Ex vivo protease activity detection for disease detection/diagnostic, staging, monitoring and treatment

Information

  • Patent Grant
  • 11851697
  • Patent Number
    11,851,697
  • Date Filed
    Tuesday, January 11, 2022
    2 years ago
  • Date Issued
    Tuesday, December 26, 2023
    12 months ago
Abstract
The present application provides compositions and methods for determining a disease or condition in a subject. The method comprises contacting a body fluid with a molecule comprising a reporter thereof and the reported is cleaved by an agent in the body fluid. Diseases and conditions that can be determined by the method are also described.
Description
SEQUENCE LISTING

The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jan. 11, 2022, is named 61226_702_501_SL.txt and is 393,420 bytes in size.


BRIEF SUMMARY

Provided herein is a method comprising contacting a plasma sample from a subject with a molecule ex vivo and detecting a rate of formation or an amount of said released reporter. Further provided herein is a method. Further provided herein is a method wherein said molecule comprises a cleavable linker and a reporter and wherein said cleavable linker is cleaved by an agent from said plasma, releasing said reporter from said molecule.


Further provided herein is a method further comprising introducing an anticoagulant to said plasma sample. Further provided herein is a method wherein said anticoagulant is an EDTA, a citrate, a heparin, an oxalate, any salt, solvate, enantiomer, tautomer and geometric isomer thereof, or any mixtures thereof.


Provided herein is a method comprising contacting a body fluid sample from a subject having a disease or condition with a molecule ex vivo. Further provided herein is a method wherein said molecule comprises a cleavable linker and a reporter and wherein said cleavable linker is cleaved by an agent from said body fluid, releasing said reporter from said molecule. Further provided herein is a method wherein said rate of formation or said amount of said released reporter is significantly different from a healthy subject.


Provided herein is a method comprising contacting a body fluid sample from a subject with a first molecule ex vivo wherein said first molecule comprises a first cleavable linker and a first reporter and wherein said first cleavable linker is cleaved by a first agent from said body fluid, releasing said first reporter from said first molecule. Further provided herein is a method detecting a rate of formation or an amount of said first released reporter. Further provided herein is a method contacting said body fluid sample from said subject with a second molecule ex vivo wherein said second molecule comprises a second cleavable linker and a second reporter, and wherein said second cleavable linker is cleaved by a second agent from said body fluid, releasing said second reporter from said second molecule. Further provided herein is a method detecting a rate of formation or an amount of said second released reporter and determining a disease or condition of said subject based on said detection of said first released reporter and said detection of said second released reporter.


Further provided herein is a method wherein said determination comprises a supervised Machine Learning classification algorithm, Logistic Regression, Naive Bayes, Support Vector Machine, Random Forest, Gradient Boosting, Neural Networks, a continuous regression approach, Ridge Regression, Kernel Ridge Regression, Support Vector Regression or any combination thereof.


Provided herein is a method comprising contacting a body fluid sample from a subject with a molecule ex vivo, wherein said molecule comprises a cleavable linker and a reporter and wherein said cleavable linker is cleaved by an agent from said body fluid, releasing said reporter from said molecule. Further provided herein is a method comprising detecting a rate of formation or an amount of said released reporter and determining a disease or condition of said subject based on said detection, wherein said disease or condition is a certain fibrosis stage or a certain nonalcoholic fatty liver disease activity score (NAS) of Non-alcoholic steatohepatitis (NASH).


Provided herein is a method comprising contacting a body fluid sample from a subject with a molecule ex vivo wherein said molecule comprises a cleavable linker and a reporter and wherein said cleavable linker is cleaved by an agent from said body fluid, releasing said reporter from said molecule. Further provided herein is a method detecting a rate of formation or an amount of said released reporter and determining a disease or condition of said subject based on said detection, wherein said disease or condition is selected from the group consisting of a liver disease a cancer, an organ transplant rejection, an infectious disease, an allergic disease, an autoimmunity, an Alzheimer's and a chronic inflammation; wherein said cancer is not pancreatic ductal adenocarcinoma or non-small cell lung cancer.


Further provided herein is a method wherein said liver disease comprises a Non-alcoholic steatohepatitis (NASH), a non-alcoholic fatty liver disease (NAFLD), a toxin mediated liver injury, a viral hepatitis, a fulminant hepatitis, an alcoholic hepatitis, an autoimmune hepatitis, a cirrhosis of the liver, a hepatocellular carcinoma (HCC), a primary biliary cholangitis (PBC), a cholangiocarcinoma, a primary sclerosing cholangitis, an acute or chronic rejection of a transplanted liver, an inherited liver disease or a combination thereof.


Further provided herein is a method wherein said body fluid sample is selected from the group consisting of blood, plasma, bone marrow fluid, lymphatic fluid, bile, amniotic fluid, mucosal fluid, saliva, urine, cerebrospinal fluid, spinal fluid, synovial fluid, semen, ductal aspirate, feces, stool, vaginal effluent, lachrymal fluid, tissue lysate and patient-derived cell line supernatant.


Further provided herein is a method wherein said body fluid sample comprises a rinse fluid, a conditioning media or buffer, a swab viral transport media, a saline, a culture media, or a cell culture supernatant.


Further provided herein is a method wherein said rinse fluid is selected from the group consisting of a mouthwash rinse, a bronchioalveolar rinse, a lavage fluid, a hair wash rinse, a nasal spray effluent, a swab of any bodily surface, orifice, organ structure or solid tumor biopsies applied to saline or any media or any derivatives thereof.


Further provided herein is a method wherein said agent is selected from the group consisting of a oxidoreductase, a transferase, a hydrolase, a lyase, a isomerase, a ligase, a protease (peptidase), a hydrolase, an esterase, a β-glycosidase, a phospholipase and a phosphodiesterase, peroxidase, lipase, amylase a nucleophilic reagent, a reducing reagent, a electrophilic/acidic reagent, an organometallic/metal catalyst, an oxidizing reagent, a hydroxyl ion, a thiols nucleophile, a nitrogen nucleophile, a sodium dithionite and a sodium periodate.


Further provided herein is a method wherein said agent is a protease. Further provided herein is a method wherein said protease is an endopeptidase or an exopeptidase. Further provided herein is a method wherein said protease is selected from the group consisting of an A20 (TNFa-induced protein 3), an abhydrolase domain containing 4, an abhydrolase domain containing 12, an abhydrolase domain containing 12B, an abhydrolase domain containing 13, an acrosin, an acylaminoacyl-peptidase, a disintegrin and metalloproteinase (ADAM), an ADAM1a, an ADAM2 (Fertilin-b), an ADAM3B, an ADAM4, an ADAM4B, an ADAM5, an ADAM6, an ADAM7, an ADAM8, an ADAM9, an ADAM10, an ADAM11, an ADAM12 metalloprotease, an ADAM15, an ADAM17, an ADAM18, an ADAM19, an ADAM20, an ADAM21, an ADAM22, an ADAM23, an ADAM28, an ADAM29, an ADAM30, an ADAM32, an ADAM33, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), an ADAMTS1, an ADAMTS2, an ADAMTS3, an ADAMTS4, an ADAMTS5/11, an ADAMTS6, an ADAMTS7, an ADAMTS8, an ADAMTS9, an ADAMTS10, an ADAMTS12, an ADAMTS13, an ADAMTS14, an ADAMTS15, an ADAMTS16, an ADAMTS17, an ADAMTS18, an ADAMTS19, an ADAMTS20, an adipocyte-enh. binding protein 1, an Afg3-like protein 1, an Afg3-like protein 2, an airway-trypsin-like protease, an aminoacylase, an aminopeptidase A, an aminopeptidase B, an aminopeptidase B-like 1, an aminopeptidase MAMS/L-RAP, an aminopeptidase N, an aminopeptidase O, an aminopeptidase P homologue, an aminopeptidase P1, an aminopeptidase PILS, an aminopeptidase Q, an aminopeptidase-like 1, an AMSH/STAMBP, an AMSH-LP/STAMBPL1, an angiotensin-converting enzyme 1 (ACE1), an angiotensin-converting enzyme 2 (ACE2), an angiotensin-converting enzyme 3 (ACE3), an anionic trypsin (II), an apolipoprotein (a), an archaemetzincin-1, an archaemetzincin-2, an aspartoacylase, an aspartoacylase-3, an aspartyl aminopeptidase, an ataxin-3, an ataxin-3 like, an ATP/GTP binding protein 1, an ATP/GTP binding protein-like 2, an ATP/GTP binding protein-like 3, an ATP/GTP binding protein-like 4, an ATP/GTP binding protein-like 5, an ATP23 peptidase, an autophagin-1, an autophagin-2, an autophagin-3, an autophagin-4, an azurocidin, a beta lactamase, a beta-secretase 1, a beta-secretase 2, a bleomycin hydrolase, a brain serine proteinase 2, a BRCC36 (BRCA2-containing complex, sub 3), a calpain, a calpain 1, a calpain 2, a calpain 3, a calpain 4, a calpain 5, a calpain 6, a calpain 7, a calpain 7-like, a calpain 8, a calpain 9, a calpain 10, a calpain 11, a calpain 12, a calpain 13, a calpain 14, a calpain 15 (Solh protein), a cysteine protease, a carboxypeptidase A1, a carboxypeptidase A2, a carboxypeptidase A3, a carboxypeptidase A4, a carboxypeptidase A5, a carboxypeptidase A6, a carboxypeptidase B, a carboxypeptidase D, a carboxypeptidase E, a carboxypeptidase M, a carboxypeptidase N, a carboxypeptidase O, a carboxypeptidase U, a carboxypeptidase X1, a carboxypeptidase X2, a carboxypeptidase Z, a carnosine dipeptidase 1, a carnosine dipeptidase 2, a caspase recruitment domain family, member 8, a caspase, a caspase-1, a caspase-2, a caspase-3, a caspase-4/11, a caspase-5, a caspase-6, a caspase-7, a caspase-8, a caspase-9, a caspase-10, a caspase-12, a caspase-14, a caspase-14-like, a casper/FLIP, a cathepsin, a cathepsin A (CTSA), a cathepsin B (CTSB), a cathepsin C (CTSC), a cathepsin D (CTSD), a cathepsin E (CTSE), a cathepsin F, a cathepsin G, a cathepsin H (CTSH), a cathepsin K (CTSK), a cathepsin L (CTSL), a cathepsin L2, a cathepsin O, a cathepsin S (CTSS), a cathepsin V (CTSV), a cathepsin W, a cathepsin Z (CTSZ), a cationic trypsin, a cezanne/OTU domain containing 7B, a cezanne-2, a CGI-58, a chymase, a chymopasin, a chymosin, a chymotrypsin B, a chymotrypsin C, a coagulation factor IXa, a coagulation factor VIIa, a coagulation factor Xa, a coagulation factor XIa, a coagulation factor XIIa, a collagenase 1, a collagenase 2, a collagenase 3, a complement protease C1r serine protease, a complement protease C1s serine protease, a complement C1r-homolog, a complement component 2, a complement component C1ra, a complement component C1sa, a complement factor B, a complement factor D, a complement factor D-like, a complement factor I, a COPS6, a corin, a CSN5 (JAB1), a cylindromatosis protein, a cytosol alanyl aminopep.-like 1, a cytosol alanyl aminopeptidase, a DDI-related protease, a DECYSIN, a Der1-like domain family, member 1, a Der1-like domain family, member 2, a Der1-like domain family, member 3, a DESC1 protease, a desert hedgehog protein, a desumoylating isopeptidase 1, a desumoylating isopeptidase 2, a dihydroorotase, a dihydropyrimidinase, a dihydropyrimidinase-related protein 1, a dihydropyrimidinase-related protein 2, a dihydropyrimidinase-related protein 3, a dihydropyrimidinase-related protein 4, a dihydropyrimidinase-related protein 5, a DINE peptidase, a dipeptidyl peptidase (DPP), a dipeptidyl peptidase (DPP1), a dipeptidyl-peptidase 4 (DPP4), a dipeptidyl-peptidase 6 (DPP6), a dipeptidyl-peptidase 8 (DPP8), a dipeptidyl-peptidase 9 (DPP9), a dipeptidyl-peptidase II, a dipeptidyl-peptidase III, a dipeptidyl-peptidase 10 (DPP10), a DJ-1, a DNA-damage inducible protein, a DNA-damage inducible protein 2, a DUB-1, a DUB-2, a DUB2a, a DUB2a-like, a DUB2a-like2, a DUB6, or a combination thereof. Further provided herein is a method wherein said protease is selected from the group consisting of a T cell protease, a complement protease, a fibrosis protease, and an inflammation-related protease.


Further provided herein is a method wherein said cleavable linker is a peptide, a carbohydrate, a nucleic acid, a lipid, an ester, a glycoside, a phospholipid, a phosphodiester, a nucleophile/base sensitive linker, a reduction sensitive linker, an electrophile/acid sensitive linker, a metal cleavable linker, an oxidation sensitive linker or a combination thereof. Further provided herein is a method wherein said cleavable linker is a peptide. Further provided herein is a method wherein said peptide comprises an amino acid sequence selected from the group consisting of SEQ ID Nos: 1-677.


Further provided herein is a method wherein said cleavable linker is directly connected to said reporter through a covalent bond. Further provided herein is a method wherein said reporter comprises a fluorescent label, a mass tag, a chromophore, an electrochemically active molecule, a bio-Layer interferometry or surface plasmon resonance detectable molecule, a precipitating substance, a mass spectrometry and liquid chromatography substrate, a magnetically active molecule, a gel forming and/or viscosity changing molecule, an immunoassay detectable molecule, a cell-based amplification detectable or a nucleic acid barcode, or any combinations thereof. Further provided herein is a method wherein said reporter comprises a fluorescent label. Further provided herein is a method wherein said fluorescent label is selected from a group consisting of a 5-carboxyfluorescein (5-FAM), a 7-amino-4-carbamoylmethylcoumarin (ACC), a 7-Amino-4-methylcoumarin (AMC), a 2-Aminobenzoyl (Abz), a Cy7, a Cy5, a Cy3 and a (5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid) (EDANS).


Further provided herein is a method wherein said molecule further comprises a fluorescent quencher. Further provided herein is a method wherein said fluorescent quencher is selected from the group consisting of BHQ0, BHQ1, BHQ2, BHQ3, BBQ650, ATTO 540Q, ATTO 580Q, ATTO 612Q, CPQ2, QSY-21, QSY-35, QSY-7, QSY-9, DABCYL (4-([4′-dimethylamino)phenyl]azo)benzoyl), Dnp (2,4-dinitrophenyl) and Eclipse. Further provided herein is a method wherein said fluorescent quencher is directly connected to said cleavable linker through a covalent bond.


Further provided herein is a method wherein said molecule further comprises a carrier. Further provided herein is a method wherein said carrier comprises a native, labeled or synthetic protein, a synthetic chemical polymer of precisely known chemical composition or with a distribution around a mean molecular weight, an oligonucleotide, a phosphorodiamidate morpholino oligomer (PMO), a foldamer, a lipid, a lipid micelle, a nanoparticle, a solid support made of polystyrene, polypropylene or any other type of plastic, or any combination thereof.


Further provided herein is a method wherein said subject is a mammal. Further provided herein is a method wherein said mammal is a human.


Further provided herein is a method wherein said reporter is linked to said cleavable linker through a self-immolative spacer. Further provided herein is a method wherein said self-immolative spacer is selected from the group consisting of a disulfide, a hetheroaminebifuncional disulfide, a thiol-based pirydazinediones, a p-aminebenzyloxycarbonyl, a dipeptide, a Gly-Pro (SEQ ID NO: 530), a L-Phe-Sar, a trans-cyclooctene tetrazine, a ortho Hydroxy-protected Aryl sulfate, a phosphoramidate-based spacer, a hydroxybenzyl, a trimethyl carbamate and a quinone methide-based spacer.


Further provided herein is a method wherein said detection comprises fluorescent detection, spectroscopic detection, mass spectrometry, immunological detection or imaging detection. Further provided herein is a method wherein said detection comprises fluorescent detection. Further provided herein is a method wherein said fluorescent detection is fluorescence resonance energy transfer (FRET).


Further provided herein is a method wherein said cleaved reporter comprises a precipitating fluorophore. Further provided herein is a method wherein said precipitating fluorophore comprises HPQ, Cl-HPQ, HTPQ, HBPQ, or HQPQ.


Provided herein is a method comprising measuring activity of two or more agents in a body fluid sample from a subject and determining a disease or condition of said subject based on said activity wherein said disease or condition is selected from the group consisting of a liver disease, an organ transplant rejection, an infectious disease, an allergic disease, an autoimmunity, an Alzheimer's and a chronic inflammation.


Further provided herein is a method wherein said liver disease comprises a Non-alcoholic steatohepatitis (NASH), a non-alcoholic fatty liver disease (NAFLD), a toxin mediated liver injury, a viral hepatitis, a fulminant hepatitis, an alcoholic hepatitis, an autoimmune hepatitis, a cirrhosis of the liver, a hepatocellular carcinoma (HCC), a primary biliary cholangitis (PBC), a cholangiocarcinoma, a primary sclerosing cholangitis, an acute or chronic rejection of a transplanted liver, an inherited liver disease or a combination thereof.


Provided herein is a method comprising measuring activity of two or more agents in a body fluid sample from a subject and determining a disease or condition of said subject based on said activity wherein said disease or condition is a certain fibrosis stage or a certain nonalcoholic fatty liver disease activity score (NAS) of Non-alcoholic steatohepatitis (NASH).


Further provided herein is a method which further comprises contacting said body fluid sample from said subject with a molecule ex vivo, wherein said molecule comprises a cleavable linker and a reporter and wherein said cleavable linker is cleaved by said protease from said plasma, releasing said reporter from said molecule, and detecting a rate of formation or an amount of said released reporter.


Further provided herein is a method wherein said agent is selected from the group consisting of a oxidoreductase, a transferase, a hydrolase, a lyase, a isomerase, a ligase, a protease (peptidase), a hydrolase, an esterase, a β-glycosidase, a phospholipase and a phosphodiesterase, peroxidase, lipase, amylase a nucleophilic reagent, a reducing reagent, a electrophilic/acidic reagent, an organometallic/metal catalyst, an oxidizing reagent, a hydroxyl ion, a thiols nucleophile, a nitrogen nucleophile, a sodium dithionite and a sodium periodate. Further provided herein is a method wherein said agent is a protease. Further provided herein is a method wherein said protease is an endopeptidase or an exopeptidase. Further provided herein is a method wherein said protease is selected from the group consisting of an A20 (TNFa-induced protein 3), an abhydrolase domain containing 4, an abhydrolase domain containing 12, an abhydrolase domain containing 12B, an abhydrolase domain containing 13, an acrosin, an acylaminoacyl-peptidase, a disintegrin and metalloproteinase (ADAM), an ADAM1a, an ADAM2 (Fertilin-b), an ADAM3B, an ADAM4, an ADAM4B, an ADAM5, an ADAM6, an ADAM7, an ADAM8, an ADAM9, an ADAM10, an ADAM11, an ADAM12 metalloprotease, an ADAM15, an ADAM17, an ADAM18, an ADAM19, an ADAM20, an ADAM21, an ADAM22, an ADAM23, an ADAM28, an ADAM29, an ADAM30, an ADAM32, an ADAM33, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), an ADAMTS1, an ADAMTS2, an ADAMTS3, an ADAMTS4, an ADAMTS5/11, an ADAMTS6, an ADAMTS7, an ADAMTS8, an ADAMTS9, an ADAMTS10, an ADAMTS12, an ADAMTS13, an ADAMTS14, an ADAMTS15, an ADAMTS16, an ADAMTS17, an ADAMTS18, an ADAMTS19, an ADAMTS20, an adipocyte-enh. binding protein 1, an Afg3-like protein 1, an Afg3-like protein 2, an airway-trypsin-like protease, an aminoacylase, an aminopeptidase A, an aminopeptidase B, an aminopeptidase B-like 1, an aminopeptidase MAMS/L-RAP, an aminopeptidase N, an aminopeptidase O, an aminopeptidase P homologue, an aminopeptidase P1, an aminopeptidase PILS, an aminopeptidase Q, an aminopeptidase-like 1, an AMSH/STAMBP, an AMSH-LP/STAMBPL1, an angiotensin-converting enzyme 1 (ACE1), an angiotensin-converting enzyme 2 (ACE2), an angiotensin-converting enzyme 3 (ACE3), an anionic trypsin (II), an apolipoprotein (a), an archaemetzincin-1, an archaemetzincin-2, an aspartoacylase, an aspartoacylase-3, an aspartyl aminopeptidase, an ataxin-3, an ataxin-3 like, an ATP/GTP binding protein 1, an ATP/GTP binding protein-like 2, an ATP/GTP binding protein-like 3, an ATP/GTP binding protein-like 4, an ATP/GTP binding protein-like 5, an ATP23 peptidase, an autophagin-1, an autophagin-2, an autophagin-3, an autophagin-4, an azurocidin, a beta lactamase, a beta-secretase 1, a beta-secretase 2, a bleomycin hydrolase, a brain serine proteinase 2, a BRCC36 (BRCA2-containing complex, sub 3), a calpain, a calpain 1, a calpain 2, a calpain 3, a calpain 4, a calpain 5, a calpain 6, a calpain 7, a calpain 7-like, a calpain 8, a calpain 9, a calpain 10, a calpain 11, a calpain 12, a calpain 13, a calpain 14, a calpain 15 (Solh protein), a cysteine protease, a carboxypeptidase A1, a carboxypeptidase A2, a carboxypeptidase A3, a carboxypeptidase A4, a carboxypeptidase A5, a carboxypeptidase A6, a carboxypeptidase B, a carboxypeptidase D, a carboxypeptidase E, a carboxypeptidase M, a carboxypeptidase N, a carboxypeptidase O, a carboxypeptidase U, a carboxypeptidase X1, a carboxypeptidase X2, a carboxypeptidase Z, a carnosine dipeptidase 1, a carnosine dipeptidase 2, a caspase recruitment domain family, member 8, a caspase, a caspase-1, a caspase-2, a caspase-3, a caspase-4/11, a caspase-5, a caspase-6, a caspase-7, a caspase-8, a caspase-9, a caspase-10, a caspase-12, a caspase-14, a caspase-14-like, a casper/FLIP, a cathepsin, a cathepsin A (CTSA), a cathepsin B (CTSB), a cathepsin C (CTSC), a cathepsin D (CTSD), a cathepsin E (CTSE), a cathepsin F, a cathepsin G, a cathepsin H (CTSH), a cathepsin K (CTSK), a cathepsin L (CTSL), a cathepsin L2, a cathepsin O, a cathepsin S (CTSS), a cathepsin V (CTSV), a cathepsin W, a cathepsin Z (CTSZ), a cationic trypsin, a cezanne/OTU domain containing 7B, a cezanne-2, a CGI-58, a chymase, a chymopasin, a chymosin, a chymotrypsin B, a chymotrypsin C, a coagulation factor IXa, a coagulation factor VIIa, a coagulation factor Xa, a coagulation factor XIa, a coagulation factor XIIa, a collagenase 1, a collagenase 2, a collagenase 3, a complement protease C1r serine protease, a complement protease Cis serine protease, a complement C1r-homolog, a complement component 2, a complement component C1ra, a complement component C1sa, a complement factor B, a complement factor D, a complement factor D-like, a complement factor I, a COPS6, a corin, a CSN5 (JAB1), a cylindromatosis protein, a cytosol alanyl aminopep.-like 1, a cytosol alanyl aminopeptidase, a DDI-related protease, a DECYSIN, a Der1-like domain family, member 1, a Der1-like domain family, member 2, a Der1-like domain family, member 3, a DESC1 protease, a desert hedgehog protein, a desumoylating isopeptidase 1, a desumoylating isopeptidase 2, a dihydroorotase, a dihydropyrimidinase, a dihydropyrimidinase-related protein 1, a dihydropyrimidinase-related protein 2, a dihydropyrimidinase-related protein 3, a dihydropyrimidinase-related protein 4, a dihydropyrimidinase-related protein 5, a DINE peptidase, a dipeptidyl peptidase (DPP), a dipeptidyl peptidase (DPP1), a dipeptidyl-peptidase 4 (DPP4), a dipeptidyl-peptidase 6 (DPP6), a dipeptidyl-peptidase 8 (DPP8), a dipeptidyl-peptidase 9 (DPP9), a dipeptidyl-peptidase II, a dipeptidyl-peptidase III, a dipeptidyl-peptidase 10 (DPP10), a DJ-1, a DNA-damage inducible protein, a DNA-damage inducible protein 2, a DUB-1, a DUB-2, a DUB2a, a DUB2a-like, a DUB2a-like2, a DUB6, or a combination thereof.


Further provided herein is a method wherein said protease is selected from the group consisting of a T cell protease, a complement protease, a fibrosis protease, and an inflammation-related protease. Further provided herein is a method wherein said cleavable linker is a peptide, a carbohydrate, a nucleic acid, a lipid, an ester, a glycoside, a phospholipid, a phosphodiester, a nucleophile/base sensitive linker, a reduction sensitive linker, an electrophile/acid sensitive linker, a metal cleavable linker, an oxidation sensitive linker or a combination thereof. Further provided herein is a method wherein said cleavable linker is a peptide. Further provided herein is a method wherein said peptide comprises an amino acid sequence selected from the group consisting of SEQ ID Nos: 1-677. Further provided herein is a method wherein said cleavable linker is directly connected to said reporter through a covalent bond.


Further provided herein is a method wherein said reporter comprises a fluorescent label, a mass tag, a chromophore, an electrochemically active molecule, a bio-Layer interferometry or surface plasmon resonance detectable molecule, a precipitating substance, a mass spectrometry and liquid chromatography substrate, a magnetically active molecule, a gel forming and/or viscosity changing molecule, an immunoassay detectable molecule, a cell-based amplification detectable or a nucleic acid barcode, or any combinations thereof. Further provided herein is a method wherein said reporter comprises a fluorescent label. Further provided herein is a method wherein said fluorescent label is selected from a group consisting of a 5-carboxyfluorescein (5-FAM), a 7-amino-4-carbamoylmethylcoumarin (ACC), a 7-Amino-4-methylcoumarin (AMC), a 2-Aminobenzoyl (Abz), a Cy7, a Cy5, a Cy3 and a (5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid) (EDANS).


Further provided herein is a method wherein said molecule further comprises a fluorescent quencher. Further provided herein is a method wherein said fluorescent quencher is selected from the group consisting of BHQ0, BHQ1, BHQ2, BHQ3, BBQ650, ATTO 540Q, ATTO 580Q, ATTO 612Q, CPQ2, QSY-21, QSY-35, QSY-7, QSY-9, DABCYL (4-([4′-dimethylamino)phenyl]azo)benzoyl), Dnp (2,4-dinitrophenyl) and Eclipse. Further provided herein is a method wherein said fluorescent quencher is directly connected to said cleavable linker through a covalent bond.


Further provided herein is a method wherein said molecule further comprises a carrier. Further provided herein is a method wherein said carrier comprises a native, labeled or synthetic protein, a synthetic chemical polymer of precisely known chemical composition or with a distribution around a mean molecular weight, an oligonucleotide, a phosphorodiamidate morpholino oligomer (PMO), a foldamer, a lipid, a lipid micelle, a nanoparticle, a solid support made of polystyrene, polypropylene or any other type of plastic, or any combination thereof.


Further provided herein is a method wherein said subject is a mammal. Further provided herein is a method wherein said mammal is a human.


Further provided herein is a method wherein said reporter is linked to said cleavable linker through a self-immolative spacer. Further provided herein is a method wherein said self-immolative spacer is selected from the group consisting of a disulfide, a hetheroaminebifuncional disulfide, a thiol-based pirydazinediones, a p-aminebenzyloxycarbonyl, a dipeptide, a Gly-Pro (SEQ ID NO: 530), a L-Phe-Sar, a trans-cyclooctene tetrazine, a ortho Hydroxy-protected Aryl sulfate, a phosphoramidate-based spacer, a hydroxybenzyl, a trimethyl carbamate and a quinone methide-based spacer.


Further provided herein is a method wherein said detection comprises fluorescent detection, spectroscopic detection, mass spectrometry, immunological detection or imaging detection. Further provided herein is a method wherein said detection comprises fluorescent detection. Further provided herein is a method wherein said fluorescent detection is fluorescence resonance energy transfer (FRET).


Further provided herein is a method wherein said cleaved reporter comprises a precipitating fluorophore. Further provided herein is a method wherein said precipitating fluorophore comprises HPQ, Cl-HPQ, HTPQ, HBPQ, or HQPQ.


Further provided herein is a method wherein said body fluid sample is selected from the group consisting of blood, plasma, bone marrow fluid, lymphatic fluid, bile, amniotic fluid, mucosal fluid, saliva, urine, cerebrospinal fluid, spinal fluid, synovial fluid, semen, ductal aspirate, feces, stool, vaginal effluent, lachrymal fluid, tissue lysate and patient-derived cell line supernatant. Further provided herein is a method wherein said body fluid sample comprises a rinse fluid, a conditioning media or buffer, a swab viral transport media, a saline, a culture media, or a cell culture supernatant. Further provided herein is a method wherein said rinse fluid is selected from the group consisting of a mouthwash rinse, a bronchioalveolar rinse, a lavage fluid, a hair wash rinse, a nasal spray effluent, a swab of any bodily surface, orifice, organ structure or solid tumor biopsies applied to saline or any media or any derivatives thereof.


INCORPORATION BY REFERENCE

All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference.





BRIEF DESCRIPTION OF THE DRAWINGS

The novel features of the invention are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings (“FIGURE.” or “FIGURES.” herein), of which:



FIG. 1 shows a plurality of probes according to the current application. Each probe 101 includes a reporter 103, shown as a star in FIG. 1. The reporters 103, are linked to a cleavable linker 105, which is a cleavable substrate for an agent 107.



FIG. 2 shows cleavage of the reporter in a plurality of the probes. As shown, cleavage by the agent 107 of the cleavable linker 105 results in the reporters 103 being cleaved from the probe 101. Once cleaved, the cleaved reporters 203 can be detected and/or distinguished from un-cleaved reporters 103. The presence and detection of cleaved reporters 203 indicates that the agents 107 are present and active in a sample. In addition, the absence of an agent activity may be used for detection associated with a decrease in activity. The activity of the agents can be quantified based on, for example, the rate at which the cleavage reaction takes place or the amount of cleaved reporters in a sample or by other means such as a ratio of rates against an appropriate control or a ratio of cleaved reporters against an appropriate control.



FIG. 3 illustrates a method 301 of evaluating a biological condition in a subject using the probes 101.



FIG. 4 shows the selection of probes to use in a composition to analyze the activities of agents to analyze one or more particular, biological conditions or disease states. The activity of one or more agents may be associated with a biological condition or disease state. This may include the progression of a particular condition or state over time. Thus, to evaluate a biological condition or disease state in a subject, probes that can be cleaved by agents of interest are selected from the library for inclusion in a condition-specific panel 403. The selected probes 405 of the condition-specific panel are differentially labeled so that the activity of the predetermined proteases can be measured 305. The different probes 101, including those included in library 401, may include features that confer properties to the fragments that ensure accurate, multiplex detection of agent activity. Such properties include, for example improved cleavage, detection, solubility, stability, reproducibility, robustness and/or expanded compatibility with different types of reporter.



FIG. 5 shows a schematic of a probe 501 that includes a spacer 507, a solubility tag 509, a quencher and a covalent or non-covalent attachment site 511. The respective positions of these components can, in principle, be interconverted.



FIG. 6A-C shows cleavage of the probe. FIG. 6A shows that the probe 601 includes a fluorescent reporter 603 and a quencher 605. The probe 601 may also include a spacer 507, a solubility tag 509, and/or a covalent or non-covalent attachment site 511. FIG. 6B shows the cleavage process of two components probe. FIG. 6C shows the cleavage process of three components probe.



FIG. 7A-C shows reaction processes for HPQ fluorophore. FIG. 7A shows a probe 701 with an auto-immolative spacer 705 and precipitating fluorescent reporter 703. The spacer 705 connects the precipitating fluorophore reporter to an exopeptidase substrate 707, which is surrounded by the rectangle for clarity. A specific, predetermined exopeptidase cleaves the exopeptidase substrate 707. As a result, the auto-immolative spacer 705 dissociates from the precipitating fluorophore reporter 703. This allows establishment of a particular hydrogen bond 709 in the reporter 703, such that it enters a solid state, precipitates from the fluid sample, and provides an intense fluorescent signal. FIG. 7B shows de detailed process. FIG. 7C shows the reaction process with both endopeptidase and exopeptidase.



FIG. 8 shows a method using a probe 801 with an auto-immolative spacer 807, precipitating or non precipitating fluorescent reporter 805, and an enzyme/protease substrate 809 cleaved by a predetermined enzyme/endoprotease 803. The probe includes an enzyme/protease substrate 809 that is cleaved by two predetermined enzymes/proteases. The first of these enzymes/proteases, is the enzyme/endoprotease 803 of interest in the sample. The enzyme/endoprotease 803 in the fluid sample cleaves the enzyme/protease substrate 809. However, because 803, cannot cleave completely/the terminal or penultimate amino acids in the protease substrate from the spacer 807. Thus, a predetermined exopeptidase/enzyme 811 is introduced to the sample. The exopeptidase/enzyme can be spiked into the fluid sample, before, after, or during incubation with the endoprotease/enzyme 803. The enzyme/protease substrate 805 is engineered such that cleavage by the enzyme/endoprotease 803 results in a second enzyme/protease substrate 813 that can be cleaved by the predetermined enzyme/exopeptidase 811. Cleavage by 811 causes the spacer 807 to dissociate from the precipitating/non-precipitating fluorophore reporter 805, such the reporter 805 provides an intense fluorescent signal.



