Patent Grant
10730048
The present invention relates to droplet microfluidic devices, and more specifically to Active Matrix Electro-wetting-On-Dielectric (AM-EWOD) devices, including structures and control methods for enhanced loading of fluid into such devices.
Electrowetting on dielectric (EWOD) is a well-known technique for manipulating droplets of fluid by the application of an electric field. Active Matrix EWOD (AM-EWOD) refers to implementation of EWOD in an active matrix array incorporating transistors, for example by using thin film transistors (TFTs). It is thus a candidate technology for digital microfluidics for lab-on-a-chip technology. An introduction to the basic principles of the technology can be found in “Digital microfluidics: is a true lab-on-a-chip possible?”, R. B. Fair, Microfluid. Nanofluid. (2007) 3:245-281).
On the top substrate 16 is a second hydrophobic coating 28 with which the liquid droplet 14 may come into contact. Interposed between the top substrate 16 and the second hydrophobic coating 28 is a reference electrode 30.
The contact angle θ is defined as shown in
In operation, voltages termed the EW drive voltages, (e.g. VT, V0 and V00 in
Example configurations and operation of EWOD devices are described in the following. U.S. Pat. No. 6,911,132 (Pamula et al., issued Jun. 28, 2005) discloses a two dimensional EWOD array to control the position and movement of droplets in two dimensions. U.S. Pat. No. 6,565,727 (Shenderov, issued May 20, 2003) further discloses methods for other droplet operations including the splitting and merging of droplets, and the mixing together of droplets of different materials. U.S. Pat. No. 7,163,612 (Sterling et al., issued Jan. 16, 2007) describes how TFT based thin film electronics may be used to control the addressing of voltage pulses to an EWOD array by using circuit arrangements very similar to those employed in AM display technologies.
The approach of U.S. Pat. No. 7,163,612 may be termed “Active Matrix Electrowetting on Dielectric” (AM-EWOD). There are several advantages in using TFT based thin film electronics to control an EWOD array, namely:
As described above with respect to the representative EWOD structure, the EWOD channel or gap defined by the two substrates initially is filled with the non-polar fluid (oil). The liquid droplets 14 including a polar material, i.e., the droplets to be manipulated by operation of the EWOD device, must be inputted from a fluid reservoir into the EWOD channel or gap. As the fluid from the reservoir for the droplets in inputted, oil gets displaced and is removed from the EWOD channel.
Different mechanisms have been devised for the inputting or loading of polar fluid for the liquid droplets into such devices. For example, U.S. Pat. No. 8,702,938 (Srinivasan et al., issued Apr. 22, 2014) describes an EWOD device with a plastic frame surrounding a glass substrate. The plastic frame contains holes which provide a fluid path from the exterior of a droplet actuator and into the EWOD channel or gap. US20100282609 (Pollack et al., published Nov. 11, 2010) describes the use of a piston mechanism to force fluid into reservoirs contained in a device already loaded with oil. US20100032293 (Pollack et al., published Feb. 11, 2010) describes a filling mechanism where fluid flows onto the array containing oil via a variable pressure source, and droplets of fluid are held on the array and the excess fluid is drained. US20130161193 (Jacobs et al., published Jun. 27, 2013) describes a method to drive fluid into a device filled with oil by using, for example, a bistable actuator. Mechanisms for precise control of the input of fluid into the EWOD channel or gap are not taught in these patent documents.
U.S. Pat. No. 9,630,180 (Srinivasan et al., issued Apr. 25, 2017) describes controlling the hydrostatic head of a droplet actuator to avoid fluid spontaneously flowing into the gap between the bottom and top substrates. The patent illustrates schematics of potential droplet actuators and associated electrode patterns for drawing fluid out of such a droplet actuator. In addition, this patent describes having a waste reservoir which is made as large as possible to lower the pressure at the reservoir. As representative, the teachings include restrictions on the relative sizes of different parts of the droplet actuator.
WO 2017/047082 (Walton et al., published Mar. 23, 2017) describes partially filling a device with a metered volume of oil. Fluid is then input into the device easily by venting air. An exemplary embodiment includes filling the device with oil, adding fluid to the input ports, then extracting a sufficient volume of oil to enable at least some of the fluid to enter the device. With the disclosed device configurations, there is a limit to the quantity of fluid that can be processed by such a device, and the loading of fluid occurs with the user manually removing a portion of the oil.
The manner and efficiency of the input of the polar fluid for the liquid droplets can affect the overall performance of the EWOD device. The present invention relates to EWOD and AM-EWOD microfluidic devices, and to controlling the input and output of fluids to and from the hydrophobic EWOD channel defined between the substrate structures of such devices. The described configurations are of particular relevance to devices where the total volume of fluid which needs to be processed through the device exceeds that which can be handled within the EWOD channel at any one time, such as may occur over multiple droplet manipulation operations or complex reaction protocols. Because the total processed fluid exceeds that which can be handled within the EWOD channel at any one time, in conventional configurations overfilling can occur which can undermine device operation as excess oil spills over onto the external portions of the device. On the other hand, fluid needs to be inputted, processed and then removed in an efficient manner, so that a next portion of fluid can be inputted and processed to maintain a desired throughput.
