - #r Met Val Trp Gly Cys# 195- ATG TCT GCC AAA GGA TTA GGA AAA CTT CAT TT - #C ATT GAA GGG ACA GTT1099Met Ser Ala Lys Gly Leu Gly Lys Leu His Ph - #e Ile Glu Gly Thr Val# 210- AAT GCT GAA AAA TAT ATT AAT ATT TTA CAA GA - #T AGT TTG TTG CCA TCA1147Asn Ala Glu Lys Tyr Ile Asn Ile Leu Gln As - #p Ser Leu Leu Pro Ser215 2 - #20 2 - #25 2 -#30- ATA CCA AAA CTA TTA GAT TGC GGT GAA TTC AC - #T TTT CAG CAG GAC GGA1195Ile Pro Lys Leu Leu Asp Cys Gly Glu Phe Th - #r Phe Gln Gln Asp Gly# 245- GCA TCA TCG CAC ACA GCC AAG CGA ACC AAA AA - #T TGG CTG CAA TAT AAT1243Ala Ser Ser His Thr Ala Lys Arg Thr Lys As - #n Trp Leu Gln Tyr Asn# 260- CAA ATG GAG GTT TTA GAT TGG CCA TCA AAT AG - #T CCA GAT CTA AGC CCA1291Gln Met Glu Val Leu Asp Trp Pro Ser Asn Se - #r Pro Asp Leu Ser Pro# 275- ATT GAA AAT ATT TGG TGG CTA ATG AAA AAC CA - #G CTT CGA AAT GAG CCA1339Ile Glu Asn Ile Trp Trp Leu Met Lys Asn Gl - #n Leu Arg Asn Glu Pro# 290- CAA AGG AAT ATT TCT GAC TTG AAA ATC AAG TT - #G CAA GAG ATG TGG GAC1387Gln Arg Asn Ile Ser Asp Leu Lys Ile Lys Le - #u Gln Glu Met Trp Asp295 3 - #00 3 - #05 3 -#10- TCA ATT TCT CAA GAG CAT TGC AAA AAT TTG TT - #A AGC TCA ATG CCA AAA1435Ser Ile Ser Gln Glu His Cys Lys Asn Leu Le - #u Ser Ser Met Pro Lys# 325- CGA GTT AAA TGC GTA ATG CAG GCC AAG GGC GA - #C GTT ACA CAA TTC1480Arg Val Lys Cys Val Met Gln Ala Lys Gly As - #p Val Thr Gln Phe# 340- TAATATTAAT TAAATTATTG TTTTAAGTAT GATAGTAAAT CACATTACGC CG - #CGTTCGAA1540- TTAATAGTGG TCACTTTTTT CTTATCTCTT AAGCAAACCG TTTGAATAAA TT - #ACTCATAT1600- TTTTGTTGTT GTTGGAAATA GAGCAAAACT TTTTTTTTCG TCGTGAAGAG AA - #TAAAATTC1660- TCTTTGAGAC GAAATGCATT GGTATGTGTT ATCTTTAGTA GTATTGATAA TA - #TAGTGTGT1720- TAAACATTGC GCACTGCAAA AAAAACATGC TGTTCGAATT AATAGTGGTT GG - #GGCTCGT1779- (2) INFORMATION FOR SEQ ID NO:7:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 341 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:- Met Val Arg Gly Lys Pro Ile Ser Lys Glu Il - #e Arg Val Leu Ile Arg# 15- Asp Tyr Phe Lys Ser Gly Lys Thr Leu Thr Gl - #u Ile Ser Lys Gln Leu# 30- Asn Leu Pro Lys Ser Ser Val His Gly Val Il - #e Gln Ile Phe Lys Lys# 45- Asn Gly Asn Ile Glu Asn Asn Ile Ala Asn Ar - #g Gly Arg Thr Ser Ala# 60- Ile Thr Pro Arg Asp Lys Arg Gln Leu Ala Ly - #s Ile Val Lys Ala Asp# 80- Arg Arg Gln Ser Leu Arg Asn Leu Ala Ser Ly - #s Trp Ser Gln Thr Ile# 95- Gly Lys Thr Val Lys Arg Glu Trp Thr Arg Gl - #n Gln Leu Lys Ser Ile# 110- Gly Tyr Gly Phe Tyr Lys Ala Lys Glu Lys Pr - #o Leu Leu Thr Leu Arg# 125- Gln Lys Lys Lys Arg Leu Gln Trp Ala Arg Gl - #u Arg Met Ser Trp Thr# 140- Gln Arg Gln Trp Asp Thr Ile Ile Phe Ser As - #p Glu Ala Lys Phe Asp145 1 - #50 1 - #55 1 -#60- Val Ser Val Gly Asp Thr Arg Lys Arg Val Il - #e Arg Lys Arg Ser Glu# 175- Thr Tyr His Lys Asp Cys Leu Lys Arg Thr Th - #r Lys Phe Pro Ala Ser# 190- Thr Met Val Trp Gly Cys Met Ser Ala Lys