FIG. 9 shows the progression of NASH.



FIG. 10 shows in vivo probes used to detect protease activity.



FIG. 11 shows the protease activities measured using the in vivo probes.



FIG. 12 outlines an experiment of present application.



FIG. 13 outlines an experiment of present application.



FIG. 14 shows that the probes can accurately detect and differentiate between samples from patients diagnosed with NASH via liver biopsy and healthy patient samples when encountering NASH-related proteases in mice K2EDTA plasma.



FIG. 15A-B provide experimental results showing that a specific peptide linker of the present application can differentiate between NASH-related protease activity in healthy mice and NASH+ samples from K2EDTA mice plasma. FIG. 15A shows the results from healthy samples. FIG. 15B shows results from NASH+ samples.



FIG. 16 provides experimental results comparing the ex vivo probes and their ability to distinguish between NASH (CDHFD) samples (the right data point) and healthy (CD) samples (the left data point).



FIG. 17 provides raw experimental results showing that the measured rate of fluorescence increase for Probe #492 can be ascribed to protease activity and to NASH disease in K2EDTA mice plasma The average rate of fluorescence increase over n=10 samples matches pooled plasma (n=10) increase of fluorescence in both disease and healthy conditions.



FIG. 18 provides experimental results showing that the measure rate of fluorescence increase for Probe #102 can be ascribed to protease activity and to NASH disease in K2EDTA mice plasma. The average rate of fluorescence increase over n=10 samples matches pooled plasma (n=10) increase of fluorescence in both disease and healthy conditions.



FIG. 19A-B provides experimental results showing that activity, not abundance, is responsible for determination of disease-based protease activity differences in K2EDTA mouse plasma samples.



FIG. 19A shows the results of testing for protease abundance levels and FIG. 19B shows the results of testing for protease activity levels.



FIG. 20 outlines an experimental design of the present application.



FIG. 21A-F provide experimental results showing that several probes can differentiate among healthy K2EDTA plasma samples (left), regression samples (center), and NASH samples (right). FIG. 21A shows the results of Probe #428, FIG. 21B shows the results of Probe #520, FIG. 21C shows the results of Probe #96, FIG. 21D shows the results of Probe #102, FIG. 21E shows the results of Probe #492, and FIG. 21F shows the results of Probe #647.



FIG. 22 provides experimental results showing the probes can distinguish between healthy and the JO2 mouse model of fulminant hepatitis samples ex vivo. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis.



FIG. 23 provides experimental results showing the probes can distinguish between healthy and fulminant hepatitis samples in vivo in a mice model. +/++ group denotes mild hepatitis symptoms and +++/++++ group denotes fulminant hepatitis based on physio-pathological examination of mice. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis.



FIG. 24 shows that peptide fragments can distinguish between two different preclinical models of liver disease due to their distinct biological mechanisms.



FIG. 25 outlines an experimental design of the present application.



FIG. 26 provides experimental results showing the probes can distinguish between healthy, Obese and NASH human samples.



FIG. 27 provides experimental results that show reproducibility among independent sample cohorts with various collection dates, collection protocols, shipment etc.



FIG. 28 provides experimental results showing the peptide fragments can distinguish between different stages of NASH disease progression in specific assay conditions.



FIG. 29 provides experimental results showing the multiplicity of the peptide fragments able to distinguish between NASH and Healthy human K2EDTA plasma.



FIG. 30A-F provide experimental results demonstrating the association of specific proteases in the detection of disease-specific activity differences in NASH samples in mice K2EDTA plasma. FIG. 30A shows the results when testing with a pan-protease inhibitor. FIG. 30B shows the results when testing with a cysteine protease family inhibitor. FIG. 30C shows the results when testing with a cathepsin family inhibitor. FIG. 30D shows the results when testing with a CTSB specific inhibitor.



FIG. 30E shows the results when testing with a CTSK specific inhibitor. FIG. 30F shows the results when testing with a CTSL specific inhibitor. These results show that this substrate is cleaved by CTSL.



FIG. 31A-B provides experimental results showing that two common promiscuous proteases abundant in plasma are not responsible for determination of disease-based protease activity differences in NASH samples in K2EDTA mice plasma. FIG. 31A shows the results of testing with a trypsin specific inhibitor and FIG. 31B shows the results when testing with a thrombin specific inhibitor.



FIG. 32A-B provides experimental results showing that activity, not abundance, is responsible for determination of disease-based protease activity differences in human samples. FIG. 32A shows the results of testing pooled samples of healthy and NASH plasma when comparing protease activity.



FIG. 32B shows the quantitation ratio for protease activity between healthy and NASH samples.



FIG. 33A-B shows that although Cathepsin-L is equally abundant in both healthy and NASH human samples, the differences in its activity levels allow for the differentiation between healthy and NASH samples. FIG. 33A shows the results of testing for CTSL abundance levels and FIG. 33B shows that testing for CTSL activity levels is superior to testing for CTSL abundance.



FIG. 34A-B provides experimental evidence that the probes can detect both host response and presence of the COVID virus in plasma under two different conditions of plasma collection. FIG. 34A shows the results from the K2EDTA plasma cohort while FIG. 34B shows the results from the LiHeparin plasma cohort. Probe #18 is a Neutrophil elastase substrate. Probe #409 is a SARS-COV2 3C protease. Probe #462 is a MMP8 substrate. Probe #84 is a Furin substrate. Probe #26 is a Cathepsin K/B, Trypsin, Thrombin, Tryptase substrate.



FIG. 35 provides experimental data that the probes can differentiate between healthy swab samples and COVID swab samples.



FIG. 36A-B provides experimental data showing that 3C1 protease from SARS-COV2 can be detected when spiked in saliva or swab samples. FIG. 36A shows the results from saliva samples while FIG. 36B shows the results from swab samples conditioned in VTM (Viral Transport Media containing up to 10% FBS).



FIG. 37 shows several probes that are capable of differentiating between healthy and COVID samples.



FIG. 38A provides experimental evidence that the Probe #647 can detect the activity of COVID-related proteases to differentiate between healthy and COVID pooled swab samples conditioned in saline. FIG. 38B shows that there are significant differences (p=0.029) between COVID+ (n=18) and COVID− (n-19) samples. FIG. 38C shows the adjusted RFU across timepoints for COVID+ (7 samples were active) and COVID− (1 sample was active) samples.



FIG. 39A-B provides experimental evidence that Granzyme B, a protease linked to other autoimmune diseases, is the protease that allows Probe #647 to differentiate between healthy and COVID samples. FIG. 39A shows the results of inhibition experiments involving Granzyme B while FIG. 39B shows the results of inhibition experiments involving caspases. Differential protease activity is more sensitive to the GzmB specific inhibitor than the caspase inhibitor, implicating GzmB, a hallmark of T-cell activity, in the disease signal detected in swabs.



FIG. 40 shows a paper strip test capable of monitoring Granzyme B activity.



FIG. 41A-B provides experimental evidence showing that the peptide fragments can distinguish between healthy and pancreatic ductal adenocarcinoma (PDAC) samples. FIG. 41A shows the results of first set of experiments, while FIG. 41B shows the results of second set of experiments.



FIG. 42 provides experimental evidence showing that the peptide fragments can distinguish between healthy samples, PDAC samples, and pancreatitis samples.



FIG. 43 shows a schematic diagram for detection of Chlorination and peroxidation activity of MPO using the EnzChek® Myeloperoxidase Activity Assay Kit. AH represents the nonfluorescent Amplex® UltraRed substrate, and A represents its fluorescent oxidation product. Hydrogen peroxide converts MPO to MPO-I and MPO is inactive without the presence of hydrogen peroxide. Amplex® UltraRed is then oxidized by MPO-I and creates the fluorescent oxidation product A which can be read at Ex/Em=530/590.



FIG. 44A-C shows the results for detecting peroxidases. FIG. 44A shows that MPO activities are different between healthy mice and mice with NASH. FIG. 44B shows that MPO activities are different between mice fed on a standard ChowDiet (CD), mice feed on a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD). FIG. 44C shows that MPO activities are different between healthy human subject and subjects with rheumatoid arthritis.



FIG. 45A-B shows the pooled results of spiked recombinant protease in human plasma using resorufin oleate as substrate. FIG. 46A shows result of 3 recombinant enzymes—carboxylesterase 1, phospholipase A2 and lipoprotein lipase. FIG. 46B shows the result of various concentrations of lipoprotein lipase.



FIG. 46A-C shows general designs of the exemplary cleavable linkers for FRET substrates.



FIG. 46A shows general designs for endopeptidase, aminopeptidase and carboxypeptidase substrates.



FIG. 46B shows an example that reporter and quencher can be inverted. FIG. 46C shows the generalized substrate designs for aminopeptidase and carboxypeptidase.





DETAILED DESCRIPTION

Provided herein are methods comprising contacting a body fluid sample from a subject with a molecule ex vivo. In some embodiments, the molecule comprises a cleavable linker and a reporter, and the cleavable linker is cleaved by an agent from the body fluid, releasing the reporter from the molecule. In some embodiments, the method further comprises detecting a rate of formation or an amount of the released reporter. In some embodiments, the rate of formation or amount of the released report is significantly different from a healthy subject. In some embodiments, the body fluid may be plasma. In some embodiments, the method further comprises determining a disease or condition of the subject based on the detection.


In one aspect, the body fluid sample is contacted by a second molecule with a second cleavable linker and a second reporter. In some embodiments, the second cleavable linker is cleaved by a second agent from the body fluid, releasing the second reporter from the second molecule. In some embodiments, the method further comprises detecting a rate of formation or an amount of the second released reporter. In some embodiments, the method further comprises determining a disease or condition of the subject based on the detection of the first released reporter and the detection of the second released reporter. In some embodiments, the method described herein can be used in a multiplexed format, such that a single body fluid sample can be used to ascertain the activity of multiple, select agents. This allows diagnostic panels to be created for specific pathologies and conditions, which leverage the activity of multiple agents to provide a more complete and accurate assessment of a certain condition. These panels can be used to correlate the activity of multiple agents with a particular condition or disease-state. These signatures can be saved, for example, in a database and used to assess the conditions or disease-state for subsequent individuals assessed by a particular protease activity panel. In some embodiments, a classification tool is used in the analysis to differentiate between healthy and diseased patients, or between discrete stages of disease. The classification tool may be supervised Machine Learning classification algorithms including but not limited to Logistic Regression, Naive Bayes, Support Vector Machine, Random Forest, Gradient Boosting or Neural Networks. Furthermore, if the modeled variable is continuous in nature (e.g. tumor volume), one could use continuous regression approaches such as Ridge Regression, Kernel Ridge Regression, or Support Vector Regression. These algorithms would operate on the multi-dimensional feature space defined by the measurements of multiple probes (or a mathematical function of those measurements such as probe ratios) in order to learn the relationship between probe measurements and disease status. Finally, one could combine probe measurements with clinical variables such as age, gender, or patients” comorbid status. In that case, one could either incorporate clinical features in the classifier directly or, alternatively, learn a second-order classifier which combines a probe-only prediction with clinical features to produce a result that is calibrated for those variables.


In some embodiments, the disease or condition may be a certain fibrosis stage or a certain nonalcoholic fatty liver disease activity score (NAS) of Non-alcoholic steatohepatitis (NASH). In some embodiments, the disease or condition may be a liver disease, a cancer, an organ transplant rejection, an infectious disease, an allergic disease, an autoimmunity and a chronic inflammation.


In another aspect, the methods described herein comprises ex vivo, multiplex detection of enzyme activity to diagnose and monitor pathologies and treatments in a subject. This enzyme activity can be used to diagnose and monitor a disease and condition in an internal organ of the subject.


Detection Probe/Molecule


Determination of the disease or condition is based on the rate of formation or amount of the released reporter detected in the sample. A probe/molecule is introduced to the body fluid samples. The probe/molecule comprises a cleavable linker and a reporter, and an agent of from the body fluid cleave the cleavable linker, releasing a cleaved reporter. The probe/molecule may have any structure that can fulfill this function. In some embodiments, the reporter may be covalently linked to a cleavable linker. In some embodiments, the reporter may be a fluorescent label, a mass tag, a chromophore, an electrochemically active molecule, a bio-Layer interferometry or surface plasmon resonance detectable molecule, a precipitating substance, a mass spectrometry and liquid chromatography substrate (including size exclusion, reverse phase, isoelectric point, etc.), a magnetically active molecule, a gel forming and/or viscosity changing molecule, an immunoassay detectable molecule, a cell-based amplification detectable molecule, a nucleic acid barcode, or any combinations thereof.


In some embodiments, the reporter may be a fluorescent label and the molecule also comprises a quencher. In some embodiments, the quencher is covalently linked to the cleavable linker. In some embodiments an internally quenched fluorophore is linked to the cleavable linker. In some embodiments, the molecule further comprises a self-immolative spacer. In some other embodiments, the molecule further comprises a carrier.


Cleavable Linker


In some aspects, the probe/molecule described herein comprises a cleavable linker. The cleavable linker as described herein may be in any structure that is capable of being cleaved by an agent. In some embodiments, the cleavable linker may be a peptide, a carbohydrate, a nucleic acid, a lipid, an ester, a glycoside, a phospholipid, a phosphodiester, a nucleophile/base sensitive linker, a reduction sensitive linker, an electrophile/acid sensitive linker, a metal cleavable linker, an oxidation sensitive linker, an auto-immolable linker (three component probe=enzyme substrate+linker+reporter) or a combination thereof. In some embodiments, the reporter can be in an inactive form and under disease activity becomes detectable. Geoffray Leriche, Louise Chisholm, Alain Wagner, Cleavable linkers in chemical biology, Bioorganic & Medicinal Chemistry, Volume 20, Issue 2, 2012, Pages 571-582, ISSN 0968-0896, https://doi.org/10.1016/j.bmc.2011.07.048.


Cross-linking agents aim to form a covalent bond between two spatially adjacent residues within one or two polymer chains. To identify protein binding partners, the cross-linking agents need to be able to detect and stabilize transient interactions. The crosslinking agents frequently form covalent links between lysine or cysteine residues in the proteins. Alternatively, the cross-linking agent can be photoreactive. Cross-linking cleavable linkers can be used to distinguish between inter- and intra-protein interactions of receptors, signaling cascades, and the structure of multi-protein complexes.


In some embodiments, the cleavable linker may be a peptide. The core structure of a peptide linker sometimes comprises of either a di-peptide or a tetra-peptide that is recognized and cleaved by lysosomal enzymes. Proteases (also called peptidases) catalyze the breakdown of peptide bonds by hydrolysis, and is restricted to a specific sequence of amino acids recognizable by the proteases. Commonly used proteases comprise pepsin, trypsin or chymotrypsin. Since proteases have key roles in many diseases, peptide linkers are widely used in drug release systems or in diagnostic tools. In some embodiments, the peptide linkers comprise a short peptide sequence. In some embodiments, the peptide linkers may include at least one non-naturally occurring amino acid.


In some embodiments, the peptide linkers may be less than about 20 amino acids in length. In some embodiments, the peptide linkers may be between 10 and 100 amino acids in length. In some embodiments, the peptide linkers may be 1 to 5, 1 to 10, 1 to 20, 1 to 30, 1 to 50, 1 to 70, 1 to 90, 1 to 100, 5 to 10, 5 to 20, 5 to 30, 5 to 50, 5 to 70, 5 to 90, 5 to 100, 10 to 20, 10 to 30, 10 to 50, 10 to 70, 10 to 90, 10 to 100, 20 to 30, 20 to 50, 20 to 70, 20 to 90, 20 to 100, 30 to 50, 30 to 70, 30 to 90, 30 to 100, 50 to 70, 50 to 90, 50 to 100, 70 to 90, 70 to 100, or 90 to 100 amino acids in length.









TABLE 1







Exemplary sequences for peptide linkers and corresponding probe construct designs