Efficiency with high-throughput versus the avoidance of over-filling presents a delicate balance for device operation that is not adequately addressed by conventional configurations. For example, none of the patent documents referenced above recognizes the potential for over filling the device with oil as fluid for the droplets is loaded, and the patent documents do not address efficient ways to extract fluid of the droplets from the device to ready the device for input of a subsequent portion of polar fluid for droplets to be processed. Without precise control of fluid input and extraction, a high throughput without over filling is not achieved.
As referenced above, the fluids that are introduced to form the liquid droplets for manipulation are polar. As such, in the absence of an electrowetting voltage, such fluids tend to repel from the hydrophobic surfaces that define the EWOD channel. While the electrowetting voltage is actuated, such surfaces become hydrophilic to better accept the polar fluid, but filling under action of the electrowetting voltage itself is not readily controlled. Accordingly, in conventional systems pipetting the fluid into the EWOD channel may be employed, but upon removal of the pipette an undesirable backflow of the fluid out from the EWOD channel can occur, and such input method also does not address any potential for overfilling.
Accordingly, the present disclosure relates to an EWOD device configuration having structures that perform fluid input and extraction in a controlled and automated manner, with the use of a seal mechanism to initiate the fluid input. Aspects of the device include an EWOD device structure and fluid input methods whereby an input fluid can be “held back” outside the channel in a dedicated fluid input well, and then input into the channel at the desired time in a controlled and reproducible manner through the manipulation of the seal mechanism of a control port. In exemplary embodiments, fluid may be retained in a fluid input well, which may be a plastic fluid holding well, prior to input into the EWIOD channel. The structure of the device may include at least one fluid input port for inputting the fluid, and at least one control port for controlling such input, the control port having a removable seal. A vent hole also may be provided through the top substrate assembly for the elimination of air from the EWOD channel. The seal is initially in a sealing position to seal the control port during a fluid holding state, and the seal is removed from the sealing position to unseal the control port to initiate a fluid input state.
An exemplary method for controlling fluid input into the EWOD channel of the EWOD device may include the following steps. First, oil is input into the EWOD channel via at least one of the fluid input ports. The fluid control port does not fill with oil due to the presence of the seal, and any excess oil fills the fluid input wells of the input ports. In addition, oil is held back within the vent hole in the top substrate due to surface tension, i.e., the oil does not spill over the top substrate and generates a requisite hydrostatic pressure for inputting fluid into the device. Second, a polar fluid reservoir used for forming the liquid droplets is input into a fluid input port, and is maintained within an input well defined by the fluid input port during a fluid holding state. Because of the presence of the seal, fluid from the fluid reservoir substantially does not enter the EWOD channel during the fluid holding state at this point even if an electrowetting force is applied to the fluid in the input well. Third, a fluid input state is initiated by removing the seal from control port, either by actually removing the seal or at least piercing the seal to render the control port in an unsealed state. Accordingly, filling is initiated by removing or piercing the seal on the control port, and this results in an automatic internal displacement of oil to fill a control well defined by the control port. Fourth, the electrowetting mechanism is used to dispense droplets from the input fluid well into the EWOD channel in a controlled manner under software control executed by the EWOD device control system. There may be multiple fluid input ports and corresponding input wells from which fluid may be inputted into the EWOD channel. The total volume of fluid that can enter the EWOD channel, comprised of any suitable number of droplets input from any of the fluid input wells, generally is determined by the quantity of oil displaced from the channel by the removal or piercing of the control port seal.
In exemplary embodiments, when it is desired to input an additional volume of fluid that exceeds a maximum that can be handled within the EWOD channel at any one time, the processed fluid is extracted to ready the device for input of additional fluid into the EWOD channel. This may be accomplished by performing the following additional steps, and multiple cycles of input/droplet manipulation/extraction may be repeated. First, one or more droplets are moved to an exit port, which may be part of the control port, by electrowetting. Second, the electrowetting is deactivated in the region of the exit port, and the fluid leaves the EWOD channel and enters the control well from through the exit port under the hydrostatic pressure. Additional fluid can now be input into the EWOD channel through one or more of the input fluid ports using the electrowetting mechanism, by exploiting the volume displaced within the EWOD channel by the extraction of fluid at the exit port.
In exemplary embodiments, therefore, an electrowetting on dielectric (EWOD) device includes a first substrate assembly and a second substrate assembly spaced apart to define a channel between the first and second substrate assemblies; an input port in fluid communication with the channel, the input port defining an input well for receiving a fluid for inputting into the channel; and a control port in fluid communication with the channel, the control port defining a control well for receiving a fluid and having a seal that seals the control port in a sealed state in which fluid is restricted from entering the control well from the channel. When the seal is removed or pierced, the control port is placed in an unsealed state thereby permitting fluid to enter the control well from the channel.
