Claims
- 1. A method for culturing hematopoietic cells, comprising: culturing a population of human hematopoietic cells comprising stem cells or committed progenitor cells in a stromal conditioned medium in the absence of stromal cells, wherein the medium comprises an effective added amount of one or more cytokines selected from the group consisting of MIP-1.alpha., IL-3, SCF, BB10010 and PF-4, so that the ability of the stem cells to self-replicate and differentiate is maintained or the ability of the committed progenitors to expand and differentiate is maintained.
- 2. The method of claim 1 wherein the hematopoietic cells consist essentially of committed progenitor cells.
- 3. The method of claim 1 wherein the hematopoietic cells comprise stem cells.
- 4. The method of claim 1 wherein the hematopoietic cells are derived from bone marrow cells.
- 5. The method of claim 1 wherein the hematopoietic cells are chronic myelogenous leukemia bone marrow cells.
- 6. The method of claim 1 wherein the hematopoietic cells are derived from peripheral blood or from newborn cord blood.
- 7. The method of claim 1 wherein the hematopoietic cell population is allogeneic.
- 8. The method of claim 1 wherein the hematopoietic cell population is autologous.
- 9. The method of claim 2 wherein the hematopoietic cells consist essentially of natural killer cells.
- 10. The method of claim 3 wherein the stem cells are CD34.sup.+.
- 11. The method of claim 10 wherein the stem cells are Lin.sup.- CD34.sup.+DR.sup.-.
Parent Case Info
This application is a 371 application of PCT/US96/18222, filed Nov. 13, 1996, and claims priority under 35 U.S.C. .sctn. 119(e), to U.S. provisional application Serial No. 60/006,737, filed Nov. 14, 1995 (the '737 Application). The '737 application is incorporated herein by reference.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
102e Date |
371c Date |
PCT/US96/18222 |
11/13/1996 |
|
|
7/14/1997 |
7/14/1997 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO97/18298 |
5/22/1997 |
|
|
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|
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|
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WOX |
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Oct 1993 |
WOX |
9513088 |
May 1995 |
WOX |
9602662 |
Feb 1996 |
WOX |
Non-Patent Literature Citations (2)
Entry |
Tamura et al., British Journal of Haematology, vol. 81, 1992, pp. 353-361. |
Guido J. Tricot, Leukemia Res., vol. 16, No. 1, pp. 109-115, 1992. |