SEQ



SEQ


ID

Exemplary

ID


NO
Sequence
probe name
Exemplary probe construct
NO 





1
SGRSG
Probe #1
5-FAM-GSGRSGGK(CPQ2)-PEG2-kk-GC
678





2
PGPREG
Probe #2
5-FAM-GPGPREGGK(CPQ2)-PEG2-kk-GC
679





3
IEPDSGSQ
Probe #3
5-FAM-GIEPDSGSQGK(CPQ2)-PEG2-kk-GC
680





4
WADSSMES
Probe #4
5-FAM-GWADSSMESGK(CPQ2)-PEG2-kk-
681





GC






5
PTSY
Probe #5
5-FAM-GPTSYGK(CPQ2)-PEG2-kk-GC
682





6
YRFK
Probe #6
5-FAM-GYRFKGK(CPQ2)-PEG2-kk-GC
683





7
KVPL
Probe #7
5-FAM-GKVPLGK(CPQ2)-PEG2-kk-GC
684





8
VDVAD
Probe #8
5-FAM-GVDVADGK(CPQ2)-PEG2-kk-GC
685





9
LETD
Probe #9
5-FAM-GLETDGK(CPQ2)-PEG2-kk-GC
686





10
LEHD
Probe #10
5-FAM-GLEHDGK(CPQ2)-PEG2-kk-GC
687





11
REQD
Probe #11
5-FAM-GREQDGK(CPQ2)-PEG2-kk-GC
688





12
DEVD
Probe #12
5-FAM-GDEVDGK(CPQ2)-PEG2-kk-GC
689





13
VEID
Probe #13
5-FAM-GVEIDGK(CPQ2)-PEG2-kk-GC
690





14
VQVDGW
Probe #14
5-FAM-GVQVDGWGK(CPQ2)-PEG2-kk-GC
691





15
YEVDGW
Probe #15
5-FAM-GYEVDGWGK(CPQ2)-PEG2-kk-GC
692





16
LEVD
Probe #16
5-FAM-GLEVDGK(CPQ2)-PEG2-kk-GC
693





17
IEVE
Probe #17
5-FAM-GIEVEGK(CPQ2)-PEG2-kk-GC
694





18
AAPV
Probe #18
5-FAM-GAAPVGK(CPQ2)-PEG2-kk-GC
695





19
FFKF
Probe #19
5-FAM-GFFKFGK(CPQ2)-PEG2-kk-GC
696





20
GRRGKGG
Probe #20
5-FAM-GGRRGKGGGK(CPQ2)-PEG2-kk-
697





GC






21
VKKR
Probe #21
5-FAM-GVKKRGK(CPQ2)-PEG2-kk-GC
698





22
FAAF(NO2)
Probe #22
5-FAM-GFAAF(NO2)FVLGK(CPQ2)-PEG2-
699



FVL

kk-GC






23
VVR
Probe #23
5-FAM-GVVRGK(CPQ2)-PEG2-kk-GC
700





24
KQKLR
Probe #24
5-FAM-GKQKLRGK(CPQ2)-PEG2-kk-GC
701





25
RPPGFSAF
Probe #25
5-FAM-GRPPGFSAFGK(CPQ2)-PEG2-kk-
702





GC






26
GPR
Probe #26
5-FAM-GGPRGK(CPQ2)-PEG2-kk-GC
703





27
FR
Probe #27
5-FAM-GFRGK(CPQ2)-PEG2-kk-GC
704





28
LPLGL
Probe #28
5-FAM-GLPLGLGK(CPQ2)-PEG2-kk-GC
705





29
KPLGL
Probe #29
5-FAM-GKPLGLGK(CPQ2)-PEG2-kk-GC
706





30
(Gaba)PQGLE
Probe #30
5-FAM-G(Gaba)PQGLEGK(CPQ2)-PEG2-
707





kk-GC






31
PKPLAL
Probe #31
5-FAM-GPKPLALGK(CPQ2)-PEG2-kk-GC
708





32
GPSGIHV
Probe #32
5-FAM-GGPSGIHVGK(CPQ2)-PEG2-kk-GC
709





33
WAHRTTFYRR
Probe #33
5-FAM-GWAHRTTFYRRGAGK(CPQ2)-
710



GA

PEG2-kk-GC






34
WKLRSSKQ
Probe #34
5-FAM-GWKLRSSKQGK(CPQ2)-PEG2-kk-
711





GC






35
PFR
Probe #35
5-FAM-GPFRGK(CPQ2)-PEG2-kk-GC
712





36
SYRIF
Probe #36
5-FAM-GSYRIFGK(CPQ2)-PEG2-kk-GC
713





37
RPY
Probe #37
5-FAM-GRPYGK(CPQ2)-PEG2-kk-GC
714





38
TAFRSAYG
Probe #38
5-FAM-GTAFRSAYGGK(CPQ2)-PEG2-kk-
715





GC






39
WAAFRFSQA
Probe #39
5-FAM-GWAAFRFSQAGK(CPQ2)-PEG2-kk-
716





GC






40
VPR
Probe #40
5-FAM-GVPRGK(CPQ2)-PEG2-kk-GC
717





41
G
Probe #41
5-FAM-GGK(CPQ2)-PEG2-kk-GC
718





42
KLRSSKQ
Probe #42
5-FAM-GKLRSSK0GK(CPQ2)-PEG2-kk-GC
719





43
YASR
Probe #43
5-FAM-GYASRGK(CPQ2)-PEG2-kk-GC
720





44
RFAQAQQQLP
Probe #44
5-FAM-GRTAQAQQQLPGK(CPQ2)-PEG2-
721





kk-GC






45
KPAKFFRL
Probe #45
5-FAM-GKPAKFFRLGK(CPQ2)-PEG2-kk-
722





GC






46
PRAAA(hF)TSP
Probe #46
5-FAM-GPRAAA(hF)TSPGK(CPQ2)-PEG2-
723





kk-GC






47
VGPQRFSGAP
Probe #47
5-FAM-GVGPQRFSGAPGK(CPQ2)-PEG2-
724





kk-GC






48
FFLAQA(hF)RS
Probe #48
5-FAM-GFFLAQA(hF)RSGK(CPQ2>PEG2-
725





kk-GC






49
PLAQAV
Probe #49
5-FAM-GPLAQAVGK(CPQ2)-PEG2-kk-GC
726





50
RTAAVFRP
Probe #50
5-FAM-GRTAAVFRPGK(CPQ2)-PEG2-kk-
727





GC






51
DVQEFRGVTA
Probe #51
5-FAM-GDVQEFRGVTAVIRGK(CPQ2)-
728



VIR

PEG2-kk-GC






52
TEGEARGSVI
Probe #52
5-FAM-GTEGEARGSVIGK(CPQ2)-PEG2-
729





kk-GC






53
l-TR
Probe #53
5-FAM-G-l-TRGK(CPQ2)-PEG2-kk-GC
730





54
PLFAERK
Probe #54
5-FAM-GPLFAERKGK(CPQ2)-PEG2-kk-GC
731





55
LLVY
Probe #55
5-FAM-GLLVYGK(CPQ2)-PEG2-kk-GC
732





56
QQKRKIVL
Probe #56
5-FAM-GQQKRKIVLGK(CPQ2)-PEG2-kk-
733





GC






57
ASHLGLAR
Probe #57
5-FAM-GASHLGLARGK(CPQ2)-PEG2-kk-
734





GC






58
LPSRSSKI
Probe #58
5-FAM-GLPSRSSKIGK(CPQ2)-PEG2-kk-GC
735





59
STGRNGFK
Probe #59
5-FAM-GSTGRNGFKGK(CPQ2)-PEG2-kk-
736





GC






60
SLLRSEET
Probe #60
5-FAM-GSLLRSEETGK(CPQ2)-PEG2-kk-GC
737





61
HRGRTLEI
Probe #61
5-FAM-GHRGRTLEIGK(CPQ2)-PEG2-kk-
738





GC






62
YLGRSYKV
Probe #62
5-FAM-GYLGRSYKVGK(CPQ2)-PEG2-kk-
739





GC






63
EKQRIIGG
Probe #63
5-FAM-GEKQRIIGGGK(CPQ2)-PEG2-kk-GC
740





64
QRQRIIGG
Probe #64
5-FAM-GQRQRIIGGGK(CPQ2)-PEG2-kk-
741





GC






65
LQRIYK
Probe #65
5-FAM-GLQRIYKGK(CPQ2)-PEG2-kk-GC
742





66
SLGRKIQI
Probe #66
5-FAM-GSLGRKIQIGK(CPQ2)-PEG2-kk-GC
743





67
HAAPRSADIQI
Probe #67
5-FAM-GHAAPRSADIQIDIGK(CPQ2)-
744



DI

PEG2-kk-GC






68
FGR
Probe #68
5-FAM-GFGRGK(CPQ2)-PEG2-kk-GC
745





69
SLGR
Probe #69
5-FAM-GSLGRGK(CPQ2)-PEG2-kk-GC
746





70
GLQR
Probe #70
5-FAM-GGL0RGK(CPQ2)-PEG2-kk-GC
747





71
SVARTLLV
Probe #71
5-FAM-GSVARTLLVGK(CPQ2)-PEG2-kk-
748





GC






72
GRIFG
Probe #72
5-FAM-GGRIFGGK(CPQ2)-PEG2-kk-GC
749





73
APK
Probe #73
5-FAM-GAPKGK(CPQ2)-PEG2-kk-GC
750





74
GFSPY
Probe #74
5-FAM-GGFSPYGK(CPQ2)-PEG2-kk-GC
751





75
WELRHAGH
Probe #75
5-FAM-GWELRHAGHGK(CPQ2)-PEG2-kk-
752





GC






76
RQSRIVGGE
Probe #76
5-FAM-GRQSRIVGGEGK(CPQ2)-PEG2-kk-
753





GC






77
EQAVYQTI
Probe #77
5-FAM-GEQAVYQTIGK(CPQ2)-PEG2-kk-
754





GC






78
VAYSGENTFG
Probe #78
5-FAM-GVAYSGENTFGFGK(CPQ2)-PEG2-
755



F

kk-GC






79
GGR
Probe #79
5-FAM-GGGRGK(CPQ2)-PEG2-kk-GC
756





80
ATAD
Probe #80
5-FAM-GATADGK(CPQ2)-PEG2-kk-GC
757





81
RPLESNAV
Probe #81
5-FAM-GRPLESNAVGK(CPQ2)-PEG2-kk-
758





GC






82
RPLGLAR
Probe #82
5-FAM-GRPLGLARGK(CPQ2)-PEG2-kk-GC
759





83
AAFF
Probe #83
5-FAM-GAAFFGK(CPQ2)-PEG2-kk-GC
760





84
RVKRGLA
Probe #84
5-FAM-GRVKRGLAGK(CPQ2)-PEG2-kk-GC
761





85
AAL
Probe #85
5-FAM-GAALGK(CPQ2)-PEG2-kk-GC
762





86
CGGmeGVndne
Probe #86
5-FAM-CGGmeGVndneeGFFsArGK(CPQ2)
763



eGFFsAr








87
GPQGIWGQ
Probe #87
5FAM-GGPOGIWGOK(CPQ2)-PEG2-C
764





88
GLVPRGS
Probe #88
5FAM-GGLVPRGSGK(CPQ2)-PEG2-C
765





89
GPVGLI
Probe #89
5FAM-GGPVGLIGK(CPQ2)-PEG2-C
766





90
GPWGIWGQ
Probe #90
5FAM-GGPWGIWGQGK(CPQ2)-PEG2-C
767





91
GPVPLSLVM
Probe #91
5FAM-GGPVPLSLVMK(CPQ2)-PEG2-C
768





92
Gf-Pip-RSGG
Probe #92
5FAM-GGf-Pip-RSGGGK(CPQ2)-PEG2-C
769





93
PLGMRG
Probe #93
5FAM-GGf-Pip-KSGGGK(CPQ2)-PEG2-C
770





94
PLGMRG
Probe #94
(FAM)-GPLGMRGG-K(CPQ2)-PEG2-k-GC
771





95
P-(Cha)-G-
Probe #95
(FAM)-GP-(Cha)-G-Cys(Me)-HAG-K(CPQ2)-
772



CvsfMel-HA

PEG2-kk-GC






96
RPLALWESQ
Probe #96
(FAM)-GRPLALWESQG-K(CPQ2)-PEG2-k-
773





GC






97
SGKGPRQITA
Probe #97
(FAM)-SGKGPR0ITA-K(CPQ2)-PEG2-k-GC
774





98
SGPLFYSVTA
Probe #98
(FAM)-SGPLFYSVTA-K(CPQ2)-PEG2-kk-
775





GC






99
SGRIFLRTA
Probe #99
(FAM)-SGRIFLRTA-K(CPQ2)-PEG2-GC
776





100
SGRSENIRTA
Probe #100
(FAM)-SGRSENIRTA-K(CPQ2)-PEG2-GC
777





101
GSGGS
Probe #101
(FAM)-GGSGGS-K(CPQ2)-PEG2-kk-GC
778





102
KPILFFRLKG
Probe #102
(FAM)-GKPILFFRLKG-K(CPQ2)-PEG2-kk-
779





GC






103
AWESR(Nle)
Probe #103
(FAM)-GAWESR(Nle)GK(CPQ2)-NH2
780





104
NEKSG(Nle)
Probe #104
(FAM)-GNEKSG(Nle)GK(CPQ2)-NH2
781





105
NATIVY
Probe #105
(FAM)-GNATIVYGK(CPQ2)-PEG2-k-NH2
782





106
DPFVVS
Probe #106
(FAM)-GDPFVVSGK(CPQ2)-PEG2-k-NH2
783





107
FH(Nle)FTK
Probe #107
(FAM)-GFH(Nle)FTKGK(CPQ2)-PEG2-k-
784





NH2






108
(Nle)NWHKH
Probe #108
(FAM)-G(Nle)NWHKHGK(CPQ2)-NH2
785





109
FARRWG
Probe #109
(FAM)-GFARRWGGK(CPQ2)-PEG2-k-NH2
786





110
PGKWSK
Probe #110
(FAM)-GPGKWSKGK(CPQ2)-PEG2-k-NH2
787





111
YEEAQP
Probe #111
(FAM)-GYEEAQPGK(CPQ2)-PEG2-k-NH2
788





112
YGAIKK
Probe #112
(FAM)-GYGAIKKGK(CPQ2)-PEG2-k-NH2
789





113
TS(Nle)EGY
Probe #113
(FAM)-GTS(Nle)EGYGK(CPQ2)-PEG2-k
790





114
PNNFGS
Probe #114
(FAM)-GPNNFGSGK(CPQ2)-PEG2-k-NH2
791





115
EDTRNT
Probe #115
(FAM)-GEDTRNTGK(CPQ2)-NH2
792





116
KDLEQS
Probe #116
(FAM)-GKDLEQSGK(CPQ2)-NH2
793





117
AALHND
Probe #117
(FAM)-GAALHNDGK(CPQ2)-PEG2-kk-NH2
794





118
ADSFFK
Probe #118
(FAM)-GADSFFKGK(CPQ2)-NH2
795





119
ITFWRA
Probe #119
(FAM)-GITFWRAGK(CPQ2)-NH2
796





120
LSD(Nle)RL
Probe #120
(FAM)-GLSD(Nle)RLGK(CPQ2)-NH2
797





121
EVGWTY
Probe #121
(FAM)-GEVGWTYGK(CPQ2)-PEG2-k-NH2
798





122
IAFRQ(Nle)
Probe #122
(FAM)-GIAFRO(Nle)GK(CPQ2)-NH2
799





123
YNIHT(Nle)
Probe #123
(FAM)-GYNIHT(Nle)GK(CPQ2)-PEG2-kk-
800





NH2






124
(Nle)LWANH
Probe #124
(FAM)-G(Nle)LWANHGK(CPQ2)-PEG2-kk-
801





NH2






125
LYSVQV
Probe #125
(FAM)-GLYSVQVGK(CPQ2)-PEG2-k-NH2
802





126
SHI(Nle)SN
Probe #126
(FAM)-GSHI(Nle)SNGK(CPQ2)-PEG2-kk-
803





NH2






127
KLLIDV
Probe #127
(FAM)-GKLLIDVGK(CPQ2)-NH2
804





128
E(Nle)GVFD
Probe #128
(FAM)-GE(Nle)GVFDGK(CPQ2)-PEG2-k-
805





NH2






129
HQAYTL
Probe #129
(FAM)-GHQAYTLGK(CPQ2)-PEG2-kk-NH2
806





130
YVRKIQ
Probe #130
(FAM)-GYVRKIOGK(CPQ2)-PEG2-k-NH2
807





131
DRENSP
Probe #131
(FAM)-GDRENSPGK(CPQ2)-NH2
808





132
KYDKPR
Probe #132
(FAM)-GKYDKPRGK(CPQ2)-NH2
809





133
RPWKQL
Probe #133
(FAM)-GRPWKQLGK(CPQ2)-PEG2-k-NH2
810





134
APLQRY
Probe #134
(FAM)-GAPLQRYGK(CPQ2)-NH2
811





135
YQGQK(Nle)
Probe #135
(FAM)-GYqGqK(Nle)GK(CPQ2)-NH2
812





136
GRISSI
Probe #136
(FAM)-GGRISSIGK(CPQ2)-NH2
813





137
HSLTNV
Probe #137
(FAM)-GHSLTNVGK(CPQ2)-PEG2-kk-NH2
814





138
EWDFPE
Probe #138
(FAM)-GEWDFPEGK(CPQ2)-PEG2-k-NH2
815





139
YLA(Nle)DG
Probe #139
(FAM)-GYLA(Nle)DGGK(CPQ2)-PEG2-k-
816





NH2






140
FIY(Nle)PT
Probe #140
(FAM)-GFIY(Nle)PTGK(CPQ2)-PEG2-k-NH2
817





141
GHETWV
Probe #141
(FAM)-GGHETWVGK(CPQ2)-PEG2-kk-NH2
818





142
DYIGDE
Probe #142
(FAM)-GDYIGDEGK(CPQ2)-PEG2-k-NH2
819





143
AGTAHP
Probe #143
(FAMl-GAGTAHPGK(CPQ2)-PEG2-kk-NH2
820





144
V(Nle)TEIW
Probe #144
(FAM)-GV(Nle)TEIWGK(CPQ2)-PEG2-k-
821





NH2






145
PDDWQN
Probe #145
(FAM)-GPDDWONGK(CPQ2)-PEG2-k-NH2
822





146
GLNQEY
Probe #146
(FAM)-GGLNqEYGK(CPQ2)-PEG2-k-NH2
823





147
YRDAVA
Probe #147
(FAM)-GYRDAVAGK(CPQ2)-NH2
824





148
TGPKGN
Probe #148
(FAM)-GTGPKGNGK(CPQ2)-NH2
825





149
DHVPQI
Probe #149
(FAM)-GDHVPOIGK(CPQ2)-PEG2-kk-NH2
826





150
NKEPIL
Probe #150
(FAM)-GNKEPILGK(CPQ2)-NH2
827





151
VWN(Nle)VH
Probe #151
(FAM)-GVWN(Nle)VHGK(CPQ2)-PEG2-kk-
828





NH2






152
PVIIEH
Probe #152
(FAM)-GPVIIEHGK(CPQ2)-PEG2-kk-NH2
829





153
FOTDNL
Probe #153
(FAM)-GFQTDNLGK(CPQ2)-PEG2-k-NH2
830





154
RF(Nle)HGI
Probe #154
(FAM)-GRF(Nle)HGIGK(CPQ2)-PEG2-k-
831





NH2






155
YAERTT
Probe #155
(FAM)-GYAERTTGK(CPQ2)-NH2
832





156
NRGELP
Probe #156
(FAM)-GNRGELPGK(CPQ2)-NH2
833





157
HHYFNY
Probe #157
(FAM)-GHHYFNYGK(CPQ2)-PEG2-k-NH2
834





158
STPYYH
Probe #158
(FAM)-GSTPYYHGK(CPQ2)-PEG2-kk-NH2
835





159
WFYPSA
Probe #159
(FAM)-GWFYPSAGK(CPQ2)-PEG2-k-NH2
836





160
SEFLFS
Probe #160
(FAM)-GSEFLFSGK(CPQ2)-PEG2-k-NH2
837





161
WYKTQY
Probe #161
(FAM)-GWYKTOYGK(CPQ2)-NH2
838





162
VTHLKV
Probe #162
(FAM)-GVTHLKVGK(CPQ2)-PEG2-k-NH2
839





163
INGGFS
Probe #163
(FAM)-GINGGFSGK(CPQ2)-PEG2-k-NH2
840





164
TVLGLD
Probe #164
(FAM)-GTVLGLDGK(CPQ2)-PEG2-k-NH2
841





165
SYWP(Nle)Q
Probe #165
(FAM)-GSYWP(Nle)QGK(CPQ2)-PEG2-k-
842





NH2






166
ASQQHR
Probe #166
(FAM)-GASQQHRGK(CPQ2)-PEG2-k-NH2
843





167
KNPAKA
Probe #167
(FAM)-GKNPAKAGK(CPQ2)-PEG2-k-NH2
844





168
(Nle)YWLVE
Probe #168
(FAM)-G(Nle)YWLVEGK(CPQ2)-PEG2-k-
845





NH2






169
SWWIFE
Probe #169
(FAM)-GSWWIFEGK(CPQ2)-PEG2-k-NH2
846





170
VNYEQD
Probe #170
(FAM)-GVNYEQDGK(CPQ2)-PEG2-k-NH2
847





171
HFF(Nle)AE
Probe #171
(FAM)-GHFF(Nle)AEGK(CPQ2)-PEG2-kk-
848





NH2






172
DIPPHW
Probe #172
(FAM)-GDIPPHWGK(CPQ2)-PEG2-kk-NH2
849





173
VDQW(Nle)W
Probe #173
(FAM)-GVDQW(Nle)WGK(CPQ2)-PEG2-k-
850





NH2






174
LRSL(Nle)K
Probe #174
(FAM)-GLRSL(Nle)KGK(CPQ2)-PEG2-k-
851





NH2






175
(NleKNle)IRHA
Probe #175
(FAM)-G(Nle)(Nle)IRHAGK(CPQ2)-PEG2-k-
852





NH2






176
HDVKFI
Probe #176
(FAM)-GHDVKFIGK(CPQ2)-PEG2-kk-NH2
853





177
KRVQFL
Probe #177
(FAM)-GKRVQFLGK(CPQ2)-PEG2-k-NH2
854





178
RD(Nle)YAE
Probe #178
(FAM)-GRD(Nle)YAEGK(CPQ2)-NH2
855





179
L(Nle)IYFE
Probe #179
(FAM)-GL(Nle)IYFEGK(CPQ2)-PEG2-k-NH2
856





180
LRTKQS
Probe #180
(FAM)-GLRTKOSGK(CPQ2)-PEG2-k-NH2
857





181
WHGQQY
Probe #181
(FAM)-GWHGQQYGK(CPQ2)-PEG2-kk-
858





NH2






182
GPEGTI
Probe #182
(FAM)-GGPEGTIGK(CPQ2)-PEG2-k-NH2
859





183
ELDPIP
Probe #183
(FAM)-GELDPIPGK(CPQ2)-PEG2-k-NH2
860





184
GRAADF
Probe #184
(FAM)-GGRAADFGK(CPQ2)-NH2
861





185
HFIDYI
Probe #185
(FAM)-GHFIDYIGK(CPQ2)-PEG2-kk-NH2
862





186
S(Nle)(Nle)RVH
Probe #186
(FAM)-GS(Nle)(Nle)RVHGK(CPQ2)-PEG2-k-
863





NH2






187
SFRKII
Probe #187
(FAM)-GSFRKIIGK(CPQ2)-PEG2-k-NH2
864





188
TYE(Nle)FS
Probe #188
(FAM)-GTYE(Nle)FSGK(CPQ2)-PEG2-k-
865





NH2






189
HLLGFY
Probe #189
(FAM)-GHLLGFYGK(CPQ2)-PEG2-kk-NH2
866





190
(Nle)WTALT
Probe #190
(FAM)-G(Nle)WTALTGK(CPQ2)-PEG2-k-
867





NH2






191
IWN(Nle)VY
Probe #191
(FAM)-GIWN(Nle)VYGK(CPQ2)-PEG2-k-
868





NH2






192
RRNPLW
Probe #192
(FAM)-GRRNPLWGK(CPQ2)-PEG2-k-NH2
869





193
RWYGGI
Probe #193
(FAM)-GRWYGGIGK(CPQ2)-NH2
870





194
KTGDAR
Probe #194
(FAM)-GKTGDARGK(CPQ2)-PEG2-k-NH2
871





195
NYWEAN
Probe #195
(FAM)-GNYWEANGK(CPQ2)-PEG2-k-NH2
872





196
(Nle)QFDTS
Probe #196
(FAM)-G(Nle)QFDTSGK(CPQ2)-PEG2-k-
873





NH2






197
KRGAVE
Probe #197
(FAM)-GKRGAVEGK(CPQ2)-PEG2-k-NH2
874





198
SLKPTE
Probe #198
(FAM)-GSLKPTEGK(CPQ2)-NH2
875





199
ENDRLP
Probe #199
(FAM)-GENDRLPGK(CPQ2)-NH2
876





200
NSYQVQ
Probe #200
(FAM)-GNSYQVQGK(CPQ2)-PEG2-k-NH2
877





201
YPKEYL
Probe #201
(FAM)-GYPKEYLGK(CPQ2)-NH2
878





202
INNKWQ
Probe #202
(FAM)-GINNKWQGK(CPQ2)-NH2
879





203
(Nle)EFQGW
Probe #203
(FAM)-G(Nle)EFQGWGK(CPQ2)-PEG2-k-
880





NH2






204
PVRSTN
Probe #204
(FAM)-GPVRSTNGK(CPQ2)-NH2
881





205
sqaikv
Probe #205
(FAM)-GSQAIKVGK(CPQ2)-NH2
882





206
WA(NIe)LYH
Probe #206
(FAM)-GWA(Nle)LYHGK(CPQ2)-PEG2-kk-
883





NH2






207
ISWIHA
Probe #207
(FAM)-GISWIHAGK(CPQ2)-PEG2-kk-NH2
884





208
AHDIV
Probe #208
(FAM)-GAHDIVNGK(CPQ2)-PEG2-kk-NH2
885





209
RHNVAS
Probe #209
(FAM)-GRHNVASGK(CPQ2)-PEG2-k-NH2
886





210
SVFVIE
Probe #210
(FAM)-GSWVIEGK(CPQ2)-PEG2-k-NH2
887





211
FAKYYK
Probe #211
(FAM)-GFAKYYKGK(CPQ2)-PEG2-k-NH2
888





212
PYNTLQ
Probe #212
(FAMVGPYNTLOGK(CPQ2)-PEG2-k-NH2
889





213
(Nle)DWGH(Nle)
Probe #213
(FAM)-G(Nle)DWGH(Nle)GK(CPQ2)-PEG2-
890





kk-NH2






214
SNREWF
Probe #214
(FAM)-GSNREWFGK(CPQ2)-NH2
891





215
GKSEHT
Probe #215
(FAM)-GGKSEHTGK(CPQ2)-PEG2-kk-NH2
892





216
FP(Nle)TDQ
Probe #216
(FAM)-GFP(Nle)TDQGK(CPQ2)-PEG2-k-
893





NH2






217
WSKFW(Nle)
Probe #217
(FAM)-GWSKFW(Nle)GK(CPQ2)
894





218
RFTRPH
Probe #218
(FAM)-GRFTRPHGK(CPQ2)-NH2
895





219
QET(Nle)KD
Probe #219
(FAM)-GQET(Nle)KDGK(CPQ2)-NH2
896





220
HWWDVL
Probe #220
(FAM)-GHWWDVLGK(CPQ2)-PEG2-kk-
897





NH2






221
FNLV(Nle)S
Probe #221
(FAM)-GFNLV(Nle)SGK(CPQ2)-PEG2-k-
898





NH2






222
SAWRQR
Probe #222
(FAM)-GSAWRQRGK(CPQ2)-PEG2-k-NH2
899





223
TFHIFL
Probe #223
(FAM)-GTFHIFLGK(CPQ2)-PEG2-kk-NH2
900





224
WPQHVK
Probe #224
(FAM)-GWPQHVKGK(CPQ2)-PEG2-k-NH2
901





225
LI(Nle)HKN
Probe #225
(FAM)-GLI(Nle)HKNGK(CPQ2)-PEG2-k-
902





NH2






226
QDLEQP
Probe #226
(FAM)-GQDLEQPGK(CPQ2)-PEG2-k-NH2
903





227
HQKK(Nle)P
Probe #227
(FAM)-GHQKK(Nle)PGK(CPQ2)-NH2
904





228
GVTWLN
Probe #228
(FAM)-GGVTWLNGK(CPQ2)-PEG2-k-NH2
905





229
AGEPFK
Probe #229
(FAM)-GAGEPFKGK(CPQ2)-NH2
906





230
SR(Nle)ATT
Probe #230
(FAM)-GSR(Nle)ATTGK(CPQ2)-NH2
907





231
LAF(Nle)NH
Probe #231
(FAM)-GLAF(Nle)NHGK(CPQ2)-PEG2-kk-
908





NH2






232
PPSGLS
Probe #232
(FAM)-GPPSGLSGK(CPQ2)-PEG2-k-NH2
909





233
YTHSSP
Probe #233
(FAM)-GYTHSSPGK(CPQ2)-PEG2-kk-NH2
910





234
DGSHYR
Probe #234
(FAM)-GDGSHYRGK(CPQ2)-PEG2-kk-NH2
911





235
Y(Nle)GNGY
Probe #235
(FAM)-GY(Nle)GNGYGK(CPQ2)-PEG2-k-
912





NH2






236
DSITVS
Probe #236
(FAM)-GDSITVSGK(CPQ2)-PEG2-k-NH2
913





237
QTPNIQ
Probe #237
(FAM)-GQTPNIQGK(CPQ2)-PEG2-k-NH2
914





238
KLFFGY
Probe #238
(FAM)-GKLFFGYGK(CPQ2)-NH2
915





239
TQNFNW
Probe #239
(FAM)-GTQNFNWGK(CPQ2)-PEG2-k-NH2
916





240
YSDHEV
Probe #240
(FAM)-GYSDHEVGK(CPQ2)-PEG2-kk-NH2
917





241
RYVVPA
Probe #241
(FAM)-GRYVVPAGK(CPQ2)-NH2
918





242
ILHRIR
Probe #242
(FAM)-GILHRIRGK(CPQ2)-NH2
919





243
ESDNQ(Nle)
Probe #243
(FAM)-GESDNQ(Nle)GK(CPQ2)-PEG2-k-
920





NH2






244
YDDKG(Nle)
Probe #244
(FAM)-GYDDKG(Nle)GK(CPQ2)-NH2
921





245
QLS(Nle)VW
Probe #245
(FAM)-GQLS(Nle)VWGK(CPQ2)-PEG2-k-
922





NH2






246
PGGER(Nle)
Probe #246
(FAM)-GPGGER(Nle)GK(CPQ2)-NH2
923





247
WKHHPD
Probe #247
(FAM)-GWKHHPDGK(CPQ2)-NH2
924





248
QWVDED
Probe #248
(FAM)-GQWVDEDGK(CPQ2)-PEG2-k-NH2
925





249
NAYNEI
Probe #249
(FAM)-GNAYNEIGK(CPQ2)-PEG2-k-NH2
926





250
EEKAPR
Probe #250
(FAM)-GEEKAPRGK(CPQ2)-PEG2-kk-NH2
927





251
PWQIGK
Probe #251
(FAM)-GPWQIGKGK(CPQ2)-NH2
928





252
IAQVGN
Probe #252
(FAM)-GIAQVGNGK(CPQ2)-PEG2-k-NH2
929





253
V(Nle)RQSE
Probe #253
(FAM)-GV(Nle)RQSEGK(CPQ2)-NH2
930





254
TERVDA
Probe #254
(FAM)-GTERVDAGK(CPQ2)-NH2
931





255
WLRWRL
Probe #255
(FAM)-GWLRWRLGK(CPQ2)-PEG2-k-NH2
932





256
WKTKGQ
Probe #256
(FAM)-GWKTKGQGK(CPQ2)-PEG2-k-NH2
933





257
QSNGDV
Probe #257
(FAM)-GQSNGDVGK(CPQ2)-PEG2-k-NH2
934





258
TLFYAL
Probe #258
(FAM)-GTLFYALGK(CPQ2)-PEG2-k-NH2
935





259
TVTLNP
Probe #259
(FAM)-GTVTLNPGK(CPQ2)-PEG2-k-NH2
936





260
YAFGRK
Probe #260
(FAM)-GYAFGRKGK(CPQ2)-PEG2-k-NH2
937





261
DYNYWD
Probe #261
(FAM)-GDYNYWDGK(CPQ2)-PEG2-k-NH2
938





262
EWHEII
Probe #262
(FAM)-GEWHEIIGK(CPQ2)-PEG2-kk-NH2
939





263
QKAAWD
Probe #263
(FAM)-GQKAAWDGK(CPQ2)-NH2
940





264
DNTSAD
Probe #264
(FAM)-GDNTSADGK(CPQ2)-PEG2-k-NH2
941





265
HEGEYV
Probe #265
(FAM)-GHEGEYVGK(CPQ2)-PEG2-kk-NH2
942





266
WSPSFK
Probe #266
(FAM)-GWSPSFKGK(CPQ2)-NH2
943





267
HDEHWT
Probe #267
(FAM)-GHDEHWTGK(CPQ2)-PEG2-kk-NH2
944





268
YVW(Nle)RD
Probe #268
(FAM)-GYVW(Nle)RDGK(CPQ2)-NH2
945





269
(Nle)DP(Nle)KF
Probe #269
(FAM)-G(Nle)DP(Nle)KFGK(CPQ2)-NH2
946





270
(Nle)R(Nle)FW
Probe #270
(FAM)-G(Nle)R(Nle)FWDGK(CPQ2)-NH2
947



D








271
DIAIT(Nle)
Probe #271
(FAM)-GDIAIT(Nle)GK(CPQ2)-PEG2-k-NH2
948





272
PI(Nle)RFH
Probe #272
(FAM)-GPI(Nle)RFHGK(CPQ2)-PEG2-k-NH2
949





273
VWQGYI
Probe #273
(FAM)-GVWQGYIGK(CPQ2)-PEG2-k-NH2
950





274
KK(Nle)SNP
Probe #274
(FAM)-GKK(Nle)SNPGK(CPQ2)-PEG2-k-
951





NH2






275
GHPLSP
Probe #275
(FAM)-GGHPLSPGK(CPQ2)-PEG2-kk-NH2
952





276
VRQHKP
Probe #276
(FAM)-GVRQHKPGK(CPQ2)-NH2
953





277
AQNFYR
Probe #277
(FAM)-GAQNFYRGK(CPQ2)-NH2
954





278
VAGKSI
Probe #278
(FAM)-GVAGKSIGK(CPQ2)-NH2
955





279
LVGQVN
Probe #279
(FAM)-GLVGQVNGK(CPQ2)-PEG2-k-NH2
956





280
QVKHFT
Probe #280
(FAM)-GQVKHFTGK(CPQ2)-PEG2-k-NH2
957





281
QKSVVS
Probe #281
(FAM)-GQKSVVSGK(CPQ2)-NH2
958





282
Y(Nle)QEWL
Probe #282
(FAM)-GY(Nle)QEWLGK(CPQ2)-PEG2-k-
959





NH2






283
G(Nle)YIDE
Probe #283
(FAM)-GG(Nle)YIDEGK(CPQ2)-PEG2-k-
960





NH2






284
NAGSKF
Probe #284
(FAM)-GNAGSKFGK(CPQ2)-NH2
961





285
EFVHNP
Probe #285
(FAM)-GEFVHNPGK(CPQ2)-PEG2-kk-NH2
962





286
WE(Nle)VKI
Probe #286
(FAM)-GWE(Nle)VKIGK(CPQ2)-NH2
963





287
WVGASH
Probe #287
(FAM)-GWVGASHGK(CPQ2)-PEG2-kk-NH2
964





288
ITTLY(Nle)
Probe #288
(FAM)-GITTLY(Nle)GK(CPQ2)-PEG2-k-
965





NH2






289
GHIDEY
Probe #289
(FAM)-GGHIDEYGK(CPQ2)-PEG2-kk-NH2
966





290
KV(Nle)DYG
Probe #290
(FAM)-GKV(Nle)DYGGK(CPQ2)-NH2
967





291
QEKQT(Nle)
Probe #291
(FAM)-GQEKQT(Nle)GK(CPQ2)-NH2
968





292
EVGHEA
Probe #292
(FAM)-GEVGHEAGK(CPQ2)-PEG2-kk-NH2
969





293
AWEGQY
Probe #293
(FAM)-GAWEGQYGK(CPQ2)-PEG2-k-NH2
970





294
FLVQWT
Probe #294
(FAM)-GFLVQWTGK(CPQ2)-PEG2-k-NH2
971





295
SKWGYW
Probe #295
(FAM)-GSKWGYWGK(CPQ2)-NH2
972





296
TWIS(Nle)Q
Probe #296
(FAM)-GTWIS(Nle)QGK(CPQ2)-PEG2-k-
973





NH2






297
VIDKDF
Probe #297
(FAM)-GVIDKDFGK(CPQ2)-NH2
974





298
VKFAIY
Probe #298
(FAM)-GVKFAIYGK(CPQ2)-NH2
975





299
HNQ(Nle)KS
Probe #299
(FAM)-GHNQ(Nle)KSGK(CPQ2)-PEG2-k-
976





NH2






300
QYVFF(Nle)
Probe #300
(FAM)-GQYVFF(Nle)GK(CPQ2)-PEG2-k-
977





NH2






301
YNPRE(Nle)
Probe #301
(FAM)-GYNPRE(Nle)GK(CPQ2)-NH2
978





302
KHG(Nle)PE
Probe #302
(FAM)-GKHG(Nle)PEGK(CPQ2)-PEG2-kk-
979





NH2






303
WSREYW
Probe #303
(FAM)-GWSREYWGK(CPQ2)-NH2
980





304
IDRVDK
Probe #304
(FAM)-GIDRVDKGK(CPQ2)-
981





PEG2-kk-NH2






305
GDRENSPK(CP
Probe #305
(FAM)-kkGDRENSPK(CPQ2)L-OH
982



Q2)L-OH








306
GDRENSPLK(C
Probe #306
(FAM)-kkGDRENSPLK(CPQ2)-OH
983



PQ2)-OH








307
NAGSKFK(CPQ
Probe #307
(FAM)-GNAGSKFK(CPQ2)Q-OH
984



2)0-OH








308
NAGSKFQK(CP
Probe #308
(FAM)-GNAGSKFQK(CPQ2)-OH
985



Q2)-OH








309
GHLLGFYK(CP
Probe #309
(FAM)-kkGHLLGFYK(CPQ2)V-OH
986



Q2)V-OH








310
GHLLGFYVK(
Probe #310
(FAM)-kkGHLLGFYVK(CPQ2)-OH
987



CPQ2)-OH








311
GQEKQT(Nle)K
Probe #311
(FAM)-kkGQEKQT(Nle)K
988



(CPQ2)(Nle)-OH

(CPQ2)(Nle)-OH






312
GQEKQT(Nle)
Probe #312
(FAM)-kkGQEKQT(Nle)(Nle)
989



(Nle)K(CPQ2)-

K(CPQ2)-OH




OH








313
kGDPFVVSK(C
Probe #313
(FAM)-kGDPFVVSK(CPQ2)W-OH
990



P021W-OH








314
kGDPFVVSWK
Probe #314
(FAM)-kGDPFVVSWK(CPQ2)-OH
991



(CPQ2)-OH








315
NAYNE1K(CPQ
Probe #315
(FAM)-GNAYNEIK(CPQ2)R-OH
992



2)R-OH








316
NAYNEIRK(CP
Probe #316
(FAM)-GNAYNEIRK(CPQ2)-OH
993



Q2)-OH








317
V(Nle)RQSEK
Probe #317
(FAM)-GV(Nle)ROSEK(CPQ2)N-OH
994



(CPQ2)N-OH








318
V(Nle)RQSENK
Probe #318
(FAM)-GV(Nle)RQSENK(CPQ2)
995



(CPQ2)-OH








319
YNPRE(Nle)K
Probe #319
(FAM)-GYNPRE(Nle)K(CPQ2)I-OH
996



(CPQ2)I-OH








320
YNPRE(Nle)IK
Probe #320
(FAM)-GYNPRE(Nle)IK(CPQ2)-OH
997



(CPQ2)-OH








321
EFVHNPK(CPQ
Probe #321
(FAM)-kGEFVHNPK(CPQ2)K-OH
998



2)K-OH








322
EFVHNPKK(CP
Probe #322
(FAM)-kGEFVHNPKK(CPQ2)-OH
999



02VOH








323
KRVQFLK(CPQ
Probe #323
(FAM)-GKRVOFLK(CPQ2)H-OH
1000



2)H-OH








324
KRVQFLHK(CP
Probe #324
(FAM)-GKRVQFLHK(CPQ2)-OH
1001



Q2VOH








325
LI(Nle)HKNK(C
Probe #325
(FAM)-kGLI(Nle)HKNK(CPQ2)G-OH
1002



PQ2)G-OH








326
LI(Nle)HKNGK
Probe #326
(FAM)-kGLI(Nle)HKNGK(CPQ2)-OH
1003



(CPQ2)-OH








327
WA(Nle)LYHK
Probe #327
(FAM)-kkGWA(Nle)LYHK(CPQ2)S-OH
1004



(CPQ2)S-OH








328
WA(Nle)LYHS
Probe #328
(FAM)-kkGWA(Nle)LYHSK(CPQ2)-OH
1005



K(CPQ2)-OH








329
AHDIVNK(CPQ
Probe #329
(FAM)-kkGAHDIVNK(CPQ2)Y-OH
1006



2)Y-OH








330
AHDIVNYK(CP
Probe #330
(FAM)-kkGAHDIVNYK(CPQ2)-OH
1007



Q2)-OH








331
SVFVIEK(CPQ2
Probe #331
(FAM)-kGSVFVIEK(CPQ2)P-OH
1008



)P-OH








332
SVFVIEPK(CPQ
Probe #332
(FAM)-kGSVFVIEPK(CPQ2)-OH
1009



2)-OH








333
PPSGLSK(CPQ2)
Probe #333
(FAM)-kGPPSGLSK(CPQ2)E-OH
1010



E-OH








334
PPSGLSEK(CP
Probe #334
(FAM)-kGPPSGLSEK(CPQ2)-OH
1011



Q2)-OH








335
RWYGGIK(CP
Probe #335
(FAM)-kkGRWYGGIK(CPQ2)F-OH
1012



Q2)F-OH








336
RWYGGIFK(CP
Probe #336
(FAM)-kkGRWYGGIFK(CPQ2)-OH
1013



Q2)-OH








337
QYVFF(Nle)K(
Probe #337
(FAM)-kG0YVFF(Nle)K(CPQ2)D-OH
1014



CPQ2)D-OH








338
QYVFF(Nle)DK
Probe #338
(FAM)-kGOYVFF(Nle)DK(CPQ2)-OH
1015



(CPQ2)-OH








339
FAKYYKK(CP
Probe #339
(FAM)-kGFAKYYKK(CPQ2)T-OH
1016



Q2)T-OH








340
FAKYYKTK(CP
Probe #340
(FAM)-kGFAKYYKTK(CPQ2)-OH
1017



Q2)-OH








341
QVKHFTK(CPQ
Probe #341
(FAM)-kGQVKHFTK(CPQ2)A-OH
1018



2)A-OH








342
QVKHFTAK(CP
Probe #342
(FAM)-kGOVKHFTAK(CPQ2)-OH
1019



Q2)-OH








343
APK(CPQ2)-OH
Probe #343
FAM-APK(CPQ2)-OH
1020





344
NH2-
Probe #344
NH2-FK(FAM)DRENSPGK(CPQ2)-NH2
1021



HK(FAM)DREN






SP








345
NH2-
Probe #345
NH2-K(FAM)HDRENSPGK(CPQ2)-NH2
1022



K(FAM)HDREN






SP








346
NH2-
Probe #346
NH2-WK(FAM)NAGSKFGkK(CPQ2)-NH2
1023



WK(FAM)NAG






SKF








347
NH2-
Probe #347
NH2-K(FAM)WNAGSKFGkK(CPQ2)-NH2
1024



K(FAM)WNAG






SKF








348
NH2-
Probe #348
NH2-SK(FAM)HLLGFYGkK(CPQ2)-NH2
1025



SK(FAM)HLLG






FY








349
NH2-
Probe #349
NH2-K(FAM)SFILLGFYGkK(CPQ2)-NH2
1026



K(FAM)SHLLG






FY








350
NH2-
Probe #350
NH2-KK(FAM)QEKQT(Nle)GK(CPQ2)-NH2
1027



KK(FAM)QEKQ






T(Nle)








351
NH2-
Probe #351
NH2-K(FAM)KQEKQT(Nle)GK(CPQ2)-NH2
1028



K(FAM)KQEKQ






T(Nle)








352
NH2-
Probe #352
NH2-GK(FAM)DPFVVSGK(CPQ2)-NH2
1029



GK(FAM)DPFV






VS








353
NH2-
Probe #353
NH2-K(FAM)GDPFVVSGK(CPQ2)-NH2
1030



K(FAM)GDPFV






VS








354
NH2-
Probe #354
NH2-PK(FAM)NAYNEIGK(CPQ2)-NH2
1031



PK(FAM)NAYN






EI








355
NH2-
Probe #355
NH2-K(FAM)PNAYNEIGK(CPQ2)-NH2
1032



K(FAM)PNAYN






El








356
NH2-
Probe #356
NH2-DK(FAM)V(Nle)RQSEGkK(CPQ2)-
1033



DK(FAM)V

NH2




(Nle)RQSE








357
NH2-
Probe #357
NH2-K(FAM)DV(Nle)RQSEGkK(CPQ2)-
1034



K(FAM)DV(Nle)

NH2




RQSE








358
NH2-
Probe #358
NH2-EK(FAM)YNPRE(Nle)GkK(CPQ2)-NH2
1035



EK(FAM)YNPR






E(Nle)








359
NH2-
Probe #359
NH2-K(FAM)EYNPRE(Nle)GkK(CPQ2)-NH2
1036



K(FAM)EYNPR






E(Nle)








360
NH2-
Probe #360
NH2-TK(FAM)EFVHNPGkK(CPQ2)-NH2
1037



TK(FAM)EFVH






NP








361
NH2-
Probe #361
NH2-K(FAM)TEFVHNPGkK(CPQ2)-NH2
1038



K(FAM)TEFVH






NP








362
NH2-
Probe #362
NH2-QK(FAM)KRVQFLGK(CPQ2)-NH2
1039



QK(FAM)KRV






QFL








363
NH2-
Probe #363
NH2-K(FAM)QKRVQFLGK(CPQ2)-NH2
1040



K(FAM)QKRV






QFL








364
NH2-
Probe #364
NH2-YK(FAM)LI(Nle)HKNGK(CPQ2)-NH2
1041



YK(FAM)LI(Nle)






HKN








365
NH2-
Probe #365
NH2-K(FAM)YLI(Nle)HKNGK(CPQ2)-NH2
1042



K(FAM)YLI(Nle






)HKN








366
NH2-
Probe #366
NH2-FK(FAM)WA(Nle)LYHGkK(CPQ2)-
1043



FK(FAM)WA(N

NH2




le)LYH








367
NH2-
Probe #367
NH2-K(FAM)FWA(Nle)LYHGkK(CPQ2)-
1044



K(FAM)FWA(N

NH2




le)LYH








368
NH2-
Probe #368
NH2-IK(FAM)AHDIVNGkK(CPQ2)-NH2
1045



IK(FAM)AHDI






VN








369
NH2-
Probe #369
NH2-K(FAM)IAHDIVNGkK(CPQ2)-NH2
1046



K(FAM)IAHDI






VN








370
NH2-
Probe #370
NH2-VK(FAM)SVFVIEGK(CPQ2)-NH2
1047



VK(FAM)SVFV






IE








371
NH2-
Probe #371
NH2-K(FAM)VSVFVIEGK(CPQ2)-NH2
1048



K(FAM)VSVFV






IE








372
NH2-
Probe #372
NH2-(Nle)K(FAM)PPSGLSGK(CPQ2)-NH2
1049



(Nle)K(FAM)PP






SGLS








373
NH2-
Probe #373
NH2-K(FAM)(Nle)PPSGLSGK(CPQ2)-NH2
1050



K(FAM)(Nle)PP






SGLS








374
NH2-
Probe #374
NH2-LK(FAM)RWYGGIGkK(CPQ2)-NH2
1051



LK(FAM)RWY






GGI








375
NH2-
Probe #375
NH2-K(FAM)LRWYGGIGkK(CPQ2)-NH2
1052



K(FAM)LRWY






GGI








376
NH2-
Probe #376
NH2-NK(FAM)QYVFF(Nle)GK(CPQ2)-NH2
1053



NK(FAM)QYVF






F(Nle)








377
NH2-
Probe #377
NH2-K(FAM)NQYVFF(Nle)GK(CPQ2)-NH2
1054



K(FAM)NQYVF






F(Nle)