Another aspect of the invention is an enhanced method of controlling fluid flow through an EWOD device using such an EWOD device with a sealed control port. The method may include the steps of inputting a non-polar fluid into the channel via the input port, wherein excess fluid after filling the EWOD channel accumulates within the input well and is restricted from entering the control well by the seal; inputting a polar fluid into the input well to form a fluid reservoir at a channel port that fluidly connects the input well to the channel; and removing or piercing the seal to place the control port in an unsealed state, wherein a portion of the non-polar fluid within the channel is displaced from the channel into the control well, and a portion of the polar fluid of the fluid reservoir enters the channel from the input well. After the seal is removed or pierced, an electrowetting force may be applied to dispense one or more liquid droplets from the fluid reservoir for manipulation within the channel. To remove dispensed droplets from the channel, the method further may include applying the electrowetting force to move the dispensed droplets to an exit port in fluid communication with the EWOD channel; forming an exit reservoir of the moved dispensed droplets adjacent to the exit port; and deactivating the electrowetting force in a region of the exit port, wherein the exit reservoir exits the channel via the exit port under a hydrostatic force. Multiple cycles of fluid input/droplet manipulation/fluid extraction may be repeated to perform complex reaction protocols.
The present invention has advantages over conventional filling mechanisms such as those described in the background section. For example, U.S. Pat. No. 9,630,180 uses the hydrostatic head of pressure of the droplet actuator to allow fluid into the EWOD channel between the bottom and top substrates. Additionally, U.S. Pat. No. 9,630,180 refers to a ‘dead volume’—the volume of fluid which remains in the well and cannot be brought onto the device. An advantage of the present invention is that the dead volume is smaller. U.S. Pat. No. 9,630,180 also refers to an activation volume, as the minimum volume of fluid required to generate a sufficient head of pressure. A further advantage of the present invention is that the activation volume can be smaller since the required difference in the head of pressure is generated be means of the seal. This invention is thus able to make more efficient use of fluid input into the well.
Such device does not use a seal to create a large difference in hydrostatic pressure, and there is presumably a limit to the quantity of fluid that can be drawn on in this manner. WO 2017/047082 describes filling the device with oil, adding fluid to the input ports, and then then extracting a sufficient volume of oil to enable at least some of the fluid to enter the device. There is a limit to the quantity of fluid which can be processed by such a device, as it is determined by the amount of oil being extracted. This patent document does not recognize the potential for over filling the device, and does not provide for any automated fluid input. Accordingly, the conventional configurations cannot remove oil and then load polar fluid for droplets into the EWOD channel, and then extract fluid from the device for subsequent input of additional polar fluid for droplets, in a controlled and automated manner with the use of a seal to initiate the fluid input.
These and further features of the present invention will be apparent with reference to the following description and attached drawings. In the description and drawings, particular embodiments of the invention have been disclosed in detail as being indicative of some of the ways in which the principles of the invention may be employed, but it is understood that the invention is not limited correspondingly in scope. Rather, the invention includes all changes, modifications and equivalents coming within the spirit and terms of the claims appended hereto. Features that are described and/or illustrated with respect to one embodiment may be used in the same way or in a similar way in one or more other embodiments and/or in combination with or instead of the features of the other embodiments.
Embodiments of the present invention will now be described with reference to the drawings, wherein like reference numerals are used to refer to like elements throughout. It will be understood that the figures are not necessarily to scale.
The microfluidic system further may include a control system configured to control actuation voltages applied to the electrode array of the microfluidic device to perform manipulation operations to the fluid droplets. For example, the reader 32 may contain such a control system configured as control electronics 38 and a storage device 40 that may store any application software any data associated with the system. The control electronics 38 may include suitable circuitry and/or processing devices that are configured to carry out various control operations relating to control of the AM-EWOD device 36, such as a CPU, microcontroller or microprocessor.
Among their functions, the control electronics may comprise a part of the overall control system that may execute program code embodied as a control application within the storage device 40. It will be apparent to a person having ordinary skill in the art of computer programming, and specifically in application programming for electronic control devices, how to program the control system to operate and carry out logical functions associated with the stored control application. Accordingly, details as to specific programming code have been left out for the sake of brevity. The storage device 40 may be configured as a non-transitory computer readable medium, such as random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or Flash memory), or any other suitable medium. Also, while the code may be executed by control electronics 38 in accordance with an exemplary embodiment, such control system functionality could also be carried out via dedicated hardware, firmware, software, or combinations thereof, without departing from the scope of the invention.
The control system may be configured to perform some or all of the following functions:
In the example of
The control system, such as via the control electronics 38, may supply and control the actuation electrowetting voltages applied to the electrode array of the microfluidics device 36, such as required voltage and timing signals to perform droplet manipulation operations and sense liquid droplets on the AM-EWOD device 36. The control electronics further may execute the application software to generate and output control voltages for droplet sensing and performing sensing operations. The reader 32 and cartridge 34 may be electrically connected together while in use, for example by a cable of connecting wires 42, although various other methods (e.g. wireless connection) of providing electrical communication may be used as are known to those of ordinary skill in the art.
Also similarly to the conventional structure of
For the purposes of driving and sensing the array elements, the electrical load 70A/70B overall functions in effect as a capacitor, whose value depends on whether a liquid droplet 52 is present or not at a given element electrode 48. In the case where a droplet is present, the capacitance is relatively high (typically of order pico-Farads), whereas if there is no liquid droplet present the capacitance is low (typically of order femto-Farads). If a droplet partially covers a given electrode 48 then the capacitance may approximately represent the extent of coverage of the element electrode 48 by the liquid droplet 52.