Gl - #y Leu Gly Lys Leu His# 205- Phe Ile Glu Gly Thr Val Asn Ala Glu Lys Ty - #r Ile Asn Ile Leu Gln# 220- Asp Ser Leu Leu Pro Ser Ile Pro Lys Leu Le - #u Asp Cys Gly Glu Phe225 2 - #30 2 - #35 2 -#40- Thr Phe Gln Gln Asp Gly Ala Ser Ser His Th - #r Ala Lys Arg Thr Lys# 255- Asn Trp Leu Gln Tyr Asn Gln Met Glu Val Le - #u Asp Trp Pro Ser Asn# 270- Ser Pro Asp Leu Ser Pro Ile Glu Asn Ile Tr - #p Trp Leu Met Lys Asn# 285- Gln Leu Arg Asn Glu Pro Gln Arg Asn Ile Se - #r Asp Leu Lys Ile Lys# 300- Leu Gln Glu Met Trp Asp Ser Ile Ser Gln Gl - #u His Cys Lys Asn Leu305 3 - #10 3 - #15 3 -#20- Leu Ser Ser Met Pro Lys Arg Val Lys Cys Va - #l Met Gln Ala Lys Gly# 335- Asp Val Thr Gln Phe 340- (2) INFORMATION FOR SEQ ID NO:8:- (i) SEQUENCE CHARACTERISTICS:#pairs (A) LENGTH: 1779 base (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: DNA (genomic)- (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: join(398..75 - #1, 812..1480)- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:- ACGAGCCCCA ACCACTATTA ATTCGAACAG CATGTTTTTT TTGCAGTGCG CA - #ATGTTTAA 60- CACACTATAT TATCAATACT ACTAAAGATA ACACATACCA ATGCATTTCG TC - #TCAAAGAG 120- AATTTTATTC TCTTCACGAC GAAAAAAAAA GTTTTGCTCT ATTTCCAACA AC - #AACAAAAA 180- TATGAGTAAT TTATTCAAAC GGTTTGCTTA AGAGATAAGA AAAAAGTGAC CA - #CTATTAAT 240- TCGAACGCGG CGTAAGCTTA CCTTAATCTC AAGAAGAGCA AAACAAAAGC AA - #CTAATGTA 300- ACGGAATCAT TATCTAGTTA TGATCTGCAA ATAATGTCAC AATACAGCAT GC - #AAAAAAAT 360#GGT AAA CCT 415TCA GTAGAAGTTT AGCAACG ATG GTT CGT# Met Val Arg Gly Lys Pro# 5 1- ATT TCT AAA GAA ATC AGA GTA TTG ATT AGG GA - #T TAT TTT AAA TCT GGA 463Ile Ser Lys Glu Ile Arg Val Leu Ile Arg As - #p Tyr Phe Lys Ser Gly# 20- AAG ACA CTT ACG GAG ATA AGC AAG CAA TTA AA - #T TTG CCT AAG TCG TCT 511Lys Thr Leu Thr Glu Ile Ser Lys Gln Leu As - #n Leu Pro Lys Ser Ser# 35- GTG CAT GGG GTG ATA CAA ATT TTC AAA AAA AA - #T GGG AAT ATT GAA AAT 559Val His Gly Val Ile Gln Ile Phe Lys Lys As - #n Gly Asn Ile Glu Asn# 50- AAC ATT GCG AAT AGA GGC CGA ACA TCA GCA AT - #A ACA CCC CGC GAC AAA 607Asn Ile Ala Asn Arg Gly Arg Thr Ser Ala Il - #e Thr Pro Arg Asp Lys# 70- AGA CAA CTG GCC AAA ATT GTT AAG GCT GAT CG - #T CGC CAA TCT TTG AGA 655Arg Gln Leu Ala Lys Ile Val Lys Ala Asp Ar - #g Arg Gln Ser Leu Arg# 85- AAT TTG GCT TCT AAG TGG TCG CAG ACA ATT GG - #C AAA ACT GTC AAG CGA 703Asn Leu Ala Ser Lys Trp Ser Gln Thr Ile Gl - #y Lys Thr Val Lys Arg# 100- GAG TGG ACG CGA CAG CAA TTA AAA AGT ATT GG - #A TAT GGT TTT TAT AAA 751Glu Trp Thr Arg Gln Gln Leu Lys Ser Ile Gl - #y Tyr Gly Phe Tyr Lys# 115- GTATGTTTTG TTATTACCTG TGCATCGTAC CCAATAACTT ACTCGTAATC TT - #ACTCGTAG 811- GCC AAG GAA AAA CCC TTG CTT ACG CTT CGT CA - #A AAA AAG AAG CGT TTG 859Ala Lys Glu Lys Pro Leu Leu Thr Leu Arg Gl - #n Lys Lys Lys Arg Leu# 130- CAA TGG GCT CGG GAA AGG ATG TCT TGG ACT CA - #A AGG CAA TGG GAT ACC 907Gln Trp