378
NH2-
Probe #378
NH2-AK(FAM)FAKYYKGK(CPQ2)-NH2
1055



AK(FAM)FAKY






YK








379
NH2-
Probe #379
NH2-K(FAM)AFAKYYKGK(CPQ2)-NH2
1056



K(FAM)AFAKY






YK








380
NH2-
Probe #380
NH2-RK(FAM)QVKHFTGK(CPQ2)-NH2
1057



RK(FAM)QVK






HFT








381
NH2-
Probe #381
NH2-K(FAM)RQVKHFTGK(CPQ2)-NH2
1058



K(FAM)RQVK






HFT








382
NH2-K(FAM)PP
Probe #382
NH2-K(FAM)PPK(CPQ2)-NH2
1059





383
kpilffrlk
Probe #383
5FAM-GkpilffrlkGK(CPQ2)-
1060





PEG2-kk-NH2






384
LRR
Probe #384
Boc-Leu-Arg-Arg-AMC
1061





385
R
Probe #385
Arg-AMC
1062





386
VR
Probe #386
Boc-Val-Arg-AMC
1063





387
RR
Probe #387
Z-Arg-Arg-AMC
1064





388
GR
Probe #388
Gly-Arg-AMC
1065





389
FR
Probe #389
Z-Phe-Arg-AMC
1066





390
RGK
Probe #390
Ac-Arg-Gly-Lys-AMC
1067





391
GGR
Probe #391
Z-Gly-Gly-Arg-AMC
1068





392
F
Probe #392
Glutaryl-Phe-AMC
1069





393
D
Probe #393
H-Asp-AMC
1070





394
RR
Probe #394
H-Arg-Arg-AMC
1071





395
R
Probe #395
Z-Arg-AMC
1072





396
Bz-R
Probe #396
Bz-Arg-AMC
1073





397
Bz-R
Probe #397
Bz-Arg-AMC
1073





398
PR
Probe #398
Z-Pro-Arg-AMC
1074





399
GPR
Probe #399
Z-Gly-Pro-Arg-AMC
1075





400
LR
Probe #400
Z-Leu-Arg-AMC
1076





401
PFR
Probe #401
H-Pro-Phe-Arg-AMC
1077





402
LLR
Probe #402
Z-Leu-Leu-Ary-AMC
1078





403
QRR
Probe #403
Boc-Gln-Arg-Arg-AMC
1079





404
GR
Probe #404
Glutaryl-Gly-Arg-AMC
1080





405
GRR
Probe #405
Boc-Gly-Arg-Arg-AMC
1081





406
LRGG
Probe #406
Z-Leu-Arg-Gly-Gly-AMC
1082





407
RLRGG
Probe #407
5-FAM-GRLRGGGK(CPQ2)-PEG2-kk-GC
1083





408
RELNGGAPI
Probe #408
5-FAM-GRELNGGAPIGK(CPQ2)-PEG2-kk-
1084





GC






409
TSAVLQSGFR
Probe #409
5-FAM-GTSAVLQSGFRKGK(CPQ2)-PEG2-
1085



K

kk-GC






410
SGVTFQGKFK
Probe #410
5-FAM-GSGVTFQGKFKKGK(CPQ2)-PEG2-
1086



K

kk-GC






411
AAFA
Probe #411
5-FAM-GAAFAGK(CPQ2)-PEG2-kk-GC
1087





412
HGDQMAQKS
Probe #412
5FAM-GHGDQMAQKS-K(CPQ2)-PEG2-
1088





DLys-DLys-GC-NH2






413
GPLGMR
Probe #413
5FAM-GGPLGMRG-K(CPQ2)-PEG2-DLys-
1089





DLys-GC-NH2






414
FFLAQA-
Probe #414
5FAM-GFFLAQA-HomoPhe-RSK-K(CPQ2)-
1090



HomoPhe-RSK

PEG2-DLys-DLys-GC-NH2






415
AHAVSRIRIYL
Probe #415
5FAM-GAHAVSRIRIYLLPAK-K(CPQ2)-
1091



LPAK

PEG2-DLys-DLys-GC-NH2






416
PLALWAR
Probe #416
5FAM-GPLALWAR-K(CPQ2)-PEG2-DLys-
1092





DLys-GC-NH2






417
PLA-
Probe #417
5FAM-GPLA-C(OMeBzl)-WAR-K(CPQ2)-
1093



C(OMeBzl)-

PEG2-DLys-DLys-GC-NH2




WAR








418
APRWIQD
Probe #418
5FAM-GAPRWIQD-K(CPQ2)-PEG2-DLys-
1094





DLys-GC-NH2






419
LREQQRLKS
Probe #419
5FAM-GLREQQRLKS-K(CPQ2)-PEG2-
1095





DLys-DLys-GC-NH2






420
EFPIYVFLPAK
Probe #420
5FAM-GEFPIYVFLPAKK-K(CPQ2)-PEG2-
1096



K

DLys-DLys-GC-NH2






421
GAANLVRGG
Probe #421
5FAM-GGAANLVRGG-K(CPQ2)-PEG2-
1097





DLys-DLys-GC-NH2






422
GYAELRMG
Probe #422
5FAM-GGYAELRMGG-K(CPQ2)-PEG2-
1098





DLys-DLys-GC-NH2






423
AAGAMFLEA
Probe #423
5FAM-GAAGAMFLEA-K(CPQ2)-PEG2-
1099





DLys-DLys-GC-NH2






424
LGGSGQRGRK
Probe #424
(FAM)-GLGGSGQRGRKALEG-K(CPQ2)-
1100



ALE

(PEG2)-DLys-DLys-GC






425
LGGSGHYGRS
Probe #425
(FAM)-GLGGSGHYGRSGLEG-K(CPQ2)-
1101



GLE

(PEG2)-DLys-DLys-GC






426
YGRS
Probe #426
(FAM)-GYGRSG-K(CPQ2)-(PEG2)-DLys-
1102





DLys-GC






427
FRGRK
Probe #427
(FAM)-GFRGRKG-K(CPQ2)-(PEG2)-DLys-
1103





DLvs-GC






428
DRRKKLTQ
Probe #428
(FAM)-GDRRKKLTQG-K(CPQ2)-(PEG2)-
1104





DLys-DLys-GC






429
HPGGPQ
Probe #429
(FAM)-GHPGGPQG-K(CPQ2)-(PEG2)-DLys-
1105





DLys-GC






430
KLRFSKQ
Probe #430
(FAM)-GKLRFSKQG-K(CPQ2)-(PEG2)-
1106





DLys-DLys-GC






431
AIKFFSAQ
Probe #431
(FAM)-GAIKFFSAQG-K(CPQ2)-(PEG2)-
1107





DLys-DLys-GC






432
AIKFFVRQ
Probe #432
(FAM)-GAIKFFVRQG-K(CPQ2)-(PEG2)-
1108





DLys-DLys-GC






433
RPPGFSAFK
Probe #433
(FAM)-GRPPGFSAFKG-K(CPQ2)-(PEG2)-
1109





DLys-DLys-GC






434
FAP-QLS
Probe #434
(FAM)-GFAP-QLSG-K(CPQ2)-(PEG2)-DLys-
1110





DLys-GC






435
FAA-QMA
Probe #435
(FAM)-GFAA-QMAG-K(CPQ2)-(PEG2)-
1111





DLys-DLys-GC






436
GMP-ANQ
Probe #436
(FAM)-GGMP-ANQG-K(CPQ2)-(PEG2)-
1112





DLys-DLys-GC






437
LSGRSDNH
Probe #437
(FAM)-GLSGRSDNHG-K(CPQ2)-(PEG2)-
1113





DLys-DLys-GC






438
MAALITRPDF
Probe #438
(FAM)-GMAALITRPDFG-K(CPQ2)-(PEG2)-
1114





DLys-DLys-GC






439
MAAAITRPRF
Probe #439
(FAM)-GMAAAITRPRFG-K(CPQ2)-(PEG2)-
1115





DLys-DLys-GC






440
MAALIVRPDL
Probe #440
(FAM)-GMAALIVRPDLG-K(CPQ2)-(PEG2)-
1116





DLys-DLys-GC






441
TSGPNQEQE
Probe #441
(FAM)-GTSGPNQEQEG-K(CPQ2)-(PEG2)-
1117





DLys-DLys-GC






442
TAGPNQEQE
Probe #442
(FAM)-GTAGPNQEQEG-K(CPQ2)-(PEG2)-
1118





DLys-DLys-GC






443
GPGPNQA
Probe #443
(FAM)-GGPGPNQAG-K(CPQ2)-(PEG2)-
1119





DLys-DLys-GC






444
ASGPAGPA
Probe #444
(FAM)-GASGPAGPAG-K(CPQ2)-(PEG2)-
1120





DLys-DLys-GC






445
ERGETGPSG
Probe #445
(FAM)-GERGETGPSGG-K(CPQ2)-(PEG2)-
1121





DLys-DLys-GC






446
VSQELGQR
Probe #446
(FAM)-GVSQELGQRG-K(CPQ2)-(PEG2)-
1122





DLys-DLys-GC






447
TGPPGYPTG
Probe #447
(FAM)-GTGPPGYPTGG-K(CPQ2)-(PEG2)-
1123





DLys-DLys-GC






448
TRLPVYQ
Probe #448
(FAM)-GTRLPVYQG-K(CPQ2)-(PEG2)-
1124





DLys-DLys-GC






449
RQARVVGG
Probe #449
(FAM)-GRQARWGGG-K(CPQ2)-(PEG2)-
1125





DLys-DLys-GC






450
RQRRVVGG
Probe #450
(FAM)-GRQRRVVGGG-K(CPQ2)-(PEG2)-
1126





DLys-DLys-GC






451
RQARAVGG
Probe #451
(FAM)-GRQARAVGGG-K(CPQ2)-(PEG2)-
1127





DLys-DLys-GC






452
RKRRGSRG
Probe #452
(FAM)-GRKRRGSRGG-K(CPQ2)-(PEG2)-
1128





DLys-DLys-GC






453
KQSRKFVP
Probe #453
(FAM)-GKQSRKFVPG-K(CPQ2)-(PEG2)-
1129





DLys-DLys-GC






454
VTGRS
Probe #454
(FAM)-GVTGRSG-K(CPQ2)-(PEG2)-DLys-
1130





DLys-GC






455
LKSRVK
Probe #455
(FAM)-GLKSRVKG-K(CPQ2)-(PEG2)-DLys-
1131





DLys-GC






456
GIGAVLKVLT
Probe #456
(FAM)-GGIGAVLKVLTG-K(CPQ2)-(PEG2)-
1132





DLys-DLys-GC






457
GLPALISWIK
Probe #457
(FAM)-GGLPALISWIKG-K(CPQ2)-(PEG2)-
1133





DLys-DLys-GC






458
SEVNLDAEF
Probe #458
(FAM)-GSEVNLDAEFG-K(CPQ2)-(PEG2)-
1134





DLys-DLys-GC






459
EEKPICFFRLG
Probe #459
(FAM)-GEEKPICFFRLGKEG-K(CPQ2)-
1135



KE

(PEG2)-DLys-DLys-GC






460
EEKPILFFRLG
Probe #460
(FAM)-GEEKPILFFRLGKEG-K(CPQ2)-
1136



KE

(PEG2)-DLys-DLys-GC






461
APSSVIAA
Probe #461
(FAM)-GAPSSVIAAG-K(CPQ2)-(PEG2)-
1137





DLys-DLys-GC






462
KKAKRNAL
Probe #462
(FAM)-GKKAKRNALG-K(CPQ2)-(PEG2)-
1138





DLys-DLys-GC






463
WTNTSANYNL
Probe #463
(FAM)-GWTNTSANYNLG-K(CPQ2)-
1139





(PEG2)-DLys-DLys-GC






464
RVRR
Probe #464
(FAM)-GRVRRG-K(CPQ2)-(PEG2)-DLys-
1140





DLys-GC






465
ERTKR
Probe #465
(FAM)-GERTKRG-K(CPQ2)-(PEG2)-DLys-
1141





DLys-GC






466
RYQIKPLKSTD
Probe #466
(FAM)-GRYQIKPLKSTDEG-K(CPQ2)-
1142



E

(PEG2)-DLys-DLys-GC






467
WELRHQA-
Probe #467
(FAM)-GWELRHQA-(Hfe)-RSKG-K(CPQ2)-
1143



(Hfe)-RSK

(PEG2)-DLys-DLys-GC






468
SGAFK-C(Me)-
Probe #468
(FAM)-GSGAFK-C(Me)-LKDGAGG-
1144



LKDGAG

K(CPQ2)-(PEG2)-DLys-DLys-GC






469
YVADGW
Probe #469
(FAM)-GYVADGWG-K(CPQ2)-(PEG2)-
1145





DLys-DLys-GC






470
WEHDGW
Probe #470
(FAM)-GWEHDGWG-K(CPQ2)-(PEG2)-
1146





DLys-DLys-GC






471
YVADAPV
Probe #471
(FAM)-GYVADAPVG-K(CPQ2)-(PEG2)-
1147





DLys-DLys-GC






472
RPPGFSA
Probe #472
(FAM)-GRPPGFSAG-K(CPQ2)-(PEG2)-
1148





DLys-DLys-GC






473
GSPAFLA
Probe #473
(FAM)-GGSPAFLAG-K(CPQ2)-(PEG2)-
1149





DLys-DLys-GC






474
AGFSLPA
Probe #474
(FAM)-GAGFSLPAG-K(CPQ2)-(PEG2)-
1150





DLys-DLys-GC






475
RWHTVGLRW
Probe #475
(FAM)-GRWHTVGLRWEG-K(CPQ2)-
1151



E

(PEG2)-DLys-DLys-GC






476
LEQ
Probe #476
(FAM)-GLEQG-K(CPQ2)-(PEG2)-DLys-
1152





DLys-GC






477
RWPPMGLPWE
Probe #477
(FAM)-GRWPPMGLPWEG-K(CPQ2)-
1153





(PEG2)-DLys-DLys-GC






478
RPKPVE
Probe #478
(FAM)-GRPKPVEG-K(CPQ2)-(PEG2)-DLys-
1154





DLys-GC






479
IETD
Probe #479
(FAM)-GIETDG-K(CPQ2)-(PEG2)-DLys-
1155





DLys-GC






480
VGPDFGR
Probe #480
(FAM)-GVGPDFGRG-K(CPQ2)-(PEG2)-
1156





DLys-DLys-GC






481
GIEFDSGGC
Probe #481
(FAM)-GGIEFDSGGCG-K(CPQ2)-(PEG2)-
1157





DLys-DLys-GC






482
GDFLRRV
Probe #482
(FAM)-GGDFLRRVG-K(CPQ2)-(PEG2)-
1158





DLys-DLys-GC






483
AAL
Probe #483
(FAM)-GAALG-K(CPQ2)-(PEG2)-DLys-
1159





DLys-GC






484
YATWSMIAAH
Probe #484
(FAM)-GYATWSMIAAHG-K(CPQ2)-
1160





(PEG2)-DLys-DLys-GC






485
VIMWRLTVGT
Probe #485
(FAM)-GVIMWRLTVGTG-K(CPQ2)-
1161





(PEG2)-DLys-DLys-GC






486
RRVLALQQEL
Probe #486
(FAM)-GRRVLALQQELG-K(CPQ2)-(PEG2)-
1162





DLys-DLys-GC






487
LATWPLSGLW
Probe #487
(FAM)-GLATWPLSGLWG-K(CPQ2)-
1163





(PEG2)-DLys-DLys-GC






488
NTPNWLVNAV
Probe #488
(FAM)-GNTPNWLVNAVG-K(CPQ2)-
1164





(PEG2)-DLys-DLys-GC






489
SPLAQAVRSSS
Probe #489
(FAM)-GSPLAQAVRSSSRKG-K(CPQ2)-
1165



RK

(PEG2)-DLys-DLys-GC






490
QMPGRLSMAF
Probe #490
(FAM)-GQMPGRLSMAFG-K(CPQ2)-
1166





(PEG2)-DLys-DLys-GC






491
PLGLR
Probe #491
(FAM)-GPLGLRG-K(CPQ2)-(PEG2)-DLys-
1167





DLys-GC






492
QRANSIRVTW
Probe #492
(FAM)-GQRANSIRVTWG-K(CPQ2)-(PEG2)-
1168





DLys-DLys-GC






493
PLAVR
Probe #493
(FAM)-GPLAVRG-K(CPQ2)-(PEG2)-DLys-
1169





DLys-GC






494
LLAVPAANTV
Probe #494
(FAM)-GLLAVPAANTVG-K(CPQ2)-
1170





(PEG2)-DLys-DLys-GC






495
GPQGLRGQ
Probe #495
(FAM)-GGPQGLRGQG-K(CPQ2)-(PEG2)-
1171





DLys-DLys-GC






496
RTGLYLYNST
Probe #496
(FAM)-GRTGLYLYNSTG-K(CPQ2)-(PEG2)-
1172





DLys-DLys-GC






497
RKKLTQSKFV
Probe #497
(FAM)-GRKKLTQSKFVGGAEG-K(CPQ2)-
1173



GGAE

(PEG2)-DLys-DLys-GC






498
KHYR
Probe #498
(FAM)-GKHYRG-K(CPQ2)-(PEG2)-DLys-
1174





DLys-GC






499
QAR
Probe #499
(FAM)-GQARG-K(CPQ2)-(PEG2)-DLys-
1175





DLys-GC






500
PRPFNYL
Probe #500
(FAM)-GPRPFNYLG-K(CPQ2)-(PEG2)-
1176





DLys-GC






501
APFEMSA
Probe #501
(FAM)-GAPFEMSAG-K(CPQ2)-(PEG2)-
1177





DLys-DLys-GC






502
APFEFSA
Probe #502
(FAM)-GAPFEFSAG-K(CPQ2)-(PEG2)-
1178





DLys-DLys-GC






503
PLGFRV
Probe #503
(FAM)-GPLGFRVG-K(CPQ2)-(PEG2)-DLys-
1179





GC






504
RPLALWRS
Probe #504
(FAM)-GRPLALWRSG-K(CPQ2)-(PEG2)-GC
1180





505
RPLALEESQ
Probe #505
(FAM)-GRPLALWRSG-K(CPQ2)-(PEG2)-GC
1181





DLys-GC






506
RPLALWRSQ
Probe #506
(FAM)-GRPLALWRSQG-K(CPQ2)-(PEG2)-
1182





GC






507
RNALAVERTA
Probe #507
(FAM)-GRNALAVERTASG-K(CPQ2)-
1183



S

(PEG2)-GC






508
RPKPQQFW
Probe #508
(FAM)-GRPKPQQFWG-K(CPQ2)-(PEG2)-
1184





DLys-GC






509
SGSNPYKYTA
Probe #509
(FAM)-SGSNPYKYTA-K(CPQ2)-(PEG2)-
1185





DLys-DLys-GC






510
SGSNPYGYTA
Probe #510
(FAM)-SGSNPYGYTA-K(CPQ2)-(PEG2)-
1186





DLys-DLys-GC






511
SGTLSELHTA
Probe #511
(FAM)-SGTLSELHTA-K(CPQ2)-(PEG2)-
1187





DLys-DLys-GC






512
SGTISHLHTA
Probe #512
(FAM)-SGTISHLHTA-K(CPQ2)-(PEG2)-
1188





DLys-DLys-GC






513
SG-(Orn)-RSHP-
Probe #513
(FAM)-SG-(Orn)-RSHP-(Hfe)-TLYTA-
1189



(Hfe)-TLYTA

K(CPQ2)-(PEG2)-DLys-GC






514
SG-(Orn)-
Probe #514
(FAM)-SG-(Orn)-RSHG-(Hfe)-FLYTA-
1190



RSHG-(Hfe)-

K(CPQ2)-(PEG2)-DLys-GC




FLYTA








515
SGESLAYYTA
Probe #515
(FAM)-SGESLAYYTA-K(CPQ2)-(PEG2)-
1191





DLys-DLys-GC






516
SGHMH AALT A
Probe #516
(FAM)-SGHMHAALTA-K(CPQ2)-(PEG2)-
1192





DLys-DLys-GC






517
ILSR-(DIle)-
Probe #517
(FAM)-GILSR-(Dlle)-VGGG-K(CPQ2)-
1193



VGG

(PEG2)-DLys-GC






518
ILS-(DArg)-
Probe #518
(FAM)-GILS-(DArg)-(DIle)-(DVal)-GGG-
1194



(DIle)-(DVal)-

K(CPQ2)-(PEG2)-DLys-GC




GG








519
RQRRALEK
Probe #519
5FAM-GRQRRALEKG-K(CPQ2)-PEG2-GC
1195





520
KPISLISS
Probe #520
5FAM-GKPISLISSG-K(CPQ2)-PEG2-GC
1196





521
QKGRYKQE
Probe #521
5FAM-GQKGRYKQEG-K(CPQ2)-PEG2-GC
1197





522
GPLGLRSW
Probe #522
5FAM-GGPLGLRSWK(CPQ2)-PEG2-C
1198





523
GPLGVRGK
Probe #523
5FAM-GGPLGVRGKK(CPQ2)-PEG2-C
1199





524
GfPRSGG
Probe #524
5FAM-GGfPRSGGGK(CPQ2)-PEG2-C
1200





525
Pyr
Probe #525
Pyr-AMC
1201





526
SY
Probe #526
H-Ser-Tyr-AMC
1202





527
GF
Probe #527
H-Gly-Phe-AMC
1203





528
Y
Probe #528
H-Tyr-AMC
1204





529
Cit
Probe #529
H-Cit-AMC Hydrobromide salt
1205





530
GP
Probe #530
Suc-Gly-Pro-AMC
1206





531
T
Probe #531
H-Thr-AMC
1207





532
I
Probe #532
H-Ile-AMC
1208





533
GA
Probe #533
H-Gly-Ala-AMC hydrochloride salt
1209





534
Cys(Bzl)
Probe #534
H-Cys(Bzl)-AMC
1210





535
A
Probe #535
H-Ala-AMC
1211





536
K
Probe #536
Ac-Lys-AMC acetate salt
1212





537
GLF
Probe #537
MeOSuc-Gly-Leu-Phe-AMC
1213





538
L
Probe #538
H-Leu-AMC
1214





539
VAN
Probe #539
Z-Val-Ala-Asn-AMC
1215





540
AAA
Probe #540
Suc-Ala-Ala-Ala-AMC
1216





541
K
Probe #541
H-Lys-AMC acetate salt
1217





542
F
Probe #542
H-Phe-AMC trifluoroacetate salt
1218





543
FSR
Probe #543
Boc-Phe-Ser-Arg-AMC
1219





544
VVR
Probe #544
Z-Val-Val-Arg-AMC
1220





hydrochloride salt






545
KA
Probe #545
H-Lys-Ala-AMC
1221





hydrochloride salt






546
PR
Probe #546
H-Pro-Arg-AMC
1222





hydrochloride salt






547
MGP
Probe #547
H-Met-Gly-Pro-AMC
1223





hydrochloride salt






548
KP
Probe #548
H-Lys-Pro-AMC
1224





hydrochloride salt






549
QGR
Probe #549
Boc-Gln-Gly-Arg-AMC
1225





hydrochloride salt






550
Glu(OBzl)-AR
Probe #550
Boc-Glu(OBzl)-Ala-Arg-AMC
1226





hydrochloride






salt






551
WEHD
Probe #551
Ac-Trp-Glu-His-Asp-AMC
1227





552
QAR
Probe #552
Boc-Gln-Ala-Arg-AMC
1228





hydrochloride salt






553
AAF
Probe #553
H-Ala-Ala-Phe-AMC (free base)
1229





554
GPK
Probe #554
Tos-Gly-Pro-Lys-AMC
1230





trifluoroacetate salt






555
AAPM
Probe #555
MeOSuc-Ala-Ala-Pro-Met-AMC
1231





556
AEPF
Probe #556
Suc-Ala-Glu-Pro-Phe-AMC
1232





557
GG
Probe #557
H-Gly-Gly-AMC
1233





hydrochloride salt






558
VLK
Probe #558
Boc-Val-Leu-Lys-AMC
1234





acetate salt






559
EKK
Probe #559
Boc-Glu-Lys-Lys-AMC
1235





acetate salt






560
VPR
Probe #560
Boc-Val-Pro-Arg-AMC
1236





hydrochloride salt






561
GKR
Probe #561
Boc-Gly-Lys-Arg-AMC
1237





hydrochloride salt






562
Glu(OBzl)-GR
Probe #562
Boc-Glu(OBzl)-Gly-Arg-AMC
1238





hydrochloride






salt






563
LR
Probe #563
Z-Leu-Arg-AMC
1239





hydrochloride salt






564
AFK
Probe #564
MeOSuc-Ala-Phe-Lys-AMC
1240





trifluoroacetate






salt






565
LGR
Probe #565
Boc-Leu-Gly-Arg-AMC
1241





acetate salt






566
PFR
Probe #566
H-Pro-Phe-Arg-AMC
1242





acetate salt






567
AAPV
Probe #567
Suc-Ala-Ala-Pro-Val-AMC
1243





568
AFK
Probe #568
H-Ala-Phe-Lys-AMC
1244





trifluoroacetate salt






569
VKM
Probe #569
Z-Val-Lys-Met-AMC acetate salt
1245





570
GPLGP
Probe #570
Suc-Gly-Pro-Leu-Gly-Pro-AMC
1246





571
KQKER
Probe #571
Ac-Lys-Gln-Lys-Leu-Arg-AMC
1247





trifluoroacetate salt






572
RVRR
Probe #572
Boc-Arg-Val-Arg-Arg-AMC
1248





acetate salt






573
IEGR
Probe #573
Boc-Ile-Glu-Gly-Arg-AMC
1249





acetate salt






574
GP
Probe #574
H-Gly-Pro-AMC HBr
1250





575
AAPV
Probe #575
MeOSuc-Ala-Ala-Pro-Val-AMC
1251





576
RPFHLLVY
Probe #576
Suc-Arg-Pro-Phe-His-Leu-
1252





Leu-Val-Tyr-AMC






trifluoroacetate salt






577
Anb-WS-Gnf-
Probe #577
H-Anb-Trp-Ser-Gnf-Thr-Val-Phe-AMC
1253



TVF








578
HSSKLQ
Probe #578
Mu-His-Ser-Ser-Lys-Leu-Gln-AMC
1254





579
RPY
Probe #579
MeO-Succ-Arg-Pro-Tyr-AMC
1255





580
DRENSPK(Dnp)
Probe #580
(ACO-kkDRENSPK(Dnp)L
1256



L-OH








581
kkDRENSPLK
Probe #581
(ACC)-kkDRENSPLK(Dnp)
1257



(Dnp)-OH








582
NAGSKFK(Dnp)
Probe #582
(ACC)-NAGSKFK(Dnp)Q
1258



Q-OH








583
NAGSKFQK(Dn
Probe #583
(ACC)-NAGSKFQK(Dnp)
1259



p)-OH








584
HLLGFYK(Dnp)
Probe #584
(ACC)-kkHLLGFYK(Dnp)V
1260



V-OH








585
HLLGFYVK(Dn
Probe #585
(ACC)-kkHLLGFYVK(Dnp)
1261



p)-OH








586
QEKQT(Nle)K(
Probe #586
(ACC)-kkQEKQT(Nie)K(Dnp)(Nle)
1262



Dnp)(Nle)-OH








587
QEKQT(Nle)(Nl
Probe #587
(ACC)-kkQEKQT(Nle)(Nle)K(Dnp)
1263



e)K(Dnp)-OH








588
DPFVVSK(Dnp)
Probe #588
(ACC)-kDPFVVSK(Dnp)W
1264



W-OH








589
DPFVVSWK(Dn
Probe #589
(ACC)-kDPFVVSWK(Dnp)
1265



p)-OH








590
NAYNEIK(Dnp)
Probe #590
(ACC)-NAYNEIK(Dnp)R
1266



R-OH








591
NAYNEIRK(Dn
Probe #591
(ACC)-NAYNEIRK(Dnp)
1267



p)-OH








592
V(Nle)RQSEK(
Probe #592
(ACC)-V(Nle)RQSEK(Dnp)N
1268



Dnp)N-OH








593
V(Nle)RQSENK
Probe #593
(ACC)-V(Nle)RQSENK(Dnp)
1269



(Dnp)-OH








594
YNPRE(Nle)K
Probe #594
(ACC)-YNPRE(Nle)K(Dnp)I
1270



(Dnp)I-OH








595
YNPRE(Nle)IK
Probe #595
(ACC)-YNPRE(Nle)IK(Dnp)
1271



(Dnp)-OH








596
EFVHNPK(Dnp)
Probe #596
(ACC)-kEFVHNPK(Dnp)K
1272



K-OH








597
EFVHNPKK
Probe #597
(ACC)-kEFVHNPKK(Dnp)
1273



(Dnp)-OH








598
KRVQFLK
Probe #598
(ACC)-KRVQFLK(Dnp)H
1274



(Dnp)H-OH








599
KRVQFLHK
Probe #599
(ACC)-KRVQFLHK(Dnp)
1275



(Dnp)-OH








600
LI(Nle)HKNK
Probe #600
(ACC)-kLI(Nle)HKNK(Dnp)G
1276



(Dnp)G-OH








601
LI(Nle)HKNGK
Probe #601
(ACC)-kLI(Nle)HKNGK(Dnp)
1277



(Dnp)-OH








602(
WA(Nle)LYHK
Probe #602
(ACC)-kkWA(Nle)LYFIK(Dnp)S
1278



Dnp)S-OH








603
WA(Nle)LYHS
Probe #603
(ACC)-kkWA(Nle)LYHSK(Dnp)
1279



K(Dnp)-OH








604
AHDIVNK(Dnp)
Probe #604
(ACC)-kkAHDIVNK(Dnp)Y
1280



Y-OH








605
AHDIVNYK
Probe #605
(ACC)-kkAHDIVNYK(Dnp)
1281



(Dnp)-OH








606
SVFVIEK(Dnp)
Probe #606
(ACC)-kSVFVIEK(Dnp)P
1282



P-OH








607
SVFVIEPK
Probe #607
(ACC)-kSVFVIEPK(Dnp)
1283



(Dnp)-OH








608
PPSGLSK(Dnp)
Probe #608
(ACC)-kPPSGLSK(Dnp)E
1284



E-OH








609
PPSGLSEK
Probe #609
(ACC)-kPPSGLSEK(Dnp)
1285



(Dnp)-OH








610
RWYGGIK
Probe #610
(ACC)-kkRWYGGIK(Dnp)F
1286



(Dnp)F-OH








611
RWYGGIFK(Dn
Probe #611
(ACC)-kkRWYGGIFK(Dnp)
1287



p)-OH








612
QYVFF(Nle)K
Probe #612
(ACC)-kQYVFF(Nle)K(Dnp)D
1288



(Dnp)D-OH








613
QYVFF(Nle)DK
Probe #613
(ACC)-kQYVFF(Nle)DK(Dnp)
1289



(Dnp)-OH








614
FAKYYKK
Probe #614
(ACC)-kFAKYYKK(Dnp)T
1290



(Dnp)T-OH








615
FAKYYKTK
Probe #615
(ACC)-kFAKYYKTK(Dnp)
1291



(Dnp)-OH








616
QVKHFTK
Probe #616
(ACC)-kQVKHFTK(Dnp)A
1292



(Dnp)A-OH








617
QVKHFTAK
Probe #617
(ACC)-kQVKHFTAK(Dnp)
1293



(Dnp)-OH








618
YVADAPK
Probe #618
(ACC)-kYVADAPK(Dnp)
1294



(Dnp)-OH








619
KGISSQY
Probe #619
ACC-GKGISSQYK(Dnp)-NH2
1295





620
ALPALQN
Probe #620
ACC-GALPALQNK(Dnp)-PEG2-Dlys-Dlys-
1296





NH2






621
HRFRG
Probe #621
ACC-GHRFRGK(Dnp)-NH2
1297





622
APEEIMDQQ
Probe #622
ACC-GAPEEIMDQQK(Dnp)-PEG2-Dlys-
1298





Dlys-NH2






623
SRKSQQY
Probe #623
ACC-GSRKSQQYK(Dnp)-NH2
1299





624
SKGRSLI
Probe #624
ACC-GSKGRSLIGK(Dnp)-NH2
1300





625
FAQSIPK
Probe #625
ACC-GFAQSIPKK(Dnp)-PEG2-Dlys-Dlys-
1301





NH2






626
RQRRVVG
Probe #626
ACC-GRQRRVVGGK(Dnp)-NH2
1302





627
ERGETGPS
Probe #627
ACC-GERGETGPSGK(Dnp)-NH2
1303





628
ASGPSS
Probe #628
ACC-GASGPSSGK(Dnp)-PEG2-Dlys-Dlys-
1304





NH2






629
YRFR
Probe #629
ACC-GYRFRGK(Dnp)-NH2
1305





630
KLFSSKQ
Probe #630
ACC-GKLFSSKQK(Dnp)-NH2
1306





631
IVPRG
Probe #631
ACC-GIVPRGK(Dnp)-NH2
1307





632
IRRSSYFK
Probe #632
ACC-GIRRSSYFKK(Dnp)-NH2
1308





633
His(Bzl)-Tle-
Probe #633
ACC-Gly-His(Bzl)-Tle-Pro-Ser-Asp-Met(O)-
1309



PSD-Met(O)

Gly-K(Dnp)-Gly-PEG2-Dlys-Dlys-NH2






634
Nva-IE-Oic-
Probe #634
ACC-Nva-Ile-Glu-Oic-Asp-Phe-Gly-Arg-
1310



DFGR

LVs(Dnp)-NH2






635
H-DThr-
Probe #635
Ac-His-DThr-Phe(F5)-Arg-ACC
1311



Phe(F5)-R








636
Dap-Om-
Probe #636
Ac-Dap-Orn-Phe(3Cl)-Cys(MeOBzl)-ACC
1312



Phe(3Cl)-






Cys(MeOBzl)








637
Cha-L-
Probe #637
Ac-Cha-Leu-hSer(Bzl)-Arg-ACC
1313



hSer(Bzl)-R








638
His(Bzl)-Tle-
Probe #638
ACC-Gly-His(Bzl)-Tle-Pro-Ser-Asp-Met(O)-
1309



PSD-Met(O)

Gly-K(Dnp)-Gly-PEG2-Dlys-Dlys-NH2






639
hCha-Phe(guan)-
Probe #639
Ac-hCha-Phe(guan)-Oic-Arg-ACC
1314



Oic-R








640
Abu-Nle(O-Bzl)
Probe #640
NH2-Abu-Nle(O-Bzl)-ACC
1315





641
Nle(O-Bzl)-
Probe #641
Ac-Nle(O-Bzl)-Met(O)2-Oic-Abu-ACC
1316



Met(O)2-Oic-






Abu








642
Dap-Om-
Probe #642
ACC-G-Dap-Om-Phe(3Cl)-Cys(MeOBz)-G-
1317



Phe(3Cl)-

K(Dnp)-NH2




Cvs(MeOBz)