A serial interface 82 may also be provided to process a serial input data stream and facilitate the programming of the required voltages to the element electrodes 48 in the array 50. A voltage supply interface 84 provides the corresponding supply voltages, top substrate drive voltages, and other requisite voltage inputs as further described herein. A number of connecting wires 86 between the lower substrate 44 and external control electronics, power supplies and any other components can be made relatively few, even for large array sizes. Optionally, the serial data input may be partially parallelized. For example, if two data input lines are used the first may supply data for columns 1 to X/2, and the second for columns (1+X/2) to M with minor modifications to the column driver circuits 76. In this way the rate at which data can be programmed to the array is increased, which is a standard technique used in Liquid Crystal Display driving circuitry.
Generally, an exemplary AM-EWOD device 36 that includes thin film electronics 46 may be configured as follows. The AM-EWOD device 36 includes the reference electrode 58 mentioned above (which, optionally, could be an in-plane reference electrode) and a plurality of individual array elements 51 on the array of elements 50, each array element 51 including an array element electrode 48 and array element circuitry 72. Relatedly, the AM-EWOD device 36 may be configured to perform a method of actuating the array elements to manipulate liquid droplets on the array by controlling an electro-wetting voltage to be applied to a plurality of array elements to generate an electrowetting force. The applied voltages may be provided by operation of the control system described as to
The array element circuit 72 may typically perform the functions of:
Exemplary configurations of array element circuits 72 including impedance sensor circuitry are known in the art, and for example are described in detail in U.S. Pat. No. 8,653,832 referenced in the background art section, and commonly assigned UK application GB1500261.1, both of which are incorporated here by reference. These patent documents include descriptions of how the droplet may be actuated (by means of electro-wetting) and how the droplet may be sensed by capacitive or impedance sensing means. Typically, capacitive and impedance sensing may be analogue and may be performed simultaneously, or near simultaneously, at every element in the array. By processing the returned information from such a sensor (for example in the application software in the storage device 40 of the reader 32), the control system described above can determine in real-time, or almost real-time the position, size, centroid and perimeter of each liquid droplet present in the array of elements 50. As referenced in connection with
The present invention provides enhanced control of the input of fluids into the EWOD device, including a non-polar fluid (oil) and one or more polar fluids that are formed into the liquid droplets for droplet manipulations by the electrowetting force. In exemplary embodiments, therefore, an EWOD device includes a first substrate assembly and a second substrate assembly spaced apart to define a channel between the first and second substrate assemblies; an input port in fluid communication with the channel, the input port defining an input well for receiving a fluid for inputting into the channel; and a control port in fluid communication with the channel, the control port defining a control well for receiving a fluid and having a seal that seals the control port in a sealed state in which fluid is restricted from entering the control well from the channel. When the seal is removed or pierced, the control port is placed in an unsealed state thereby permitting fluid to enter the control well from the channel.
In the exemplary embodiment of
The EWOD device 100 further includes a control port 126, which in certain respects is configured comparably as the two input ports. Accordingly, the control port 126 includes a control wall 128 that is adhered to an outer surface of the top substrate assembly 102 by any suitable means. As above, rigid plastic materials provide a suitable material for the control wall 128. The control wall 128 defines a control well 130 that is in fluid communication with an exit port 132 that is configured as a hole that extends through the top substrate assembly 102. As above, the hole for the exit port may be formed by any suitable manufacturing process, such as drilling, stamping, cutting, or the like. Under certain conditions, which are described in more detail below, fluid can exit from the EWOD channel through the exit port 132 into the control well 130. The control port 126 further includes a seal 134 that is fixed to the control wall 128 to seal the control well 130 relative to the exterior of the EWOD device. The seal 134 may be removed or pierced to break such seal. The seal 134 thus may be made of any suitable material that can be removed or pierced, such as various tape materials, elastomeric materials that are adhered to the control wall, plastic cap materials, and the like. Tape materials are particularly suitable because they can be readily removed and replaced over multiple uses.
In this example, inner surfaces of the walls 114 and 120 coincide with boundaries of the respective channel ports 118 and 124. Similarly, an inner surface of the control wall 128 coincides with a boundary of the exit port 132. Such a configuration disrupts surface tension of a fluid located within the EWOD channel that is exposed to the channel ports and/or the exit port. As detailed below, disruption of the surface tension aids in providing a controlled input of fluids from the input ports through into the EWOD channel via the channel ports.
The EWOD device 100 further includes a vent port 136 that is configured as another hole that extends through the top substrate assembly 102. As above, the hole for the vent port may be formed by any suitable manufacturing process, such as drilling, stamping, cutting, or the like. The vent port 136 permits air to vent from the EWOD channel as fluid enters the EWOD channel, and thus prevents air from becoming trapped inside the EWOD channel.
Typically, a device operator uses a pipette to input polar fluid into the input ports 110 and/or 112 at a location adjacent to the respective channel port, e.g., channel port 118 and/or channel port 124. The polar fluid 140/142 typically is denser than the oil 138, so the pipetting action displaces the oil from the area adjacent to the channel ports 118 and 124 as shown in
The potential for such leakage may increase depending upon the precise makeup of the polar fluid. In particular, if the polar fluid contains a surfactant, a small fraction of the polar fluid may enter the EWOD channel, but such amounts do not undermine the device performance and is accommodated in subsequent steps. Accordingly, in most cases the amount of leakage into the EWOD channel will be essentially nil, and even when the polar fluid includes a surfactant, any leakage amounts to a small fraction such that the polar fluid at least substantially remains within the input wells 166 and 122 of the respective input ports 110 and 112, adjacent to the corresponding channel ports 118 and 124. The polar fluid thus remains as essentially a spherical reservoir at each of the channel ports.