Ala Arg Glu Arg Met Ser Trp Thr Gl - #n Arg Gln Trp Asp Thr135 1 - #40 1 - #45 1 -#50- ATC ATA TTC AGC GAT GAA GCT AAA TTT GAT GT - #T AGT GTC GGC GAT ACG 955Ile Ile Phe Ser Asp Glu Ala Lys Phe Asp Va - #l Ser Val Gly Asp Thr# 165- AGA AAA CGC GTC ATC CGT AAG AGG TCA GAA AC - #A TAC CAT AAA GAC TGC1003Arg Lys Arg Val Ile Arg Lys Arg Ser Glu Th - #r Tyr His Lys Asp Cys# 180- CTT AAA AGA ACA ACA AAG TTT CCT GCG AGC AC - #T ATG GTA TGG GGA TGT1051Leu Lys Arg Thr Thr Lys Phe Pro Ala Ser Th - #r Met Val Trp Gly Cys# 195- ATG TCT GCC AAA GGA TTA GGA AAA CTT CAT TT - #C ATT GAA GGG ACA GTT1099Met Ser Ala Lys Gly Leu Gly Lys Leu His Ph - #e Ile Glu Gly Thr Val# 210- AAT GCT GAA AAA TAT ATT AAT ATT TTA CAA GA - #T AGT TTG TTG CCA TCA1147Asn Ala Glu Lys Tyr Ile Asn Ile Leu Gln As - #p Ser Leu Leu Pro Ser215 2 - #20 2 - #25 2 -#30- ATA CCA AAA CTA TCA GAT TGC GGT GAA TTC AC - #T TTT CAG CAG GAC GGA1195Ile Pro Lys Leu Ser Asp Cys Gly Glu Phe Th - #r Phe Gln Gln Asp Gly# 245- GCA TCA TCG CAC ACA GCC AAG CGA ACC AAA AA - #T TGG CTG CAA TAT AAT1243Ala Ser Ser His Thr Ala Lys Arg Thr Lys As - #n Trp Leu Gln Tyr Asn# 260- CAA ATG GAG GTT TTA GAT TGG CCA TCA AAT AG - #T CCA GAT CTA AGC CCA1291Gln Met Glu Val Leu Asp Trp Pro Ser Asn Se - #r Pro Asp Leu Ser Pro# 275- ATT GAA AAT ATT TGG TGG CTA ATG AAA AAC CA - #G CTT CGA AAT GAG CCA1339Ile Glu Asn Ile Trp Trp Leu Met Lys Asn Gl - #n Leu Arg Asn Glu Pro# 290- CAA AGG AAT ATT TCT GAC TTG AAA ATC AAG TT - #G CAA GAG ATG TGG GAC1387Gln Arg Asn Ile Ser Asp Leu Lys Ile Lys Le - #u Gln Glu Met Trp Asp295 3 - #00 3 - #05 3 -#10- TCA ATT TCT CAA GAG CAT TGC AAA AAT TTG TT - #A AGC TCA ATG CCA AAA1435Ser Ile Ser Gln Glu His Cys Lys Asn Leu Le - #u Ser Ser Met Pro Lys# 325- CGA GTT AAA TGC GTA ATG CAG GCC AAG GGC GA - #C GTT ACA CAA TTC1480Arg Val Lys Cys Val Met Gln Ala Lys Gly As - #p Val Thr Gln Phe# 340- TAATATTAAT TAAATTATTG TTTTAAGTAT GATAGTAAAT CACATTACGC CG - #CGTTCGAA1540- TTAATAGTGG TCACTTTTTT CTTATCTCTT AAGCAAACCG TTTGAATAAA TT - #ACTCATAT1600- TTTTGTTGTT GTTGGAAATA GAGCAAAACT TTTTTTTTCG TCGTGAAGAG AA - #TAAAATTC1660- TCTTTGAGAC GAAATGCATT GGTATGTGTT ATCTTTAGTA GTATTGATAA TA - #TAGTGTGT1720- TAAACATTGC GCACTGCAAA AAAAACATGC TGTTCGAATT AATAGTGGTT GG - #GGCTCGT1779- (2) INFORMATION FOR SEQ ID NO:9:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 341 amino (B) TYPE: amino acid (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: protein- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:- Met Val Arg Gly Lys Pro Ile Ser Lys Glu Il - #e Arg Val Leu Ile Arg# 15- Asp Tyr Phe Lys Ser Gly Lys Thr Leu Thr Gl - #u Ile Ser Lys Gln Leu# 30- Asn Leu Pro Lys Ser Ser Val His Gly Val Il - #e Gln Ile Phe Lys Lys# 45- Asn Gly Asn Ile Glu Asn Asn Ile Ala Asn Ar - #g Gly Arg Thr Ser Ala# 60- Ile Thr Pro Arg Asp Lys Arg Gln Leu Ala Ly - #s Ile Val Lys Ala Asp# 