643
Cha-L-hSer-R
Probe #643
ACC-Gly-Cha-Leu-hSer-Arg-Gly-K(Dnp)-
1318





NH2






644
FVT-Gnf-SW
Probe #644
ACC-Phe-Val-Thr-Gnf-Ser-Trp-K(Dnp)-NH2
1319





645
hCha-Phe(guan)-
Probe #645
ACC-Gly-hCha-Phe(guan)-Oic-Arg-Gly-
1320



Oic-R

K(Dnp)-NH2






646
Nle(OBz)-
Probe #646
ACC-Gly-Nle(OBz)-Met(O2)-Oic-Abu-Gly-
1321



Met(O2)-Oic-

K(Dnp)-NH2




Abu








647
AIEPDSG
Probe #647
5FAM-GAIEPDSGG-Lys(CPQ2)-PEG2-Dlys-
1322





Dlys-GC-NH2






648
A1EFDSG
Probe #648
5FAM-GAIEFDSGG-Lys(CPQ2)-Dlys-Dlys-
1323





GC-NH2






649
AAEAISD
Probe #649
5FAM-GGAAEAISDAK(CPQ2)-kk-PEG2-C
1324





650
AGGAQMGA
Probe #650
5FAM-GGAGGAQMGAK(CPQ2)-kk-PEG2-
1325





C






651
AQPDALNV
Probe #651
5FAM-GGAQPDALNVK(CPQ2)-kk-PEG2-C
1326





652
ATDVTTTP
Probe #652
5FAM-GGATDVTTTPK(CPQ2)-kk-PEG2-C
1327





653
DIVTVANA
Probe #653
5FAM-GGDIVTVANAK(CPQ2)-kk-PEG2-C
1328





654
DLGLKSVP
Probe #654
5FAM-GGDLGLKSVPK(CPQ2)-kk-PEG2-C
1329





655
DVMASNKR
Probe #655
5FAM-GGDVMASNKRK(CPQ2)-kk-PEG2-C
1330





656
ESDELNTI
Probe #656
5FAM-GGESDELNTIK(CPQ2)-kk-PEG2-C
1331





657
FHPLHSKI
Probe #657
5FAM-GGFHPLHSKIK(CPQ2)-kk-PEG2-C
1332





658
HARLVHV
Probe #658
5FAM-GGGHARLVHVK(CPQ2)-kk-PEG2-C
1333





659
HIANVERV
Probe #659
5FAM-GGHIANVERVK(CPQ2)-kk-PEG2-C
1334





660
KAAATQKK
Probe #660
5FAM-GGKAAATQKKK(CPQ2)-kk-PEG2-C
1335





661
LATASTMD
Probe #661
5FAM-GGLATASTMDK(CPQ2)-kk-PEG2-C
1336





662
LGPKGQT
Probe #662
5FAM-GGLGPKGQTGK(CPQ2)-kk-PEG2-C
1337





663
LSLPETGE
Probe #663
5FAM-GGLSLPETGEK(CPQ2)-kk-PEG2-C
1338





664
NLAGILKE
Probe #664
5FAM-GGNLAGILKEK(CPQ2)-kk-PEG2-C
1339





665
NPGMSEPV
Probe #665
5FAM-GGNPGMSEPVK(CPQ2)-kk-PEG2-C
1340





666
PFGCHAK
Probe #666
5FAM-GGPFGCHAKK(CPQ2)-kk-PEG2-C
1341





667
PLGLRWW
Probe #667
5FAM-GGPLGLRWWK(CPQ2)-kk-PEG2-C
1342





668
QMGVMQGV
Probe #668
5FAM-GGQMGVMQGVK(CPQ2)-kk-PEG2-
1343





C






669
QTCKCSCK
Probe #669
5FAM-GGQTCKCSCKK(CPQ2)-kk-PEG2-C
1344





670
QWAGLVEK
Probe #670
5FAM-GGQWAGLVEKK(CPQ2)-kk-PEG2-C
1345





671
RPAVMTSP
Probe #671
5FAM-GGRPAVMTSPK(CPQ2)-kk-PEG2-C
1346





672
TLRELHLD
Probe #672
5FAM-GGTLRELHLDK(CPQ2)-kk-PEG2-C
1347





673
TPPPSQGK
Probe #673
5FAM-GGTPPPSQGKK(CPQ2)-kk-PEG2-C
1348





674
TSEDLVVQ
Probe #674
5FAM-GGTSEDLVVQK(CPQ2)-kk-PEG2-C
1349





675
VWAAEAIS
Probe #675
5FAM-GGVWAAEAISK(CPQ2)-kk-PEG2-C
1350





676
R
Probe #676
H-R-AMC
1351





677
GC
Probe #677
FAM-GGC-PEG8
1352 





Nle = norleucine


K(FAM) = carboxy-fluorescein-L-lysine


HomoPhe = Hfe = L-homophenylalanine


Cys(OMeBzl) = C(OMeBzl) = S-para-methoxybenzyl cysteine


DIle = d-isoleucine


DArg = D-arginine


DVal = D-valine


Pyr = pyroglutamic acid


Cit = citrulline


C(Bzl) = S-benzyl-L-cysteine


Glu(OBzl) = benzyl-L-glutamate


Anb = amino-n-butyric acid


Gnf = guamidine-L-phenylalanine


K(Dnp) = dinitrobenzylation of lysine


His(Bzl) = benzyl-L-histidine


Tle = L-tert-leucine


Met(O) = L-methionine-sulfoxide


Bz = Benzoyl


Oic = L-octahydroindole-2-carboxylic acid


Nva = norvaline (click to see farther down list)


DThr = d-threonine


Phe(F5) = 2,3,4,5,6-pentafluoro-L-penylalanine


Phe(3Cl) = 3-chloro-L-phenylalanine


hSer(Bzl) = benzyl homoserine


hCha = homocyclohexylalnine


Phe(guan) = phenylalanine derivative with a guanidine group in the para position


Nle(O-Bzl) = Nle(OBz) = benzyloxy-L-norleucine


Met(O)2 = L-methionine sulfone


Dap = 2,3-diaminopropionic acid


hSer = homoserine


Met(O2) = methylsulfonylbutanoic acid


Abu = L-alpha-aminobutyric acid


Cha = L-cyclohexylalanine


Cys(Me) = L-Methyl cysteine


Orn = L-Ornithine


hF = L-Homophenylalanine


GABA = gamma aminobutyric acid


Pip = piperidine carboxylic acid


lower case = D-amino acids






The peptide linkers described herein for endoproteases may follow a design: XmAYn or AXnB, wherein respectively, A is a single amino acid and A and B are amino acid pairs recognized by a particular endoprotease, X and Y are any amino acid labeled or not with a reporter, and m, n are zero or any integer. This design is for exemplification only and should not be construed as the only possible design for the peptide linker.


The peptide linkers described herein for exoproteases may follow a design: XmAYn, wherein A is amino acid pairs recognized by a particular exoprotease, X and Y are any amino acid labeled or not with a reporter, and n is zero or any integer. This design is for exemplification only and should not be construed as the only possible design for the peptide linker.









TABLE 2







Exemplary peptide linker designs.

























Critical


amino
amino
amino
amino
amino




amino


acid 
acid
acid
acid
acid 
Example
Example
SEQ

acid


in
in
in
in
in
probe
Prob
ID
Protease
(single


P1′
P1
P2
P3
P4
name
design
NO
family
or pair)




















R/K



Probe
(FAM)-GWYKTQYGK
1353
Endo
Single







#161
(CPQ2)-NH2









R/K



Probe
(FAM)-GFARRWGGK
1354
Endo
Single







#109
(CPQ2)-PEG2-k-NH2









F/Y/LAV



Probe
(FAM)-GSYWP(Nle)QGK
1355
Endo
Single







#165
(CPQ2)-PEG2-k-NH2









F/Y



Probe
(FAM)-GFIY(Nle)PTGK
1356
Endo
Single







#140
(CPQ2)-PEG2-k-NH2









P



Probe
(FAM)-GTGPKGNGK
825
Endo
Single







#148
(CPQ2)-NH2








F
K



Probe
(FAM)-
894
Endo
Pair







#217
GWSKFW(Nle)GK(CPQ2)


(AB)





D
G



Probe
(FAM)-GKTGDARGK
871
Endo
Pair







#194
(CPQ2)-PEG2-k-NH2


(AB)





L
P



Probe
(FAM)-GGHPLSPGK
952
Endo
Pair







#275
P(CPQ2)-EG2-kk-NH2


(AB)






D
T/I/V


Probe
(FAM)-GVIDKDFGK
1357
Endo
Pair







#297
N(CPQ2)-H2


(AB)






R
K/R


Probe
(FAM)-GFARRWGGK
1358
Endo
Pair







#109
(CPQ2)-PEG2-k-NH2


(AB)





S
R



Probe
(FAM)-GPVRSTNGK
881
Endo
Pair







#204
(CPQ2)-NH2


(AB)






D

E

Probe
(FAM)-GENDRLPGK
876
Endo
Pair







#199
(CPQ2)-NH2


(near











neighbor











AXB)






D

V

Probe
(FAM)-GQWVDEDGK
925
Endo
Pair







#248
(CPQ2)-PEG2-k-NH2


(near











neighbor











AXXB)






K/R at



Probe
(FAM)-KGEFVHNPK
1359
Exo
Single



C-



#321
(CPQ2)K-OH






terminus














K/R/H at



Probe
(FAM)-GNAYNEIK
1360
Exo
Single



C-



#315
(CPQ2)R-OH






terminus














W/G/F



Probe
NH2-
1361
Exo
Single



at N-



#346

WK(FAM)NAGSKFG







terminus




kK(CPQ2)-NH2









Q/Kat



Probe
NH2-QK(FAM)KRV
1362
Exo
Single



N-



#362
QFLGK(CPQ2)-






terminus




NH2









In some embodiments, the cleavable linker may be a carbohydrate. Tung et al. reported a conjugate of β-galactoside and 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one), which has far-red fluorescence properties after a cleavage by 0-galactosidase. Tung C H, Zeng Q, Shah K, Kim D E, Schellingerhout D, Weissleder R. In vivo imaging of beta-galactosidase activity using far red fluorescent switch. Cancer Res. 2004 Mar. 1; 64(5):1579-83. Ho et al. reported combining β-galactosidase substrate with p-benzyloxycarbonyl as a self-immolative linker. β-D-Galactopyranoside, the substrate of 0-galactosidase, was conjugated to an optical probe through a para-substituted benzyloxycarbonyl group (serves as a first self-immolative linker) and a glycine residue (serves as a quencher and a second self-immolative linker). Enzymatic cleavage of the β-D-Galactopyranoside triggered a series of spontaneous reactions that resulted in a release of optically active probe. Ho, N.-H., Weissleder, R. and Tung, C.-H. (2007), A Self-Immolative Reporter For β-Galactosidase Sensing. ChemBioChem, 8: 560-566. Some carbohydrate linkers are commercially available.


In some embodiments, the cleavable linker may be a nucleic acid. The effect of a DNA linker on the behavior of its conjugate both reduces the toxicity of the free drug by reducing its cell penetration, which is positive in case of premature deconjugation in the bloodstream and increases the off-target toxicity on low antigen-expressing cells, presumably due to nonspecific interaction of the nucleic acid-based linker with the cell surface. For example, in an antibody-drug conjugates, the antibody and drug can be non-covalently connected using complementary DNA linkers. Dovgan, I., Ehkirch, A., Lehot, V. et al. On the use of DNA as a linker in antibody-drug conjugates: synthesis, stability and in vitro potency. Sci Rep 10, 7691 (2020). Dovgan et al. disclosed a trastuzumab to be connected to monomethyl auristatin E (MMAE) through a 37-mer oligonucleotide.


In some embodiments, the cleavable linker may be a lipid. In some embodiments, the cleavable linker may be a phospholipid. The insertion of phospholipid groups between two fluorescent dyes or a dye/quencher pair allows the detection of phospholipase cleavage activity. In some embodiments, the cleavable linker may be a phosphodiester. The insertion of phosphodiester groups between two fluorescent dyes or a dye/quencher pair allows the detection of phosphodiesterase cleavage activity. In some embodiments, the lipid is directly attached to the fluorophore: once the covalent bond between the lipid and fluorophore is cleaved, the increase of fluorescent activity allows for the detection of the enzyme presence


In some embodiments, the cleavable linker may be an ester. Ester groups are often cleaved by saponification. The reactivity of the ester to cleavage can be enhanced by the use of electron-withdrawing groups or stabilized by the use of auto-immolative spacers to precluded spontaneous hydrolysis. In chemical biology, ester-based cleavable compounds were initially used for protein purification and in structural biology. FRET-based probes were designed to image esterase activities.


In some embodiments, the cleavable linker may be a glycoside. For example, cellulase enzymes deconstruct cellulose to glucose, and are often comprised of glycosylated linkers connecting glycoside hydrolases (GHs) to carbohydrate-binding modules (CBMs).


In some embodiments, the cleavable linker may be a nucleophile/base sensitive linker. These can include, but are not limited to, halogen nucleophiles, oxygen nucleophiles, safety-catch linkers, thiol nucleophiles, nitrogen nucleophiles, and phenacyl ester derivatives.


In some embodiments, the cleavable linker may be sensitive to activity from all enzyme families, including but is not limited to oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases.


Fluoridolyzable linkers are widely used in organic chemistry as silicon-based protecting groups for alcohols. The high thermodynamic affinity of fluorine for silicon allows their removal in orthogonal and mild conditions using a fluorine source. In this reaction a fluoride ion reacts with silicon as nucleophilic species and the cleavage conditions depend on the steric hindrance of the silicon's alkyl group. Fluoride ions can also trigger bond cleavage due to their basic properties.


Oxygen nucleophiles include sulfone and ester linkers while safety-catch linkers allow greater control over the timing of the bond breakage, because the linker will remain stable until it is activated for cleavage by a chemical modification.


In secondary amine synthesis or solid phase synthesis, nitrobenzenesulfonamides are known to be cleaved with a thiol nucleophile, like b-mercaptoethanol. Cysteines can be modified by electron-deficient alkynes to form a vinyl sulfide linkage.


Displacement reactions involving a specific nitrogen species as a nucleophile can occur in mild cleavable conditions. These reactions can be classified into two groups; cleavage by aminolysis or exchange reaction. For aminolysis cleavage, examples include the cleavage of a malondialdehyde (MDA) indole derivative by either pyrrolidine or hydrazine, and the cleavage of an ester linker by hydroxylamine or hydrazine. Acylhydrazones 44 and hydrazones 45, 156 can be used as cleavable linkers through transimination in a mildly acidic medium. An amine catalyst (e.g., aniline, p-anisidine or hydroxylamine) accelerates hydrolysis and enables the effective transition between stable and dynamic states, which is required for cleavage and exchange.


In some embodiments, the cleavable linker may be a reduction sensitive linker. Reduction sensitive linkages have been used in chemical biology for a long time and it is a commonly used class of cleavable linker. Examples of cleavable linkers sensitive to reductive conditions include: nitroreductases, disulfide bridges and azo compounds. Karan et al. reported a fluorescent probe to detect nitroreductase. Sanu Karan, Mi Young Cho, Hyunseung Lee, Hwunjae Lee, Hye Sun Park, Mahesh Sundararajan, Jonathan L. Sessler, and Kwan Soo Hong. Near-Infrared Fluorescent Probe Activated by Nitroreductase for In Vitro and In Vivo Hypoxic Tumor Detection. Journal of Medicinal Chemistry 2021 64 (6), 2971-2981. In naturally occurring proteins, disulfide bridges generally play a role in maintaining the protein structure. They are known to be efficiently and rapidly cleaved by mild reducing agents like dithiothreitol (DTT), b-mercaptoethanol or tris(2-carboxyethyl)phosphine (TCEP). In chemical biology, disulfide bridges have been used in a wide range of applications including functional and structural proteomics, drug delivery, tumor imaging, DNA and protein-DNA complex purifications. The disulfide-based cleavable linker is commonly used due to its straightforward synthesis and rapid cleavage. Azo linkers are very appealing to chemical biologists since they are able to undergo cleavage following treatment with sodium dithionite, a mild and potentially bio-orthogonal reducing agent. The azo compound is reduced into two aniline moieties via an electrochemical reduction mechanism and this allows the use of reducing agents that are commonly used in many biological protocols, such as TCEP, DTT. In chemical biology, azo compounds have been used to cross-link proteins for over a decade and more recently for protein affinity purification.


In some embodiments, the cleavable linker may be an electrophile/acid sensitive linker. Acid sensitive linkers can be combined with other type of linkers. For example, a first β-galactosidase cleavage of the β-D-Galactopyranoside triggers the self-immolation of a benzyloxycarbonyl group, resulting in a release of optically active probe. Ho, N.-H., Weissleder, R. and Tung, C.-H. (2007), A Self-Immolative Reporter For β-Galactosidase Sensing. ChemBioChem, 8: 560-566. Two different modes of electrophilic cleavage are used in chemical biology: acidic sensitive linkers that are sensitive to proton sources, and alkyl 2-(diphenylphosphino)benzoate derivatives sensitive to azide compounds. Proton sensitive bonds are among the most frequently used cleavable functions in organic chemistry; illustrated by the development of the BOC group which protects amines, or the Merrifield resin used in solid phase synthesis. In organic chemistry, the cleavage conditions that can be tolerated are very flexible regarding the acids” reagents, solvents, temperatures and pH. In contrast, biocompatible acid cleavable linkers must be responsive to minor changes in pH. Strong acidic conditions can lead to the denaturation of proteins and DNA. Biocompatible acid cleavable linkers are chosen for their instability near physiological pH and are often different from the classical protecting groups, which are cleaved with strong acids. Chemical reactions that can break or form bonds in water can be used as the basis of a cleavable linker, for example the Staudinger ligation. This reaction is proceeded by the nucleophilic attack of an alkyl 2-(diphenylphosphino)benzoate derivative on an azide, to form an aza-ylide intermediate. Then the ester traps the aza-ylide, which leads to the formation of an amide. In this process, the ester acts as a cleavable linker, and the azide as a bioorthogonal chemical agent, which guarantees a chemoselective and bioorthogonal cleavage.


In some embodiments, the cleavable linker may be a metal cleavable linker. Organometallic compounds are used to catalyze the modification of proteins containing non-natural amino acids, but their use as cleavage reagent in chemical biology has only been reported a few times. The allyl function is a commonly used protecting group for alcohols in organic synthesis and it is also used as a cleavable linker in DNA sequencing by synthesis Metal cleavable linkers were also used in the design of peptide nucleic acids (PNAs), which were developed for enzyme-independent DNA/RNA hybridization methods.


In some embodiments, the cleavable linker may be an oxidation sensitive linker. Sodium periodate is undoubtedly the most frequently used biocompatible oxidizing agent due to its ability to cleave vicinal diols to form two aldehydes compounds. One example of this type of cleavable linker consists of a vicinal diol with a tartaric acid spacer and two functional groups at both ends. Selenium based linkers also contain cleavable bonds sensitive to oxidizing agents, such as sodium periodate or N-chlorobenzenesulfonamide immobilized on polystyrene beads (iodo-beads). The trigger agent oxidizes the labile bond to selenium oxide, which is then cleaved directly via intramolecular b-elimination or rearrangement.


Reporter and Detection Methods


In some aspects, the probe/molecule described herein comprises a reporter. The reporter as described herein may be in any structure that may be capable of being detected by any method, including but not limited to fluorescent detection, spectroscopic detection, immunological detection or imaging detection. In some embodiments, the reporter may be a fluorescent label, a mass tag or a nucleic acid barcode.


In some embodiments, the reporter may be a fluorescent label. Labels, tags and probes containing small compounds such as florescence can be used to label proteins and nucleic acids. Bio-affinity towards other molecules (biotin, digoxygenin), enzymatic (AP, HRP) or chemiluminescent (esters or acridine) can be used as well. Genetically encoded markers like the fluorescent proteins of the GFP family have become a reporter of choice for gene expression studies and protein localization. In combination with subcellular tags, GFP can be used to label subcellular structures like synapses allowing novel approaches to study developmental processes like synapse formation. Other fluorescent labels include but are not limited to small organic dyes and lipophilic dyes. The fluorescence label may serve itself as the activity substrate without addition of linkers.


Some reporters are “internally quenched”, thus does not require a quencher, wherein the cleavage of a bond linking the internally quenched fluorophore to the substrate linker directly yields a fluorescent molecule. Many described probes for proteases, esterases, peroxidases and others function this way.


In some embodiments, the reporter may be a mass tag. Mass tag reagents are designed to enable identification and quantitation of proteins in different samples using mass spectrometry (MS). Mass tagging reagents within a set typically have the same nominal mass (i.e., are isobaric) and chemical structure composed of an amine-reactive NHS ester group, a spacer arm (mass normalizer), and a mass reporter.


In some embodiments, the reporter may be a nucleic acid barcode. For example, DNA barcoding is a system for species identification focused on the use of a short, standardized genetic region acting as a “barcode” in a similar way that Universal Product Codes are used by supermarket scanners to distinguish commercial products.


In some embodiments, the reporter may be detected using a ligand binding assay. A ligand binding assay often involves a detection step, such as an ELISA, including fluorescent, colorimetric, bioluminescent and chemiluminescent ELISAs, a paper test strip or lateral flow assay, or a bead-based fluorescent assay. In some embodiments, a paper-based ELISA test may be used to detect the cleaved reporter in the fluid sample. The paper-based ELISA may be created inexpensively, such as by reflowing wax deposited from a commercial solid ink printer to create an array of test spots on a single piece of paper. When the solid ink is heated to a liquid or semi-liquid state, the printed wax permeates the paper, creating hydrophobic barriers. The space between the hydrophobic barriers may then be used as individual reaction wells. The ELISA assay may be performed by drying the detection antibody on the individual reaction wells, constituting test spots on the paper, followed by blocking and washing steps. Fluid from a sample taken from the subject may then be added to the test spots. Then, for example, a streptavidin alkaline phosphate (ALP) conjugate may be added to the test spots, as the detection antibody. Bound ALP may then be exposed to a color reacting agent, such as BCIP/NBT (5-bromo-4-chloro-3″-indolyphosphate p-toluidine salt/nitro-blue tetrazolium chloride), which causes a purple colored precipitate, indicating presence of the reporter.


In some embodiments, the reporter can be detected using volatile organic compounds. Volatile organic compounds may be detected by analysis platforms such as gas chromatography instrument, a breathalyzer, a mass spectrometer, or use of optical or acoustic sensors. Gas chromatography may be used to detect compounds that can be vaporized without decomposition (e.g., volatile organic compounds). A gas chromatography instrument includes a mobile phase (or moving phase) that is a carrier gas, for example, an inert gas such as helium or an unreactive gas such as nitrogen, and a stationary phase that is a microscopic layer of liquid or polymer on an inert solid support, inside a piece of glass or metal tubing called a column. The column is coated with the stationary phase and the gaseous compounds analyzed interact with the walls of the column, causing them to elute at different times (i.e., have varying retention times in the column). Compounds may be distinguished by their retention times.


Mass spectrometry and enrichment/chromatography methods may be used to separate and distinguish/detect cleaved from intact reporters used in the present invention based on differences in mass and or presence of a label. For example, enzymatic reactions can result in the fragmentation of a parent molecule resulting in a mass shift of the starting substrate, this can be exploited in different chromatography/enrichment methods such as size exclusion chromatography and affinity enrichments. In mass spectrometry, a sample is ionized, for example by bombarding it with electrons. The sample may be solid, liquid, or gas. By ionizing the sample, some of the sample's molecules are broken into charged fragments. These ions may then be separated according to their mass-to-charge ratio. This is often performed by accelerating the ions and subjecting them to an electric or magnetic field, where ions having the same mass-to-charge ratio will undergo the same amount of deflection. When deflected, the ions may be detected by a mechanism capable of detecting charged particles, for example, an electron multiplier. The detected results may be displayed as a spectrum of the relative abundance of detected ions as a function of the mass-to-charge ratio. The molecules in the sample can then be identified by correlating known masses, such as the mass of an entire molecule to the identified masses or through a characteristic fragmentation pattern.


When the reporter includes a nucleic acid, the reporter may be detected by various sequencing methods known in the art, for example, traditional Sanger sequencing methods or by next-generation sequencing (NGS). NGS generally refers to non-Sanger-based high throughput nucleic acid sequencing technologies, in which many (i.e., thousands, millions, or billions) of nucleic acid strands can be sequenced in parallel. Examples of such NGS sequencing includes platforms produced by Illumina (e.g., HiSeq, MiSeq, NextSeq, MiniSeq, and iSeq 100), Pacific Biosciences (e.g., Sequel and RSII), and Ion Torrent by ThermoFisher (e.g., Ion S5, Ion Proton, Ion PGM, and Ion Chef systems). It is understood that any suitable NGS sequencing platform may be used for NGS to detect nucleic acid of the detectable analyte as described herein.


Analysis may be performed directly on the biological sample or the detectable cleaved reporters may be purified to some degree first. For example, a purification step may involve isolating the detectable analyte from other components in the biological sample. Purification may include methods such as affinity chromatography. The isolated or purified detectable analyte does not need to be 100% pure or even substantially pure prior to analysis. Detecting the cleaved reporters may provide a qualitative assessment (e.g., whether the detectable cleaved reporters, and thus the predetermined protease is present or absent) or a quantitative assessment (e.g., the amount of the detectable cleaved reporters present) to indicate a comparative activity level of the predetermined proteases in the fluid sample. The quantitative value may be calculated by any means, such as, by determining the percent relative amount of each fraction present in the sample. Methods for making these types of calculations are known in the art.


The cleaved reporters may be detected by any detection method that may be suitable for the particular reporter. In some aspects, the detection method comprises fluorescent detection, spectroscopic detection, mass spectrometry, immunological detection or imaging detection. In some aspects, the detection method may be fluorescence resonance energy transfer (FRET).


In some embodiments, the detection method may be spectroscopic detection. Spectroscopic methods of detection are very commonly employed in ion chromatography (IC) and are second only to conductivity detection in their frequency of usage. These methods can be divided broadly into the categories of molecular spectroscopic techniques and atomic spectroscopic techniques. Molecular spectroscopy includes UV-visible spectrophotometry, refractive index measurements, and photoluminescence techniques (fluorescence and phosphorescence). Atomic spectroscopy includes atomic emission spectroscopy (using various excitation sources) and atomic absorption spectroscopy. Many of the spectroscopic detection methods can operate in a direct or indirect mode. The definitions of these terms are the same as those used to describe the electrochemical detection modes. That is, direct spectroscopic detection results when the solute ion has a greater value of the measured detection parameter than does the eluent ion. Indirect detection results when the reverse is true.


In some embodiments, the detection method may be mass spectrometry. Mass spectrometry (MS) is an analytical technique that is used to measure the mass-to-charge ratio of ions. The results are typically presented as a mass spectrum, a plot of intensity as a function of the mass-to-charge ratio.


In some embodiments, the detection method may be fluorescence resonance energy transfer (FRET). FRET (Fluorescence Resonance Energy Transfer) is a distance dependent dipole-dipole interaction without the emission of a photon, which results in the transfer of energy from an initially excited donor molecule to an acceptor molecule. It allows the detection of molecular interactions in the nanometer range. FRET peptides are labeled with a donor molecule and an acceptor (quencher) molecule. In most cases, the donor and acceptor pairs are two different dyes. The transferred energy from a fluorescent donor is converted into molecular vibrations if the acceptor is a non-fluorescent dye (quencher). When the FRET is terminated (by separating donor and acceptor), an increase of donor fluorescence can be detected. When both the donor and acceptor dyes are fluorescent, the transferred energy is emitted as light of longer wavelength so that the intensity ratio change of donor and acceptor fluorescence can be measured. In order for efficient FRET quenching to take place, the fluorophore and quencher molecules must be close to each other (approximately 10-100 Å) and the absorption spectrum of the quencher must overlap with the emission spectrum of the fluorophore.


Precipitating Fluorophore


In some aspects, the cleaved reporter may be a precipitating fluorophore. In some embodiments, the precipitating fluorophore may be HPQ, Cl-HPQ, HTPQ, HTPQA, HBPQ, or HQPQ.


In some embodiments, the precipitating fluorophore may be HPQ, also known as 2-(2″-hydroxyphenyl)-4(3H)-quinazolinone. HPQ is a small organic dye known for its classic luminescence mechanism through excited-state intramolecular proton transfer (ESIPT), shows strong light emission in the solid state, but no emission in solution. HPQ is found to be strictly insoluble in water and exhibits intense solid-state fluorescence similar to that of tetraphenyl ethylene. Moreover, its essential properties of insolubility and intense solid-state fluorescence can be countered and reversed, by prohibiting the establishment of an internal hydrogen bond between the imine nitrogen and phenolic hydroxyl group.


In some embodiments, the precipitating fluorophore may be Cl-HPQ. Cl-HPQ is released when HPQF, a water soluble and non-fluorescent molecule, reacts with furin. Cl-HPQ starts to precipitate near the enzyme activity site, and the precipitates emit bright solid-state fluorescence with more than 60-fold fluorescence enhancement. Li et al. In Situ Imaging of Furin Activity with a Highly Stable Probe by Releasing of Precipitating Fluorochrome. Anal. Chem. 2018, 90, 19, 11680-11687.


In some embodiments, the precipitating fluorophore may be HTPQ. HTPQ is found to be strictly insoluble in water and shows intense fluorescence in the solid state with maximum excitation and emission wavelengths at 410 nm and 550 nm respectively. This makes it far better suited to the use with a confocal microscope. The large Stokes shift of HTPQ contributes additional and highly desirable advantages: increased sensitivity, minimized background fluorescence and enhanced bioimaging contrast. Liu et al. In Situ Localization of Enzyme activity in Live Cells by a Molecular Probe Releasing a Precipitating Fluorochrome. Angew Chem Int Ed Engl. 2017 Sep. 18; 56(39):11788-11792.


In some embodiments, the precipitating fluorophore may be HTPQA. HTPQA is another enzyme-responsive fluorogenic probe derived from HTPQ. When converted by ALP, the probe releases free HTPQ which starts to precipitate after a very short delay; the precipitate emits bright solid-state fluorescence with more than 100-fold fluorescence enhancement.


In some embodiments, the precipitating fluorophore may be HBPQ. HBPQ is completely insoluble in water and shows strong yellow solid emission when excited with a 405 nm laser. Liu et al. Precipitated Fluorophore-Based Molecular Probe for In Situ Imaging of Aminopeptidase N in Living Cells and Tumors. Anal. Chem. 2021, 93, 16, 6463-6471, Publication Date: Apr. 14, 2021.


In some embodiments, the precipitating fluorophore may be HQPQ. HQPQ is, a novel solid-state fluorophore that is insoluble in water. Li et al. Precipitated Fluorophore-Based Probe for Accurate Detection of Mitochondrial Analytes. Anal. Chem. 2021, 93, 4, 2235-2243. Publication Date: Jan. 5, 2021.


The precipitating and non-precipitating fluorophores can be separated from the enzyme substrate by a self-immolative substrate to stabilize the initial probe and ensure that the enzymatic cleavage is transduced via the immolative spacer into the formation of the precipitating fluorophore or the non-internally quenched soluble fluorophore.


Fluorescent Quencher


In some aspects, the probe/molecule described herein comprises a fluorescent quencher. The fluorescent quencher as described herein may be in any structure that is capable of decreasing the fluorescence intensity of a given substance. In some embodiments, the fluorescent quencher may be BHQ0, BHQ1, BHQ2, BHQ3, BBQ650, ATTO 540Q, ATTO 580Q, ATTO 612Q, CPQ2, QSY-21, QSY-35, QSY-7, QSY-9, DABCYL (4-([4′-dimethylamino)phenyl]azo)benzoyl), Dnp (2,4-dinitrophenyl) or Eclipse®.


In some embodiments, the fluorescent quencher may be a BHQ quencher including, but not limited to, BHQ0, BHQ1, BHQ2, BHQ3, or BBQ650. BHQ, or black hole quencher, dyes work through a combination of FRET and static quenching to enable avoidance of the residual background signal common to fluorescing quenchers such as TAMRA, or low signal-to-noise ratio. The different types of BHQ dyes are used to quench different colored dyes with BHQ1 used to quench green and yellow dyes such as FAM, TET, or HEX and BHQ2 used for quenching orange and red dyes. BHQ dyes are true dark quenchers with no native emission due to their polyacromatic-azo backbone. Substituting electron-donating and withdrawing groups on the aromatic rings produces a complete series of quenchers with broad absorption curves that span the visible spectrum.


In some embodiments, the fluorescent quencher may be an ATTO quencher including, but not limited to ATTO 540Q, ATTO 580Q, or ATTO 612Q. ATTO quenchers have characteristic properties of strong absorption (high extinction coefficient) and high photo-stability. ATTO quenchers are often utilized as fluorescent quenchers on amine-labeled nucleotides for FRET experiments.


In some embodiments, the fluorescent quencher may be CPQ2. The quencher CPQ2 is often used as a pair with the fluorescent donor 5-carboxylfluorescein.


In some embodiments, the fluorescent quencher may be a QSY quencher including but not limited to QSY-21, QSY-35, QSY-7, or QSY-9. QSY probes are dark quenchers, substances that absorb excitation energy from a fluorophore and dissipate the energy as heat.