Because of the presence of the seal 134, fluid from the polar fluid reservoirs 140 and 142 substantially does not enter the EWOD channel 108 during the fluid holding state at this point even if an electrowetting force is applied to the fluid in the input ports.
More particularly, when the seal 134 is removed or pierced, the system tries to balance the difference in the head of pressure of the input and control ports. Oil 138a is displaced into control port 126, and so there is now space inside the EWOD channel 108 to bring the polar fluid into the EWOD channel, as identified in the figures by reference numerals 140a and 142a. Input of the polar fluid into the EWOD channel is initiated by applying the appropriate electrowetting force. As referenced above, polar fluids generally resist entry into the EWOD channel due to the hydrophobic surfaces on the inner surface of the top substrate assembly 102. Accordingly, the system can be stable as shown in
Accordingly, when there are multiple fluid input ports being employed, the method of controlling fluid flow further may include the steps of: inputting polar fluid into the first input well to form a first fluid reservoir at a first channel port that fluidly connects the first input well to the channel, and inputting polar fluid into the second input well to form a second fluid reservoir at a second channel port that fluidly connects the second input well to the channel. When the seal is removed or pierced to place the control port in the unsealed state and a portion of the non-polar fluid reservoir within the channel displaces from the channel into the control well, a portion of the polar fluid from each of the first and second fluid reservoirs enters the channel respectively from the first and second input wells. The first fluid reservoir and the second fluid reservoir may contain either the same or different polar fluids. It will be appreciated that although two fluid input ports are employed in this example, any suitable number may be used as may be warranted for any given application.
In exemplary embodiments, when it is desired to input an additional volume of polar fluid that exceeds a maximum that can be handled within the EWOD channel at any one time, the processed polar fluid is extracted to ready the device for input of additional polar fluid into the EWOD channel. This may be accomplished by performing the following additional steps, and multiple cycles of polar fluid input/droplet manipulation/polar fluid extraction may be repeated.
The present invention has advantages over conventional filling mechanisms such as those described in the background section. U.S. Pat. No. 9,630,180 uses the hydrostatic head of pressure of the droplet actuator to allow fluid into the EWOD channel between the bottom and top substrates. Such device does not use a seal to create a large difference in hydrostatic pressure, and there is presumably a limit to the quantity of fluid that can be drawn on in this manner. WO 2017/047082 describes filling the device with oil, adding fluid to the input ports, and then then extracting a sufficient volume of oil to enable at least some of the fluid to enter the device. There is a limit to the quantity of fluid which can be processed by such a device, as it is determined by the amount of oil being extracted. This patent document does not recognize the potential for over filling the device and does not provide for any automated input of the polar fluid. Accordingly, the conventional configurations cannot remove oil and then load polar fluid for droplets into the EWOD channel, and then extract fluid from the device for subsequent input of additional polar fluid for droplets, in a controlled and automated manner with the use of a seal to initiate the fluid input.
In the system described herein, the system is stable in the fluid holding state until the user removes the seal from the control port to initiate the fluid input state. Sealing the control port creates a large difference in hydrostatic pressure between the input ports and the control port. When the seal is removed or pierced to place the control port in the unsealed state, the system tries to balance the hydrostatic pressure between the control port and the input ports. Oil then moves quickly into the control well of the control port, which allows the polar fluid to be inputted into, and to be removed from, the device efficiently and in a highly-controlled manner when the electrowetting force is applied.
Subsequent figures illustrate structural variations to the above. For ease of illustration, like structures are identified with like reference numerals as in the previous figures and generally are comparably configured. Additional explanation is provided as to the structural differences among the various embodiments. Relatedly, the various embodiments of the EWOD device generally will operate as described above, with certain modifications based on the structural variations as detailed below.
156 that is configured as a hole that extends through the top substrate assembly 102. The second control port 150 further includes a second seal 158 that is fixed to the second control wall 152 to seal the second control well 154 relative to the exterior of the EWOD device. The second seal 158 may be removed or pierced comparably as the first seal 134 to break such seal. The second seal likewise may be made of any suitable material that can be removed or pierced, such as various tape materials, elastomeric materials that are adhered to the control wall, plastic cap materials, and the like. Tape materials are particularly suitable because they can be readily removed and replaced over multiple uses.
The control ports 126 and 150 are operable independently of each other, and as such the seals 134 and 158 can be removed or pierced at different times as desired for any particular application. This allows more precise control over the rate at which fluids can be drawn into the EWOD channel. Operation proceeds as is described in connection with the first embodiment, but now a greater volume of oil can be displaced out of the system because two control ports may take up displaced oil through the independent exit ports 132 and 156. This in turn allows more polar fluid to be drawn into the EWOD channel using the electrowetting mechanism. Although this embodiment incorporates two independent control ports, any suitable number of independent control ports may be employed. Accordingly, when multiple independent control ports are used, the method of controlling fluid flow further may include the steps of: removing or piercing the first seal to place the first control port in an unsealed state, wherein a portion of the non-polar fluid within the channel is displaced from the channel into the first control well and a first draw of the polar fluid reservoir enters the channel from the input well; and removing or piercing the second seal to place the second control port in an unsealed state, wherein an additional portion of the non-polar fluid within the channel is displaced from the channel into the second control well and a second draw of the polar fluid reservoir enters the channel from the input well.