80- Arg Arg Gln Ser Leu Arg Asn Leu Ala Ser Ly - #s Trp Ser Gln Thr Ile# 95- Gly Lys Thr Val Lys Arg Glu Trp Thr Arg Gl - #n Gln Leu Lys Ser Ile# 110- Gly Tyr Gly Phe Tyr Lys Ala Lys Glu Lys Pr - #o Leu Leu Thr Leu Arg# 125- Gln Lys Lys Lys Arg Leu Gln Trp Ala Arg Gl - #u Arg Met Ser Trp Thr# 140- Gln Arg Gln Trp Asp Thr Ile Ile Phe Ser As - #p Glu Ala Lys Phe Asp145 1 - #50 1 - #55 1 -#60- Val Ser Val Gly Asp Thr Arg Lys Arg Val Il - #e Arg Lys Arg Ser Glu# 175- Thr Tyr His Lys Asp Cys Leu Lys Arg Thr Th - #r Lys Phe Pro Ala Ser# 190- Thr Met Val Trp Gly Cys Met Ser Ala Lys Gl - #y Leu Gly Lys Leu His# 205- Phe Ile Glu Gly Thr Val Asn Ala Glu Lys Ty - #r Ile Asn Ile Leu Gln# 220- Asp Ser Leu Leu Pro Ser Ile Pro Lys Leu Se - #r Asp Cys Gly Glu Phe225 2 - #30 2 - #35 2 -#40- Thr Phe Gln Gln Asp Gly Ala Ser Ser His Th - #r Ala Lys Arg Thr Lys# 255- Asn Trp Leu Gln Tyr Asn Gln Met Glu Val Le - #u Asp Trp Pro Ser Asn# 270- Ser Pro Asp Leu Ser Pro Ile Glu Asn Ile Tr - #p Trp Leu Met Lys Asn# 285- Gln Leu Arg Asn Glu Pro Gln Arg Asn Ile Se - #r Asp Leu Lys Ile Lys# 300- Leu Gln Glu Met Trp Asp Ser Ile Ser Gln Gl - #u His Cys Lys Asn Leu305 3 - #10 3 - #15 3 -#20- Leu Ser Ser Met Pro Lys Arg Val Lys Cys Va - #l Met Gln Ala Lys Gly# 335- Asp Val Thr Gln Phe 340- (2) INFORMATION FOR SEQ ID NO:10:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 5 amino (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: peptide- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:- Met Val Trp Gly Cys1 5- (2) INFORMATION FOR SEQ ID NO:11:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 8 amino (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: peptide- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:- Trp Pro Ser Gln Ser Pro Asp Leu1 5- (2) INFORMATION FOR SEQ ID NO:12:- (i) SEQUENCE CHARACTERISTICS:#acids (A) LENGTH: 8 amino (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear- (ii) MOLECULE TYPE: peptide- (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:- Trp Pro Ser Asn Ser Pro Asp Leu1 5__________________________________________________________________________

Claims

1. A purified transposase protein, or peptide fragments therof having transposase activity encoded by a DNA sequence characterized by the ability to hybridize in a buffered solution of 0.9 M NaCl, at a temperature of 55.degree. C., to DNA having a sequence selected from the group consisting of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8.

2. The purified transposase protein of claim 1 encoded by a DNA sequence selected from the group consisting of: SEQ ID NO:6 and SEQ ID NO:8.

3. A purified transposase protein of claim 2 comprising a protein of about 341 amino acids.

4. A purified transposase protein having an amino acid sequence selected from the group consisting of: SEQ ID NO:7 and SEQ ID NO:9.