In some embodiments, the fluorescent quencher may be DABCYL (4-([4′-dimethylamino)phenyl]azo)benzoyl). DABCYL is one of the most popular acceptors for developing FRET-based nucleic acid probes and protease substrates. DABCYL dyes are often paired with EDANS in FRET-based fluorescent probes. DABCYL has a broad and intense visible absorption but no fluorescence.


In some embodiments, the fluorescent quencher may be Dnp (2,4-dinitrophenyl). Dnp is a stable quencher and its absorption spectrum does not change with pH, which makes this group a convenient marker for substrate quantitation in solutions.


In some embodiments, the fluorescent quencher may be Eclipse®. Eclipse® is a non-fluorescent chromophore and a dark quencher often used in dual-labelled probes. As dark quenchers, Eclipse® absorbs energy without emitting fluorescence. Eclipse® has an absorption range from 390 nm to 625 nm and is capable of effective performance in a wide range of colored FRET probes.


Carrier


In some aspects, the probe/molecule described herein comprises a carrier. The fluorescent quencher as described herein may be in any structure. In some embodiments, the carrier may be a native, labeled or synthetic protein, a synthetic chemical polymer of precisely known chemical composition or with a distribution around a mean molecular weight (e.g. a linear or branched PEG polymers), an oligonucleotide, a phosphorodiamidate morpholino oligomer (PMO), or a foldamer, a lipid, a lipid micelle, a nanoparticle (e.g., iron oxide, gold, and non-metallic nanoparticles), a solid support made of polystyrene, polypropylene or any other type of plastic or polymer. In some embodiments, the carrier may be a peptide longer than the peptide linker. A carrier can be covalently or non-covalently attached to the cleavable linker.


In some embodiments, the carrier may be a nanoparticle. The transport of insoluble drugs via nanoparticles is improving because of their small particle size. Nanoparticle carrier is a kind of sub-micro particle delivery system, which belongs to a nanoscale microscope. Drugs encapsulated in sub-particles can adjust the speed of drug release, increase the permeability of biofilm, change the distribution in vivo, and improve the bioavailability. Nanoparticles are solid colloidal particles ranging in size from 10 to 100 nm used as a core in functionalization systems. They are generally composed of natural or synthetic macromolecule substances and can be used as carriers for conducting or transporting drugs. Nanospheres and nanocapsules can be formed. The chemical materials of nanomaterials are chitosan, gelatin, branched polymers, carbon-based carriers, etc. Gold nanoparticles consist of a core of gold atoms that can be functionalized by addition of a monolayer of moieties containing a thiol (SH) group.


In some embodiments, the carrier may be a native, labeled or synthetic protein. Proteins can be used as carriers for the delivery of chemicals and biomolecular drugs, such as anticancer drugs and therapeutic proteins. Protein nanoparticles have several advantages as a drug delivery system, such as biodegradability, stability, surface modification of particles, ease of particle size control, and they have less problems associated with toxicity issues, such as immunogenicity. Protein nanoparticles can be generated using proteins, such as fibroins, albumin, gelatin, gliadine, legumin, 30Kc19, lipoprotein, and ferritin proteins, and are prepared through emulsion, electrospray, and desolvation methods. Hong S, Choi D W, Kim H N, Park C G, Lee W, Park H H. Protein-Based Nanoparticles as Drug Delivery Systems. Pharmaceutics. 2020; 12(7):604. Published 2020 Jun. 29. For example, albumin, a plasma protein with a molecular weight of 66 kDa, has been extensively investigated as a drug carrier


In some embodiments, the carrier may be a synthetic chemical polymer. Polymeric nanoparticles have been extensively investigated as drug nanocarriers. Drug loading is achieved either by (i) entrapment of an aqueous drug phase using the polymer to form nanoscale structures such as cages and capsules or (ii) chemical linking of the drug molecules to the polymer backbone by means of a simple ester or amide bond that can be hydrolyzed in vivo. The most widely researched synthetic polymers include polylactide (PLA), poly(D,L-lactide-co-glycolide) (PLGA) and PEG. All three polymers are hydrolyzed in vivo and are biodegradable. Malam Y, Loizidou M, Seifalian A M. Liposomes and nanoparticles: nanosized vehicles for drug delivery in cancer. Trends Pharmacol Sci. 2009 November; 30(11):592-9.


In some embodiments, the carrier may be a polyethylene glycol (PEG). PEG has been studied comprehensively as a carrier because it is soluble in both organic and hydrophilic solvents. Unlike many other synthetic polymers, PEG is relatively hydrophilic. Conjugation with PEG increases the solubility of hydrophobic molecules and prolongs the circulation time in the organism. PEG also minimizes the nonspecific absorption of a molecule, such as a drug, provides specific affinity toward the targeted tissue, and increases the drug accumulation in malignant tissue. PEG can be conjugated to other polymers to make them less hydrophobic (i.e., PEGylation). The changes in surface hydrophilicity prevent protein adsorption, thereby enabling cell adhesion and proliferation on biomaterial scaffolds. The PMO backbone is made of morpholino rings with phosphorodiamidate linkage, which protects them from nuclease degradation while still maintaining the complementary base pairing. The potential application of PMO-based antisense technology targeting bacterial pathogens is being explored for the development of a new class of antibacterial drugs. Panchal R G, Geller B L, Mellbye B, Lane D, Iversen P L, Bavari S. Peptide conjugated phosphorodiamidate morpholino oligomers increase survival of mice challenged with Ames Bacillus anthracis. Nucleic Acid Ther. 2012; 22(5):316-322. Fluorescein-tagged Morpholinos combined with fluorescein-specific antibodies can be used as probes for in-situ hybridization to miRNAs.


In some embodiments, the carrier may be an oligonucleotide. Biostable, high-payload DNA nanoassemblies of various structures, including cage-like DNA nanostructure, DNA particles, DNA polypods, and DNA hydrogel, have been reported. Cage-like DNA structures hold drug molecules firmly inside the structure and leave a large space within the cavity. These DNA nanostructures use their unique structure to carry abundant CpG, and their biocompatibility and size advantages to enter immune cells to achieve immunotherapy for various diseases. Part of the DNA nanostructures can also achieve more effective treatment in conjunction with other functional components such as aPD1, RNA, TLR ligands. DNA-based nanoparticles, such as spherical nucleic acids, hybrid DNA-based nanoparticles, polypod-like DNA nanostructure, DNA hydrogels have been reported. Chi Q, Yang Z, Xu K, Wang C and Liang H (2020) DNA Nanostructure as an Efficient Drug Delivery Platform for Immunotherapy. Front. Pharmacol. 10:1585.


In some embodiments, the carrier may be a phosphorodiamidate Morpholino oligomer (PMO). Antisense phosphorodiamidate morpholino oligomers (PMOs) and their derivatives downregulate target gene expression in a sequence-dependent manner by interfering with the binding of ribosome to mRNA and thereby inhibiting protein translation.


In some embodiments, the carrier may be a lipid or a lipid micelle. The liposome bilayer can be composed of either synthetic or natural phospholipids. The predominant physical and chemical properties of a liposome are based on the net properties of the constituent phospholipids, including permeability, charge density and steric hindrance. The lipid bilayer closes in on itself due to interactions between water molecules and the hydrophobic phosphate groups of the phospholipids. This process of liposome formation is spontaneous because the amphiphilic phospholipids self-associate into bilayers. Drug loading into liposomes can be achieved through (i) liposome formation in an aqueous solution saturated with soluble drug; (ii) the use of organic solvents and solvent exchange mechanisms; (iii) the use of lipophilic drugs; and (iv) pH gradient methods. Malam Y, Loizidou M, Seifalian A M. Liposomes and nanoparticles: nanosized vehicles for drug delivery in cancer. Trends Pharmacol Sci. 2009 November; 30(11):592-9.


In some embodiments, the carrier may be a solid support made of polystyrene, polypropylene or any other type of plastic. For example, drug delivery properties of microporous polystyrene solid foams have been reported by Canal et al. These materials were obtained by polymerization in the continuous phase of highly concentrated emulsions prepared by the phase inversion temperature method. Their porosity, specific surface and surface topography are associated with drug incorporation and release characteristics. Canal, Cristina & Aparicio, Rosa & Vilchez, Alejandro & Esquena, Jordi & Garcia-Celma, Maria. (2012). Drug Delivery Properties of Macroporous Polystyrene Solid Foams. Journal of pharmacy & pharmaceutical sciences: a publication of the Canadian Society for Pharmaceutical Sciences, Société canadienne des sciences pharmaceutiques. 15. 197-207.


In some embodiments, the carrier may be a foldamer. Foldamer, is a folded oligomer or polymer with a well-defined conformation. The conformation of foldamers is highly predictable from their primary sequences, therefore, it is possible to arrange functional groups at target positions and it may be possible to design functional foldamers, such as for efficient cellular uptake. For example, Cell-penetrating peptide (CPP) foldamers are peptide-based foldamers equipped with cell membrane permeabilities. Peptide foldamers contain unnatural amino acids, non-proteinogenic amino acids, which make the peptide adopt a stable secondary structure, especially helical structures, even in short sequences. This property is helpful for the design of amphipathic CPPs with a stable helical structure. Furthermore, peptides containing unnatural amino acids generally exhibit resistance to hydrolysis by proteases, which are abundant throughout the body and in the cells. High stability of the peptide foldamers against enzymatic degradation can lead to their prolonged function in vivo. Makoto Oba, Cell-Penetrating Peptide Foldamers: Drug Delivery Tools. ChemBioChem 10.1002/cbic.201900204.


Self-Immolative Spacer


In some aspects, the probe/molecule described herein comprises a self-immolative spacer. In some embodiments, the self-immolative spacer comprise a disulfide, a p-amino benzyl alcohol, an a-quinone methide spacer, a hetheroaminebifuncional disulfide, a thiol-based pirydazinediones, a p-aminebenzyloxycarbonyl, a dipeptide, a Gly-Pro (SEQ ID NO: 530), a L-Phe-Sar, a trans-cyclooctene tetrazine, a ortho Hydroxy-protected Aryl sulfate, a phosphoramidate-based spacer, a hydroxybenzyl, a trimethyl carbamate, a quinone methide-based spacer, a cyclizing spacer, a Trimethyl lock, a 2-amino methyl piperidine or an ethylene diamine derived cyclizing spacer. Gonzaga et al. Perspective about self-immolative drug delivery systems. Journal of Pharmaceutical Sciences 109 (2020) 3262-3281.


Cleavage of the cleavable linker by a predetermined protease or enzyme makes the self-immolative spacer dissociate from the precipitating fluorescent or non-fluorescent reporter, thereby resulting in a detectable signal. The cleavable linker of the plurality of probes/molecules may be cleavable by a predetermined endoprotease in the body fluid sample resulting in auto immolation and reporter release or results in a protease substrate that can be cleaved by a predetermined exopeptidase. In some embodiments, the predetermined exopeptidase is added to the body fluid sample. In some embodiments, the predetermined exopeptidase cleaves the protease substrate, thereby causing the self-immolative spacer to dissociate from the precipitating fluorescent reporter, thereby resulting in a detectable signal.


Body Fluid Samples


Determination of the disease or condition is based on the rate of formation or amount of the released reporter detected in the body fluid sample. In some embodiments, the body fluid sample may be blood, serum, plasma, bone marrow fluid, lymphatic fluid, bile, amniotic fluid, mucosal fluid, saliva, urine, cerebrospinal fluid, synovial fluid, semen, ductal aspirate, feces, vaginal effluent, cyst fluid, tissue homogenate, tissue-derived fluid, lachrymal fluid and patient-derived cell line supernatant. In some embodiments, the body fluid sample comprises a rinse fluid. In some embodiments, the rinse fluid may be a mouthwash rinse, a bronchioalveolar rinse, a lavage fluid, a hair wash rinse, a nasal spray effluent, a swab of any bodily surface, orifice or organ structure applied to saline or any media or any derivatives thereof.


In some embodiments, the body fluid sample may be blood. Blood is a constantly circulating fluid providing the body with nutrition, oxygen, and waste removal. Blood is mostly liquid, with numerous cells and proteins suspended in it. Blood is made of several main factors including plasma, red blood cells, white blood cells, and platelets.


In some embodiments, the body fluid sample may be a plasma. Plasma is the liquid that remains when clotting is prevented with the addition of an anticoagulant. Serum is the conventional term in the art for the fluid that remains when clotting factors are removed from plasma. Anticoagulants are medicines that help prevent blood clots. Examples of anticoagulants include, but are not limited to, an ethylenediamine tetraacetic acid (EDTA), a citrate, a heparin, an oxalate, any salt, solvate, enantiomer, tautomer and geometric isomer thereof, or any mixtures thereof.


In some embodiments, the anticoagulant may be EDTA. The main property of EDTA, a polyprotic acid containing four carboxylic acid groups and two amine groups with lone pair electrons, is the ability to chelate or complex metal ions in 1:1 metal-EDTA complexes. Owing to its strong complexation with metal ions that are cofactors for enzymes, EDTA is widely used as a sequestering agent to prevent some enzyme reactions from occurring. When blood is collected with no additives within an appropriate container (blood tube), it clots fairly quickly. As calcium ions are necessary for this process, the specific association between the carboxylic groups of EDTA and calcium is a reliable solution to prevent clotting, stabilizing whole blood in a fluid form, as required for some laboratory analyses. Moreover, EDTA showed optimal extended stabilization of blood cells and particles. Three EDTA formulations can be employed as anticoagulants: Na2EDTA, K2EDTA and K3EDTA, choice of which mostly depends on the type of analyses to be performed.


In some embodiments, the anticoagulant may be a citrate. Citrate (C6H7O7) is a small negatively charged molecule with a molecular weight of 191 Daltons. Citrate can be used as the anticoagulant of choice for stored blood products, typically as acid citrate dextrose (ACD), (3.22% citrate, 112.9 mmol/l citrate, 123.6 mmol/l glucose, 224.4 mmol/l sodium and 114.2 mmol/l hydrogen ions), or trisodium citrate (TCA) Na3C3H5O(COO)3, (4% TCA, 136 mmol/l citrate, 420 mmol/l sodium). Citrate chelates calcium, and at a concentration of 4-6 mmol/l with an ionized calcium of <0.2 mmol/l prevents activation of both coagulation cascades and platelets. As such, citrate has been the standard anticoagulant used by hematologists and blood transfusion services for stored blood products and also as an extracorporeal anticoagulant for centrifugal platelet and leucopheresis techniques and plasma exchange.


In some embodiments, the anticoagulant may be a heparin. The molecular basis for the anticoagulant action of heparin lies in its ability to bind to and enhance the inhibitory activity of the plasma protein antithrombin against several serine proteases of the coagulation system, most importantly factors IIa (thrombin), Xa and IXa. Two major mechanisms underlie heparin's potentiation of antithrombin. The conformational changes induced by heparin binding cause both expulsion of the reactive loop and exposure of exosites of the surface of antithrombin, which bind directly to the enzyme target; and a template mechanism exists in which both inhibitor and enzyme bind to the same heparin molecule. The relative importance of these two modes of action varies between enzymes. In addition, heparin can act through other serine protease inhibitors such as heparin co-factor II, protein C inhibitor and tissue factor plasminogen inhibitor. The antithrombotic action of heparin in vivo, though dominated by anticoagulant mechanisms, is more complex, and interactions with other plasma proteins and cells play significant roles in the living vasculature.


In some embodiments, the anticoagulant may be an oxalate. Sodium, potassium, ammonium, and lithium oxalates inhibit blood coagulation by forming insoluble complex with calcium. Potassium oxalate at concentration of 1-2 mg/ml of blood is widely used. Combined ammonium and/or potassium oxalate does not cause shrinkage of erythrocytes. It consists of three parts by weight of ammonium oxalate, which causes swelling of the erythrocytes, balanced by two parts of potassium oxalate which causes shrinkage. NH4+ & K+ oxalate mixture in the ratio of 3:2, and 2 mg/ml of blood is the required amount.


In some embodiments, the body fluid sample may be bone marrow fluid. Bone marrow is found in the center of most bones and has many blood vessels. There are two types of bone marrow: red and yellow. Red marrow contains blood stem cells that can become red blood cells, white blood cells, or platelets. Yellow marrow is made mostly of fat.


In some embodiments, the body fluid sample may be lymphatic fluid. Lymphatic fluid, also called lymph, is a collection of the extra fluid that drains from cells and tissues, that is not reabsorbed into the capillaries.


In some embodiments, the body fluid sample may be bile. Bile is a digestive fluid produced by the liver and stored in the gallbladder. During bile reflux, digestive fluid backs up into the stomach and, in some cases, the esophagus.


In some embodiments, the body fluid sample may be amniotic fluid. Amniotic fluid is a clear, slightly yellowish liquid that surrounds the unborn baby (fetus) during pregnancy. It is contained in the amniotic sac.


In some embodiments, the body fluid sample may be mucosal fluid. Mucosal fluid, also called mucus, is a thick protective fluid that is secreted by mucous membranes and used to stop pathogens and dirt from entering the body. Mucus is also used to prevent bodily tissues from being dehydrated.


In some embodiments, the body fluid sample may be saliva. Saliva is an extracellular fluid produced and secreted by salivary glands in the mouth.


In some embodiments, the body fluid sample may be urine. Urine is a liquid by-product of metabolism in humans and in many other animals. Urine flows from the kidneys through the ureters to the urinary bladder.


In some embodiments, the body fluid sample may be cerebrospinal fluid. Cerebrospinal fluid is a clear fluid that surrounds the brain and spinal cord. It cushions the brain and spinal cord from injury and also serves as a nutrient delivery and waste removal system for the brain


In some embodiments, the body fluid sample may be synovial fluid. Synovial fluid, also known as joint fluid, is a thick liquid located between your joints. The fluid cushions the ends of bones and reduces friction when joints are moved.


In some embodiments, the body fluid sample may be semen. Semen is the male reproductive fluid which contains spermatozoa in suspension.


In some embodiments, the body fluid sample may be ductal aspirate. Ductal aspirate, also known as ductal lavage, ductal fluid, or lavage fluid, is fluid collected from a duct, such as the milk duct of the breast.


In some embodiments, the body fluid sample may be feces. Feces, also known as excrement or stool is waste matter discharged from the bowels after food has been digested.


In some embodiments, the body fluid sample may be vaginal effluent. Vaginal effluent, also known as vaginal discharge, is a clear or whitish fluid that comes out of the vagina.


In some embodiments, the body fluid sample may be lachrymal fluid. Lachrymal fluid, also known as lacrimal fluid, is secreted by the lacrimal glands to lubricate the eye and fight bacteria.


In some embodiments, the body fluid sample may be tissue homogenate. A tissue homogenate is obtained through mechanical micro-disruption of fresh tissue and the cell membranes are mechanically permeabilized.


Proteases and Other Agents


The probe/molecule described herein may be cleaved by a protease from the body fluid. In some embodiments, the protease comprises an endopeptidase or an exopeptidase.


In some embodiments, the protease comprises an endopeptidase. An endopeptidase is an enzyme which breaks peptide bonds other than terminal ones in a peptide chain.


In some embodiments, the protease comprises an exopeptidase. An exopeptidase is an enzyme that catalyzes the cleavage of the terminal or penultimate peptide bond; the process releases a single amino acid or dipeptide from the peptide chain.


In some embodiments, the protease comprises an A20 (TNFa-induced protein 3), an abhydrolase domain containing 4, an abhydrolase domain containing 12, an abhydrolase domain containing 12B, an abhydrolase domain containing 13, an acrosin, an acylaminoacyl-peptidase, a disintegrin and metalloproteinase (ADAM), an ADAM1a, an ADAM2 (Fertilin-b), an ADAM3B, an ADAM4, an ADAM4B, an ADAM5, an ADAM6, an ADAM7, an ADAM8, an ADAM9, an ADAM10, an ADAM11, an ADAM12 metalloprotease, an ADAM15, an ADAM17, an ADAM18, an ADAM19, an ADAM20, an ADAM21, an ADAM22, an ADAM23, an ADAM28, an ADAM29, an ADAM30, an ADAM32, an ADAM33, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), an ADAMTS1, an ADAMTS2, an ADAMTS3, an ADAMTS4, an ADAMTS5/11, an ADAMTS6, an ADAMTS7, an ADAMTS8, an ADAMTS9, an ADAMTS10, an ADAMTS12, an ADAMTS13, an ADAMTS14, an ADAMTS15, an ADAMTS16, an ADAMTS17, an ADAMTS18, an ADAMTS19, an ADAMTS20, an adipocyte-enh. binding protein 1, an Afg3-like protein 1, an Afg3-like protein 2, an airway-trypsin-like protease, an aminoacylase, an aminopeptidase A, an aminopeptidase B, an aminopeptidase B-like 1, an aminopeptidase MAMS/L-RAP, an aminopeptidase N, an aminopeptidase O, an aminopeptidase P homologue, an aminopeptidase P1, an aminopeptidase PILS, an aminopeptidase Q, an aminopeptidase-like 1, an AMSH/STAMBP, an AMSH-LP/STAMBPL1, an angiotensin-converting enzyme 1 (ACE1), an angiotensin-converting enzyme 2 (ACE2), an angiotensin-converting enzyme 3 (ACE3), an anionic trypsin (II), an apolipoprotein (a), an archaemetzincin-1, an archaemetzincin-2, an aspartoacylase, an aspartoacylase-3, an aspartyl aminopeptidase, an ataxin-3, an ataxin-3 like, an ATP/GTP binding protein 1, an ATP/GTP binding protein-like 2, an ATP/GTP binding protein-like 3, an ATP/GTP binding protein-like 4, an ATP/GTP binding protein-like 5, an ATP23 peptidase, an autophagin-1, an autophagin-2, an autophagin-3, an autophagin-4, an azurocidin, or a combination hereof.


In some embodiments, the protease comprises a beta lactamase, a beta-secretase 1, a beta-secretase 2, a bleomycin hydrolase, a brain serine proteinase 2, a BRCC36 (BRCA2-containing complex, sub 3), a calpain, a calpain 1, a calpain 2, a calpain 3, a calpain 4, a calpain 5, a calpain 6, a calpain 7, a calpain 7-like, a calpain 8, a calpain 9, a calpain 10, a calpain 11, a calpain 12, a calpain 13, a calpain 14, a calpain 15 (Solh protein), or a combination hereof.


In some embodiments, the protease comprises a cysteine protease, a carboxypeptidase A1, a carboxypeptidase A2, a carboxypeptidase A3, a carboxypeptidase A4, a carboxypeptidase A5, a carboxypeptidase A6, a carboxypeptidase B, a carboxypeptidase D, a carboxypeptidase E, a carboxypeptidase M, a carboxypeptidase N, a carboxypeptidase O, a carboxypeptidase U, a carboxypeptidase X1, a carboxypeptidase X2, a carboxypeptidase Z, a carnosine dipeptidase 1, a carnosine dipeptidase 2, a caspase recruitment domain family, member 8, a caspase, a caspase-1, a caspase-2, a caspase-3, a caspase-4/11, a caspase-5, a caspase-6, a caspase-7, a caspase-8, a caspase-9, a caspase-10, a caspase-12, a caspase-14, a caspase-14-like, a casper/FLIP, a cathepsin, a cathepsin A (CTSA), a cathepsin B (CTSB), a cathepsin C (CTSC), a cathepsin D (CTSD), a cathepsin E (CTSE), a cathepsin F, a cathepsin G, a cathepsin H (CTSH), a cathepsin K (CTSK), a cathepsin L (CTSL), a cathepsin L2, a cathepsin O, a cathepsin S (CTSS), a cathepsin V (CTSV), a cathepsin W, a cathepsin Z (CTSZ), a cationic trypsin, a cezanne/OTU domain containing 7B, a cezanne-2, a CGI-58, a chymase, a chymopasin, a chymosin, a chymotrypsin B, a chymotrypsin C, a coagulation factor IXa, a coagulation factor VIIa, a coagulation factor Xa, a coagulation factor XIa, a coagulation factor XIIa, a collagenase 1, a collagenase 2, a collagenase 3, a complement protease C1r serine protease, a complement protease C1s serine protease, a complement C1r-homolog, a complement component 2, a complement component C1ra, a complement component C1sa, a complement factor B, a complement factor D, a complement factor D-like, a complement factor I, a COPS6, a corin, a CSN5 (JAB1), a cylindromatosis protein, a cytosol alanyl aminopep.-like 1, a cytosol alanyl aminopeptidase, or a combination hereof.


In some embodiments, the protease comprises a DDI-related protease, a DECYSIN, a Der1-like domain family, member 1, a Der1-like domain family, member 2, a Der1-like domain family, member 3, a DESC1 protease, a desert hedgehog protein, a desumoylating isopeptidase 1, a desumoylating isopeptidase 2, a dihydroorotase, a dihydropyrimidinase, a dihydropyrimidinase-related protein 1, a dihydropyrimidinase-related protein 2, a dihydropyrimidinase-related protein 3, a dihydropyrimidinase-related protein 4, a dihydropyrimidinase-related protein 5, a DINE peptidase, a dipeptidyl peptidase (DPP), a dipeptidyl peptidase (DPP1), a dipeptidyl-peptidase 4 (DPP4), a dipeptidyl-peptidase 6 (DPP6), a dipeptidyl-peptidase 8 (DPP8), a dipeptidyl-peptidase 9 (DPP9), a dipeptidyl-peptidase II, a dipeptidyl-peptidase III, a dipeptidyl-peptidase 10 (DPP10), a DJ-1, a DNA-damage inducible protein, a DNA-damage inducible protein 2, a DUB-1, a DUB-2, a DUB2a, a DUB2a-like, a DUB2a-like2, a DUB6, or a combination hereof.


In some embodiments, the protease comprises an enamelysin, an endopeptidase C1p, an endoplasmic reticulum metallopeptidase 1, an endothelin-converting enzyme 1, an endothelin-converting enzyme 2, an enteropeptidase, an epidermis-specific SP-like, an epilysin, an epithelial cell transforming sequence 2 oncogene-like, an epitheliasin, an epoxide hydrolase, an epoxyde hydrolase related protein, an eukar. translation initiation F3SF, an eukar. translation initiation F3SH, or a combination hereof.


In some embodiments, the protease comprises a Factor VII activating protease, a FACE-1/ZMPSTE24, a FACE-2/RCE1, a family with sequence similarity 108, member A1, a family with sequence similarity 108, member B1, a family with sequence similarity 108, member C1, a family with sequence similarity 111, A, a family with sequence similarity 111, B, a furin, or a combination hereof.


In some embodiments, the protease comprises a gamma-glutamyl hydrolase, a gamma-glutamyltransferase 1, a gamma-glutamyltransferase 2, a gamma-glutamyltransferase 5, a gamma-glutamyltransferase 6, a gamma-glutamyltransferase m-3, a gamma-glutamyltransferase-like 3, a GCDFP15, a gelatinase A, a gelatinase B, a Gln-fructose-6-P transamidase 1, a Gln-fructose-6-P transamidase 2, a Gln-fructose-6-P transamidase 3, a Gln-PRPP amidotransferase, a glutamate carboxypeptidase II, a glutaminyl cyclase, a glutaminyl cyclase 2, a glycosylasparaginase, a glycosylasparaginase-2, a granzyme, a granzyme A, a granzyme B, a granzyme H, a granzyme K, a granzyme M, a haptoglobin-1, or a combination hereof.


In some embodiments, the protease comprises a histone deacetylase (HDAC), a haptoglobin-related protein, a HAT-like 2, a HAT-like 3, a HAT-like 4, a HAT-like 5, a HAT-related protease, HSP90AA1? (a heat shock 90 kDa protein 1, alpha), HSP90AB1? (a heat shock 90 kDa protein 1, beta), a heat shock protein 75, a heat shock protein 90 kDa beta (Grp94), member 1/tumor rejection antigen (gp96), a hepatocyte growth factor, a hepsin, a HetF-like, a HGF activator, a hGPI8, a Hin-1/OTU domain containing 4, a homologue ICEY, a HP43.8KD, a HTRA1 serine protease, a HTRA2, a HTRA3, a HTRA4, a hyaluronan-binding ser-protease, a implantation serine protease 2, a indian hedgehog protein, a insulysin, a intestinal serine protease 1, a josephin-1, a josephin-2, or a combination hereof.


In some embodiments, the protease comprises a Kallikrein (KLK), a kallikrein hK1, a kallikrein hK2, a kallikrein hK3, a kallikrein hK4, a kallikrein hK5, a kallikrein hK6, a kallikrein hK7, a kallikrein hK8, a kallikrein hK9, a kallikrein hK10, a kallikrein hK11, a kallikrein hK12, a kallikrein hK13, a kallikrein hK14, a kallikrein hK15, a Kell blood-group protein, a KHNYN KH and NYN domain containing, a lactotransferrin, a legumain, a leishmanolysin-2, a leucyl aminopeptidase, a leucyl-cystinyl aminopeptidase, a leukotriene A4 hydrolase, a lysosomal carboxypeptidase A, a lysosomal Pro-X C-peptidase, or a combination hereof.


In some embodiments, the protease comprises a membrane metallo-endopeptidase (MME), a macrophage elastase, a macrophage-stimulating protein, a mammalian tolloid-like 1 protein, a mammalian tolloid-like 2 protein, a MAP1D methione aminopeptidase 1D, a marapsin, a marapsin 2, a MASP1/3 (a MBL associated serine protease 3), a MBL associated serine protease 2 (MASP2), a mastin, a matrilysin, a matrilysin-2, a matriptase, a matriptase-2, a matriptase-3, a membrane dipeptidase, a membrane dipeptidase 2, a membrane dipeptidase 3, a membrane-type mosaic Ser-protein, a meprin alpha subunit, a meprin beta subunit, a mesoderm-specific transcript, a mesotrypsin, a methionyl aminopeptidase I, a methionyl aminopeptidase II, a methionyl aminopeptidase II-like, a mitochondrial inner membrane protease 2, a mitochondrial Intermediate peptidase, a mitochondrial Proc. peptidase b-subunit, a mitochondrial proc. protease, a mitochondrial signal peptidase, a matrix metalloproteinase (MMP), a MMP19, a MMP21, a MMP23A, a MMP23B, a MMP27, a MPND, a MT1-MMP, a MT2-MMP, a MT3-MMP, a MT4-MMP, a MT5-MMP, a MT6-MMP, a MYSM1, or a combination hereof.


In some embodiments, the protease comprises a NAALADASE II, a NAALADASE like 2, a NAALADASE like 1, a napsin A, a napsin B, a nardilysin, a nasal embryonic LHRH factor, a NEDD4 binding protein 1, a neprilysin, a neprilysin-2, a neurolysin, a neurotrypsin, a neutrophil elastase (ELANE, ELA2), a NLRP1 self-cleaving protein, a nuclear recept. interacting protein 2, a nuclear recept. interacting protein 3, a nucleoporin 98, a NYN domain and retroviral integrase containing, a NY-REN-60, an OMA1, an O-sialoglycoprotein endopeptidase, an O-sialoglycoprotein endopeptidase like 1, an osteoblast serine protease, an OTU domain containing 6B, an OTU domain containing-1, an OTU domain containing-3, an OTU domain containing-5, an OTU domain containing-6A, an otubain-1, an otubain-2, an OTUD2/YOD1, an ovastacin, an oviductin-like/ovochymase-2, an ovochymase-like, or a combination hereof.


In some embodiments, the protease comprises a proteinase 3 (PRTN3), a papain, a PACE4 proprotein convertase, a pancreatic elastase, a pancreatic elastase II (IIA), a pancreatic elastase II form B, a pancreatic endopeptidase E (A), a pancreatic endopeptidase E (B), a pappalysin-1, a pappalysin-2, a paracaspase, a paraplegin, a pepsin A, a pepsin C, a PHEX endopeptidase, a PIDD auto-processing protein unit 1, a PIM1 endopeptidase, a PIM2 endopeptidase, a pitrilysin metalloproteinase 1, a plasma Glu-carboxypeptidase, a plasma kallikrein, a plasma-kallikrein-like 2, a plasma-kallikrein-like 3, a plasma-kallikrein-like 4, a plasmin (plasminogen), a PM20D2 peptidase, a POH1/PSMD14, a polyserase-2, a polyserase-3, a polyserase-I, a Ppnx, a presenilin 1, a presenilin 2, a presenilin homolog 1/SPPL3, a presenilin homolog 2, a presenilin homolog 3/SPP, a presenilin homolog 4/SPPL2B, a presenilin homolog 5, a presenilins-assoc. rhomboid like, a procollagen C-proteinase, a proliferation-association protein 1, a prolyl oligopeptidase, a prolyl oligopeptidase-like, a proprotein convertase 1, a proprotein convertase 2, a proprotein convertase 4, a proprotein convertase 5, a proprotein convertase 7, a proprotein convertase 9 (a proprotein convertase subtilisin/kexin type 9, PCSK9), a prostasin, (a protease, serine, 56), a proteasome alpha 1 subunit, a proteasome alpha 2 subunit, a proteasome alpha 3 subunit, a proteasome alpha 3-like subunit, a proteasome alpha 4 subunit, a proteasome alpha 5 subunit, a proteasome alpha 6 subunit, a proteasome alpha 7 subunit, a proteasome alpha 8 subunit, a proteasome b subunit LMP7-like, a proteasome beta 1 subunit, a proteasome beta 2 subunit, a proteasome beta 3 subunit, a proteasome beta 3-like subunit, a proteasome beta 4 subunit, a proteasome catalytic sub. 1-like, a proteasome catalytic subunit 1, a proteasome catalytic subunit 1i, a proteasome catalytic subunit 2, a proteasome catalytic subunit 2i, a proteasome catalytic subunit 3, a proteasome catalytic subunit 3i, a protein C, a protein C-like, a protein Z, a proteinase 3, a PRPF8, a PSMD7, a pyroglutamyl-peptidase I, a pyroglutamyl-peptidase II, or a combination hereof.