Referring to
As another feature of the example of
In this example, the first and second input ports 110 and 112 operate comparably as in previous embodiments. Because the walls 114 and 120 coincide with the boundaries of the channel ports 118 and 124, surface tension is disrupted as referenced above, and the first and second input ports may fill with oil. The first and second input ports also can hold a larger volume of polar fluid for generating liquid droplets using electrowetting once seal 134 on control port 126 is removed.
In contrast, the third input port 184 has a different configuration relative to the input ports 110 and 112. In particular, the wall 186 of the third input port is spaced apart from a boundary of the channel port and thus does not coincide with the boundary of the associated channel port 190 of the top substrate assembly. This means that in the region of the channel port 190, the surface tension is not disrupted at the channel port and thus the surface tension remains high. Accordingly, when the oil is filled into the EWOD channel (typically via one of the first two fluid input ports), oil does not enter the third input well 188 as the surface tension holds the oil back. The third input 184 can be used, for example, to pipette using the force of the pipette a small amount of polar fluid directly into the EWOD channel of the device. The third input port generally is not used to hold extra fluid for drawing droplets into the EWOD channel at a later time, as this port is best used only for small fluid volumes.
In contrast, in this embodiment the fluid input vent port 200 is configured comparably as the third input port 184 in
In exemplary embodiments, one of the control ports (e.g., the second port 232) first serves as a control port and then later as a fluid input port. The other control port (e.g., control port 126) first serves as a control port and then as a waste or exit port. In operation, the oil is added to the EWOD channel through the fluid input port 110. Excess oil fills this port. A polar fluid is then added to the fluid input port 110. The seal 240 from the second port 232 is then removed or pierced. The fluid and/or oil moves to try to balance the difference in the head of pressure between the two ports 110 and 232. The fluid from the first fluid input port 110 can therefore partially enter the EWOD channel at this point. Additional polar fluid then may be added to the second fluid input port 232 (which was initially a control port). When the user is ready to add the additional polar fluid, the seal 134 from the control port 126 is removed or pierced, and the fluid is drawn on from both input ports 110 and 232 using electrowetting comparably as in previous embodiments. An advantage of this embodiment is that it may make drawing challenging fluids into the device easier. For example, the difficult fluid, which is reluctant to enter the device, would be put in the first fluid input port 110. This fluid would then be subject to one seal being removed (which would act on this fluid only), and then the other seal being removed which would act on both fluids.
As another feature of this embodiment, port walls 114, 128, and 234 each may include a wall extension 242 that extends parallel to the first substrate assembly, which results in the wells having a smaller opening at the top. For the control port, the seal rests on the wall extension when the control port is in the sealed state. This wall extension 242 has a variety of advantages, including: (a) enabling the seals of the control ports to be secured more easily and with more support, (b) allowing the pipette to be guided into the correct position for pipetting fluid into the fluid input wells adjacent to the channel ports, and (c) minimizing oil evaporation from the wells particularly for applications in which the EWOD device may require heating.
As a further and exemplary embodiment, in all the previous embodiments, it will be appreciated that the top substrate could be formed as a single contiguous part, comprising one or more input ports and one or more control ports as has already been described. In this instance the one part top substrate could be comprised of plastic and manufactured, for example, by an injection molding process. An advantage of this embodiment is that a single part top substrate may simplifying the assembly of the device and/or reduce the manufacturing cost.
An aspect of the invention, therefore, is an electrowetting on dielectric (EWOD) device including an enhanced system for inputting fluid into the EWOD channel. In exemplary embodiments, the EWOD device includes a first substrate assembly and a second substrate assembly spaced apart to define a channel between the first and second substrate assemblies; an input port in fluid communication with the channel, the input port defining an input well for receiving a fluid for inputting into the channel; and a control port in fluid communication with the channel, the control port defining a control well for receiving a fluid and having a seal that seals the control port in a sealed state in which fluid is restricted from entering the control well from the channel. When the seal is removed or pierced, the control port is placed in an unsealed state thereby permitting fluid to enter the control well from the channel. The EWOD device may include one or more of the following features, either individually or in combination.
In an exemplary embodiment of the EWOD device, the input port includes an input wall that defines the input well and a channel port configured as a hole through the first substrate assembly to fluidly connect the input well to the channel, wherein an inner surface of the input wall coincides with a boundary of the channel port to disrupt surface tension of a fluid within the channel at the input port.
In an exemplary embodiment of the EWOD device, the input wall has a tapered inner surface that tapers toward the channel port.
In an exemplary embodiment of the EWOD device, the input wall has a wall extension that extends parallel to the first substrate assembly to provide a small opening into the input well.
In an exemplary embodiment of the EWOD device, the input port includes an input wall that defines the input well and a plurality of channel ports configured as holes through the first substrate assembly to fluidly connect the input well to the channel.
In an exemplary embodiment of the EWOD device, the EWOD device further includes a second input port in fluid communication with the channel, the second input port defining a second input well for receiving a fluid for inputting into the channel.
In an exemplary embodiment of the EWOD device, the second input port includes a second wall that defines the second input well and a second channel port configured as a hole through the first substrate assembly to fluidly connect the second input well to the channel, wherein an inner surface of the second wall coincides with a boundary of the second channel port to disrupt surface tension of a fluid within the channel at the second input port.
In an exemplary embodiment of the EWOD device, the second input port includes a second wall that defines the second input well and a second channel port configured as a hole through the first substrate assembly to fluidly connect the second input well to the channel, wherein an inner surface of the second wall is spaced apart from a boundary of the second channel port so as not to disrupt surface tension of a fluid within the channel at the second input port.
In an exemplary embodiment of the EWOD device, the second input port differs from the input port in at least one of well height, port diameter, channel port diameter, or wall material properties.
In an exemplary embodiment of the EWOD device, the control port includes a control wall that defines the control well and an exit port configured as a hole through the first substrate assembly to fluidly connect the control well to the channel, wherein an inner surface of the control wall coincides with a boundary of the exit port to disrupt surface tension of a fluid within the channel at the control port.
In an exemplary embodiment of the EWOD device, the control wall has a tapered inner surface that tapers toward the exit port.
In an exemplary embodiment of the EWOD device, the control wall has a wall extension that extends parallel to the first substrate assembly to provide a small opening into the control well, and the seal rests on the wall extension when the control port is in the sealed state.
In an exemplary embodiment of the EWOD device, the control port includes a control wall that defines the control well and a plurality of exit ports configured as holes through the first substrate assembly to fluidly connect the control well to the channel, wherein an inner surface of the control wall coincides with boundaries of the exit ports to disrupt surface tension of a fluid within the channel at the control port.
In an exemplary embodiment of the EWOD device, the control port includes at least one absorbent layer located within the control well for absorbing fluid that enters the control well.
In an exemplary embodiment of the EWOD device, the control port includes a plurality of absorbent layers located at different positions within the control well.
In an exemplary embodiment of the EWOD device, the EWOD device further includes a second control port in fluid communication with the channel, the second control port defining a second control well for receiving a fluid and having a second seal that seals the second control port in a sealed state in which fluid is restricted from entering the second control port. When the second seal is removed or pierced, the second control port is in an unsealed state thereby permitting fluid to enter the second control well from the channel.
In an exemplary embodiment of the EWOD device, the second control port includes a second control wall that defines the second control well and a second exit port configured as a hole through the first substrate assembly to fluidly connect the control well to the channel, wherein an inner surface of the second control wall coincides with a boundary of the second exit port to disrupt surface tension of a fluid within the channel at the second control port.
In an exemplary embodiment of the EWOD device, the EWOD device further includes a second control port in fluid communication with the channel, the second control port defining a second control well for receiving a fluid and having a second seal that seals the second control port in a sealed state in which fluid is restricted from entering the second control port. When the second seal is removed or pierced, the second control port is in an unsealed state thereby permitting the second control port to act as an input port for inputting fluid from the second control well into the channel.
In an exemplary embodiment of the EWOD device, the second control port includes a second control wall that defines the second control well and another channel port configured as a hole through the first substrate assembly to fluidly connect the second control well to the channel, wherein an inner surface of the second control wall coincides with a boundary of the another channel port to disrupt surface tension of a fluid within the channel at the second control port.
In an exemplary embodiment of the EWOD device, the EWOD device further includes a vent port in fluid communication with the EWOD channel, wherein the vent port vents air from the EWOD channel as fluid enters the EWOD channel from the input port.
In an exemplary embodiment of the EWOD device, the vent port is configured as another input port in fluid communication with the channel, the vent port defining a vent port input well for receiving a fluid for inputting into the channel.
In an exemplary embodiment of the EWOD device, the vent port includes a vent port wall that defines the vent port input well and a vent port channel port configured as a hole through the first substrate assembly to fluidly connect the vent port input well to the channel, wherein an inner surface of the vent port wall coincides with a boundary of the vent port channel port to disrupt surface tension of a fluid within the channel at the vent port.
In an exemplary embodiment of the EWOD device, the vent port includes a vent port wall that defines the vent port input well and a vent port channel port configured as a hole through the first substrate assembly to fluidly connect the vent port input well to the channel, wherein an inner surface of the vent port wall is spaced apart from a boundary of the vent port channel port so as not to disrupt surface tension of a fluid within the channel at the vent port.
In an exemplary embodiment of the EWOD device, the EWOD device further includes a first cover that is fixed on top of the first substrate assembly, and the input port is incorporated into the first cover.
In an exemplary embodiment of the EWOD device, the EWOD device further includes a second cover that is fixed on top of the first substrate assembly, and the control port is incorporated into the second cover.
In an exemplary embodiment of the EWOD device, the EWOD device further includes a vent port in fluid communication with the EWOD channel, wherein the vent port vents air from the EWOD channel as fluid enters the EWOD channel from the input port, and the vent port is incorporated into the second cover.