In some embodiments, the protease comprises a reelin, a renin, a retinol binding protein 3, a rhomboid 5 homolog 1, a rhomboid 5 homolog 2, a rhomboid domain containing 1, a rhomboid domain containing 2, a rhomboid, veinlet-like 2, a rhomboid, einlet-like 3, a rhomboid-like protein 1, or a combination hereof.


In some embodiments, the protease comprises a serine protease, a serine protease 3 (PRSS3), a S2P protease, a SAD1, a secernin-1, a secernin-2, a secernin-3, a sentrin (SUMO protease 1), a sentrin (SUMO protease 2), a sentrin (SUMO protease 3), a sentrin (SUMO protease 5), a sentrin (SUMO protease 5-like 1), a sentrin (SUMO protease 6), a sentrin (SUMO protease 7), a sentrin (SUMO protease 8), a sentrin (SUMO protease 9), a sentrin (SUMO protease 11), a sentrin (SUMO protease 12), a sentrin (SUMO protease 13), a sentrin (SUMO protease 14), a sentrin (SUMO protease 15), a sentrin (SUMO protease 16), a sentrin (SUMO protease 17), a sentrin (SUMO protease 18), a sentrin (SUMO protease 19), a separase, a seprase, a serine carboxypeptidase 1, a signalase 18 kDa component, a signalase 21 kDa component, a signalase-like 1, a similar to Arabidopsis Ser-prot., a similar to SPUVE, a site-1 protease, a sonic hedgehog protein, a spinesin, a SprT-like N-terminal domain, a stromelysin 1, a stromelysin 2, a stromelysin 3, a suppressor of Ty 16 homolog, or a combination hereof.


In some embodiments, the protease comprises a taspase, a TBP-associated factor 2, a TESP2, a TESP3, a testase 2, a testis serine protease 2, a testis serine protease 3, a testis serine protease 4, a testis serine protease 5, a testis serine protease 6, a testisin, a testis-specific protein tsp50, a thimet oligopeptidase, a thrombin, a thymus-specific serine peptidase, a TINAG related protein, a TMPRSS11A, a t-plasminogen activator, a TRAF-binding protein domain, a transferrin receptor 2 protein, a transferrin receptor protein, a transmembrane Ser-protease 3, a transmembrane Ser-protease 4, a transthyretin, a TRH-degrading ectoenzyme, a tripeptidyl-peptidase I, a tripeptidyl-peptidase II, a trypsin, a trypsin 10, a trypsin 15, a trypsin C, a trypsin X2, a tryptase, a tryptase alpha/beta 1, a tryptase beta 2, a tryptase delta 1, a tryptase gamma 1, a tryptase homolog 2/EOS, a tryptase homolog 3, a tubulointerstitial nephritis antigen, or a combination hereof.


In some embodiments, the protease comprises a ubiquitin C-term. hydrolase BAP1, a ubiquitin C-terminal hydrolase 1, a ubiquitin C-terminal hydrolase 3, a ubiquitin C-terminal hydrolase 4, a ubiquitin C-terminal hydrolase 5, a ubiquitin specific peptidase like 1, a UCR1, a UCR2, a UDP-N-acetylglucosaminyltransferase subunit, a Ufm-1 specific protease 1, a Ufm-1 specific protease 2, a urokinase (PLAU, uPA) a umbilical vein proteinase, a u-plasminogen activator, a USP1, a USP2, a USP3, a USP4, a USP5, a USP6, a USP7, a USP8, a USP9X, a USP9Y, a USP10, a USP11, a USP12, a USP13, a USP14, a USP15, a USP16, a USP17, a USP17-like, a USP18, a USP19, a USP20, a USP21, a USP22, a USP24, a USP25, a USP26, a USP27, a USP28, a USP29, a USP30, a USP31, a USP34, a USP35, a USP36, a USP37, a USP40, a USP41, a USP42, a USP43, a USP44, a USP45, a USP46, a USP47, a USP48, a USP49, a USP50, a USP51, a USP52, a USP53, a USP54, or a combination hereof.


In some embodiments, the protease comprises a VCP (p97)/p47-interacting protein, a VDU1, a vitellogenic carboxypeptidase-L, a X-Pro dipeptidase, a X-prolyl aminopeptidase 2, a YME1-like 1, a zinc finger CCCH-type containing 12A, a zinc finger CCCH-type containing 12B, a zinc finger CCCH-type containing 12C, a zinc finger CCCH-type containing 12D, a Zinc finger containing ubiquitin peptidase 1, or a combination hereof.


In some embodiments, the protease comprises an A20 (Tumor necrosis factor, alpha-induced protein 3, TNF a-induced protein 3). A20 is a zinc finger protein and a deubiquitinating enzyme. A20 has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis, limit inflammation.


In some embodiments, the protease comprises an Angiotensin-converting enzyme 2 (ACE2). ACE2 is an enzyme attached to the membrane cells located to the membrane of cells located in the intestines, kidney, testis, gallbladder, and heart. ACE2 counters the activity of the related angiotensin-converting enzyme, ACE, by reducing the amount of angiostatin II.


In some embodiments, the protease comprises a cathepsin. The cathepsin may be, but is not limited to, a cathepsin A (CTSA), a cathepsin B (CTSB), a cathepsin C (CTSC), a cathepsin D (CTSD), a cathepsin E (CTSE), a cathepsin H (CTSH), a cathepsin K (CTSK), a cathepsin L (CTSL), a cathepsin S (CTSS), a cathepsin V (CTSV), and a cathepsin Z (CTSZ). Cathepsins are a subset of proteases, many of which become activated in low pH. Cathepsisns comprise serine proteases, cysteine proteases, and aspartyl proteases, among others. Cathepsins have been implicated in cancer, Alzheimer's disease, arthritis, Ebola, pancreatitis, glaucoma, COPD, and other diseases.


In some embodiments, the protease comprises a caspase. The caspase may be, but is not limited to, a caspase 1, a caspase 2, a caspase 3, a caspase 4, a caspase 5, a caspase 6, a caspase 7, a caspase 8, a caspase 9, a caspase 10, a caspase 11, a caspase 12, a caspase 13, and a caspase 14.


In some embodiments, the protease comprises a calpain. The calpain may be, but is not limited to a calpain 1, a calpain 2, a calpain 3, a calpain 4, a calpain 5, a calpain 6, a calpain 7, a calpain 8, a calpain 9, a calpain 10, a calpain 11, a calpain 12, a calpain 13, a calpain 14, and a calpain 15. Caspases are a family of protease enzymes that play essential roles in programmed cell death and cell homeostasis.


In some embodiments, the protease comprises a cysteine protease. Cysteine proteases, also known as thiol proteases, are hydrolase enzymes that degrade proteins. These proteases share a common catalytic mechanism that involves a nucleophilic cysteine thiol in a catalytic triad or dyad. The cysteine protease family comprises Papain (Carica papaya), bromelain (Ananas comosus), cathepsin K (liverwort), calpain (Homo sapiens), aspase-1 (Rattus norvegicus), separase (Saccharomyces cerevisiae), Adenain (human adenovirus type 2), Pyroglutamyl-peptidase I (Bacillus amyloliquefaciens), Sortase A (Staphylococcus aureus), Hepatitis C virus peptidase 2 (hepatitis C virus), Sindbis virus-type nsP2 peptidase (sindbis virus), Dipeptidyl-peptidase VI (Lysinibacillus sphaericus), DeSI-1 peptidase (Mus musculus), TEV protease (tobacco etch virus), Amidophosphoribosyltransferase precursor (Homo sapiens), Gamma-glutamyl hydrolase (Rattus norvegicus), Hedgehog protein (Drosophila melanogaster) and DmpA aminopeptidase (Ochrobactrum anthropi), etc.


In some embodiments, the protease comprises a complement C1r serine protease (Complement component 1r). In some embodiments, the protease comprises a complement C1s serine protease (Complement component is). C1r along with C1q and C1s form the C1 complex. C1r has very narrow trypsin-like specificity that is responsible for activation of the C1 complex. C1 activation is a two-step process involving (1) C1r intramolecular autoactivation and (2) C1s cleavage by activated C1r. C1r contains a chymotrypsin-like serine protease domain at its C-terminal, and cleaves a single Arg-Ile bond in C1r and in C1s. Zvi Fishelson, in xPharm: The Comprehensive Pharmacology Reference, 2007.


In some embodiments, the protease comprises a chymotrypsin (chymotrypsins A and B, alpha-chymar ophth, avazyme, chymar, chymotest, enzeon, quimar, quimotrase, alpha-chymar, alpha-chymotrypsin A, alpha-chymotrypsin)). Chymotrypsin is a digestive enzyme component of pancreatic juice acting in the duodenum, where it performs proteolysis, the breakdown of proteins and polypeptides. Chymotrypsin preferentially cleaves peptide amide bonds where the side chain of the amino acid N-terminal to the scissile amide bond is a large hydrophobic amino acid (tyrosine, tryptophan, and phenylalanine).


In some embodiments, the protease comprises a chymase (mast cell protease 1, skeletal muscle protease, skin chymotryptic proteinase, mast cell serine proteinase, skeletal muscle protease). Chymases are a family of serine proteases found in mast cells, basophil granulocytes. Chymases show broad peptidolytic activity and are involved in inflammatory response, hypertension and atherosclerosis.


In some embodiments, the protease comprises a dipeptidyl peptidase (DPP). DPP comprises cathepsin C (DPP1), DPP2, DPP3, DPP4, DPP 6, DPP7, DPP8, DPP9, DPP10.


In some embodiments, the protease comprises a DPP4 (adenosine deaminase complexing protein 2, CD26). DPP4 is expressed on cell surface and is associated with immune regulation, signal transduction, and apoptosis. DPP4 is a serine exopeptidase that cleaves X-proline or X-alanine dipeptides from the N-terminus of polypeptides. DPP-4 is known to cleave a broad range of substrates including growth factors, chemokines, neuropeptides, and vasoactive peptides. DPP4 plays a major role in glucose metabolism, is responsible for the degradation of incretins such as GLP-1, and appears to work as a suppressor in the development of some tumors


In some embodiments, the protease comprises a DPP1 (Cathepsin C, CTSC). DPP1 is a lysosomal exo-cysteine protease belonging to the peptidase C1 family. Cathepsin C appears to be a central coordinator for activation of many serine proteases in immune/inflammatory cells. Cathepsin C catalyzes excision of dipeptides from the N-terminus of protein and peptide substrates,


In some embodiments, the protease comprises a disintegrin and metalloproteinase (ADAM). ADAMs are a family of single-pass transmembrane and secreted metalloendopeptidases. Not all human ADAMs have a functional protease domain. Those ADAMs which are active proteases are classified as sheddases because they cut off or shed extracellular portions of transmembrane proteins.


In some embodiments, the protease comprises an ADAM12 metalloprotease. ADAM12 binds insulin growth factor binding protein-3 (IGFBP-3), appears to be an early Down syndrome marker, and has been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.


In some embodiments, the protease comprises a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS). ADAMTS is a family of multidomain extracellular protease enzymes, comprising ADAMTS1, ADAMTS2, ADAMTS3, ADAMTS4, ADAMTS5 (=ADAMTS11), ADAMTS6, ADAMTS7, ADAMTS8 (or METH-2), ADAMTS9, ADAMTS10, ADAMTS12, ADAMTS13, ADAMTS14, ADAMTS15, ADAMTS16, ADAMTS17, ADAMTS18, ADAMTS19, and ADAMTS20. Known functions of the ADAMTS proteases include processing of procollagens and von Willebrand factor as well as cleavage of aggrecan, versican, brevican and neurocan, making them key remodeling enzymes of the extracellular matrix. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration.


In some embodiments, the protease comprises an ADAMTS1. ADAMTS1 is a member of the ADAMTS protein family. The expression of ADAMTS1 may be associated with various inflammatory processes, development of cancer cachexia, normal growth, fertility, and organ morphology and function.


In some embodiments, the protease comprises a Factor VII activating protease (FSAP). FSAP is a circulating serine protease with high homology to fibrinolytic enzymes, and may be associated with the regulation of coagulation and fibrinolysis.


In some embodiments, the protease comprises a furin. Furin belongs to the subtilisin-like proprotein convertase family, and is a calcium-dependent serine endoprotease. Furin's substrates includes: proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor.


In some embodiments, the protease comprises a histone deacetylase (HDAC). HDACs are a class of enzymes that remove acetyl groups (O═C—CH3) from an ε-N-acetyl lysine amino acid on a histone, allowing the histones to wrap the DNA more tightly.


In some embodiments, the protease comprises a HTRA1 serine protease. HTRA1 is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth.


In some embodiments, the protease comprises a granzyme. Granzymes are serine proteases released by cytoplasmic granules within cytotoxic T cells and natural killer (NK) cells. Granzymes induce programmed cell death in the target cell. Granzymes also kill bacteria and inhibit viral replication.


In some embodiments, the protease comprises, a Kallikrein (KLK). Kallikreins are a subgroup of serine proteases. Kallikreins are responsible for the coordination of various physiological functions including blood pressure, semen liquefaction and skin desquamation.


In some embodiments, the protease comprises a matrix metalloproteinase (MMP, matrix metallopeptidases, matrixins). MPPs are calcium-dependent zinc-containing endopeptidases. MMPs have been implicated in cleavage of cell surface receptors, the release of apoptotic ligands, chemokine/cytokine inactivation, cell proliferation and cell migration.


In some embodiments, the protease comprises a membrane metallo-endopeptidase (MME). MME is a zinc-dependent metalloprotease that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. MME is expressed in a wide variety of tissues and is particularly abundant in kidney. MME is also a common acute lymphocytic leukemia antigen.


In some embodiments, the protease comprises a mannose-binding protein-associated serine protease 2 (MASP2, Mannan-binding lectin serine protease 2, MBL associated serine protease 2). MASP2 is involved in the complement system, cleaves complement components C4 and C2 into C4a, C4b, C2a, and C2b.


In some embodiments, the protease comprises a mannose-binding protein-associated serine protease 3 (MBL associated serine protease 3, MASP3). MASP3 originates from the MASP1 gene through differential splicing, it circulates in high serum concentrations predominantly in complex with Ficolin-3 and regulates Ficolin-3 mediated complement activation.


In some embodiments, the protease comprises a neutrophil elastase (ELANE, ELA2). ELANE is a serine proteinase secreted by neutrophils and microphages during inflammation and destroys bacteria and host tissue.


In some embodiments, the protease comprises a proteinase 3 (PRTN3). PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes and contributes to the proteolytic generation of antimicrobial peptides.


In some embodiments, the protease comprises a plasmin (a.k.a. plasminogen). Plasmin is a proteolytic enzyme derived from an inert plasma precursor known as plasminogen. It is present in blood that degrades many blood plasma proteins, including fibrin clots. In human, plasmin is encoded by PLG gene.


In some embodiments, the protease comprises a pepsin. Pepsin is an endopeptidase that cleaves proteins into smaller peptides. It is an aspartic protease, using a catalytic aspartate in its active site.


In some embodiments, the protease comprises a presenilin-1 (PS-1). PS-1 is a presenilin protein that is one of the four core proteins in the gamma secretase complex, which is considered to play an important role in generation of amyloid beta from amyloid precursor protein.


In some embodiments, the protease comprises a proprotein convertase subtilisin/kexin type 9 (PCSK9). PCSK9 is a member of the peptidase S8 family.


In some embodiments, the protease comprises a serine protease. Serine protease cleaves peptide bonds in proteins, in which serine serves as the nucleophilic amino acid at the enzyme's active site. Serine protease includes many subfamilies.


In some embodiments, the protease comprises a tryptase. Tryptase is a the most abundant secretory granule-derived serine proteinase contained in mast cells and has been used as aa marker for mast cell activation. It is released from mask cells when they are activated as part of a normal immune response as well as in allergic responses.


In some embodiments, the protease comprises, a trypsin. Trypsin is a serine protease from the PA clan superfamily, found in the digestive system. Trypsin cuts peptide chains mainly at the carboxyl side of the amino acids lysine or arginine.


In some embodiments, the protease comprises a urokinase (PLAU, uPA). Urokinase is a serine protease present in humans and other animals. It is present in human urine, blood and in the extracellular matrix of many tissues. It is involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. Urokinase is a 411-residue protein, consisting of three domains: the serine protease domain, the kringle domain, and the EGF-like domain. Urokinase is synthesized as a zymogen form (prourokinase or single-chain urokinase), and is activated by proteolytic cleavage between Lys158 and Ile159. The two resulting chains are kept together by a disulfide bond.


Described herein are agents to be detected including but are not limited to a oxidoreductase, a transferase, a hydrolase, a lyase, a isomerase, a ligase, a protease, a hydrolase, an esterase, a β-glycosidase, a phospholipase and a phosphodiesterase, peroxidase, lipase, amylase a nucleophilic reagent, a reducing reagent, a electrophilic/acidic reagent, an organometallic/metal catalyst, an oxidizing reagent, a hydroxyl ion, a thiols nucleophile, a nitrogen nucleophile, a sodium dithionite and a sodium periodate.


As described herein, the activity detection of some agents does not rely on cleavage. For example, some oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases lead to the substrate linker modification and release or formation of a reporter molecule that can be detected. As a way of illustration, a certain oxidation processes can modify an inactive fluorophore and render it fluorescent/detectable without the need of a substrate linker or binding events (for non-covalent processes) can change magnetic/fluorescent physical-chemical properties of certain reporters and render them detectable.


Disease and Condition


The method described herein comprise determining a disease or condition of the subject. In some aspects, the disease or condition comprises a liver disease, a cancer, a metabolic disease, a fibrotic disease, an organ transplant rejection, an infectious disease, an allergic disease, an autoimmunity, Alzheimer's or a chronic inflammation. In some embodiments, the liver disease may be a non-alcoholic steatohepatitis (NASH), a non-alcoholic fatty liver disease (NAFLD), a toxin mediated liver injury (drug/medication, alcohol, environmental), a viral hepatitis (HAV, HBV, HCV, HDV, HEV, other virus infecting the liver), an autoimmune hepatitis, a primary biliary cholangitis, a primary sclerosing cholangitis, a fulminant hepatitis, a cirrhosis of the liver, a hepatocellular carcinoma (HCC), a cholangiocarcinoma, an acute or chronic rejection of a transplanted liver, an inherited liver disease (e.g. Wilson disease, hemochromatosis, or alpha-1 antitrypsin) or a combination thereof.


In some embodiments, the cancer comprises adenoid cystic carcinoma, adrenal gland tumors, amyloidosis, anal cancer, appendix cancer, astrocytoma, ataxia-telangiectasia, Beckwith-Wiedemann syndrome, bile duct cancer (cholangiocarcinoma), Birt-Hogg-Dube Syndrome, bladder cancer, bone cancer (sarcoma of the bone), brain stem glioma, brain tumors, breast cancer, Carney complex, central nervous system tumors, cervical cancer, colorectal cancer, Cowden Syndrome, craniopharyngioma, Desmoid tumors, desmoplastic infantile ganglioglioma, ependymoma, esophageal cancer, Ewing sarcoma, eye cancer, eyelid cancer, familial adenomatous polyposis, familial GIST, familial malignant melanoma, familial pancreatic cancer, gallbladder cancer, gastrointestinal stromal tumors (GIST), germ cell tumors, gestational trophoblastic disease, head and neck cancer, breast and ovarian cancer, diffuse gastric cancer, leiomyosarcoma and renal cell cancer, mixed polyposis syndrome, papillary renal carcinoma, juvenile polyposis syndrome, kidney cancer, lacrimal gland tumors, laryngeal and hypopharyngeal cancer, leukemia, myeloid leukemia, lymphoblastic leukemia, eosinophilic leukemia, Li-Fraumeni syndrome, liver cancer, lung cancer, Hodgkin lung cancer, non-Hodgkin lung cancer, Lynch syndrome, mastocytosis, medulloblastoma, melanoma, meningioma, mesothelioma, multiple endocrine neoplasia, multiple myeloma, myelodysplastic syndrome, nasal cavity and paranasal sinus cancer, nasopharyngeal cancer, neuroblastoma, neuroendocrine tumors, neurofibromatosis, nevoid basal cell carcinoma syndrome, oral and oropharyngeal cancer, osteosarcoma, ovarian cancer, fallopian tube cancer, peritoneal cancer, pancreatic cancer, parathyroid cancer, penile cancer, Peutz-Jeghers syndrome, phenochromocytoma, paraganglioma, pituitary gland tumors, pleuropulmonary blastoma, prostate cancer, retinoblastoma, rhabdomyosarcoma, salivary gland cancer, Kaposi sarcoma, soft tissue sarcoma, sarcoma, non-melanoma skin cancer, small bowel cancer, stomach cancer, testicular cancer, thymoma and thymic carcinoma, thyroid cancer, tuberous sclerosis complex, uterine cancer, vaginal cancer, von Hippel-Lindau syndrome, vulvar cancer, Waldenstrom macroglobulinemia, Werner syndrome, Wilms tumors, or xeroderma pigmentosum.


In some embodiments, the disease may be NASH. Non-alcoholic steatohepatitis, also called NASH, is a more active inflammatory form of non-alcoholic fatty liver disease (NAFLD). NAFLD is caused by buildup of fat in the liver. When this buildup causes inflammation and damage, it is known as NASH, which can lead to scarring of the liver. There are often no outward signs or symptoms associated with NASH, although the most common symptoms are fatigue or mild pain in the upper right abdomen. NASH may lead to cirrhosis of the liver, causing one or more of the following symptoms as the condition progresses: bleeding easily, bruising easily, itchy skin, jaundice, abdominal fluid accumulation, loss of appetite, nausea, leg swelling, confusion, drowsiness, slurred speech, or spider-like blood vessels.


NASH is most common in patients who are overweight or obese; other risk factors include diabetes, high cholesterol, high triglycerides, poor diet, metabolic syndrome, polycystic ovary syndrome, sleep apnea, and hyperthyroidism.


NAFLD encompasses the entire spectrum of fatty liver disease in individuals without significant alcohol consumption, ranging from fatty liver to steatohepatitis to cirrhosis. Non-alcoholic fatty liver is the presence of >5% hepatic steatosis without evidence of hepatocellular injury in the form of ballooning of the hepatocytes or evidence of fibrosis. The risk of progression to cirrhosis and liver failure is considered minimal. NASH is the presence of >5% hepatic steatosis with inflammation and hepatocyte injury (ballooning) with or without fibrosis. This can progress to cirrhosis, liver failure, and rarely liver cancer. NASH cirrhosis is presence of cirrhosis with current or previous histological evidence of steatosis or steatohepatitis.


NAS is an unweighted composite of steatosis, lobular inflammation, and ballooning scores. NAS is a useful tool to measure changes in liver histology in patients with NAFLD in clinical trials. Fibrosis is scored separately and can be classified as F1 through F4; specifically, stage 1 is zone 3 (perivenular), perisinusoidal, or periportal fibrosis; stage 2 is both zone 3 and periportal fibrosis; stage 3 is bridging fibrosis with nodularity; and stage 4 is cirrhosis.









TABLE 3







The histological scoring system for nonalcoholic fatty liver disease:


components of NAFLD activity score (NAS) and fibrosis staging.










Item
Score
Extent
Definition and Comment










NAS Components (see scoring interpretation)










Steatosis
0
<5%
Refers to amount of surface area involved by steatosis



1
5-33%
as evaluated on low to medium power examination.



2
>33-66%




3
>66%



Lobular
0
No foci
Acidophil bodies are not included in this assessment,


Inflammation
1
<2 foci/200x
nor is portal inflammation



2
2-4 foci/200x




3
>4 foci/200x



Hepatocyte
0
None



Ballooning
1
Few ballooned
“Few” means rare but definite ballooned hepatocytes as




cells
well as cases that are diagnostically borderline



2
Many
Most cases with prominent ballooning also had




cells/prominent
Mallory”s hyalin, but Mallory”s hyaline is not scored




ballooning
separately for the NAS







Fibrosis Stage (Evaluated separately from NAS)










Fibrosis
0
None




1
Perisinusoidal





or periportal




1A
Mild, zone 3,
“delicate” fibrosis




perisinusoidal




1B
Moderate, zone 3,
“dense” fibrosis




perisinusoidal




1C
Portal/periportal
This category is included to accommodate cases with





portal and/or peri portal fibrosis without accompanying





pericellular/perisinusoidal fibrosis



2
Perisinusoidal and





portal/periportal




3
Bridging fibrosis




4
Cirrhosis





Scoring interpretation: Total NAS score represents the sum of scores for steatosis, lobular inflammation, and ballooning, and ranges from 0-8. Diagnosis of NASH (or, alternatively, fatty liver not diagnostic of NASH) should be made first, then NAS is used to grade activity. In the reference study, NAS scores of 0-2 occurred in cases largely considered not diagnostic of NASH, scores of 3-4 were evenly divided among those considered not diagnostic, borderline, or positive for NASH. Scores of 5-8 occurred in cases that were largely considered diagnostic of NASH






In some embodiments, the disease may be NAFLD. Nonalcoholic fatty liver disease (NAFLD) is an umbrella term for a range of liver conditions affecting people who drink little to no alcohol. As the name implies, the main characteristic of NAFLD is too much fat stored in liver cells. There are often no outward signs or symptoms associated with NAFLD, although the most common symptoms are fatigue or mild pain in the upper right abdomen.


In some embodiments, the disease may be fulminant hepatitis. Fulminant hepatitis, or fulminant hepatic failure, is defined as a clinical syndrome of severe liver function impairment, which causes hepatic coma and the decrease in synthesizing capacity of liver. Then they rapidly develop severe, often life-threatening liver failure. This can happen within hours, days, or sometimes weeks. Symptoms of severe liver failure include confusion, extreme irritability, altered consciousness, blood clotting defects, and buildup of fluid in the abdominal cavity and multiorgan system failure.


In some embodiments, the disease may be a hepatocellular carcinoma (HCC). HCC is the most common type of primary liver cancer. HCC occurs most often in people with chronic liver diseases leading to advanced fibrosis or cirrhosis. The most common liver diseases associated with HCC are viral hepatitis B or C, alcohol related liver disease and NASH.


In some embodiments, the disease may be a primary biliary cholangitis (PBC). Primary biliary cholangitis, previously called primary biliary cirrhosis, is a chronic disease in which the bile ducts in the liver are slowly destroyed. Bile is a fluid made in the liver. Chronic inflammation in the liver can lead to bile duct damage, irreversible scarring of liver tissue (cirrhosis) and eventually, liver failure. PBC is considered an autoimmune disease, which means the body's immune system is mistakenly attacking healthy cells and tissue. Researchers think a combination of genetic and environmental factors triggers the disease. It usually develops slowly. At this time, there's no cure for primary biliary cholangitis, but medication can slow liver damage, especially if treatment begins early.


In some embodiments, the liver disease may be a toxin mediated liver injury (e.g., from drug/medication, alcohol, environmental). Toxin mediated liver injury is an inflammation of liver in reaction to certain substances, such as alcohol, chemicals, drugs/medication, environmental factors or nutritional supplements. The liver normally removes and breaks down most drugs and chemicals from the bloodstream, which creates byproducts that can damage the liver. Although the liver has a great capacity for regeneration, constant exposure to toxic substances can cause serious, sometimes irreversible harm.


In some embodiments, the liver disease may be a viral hepatitis (HAV, HBV, HCV, HDV, HEV, other virus infecting the liver). Viral hepatitis is a liver inflammation due to a viral infection. It may present in acute form as a recent infection with relatively rapid onset, or in chronic form. The most common causes of viral hepatitis are the five unrelated hepatotropic viruses hepatitis A, B, C, D, and E. Other viruses can also cause liver inflammation, including cytomegalovirus, Epstein-Barr virus, and yellow fever. There also have been scores of recorded cases of viral hepatitis caused by herpes simplex virus. Viral hepatitis is either transmitted through contaminated food or water (A, E) or via blood and body fluids (B, C). Hepatitis A and hepatitis B can be prevented by vaccination. Effective treatments for hepatitis C are available but costly.


In some embodiments, the liver disease may be an autoimmune hepatitis. Autoimmune hepatitis is liver inflammation that occurs when the immune system attacks liver cells. The exact cause of autoimmune hepatitis is unclear, but genetic and environmental factors appear to interact over time in triggering the disease. Untreated autoimmune hepatitis can lead to scarring of the liver (cirrhosis) and eventually to liver failure. When diagnosed and treated early, autoimmune hepatitis often can be controlled with drugs that suppress the immune system. A liver transplant may be an option when autoimmune hepatitis doesn't respond to drug treatments or in cases of advanced liver disease. There are two main forms of autoimmune hepatitis: (1) Type 1 autoimmune hepatitis. Type I autoimmune hepatitis is the most common type and can occur at any age. About half the people with type 1 autoimmune hepatitis have other autoimmune disorders, such as celiac disease, rheumatoid arthritis or ulcerative colitis; (2) Type 2 autoimmune hepatitis. Although adults can develop type 2 autoimmune hepatitis, it's most common in children and young people. Other autoimmune diseases may accompany type 2 autoimmune hepatitis.


In some embodiments, the liver disease may be a primary sclerosing cholangitis. Primary sclerosing cholangitis is a disease of the bile ducts. In primary sclerosing cholangitis, inflammation causes scars within the bile ducts. These scars make the ducts hard and narrow and gradually cause serious liver damage. A majority of people with primary sclerosing cholangitis also have inflammatory bowel disease, such as ulcerative colitis or Crohn's disease. In most cases of primary sclerosing cholangitis, the disease progresses slowly. It can eventually lead to liver failure, repeated infections, and tumors of the bile duct or liver.


In some embodiments, the liver disease may be a cirrhosis of the liver. Cirrhosis is a late stage of scarring (fibrosis) of the liver caused by many forms of liver diseases and conditions, such as hepatitis and chronic alcoholism. In the process of liver self-repair, scar tissue forms. As cirrhosis progresses, more and more scar tissue forms, making it difficult for the liver to function (decompensated cirrhosis).


In some embodiments, the liver disease may be a cholangiocarcinoma. Cholangiocarcinoma (bile duct cancer) is a type of cancer that forms in the bile ducts. Risk factors for cholangiocarcinoma include primary sclerosing cholangitis (an inflammatory disease of the bile ducts), ulcerative colitis, cirrhosis, hepatitis C, hepatitis B, infection with certain liver flukes, and some congenital liver malformations. Cholangiocarcinoma can be categorized based on the location of the cancer occurs in the bile ducts: intrahepatic cholangiocarcinoma, hilar cholangiocarcinoma, distal cholangiocarcinoma. Cholangiocarcinoma is often diagnosed when it is advanced, making successful treatment difficult to achieve.


In some embodiments, the liver disease may be an inherited liver disease (e.g., Wilson disease, hemochromatosis, or alpha-1 antitrypsin, etc.) Inherited liver diseases are genetic disorders that can cause severe liver disease and other health problems. Wilson's disease is a rare inherited disorder that causes copper to accumulate in your liver, brain and other vital organs. Hemochromatosis is a disease in which deposits of iron collect in the liver and other organs. The primary form of hemochromatosis is one of the most common inherited diseases in the U.S. The alpha-1 antitrypsin protein is synthesized mainly in the liver by hepatocytes, secreted into the blood stream, and acts as an inhibitor of neutrophil elastase released primarily in the lung during inflammation. Alpha-1 antitrypsin deficiency is caused when alpha-1 antitrypsin protein is either lacking or exists in lower than normal levels in the blood.


In some embodiments, the disease may be an organ transplant rejection. Transplant rejection occurs when transplanted tissue is rejected by the recipient's immune system, which destroys the transplanted tissue. Transplant rejection can be lessened by determining the molecular similitude between donor and recipient and by use of immunosuppressant drugs after transplant.


In some embodiments, the disease may be an infectious disease, Infectious diseases are disorders caused by organisms such as bacteria, viruses, fungi or parasites. Bacteria are one-cell organisms responsible for illnesses such as streptococcal upper respiratory infection, urinary tract infections and tuberculosis. Viruses cause a multitude of diseases ranging from the common cold to AIDS. Many skin diseases, such as ringworm and athlete's foot, are caused by fungi. Other types of fungi can infect the lungs or nervous system. Malaria is caused by a tiny parasite that is transmitted by a mosquito bite. Other parasites may be transmitted to humans from animal feces. In some embodiments, the infectious disease is COVID-19.


In some embodiments, the disease may be an allergic disease. Allergic diseases are caused by allergen-induced unfavorable immune responses initiating various symptoms in different organs, which often cannot be completely controlled by modern medicine. The immunologic basis of allergic diseases is observed in two phases: sensitization and development of memory T and B cell responses, and IgE production and effector functions, which are related to eosinophils, innate lymphoid cells, dendritic cell subsets, epithelial cells and tissue inflammation/injury, epithelial barrier, tissue remodeling and chronicity in asthma, atopic dermatitis (AD) and allergic rhinitis (AR). Different disease phenotypes and endotypes may become apparent with different dominant molecular mechanisms, related biomarkers and responses to biologic therapy. Multiple mechanistic factors are complexly involved in the pathogenesis of allergic inflammations


In some embodiments, the disease may be an autoimmune disease/autoimmunity. An autoimmune disease is a condition in which the immune system mistakenly attacks one's own body. Normally, the immune system can tell the difference between foreign cells and one's own cells. In an autoimmune disease, the immune system mistakes part of the body, like the joints or skin, as foreign. It releases proteins called autoantibodies that attack healthy cells. Some autoimmune diseases target only one organ. Type 1 diabetes damages the pancreas. Other diseases, like systemic lupus erythematosus (SLE), affect many different organ systems. In some embodiments, the autoimmune disease may be Rheumatoid arthritis, Crohns disease, Multiple sclerosis (MS) or psoriatic arthritis (PsA).