The described EWOD device according to any of the above embodiments may be employed to perform an enhanced method of controlling fluid flow through an electrowetting on dielectric (EWOD) device. In exemplary embodiments, the method of controlling fluid flow may include the steps of providing said EWOD device; inputting a non-polar fluid into the channel via the input port, wherein excess non-polar fluid after filling the EWOD channel accumulates within the input well and is restricted from entering the control well by the seal; inputting a polar fluid into the input well to form a fluid reservoir at a channel port that fluidly connects the input well to the channel; and removing or piercing the seal to place the control port in an unsealed state, wherein a portion of the non-polar fluid within the channel is displaced from the channel into the control well, and a portion of the polar fluid of the fluid reservoir enters the channel from the input well. The method of controlling fluid may include one or more of the following steps, either individually or in combination.
In an exemplary embodiment of the method of controlling fluid flow, the method further may include, after the seal is removed or pierced, applying an electrowetting force to dispense one or more liquid droplets from the fluid reservoir for manipulation within the channel.
In an exemplary embodiment of the method of controlling fluid flow, the method further may include: applying the electrowetting force to move the dispensed droplets to an exit port in fluid communication with the EWOD channel; forming an exit reservoir of the moved dispensed droplets adjacent to the exit port; and deactivating the electrowetting force in a region of the exit port, wherein the exit reservoir exits the channel via the exit port under a hydrostatic force.
In an exemplary embodiment of the method of controlling fluid flow, the exit port is a port that fluidly connects the channel to the control well of the control port.
In an exemplary embodiment of the method of controlling fluid flow, the method further may include performing a plurality of fluid flow cycles after the seal is removed or pierced, wherein each fluid flow cycle comprises the steps of: applying an electrowetting force to dispense one or more liquid droplets from the fluid reservoir for manipulation within the channel; applying the electrowetting force to move the dispensed droplets to the exit port; forming an exit reservoir of the moved dispensed droplets adjacent to the exit port; and deactivating the electrowetting force in the region of the exit port, wherein the exit reservoir exits the channel via the exit port under a hydrostatic force.
In an exemplary embodiment of the method of controlling fluid flow, the EWOD device includes: a first control port in fluid communication with the channel, the first control port defining a first control well for receiving a fluid and having a first seal that seals the first control port in a sealed state; and a second control port in fluid communication with the channel, the second control port defining a second control well for receiving a fluid and having a second seal that seals the second control port in a sealed state. The method of controlling fluid flow further comprising the steps of: removing or piercing the first seal to place the first control port in an unsealed state, wherein a portion of the non-polar fluid within the channel is displaced from the channel into the first control well and a first draw of the polar fluid reservoir enters the channel from the input well; and removing or piercing the second seal to place the second control port in an unsealed state, wherein an additional portion of the non-polar fluid within the channel is displaced from the channel into the second control well and a second draw of the polar fluid reservoir enters the channel from the input well.
In an exemplary embodiment of the method of controlling fluid flow, the EWOD device includes: a first input port in fluid communication with the channel, the first input port defining a first input well for receiving a fluid for inputting into the channel; and a second input port in fluid communication with the channel, the second input port defining a second input well for receiving a fluid for inputting into the channel. The method of controlling fluid flow further comprising the steps of: inputting polar fluid into the first input well to form a first fluid reservoir at a first channel port that fluidly connects the first input well to the channel; and inputting polar fluid into the second input well to form a second fluid reservoir at a second channel port that fluidly connects the second input well to the channel. When the seal is removed or pierced to place the control port in the unsealed state and a portion of the non-polar fluid reservoir within the channel displaces from the channel into the control well, a portion of the polar fluid from each of the first and second fluid reservoirs enters the channel respectively from the first and second input wells.
In an exemplary embodiment of the method of controlling fluid flow, the first fluid reservoir and the second fluid reservoir contain different polar fluids
Although the invention has been shown and described with respect to a certain embodiment or embodiments, equivalent alterations and modifications may occur to others skilled in the art upon the reading and understanding of this specification and the annexed drawings. In particular regard to the various functions performed by the above described elements (components, assemblies, devices, compositions, etc.), the terms (including a reference to a “means”) used to describe such elements are intended to correspond, unless otherwise indicated, to any element which performs the specified function of the described element (i.e., that is functionally equivalent), even though not structurally equivalent to the disclosed structure which performs the function in the herein exemplary embodiment or embodiments of the invention. In addition, while a particular feature of the invention may have been described above with respect to only one or more of several embodiments, such feature may be combined with one or more other features of the other embodiments, as may be desired and advantageous for any given or particular application.
The described embodiments could be used to provide an enhanced AM-EWOD device. The AM-EWOD device could form a part of a lab-on-a-chip system. Such devices could be used in manipulating, reacting and sensing chemical, biochemical or physiological materials. Applications include healthcare diagnostic testing, material testing, chemical or biochemical material synthesis, proteomics, tools for research in life sciences and forensic science.
| Number | Name | Date | Kind |
|---|---|---|---|
| 5975153 | Hill | Nov 1999 | A |
| 20140014517 | Srinivasan | Jan 2014 | A1 |
| 20150174578 | Srinivasan | Jun 2015 | A1 |
| 20160074863 | Sturmer | Mar 2016 | A1 |
| 20160195492 | Bauer | Jul 2016 | A1 |
| Number | Date | Country | |
|---|---|---|---|
| 20180369814 A1 | Dec 2018 | US |