In some embodiments, the disease may be a chronic inflammation. Chronic inflammation is also referred to as slow, long-term inflammation lasting for prolonged periods of several months to years. Generally, the extent and effects of chronic inflammation vary with the cause of the injury and the ability of the body to repair and overcome the damage. Most of the features of acute inflammation continue as the inflammation becomes chronic, including the expansion of blood vessels (vasodilation), increase in blood flow, capillary permeability and migration of neutrophils into the infected tissue through the capillary wall (diapedesis). However, the composition of the white blood cells changes soon and the macrophages and lymphocytes begin to replace short-lived neutrophils. Thus the hallmarks of chronic inflammation are the infiltration of the primary inflammatory cells such as macrophages, lymphocytes, and plasma cells in the tissue site, producing inflammatory cytokines, growth factors, enzymes and hence contributing to the progression of tissue damage and secondary repair including fibrosis and granuloma formation, etc.


In some embodiments, the disease may be a fibrotic disease. Fibrotic disease is defined by the overgrowth, hardening, and/or scarring of various tissues and is attributed to excess deposition of extracellular matrix components including collagen. Fibrosis is the end result of chronic inflammatory reactions induced by a variety of stimuli including persistent infections, autoimmune reactions, allergic responses, chemical insults, radiation, and tissue injury. The fibrotic disorders include but are not limited to systemic fibrotic diseases such as systemic sclerosis (SSc), sclerodermatous graft vs. host disease, idiopathic pulmonary fibrosis (IPF), nephrogenic systemic fibrosis, and organ-specific disorders including radiation-induced fibrosis and cardiac, pulmonary, liver, and kidney fibrosis.


In some embodiments, the disease may be a metabolic disease. A metabolic disorder/disease occurs when abnormal chemical reactions in the body disrupt metabolism. When this happens, one might have too much of some substances or too little of other ones that an individual needs to stay healthy. There are different groups of disorders. Some affect the breakdown of amino acids, carbohydrates, or lipids. Another group, mitochondrial diseases, affects the parts of the cells that produce the energy. one can develop a metabolic disorder when some organs, such as the liver or pancreas, become diseased or do not function normally. Diabetes is an example.


In some embodiments, the disease may be Alzheimer's. Alzheimer's is a type of dementia that affects memory, thinking and behavior. Symptoms eventually grow severe enough to interfere with daily tasks. Alzheimer's changes typically begin in the part of the brain that affects learning. As Alzheimer's advances through the brain, it leads to increasingly severe symptoms, including disorientation, mood and behavior changes; deepening confusion about events, time and place; unfounded suspicions about family, friends and professional caregivers; more serious memory loss and behavior changes; and difficulty speaking, swallowing and walking.


EXAMPLES

These examples are provided for illustrative purposes only and not to limit the scope of the claims provided herein. It will be appreciated that variations in proportions and alternatives in elements of the components shown will be apparent to those skilled in the art and are within the scope of the embodiments presented herein.


Example 1. Diagnosing NASH Using Probes in Mice

In this experiment, the probes of the present application were shown to accurately detect the activity levels of proteases associated with non-alcoholic steatohepatitis (NASH) in a fluid sample to diagnose NASH in a subject.


Protease activity levels associated with NASH were assessed in vivo in two mice populations, one healthy and one with NASH. The probes used in vivo are shown in FIG. 10.


Mass-barcoded reporters urinary concentration levels obtained from proteolytic cleavage of these probes by proteases in healthy mice, which were fed on a standard Chow Diet (CD), and NASH mice, which were fed a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) are shown in FIG. 11. NASH-related probes, cleaved by increased NASH-related protease activity, associated with higher mass-barcoded reporters accumulation in urine from NASH mice compared to healthy mice.


As shown in FIG. 12, blood samples were collected in K2EDTA tubes from mice that were either healthy (CD) or had NASH (CDAHFD) after 12 weeks on their respective diet. All animals were used in accordance with animal care guidelines. Plasma was obtained from these blood samples by centrifugation at 3,500 RPM for 20 min at 4° C. The plasma was stored at −80° C. until it was needed for experimental purposes.


As shown in FIG. 13, thawed plasma samples were pooled and contacted with probes with fluorescent quenchers and protease-cleavable fluorescent reporters at various peptide and serum concentrations. Samples were mixed with protease substrates and quenchers/reporters in 96-well plates. The 96-well plates were read on a Biotech Synergy H1, using 465,535 excitation/emission settings.


As shown in FIG. 14, the probes of the present application were able to measure the activity of NASH-related proteases as expressed in Relative Fluorescent Unit (RFU) per minute in the two mouse populations. Probes measuring cathepsin activity were 3-fold higher in protease cleavage kinetics in mice with NASH compared to healthy mice. In contrast, probes sensing caspase activity showed no change in detectable activity between healthy and NASH mice. FIG. 15A and FIG. 15B show the subset of results for one probe, Probe #102, in detecting NASH-related protease activity; here, the use of the fluorescent reporter and quencher, like those discussed in FIG. 5, were shown to accurately measure the activity levels of NASH-related proteases in the plasma of healthy mice (FIG. 15A) and NASH mice (FIG. 15B).


Thus, probes of the present application can accurately detect the activity levels of proteases associated with a biological condition or disease-state in a subject, ex vivo, using a body fluid sample.


Example 2: Detection of NASH Protease Activity in Plasma in Mice

As shown in FIG. 14, the probes of the present application are able to accurately detect protease activity of NASH related proteases in the plasma samples taken from two mice populations, as explained in Example 1 and FIG. 13, in a multiplex format. A single plasma sample was contacted with the probes for each predetermined protease to provide a multiplex assessment of protease activity in the sample.


In FIG. 16, for each set of probes, the protease activity in healthy mice is shown on the left, while the protease activity in NASH mice is shown on the right. As shown, the probes of the present application were able to measure increases in NASH-related protease activity.


As shown in FIG. 17 and FIG. 18, protease activity measured as RFU/min was similar in pooled plasma samples within the same group of animals than the average of protease activity from each animal from that group. Furthermore, adding a broad protease inhibitor cocktail (INH) completely abrogated protease activity in both healthy and NASH animal groups, providing evidence that the fluorescent signal measured over time depends on proteolytic activities.



FIG. 19A and FIG. 19B show that, when studying samples of mouse plasma, activity, not abundance, is more important in differentiating between healthy samples and NASH samples. Although abundance of NASH-related proteases (here cathepsin L, or CTSL) may be comparable between healthy CD mice and NASH CDAHFD mice (FIG. 19A), the activity levels of these proteases are not (FIG. 19B). In this experiment, protease abundance was measured using an ELISA kit from LS Bio while activity was measured using the Probe #102 (a CTSL sensing probe) fluorescence assay described in Example 1.


Thus, probes of the present application can accurately detect the activity levels of proteases associated with a biological condition or disease-state in a subject, ex vivo, using a body fluid sample such as plasma in a multiplex format.


Example 3: Liquid Biopsy Determines Progression Versus Regression of NASH

In this experiment, the probes of the present application were able to differentiate among healthy mice, NASH mice, and NASH mice that were undergoing disease regression.



FIG. 20 shows the experimental design including three groups of mice: CDAHFD NASH mice for 20 weeks (NASH progression), healthy CD mice for 20 weeks, and mice fed a CDAHFD for 16 weeks before being switched to a chow diet for 4 weeks (NASH regression). Plasma samples were collected from all animals at 20 weeks.


As seen in FIGS. 21A-F, several probes were used to contact the thawed plasma, as described in Example 1, and this resulted in clear differentiation between the healthy, regression, and NASH samples. The probes showing the most differentiation in NASH were linked to cathepsin and/or MMP protease activities.


This experiment showed that not only can we differentiate between healthy and diseased samples, but it can also differentiate among healthy, disease-progressing, and disease-regressing samples.


Example 4: Liquid Biopsy Applications Towards Fulminant Hepatitis in Mice

In this experiment, another mouse liver-disease model—that for fulminant hepatitis—was studied to determine the wider uses of the present application. This experiment served to develop the ex vivo assay technology for applications in hepatitis models using plasma and existing sensors in the FRET substrate library.


Fulminant hepatitis is induced after injection intraperitoneal of monoclonal antibody anti-CD95 (Jo2, BD biosciences, 4 ug/animal), and mouse plasma samples were collected 3 hours after Jo2 injection. As shown in FIG. 22, when the probes contacted the mouse plasma samples using the method described previously in Example 1, the probes were able to differentiate between healthy and Jo2 samples ex vivo. FIG. 23 shows the same results in vivo, with the same mice receiving the injectable probe formulation for direct comparison with the ex vivo approach.


The Jo2 hepatitis model demonstrates differential probe cleavage compared to NASH liver model data in mice. Predominantly Caspase centric probes (Probe #647, Probe #8, Probe #12) show contrast that is specific and sensitive to the Jo2 model. The comparison with mass spectrometry data also aligns and confirms high concordance with the ex vivo approach, which is reassuring to confirm the existence of a biologically relevant signal.



FIG. 24 demonstrates that for two preclinical models of liver disease, the application can distinctly identify each disease due to the distinct biological mechanisms underlying protease activity of each disease (i.e., cathepsin activity in NASH and caspase activity in hepatitis).


Example 5: Detecting NASH in Human Plasma

This experiment relates to the detection of NASH in humans.


As shown in FIG. 25, blood samples were collected from human subjects that were diagnosed as healthy/lean, healthy/obese, or NASH. Plasma was obtained from these blood samples in the same method as used in Example 1. The plasma was stored at −80 C for no more than 2 years and with a freeze/thaw cycle count of <1 for each sample.


As shown in FIG. 26, when the probes contacted the human plasma samples using the method described in Example 1, increased fluorescence levels over time were observed in NASH samples when compared to healthy, allowing differentiation between the protease activity levels of healthy and NASH samples.



FIG. 27 shows high levels of reproducibility in the application's ability to differentiate between healthy and NASH samples when independent sample cohorts were tested.



FIG. 28 further demonstrates that the application is not only able to differentiate between healthy and NASH human samples, but it is, surprisingly, also able to differentiate between early-stage (F0-F2) and late-stage (F3+) NASH. The entire F0-F4 data set contains 100 NASH samples, and the experiment was conducted using the method from Example 1.


As shown in FIG. 29, multiple probes of the present application are able to differentiate between healthy and NASH samples in humans—this multiplicity furnishes a lower false-positive rate when testing samples


This experiment demonstrates the application is highly adept at differentiating between healthy and NASH (and different fibrosis stages of NASH) in a non-invasive manner in human subjects.


Example 6: Mechanism of Function of Liquid Biopsy

In this experiment, the specific protease cleaved by a specific probe is determined in order to show the specificity of the application regarding the disease differences it detects. This experiment also shows that protease activity, not abundance, is the driving factor in the application's determination of disease-markers in a sample.


For this experiment, all plasma samples were prepared individually and diluted 1/10e in PBS with inhibitor added directly to the samples. Inhibitor was prepared at 15× concentration to final. Substrates were diluted in DI water at 18 uM, such that the final concentration on the plate would be 6 uM. All samples were prepared such that their last dilution on the plate would not affect the desired final concentration. 10 ul of each individual sample was pipetted into their corresponding wells, and the plate was then spun down in the centrifuge at 1500 RPM for 30 seconds to coat the bottom of each well with the sample. 5 ul of the 18 uM substrate solution was pipetted into each well being used on a 384 well plate, and then the plate was spun down in the centrifuge at 1500 rpm for 30 seconds. The plate was placed immediately in the plate reader at 37° C. for a 30-minute-long fluorescence kinetic read at 485/535 extended gain.


To assess the proteolytic cleavage pattern of Probe #102, samples were tested using a pool of broad inhibitors for serine, cysteine, threonine, MMP and aspartic protease family members (broad inhibitor) to assess general protease activity, AEBSF for serine proteases, E64 for cysteine proteases, CTSi for broad cathepsin inhibition of cathepsins L, S, K and B, or specific inhibitors for cathepsin K (L00625), for cathepsin L (SID) or cathepsin B (CA074).


All E64 (broad cysteine), SID (CTSL) and the CTSi (CTSL, S, K, B) inhibitors decreased NASH signal significantly with less decrease in signal for healthy, indicating that the nature of the decrease in signal was disease-specific. When using the broad inhibitor or E64, we observed a greater than 6-fold decrease in the RFU signal contrast between NASH and healthy samples, indicating that a cysteine protease was responsible for the disease contrast. Broad cathepsin inhibitor CTSi decreased NASH by 47% while only decreasing healthy by 18%, demonstrating that a cathepsin was responsible for the disease contrast. A specific cathepsin inhibitor for CTSL (SID) decreased NASH by 60% while only decreasing healthy by 33%. Both NASH and healthy decreased with the addition of the serine inhibitor, AEBSF. NASH was inhibited 65%, while healthy was inhibited at 60%. The similar decrease in RFU for both NASH and healthy indicates that the AEBSF signal being sensed by Probe #102 is not a significant contributor to the disease specific signal and of a background nature.


Specific inhibitors for cathepsin K and B, L006235 and CA074, respectively, did not significantly decrease signal for NASH or healthy samples.



FIG. 30A demonstrates Probe #102 in combination with broad protease inhibitors to show that Probe #102 specifically contacts a protease in order to determine the difference between healthy and NASH samples. FIG. 30B shows that Probe #102 contacts a cysteine protease, and FIG. 30C further limits this to a cathepsin family protease. FIG. 30D-F test individual cathepsins to show that Probe #102 specifically responds to the activity of cathepsin L (CTSL), a NASH-related protease. Thus, cathepsin L activity is responsible for the disease vs. healthy differences in protease activity in samples as recognized by the application.


As shown in FIG. 31A-B, the application's discrimination between healthy and NASH tissue is not caused by either trypsin or thrombin, both promiscuous proteases that are constantly present in blood.


As shown in FIG. 32A-B, protease activity is the true measure of disease, rather than protease quantity. This corroborates the previous determination in mice that activity is more important than abundance as previously seen in Example 2 and as previously shown in FIG. 19.


More specifically, FIG. 33 demonstrates that although CTSL is equally abundant in both healthy and NASH human samples, CTSL activity is different between these two sample populations.


The application is shown to function by measuring the activity levels, rather than the abundance of specific disease-related proteases, to give an accurate determination of a specific disease in a sample.


Example 7: Liquid Biopsy Applications Toward COVID Diagnosis

In this example, the application is directed toward diagnosing COVID.


Initial experiments with COVID used K2EDTA and Lithium Heparin collected plasma. Samples were thawed on ice from storage in −80° C. and were then diluted to 10% in PBS. After the samples were prepared, the volume was split in half and broad protease inhibitors were added to one tube—100× dilution final, 67× in the tube. 10 uL of each sample were placed into a well in a 96-well plate, and the plates were stored on ice. Substrates were prepared at 18 uM in ddH2O using 1 mM stock prepared in DMF. 5 uL of substrate were added to each well. The 96-well plates were spun down at 1000 RPM for <30 seconds. The plates were read on Biotek Synergy H1 plate reader, Ex/Em=485/535 with a cycling time of 4 mins 30 seconds using a kinetic read, extended dynamic range for 1 hour.


As shown in FIG. 34A-B, multiple sensors demonstrated differential cleavage between COVID and healthy samples. Probe #462, Probe #18 and Probe #84 demonstrated contrast in both sets and Probe #409, the SARS CoV2 coronavirus substrate, showed modest contrast in the K2 EDTA samples.


As shown in FIG. 35, COVID positive and COVID negative swabs (as determined by PCR at the clinical site) were combined with LBx sensors to determine if protease activity can be sensed ex vivo using swabs.


Samples were thawed on ice and then diluted to 10% in DPBS (neutral pH 7.4, Gibco). Where required, samples were pooled according to condition with equal volumes of each sample per condition and then subsequently diluted in DPBS. After the samples are prepared, the volume was split in half and broad protease inhibitors were added to 1 tube—100× dilution final, 67× in the tube. 10 uL of each sample was added into the corresponding wells of a 96-well plate, and the plates were stored on ice. Substrates were prepared at 18 uM in ddH2O using 1 mM stock prepared in DMF. 5 uL of substrate was added to each sample in the 96-well plate, and the plates were spun down at 1000× rpm for <30 seconds. Plates were read on a Biotek Synergy H1 plate reader, Ex/Em=485/535 with a cycling time of 4 mins 30 seconds using a kinetic read, extended dynamic range for 2 hours. FIGS. 36A-B shows both swabs and saliva samples treated with viral transport media (VTM), which contains some proteases in the serum after contact with the probes of the application. However, when swabs were tested using the method from experiment 1 using a saline media instead of VTM, as shown in FIG. 37, clear differences could be seen between COVID− and COVID+ samples (as determined by clinical PCR testing). The saline media swabs give superior protease activity signal compared to the VTM swabs as they were collected in saline media with no additives. This shows the application has broad applicability across biofluids.


The specific probe, Probe #647, was shown to be a key differentiator between COVID+ and COVID− samples, as shown in FIG. 38A-C.


As shown in FIGS. 39A-B, Probe #647 signal measures the activity of protease Granzyme B to differentiate between healthy and COVID samples. Granzyme B is a protease that is linked to other autoimmune diseases and viral infections, showing the application can be applied to a wide range of disease biology.


Biotin and Probe #647 were conjugated by dissolving stock Probe #647 powder at 2 mM in 50/50 DMF/PBS. Biotin-Maleimide was reconstituted from powder at 100 mM and diluted to the following concentrations—2 mM, 3 mM and 6 mM in PBS. Three reaction mixtures were created with the following molar equivalents: 1) 1:1-10 uL to 10 uL 2 mM Biotin+2 mM Probe #647, 2) 1:1.5-10 to 10 uL 3 mM Biotin+2 mM Probe #647, and 3) 1:3-10 to 10 uL 6 mM Biotin+2 mM Probe #647. Once mixed, these were inverted on a Hula sample mixer for 2 hours at room temperature. Once the conjugation reactions were completed, recombinant proteases and samples were tested using 100 nM recombinant Granzyme B with 6 uM Probe #647-Biotin conjugate from above 3 reactions. These were then incubated for multiple time points—0 mins, 5 minutes, 30 minutes, 1 hour and optional O/N. They were then diluted up 1:20 and paper strips were dipped into the mixture and the paper strip was read visually. Once the activity was confirmed using recombinant proteases, results were confirmed in strong COVID+ saline swab samples and COVID− saline swab samples (as determined by clinical PCR testing). 10 uL of dilute saline swab sample was combined with 5 uL Probe #647-Biotin conjugate and incubated for multiple time points—0 hours and 2 hours. Post-reaction, the sample was diluted 1:20 and read visually with the paper strip.


The use of a paper strip test to monitor Granzyme B activity using the probes of the application is shown in FIG. 40. This point of care test for the detection of protease cleavage of a biotin-tagged 5FAM sensor has implications for disease monitoring and response in real-time.


Example 8: Liquid Biopsy Applications Towards Pancreatic Ductal Adenocarcinoma

In this example, the application is directed toward diagnosing pancreatic ductal adenocarcinoma (PDAC).


As shown in FIG. 41A-B, when human plasma is contacted with the probes of the application using the method from Experiment 1, one can distinguish between the protease activity of healthy and PDAC human plasma samples.


Furthermore, as shown in FIG. 42, the probes are able to differentiate among healthy, PDAC, and pancreatitis samples.


This experiment continues to show that there is broad applicability for the application regarding different types of diseases that have different protease biology.


Example 9: Probes with a Fluorescent Reporter Will Accurately Measure NASH-Related Protease Activity Levels in Mice

In this prophetic experiment, probes of the present disclosure that include a precipitating fluorescent reporter and a protease substrate cleavable by an endoprotease, like the probes discussed in FIG. 8, will be able to accurately measure the activity levels of NASH-related proteases in healthy mice and NASH mice.


The probes will be engineered such that the protease substrate could be cleaved by a protease such as endoprotease caspase 8, thereby resulting in a second protease substrate linked to a precipitating fluorescent reporter by an auto-immolative spacer. Alternatively, the second protease substrate could be cleaved by the endoprotease CTSD.


Spiking the plasma samples with an excess of CTSD would not result in a measured increase in caspase 8 activity. Thus, in the absence of caspase 8 to cleave the protease substrate, the second substrate will be unavailable for cleavage by CTSD, which will ultimately prevent precipitation of the fluorescent reporter.


However, upon addition of small concentrations of caspase 8 to the fluid sample, a strong signal will be detected by the precipitating fluorophores. Thus, caspase 8 will be able to cleave the protease substrate, thereby resulting in the second protease substrate, which will be cleaved by CTSD. This ultimately will lead to dissociation of the spacer from the precipitating fluorescent reporter, thereby resulting in a fluorescent signal.


Plasma samples with probes having distinguishable precipitating fluorescent reporters will be pooled after incubation with caspase 8 and CTSD. Individually, the plasma samples will be taken from either healthy mice or those with NASH to determine the differences between healthy and NASH samples through detection of caspase 8.


Example 10: Detecting Alternative Enzymes

In this experiment, measurement of alternative enzymes” activities for disease detection is explored. Different enzyme classes include peroxidases, lipases, esterases, phospholipases, amylase etc.



FIG. 43 shows a schematic diagram for detection of Chlorination and peroxidation activity of MPO using the EnzChek® Myeloperoxidase Activity Assay Kit. AH represents the nonfluorescent Amplex® UltraRed substrate, and A represents its fluorescent oxidation product. Hydrogen peroxide converts MPO to MPO-I and MPO is inactive without the presence of hydrogen peroxide. Amplex® UltraRed is then oxidized by MPO-I and creates the fluorescent oxidation product A which can be read at Ex/Em=530/590.



FIG. 44A-C shows the results for detecting peroxidases. FIG. 44A shows that MPO activities are different between healthy mice and mice with NASH. FIG. 44B shows that MPO activities are different between mice fed on a standard ChowDiet (CD), and mice fed on a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD). FIG. 44C shows that MPO activities are different between healthy subjects and subjects with rheumatoid arthritis. This result shows that we are capable of detecting differential activity in NASH in plasma and rheumatoid arthritis in human pools in synovial fluid.



FIG. 45A-B shows the pooled results of spiked recombinant protease in human plasma using resorufin oleate as substrate. FIG. 46A shows result of 3 recombinant enzymes—carboxylesterase 1, phospholipase A2 and lipoprotein lipase. FIG. 46B shows the result of various concentrations of lipoprotein lipase. This result demonstrates that Resorufin oleate and butyrate were promising for detection of broad range of enzymes.


While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. It is not intended that the invention be limited by the specific examples provided within the specification. While the invention has been described with reference to the aforementioned specification, the descriptions and illustrations of the embodiments herein are not meant to be construed in a limiting sense. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. Furthermore, it shall be understood that all aspects of the invention are not limited to the specific depictions, configurations or relative proportions set forth herein which depend upon a variety of conditions and variables. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. It is therefore contemplated that the invention shall also cover any such alternatives, modifications, variations or equivalents. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.

Claims
  • 1. A method comprising: contacting a plasma sample from a subject with a synthetic molecule ex vivo, wherein said synthetic molecule comprises a reporter and a cleavable linker, andwherein said synthetic molecule reacts with an agent from said plasma sample,wherein said agent cleaves said cleavable linker causing said reporter to form a detectable signal,introducing an anticoagulant to said plasma sample,detecting a rate of formation or an amount of said detectable signal,determining a disease condition based on said rate of formation or amount of said released reporter;wherein said disease condition comprises a liver disease.
  • 2. The method of claim 1, wherein said cleavable linker is a peptide.
  • 3. The method of claim 2, wherein said peptide comprises an amino acid sequence selected from the group consisting of SEQ ID Nos: 1-677.
  • 4. The method of claim 1, wherein said cleavable linker is directly connected to said reporter through a covalent bond.
  • 5. The method of claim 1, wherein said anticoagulant is an EDTA, a citrate, a heparin, an oxalate, any salt, solvate, enantiomer, tautomer and geometric isomer thereof, or any mixtures thereof.
  • 6. The method of claim 1, wherein said agent is a protease.
  • 7. The method of claim 6, wherein said protease is an endopeptidase or an exopeptidase.
  • 8. The method of claim 6, wherein said protease is selected from the group consisting of an A20 (TNFa-induced protein 3), an abhydrolase domain containing 4, an abhydrolase domain containing 12, an abhydrolase domain containing 12B, an abhydrolase domain containing 13, an acrosin, an acylaminoacyl-peptidase, a disintegrin and metalloproteinase (ADAM), an ADAM1a, an ADAM2 (Fertilin-b), an ADAM3B, an ADAM4, an ADAM4B, an ADAMS, an ADAM6, an ADAM7, an ADAMS, an ADAMS, an ADAM10, an ADAM 11, an ADAM12 metalloprotease, an ADAM15, an ADAM17, an ADAM18, an ADAM19, an ADAM20, an ADAM21, an ADAM22, an ADAM23, an ADAM28, an ADAM29, an ADAM30, an ADAM32, an ADAM33, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), an ADAMTS1, an ADAMTS2, an ADAMTS3, an ADAMTS4, an ADAMTS5/11, an ADAMTS6, an ADAMTS7, an ADAMTS8, an ADAMTS9, an ADAMTS10, an ADAMTS12, an ADAMTS13, an ADAMTS14, an ADAMTS15, an ADAMTS16, an ADAMTS17, an ADAMTS18, an ADAMTS19, an ADAMTS20, an adipocyte-enh. binding protein 1, an Afg3-like protein 1, an Afg3-like protein 2, an airway-trypsin-like protease, an aminoacylase, an aminopeptidase A, an aminopeptidase B, an aminopeptidase B-like 1, an aminopeptidase MAMS/L-RAP, an aminopeptidase N, an aminopeptidase O, an aminopeptidase P homologue, an aminopeptidase P1, an aminopeptidase PILS, an aminopeptidase Q, an aminopeptidase-like 1, an AMSH/STAMBP, an AMSH- LP/STAMBPL1, an angiotensin-converting enzyme 1 (ACE1), an angiotensin-converting enzyme 2 (ACE2), an angiotensin-converting enzyme 3 (ACE3), an anionic trypsin (II), an apolipoprotein (a), an archaemetzincin-1, an archaemetzincin-2, an aspartoacylase, an aspartoacylase-3, an aspartyl aminopeptidase, an ataxin-3, an ataxin-3 like, an ATP/GTP binding protein 1, an ATP/GTP binding protein-like 2, an ATP/GTP binding protein-like 3, an ATP/GTP binding protein-like 4, an ATP/GTP binding protein-like 5, an ATP23 peptidase, an autophagin-1, an autophagin-2, an autophagin-3, an autophagin-4, an azurocidin, a beta lactamase, a beta-secretase 1, a beta-secretase 2, a bleomycin hydrolase, a brain serine proteinase 2, a BRCC36 (BRCA2-containing complex, sub 3), a calpain, a calpain 1, a calpain 2, a calpain 3, a calpain 4, a calpain 5, a calpain 6, a calpain 7, a calpain 7-like, a calpain 8, a calpain 9, a calpain 10, a calpain 11, a calpain 12, a calpain 13, a calpain 14, a calpain 15 (Solh protein), a cysteine protease, a carboxypeptidase A1, a carboxypeptidase A2, a carboxypeptidase A3, a carboxypeptidase A4, a carboxypeptidase A5, a carboxypeptidase A6, a carboxypeptidase B, a carboxypeptidase D, a carboxypeptidase E, a carboxypeptidase M, a carboxypeptidase N, a carboxypeptidase O, a carboxypeptidase U, a carboxypeptidase X1, a carboxypeptidase X2, a carboxypeptidase Z, a carnosine dipeptidase 1, a carnosine dipeptidase 2, a caspase recruitment domain family, member 8, a caspase, a caspase-1, a caspase-2, a caspase-3, a caspase-4/11, a caspase-5, a caspase-6, a caspase-7, a caspase-8, a caspase-9, a caspase-10, a caspase-12, a caspase-14, a caspase-14-like, a casper/FLIP, a cathepsin, a cathepsin A (CTSA), a cathepsin B (CTSB), a cathepsin C (CTSC), a cathepsin D (CTSD), a cathepsin E (CTSE), a cathepsin F, a cathepsin G, a cathepsin H (CTSH), a cathepsin K (CTSK), a cathepsin 1 (CTSL), a cathepsin L2, a cathepsin 0, a cathepsin S (CTSS), a cathepsin V (CTSV), a cathepsin W, a cathepsin Z (CTSZ), a cationic trypsin, a cezanne/OTU domain containing 7B, a cezanne-2, a CGI-58, a chymase, a chymopasin, a chymosin, a chymotrypsin B, a chymotrypsin C, a coagulation factor IXa, a coagulation factor VIIa, a coagulation factor Xa, a coagulation factor XIa, a coagulation factor XIIa, a collagenase 1, a collagenase 2, a collagenase 3, a complement protease C1r serine protease, a complement protease C1s serine protease, a complement C1r-homolog, a complement component 2, a complement component Cira, a complement component C1sa, a complement factor B, a complement factor D, a complement factor D-like, a complement factor I, a COPSE, a corin, a CSN5 (JAB1), a cylindromatosis protein, a cytosol alanyl aminopep.-like 1, a cytosol alanyl aminopeptidase, a DDI-related protease, a DECYSIN, a Derl-like domain family, member 1, a Derl-like domain family, member 2, a Derl-like domain family, member 3, a DESC1 protease, a desert hedgehog protein, a desumoylating isopeptidase 1, a desumoylating isopeptidase 2, a dihydroorotase, a dihydropyrimidinase, a dihydropyrimidinase-related protein 1, a dihydropyrimidinase-related protein 2, a dihydropyrimidinase-related protein 3, a dihydropyrimidinase-related protein 4, a dihydropyrimidinase-related protein 5, a DINE peptidase, a dipeptidyl peptidase (DPP), a dipeptidyl peptidase (DPP1), a dipeptidyl-peptidase 4 (DPP4), a dipeptidyl-peptidase 6 (DPP6), a dipeptidyl-peptidase 8 (DPP8), a dipeptidyl-peptidase 9 (DPP9), a dipeptidyl-peptidase II, a dipeptidyl-peptidase III, a dipeptidyl-peptidase 10 (DPP10), a DJ-1, a DNA-damage inducible protein, a DNA- damage inducible protein 2, a DUB-1, a DUB-2, a DUB2a, a DUB2a-like, a DUB2a-1ike2, a DUB6, or a combination thereof.
  • 9. The method of claim 6, wherein said protease is selected from the group consisting of a T cell protease, a complement protease, a fibrosis protease, and an inflammation-related protease.
  • 10. The method of claim 1, wherein said reporter comprises a fluorescent label.
  • 11. The method of claim 10, wherein said fluorescent label is selected from a group consisting a 5-carboxyfluorescein (5-FAM), a 7-amino-4-carbamoylmethylcoumarin (ACC), a 7-Amino-4-methylcoumarin (AMC), a 2-Aminobenzoyl (Abz), a Cy7, a Cy5, a Cy3 and a (5-((2-Aminoethyl)amino)naphthalene-l-sulfonic acid) (EDANS).
  • 12. The method of claim 10, wherein said synthetic molecule further comprises a fluorescent quencher.
  • 13. The method of claim 12, wherein said fluorescent quencher is selected from the group consisting of BHQO, BHQ1, BHQ2, BHQ3, BBQ650, ATTO 540Q, ATTO 580Q, ATTO 612Q, CPQ2, QSY-21, QSY-35, QSY-7, QSY-9, DABCYL (4-([4′-dimethylamino)phenyl]azo)benzoyl), Dnp (2,4-dinitrophenyl) and Eclipse.
  • 14. The method of claim 1, wherein said synthetic molecule further comprises a carrier.
  • 15. The method of claim 14, wherein said carrier comprises a native, labeled or synthetic protein, a synthetic chemical polymer of precisely known chemical composition or with a distribution around a mean molecular weight, an oligonucleotide, a phosphorodiamidate morpholino oligomer (PMO), a foldamer, a lipid, a lipid micelle, a nanoparticle, a solid support made of polystyrene, polypropylene or any other type of plastic, or any combination thereof.
  • 16. The method of claim 1, wherein said subject is a human subject.
  • 17. The method of claim 1, wherein said detection comprises a fluorescent detection.
  • 18. The method of claim 17, wherein said fluorescent detection is a fluorescence resonance energy transfer (FRET).
CROSS REFERENCE

This application is a continuation-in-part of International Application No. PCT/US2021/049948, filed on Sep. 10, 2021 which claims the benefit of U.S. Provisional Application No. 63/077,525, filed on Sep. 11, 2020, each of which is entirely incorporated herein by reference for all purposes.

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Related Publications (1)
Number Date Country
20220128546 A1 Apr 2022 US
Provisional Applications (1)
Number Date Country
63077525 Sep 2020 US
Continuation in Parts (1)
Number Date Country
Parent PCT/US2021/049948 Sep 2021 US
Child 17573110 US